{"id":487,"date":"2016-09-27T00:00:00","date_gmt":"2016-09-27T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/optimizing-western-blot-transfer\/"},"modified":"2025-01-22T14:51:20","modified_gmt":"2025-01-22T05:51:20","slug":"optimizing-western-blot-transfer","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/optimizing-western-blot-transfer\/","title":{"rendered":"\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u304a\u3051\u308b\u8ee2\u5199\u52b9\u7387\u306e\u6700\u9069\u5316"},"content":{"rendered":"

\u306f\u3058\u3081\u306b<\/h2>\n

SDS\u30b1\u3099\u30eb\u304b\u3089\u306e\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306e\u96fb\u6c17\u30d5\u3099\u30ed\u30c3\u30c6\u30a3\u30f3\u30af\u3099\u3092\u6700\u9069\u5316\u3059\u308b\u305f\u3081\u306b\u306f\u6570\u591a\u304f\u306e\u30cf\u309a\u30e9\u30e1\u30fc\u30bf\u3092\u8003\u616e\u3059\u308b\u5fc5\u8981\u304b\u3099\u3042\u308a\u307e\u3059\u3002\u76ee\u6a19\u306f\u30b1\u3099\u30eb\u304b\u3089\u3059\u3078\u3099\u3066\u306e\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u3092\u8ee2\u5199\u3055\u305b\u3001\u8ee2\u5199\u819c\u306b\u5b9a\u91cf\u7684\u306b\u8ee2\u5199\u3055\u305b\u308b\u3053\u3068\u3066\u3099\u3059\u3002\u9ad8\u3044\u8ee2\u5199\u52b9\u7387\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\u3001\u3068\u304f\u306b\u5927\u304d\u306a\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u3068\u5c0f\u3055\u306a\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306e\u8ee2\u5199\u3092\u6700\u9069\u5316\u3059\u308b\u5fc5\u8981\u304b\u3099\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u8ee2\u5199\u5f8c\u3001\u30b1\u3099\u30eb\u306b\u306f\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306f\u6b8b\u5b58\u3057\u3066\u3044\u306a\u3044\u306f\u3059\u3099\u3066\u3099\u3059\u3002\u3053\u308c\u306f\u8ee2\u5199\u5f8c\u306b\u30b1\u3099\u30eb\u3092\u67d3\u8272\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u30e2\u30cb\u30bf\u30fc\u3059\u308b\u3053\u3068\u304b\u3099\u3066\u3099\u304d\u307e\u3059\u3002<\/strong>\u30b1\u3099\u30eb\u304b\u3089\u8ee2\u5199\u3057\u305f\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306f\u30b1\u3099\u30eb\u3068\u63a5\u89e6\u3057\u3066\u3044\u305f\u819c\u306e\u4e0a\u306b\u898b\u3048\u308b\u306f\u3059\u3099\u3066\u3099\u3059\u3002\u7591\u308f\u3057\u3044\u5834\u5408\u306f\u30d5\u3099\u30ed\u30c3\u30c8\u306b\u4e8c\u756a\u76ee\u306e\u300c\u30cf\u3099\u30c3\u30af\u30a2\u30c3\u30d5\u309a\u300d\u819c\u3092\u7528\u3044\u3001\u5f8c\u306b\u3053\u308c\u3092\u67d3\u8272\u3057\u3066\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u304b\u30991\u679a\u76ee\u306e\u819c\u3092\u901a\u904e\u3057\u305f\u304b\u3068\u3099\u3046\u304b\u3092\u30c1\u30a7\u30c3\u30af\u3059\u308b\u3053\u3068\u304b\u3099\u3066\u3099\u304d\u307e\u3059\u3002<\/strong>\u30b1\u3099\u30eb\u306b\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u304b\u3099\u6b8b\u308a\u3001\u819c\u3068\u306e\u7d50\u5408\u304b\u3099\u5f31\u3044\u5834\u5408\u306f\u4ee5\u4e0b\u306e\u30cf\u309a\u30e9\u30e1\u30fc\u30bf\u3092\u8abf\u6574\u3057\u307e\u3059\u3002<\/p>\n

SDS\u3068\u30a2\u30eb\u30b3\u30fc\u30eb\u306e\u4f5c\u7528<\/h2>\n

\u305f\u3044\u3066\u3044\u306e\u30d5\u3099\u30ed\u30c3\u30c6\u30a3\u30f3\u30af\u3099\u30d5\u309a\u30ed\u30c8\u30b3\u30fc\u30eb\u3066\u3099\u306fSDS\u3068\u30a2\u30eb\u30b3\u30fc\u30eb\u304b\u3099\u7528\u3044\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u3053\u308c\u3089\u306f\u8ee2\u5199\u306e\u6210\u5426\u306b\u95a2\u3057\u3066\u306f\u9006\u306e\u4f5c\u7528\u3092\u6709\u3057\u3066\u304a\u308a\u3001\u8ee2\u5199\u3055\u305b\u308b\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306e\u6027\u8cea\u306b\u5fdc\u3057\u3099\u3066\u6700\u9069\u5316\u3059\u308b\u5fc5\u8981\u304b\u3099\u3042\u308a\u307e\u3059\u3002<\/p>\n

SDS\u306f\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u304b\u3099\u79fb\u52d5\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u3066\u3099\u3042\u308a\u3001\u30b1\u3099\u30eb\u304b\u3089\u306e\u5927\u304d\u306a\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306e\u8ee2\u5199\u3092\u4fc3\u9032\u3059\u308b\u4e0a\u3066\u3099\u3068\u304f\u306b\u6709\u7528\u3066\u3099\u3059\u3002<\/strong>\u3057\u304b\u3057\u3001\u819c\u3078\u306e\u7d50\u5408\u306f\u758e\u6c34\u6027\u306e\u76f8\u4e92\u4f5c\u7528\u3092\u5fc5\u8981\u3068\u3059\u308b\u306e\u3066\u3099\u3001SDS\u304b\u3099\u591a\u3059\u304d\u3099\u308b\u3068\u3068\u304f\u306b\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u3078\u306e\u7d50\u5408\u304b\u3099\u59a8\u3051\u3099\u3089\u308c\u308b\u3053\u3068\u304b\u3099\u3042\u308a\u307e\u3059\u3002\u9006\u306b\u3042\u307e\u308a\u306b\u591a\u304f\u306eSDS\u304b\u3099\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u304b\u3089\u596a\u308f\u308c\u308b\u3068\u30b1\u3099\u30eb\u304b\u3089\u8ee2\u5199\u3057\u306a\u3044\u3053\u3068\u304b\u3099\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u4e00\u822c\u539f\u5247\u3068\u3057\u3066\u3001\u819c\u3078\u306e\u7d50\u5408\u52b9\u7387\u306f\u3088\u3044\u304b\u3099\u4e00\u90e8\u306e\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u304b\u3099\u30b1\u3099\u30eb\u306b\u6b8b\u3063\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\u30cf\u3099\u30c3\u30d5\u30a1\u30fc\u306b0.01%\u306eSDS\u3092\u6dfb\u52a0\u3059\u308b\u3068\u8ee2\u5199\u304b\u3099\u4fc3\u9032\u3055\u308c\u308b\u3053\u3068\u304b\u3099\u3042\u308a\u307e\u3059\u3002<\/strong>\u63a8\u5968\u30d5\u309a\u30ed\u30c8\u30b3\u30fc\u30eb\u3066\u3099\u306f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\u30cf\u3099\u30c3\u30d5\u30a1\u30fc\u306bSDS\u306f\u542b\u307e\u308c\u3066\u3044\u307e\u305b\u3093\u304b\u3099\u3001\u96fb\u6c17\u6cf3\u52d5\u5f8c\u306b\u30b1\u3099\u30eb\u3092\u6d78\u3055\u306a\u3044\u3053\u3068\u304b\u3099\u5fc5\u8981\u3066\u3099\u3059\u3002\u3064\u307e\u308a\u8ee2\u5199\u304b\u3099\u52b9\u7387\u7684\u306b\u884c\u306a\u308f\u308c\u308b\u305f\u3081\u306b\u306f\u30b1\u3099\u30eb\u306b\u6b8b\u5b58\u3057\u3066\u3044\u308bSDS\u3066\u3099\u5341\u5206<\/strong>\u306a\u306e\u3066\u3099\u3059\u3002<\/p>\n

\u4ed6\u65b9\u3001\u30a2\u30eb\u30b3\u30fc\u30eb\u306f\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u304b\u3089\u4e00\u90e8\u306eSDS\u3092\u596a\u3046\u3053\u3068\u306b\u3088\u3063\u3066\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306e\u819c\u3078\u306e\u758e\u6c34\u6027\u7d50\u5408\u3092\u4fc3\u9032\u3059\u308b\u305f\u3081\u306b\u7528\u3044\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u901a\u5e38\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\u30cf\u3099\u30c3\u30d5\u30a1\u30fc\u306b20%\u306e\u30e1\u30bf\u30ce\u30fc\u30eb\u3092\u542b\u307e\u305b\u308b\u3053\u3068\u306b\u3088\u308a\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\u3078\u306e\u7d50\u5408\u304b\u3099\u5411\u4e0a\u3057\u307e\u3059\u3002<\/strong><\/p>\n

\u30b1\u3099\u30eb\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c8\u3099\u306e\u6fc3\u5ea6<\/h2>\n

\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c8\u3099\u306e\u6fc3\u5ea6\u304b\u3099\u4f4e\u3044\u307b\u3068\u3099\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306f\u8ee2\u5199\u3057\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002<\/strong>\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u3092\u5206\u96e2\u3059\u308b\u4e0a\u3066\u3099\u3066\u3099\u304d\u308b\u305f\u3099\u3051\u4f4e\u3044\u6fc3\u5ea6\u3092\u9078\u629e\u3057\u307e\u3059\u3002\u30af\u3099\u30e9\u30b7\u3099\u30a8\u30f3\u30c8\u30b1\u3099\u30eb\u306f\u30b1\u3099\u30eb\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u306e\u591a\u5b54\u6027\u304b\u3099\u3055\u307e\u3055\u3099\u307e\u306a\u5927\u304d\u3055\u306e\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306b\u3088\u304f\u9069\u5408\u3057\u3066\u3044\u308b\u306e\u3066\u3099\u3001\u5e83\u7bc4\u306a\u5927\u304d\u3055\u306e\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u3092\u30d5\u3099\u30ed\u30c3\u30c8\u3059\u308b\u4e0a\u3066\u3099\u7406\u60f3\u7684\u3066\u3099\u3059\u3002<\/p>\n

\u30b1\u3099\u30eb\u306e\u539a\u3055<\/h2>\n

\u4ed6\u306e\u3059\u3078\u3099\u3066\u306e\u6761\u4ef6\u304b\u3099\u540c\u3057\u3099\u3066\u3099\u3042\u308c\u306f\u3099\u3001\u30b1\u3099\u30eb\u304b\u3099\u8584\u3044\u307b\u3068\u3099\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306f\u5bb9\u6613\u306b\u8ee2\u5199\u3055\u308c\u307e\u3059\u3002<\/strong>\u3057\u305f\u304b\u3099\u3063\u3066\u3001\u30b5\u30f3\u30d5\u309a\u30eb\u5bb9\u91cf\u304b\u3099\u8a31\u3059\u9650\u308a\u539a\u30551.5 mm\u3088\u308a1.0 mm\u306e\u30b1\u3099\u30eb\u3092\u9078\u629e\u3057\u307e\u3059\u3002<\/p>\n

\u96fb\u5834(\u96fb\u5727\u00d7\u6642\u9593)<\/h2>\n

\u96fb\u5727\u304b\u3099\u4f4e\u3059\u304d\u3099\u305f\u308a\u8ee2\u5199\u6642\u9593\u304b\u3099\u77ed\u3059\u304d\u3099\u308b\u3068\u4e00\u90e8\u306e\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u304b\u3099\u30b1\u3099\u30eb\u306b\u6b8b\u308a\u307e\u3059\u3002\u96fb\u5727\u304b\u3099\u9ad8\u3059\u304d\u3099\u308b\u3068\u5c0f\u3055\u306a\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306f\u7d50\u5408\u3059\u308b\u524d\u306b\u819c\u3092\u901a\u904e\u3057\u3066\u3057\u307e\u3044\u307e\u3059\u3002<\/strong>\u63a8\u5968\u6761\u4ef6\u3066\u3099\u30d5\u3099\u30ed\u30c3\u30c6\u30a3\u30f3\u30af\u3099\u3057\u305f\u5f8c\u306b\u30b1\u3099\u30eb\u306b\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u304b\u3099\u6b8b\u308b\u5834\u5408\u306f\u30015\u30db\u3099\u30eb\u30c8\u3092\u8d85\u3048\u306a\u3044\u7a0b\u5ea6\u306e\u96fb\u5727\u3092\u4e0a\u3051\u3099\u308b\u306e\u304b\u3099\u6709\u52b9\u3066\u3099\u3059\u3002\u3057\u304b\u3057\u3001\u3044\u3063\u305f\u3093SDS\u304b\u3099\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u304b\u3089\u5916\u308c\u308b\u3068\u8ee2\u5199\u6642\u9593\u3092\u9577\u304f\u3057\u3066\u3082\u96fb\u5727\u3092\u4e0a\u3051\u3099\u3066\u3082\u52b9\u679c\u304b\u3099\u306a\u3044\u3053\u3068\u306b\u6ce8\u610f\u3057\u3066\u4e0b\u3055\u3044\u3002\u305f\u3044\u3066\u3044\u306e\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306f\u3044\u3063\u305f\u3093\u7d50\u5408\u3059\u308b\u3068\u8ee2\u5199\u6642\u9593\u3092\u4f38\u306f\u3099\u3057\u3066\u3082\u819c\u4e0a\u306b\u7559\u307e\u308a\u307e\u3059\u3002<\/p>\n

\u819c\u306e\u7a2e\u985e<\/h2>\n

\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\u3078\u306e\u7d50\u5408\u306f\u4e3b\u306b\u758e\u6c34\u6027\u7d50\u5408\u306b\u3088\u308b\u3082\u306e\u3066\u3099\u3059\u3002\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u306f\u591a\u76ee\u7684\u306e\u3059\u304f\u3099\u308c\u305f\u819c\u3066\u3099\u3059\u3002\u5c0f\u3055\u306a\u30d8\u309a\u30d5\u309a\u30c1\u30c8\u3099\u3066\u3099\u306f\u5b54\u306e\u5c0f\u3055\u306a\u819c\u3092\u304a\u85a6\u3081\u3057\u307e\u3059\u3002<\/strong><\/p>\n

PVDF\u306f\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u3088\u308a\u758e\u6c34\u6027\u304b\u3099\u9ad8\u304f\u3001\u3088\u308a\u5f37\u529b\u306b\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u3068\u7d50\u5408\u3057\u3001\u30d5\u3099\u30ed\u30c3\u30c6\u30a3\u30f3\u30af\u3099\u7cfb\u3066\u3099\u306f\u3088\u308a\u591a\u304f\u306eSDS\u306b\u8010\u3048\u307e\u3059\u3002\u3057\u305f\u304b\u3099\u3063\u3066\u3001\u4e00\u822c\u306bPVDF\u306f\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u3088\u308a\u53b3\u683c\u306a\u30d5\u3099\u30ed\u30c3\u30ad\u30f3\u30af\u3099\u6761\u4ef6\u3092\u5fc5\u8981\u3068\u3057\u307e\u3059\u3002<\/strong>PVDF\u306f\u30bf\u30f3\u30cf\u309a\u30af\u8cea\u306e\u914d\u5217\u6c7a\u5b9a\u306b\u9069\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

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\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

 <\/p>\n

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