{"id":450,"date":"2016-07-13T00:00:00","date_gmt":"2016-07-13T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/fast-cloning\/"},"modified":"2024-08-27T11:06:22","modified_gmt":"2024-08-27T02:06:22","slug":"fast-cloning","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/fast-cloning\/","title":{"rendered":"\u305f\u3063\u305f1.5\u6642\u9593\u3067\u5b8c\u4e86\uff01\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u5b9f\u9a13\u306e\u6642\u9593\u3092\u77ed\u7e2e\u3059\u308b Fast Cloning\u3068\u306f\uff1f"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u305f\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u89e3\u6790\u3059\u308b\u305f\u3081\u306b\u3001\u30b5\u30d6\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\uff08\u3042\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u65ad\u7247\u3092\u3001\u5225\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u7d44\u307f\u8fbc\u3080\u3053\u3068\uff09\u306f\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u3067\u5fc5\u9808\u306a\u6280\u8853\u3067\u3059\u3002\u4e00\u822c\u7684\u306a\u5b9f\u9a13\u306e\u6d41\u308c\u306f\u3001\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u306e\u6e96\u5099\u3001\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u5148\u306e\u30d9\u30af\u30bf\u30fc\u306e\u5236\u9650\u9175\u7d20\u51e6\u7406\u3001\u30d9\u30af\u30bf\u30fc\u306e\u8131\u30ea\u30f3\u9178\u5316\u3068alkaline phosphatase (AP)\u306e\u4e0d\u6d3b\u6027\u5316\u3001\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u3068\u306a\u308a\u307e\u3059\uff08\u56f31\uff09\u3002\u5236\u9650\u9175\u7d20\u306e\u7a2e\u985e\u3084\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u306b\u7528\u3044\u308b\u8a66\u85ac\u306a\u3069\u306b\u3088\u3063\u3066\u306f\u3001\u3053\u308c\u3089\u306e\u5de5\u7a0b\u306f1\u65e5\u4ee5\u4e0a\u8981\u3059\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u3053\u306e\u30d7\u30ed\u30bb\u30b9\u306f\u3001\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u3092\u884c\u3046\u969b\u306b\u306f\u307b\u307c\u5fc5\u9808\u3067\u306f\u3042\u308a\u307e\u3059\u304c\u3001\u4ed6\u306e\u91cd\u8981\u306a\u5b9f\u9a13\u306e\u6642\u9593\u3092\u596a\u3063\u3066\u3057\u307e\u3063\u3066\u3044\u308b\u306e\u3082\u4e8b\u5b9f\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n<p>\u305d\u3053\u3067\u4eca\u56de\u306f\u3001<strong>\u3053\u306e\u5de5\u7a0b\u3092\u6700\u77ed\u30671.5\u6642\u9593\u306b\u77ed\u7e2e\u3067\u304d\u308b\u5f0a\u793e\u306e\u88fd\u54c1\u3092\u7528\u3044\u305fFast Cloning\u306e\u65b9\u6cd5\u3092\u7d39\u4ecb\u3057\u307e\u3059<\/strong>\u3002<\/p>\n<p>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<br \/>\n\u30fb\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u5b9f\u9a13\u306b\u591a\u304f\u306e\u6642\u9593\u3092\u8981\u3057\u3066\u3044\u308b\u65b9<br \/>\n\u30fb\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u5b9f\u9a13\u3092\u3088\u308a\u3059\u3070\u3084\u304f\u884c\u3044\u305f\u3044\u65b9<\/p>\n<div id=\"attachment_1294\" style=\"width: 363px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig1.jpg\" rel=\"attachment wp-att-1294\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1294\" class=\"size-full wp-image-1294\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig1.jpg\" alt=\"fast-cloning-fig1\" width=\"353\" height=\"452\" \/><\/a><p id=\"caption-attachment-1294\" class=\"wp-caption-text\">\u56f31 \u30b5\u30d6\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u4e00\u822c\u7684\u306a\u6d41\u308c<\/p><\/div>\n<h2>\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u306e\u6e96\u5099<\/h2>\n<p>\u30b5\u30d6\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u307e\u305a\u5236\u9650\u9175\u7d20\u3084PCR\u3092\u7528\u3044\u3066\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u306e\u6e96\u5099\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n<h4>\u5236\u9650\u9175\u7d20\u51e6\u7406\u3067\u5143\u306e\u30d9\u30af\u30bf\u30fc\u304b\u3089\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u3092\u6e96\u5099\u3059\u308b\u5834\u5408<\/h4>\n<p>\u4e00\u822c\u7684\u306b\u306f2\u7a2e\u985e\u306e\u5236\u9650\u9175\u7d20\u3067\u5207\u65ad\u3057\u307e\u3059\u3002\u5f93\u6765\u306e\u5236\u9650\u9175\u7d20\u3092\u7528\u3044\u305f\u4e8c\u91cd\u5207\u65ad\u306e\u5834\u5408\u3001\u5236\u9650\u9175\u7d20\u306e\u7d44\u307f\u5408\u308f\u305b\u3067\u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u66ff\u3048\u308b\u5fc5\u8981\u304c\u3042\u308a\u3001\u5b8c\u5168\u306a\u5207\u65ad\u306b\u306f\u6570\u6642\u9593\u304b\u3089\u4e00\u663c\u591c\u304b\u304b\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u9577\u6642\u9593\u306e\u53cd\u5fdc\u3067\u306f\u30b9\u30bf\u30fc\u6d3b\u6027*\u3092\u6c17\u306b\u3057\u306a\u304f\u3066\u306f\u3044\u3051\u307e\u305b\u3093\u3002<\/p>\n<p><span style=\"font-size: 8pt\">(\u30b9\u30bf\u30fc\u6d3b\u6027*\uff1a\u5236\u9650\u9175\u7d20\u306e\u7279\u7570\u6027\u304c\u4f4e\u4e0b\u3057\u3066\u3001\u672c\u6765\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u306f\u7570\u306a\u308b\u5869\u57fa\u914d\u5217\u3092\u5207\u65ad\u3057\u3066\u3057\u307e\u3046\u73fe\u8c61\u306e\u3053\u3068\u3002)<\/span><\/p>\n<p>\u4e00\u65b9\u3067\u3001<strong><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/thermo-scientific-restriction-modifying-enzymes\/restriction-enzymes-thermo-scientific\/fastdigest-thermo-scientific.html?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">FastDigest Enzymes<\/a>\u306f\u3001176\u7a2e\u985e\u306e\u3059\u3079\u3066\u306e\u9175\u7d20\u304c1\u3064\u306eFastDigest reaction buffer\uff08\u307e\u305f\u306fFastDigest Green reaction buffer\uff09\u3067100%\u306e\u6d3b\u6027\u3092\u6301\u3064\u305f\u3081\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u66ff\u3048\u308b\u3053\u3068\u306a\u304f\u4e8c\u91cd\u5207\u65ad\u3067\u304d\u307e\u3059<\/strong>\u3002\u307e\u305f\u3001<strong>\u5207\u65ad\u901f\u5ea6\u304c\u901f\u304f5-15\u5206\u3067DNA\u3092\u5207\u65ad\u3067\u304d\u308b<\/strong>\u305f\u3081\u3001\u30b9\u30bf\u30fc\u6d3b\u6027\u3092\u6c17\u306b\u3057\u306a\u304f\u3066\u6e08\u307f\u307e\u3059\uff08\u56f32\uff09\u3002\u3055\u3089\u306bFastDigest Green Buffer\u3092\u7528\u3044\u305f\u53cd\u5fdc\u6db2\u3092\u4f7f\u3048\u3070\u3001\u96fb\u6c17\u6cf3\u52d5\u306e<strong>\u30b2\u30eb\u306b\u76f4\u63a5\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd<\/strong>\u3067\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<div id=\"attachment_1295\" style=\"width: 199px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig2.jpg\" rel=\"attachment wp-att-1295\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1295\" class=\"wp-image-1295 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig2.jpg\" alt=\"fast-cloning-fig2\" width=\"189\" height=\"187\" \/><\/a><p id=\"caption-attachment-1295\" class=\"wp-caption-text\">\u56f32 FastDigest enzymes\u30675\u5206\u9593\u51e6\u7406\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9DNA\u306e\u5358\u304a\u3088\u3073\u4e8c\u91cd\u5207\u65ad\u3002M:\u00a0GeneRuler 1 kb Plus DNA Ladder\u3001C: \u672a\u5207\u65ad\u30d7\u30e9\u30b9\u30df\u30c9DNA\u30011:\u00a0FastDigest\u00a0SmaI\u30675\u5206\u9593\u5207\u65ad\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9DNA\u30012:\u00a0FastDigest\u00a0EcoRI\u30675\u5206\u9593\u5207\u65ad\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9DNA\u30013:\u00a0FastDigest\u00a0SmaI\u3068FastDigest\u00a0EcoRI\u30675\u5206\u9593\u4e8c\u91cd\u5207\u65ad\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9DNA\u3002<\/p><\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h4>\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u3092PCR\u3067\u6e96\u5099\u3059\u308b\u5834\u5408<\/h4>\n<p>\u6b63\u78ba\u6027\u306e\u9ad8\u3044PCR\u9175\u7d20\u3092\u7528\u3044\u307e\u3059\u3002<br \/>\n\u4f8b\u3048\u3070\u3001<i>Pfu<\/i> polymerase\u3092\u7528\u3044\u305f\u5834\u5408\u3001\u6b63\u78ba\u6027\u306f<i>Taq<\/i> polymerase\u3068\u6bd4\u3079\u30668\u500d\u3067\u3059\u3002\u4e00\u65b9\u3001\u3053\u306e\u9175\u7d20\u3067\u306f1.5 kb\u306e\u5897\u5e45\u65ad\u7247\u3092\u4f38\u9577\u3059\u308b\u306e\u306b1\u6642\u9593\u8981\u3057\u307e\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/F530S?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Phusion High-Fidelity DNA Polymerase<\/a>\u306f<i>Pyrococcus<\/i>\u7531\u6765\u306e\u65b0\u898f\u30d7\u30eb\u30fc\u30d5\u30ea\u30fc\u30c7\u30a3\u30f3\u30b0\u9175\u7d20\u306b2\u672c\u9396DNA\u7d50\u5408\u30c9\u30e1\u30a4\u30f3\u3092\u878d\u5408\u3055\u305b\u305fPCR\u9175\u7d20\u3067\u3059\u30022\u672c\u9396DNA\u7d50\u5408\u30c9\u30e1\u30a4\u30f3\u306f\u3001DNA polymerase\u3068DNA\u306e\u9593\u306e\u7d50\u5408\u3092\u7269\u7406\u7684\u306b\u5b89\u5b9a\u5316\u3055\u305b\u30011\u56de\u306e\u7d50\u5408\u3042\u305f\u308a\u306b\u53d6\u308a\u8fbc\u3081\u308b\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u91cf\u3092\u5897\u3084\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u4f38\u9577\u53cd\u5fdc\u306b\u5fc5\u8981\u306a\u7d50\u5408\u30a4\u30d9\u30f3\u30c8\u306e\u56de\u6570\u3092\u6e1b\u3089\u3057\u307e\u3059\u3002\u3053\u306e\u6280\u8853\u306b\u3088\u3063\u3066\u3001<strong><i>Taq<\/i>\u3068\u6bd4\u3079\u306652\u500d\u306e\u6b63\u78ba\u6027\uff08Phusion HF Buffer\u306b\u304a\u3051\u308b\u30a8\u30e9\u30fc\u7387\u306f4.4 x 10-7)\u3067\u30011.5 kb\u306e\u5897\u5e45\u65ad\u7247\u306e\u5897\u5e45\u304c\u6700\u77ed20\u5206\u3067\u5b8c\u4e86\u3057\u307e\u3059<\/strong>\uff08\u56f33\uff09\u3002<\/p>\n<div id=\"attachment_1296\" style=\"width: 423px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig3.jpg\" rel=\"attachment wp-att-1296\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1296\" class=\"size-full wp-image-1296\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig3.jpg\" alt=\"fast-cloning-fig3\" width=\"413\" height=\"294\" \/><\/a><p id=\"caption-attachment-1296\" class=\"wp-caption-text\">\u56f33 \u7570\u306a\u308bPCR\u9175\u7d20\u306e\u6b63\u78ba\u6027\u306e\u6bd4\u8f03\u3002Phusion DNA Polymerase\u306e\u30a8\u30e9\u30fc\u7387\u306fTaq DNA polymerase\u3088\u308a52\u500d\u3001Pfu DNA polymerase\u3088\u308a6.5\u500d\u4f4e\u304f\u306a\u308a\u307e\u3057\u305f\u3002<\/p><\/div>\n<h2>\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u5148\u306e\u30d9\u30af\u30bf\u30fc\u306e\u5236\u9650\u9175\u7d20\u51e6\u7406<\/h2>\n<p>\u6e96\u5099\u3057\u305f\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u3092\u76ee\u7684\u306e\u30d9\u30af\u30bf\u30fc\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u9069\u5207\u306a\u5236\u9650\u9175\u7d20\u306b\u3088\u3063\u3066\u30d9\u30af\u30bf\u30fc\u3092\u5207\u65ad\u3057\u307e\u3059\u3002\u5148\u306b\u8a18\u3057\u305f\u901a\u308a\u3001<strong><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/thermo-scientific-restriction-modifying-enzymes\/restriction-enzymes-thermo-scientific\/fastdigest-thermo-scientific.html?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">FastDigest enzymes<\/a>\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067DNA\u306e\u5207\u65ad\u304c5-15\u5206\u3067\u53ef\u80fd<\/strong>\u3067\u3059\u3002<\/p>\n<h2>\u30d9\u30af\u30bf\u30fc\u306e\u8131\u30ea\u30f3\u9178\u5316\u3068AP\u306e\u4e0d\u6d3b\u6027\u5316<\/h2>\n<p>\u5236\u9650\u9175\u7d20\u51e6\u7406\u5f8c\u306f\u30bb\u30eb\u30d5\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3*\u3092\u9632\u3050\u305f\u3081\u306b\u8131\u30ea\u30f3\u9178\u5316\u51e6\u7406\u3092\u3057\u307e\u3059\u304c\u3001Calf Intestinal Alkaline Phosphatase (CIAP)\u3092\u7528\u3044\u305f\u5834\u5408\u3001\u8131\u30ea\u30f3\u9178\u5316\u3068\u30a2\u30eb\u30ab\u30ea\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u306e\u4e0d\u6d3b\u5316\u53cd\u5fdc\u306e\u6642\u9593\u3092\u5165\u308c\u308b\u3068\u7d041.5\u6642\u9593\u8981\u3057\u307e\u3059\u3002\u4e00\u65b9\u3001<strong><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/EF0654?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">FastAP Thermosensitive Alkaline Phosphatase<\/a>\u3092\u4f7f\u3046\u3068\u300137\u2103\u30fb10\u5206\u3067\u8fc5\u901f\u306b\u8131\u30ea\u30f3\u9178\u5316\u3057\u300175\u2103\u30fb5\u5206\u306e\u9ad8\u6e29\u51e6\u7406\u306b\u3088\u308a\u4e0d\u6d3b\u5316\u3057\u307e\u3059<\/strong>\uff08\u56f34\uff09\u3002\u307e\u305f\u3001FastDigest reaction buffer\u304a\u3088\u3073FastDigest Green reaction buffer\u4e2d\u3067\u6d3b\u6027\u3092\u6301\u3064\u305f\u3081\u3001<strong>\u5236\u9650\u9175\u7d20\u51e6\u7406\u3057\u305fDNA\u306e\u53cd\u5fdc\u6db2\u306b\u76f4\u63a5\u52a0\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059<\/strong>\u3002\u3053\u306e\u6642\u3001\u5236\u9650\u9175\u7d20\u3092\u4e0d\u6d3b\u5316\u3059\u308b\u305f\u3081\u306e\u9ad8\u6e29\u51e6\u7406\u306f\u5fc5\u8981\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<p><span style=\"font-size: 8pt\">\uff08*\u30bb\u30eb\u30d5\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\uff1a\u5207\u65ad\u3057\u305f\u30d9\u30af\u30bf\u30fcDNA\u81ea\u8eab\u304c\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u3059\u308b\u3053\u3068\u3002\uff09<\/span><\/p>\n<div id=\"attachment_1297\" style=\"width: 230px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig4.jpg\" rel=\"attachment wp-att-1297\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1297\" class=\"size-full wp-image-1297\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig4.jpg\" alt=\"fast-cloning-fig4\" width=\"220\" height=\"168\" \/><\/a><p id=\"caption-attachment-1297\" class=\"wp-caption-text\">\u56f34 75\u00b0C\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u308b<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/EF0654?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">FastAP Thermosensitive Alkaline Phosphatase<\/a>\u306e\u4e0d\u6d3b\u5316\u3002 DNA\u8131\u30ea\u30f3\u9178\u5316\u53cd\u5fdc\u6eb6\u6db2(200 units\/ml phosphatase)\u306f37\u00b0C\u306730\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u300175\u2103\u3067\u4e0d\u6d3b\u5316\u306e\u51e6\u7406\u3092\u884c\u3044\u3001\u30b0\u30e9\u30d5\u306b\u793a\u3057\u305f\u6642\u9593\u3054\u3068\u306bp-NPP\u30a2\u30c3\u30bb\u30a4\u306b\u3088\u3063\u3066\u6b8b\u5b58\u3059\u308bphosphatase\u6d3b\u6027\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002<\/p><\/div>\n<h2>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3<\/h2>\n<p>\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u3068\u30d9\u30af\u30bf\u30fc\u3092\u9023\u7d50\u3059\u308b\u306b\u306f\u30ea\u30ac\u30fc\u30bc\u306b\u3088\u308b\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002T4 DNA Ligase\u3092\u7528\u3044\u305f\u5834\u5408\u3001\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u53cd\u5fdc\u306b\u306f1\u6642\u9593\u304b\u3089\u4e00\u663c\u591c\u8981\u3057\u307e\u3059\u3002\u305d\u308c\u306b\u5bfe\u3057\u3001<strong><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K1422?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Rapid DNA Ligation Kit<\/a>\u306f\u3001T4 DNA ligase\u3092\u7528\u3044\u30661\u6642\u9593\u53cd\u5fdc\u3057\u305f\u3068\u304d\u3068\u540c\u7b49\u306e\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u53cd\u5fdc\u52b9\u7387\u3092\u5ba4\u6e295\u5206\u9593\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3067\u5b9f\u73fe\u3057\u307e\u3059<\/strong>\uff08\u56f35\uff09\u3002\u52a0\u3048\u3066\u3001\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u53cd\u5fdc\u6db2\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K2710?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">TransformAid Bacterial Transformation Kit<\/a>\u307e\u305f\u306f\u4ed6\u306e\u624b\u6cd5\u306b\u3088\u3063\u3066\u4f5c\u88fd\u3057\u305f\u5927\u8178\u83cc\u306e\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u3092\u7528\u3044\u305f\u5f62\u8cea\u8ee2\u63db\u306b\u76f4\u63a5\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<div id=\"attachment_1298\" style=\"width: 273px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig5.jpg\" rel=\"attachment wp-att-1298\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1298\" class=\"size-full wp-image-1298\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/fast-cloning-fig5.jpg\" alt=\"fast-cloning-fig5\" width=\"263\" height=\"180\" \/><\/a><p id=\"caption-attachment-1298\" class=\"wp-caption-text\">\u56f35 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K1422?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Rapid DNA Ligation Kit<\/a>\u306b\u3088\u308b\u7a81\u51fa\u672b\u7aef\u3068\u5e73\u6ed1\u672b\u7aef\u306e\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u3002 \u7a81\u51fa\u672b\u7aef\u306e\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u306fLambda DNA\u306e2.1 kb <em>Pst<\/em>I\u65ad\u7247\u3092<em>Pst<\/em>I\u3067\u5207\u65ad\u5f8c\u306b\u8131\u30ea\u30f3\u9178\u5316\u51e6\u7406\u3057\u305fpUC19 DNA \u306e\u4e2d\u306b\u633f\u5165\u3059\u308b\u3053\u3068\u3067\u884c\u3044\u307e\u3057\u305f\u3002\u5e73\u6ed1\u672b\u7aef\u306e\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u306fLambda DNA \u306e2.3 kb <em>Pvu<\/em>II\u65ad\u7247\u3092<em>Sma<\/em>I\u3067\u5207\u65ad\u5f8c\u306b\u8131\u30ea\u30f3\u9178\u5316\u51e6\u7406\u3057\u305fpUC19\u306e\u4e2d\u306b\u633f\u5165\u3059\u308b\u3053\u3068\u3067\u884c\u3044\u307e\u3057\u305f\u3002\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u52b9\u7387\u306f\u5927\u8178\u83ccXL1-Blue\u682a\u306e\u5f62\u8cea\u8ee2\u63db\u306b\u3088\u3063\u3066\u6c7a\u5b9a\u3057\u307e\u3057\u305f\u3002<\/p><\/div>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>1.5 kb\u306e\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u3092PCR\u3067\u6e96\u5099\u3057\u305f\u5834\u5408\u3001<strong>\u5f93\u6765\u306eStandard Cloning\u88fd\u54c1\u3068Fast cloning\u88fd\u54c1\u3092\u7528\u3044\u305fPCR\u5897\u5e45\u304b\u3089\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u53cd\u5fdc\u307e\u3067\u306e\u6642\u9593\u3092\u6bd4\u8f03\u3059\u308b\u3068\u3001\u524d\u8005\u3067\u306f\u6700\u77ed\u3067\u30825.5\u6642\u9593\u8981\u3057\u307e\u3059\u304c\u3001\u5f8c\u8005\u3067\u306f\u6700\u77ed1.5\u6642\u9593\u306b\u77ed\u7e2e\u3067\u304d\u307e\u3059<\/strong>\uff08\u88681\uff09\u3002<\/p>\n<table>\n<caption>\u88681 Standard Cloning\u3068Fast Cloning\u306e\u6bd4\u8f03<\/caption>\n<tbody>\n<tr>\n<td><\/td>\n<td>Standard Cloning\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/td>\n<td>Fast Cloning\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/td>\n<\/tr>\n<tr>\n<td>1.5 kb DNA\u65ad\u7247\u306e\u5897\u5e45<\/td>\n<td><em>Pfu<\/em>\u00a0Polymerase<br \/>\nFidelity 8x\u00a0<em>Taq<\/em><br \/>\n1\u6642\u9593<\/td>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/F530S?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Phusion DNA Polymerase<\/a><br \/>\nFidelity 52x\u00a0<em>Taq<\/em><br \/>\n20\u5206<\/td>\n<\/tr>\n<tr>\n<td>\u96fb\u6c17\u6cf3\u52d5<\/td>\n<td>1\u6642\u9593<\/td>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/SM1331?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener\">GeneRuler DNA ladders<\/a><br \/>\n45\u5206<\/td>\n<\/tr>\n<tr>\n<td>\u5236\u9650\u9175\u7d20\u51e6\u7406<\/td>\n<td>1-16\u6642\u9593<\/td>\n<td><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/thermo-scientific-restriction-modifying-enzymes\/restriction-enzymes-thermo-scientific\/fastdigest-thermo-scientific.html?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener\">FastDigest enzymes<\/a><br \/>\n5-15\u5206<\/td>\n<\/tr>\n<tr>\n<td>\u30d9\u30af\u30bf\u30fc\u306e\u8131\u30ea\u30f3\u9178\u5316\u3068AP\u306e\u4e0d\u6d3b\u6027\u5316<\/td>\n<td>CIAP<br \/>\n1.5\u6642\u9593<\/td>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/EF0654?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">FastAP Alkaline Phosphatase<\/a><br \/>\n15\u5206<\/td>\n<\/tr>\n<tr>\n<td>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3<\/td>\n<td>T4 DNA Ligase<br \/>\n1-16\u6642\u9593<\/td>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K1422?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Rapid DNA Ligation Kit<\/a><br \/>\n5\u5206<\/td>\n<\/tr>\n<tr>\n<td><strong>\u5408\u8a08<\/strong><\/td>\n<td><span style=\"font-size: 14pt\"><strong>5.5 \u6642\u9593\u4ee5\u4e0a<\/strong><\/span><\/td>\n<td><span style=\"font-size: 18pt\"><strong><span class=\"red\"><span class=\"big\">1.5 \u6642\u9593<\/span><\/span><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u5f8c\u306e\u30b9\u30c6\u30c3\u30d7\u306f\u3053\u3061\u3089<\/h2>\n<h4 class=\"single-title\"><span class=\"no-wow\">\u5927\u8178\u83cc\u306e\u5f62\u8cea\u8ee2\u63db\u306b\u3064\u3044\u3066<\/span><\/h4>\n<p>\u8a73\u3057\u304f\u306f<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/troubleshooting-your-transformations\/?cid=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3053\u3061\u3089<\/a>\u306e\u8a18\u4e8b\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<h4 class=\"single-title\">\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u3092\u6301\u3063\u305f\u7d44\u63db\u3048\u4f53\u3092\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u65b9\u6cd5\u306b\u3064\u3044\u3066<\/h4>\n<p>\u8a73\u3057\u304f\u306f<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/5-ways-to-screen-for-recombinant-clones\/?cid=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3053\u3061\u3089<\/a>\u306e\u8a18\u4e8b\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>\u3053\u308c\u3060\u3051\u306f\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u57fa\u790e\u77e5\u8b58\u3001\u30b3\u30b3\u306b\u3042\u308a\u307e\u3059\uff01<\/h2>\n<p>Invitrogen \u5206\u5b50\u751f\u7269\u5b66\u6559\u5ba4\u3067\u306f\u3001\u5206\u5b50\u751f\u7269\u5b66\u306e\u6559\u80b2\u306e\u30e1\u30a4\u30f3\u3068\u3057\u3066\u3001\u521d\u5fc3\u8005\u3060\u3051\u3067\u306a\u304f\u7d4c\u9a13\u306e\u3042\u308b\u5206\u5b50\u751f\u7269\u5b66\u8005\u306e\u305f\u3081\u306b\u3001\u8c4a\u5bcc\u3067\u4fe1\u983c\u3067\u304d\u308b\u6280\u8853\u7684\u306a\u5185\u5bb9\u3092\u3054\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u57fa\u790e\u77e5\u8b58\u3092\u5b66\u3076\u6642\u306b\u306f\u3044\u3064\u3067\u3082\u3001Invitrogen \u5206\u5b50\u751f\u7269\u5b66\u6559\u5ba4\u306e\u7121\u6599PDF\u3092\u6d3b\u7528\u3057\u3066\u304f\u3060\u3055\u3044\uff01<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/mol-bio-school.html?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u305f\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u89e3\u6790\u3059\u308b\u305f\u3081\u306b\u3001\u30b5\u30d6\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306f\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u3067\u5fc5\u9808\u306a\u6280\u8853\u3067\u3059\u3002\u4e00\u822c\u7684\u306b\u3053\u306e\u5de5\u7a0b\u306f1\u65e5\u4ee5\u4e0a\u8981\u3059\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u3053\u306e\u5de5\u7a0b\u3092\u6700\u77ed\u30671.5\u6642\u9593\u306b\u77ed\u7e2e\u3067\u304d\u308b\u5f0a\u793e\u306e\u88fd\u54c1\u3092\u7528\u3044\u305fFast 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