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\u7d99\u4ee3\u3068\u306f?<\/h2>\n

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\"guidelines-for-maintaining-cultured-cells-fig\"<\/a>

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\u7d99\u4ee3\u306e\u6642\u671f<\/h2>\n

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\u7d30\u80de\u5bc6\u5ea6<\/h3>\n

\u54fa\u4e73\u985e\u7d30\u80de<\/h4>\n

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\u6606\u866b\u7d30\u80de<\/h4>\n

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\u57f9\u5730\u306e\u6804\u990a\u7269\u306e\u67af\u6e07<\/h3>\n

\u54fa\u4e73\u985e\u7d30\u80de<\/h4>\n

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\u6606\u866b\u7d30\u80de<\/h4>\n

\u6606\u866b\u7d30\u80de\u306f\u4e00\u822c\u306b\u3001\u54fa\u4e73\u985e\u7d30\u80de\u306b\u4f7f\u7528\u3055\u308c\u308b\u57f9\u5730\u306b\u6bd4\u3079\u3066\u3088\u308a\u9178\u6027\u306e\u5897\u6b96\u57f9\u5730\u3067\u57f9\u990a\u3055\u308c\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001Sf9\u7d30\u80de\u306e\u57f9\u990a\u306b\u4f7f\u7528\u3055\u308c\u308bTNM-FH\u304a\u3088\u3073\u30b0\u30ec\u30fc\u30b9\u57f9\u5730\u306epH\u306f6.2\u3067\u3059\u3002\u54fa\u4e73\u985e\u7d30\u80de\u3068\u306f\u7570\u306a\u308a\u3001\u6606\u866b\u7d30\u80de\u306b\u304a\u3044\u3066\u306f\u3001\u6210\u9577\u306b\u4f34\u3044pH\u306f\u5f90\u3005\u306b\u4e0a\u6607\u3057\u307e\u3059\u304c\u3001\u4e00\u822c\u306bpH6.4\u3092\u8d85\u3048\u308b\u3053\u3068\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u54fa\u4e73\u985e\u7d30\u80de\u3068\u540c\u69d8\u306b\u3001\u6606\u866b\u7d30\u80de\u3082\u5bc6\u5ea6\u304c\u4e0a\u6607\u3059\u308b\u3068\u5897\u6b96\u57f9\u5730\u306epH\u306f\u4f4e\u4e0b\u3057\u59cb\u3081\u307e\u3059\u3002<\/p>\n

\u7d99\u4ee3\u306e\u30b9\u30b1\u30b8\u30e5\u30fc\u30eb<\/h3>\n

\u53b3\u5bc6\u306a\u30b9\u30b1\u30b8\u30e5\u30fc\u30eb\u306b\u5f93\u3063\u3066\u7d30\u80de\u306e\u690d\u3048\u7d99\u304e\u3092\u884c\u3046\u3053\u3068\u3067\u3001\u7d30\u80de\u306e\u6027\u8cea\u306e\u518d\u73fe\u6027\u304c\u4fdd\u8a3c\u3055\u308c\u3001\u305d\u306e\u5065\u5eb7\u72b6\u614b\u3092\u76e3\u8996\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u3054\u4f7f\u7528\u306b\u306a\u308b\u7d30\u80de\u306e\u64ad\u7a2e\u5bc6\u5ea6\u3092\u3001\u5f53\u8a72\u7d30\u80de\u306b\u9069\u5207\u306a\u6210\u9577\u901f\u5ea6\u304a\u3088\u3073\u53ce\u7387\u304c\u5f97\u3089\u308c\u308b\u307e\u3067\u3001\u4e00\u5b9a\u306e\u5024\u304b\u3089\u5909\u5316\u3055\u305b\u3066\u304f\u3060\u3055\u3044\u3002\u6210\u9577\u30d1\u30bf\u30fc\u30f3\u304b\u3089\u306e\u9038\u8131\u306f\u3001\u57f9\u990a\u7d30\u80de\u304c\u4e0d\u5065\u5eb7\u3067\u3042\u308b\uff08\u4f8b\uff1a \u52a3\u5316\u304a\u3088\u3073\u6c5a\u67d3\uff09\u3001\u307e\u305f\u306f\u7d30\u80de\u57f9\u990a\u7cfb\u306e\u6210\u5206\u304c\u6b63\u3057\u304f\u6a5f\u80fd\u3057\u3066\u3044\u306a\u3044\uff08\u4f8b\uff1a\u6e29\u5ea6\u304c\u6700\u9069\u3067\u306a\u3044\u3001\u307e\u305f\u306f\u57f9\u5730\u304c\u53e4\u3059\u304e\u308b\uff09\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002\u8a73\u7d30\u306a\u7d30\u80de\u57f9\u990a\u8a18\u9332\u3092\u4f5c\u6210\u3057\u3001\u88dc\u7d66\u304a\u3088\u3073\u7d99\u4ee3\u306e\u30b9\u30b1\u30b8\u30e5\u30fc\u30eb\u3001\u4f7f\u7528\u3057\u305f\u57f9\u5730\u306e\u7a2e\u985e\u3001\u5206\u96e2\u6cd5\u3001\u5206\u96e2\u6bd4\u3001\u5f62\u614b\u5b66\u7684\u89b3\u5bdf\u7d50\u679c\u3001\u64ad\u7a2e\u6fc3\u5ea6\u3001\u53ce\u7387\u304a\u3088\u3073\u6297\u751f\u7269\u8cea\u306e\u4f7f\u7528\u3092\u8a18\u9332\u3055\u308c\u308b\u3053\u3068\u3092\u5f37\u304f\u63a8\u5968\u3057\u307e\u3059\u3002<\/p>\n

\u5b9f\u9a13\u304a\u3088\u3073\u7279\u5225\u306a\u64cd\u4f5c\uff08\u4f8b\uff1a \u57f9\u5730\u306e\u7a2e\u985e\u306e\u5909\u66f4\uff09\u306f\u3001\u7d99\u4ee3\u306e\u30b9\u30b1\u30b8\u30e5\u30fc\u30eb\u306b\u5408\u308f\u305b\u3066\u884c\u3046\u306e\u304c\u6700\u3082\u7406\u60f3\u7684\u3067\u3059\u3002\u5b9f\u9a13\u8a08\u753b\u304c\u3001\u78ba\u7acb\u3055\u308c\u305f\u7d99\u4ee3\u306e\u30b9\u30b1\u30b8\u30e5\u30fc\u30eb\u306b\u5408\u81f4\u3057\u306a\u3044\u5834\u5408\u3001\u7d30\u80de\u304c\u8a98\u5c0e\u671f\u306b\u3042\u308b\u9593\u3001\u307e\u305f\u306f\u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\u306b\u9054\u3057\u3066\u5897\u6b96\u304c\u505c\u6b62\u3057\u3066\u3044\u308b\u6642\u306b\u306f\u3001\u6c7a\u3057\u3066\u690d\u3048\u7d99\u304e\u3092\u884c\u308f\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u4e00\u822c\u7684\u306a\u7d30\u80de\u7cfb\u306b\u63a8\u5968\u3055\u308c\u308b\u57f9\u5730<\/h2>\n

\u4e0d\u6b7b\u5316\u54fa\u4e73\u985e\u7d30\u80de\u7cfb\u306f\u3001\u8840\u6e05\u3092\u6dfb\u52a0\u3057\u305fMEM<\/a>\u306a\u3069\u306e\u6bd4\u8f03\u7684\u5358\u7d14\u306a\u57f9\u5730\u4e0a\u3067\u7dad\u6301\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3001\u307e\u305fMEM\u4e2d\u3067\u57f9\u990a\u3055\u308c\u305f\u7d30\u80de\u306f\u3001\u304a\u305d\u3089\u304fDMEM<\/a>\u304a\u3088\u3073Medium 199\u57f9\u5730<\/a>\u306b\u304a\u3044\u3066\u3082\u3001\u540c\u69d8\u306b\u5bb9\u6613\u306b\u57f9\u990a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u7279\u5225\u306a\u6a5f\u80fd\u304c\u767a\u73fe\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u306b\u306f\u3001\u3088\u308a\u8907\u96d1\u306a\u57f9\u5730\u304c\u5fc5\u8981\u306b\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u7279\u5b9a\u306e\u7d30\u80de\u306b\u5bfe\u3057\u3066\u9069\u5207\u306a\u57f9\u5730\u3092\u9078\u629e\u3059\u308b\u305f\u3081\u306e\u60c5\u5831\u306f\u3001\u4e00\u822c\u306b\u6587\u732e\u304b\u3089\u5165\u624b\u53ef\u80fd\u3067\u3042\u308a\u3001\u7d30\u80de\u306e\u4f9b\u7d66\u8005\u307e\u305f\u306f\u7d30\u80de\u30d0\u30f3\u30af\u304b\u3089\u3082\u5165\u624b\u53ef\u80fd\u306a\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u3054\u4f7f\u7528\u306e\u7d30\u80de\u578b\u306b\u95a2\u3059\u308b\u60c5\u5831\u304c\u5165\u624b\u4e0d\u53ef\u80fd\u306a\u5834\u5408\u3001\u5897\u6b96\u57f9\u5730\u304a\u3088\u3073\u8840\u6e05\u3092\u7d4c\u9a13\u306b\u57fa\u3065\u3044\u3066\u9078\u629e\u3059\u308b\u304b\u3001\u307e\u305f\u306f\u6570\u7a2e\u985e\u306e\u7570\u306a\u308b\u57f9\u5730\u3092\u8a66\u9a13\u3057\u3066\u3001\u6700\u9069\u306a\u7d50\u679c\u306e\u5f97\u3089\u308c\u308b\u57f9\u5730\u3092\u898b\u3064\u3051\u51fa\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4e00\u822c\u7684\u306b\u306f\u3001\u63a5\u7740\u7d30\u80de\u306b\u306fMEM<\/a>\u3001\u6d6e\u904a\u7d30\u80de\u306b\u306fRPMI-1640<\/a>\u304c\u3001\u57f9\u5730\u691c\u8a0e\u306e\u826f\u597d\u306a\u51fa\u767a\u70b9\u3068\u3057\u3066\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u4e0b\u8868\u306e\u6761\u4ef6\u306f\u3001\u65b0\u3057\u3044\u54fa\u4e73\u985e\u7d30\u80de\u57f9\u990a\u3092\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u3059\u308b\u969b\u306e\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u3068\u3057\u3066\u3054\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/p>\n

\u6606\u866b\u7d30\u80de\u306f\u3001\u54fa\u4e73\u985e\u7d30\u80de\u306b\u4f7f\u7528\u3055\u308c\u308b\u5897\u6b96\u57f9\u5730\u3088\u308a\u3082\u4e00\u822c\u306b\u3088\u308a\u9178\u6027\u5ea6\u306e\u9ad8\u3044\u5897\u6b96\u57f9\u5730\u3067\u3042\u308b\u3001TNM-FH\u304a\u3088\u3073\u30b0\u30ec\u30fc\u30b9\u57f9\u5730\u306a\u3069\u7528\u3044\u3066\u57f9\u990a\u3055\u308c\u307e\u3059\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u88681 \u54fa\u4e73\u985e\u57f9\u990a\u7d30\u80de\u306b\u9069\u3057\u305f\u57f9\u5730\u307e\u3068\u3081<\/caption>\n
\u54fa\u4e73\u985e\u57f9\u990a\u7d30\u80de<\/strong><\/th>\n<\/tr>\n
\u7d30\u80de\u7cfb<\/strong><\/th>\n\u7d30\u80de\u578b<\/strong><\/th>\n\u7a2e<\/strong><\/th>\n\u7d44\u7e54<\/strong><\/th>\n\u57f9\u5730\u203b<\/sup><\/strong><\/th>\n<\/tr>\n
293<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u80da\u814e\u81d3<\/td>\nMEM\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
3T6<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30de\u30a6\u30b9<\/td>\n\u80da<\/td>\nDMEM\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
A549<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u80ba\u764c<\/td>\nF-12K\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
A9<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30de\u30a6\u30b9<\/td>\n\u7d50\u5408\u7d44\u7e54<\/td>\nDMEM\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
AtT-20<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30de\u30a6\u30b9<\/td>\n\u4e0b\u5782\u4f53\u816b\u760d<\/td>\nF-10, 15% \u30a6\u30de\u8840\u6e05\u304a\u3088\u30732.5% FBS<\/td>\n<\/tr>\n
BALB\/3T3<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30de\u30a6\u30b9<\/td>\n\u80da<\/td>\nDMEM\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
BHK-21<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30cf\u30e0\u30b9\u30bf\u30fc<\/td>\n\u814e\u81d3<\/td>\nGMEM\u304a\u3088\u307310% FBS\u307e\u305f\u306fMEM\u300110% FBS\u304a\u3088\u3073NEAA<\/td>\n<\/tr>\n
BHL-100<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u4e73\u623f<\/td>\nMcCoy’s 5A\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
BT<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30a6\u30b7<\/td>\n\u9f3b\u7532\u4ecb\u7d30\u80de<\/td>\nMEM\u300110% FBS\u304a\u3088\u3073NEAA<\/td>\n<\/tr>\n
Caco-2<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u5927\u8178\u817a\u764c<\/td>\nMEM\u300120% FBS\u304a\u3088\u3073NEAA<\/td>\n<\/tr>\n
Chang<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u809d\u81d3<\/td>\nBME\u304a\u3088\u307310% \u4ed4\u30a6\u30b7\u8840\u6e05<\/td>\n<\/tr>\n
CHO-K1<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30cf\u30e0\u30b9\u30bf\u30fc<\/td>\n\u5375\u5de3<\/td>\nF-12\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
Clone 9<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30e9\u30c3\u30c8<\/td>\n\u809d\u81d3<\/td>\nF-12K\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
Clone M-3<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30de\u30a6\u30b9<\/td>\n\u30e1\u30e9\u30ce\u30fc\u30de<\/td>\nF-10, 15% \u30a6\u30de\u8840\u6e05\u304a\u3088\u30732.5% FBS<\/td>\n<\/tr>\n
COS-1, COS-3, COS-7<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30b5\u30eb<\/td>\n\u814e\u81d3<\/td>\nDMEM\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
CRFK<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30cd\u30b3<\/td>\n\u814e\u81d3<\/td>\nMEM\u300110% FBS\u304a\u3088\u3073NEAA<\/td>\n<\/tr>\n
CV-1<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30b5\u30eb<\/td>\n\u814e\u81d3<\/td>\nMEM\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
D-17<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30a4\u30cc<\/td>\n\u9aa8\u8089\u816b<\/td>\nMEM\u300110% FBS\u304a\u3088\u3073NEAA<\/td>\n<\/tr>\n
Daudi<\/td>\n\u30ea\u30f3\u30d1\u82bd\u7403\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u30ea\u30f3\u30d1\u816b\u60a3\u8005\u306e\u8840\u6db2<\/td>\nRPMI-1640\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
GH1, GH3<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30e9\u30c3\u30c8<\/td>\n\u4e0b\u5782\u4f53\u816b\u760d<\/td>\nF-10, 15% \u30a6\u30de\u8840\u6e05\u304a\u3088\u30732.5% FBS<\/td>\n<\/tr>\n
H9<\/td>\n\u30ea\u30f3\u30d1\u82bd\u7403\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\nT\u7d30\u80de\u30ea\u30f3\u30d1\u816b<\/td>\nRPMI-1640\u304a\u3088\u307320% FBS<\/td>\n<\/tr>\n
HaK<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30cf\u30e0\u30b9\u30bf\u30fc<\/td>\n\u814e\u81d3<\/td>\nBME\u304a\u3088\u307310% \u4ed4\u30a6\u30b7\u8840\u6e05<\/td>\n<\/tr>\n
HCT-15<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u7d50\u8178\u76f4\u8178\u817a\u764c<\/td>\nRPMI-1640\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
HeLa<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u5b50\u5bae\u9838\u764c<\/td>\nMEM\u300110% FBS\u304a\u3088\u3073NEAA (S-MEM\u61f8\u6fc1)<\/td>\n<\/tr>\n
HEp-2<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u5589\u982d\u764c<\/td>\nMEM\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
HL-60<\/td>\n\u30ea\u30f3\u30d1\u82bd\u7403\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u524d\u9aa8\u9ac4\u7403\u6027\u767d\u8840\u75c5<\/td>\nRPMI-1640\u304a\u3088\u307320% FBS<\/td>\n<\/tr>\n
HT-1080<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u7dda\u7dad\u8089\u816b<\/td>\nMEM\u304a\u3088\u307310% HI FBS\u304a\u3088\u3073NEAA<\/td>\n<\/tr>\n
HT-29<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u5927\u8178\u817a\u764c<\/td>\nMcCoy’s 5A\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
HUVEC<\/td>\n\u5185\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u81cd\u5e2f<\/td>\nF-12K\u304a\u3088\u307310% FBS\u304a\u3088\u3073100 \u00b5g\/mL \u30d8\u30d1\u30ea\u30f3<\/td>\n<\/tr>\n
I-10<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30de\u30a6\u30b9<\/td>\n\u7cbe\u5de3\u816b\u760d<\/td>\nF-10, 15% \u30a6\u30de\u8840\u6e05\u304a\u3088\u30732.5% FBS<\/td>\n<\/tr>\n
IM-9<\/td>\n\u30ea\u30f3\u30d1\u82bd\u7403\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u9aa8\u9ac4\u816b\u60a3\u8005\u306e\u9aa8\u9ac4<\/td>\nRPMI-1640\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
JEG-2<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u7d68\u6bdb\u764c<\/td>\nMEM\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
Jensen<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30e9\u30c3\u30c8<\/td>\n\u8089\u816b<\/td>\nMcCoy’s 5A\u304a\u3088\u30735% FBS<\/td>\n<\/tr>\n
Jurkat<\/td>\n\u30ea\u30f3\u30d1\u82bd\u7403\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u30ea\u30f3\u30d1\u816b<\/td>\nRPMI-1640\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
K-562<\/td>\n\u30ea\u30f3\u30d1\u82bd\u7403\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u9aa8\u9ac4\u6027\u767d\u8840\u75c5<\/td>\nRPMI-1640\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
KB<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u53e3\u8154\u764c<\/td>\nMEM\u304a\u3088\u307310% FBS\u304a\u3088\u3073NEAA<\/td>\n<\/tr>\n
KG-1<\/td>\n\u9aa8\u9ac4\u82bd\u7403\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u8d64\u767d\u8840\u75c5\u60a3\u8005\u306e\u9aa8\u9ac4<\/td>\nIMDM\u304a\u3088\u307320% FBS<\/td>\n<\/tr>\n
L2<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30e9\u30c3\u30c8<\/td>\n\u80ba<\/td>\nF-12K\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
LLC-WRC 256<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30e9\u30c3\u30c8<\/td>\n\u764c<\/td>\nMedium 199\u304a\u3088\u30735% \u30a6\u30de\u8840\u6e05<\/td>\n<\/tr>\n
McCoy<\/td>\n\u7dda\u7dad\u82bd\u7d30\u80de<\/td>\n\u30de\u30a6\u30b9<\/td>\n\u672a\u77e5<\/td>\nMEM\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
MCF7<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u4e73\u817a\u764c<\/td>\nMEM\u304a\u3088\u307310% FBS\u304a\u3088\u3073NEAA\u304a\u3088\u307310 \u00b5g\/mL \u30a4\u30f3\u30b7\u30e5\u30ea\u30f3<\/td>\n<\/tr>\n
WI-38<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u80ce\u5150\u80ba<\/td>\nBME\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
WISH<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30d2\u30c8<\/td>\n\u7f8a\u819c<\/td>\nBME\u304a\u3088\u307310% FBS<\/td>\n<\/tr>\n
XC<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30e9\u30c3\u30c8<\/td>\n\u8089\u816b<\/td>\nMEM\u304a\u3088\u307310% FBS\u304a\u3088\u3073NEAA<\/td>\n<\/tr>\n
Y-1<\/td>\n\u4e0a\u76ae\u6027\u7d30\u80de<\/td>\n\u30de\u30a6\u30b9<\/td>\n\u526f\u814e\u816b\u760d<\/td>\nF-10, 15% \u30a6\u30de\u8840\u6e05\u304a\u3088\u30732.5% FBS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n
\u88682 \u6606\u866b\u57f9\u990a\u7d30\u80de\u306b\u9069\u3057\u305f\u57f9\u5730\u307e\u3068\u3081<\/caption>\n
\u6606\u866b\u7d30\u80de\u57f9\u990a<\/strong><\/th>\n<\/tr>\n
\u7d30\u80de\u7cfb<\/strong><\/th>\n\u7a2e<\/strong><\/th>\n\u7d44\u7e54<\/strong><\/th>\n\u57f9\u5730\u203b<\/sup><\/strong><\/th>\n<\/tr>\n
Sf9, Sf21<\/td>\n\u30c4\u30de\u30b8\u30ed\u30af\u30b5\u30e8\u30c8\u30a6
\n(Spodoptera frugiperda<\/i>)<\/td>\n		\u86f9\u306e\u5375\u5de3<\/td>\nTNM-FH\u304a\u3088\u307310% FBS\u307e\u305f\u306fSf-900 II SFM (\u7121\u8840\u6e05)\u307e\u305f\u306fSf-900\u2122 III SFM (\u7121\u8840\u6e05)<\/td>\n<\/tr>\n
High Five\u2122 (BTI-TN-5B1-4)<\/td>\n\u30ad\u30e3\u30d9\u30c4\u30b7\u30e3\u30af\u30c8\u30ea\u30e0\u30b7
\n(Trichoplusia ni<\/i>)<\/td>\n		\u5375\u5de3<\/td>\nTNM-FH\u304a\u3088\u307310% FBS\u307e\u305f\u306fExpress Five\u00ae SFM (\u7121\u8840\u6e05)<\/td>\n<\/tr>\n
Schneider 2 (S2), D.Mel-2<\/td>\n\u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8
\n(Drosophila melanogaster<\/i>)<\/td>\n		<\/td>\n\u30b7\u30e5\u30ca\u30a4\u30c0\u30fc\u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8\u57f9\u5730\u304a\u3088\u307310%\u975e\u50cd\u5316FBS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203bBME<\/strong>\uff1a Basal Medium Eagle\uff08\u30a4\u30fc\u30b0\u30eb\u57fa\u790e\u57f9\u5730\uff09\u3001DMEM<\/strong>\uff1a Dulbecco’s Modified Eagle Medium\uff08\u30c0\u30eb\u30d9\u30c3\u30b3\u5909\u6cd5\u30a4\u30fc\u30b0\u30eb\u57f9\u5730\uff09\u3001FBS<\/strong>\uff1a Fetal Bovine Serum\uff08\u30a6\u30b7\u80ce\u5150\u8840\u6e05\uff09\u3001GMEM<\/strong>\uff1a Glasgow Minimum Essential Medium\uff08\u30b0\u30e9\u30b9\u30b4\u30fc\u6700\u5c0f\u5fc5\u9808\u57f9\u5730\uff09\u3001IMDM<\/strong>\uff1a Iscove’s Modified Dulbecco’s Medium\uff08\u30a4\u30b9\u30b3\u30d5\u6539\u5909\u30c0\u30eb\u30d9\u30c3\u30b3\u57f9\u5730\uff09\u3001MEM<\/strong>\uff1a Minimum <\/span>Essential Medium\uff08\u6700\u5c0f\u5fc5\u9808\u57f9\u5730\uff09\u3001NEAA<\/strong>\uff1a Non-Essential Amino Acids Solution\uff08\u975e\u5fc5\u9808\u30a2\u30df\u30ce\u9178\u6eb6\u6db2\uff09\u3001TNM-FH<\/strong>\uff1a Trichoplusia ni Medium-Formulation Hink\uff08\u30b5\u30d7\u30ea\u30e1\u30f3\u30c8\u3092\u6dfb\u52a0\u3057\u305f\u30b0\u30ec\u30fc\u30b9\u6606\u866b\u57f9\u5730\uff09<\/span><\/p>\n

\u63a5\u7740\u7d30\u80de\u306e\u89e3\u96e2<\/h2>\n

\u63a5\u7740\u7d30\u80de\u306e\u7d99\u4ee3\u306e\u7b2c1\u6bb5\u968e\u306f\u3001\u7d30\u80de\u3092\u57f9\u990a\u5bb9\u5668\u304b\u3089\u3001\u9175\u7d20\u307e\u305f\u306f\u6a5f\u68b0\u7684\u306a\u65b9\u6cd5\u3067\u5265\u96e2\u3059\u308b\u3053\u3068\u3067\u3059\u3002\u69d8\u3005\u306a\u7d30\u80de\u89e3\u96e2\u306e\u65b9\u6cd5\u3092\u4e0b\u8868\u306b\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
\u88683 \u69d8\u3005\u306a\u7d30\u80de\u89e3\u96e2\u306e\u65b9\u6cd5<\/caption>\n
\u65b9\u6cd5<\/strong><\/td>\n\u89e3\u96e2\u5264<\/strong><\/td>\n\u9069\u5fdc\u5bfe\u8c61<\/strong><\/td>\n<\/tr>\n
\u632f\u308a\u843d\u3068\u3057<\/td>\n\u57f9\u990a\u5bb9\u5668\u306e\u7a4f\u3084\u304b\u306a\u632f\u308a\u6df7\u305c\u3001\u307e\u305f\u306f\u6fc0\u3057\u3044\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0<\/td>\n\u63a5\u7740\u6027\u306e\u5f31\u3044\u7d30\u80de\u304a\u3088\u3073\u6709\u7cf8\u5206\u88c2\u7d30\u80de<\/td>\n<\/tr>\n
\u30b9\u30af\u30ec\u30fc\u30d4\u30f3\u30b0<\/td>\n\u7d30\u80de\u30b9\u30af\u30ec\u30fc\u30d1\u30fc<\/td>\n\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u306e\u9ad8\u3044\u7d30\u80de\u7cfb\u3002\u7d30\u80de\u3092\u640d\u50b7\u3059\u308b\u53ef\u80fd\u6027\u3082\u3042\u308a\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u9175\u7d20\u7684\u89e3\u96e2<\/td>\n\u30c8\u30ea\u30d7\u30b7\u30f3<\/td>\n\u63a5\u7740\u6027\u306e\u9ad8\u3044\u7d30\u80de<\/td>\n<\/tr>\n
\u30c8\u30ea\u30d7\u30b7\u30f3+\u30b3\u30e9\u30b2\u30ca\u30fc\u30bc<\/td>\n\u9ad8\u5bc6\u5ea6\u306e\u57f9\u990a\u7d30\u80de\u3001\u8907\u6570\u306e\u5c64\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u7d30\u80de\u3001\u7279\u306b\u7dda\u7dad\u82bd\u7d30\u80de\u3002<\/td>\n<\/tr>\n
\u30c7\u30a3\u30b9\u30d1\u30fc\u30bc<\/td>\n\u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\u306b\u9054\u3057\u305f\u4e0a\u76ae\u7d30\u80de\u306e\u5206\u96e2\u3002\u57f9\u990a\u76bf\u306e\u8868\u9762\u304b\u3089\u3001\u7d30\u80de\u3092\u89e3\u96e2\u3059\u308b\u3053\u3068\u306a\u304f\u3001\u7d30\u80de\u30b7\u30fc\u30c8\u3068\u3057\u3066\u5206\u96e2\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n
TrypLE\u2122 \u89e3\u96e2\u7528\u9175\u7d20<\/td>\n\u63a5\u7740\u6027\u306e\u5f37\u3044\u7d30\u80de\u3002\u30c8\u30ea\u30d7\u30b7\u30f3\u306e\u4ee3\u7528\u3002\u52d5\u7269\u7531\u6765\u3067\u306a\u3044\u8a66\u85ac\u3092\u5fc5\u8981\u3068\u3059\u308b\u5834\u5408\u306b\u4f7f\u7528\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

TrypLE\u89e3\u96e2\u7528\u9175\u7d20<\/h2>\n

Gibco\u2122 TrypLE\u2122 Express\u304a\u3088\u3073Gibco\u2122 TrypLE\u2122 Select<\/a>\u306f\u3001\u5fae\u751f\u7269\u751f\u7523\u3055\u308c\u305f\u7d30\u80de\u89e3\u96e2\u7528\u9175\u7d20\u3067\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u3068\u540c\u69d8\u306e\u52d5\u614b\u304a\u3088\u3073\u5207\u65ad\u7279\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u7d30\u80de\u306e\u89e3\u96e2\u306b\u304a\u3044\u3066\u306f\u3001\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306e\u5909\u66f4\u3092\u884c\u3046\u3053\u3068\u306a\u304f\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u3092\u76f4\u63a5TrypLE\u9175\u7d20\u3067\u7f6e\u304d\u63db\u3048\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u304c\u3001\u6700\u826f\u306e\u7d50\u679c\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\u3001\u307e\u305a\u89e3\u96e2\u306e\u305f\u3081\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u6642\u9593\u306e\u6700\u9069\u5316\u3092\u884c\u308f\u308c\u308b\u3053\u3068\u3092\u63a8\u5968\u3057\u307e\u3059\u3002<\/p>\n

TrypLE\u9175\u7d20\u306f\u3001\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u306e\u771f\u83cc\u6027\u30c8\u30ea\u30d7\u30b7\u30f3\u69d8\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u3067\u3059\u306e\u3067\u3001\u52d5\u7269\u7531\u6765\u3067\u306a\u3044\u8a66\u85ac\u3092\u5fc5\u8981\u3068\u3059\u308b\u5b9f\u9a13\u306b\u306f\u7406\u60f3\u7684\u3067\u3059\u3002TrypLE Express\u304a\u3088\u3073TrypLE Select\u3068\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u3068\u306e\u6bd4\u8f03\u3092\u4e0b\u8868\u306b\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n\n\n\n\n
\u88684 TrypLE Express\u304a\u3088\u3073TrypLE Select\u3068\u30c8\u30ea\u30d7\u30b7\u30f3\u3068\u306e\u6bd4\u8f03<\/caption>\n
TrypLE Express\u304a\u3088\u3073TrypLE Select<\/strong><\/td>\n\u30c8\u30ea\u30d7\u30b7\u30f3<\/strong><\/td>\n<\/tr>\n
\u52d5\u7269\u304a\u3088\u3073\u30d2\u30c8\u7531\u6765\u306e\u6210\u5206\u306f\u3001\u4e00\u5207\u4e0d\u542b<\/td>\n\u30d6\u30bf\u307e\u305f\u306f\u30a6\u30b7\u7531\u6765<\/td>\n<\/tr>\n
\u5ba4\u6e29\u3067\u6700\u4f4e6\u30f5\u6708\u306f\u5b89\u5b9a<\/td>\n\u5ba4\u6e29\u3067\u306f\u4e0d\u5b89\u5b9a<\/td>\n<\/tr>\n
\u4e0d\u6d3b\u5316\u306e\u5fc5\u8981\u306a\u3057<\/td>\n\u8840\u6e05\u307e\u305f\u306f\u305d\u306e\u4ed6\u306e\u963b\u5bb3\u5264\u306b\u3088\u308b\u4e0d\u6d3b\u5316\u304c\u5fc5\u8981<\/td>\n<\/tr>\n
\u30c8\u30ea\u30d7\u30b7\u30f3\u4e0d\u6d3b\u5316\u5264\u3092\u5fc5\u8981\u306a\u3057<\/td>\n\u30c8\u30ea\u30d7\u30b7\u30f3\u4e0d\u6d3b\u5316\u5264\u304c\u5fc5\u8981<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011Gibco\u7d30\u80de\u57f9\u990a\u57fa\u790e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n

\u7d30\u80de\u57f9\u990a\u306b\u95a2\u3059\u308b\u57fa\u790e\u60c5\u5831\u3092\u89e3\u8aac\u3057\u305f\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\uff08\u65e5\u672c\u8a9e\u7248\u3001\u7d04100\u30da\u30fc\u30b8\uff09\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/a><\/p>\n

 <\/p>\n

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