{"id":463,"date":"2020-03-04T00:00:00","date_gmt":"2020-03-03T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/remove-detergent-from-protein-samples\/"},"modified":"2025-01-21T16:16:57","modified_gmt":"2025-01-21T07:16:57","slug":"remove-detergent-from-protein-samples","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/remove-detergent-from-protein-samples\/","title":{"rendered":"\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u9ad8\u6fc3\u5ea6\u306e\u754c\u9762\u6d3b\u6027\u5264\u3092\u52b9\u7387\u3088\u304f\u8fc5\u901f\u306b\u9664\u53bb\u3059\u308b\u65b9\u6cd5"},"content":{"rendered":"<p>\u7d30\u80de\u3084\u7d44\u7e54\u304b\u3089\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u3092\u8abf\u88fd\u3059\u308b\u3068\u304d\u3001<strong>\u758e\u6c34\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u53ef\u6eb6\u5316\u3059\u308b\u76ee\u7684\u3067\u754c\u9762\u6d3b\u6027\u5264\u304c\u7528\u3044\u3089\u308c\u307e\u3059\u304c\u3001\u7d9a\u3044\u3066\u884c\u308f\u308c\u308b\u4e0b\u6d41\u306e\u5b9f\u9a13\u306b\u3088\u3063\u3066\u306f\u754c\u9762\u6d3b\u6027\u5264\u304c\u963b\u5bb3\u56e0\u5b50\u3068\u306a\u308b\u305f\u3081\u3001\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u9664\u53bb\u3057\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059<\/strong>\u3002\u4f8b\u3048\u3070\u3001\u754c\u9762\u6d3b\u6027\u5264\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6d3b\u6027\u3092\u963b\u5bb3\u3057\u3066\u3057\u307e\u3046\u5834\u5408\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u3084\u96fb\u6c17\u6cf3\u52d5\u306b\u9069\u5408\u3057\u306a\u3044\u5834\u5408\u306a\u3069\u3067\u3059\u3002\u754c\u9762\u6d3b\u6027\u5264\u3092\u9664\u304f\u65b9\u6cd5\u3068\u3057\u3066\u306f\u3001\u900f\u6790\u3084\u9650\u5916\u308d\u904e\u306b\u3088\u308a\u4f4e\u5206\u5b50\u3092\u9664\u53bb\u3059\u308b\u65b9\u6cd5\u3001\u3042\u308b\u3044\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6c88\u6bbf\u3055\u305b\u3001\u518d\u6eb6\u89e3\u3059\u308b\u65b9\u6cd5\u304c\u3042\u3052\u3089\u308c\u307e\u3059\u3002<\/p>\n<p>\u754c\u9762\u6d3b\u6027\u5264\u306f\u3001\u81e8\u754c\u30df\u30bb\u30eb\u6fc3\u5ea6\uff08Critical Micelle Concentration: CMC\uff09\u3092\u8d85\u3048\u308b\u3068\u30df\u30bb\u30eb\u3092\u5f62\u6210\u3059\u308b\u6027\u8cea\u3092\u3082\u3061\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u53ef\u6eb6\u5316\u3067\u306fCMC\u4ee5\u4e0a\u306e\u6fc3\u5ea6\u3067\u4f7f\u7528\u3057\u307e\u3059\u3002\u900f\u6790\u3084\u9650\u5916\u308d\u904e\u306f\u3001CMC\u304c\u9ad8\u3044\u754c\u9762\u6d3b\u6027\u5264\u3084\u30df\u30bb\u30eb\u4f1a\u5408\u6570\u304c\u5c0f\u3055\u3044\u754c\u9762\u6d3b\u6027\u5264\u306e\u9664\u53bb\u306b\u306f\u6709\u7528\u3067\u3059\u304c\u3001CMC\u304c\u4f4e\u3044\u5834\u5408\u3084\u30df\u30bb\u30eb\u4f1a\u5408\u6570\u304c\u5927\u304d\u3044\u5834\u5408\u3001<strong>\u307b\u3068\u3093\u3069\u306e\u754c\u9762\u6d3b\u6027\u5264\u304c\u900f\u6790\u819c\u306e\u30dd\u30a2\u30b5\u30a4\u30ba\u3088\u308a\u3082\u5927\u304d\u3044\u30df\u30bb\u30eb\u72b6\u614b\u3067\u5b58\u5728\u3057\u3066\u304a\u308a\u3001\u52b9\u7387\u3088\u304f\u9664\u53bb\u3059\u308b\u3053\u3068\u306f\u56f0\u96e3<\/strong>\u3067\u3059\u3002\u307e\u305f<strong>\u30bf\u30f3\u30d1\u30af\u8cea\u6c88\u6bbf\u6cd5\u3082\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5909\u6027\u3059\u308b\u3001\u3042\u308b\u3044\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u518d\u6eb6\u89e3\u304c\u3067\u304d\u306a\u304f\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059<\/strong>\u3002<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/87777?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa38_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce\u2122 Detergent Removal Spin Columns<\/a>\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u6eb6\u6db2\u3084\u30da\u30d7\u30c1\u30c9\u6eb6\u6db2\u304b\u3089SDS, CHAPS, Triton X-100, NP-40\u306a\u3069\u306e\u754c\u9762\u6d3b\u6027\u5264\uff081-5%\uff09\u3092<strong>95%\u4ee5\u4e0a\u306e\u52b9\u7387\u3067\u3001\u8fc5\u901f\u306b\u9664\u53bb\u3059\u308b\u305f\u3081\u306e\u30b9\u30d4\u30f3\u30ab\u30e9\u30e0<\/strong>\u3067\u3059\u3002\u30b9\u30d4\u30f3\u30ab\u30e9\u30e0\u306b\u5145\u586b\u6e08\u307f\u306e\u30ec\u30b8\u30f3\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u3084\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u3067\u4f7f\u7528\u3055\u308c\u308b\u4e00\u822c\u7684\u306a\u754c\u9762\u6d3b\u6027\u5264\u3068\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u7d50\u5408\u3057\u307e\u3059\u3002\u64cd\u4f5c\u306f\u30ec\u30b8\u30f3\u306e\u5e73\u8861\u5316\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u6dfb\u52a0\u30fb\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u30fb\u56de\u53ce\u3068\u7c21\u4fbf\u3067\u300110\u5206\u4ee5\u5185\u306b\u754c\u9762\u6d3b\u6027\u5264\u306e\u9664\u53bb\u304c\u5b8c\u4e86\u3057\u307e\u3059\u3002\u900f\u6790\u3084\u9650\u5916\u308d\u904e\u306b\u304a\u3044\u3066\u61f8\u5ff5\u3055\u308c\u308b\u3001<strong>\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u30da\u30d7\u30c1\u30c9\u306e\u975e\u7279\u7570\u7684\u5438\u7740\u306b\u3088\u308b\u30ed\u30b9\u306f\u5c11\u91cf\u3067\u3059<\/strong>\uff08\u88681\u53c2\u7167\uff09\u3002\u307e\u305f\u3001\u4f4e\u6fc3\u5ea6\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u6eb6\u6db2\u304b\u3089\u754c\u9762\u6d3b\u6027\u5264\u3092\u9664\u53bb\u3057\u305f\u3044\u5834\u5408\u306b\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/88305?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa38_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">HiPPR\u2122 Detergent Removal Spin Column Kit<\/a>\u304c\u6709\u52b9\u3067\u3059\u3002<strong>1-100 \u03bcg\/mL\u306e\u4f4e\u6fc3\u5ea6\u30bf\u30f3\u30d1\u30af\u8cea\u6eb6\u6db2\u304b\u3089\u3001\u754c\u9762\u6d3b\u6027\u5264\uff080.5-1%\uff09\u309295%\u4ee5\u4e0a\u9664\u53bb\u3067\u304d\u307e\u3059<\/strong>\u3002<\/p>\n<p>\u4e0b\u8a18\u306e\u8868\u3067\u306f\u3001\u754c\u9762\u6d3b\u6027\u5264\u3092\u542b\u3080BSA\uff080.1 mL, 100 \u03bcg\uff09\u3092<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/87777?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa38_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce\u2122 Detergent Removal Spin Columns<\/a>\u3067\u51e6\u7406\u3057\u3001\u754c\u9762\u6d3b\u6027\u5264\u306e\u9664\u53bb\u7387\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u56de\u53ce\u7387\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002BSA\u4ee5\u5916\u3067\u3082\u3001insulin\uff085.7 kDa\uff09\u3001\u03b1-lactalbumin\uff0814.2 kDa\uff09\u3001carbonic anhydrase\uff0829 kDa\uff09\u3067\u305d\u308c\u305e\u308c\u540c\u69d8\u306e\u7d50\u679c\u304c\u5f97\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<table>\n<caption>\u8868 \u5404\u754c\u9762\u6d3b\u6027\u5264\u306e\u9664\u53bb\u7387\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u56de\u53ce\u7387<\/caption>\n<tbody>\n<tr>\n<td>\u754c\u9762\u6d3b\u6027\u5264<\/td>\n<td>\u754c\u9762\u6d3b\u6027\u5264\u9664\u53bb\u7387 (%)<\/td>\n<td>BSA \u56de\u53ce\u7387 (%)<\/td>\n<\/tr>\n<tr>\n<td>SDS, 2.5%<\/td>\n<td>99<\/td>\n<td>95<\/td>\n<\/tr>\n<tr>\n<td>CHAPS, 3%<\/td>\n<td>99<\/td>\n<td>90<\/td>\n<\/tr>\n<tr>\n<td>Triton X-100, 2%<\/td>\n<td>99<\/td>\n<td>87<\/td>\n<\/tr>\n<tr>\n<td>NP-40, 1%<\/td>\n<td>95<\/td>\n<td>91<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 8pt\">\u203b\u305d\u306e\u4ed6\u306e\u754c\u9762\u6d3b\u6027\u5264\u306e\u9664\u53bb\u7387\u306b\u3064\u304d\u307e\u3057\u3066\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/87777?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa38_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce\u2122 Detergent Removal Spin Columns<\/a>\u306e\u88fd\u54c1\u60c5\u5831\u30da\u30fc\u30b8\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<h2>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa38_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u76ee\u7684\u304a\u3088\u3073\u305d\u306e\u624b\u7d9a\u304d\u4e0a\u3067\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7d30\u80de\u3084\u7d44\u7e54\u304b\u3089\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u3092\u8abf\u88fd\u3059\u308b\u3068\u304d\u3001\u758e\u6c34\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u53ef\u6eb6\u5316\u3059\u308b\u76ee\u7684\u3067\u754c\u9762\u6d3b\u6027\u5264\u304c\u7528\u3044\u3089\u308c\u307e\u3059\u304c\u3001\u7d9a\u3044\u3066\u884c\u308f\u308c\u308b\u4e0b\u6d41\u306e\u5b9f\u9a13\u306b\u3088\u3063\u3066\u306f\u754c\u9762\u6d3b\u6027\u5264\u304c\u963b\u5bb3\u56e0\u5b50\u3068\u306a\u308b\u305f\u3081\u3001\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u9664\u53bb\u3057\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u754c\u9762\u6d3b\u6027\u5264\u3092\u52b9\u7387\u3088\u304f\u8fc5\u901f\u306b\u9664\u53bb\u3059\u308b\u88fd\u54c1\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":988,"featured_media":2747,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[61,21],"division":[],"class_list":{"0":"post-463","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-protein-purification","9":"tag-protein-analysis","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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