{"id":479,"date":"2016-08-30T00:00:00","date_gmt":"2016-08-30T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/troubleshooting-your-transformations\/"},"modified":"2025-11-28T17:46:20","modified_gmt":"2025-11-28T08:46:20","slug":"troubleshooting-your-transformations","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/troubleshooting-your-transformations\/","title":{"rendered":"\u5927\u8178\u83cc\u306e\u5f62\u8cea\u8ee2\u63db\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u3068\u305d\u306e\u5bfe\u7b56"},"content":{"rendered":"\n<p class=\"has-small-font-size\">*\u66f4\u65b0\u65e5\uff1a2025.11.28<\/p>\n\n\n\n<p>\u671d\u3001\u7814\u7a76\u5ba4\u306b\u884c\u3063\u3066LB\u30d7\u30ec\u30fc\u30c8\u78ba\u8a8d\u3057\u305f\u3068\u3053\u308d\u3001<\/p>\n\n\n\n<p><span style=\"font-size: 14pt\"><strong>\u30b3\u3001\u30b3\u30ed\u30cb\u30fc\u304c\u751f\u3048\u3066\u306a\u3044<\/strong>\u3002\u3002\u3002<\/span><\/p>\n\n\n\n<p>\u306a\u3093\u3066\u4f53\u9a13\u3092\u3057\u305f\u3053\u3068\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\uff1f<br>\u751f\u3048\u3066\u3044\u308b\u306f\u305a\u306e\u30b3\u30ed\u30cb\u30fc\u304c\u751f\u3048\u306a\u304b\u3063\u305f\u6642\u306f\u3001\u671d\u304b\u3089\u5b9f\u9a13\u306e\u3084\u308b\u6c17\u304c\u305d\u304c\u308c\u3066\u3057\u307e\u3044\u307e\u3059\u3088\u306d\u3002<\/p>\n\n\n\n<p>\u5927\u8178\u83cc\u306e\u5f62\u8cea\u8ee2\u63db\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u306e\u306b\u306f\u3001\u591a\u304f\u306e\u7406\u7531\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u5927\u8178\u83cc\u306e\u5f62\u8cea\u8ee2\u63db\u306b\u3064\u3044\u3066\u3001\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e08\u3064\u3068\u305d\u306e\u5bfe\u7b56\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u306e\u3067\u3001\u5f62\u8cea\u8ee2\u63db\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u304b\u3063\u305f\u969b\u306e\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\uff01<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u305d\u3082\u305d\u3082\u5f62\u8cea\u8ee2\u63db\u3068\u306f\">\u305d\u3082\u305d\u3082\u5f62\u8cea\u8ee2\u63db\u3068\u306f\uff1f<\/h2>\n\n\n\n<p>\u5f62\u8cea\u8ee2\u63db\uff08\u30c8\u30e9\u30f3\u30b9\u30d5\u30a9\u30fc\u30e1\u30fc\u30b7\u30e7\u30f3\uff09\u306f\u3001<strong>\u7d30\u83cc\u7d30\u80de\u306b\u5916\u90e8\u304b\u3089\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u3057\u3066\u83cc\u306e\u907a\u4f1d\u7684\u5f62\u8cea\u3092\u5909\u3048\u308b\u3053\u3068<\/strong>\u3092\u610f\u5473\u3057\u3001\u5225\u540d\u3001\u5f62\u8cea\u5c0e\u5165\u3068\u3082\u547c\u3070\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u3053\u306e\u30d7\u30ed\u30bb\u30b9\u306f\u3001\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u3092\u30b5\u30d6\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\uff08\u3042\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u65ad\u7247\u3092\u3001\u5225\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u7d44\u307f\u8fbc\u3080\u3053\u3068\uff09\u3057\u305f\u5f8c\u3001\u5927\u8178\u83cc\u3092\u5229\u7528\u3057\u3066\u30bf\u30fc\u30b2\u30c3\u30c8DNA\u65ad\u7247\u3092\u542b\u3093\u3060\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5897\u3084\u3059\u305f\u3081\u306b\u884c\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u5c1a\u3001\u5f62\u8cea\u8ee2\u63db\u5f8c\u306f\u3001\u4e3b\u306b\u4e0b\u8a18\u306e\u3088\u3046\u306a\u6d41\u308c\u3067\u5b9f\u9a13\u304c\u884c\u308f\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5897\u5e45\u3057\u305f\u5927\u8178\u83cc\u304b\u3089\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u7cbe\u88fd\u3059\u308b<\/li>\n\n\n\n<li>\u57f9\u990a\u7d30\u80de\u7b49\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\uff08\u52d5\u7269\u7d30\u80de\u306b\u5bfe\u3057\u3066\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\uff09\u3059\u308b<\/li>\n\n\n\n<li>\u76ee\u7684\u306eRNA\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u57f9\u990a\u7d30\u80de\u7b49\u5185\u3067\u7523\u751f\u3055\u305b\u308b<\/li>\n<\/ol>\n\n\n\n<p>\u3057\u305f\u304c\u3063\u3066\u3001<strong>\u5f62\u8cea\u8ee2\u63db\u3067\u3064\u307e\u305a\u3044\u3066\u3057\u307e\u3046\u3068\u3001\u5f8c\u306e\u5b9f\u9a13\u304c\u30b9\u30e0\u30fc\u30ba\u306b\u9032\u307f\u306b\u304f\u304f\u306a\u3063\u3066\u3057\u307e\u3046<\/strong>\u306e\u3067\u3059\u3002<\/p>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<p>\u3067\u306f\u3001\u4f55\u304c\u539f\u56e0\u3067\u5f62\u8cea\u8ee2\u63db\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e1-dna\u4e2d\u306e\u4e0d\u7d14\u7269\">\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e1: DNA\u4e2d\u306e\u4e0d\u7d14\u7269<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cloning\/competent-cells-for-transformation\/chemically-competent.html?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb9_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30b1\u30df\u30ab\u30eb\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb<\/a>\u306e\u5834\u5408\u3001\u30d5\u30a7\u30ce\u30fc\u30eb\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u754c\u9762\u6d3b\u6027\u5264\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u3092DNA\u6eb6\u6db2\u304b\u3089\u53d6\u308a\u9664\u304d\u307e\u3059\u3002\u30a8\u30ec\u30af\u30c8\u30ed\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306e\u5834\u5408\u3001\u5869\u3068\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u8457\u3057\u304f\u963b\u5bb3\u3057\u3001\u30a2\u30fc\u30af\u653e\u96fb\u306e\u30ea\u30b9\u30af\u3092\u5897\u52a0\u3055\u305b\u307e\u3059\u3002\u30d7\u30e9\u30b9\u30df\u30c9DNA\u3092\u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf\u306b\u3088\u308a\u7cbe\u88fd\u3057\u3001\u6ec5\u83cc\u6c34\u307e\u305f\u306f0.5X TE(5 mM Tris-HCl\u30010.5 mM EDTA)\u306b\u6eb6\u89e3\u3059\u308b\u306e\u304c\u304a\u52e7\u3081\u3067\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e2-\u904e\u5270\u306adna\u91cf-\u904e\u5270\u306a\u6db2\u91cf\">\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e2: \u904e\u5270\u306aDNA\u91cf\u3001\u904e\u5270\u306a\u6db2\u91cf<\/h2>\n\n\n\n<p>100 \u00b5l\u306e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cloning\/competent-cells-for-transformation\/chemically-competent.html?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb9_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30b1\u30df\u30ab\u30eb\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb<\/a>\u3042\u305f\u308a\u30011\u301c10 ng\u306eDNA\u3092\u6dfb\u52a0\u3057\u3001\u6dfb\u52a0\u3059\u308bDNA\u306e\u5bb9\u91cf\u306f5 \u00b5l\u3092\u8d85\u3048\u306a\u3044\u3088\u3046\u306b\u3057\u307e\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cloning\/competent-cells-for-transformation\/electrocompetent-cells.html?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb9_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30a8\u30ec\u30af\u30c8\u30ed\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb<\/a>\u3067\u306f\u30011 \u00b5l(1\uff5e50 ng)\u306eDNA\u6eb6\u6db2\u309220\uff5e25 \u00b5l\u306e\u30bb\u30eb\u306b\u6dfb\u52a0\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e3-\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u53cd\u5fdc\u6db2\u306b\u3088\u308b\u5f62\u8cea\u8ee2\u63db\u306e\u963b\u5bb3\">\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e3: \u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u53cd\u5fdc\u6db2\u306b\u3088\u308b\u5f62\u8cea\u8ee2\u63db\u306e\u963b\u5bb3<\/h2>\n\n\n\n<p>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u53cd\u5fdc\u6df7\u5408\u6db2\u3092\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306b\u52a0\u3048\u308b\u524d\u306b\u3001\u53ef\u80fd\u3067\u3042\u308c\u307010 mM Tris-HCl(pH 7.5)\u30011 mM EDTA\u6eb6\u6db2\u3001\u3042\u308b\u3044\u306f\u84b8\u7559\u6c34\u30675\u500d\u7a0b\u5ea6\u306b\u5e0c\u91c8\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e4-\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306e\u4e0d\u9069\u5207\u306a\u53d6\u6271\u3044\">\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e4:\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306e\u4e0d\u9069\u5207\u306a\u53d6\u6271\u3044<\/h2>\n\n\n\n<p>\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306f-80\u00b0C\u306b\u3066\u4fdd\u7ba1\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u3092\u6db2\u4f53\u7a92\u7d20\u306b\u4fdd\u5b58\u3057\u306a\u3044\u3067\u304f\u3060\u3055\u3044\u3002\u51cd\u7d50\u878d\u89e3\u306e\u7e70\u308a\u8fd4\u3057\u306f\u3067\u304d\u308b\u3060\u3051\u907f\u3051\u3001\u672a\u4f7f\u7528\u306e\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306f\u5206\u6ce8\u3057\u3066\u3001\u518d\u51cd\u7d50\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5f62\u8cea\u8ee2\u63db\u6642\u306b\u306f\u6c37\u4e0a\u3067\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u3092\u878d\u89e3\u3057\u3001\u76f4\u3061\u306b\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306f\u3057\u306a\u3044\u3067\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e5-\u5927\u8178\u83cc\u306e\u6210\u9577\u304c\u9045\u3044-\u3082\u3057\u304f\u306f\u6210\u9577\u3057\u306a\u3044\">\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e5: \u5927\u8178\u83cc\u306e\u6210\u9577\u304c\u9045\u3044\u3001\u3082\u3057\u304f\u306f\u6210\u9577\u3057\u306a\u3044<\/h2>\n\n\n\n<p>37\u00b0C\u3067\u306f\u306a\u304f30\u00b0C\u3067\u5927\u8178\u83cc\u3092\u57f9\u990a\u3059\u308b\u5834\u5408\u3001\u56de\u5fa9\u6642\u9593\u3092\u5c11\u306a\u304f\u3068\u308290\u5206\u306b\u5ef6\u9577\u3057\u3001\u5f62\u8cea\u8ee2\u63db\u3057\u305f\u30b3\u30ed\u30cb\u30fc\u306e\u57f9\u990a\u6642\u9593\u3082\u9577\u304f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e6-\u6297\u751f\u7269\u8cea-\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3084\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u306a\u3069-\u306b\u3088\u308b\u9078\u629e\u304c\u4e0d\u9069\u5207\">\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e6: \u6297\u751f\u7269\u8cea\uff08\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3084\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u306a\u3069\uff09\u306b\u3088\u308b\u9078\u629e\u304c\u4e0d\u9069\u5207<\/h2>\n\n\n\n<p><strong>\u6b63\u3057\u3044\u6297\u751f\u7269\u8cea\u3092\u6b63\u3057\u3044\u6fc3\u5ea6\u3067<\/strong>\u4f7f\u7528\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u3067\u63a8\u5968\u3057\u3066\u3044\u308b\u4f7f\u7528\u65b9\u6cd5\u3092\u53c2\u7167\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e7-\u5f62\u8cea\u8ee2\u63db\u52b9\u7387\u3092\u6700\u5927\u5316\u3059\u308b\u305f\u3081\u306e\u57f9\u5730\u9078\u629e\">\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e7: \u5f62\u8cea\u8ee2\u63db\u52b9\u7387\u3092\u6700\u5927\u5316\u3059\u308b\u305f\u3081\u306e\u57f9\u5730\u9078\u629e<\/h2>\n\n\n\n<p>\u3053\u308c\u307e\u3067\u306e\u7814\u7a76\u3067\u306f\u3001\u56de\u5fa9\u57f9\u5730\u3068\u3057\u3066SOC\u57f9\u5730\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u3001LB\u57f9\u5730\u3068\u6bd4\u8f03\u3057\u3066\u5f62\u8cea\u8ee2\u63db\u52b9\u7387\u304c\u5411\u4e0a\u3059\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059\u3002SOC\u57f9\u5730\u306b\u306f\u30b0\u30eb\u30b3\u30fc\u30b9\u3068MgCl\u2082\u304c\u542b\u307e\u308c\u3066\u304a\u308a\u3001\u3053\u308c\u3089\u306f\u6297\u751f\u7269\u8cea\u5165\u308a\u306e\u9078\u629e\u57f9\u5730\u3067\u57f9\u990a\u3059\u308b\u524d\u306e\u30d7\u30ec\u57f9\u990a\u306e\u6bb5\u968e\u3067\u7d30\u80de\u306e\u56de\u5fa9\u3092\u4fc3\u9032\u3057\u307e\u3059\u3002\u7279\u306b\u96e3\u3057\u3044\u5f62\u8cea\u8ee2\u63db\u3084\u5927\u304d\u306a\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f\u3001SOC\u57f9\u5730\u306e\u4f7f\u7528\u3092\u691c\u8a0e\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e8-\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306e\u54c1\u8cea\u3068\u9069\u5207\u306a\u83cc\u682a\u9078\u629e\">\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u305d\u306e8: \u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306e\u54c1\u8cea\u3068\u9069\u5207\u306a\u83cc\u682a\u9078\u629e<\/h2>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p>\u5f62\u8cea\u8ee2\u63db\u52b9\u7387\u3092\u6700\u5927\u5316\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306e\u54c1\u8cea\u304c\u6975\u3081\u3066\u91cd\u8981\u3067\u3059\u3002\u5bfe\u6570\u5897\u6b96\u671f\u4e2d\u671f\uff08OD600\u304c0.4\uff5e0.9\u306e\u9593\uff09\u3067\u7d30\u80de\u3092\u63a1\u53d6\u3059\u308b\u3053\u3068\u304c\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u3092\u4f5c\u6210\u3059\u308b\u969b\u306e\u6761\u4ef6\u3068\u3057\u3066\u91cd\u8981\u3067\u3042\u308a\u3001\u7279\u306b\u9ad8\u3044\u5f62\u8cea\u8ee2\u63db\u52b9\u7387\uff0810<sup>8<\/sup> CFU<sup>\u203b<\/sup>\/\u00b5g\u4ee5\u4e0a\uff09\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\u3001\u3053\u306e\u6761\u4ef6\u3092\u6e80\u305f\u3057\u305f\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\u307e\u305f\u3001\u7528\u9014\u306b\u5408\u308f\u305b\u305f\u9069\u5207\u306a\u83cc\u682a\u306e\u9078\u629e\u3082\u91cd\u8981\u3067\u3059\u3002\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5897\u3084\u3057\u3066\u62bd\u51fa\u3059\u308b\u306e\u306b\u9069\u3057\u305f\u83cc\u682a\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5927\u91cf\u767a\u73fe\u3059\u308b\u306e\u306b\u9069\u3057\u305f\u83cc\u682a\u306a\u3069\u3001\u76ee\u7684\u306b\u5fdc\u3058\u3066\u4f7f\u3044\u5206\u3051\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u203bCFU = colony forming unit<\/p>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u3053\u308c\u3060\u3051\u306f\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u57fa\u790e\u77e5\u8b58-\u30b3\u30b3\u306b\u3042\u308a\u307e\u3059\">\u3053\u308c\u3060\u3051\u306f\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u57fa\u790e\u77e5\u8b58\u3001\u30b3\u30b3\u306b\u3042\u308a\u307e\u3059\uff01<\/h2>\n\n\n\n<p>Invitrogen \u5206\u5b50\u751f\u7269\u5b66\u6559\u5ba4\u3067\u306f\u3001\u5206\u5b50\u751f\u7269\u5b66\u306e\u6559\u80b2\u306e\u30e1\u30a4\u30f3\u3068\u3057\u3066\u3001\u521d\u5fc3\u8005\u3060\u3051\u3067\u306a\u304f\u7d4c\u9a13\u306e\u3042\u308b\u5206\u5b50\u751f\u7269\u5b66\u8005\u306e\u305f\u3081\u306b\u3001\u8c4a\u5bcc\u3067\u4fe1\u983c\u3067\u304d\u308b\u6280\u8853\u7684\u306a\u5185\u5bb9\u3092\u3054\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u57fa\u790e\u77e5\u8b58\u3092\u5b66\u3076\u6642\u306b\u306f\u3044\u3064\u3067\u3082\u3001Invitrogen \u5206\u5b50\u751f\u7269\u5b66\u6559\u5ba4\u306e\u7121\u6599PDF\u3092\u6d3b\u7528\u3057\u3066\u304f\u3060\u3055\u3044\uff01<\/p>\n\n\n\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/mol-bio-school.html?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb9_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5927\u8178\u83cc\u306e\u5f62\u8cea\u8ee2\u63db\u304c\u4e88\u671f\u3057\u3066\u3044\u305f\u307b\u3069\u52b9\u7387\u7684\u3067\u306a\u3044\u3053\u3068\u306b\u306f\u3001\u591a\u304f\u306e\u7406\u7531\u304c\u3042\u308a\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u5927\u8178\u83cc\u306e\u5f62\u8cea\u8ee2\u63db\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u539f\u56e0\u3068\u305d\u306e\u5bfe\u7b56\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u306e\u3067\u3001\u5f62\u8cea\u8ee2\u63db\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u304b\u3063\u305f\u969b\u306e\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\uff01<\/p>\n","protected":false},"author":975,"featured_media":2770,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[14,62],"division":[],"class_list":{"0":"post-479","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-cloning","9":"tag-transformation","10":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- 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