{"id":457,"date":"2020-03-04T00:00:00","date_gmt":"2020-03-03T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-assay-compatibility\/"},"modified":"2025-02-28T11:34:09","modified_gmt":"2025-02-28T02:34:09","slug":"protein-assay-compatibility","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-assay-compatibility\/","title":{"rendered":"\u3042\u306a\u305f\u306e\u30b5\u30f3\u30d7\u30eb\u306f\u5927\u4e08\u592b\uff1f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u306e\u9069\u5408\u6027\u5224\u5b9a\u65b9\u6cd5"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>\u5f0a\u793e\u3067\u306f\u3055\u307e\u3056\u307e\u306a\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u7528\u8a66\u85ac\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u304c\u3001<strong>\u3069\u306e\u5b9a\u91cf\u6cd5\u306b\u304a\u3044\u3066\u3082\u826f\u304f\u304a\u554f\u3044\u5408\u308f\u305b\u3044\u305f\u3060\u304f\u306e\u304c\u3001\u300c\u81ea\u5206\u306e\u30b5\u30f3\u30d7\u30eb\u306f\u6e2c\u5b9a\u3067\u304d\u308b\u304b\uff1f\u300d\u3068\u3044\u3046\u3054\u8cea\u554f\u3067\u3059<\/strong>\u3002\u4eca\u56de\u306f<strong>\u5404\u5b9a\u91cf\u6cd5\u306b\u304a\u3051\u308b\u5171\u5b58\u8a31\u5bb9\u6210\u5206\u306e\u60c5\u5831\u3084\u8907\u6570\u306e\u6210\u5206\u304c\u5171\u5b58\u3057\u3066\u3044\u308b\u5834\u5408\u306e\u9069\u5408\u6027\u5224\u5b9a\u65b9\u6cd5<\/strong>\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>\u5206\u5149\u5149\u5ea6\u8a08\u3092\u7528\u3044\u305f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u306b\u306f\u3001\u3055\u307e\u3056\u307e\u306a\u6e2c\u5b9a\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002\u6e2c\u5b9a\u30b5\u30f3\u30d7\u30eb\u306b\u9069\u3057\u305f\u6e2c\u5b9a\u6cd5\u3092\u9078\u629e\u3059\u308b\u306b\u306f\u3001\u5404\u5b9a\u91cf\u6cd5\u306e\u7279\u5fb4\u3092\u77e5\u3063\u3066\u304a\u304f\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u5f0a\u793e\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u8a66\u85ac\u306b\u95a2\u3059\u308b\u3001\u7279\u5fb4\u3084\u9078\u629e\u65b9\u6cd5\u306b\u3064\u3044\u3066\u306f\u3001\u4ee5\u524d\u306e\u8a18\u4e8b\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic3\/?CID=Social_LAB\" target=\"_blank\" rel=\"noopener\">\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u7a2e\u985e\u3092\u307e\u3068\u3081\u3066\u307f\u305f\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c3\u56de \u00bb<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/?CID=Social_LAB\" target=\"_blank\" rel=\"noopener\">BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de \u00bb<\/a><\/p>\n<h2>\u5171\u5b58\u8a31\u5bb9\u6210\u5206\u3068\u6fc3\u5ea6<\/h2>\n<p>\u5f0a\u793e\u3067\u306f\u3001<strong>\u751f\u5316\u5b66\u5b9f\u9a13\u3067\u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u308b\u6210\u5206\u3084\u30d0\u30c3\u30d5\u30a1\u30fc\u985e\u306e\u9069\u5408\u6fc3\u5ea6\u306b\u3064\u3044\u3066\u3001\u5f0a\u793e\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u8a66\u85ac\u6bce\u306b\u307e\u3068\u3081\u305f\u8868\u3092\u516c\u958b\u3057\u3066\u3044\u307e\u3059<\/strong>\u3002\u4e0b\u8a18\u306e\u753b\u50cf\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u8a73\u7d30\u3092\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\uff082\u30da\u30fc\u30b8\u76ee\u4ee5\u964d\u306b\u8868\u304c\u3042\u308a\u307e\u3059\uff09\u3002<\/p>\n<div id=\"attachment_1312\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/tools.thermofisher.com\/content\/sfs\/brochures\/TR0068-Protein-assay-compatibility.pdf?CID=Social_LAB\" rel=\"attachment wp-att-1312\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1312\" class=\"wp-image-1312 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-assay-compatibility-fig1.jpg\" alt=\"protein-assay-compatibility-fig1\" width=\"500\" height=\"241\" \/><\/a><p id=\"caption-attachment-1312\" class=\"wp-caption-text\"><a href=\"https:\/\/tools.thermofisher.com\/content\/sfs\/brochures\/TR0068-Protein-assay-compatibility.pdf?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa35_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Protein assay compatibility table<\/a> (\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068PDF\u30d5\u30a1\u30a4\u30eb\u304c\u958b\u304d\u307e\u3059)<\/p><\/div>\n<p>\u8868\u4e2d\u306e\u5de6\u7aef\u306e\u5217\u306b\u306f\u5404\u6210\u5206\u540d\u3092\u3001\u53f3\u5074\u306e\u5217\u306b\u306f\u5404\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306b\u304a\u3051\u308b\u5171\u5b58\u8a31\u5bb9\u6fc3\u5ea6\u3092\u8a18\u8f09\u3057\u3066\u3044\u307e\u3059\u3002\u4f8b\u3048\u3070\u30012-Mercaptoethanol\u306f\u3001660 nm\uff08<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/22660?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa35_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce 660 nm Protein Assay<\/a>\uff09\u306e\u5834\u5408\u30011M\u307e\u3067\u5171\u5b58\u3057\u3066\u3082\u6e2c\u5b9a\u306f\u963b\u5bb3\u3055\u308c\u307e\u305b\u3093\u304c\u3001BCA\uff08<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-biology\/protein-assays-analysis\/protein-assays\/bca-protein-assays.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa35_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">BCA Protein Assay<\/a>\uff09\u306e\u5834\u5408\u30010.01%\u3088\u308a\u9ad8\u3044\u6fc3\u5ea6\u3067\u5171\u5b58\u3059\u308b\u3068\u6e2c\u5b9a\u304c\u963b\u5bb3\u3055\u308c\u307e\u3059\u3002\u307e\u305f\u30012\u30da\u30fc\u30b8\u76ee\u4e00\u756a\u4e0b\u306b\u8a18\u8f09\u306e<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/78503?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa35_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">M-PER\u2122 Mammalian Protein Extraction Reagent<\/a>\uff08\u54fa\u4e73\u985e\u57f9\u990a\u7d30\u80de\u304b\u3089\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u8a66\u85ac\uff09\u306f\u3001660 nm Protein Assay\u306e\u5834\u5408\u30012\u500d\u5e0c\u91c8\u3059\u308c\u3070\u6e2c\u5b9a\u306f\u963b\u5bb3\u3055\u308c\u305a\u3001BCA Protein Assay\u306e\u5834\u5408\u3001\u5e0c\u91c8\u4e0d\u8981\u3067\u76f4\u63a5\u6e2c\u5b9a\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\u306a\u304a\u3001\u8868\u4e2d\u306e\u6fc3\u5ea6\u306f\u3001\u5404\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306b\u304a\u3051\u308btest tube\u6cd5\u3092\u7528\u3044\u3066\u5f0a\u793e\u3067\u30c6\u30b9\u30c8\u3057\u305f\u6700\u5927\u306e\u5171\u5b58\u8a31\u5bb9\u6fc3\u5ea6\u3067\u3059\u304c\u3001\u3042\u304f\u307e\u3067\u5de6\u7aef\u306e\u5404\u6210\u5206\u304c\u5358\u72ec\u3067\u5b58\u5728\u3059\u308b\u5834\u5408\u306e\u8a31\u5bb9\u6fc3\u5ea6\u3067\u3059\u3002\u5225\u306e\u6210\u5206\u304c\u6df7\u5408\u3055\u308c\u308b\u3068\u3001\u8a31\u5bb9\u6fc3\u5ea6\u304c\u8868\u4e2d\u306e\u5024\u3088\u308a\u3082\u4e0b\u304c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>\u8907\u6570\u306e\u6210\u5206\u304c\u5171\u5b58\u3057\u3066\u3044\u3066\u9069\u5408\u6027\u304c\u4e0d\u660e\u306a\u5834\u5408<\/h2>\n<p>\u30bf\u30f3\u30d1\u30af\u8cea\u6eb6\u6db2\u4e2d\u306b\u8907\u6570\u306e\u6210\u5206\u304c\u5171\u5b58\u3057\u3066\u3044\u308b\u5834\u5408\u3001\u5171\u5b58\u8a31\u5bb9\u6fc3\u5ea6\u306e\u4e00\u89a7\u8868\u304b\u3089\u3067\u306f\u9069\u5408\u6027\u3092\u4e88\u60f3\u3059\u308b\u3053\u3068\u304c\u56f0\u96e3\u3067\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u5834\u5408\u306b\u306f\u3001<strong>\u30b5\u30f3\u30d7\u30eb\u6e2c\u5b9a\u524d\u306b\u3001\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3057\u3066\u9069\u5408\u6027\u3092\u78ba\u8a8d<\/strong>\u3057\u307e\u3059\u3002\u5177\u4f53\u7684\u306b\u306f\u6b21\u306e\u624b\u9806\u3067\u78ba\u8a8d\u3057\u307e\u3059\u3002<\/p>\n<ol>\n<li>\u6c34\u307e\u305f\u306fPBS\u306a\u3069\u6e2c\u5b9a\u3092\u963b\u5bb3\u3057\u306a\u3044\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\uff08BSA\u306a\u3069\uff09\u3092\u5e0c\u91c8\u3057\u3066\u691c\u91cf\u7dda\u3092\u4f5c\u6210<\/li>\n<li>\u30b5\u30f3\u30d7\u30eb\u3068\u540c\u3058\u7d44\u6210\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u542b\u307e\u306a\u3044\u6eb6\u6db2\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u6eb6\u3051\u3066\u3044\u308b\u6eb6\u5a92\uff09\u3092\u6e96\u5099<\/li>\n<li>1\u306e\u6eb6\u6db2\u3067\u691c\u91cf\u7dda\u3092\u4f5c\u6210<\/li>\n<li>1\u30683\u3067\u4f5c\u6210\u3057\u305f\u691c\u91cf\u7dda\u3092\u6bd4\u8f03<\/li>\n<\/ol>\n<p>\u691c\u91cf\u7dda\u6bd4\u8f03\u3092\u884c\u3063\u305f\u3068\u304d\u3001\u6fc3\u5ea6\u306e\u8aa4\u5dee\u304c10%\u4ee5\u5185\u3067\u3042\u308c\u30702\u306e\u6eb6\u6db2\u304c\u9069\u5408\u3059\u308b\u3068\u5224\u65ad\u3057\u307e\u3059\u3002\u8aa4\u5dee\u304c10%\u3092\u8d85\u3048\u308b\u5834\u5408\u306f\u30012\u306e\u6eb6\u6db2\u3092\u4f55\u500d\u304b\u306b\u5e0c\u91c8\u3057\u3001\u5e0c\u91c8\u6eb6\u5a92\u30673\u30684\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n<div id=\"attachment_1314\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-assay-compatibility-fig2.jpg\" rel=\"attachment wp-att-1314\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1314\" class=\"size-full wp-image-1314\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-assay-compatibility-fig2.jpg\" alt=\"protein-assay-compatibility-fig2\" width=\"500\" height=\"232\" \/><\/a><p id=\"caption-attachment-1314\" class=\"wp-caption-text\">\u56f3 \u691c\u91cf\u7dda\u306e\u6bd4\u8f03<\/p><\/div>\n<p>\u4f8b\u3048\u3070\u3001PBS\u3067\u4f5c\u6210\u3057\u305f\u691c\u91cf\u7dda\uff08\u56f3\u4e2d\u306e\u9752\u8272\u5b9f\u7dda\uff09\u3067\u306f\u5438\u5149\u5ea6\u304c1.4\u306e\u3068\u304d1,000 \u00b5g\/mL\u3067\u3059\u304c\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u6eb6\u3051\u3066\u3044\u308b\u6eb6\u5a92\u3067\u4f5c\u6210\u3057\u305f\u691c\u91cf\u7dda\uff08\u56f3\u4e2d\u306e\u7dd1\u8272\u70b9\u7dda\uff09\u3092\u898b\u308b\u3068\u30011,460 \u00b5g\/mL\u3068\u5927\u304d\u304f\u305a\u308c\u3066\u304a\u308a\u3001\u6e2c\u5b9a\u304c\u963b\u5bb3\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u6eb6\u3051\u3066\u3044\u308b\u6eb6\u5a92\u3092\u6c34\u306710\u500d\u5e0c\u91c8\u3057\u3001\u305d\u306e\u6eb6\u6db2\u3067\u4f5c\u6210\u3057\u305f\u691c\u91cf\u7dda\uff08\u56f3\u4e2d\u306e\u6a59\u8272\u70b9\u7dda\uff09\u3092\u898b\u308b\u30681,020 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