{"id":467,"date":"2020-04-01T00:00:00","date_gmt":"2020-03-31T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/clean-blot-ip-detection-reagent\/"},"modified":"2026-02-20T11:17:56","modified_gmt":"2026-02-20T02:17:56","slug":"clean-blot-ip-detection-reagent","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/clean-blot-ip-detection-reagent\/","title":{"rendered":"\u514d\u75ab\u6c88\u964d\u30a6\u30a7\u30b9\u30bf\u30f3\u691c\u51fa\u3067\u6297\u4f53\u7531\u6765\u306e\u30d0\u30f3\u30c9\u304c\u90aa\u9b54\u306a\u3068\u304d\u306b\u30aa\u30b9\u30b9\u30e1\u306e\u88fd\u54c1\u306f\u3053\u308c\uff01"},"content":{"rendered":"<p>\u514d\u75ab\u6c88\u964d\uff08IP\uff09\u306b\u3088\u308a\u56de\u53ce\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u691c\u51fa\u3059\u308b\u3068\u3001\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u4ee5\u5916\u306b\u514d\u75ab\u6c88\u964d\u306b\u5229\u7528\u3057\u305f\u6297\u4f53\uff08\u4ee5\u4e0bIP\u6297\u4f53\uff09\u306e\u30d0\u30f3\u30c9\u304c\u691c\u51fa\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\uff08\u56f31\u5de6\u5074\u306e2\u3064\u306e\u30d1\u30cd\u30eb\uff09\u3002\u56f31\u306e\u3088\u3046\u306b\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30d0\u30f3\u30c9\u3068IP\u6297\u4f53\u30d0\u30f3\u30c9\u306e\u79fb\u52d5\u5ea6\u304c\u7570\u306a\u308b\u5834\u5408\u306f\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u691c\u51fa\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u304c\u3001<strong>\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3068IP\u6297\u4f53\u306e\u79fb\u52d5\u5ea6\u304c\u8fd1\u3044\u5834\u5408\u3084IP\u6297\u4f53\u306e\u691c\u51fa\u5f37\u5ea6\u304c\u9ad8\u304f\u76ee\u7684\u30d0\u30f3\u30c9\u304c\u30de\u30b9\u30af\u3055\u308c\u3066\u3057\u307e\u3046\u5834\u5408\u306b\u306f\u3001\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u691c\u51fa\u3092\u78ba\u8a8d\u3059\u308b\u3053\u3068\u304c\u56f0\u96e3<\/strong>\u306b\u306a\u308a\u307e\u3059\uff08\u56f32 Lane 6\uff09\u3002<strong>\u3053\u3093\u306a\u3068\u304d\u306b\u304a\u3059\u3059\u3081\u3059\u308b\u306e\u304c<\/strong><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/21230?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Clean-Blot IP Detection Reagent<\/a>\u3067\u3059\u3002<\/p>\n<p><strong><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/21230?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Clean-Blot IP Detection Reagent<\/a>\u306f\u3001\u5909\u6027\u3057\u3066\u3044\u306a\u3044\u6297\u4f53\u3068\u306f\u53cd\u5fdc\u3057\u307e\u3059\u304c\u5909\u6027\u3057\u305f\u6297\u4f53\u3068\u306f\u53cd\u5fdc\u3057\u306a\u3044HRP\u6a19\u8b58\u5206\u5b50<\/strong>\u3067\u3059\u3002\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u304a\u3044\u3066\u3001\u4e8c\u6b21\u6297\u4f53\u306e\u4ee3\u308f\u308a\u306b\u4f7f\u7528\u3059\u308b\u3068\u3001\u4e00\u6b21\u6297\u4f53\u3068\u306f\u53cd\u5fdc\u3057\u307e\u3059\u304c\u96fb\u6c17\u6cf3\u52d5\u6642\u306bSDS\uff0f\u71b1\u5909\u6027\u3057\u305fIP\u6297\u4f53\u3068\u306f\u53cd\u5fdc\u3057\u306a\u3044\u305f\u3081\u3001\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7279\u7570\u7684\u306b\u691c\u51fa\u3067\u304d\u307e\u3059\uff08\u56f31\u304a\u3088\u3073\u56f32\uff09\u3002<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/21230?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Clean-Blot IP Detection Reagent<\/a>\u306f\u3001\u52d5\u7269\u7a2e\u3084\u30a2\u30a4\u30bd\u30bf\u30a4\u30d7\u306e\u7570\u306a\u308b\u5e45\u5e83\u3044\u6297\u4f53\u3068\u53cd\u5fdc\u3067\u304d\u308b\uff08\u88681\u53c2\u7167\uff09\u305f\u3081\u3001\u4e8c\u6b21\u6297\u4f53\u306e\u3088\u3046\u306b\u4e00\u6b21\u6297\u4f53\u306b\u5408\u308f\u305b\u3066\u5909\u66f4\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u305b\u3093\u3002\u3082\u3061\u308d\u3093\u3001\u5316\u5b66\u767a\u5149\u691c\u51fa\u306b\u4f7f\u7528\u3057\u305f\u5834\u5408\u306f<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic6\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\u30fb\u30ea\u30d7\u30ed\u30fc\u30d3\u30f3\u30b0<\/a>\u3092\u884c\u3046\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<div id=\"attachment_1346\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/clean-blot-ip-detection-reagent-fig1.jpg\" rel=\"attachment wp-att-1346\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1346\" class=\"size-full wp-image-1346\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/clean-blot-ip-detection-reagent-fig1.jpg\" alt=\"clean-blot-ip-detection-reagent-fig1\" width=\"500\" height=\"279\" \/><\/a><p id=\"caption-attachment-1346\" class=\"wp-caption-text\">\u56f31 \u4e8c\u6b21\u6297\u4f53\u3092\u7528\u3044\u305f\u4e00\u822c\u7684\u306a\u691c\u51fa\u3068CleanBlot\u3092\u7528\u3044\u305f\u691c\u51fa\u3002\u30de\u30a6\u30b9\u809d\u81d3\u62bd\u51fa\u6db2\u304b\u3089\u514d\u75ab\u6c88\u964d\u306b\u3088\u308aCdk1\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u56de\u53ce\u3057\u3001\u4e00\u6b21\u6297\u4f53\u3068\u3057\u3066\u30de\u30a6\u30b9\u6297Cdk1\u6297\u4f53\u3092\u7528\u3044\u3066\u5316\u5b66\u767a\u5149\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u884c\u3044\u307e\u3057\u305f\uff08\u56f3\u4e2d\u306e\u300c\uff0dPrimary\u300d\u3067\u306f\u4e00\u6b21\u6297\u4f53\u3092\u53cd\u5fdc\u3055\u305b\u305a\u306b\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u884c\u3063\u3066\u3044\u307e\u3059\uff09\u3002\u4e8c\u6b21\u6297\u4f53\u3068\u3057\u3066HRP\u6a19\u8b58\u30e4\u30ae\u6297\u30de\u30a6\u30b9\u6297\u4f53\u3092\u7528\u3044\u305f\u5834\u5408\u3001\u96fb\u6c17\u6cf3\u52d5\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u542b\u307e\u308c\u3066\u3044\u305fIP\u6297\u4f53\u306e\u30d0\u30f3\u30c9\u304c\u691c\u51fa\u3055\u308c\u307e\u3057\u305f\uff08\u5de6\u5074\u306e2\u3064\u306e\u30d1\u30cd\u30eb\uff09\u3002\u4e00\u65b9\u3001\u4e8c\u6b21\u6297\u4f53\u306e\u4ee3\u308f\u308a\u306bClean-Blot IP Detection Reagent\u3092\u7528\u3044\u305f\u5834\u5408\u3001IP\u6297\u4f53\u306f\u691c\u51fa\u3055\u308c\u305a\u3001\u76ee\u7684\u30d0\u30f3\u30c9\u304c\u7279\u7570\u7684\u306b\u691c\u51fa\u3055\u308c\u307e\u3057\u305f\uff08\u5de6\u304b\u30893\u756a\u76ee\u306e\u30d1\u30cd\u30eb\uff09\u3002<\/p><\/div>\n<p>\u00a0<\/p>\n<div id=\"attachment_1347\" style=\"width: 470px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/clean-blot-ip-detection-reagent-fig2.jpg\" rel=\"attachment wp-att-1347\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1347\" class=\"size-full wp-image-1347\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/clean-blot-ip-detection-reagent-fig2.jpg\" alt=\"clean-blot-ip-detection-reagent-fig2\" width=\"460\" height=\"265\" \/><\/a><p id=\"caption-attachment-1347\" class=\"wp-caption-text\">\u56f32\uff0e\u6297\u4f53\u91cd\u5dee\u3068\u5206\u5b50\u91cf\u306e\u8fd1\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u514d\u75ab\u6c88\u964d\u30a2\u30c3\u30bb\u30a4\u3002A431\u7d30\u80de\u304b\u3089\u514d\u75ab\u6c88\u964d\u306b\u3088\u308aP53\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u56de\u53ce\u3057\u3001\u4e00\u6b21\u6297\u4f53\u3068\u3057\u3066\u30de\u30a6\u30b9\u6297P53\u6297\u4f53\u3092\u7528\u3044\u3066\u5316\u5b66\u767a\u5149\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u4e8c\u6b21\u6297\u4f53\u3068\u3057\u3066HRP\u6a19\u8b58\u30e4\u30ae\u6297\u30de\u30a6\u30b9\u6297\u4f53\u3092\u7528\u3044\u305f\u5834\u5408\u3001P53\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30d0\u30f3\u30c9\u304c\u514d\u75ab\u6c88\u964d\u6297\u4f53\u306e\u91cd\u9396\u30d0\u30f3\u30c9\u306b\u30de\u30b9\u30af\u3055\u308c\u307e\u3057\u305f\uff08Lane 6\uff09\u3002\u4e00\u65b9\u3001\u4e8c\u6b21\u6297\u4f53\u306e\u4ee3\u308f\u308a\u306bClean-Blot IP Detection Reagent\u3092\u7528\u3044\u305f\u5834\u5408\u3001P53\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30d0\u30f3\u30c9\u304c\u7279\u7570\u7684\u306b\u691c\u51fa\u3055\u308c\u307e\u3057\u305f\uff08Lane 1, 4, 5\uff09\u3002<br \/>Lane 1: A431\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\uff08\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff09<br \/>Lane 2-3: \u30e9\u30a4\u30bb\u30fc\u30c8\u306a\u3057\u3067\u514d\u75ab\u6c88\u964d\u3057\u305f\u6d17\u6d44\u753b\u5206\u3068\u6eb6\u51fa\u753b\u5206\uff08\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff09<br \/>Lane 4-6: A431\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u3092\u514d\u75ab\u6c88\u964d\u51e6\u7406\u3057\u305f\u6d17\u6d44\u753b\u5206\u3068\u6eb6\u51fa\u753b\u5206<\/p><\/div>\n<table><caption>\u8868 Clean-Blot IP Detection Reagent\u3068\u306e\u7d50\u5408\u53cd\u5fdc\u304c\u78ba\u8a8d\u3055\u308c\u3066\u3044\u308b\u6297\u4f53<\/caption>\n<tbody>\n<tr>\n<td>\u52d5\u7269\u7a2e<\/td>\n<td>\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53<\/td>\n<td>\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\uff08\u30a2\u30a4\u30bd\u30bf\u30a4\u30d7\uff09<\/td>\n<\/tr>\n<tr>\n<td>Bovine<\/td>\n<td>Yes<\/td>\n<td>IgG2<\/td>\n<\/tr>\n<tr>\n<td>Goat<\/td>\n<td>Yes<\/td>\n<td>IgG2<\/td>\n<\/tr>\n<tr>\n<td>Human<\/td>\n<td>Yes<\/td>\n<td>IgG1, IgG2, IgG4<\/td>\n<\/tr>\n<tr>\n<td>Mouse<\/td>\n<td>Yes<\/td>\n<td>IgG2a, IgG2b, IgG3<\/td>\n<\/tr>\n<tr>\n<td>Rabbit<\/td>\n<td>Yes<\/td>\n<td>N\/A<\/td>\n<\/tr>\n<tr>\n<td>Rat<\/td>\n<td>Yes<\/td>\n<td>IgG2c<\/td>\n<\/tr>\n<tr>\n<td>Sheep<\/td>\n<td>Yes<\/td>\n<td>IgG2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 8pt\">\u203b\u8868\u4e2d\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u306a\u3044\u6297\u4f53\u306f\u8a55\u4fa1\u3055\u308c\u3066\u3044\u306a\u3044\u304b\u3001\u7d50\u5408\u3057\u306a\u3044\u307e\u305f\u306f\u7d50\u5408\u304c\u5f31\u3044\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\uff08\u8868\u4e2d\u306eN\/A\u306f\u8a55\u4fa1\u3055\u308c\u3066\u3044\u306a\u3044\u3053\u3068\u3092\u793a\u3057\u307e\u3059\uff09\u3002<\/span><\/p>\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9-\u67b6\u6a4b\u5264\u30cf\u30f3\u30c9\u30d6\u30c3\u30af-\u65e5\u672c\u8a9e\u7248\">\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u67b6\u6a4b\u5264\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\uff08\u65e5\u672c\u8a9e\u7248\uff09<\/h2>\n\n\n\n<p>\u5206\u5b50\u540c\u58eb\u3092\u3064\u306a\u3050\u300c\u67b6\u6a4b\u53cd\u5fdc\u300d\u306f\u3001\u5275\u85ac\u5206\u91ce\u3084\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u306b\u304a\u3044\u3066\u3001\u3055\u307e\u3056\u307e\u306a\u76ee\u7684\u3067\u7528\u3044\u3089\u308c\u308b\u6280\u8853\u3067\u3059\u3002\u672c\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u306f\u3001\u67b6\u6a4b\u53cd\u5fdc\u306e\u57fa\u790e\u304b\u3089\u3001\u76ee\u7684\u306b\u5fdc\u3058\u305f\u67b6\u6a4b\u5264\u306e\u9078\u3073\u65b9\u307e\u3067\u3092\u5206\u304b\u308a\u3084\u3059\u304f\u89e3\u8aac\u3057\u3066\u3044\u307e\u3059\u3002\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u306ePDF\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<br><br><a class=\"btn btn-red\" href=\"http:\/\/thermofisher.com\/jp\/ja\/home\/global\/forms\/crosslinker-handbook.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa7_Social_LAB\" target=\"_blank\" rel=\"noreferrer 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href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa7_Social_LAB\" target=\"_blank\" 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