{"id":534,"date":"2016-12-19T00:00:00","date_gmt":"2016-12-19T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/"},"modified":"2025-02-12T15:19:50","modified_gmt":"2025-02-12T06:19:50","slug":"basic-of-transfection","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/","title":{"rendered":"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58"},"content":{"rendered":"

\u6a5f\u80fd\u89e3\u6790\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3084\u751f\u7523\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u6a5f\u80fd\u306e\u963b\u5bb3\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u5b9f\u9a13\u3067\u7d30\u80de\u3078\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u304c\u5fc5\u8981\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u306b\u306f\u3001\u7d30\u80de\u306b\u9069\u3057\u305f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306e\u9078\u629e\u304c\u5fc5\u8981\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u57fa\u790e\u77e5\u8b58\u3068\u3057\u3066\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u6982\u8981\u3068\u7a2e\u985e\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/strong><\/p>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6982\u8ad6<\/h2>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3068\u306f?<\/h3>\n

\u5e83\u7fa9\u3067\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3068\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u4ee5\u5916\u306e\u65b9\u6cd5\u3067\u6838\u9178\uff08DNA\u307e\u305f\u306fRNA\uff09\u3092\u7d30\u80de\u306b\u4eba\u70ba\u7684\u306b\u5c0e\u5165\u3059\u308b\u30d7\u30ed\u30bb\u30b9\u3092\u610f\u5473\u3057\u307e\u3059\u3002<\/strong>\u3053\u306e\u3088\u3046\u306a\u5916\u6765\u6838\u9178\u306e\u5c0e\u5165\u306b\u306f\u69d8\u3005\u306a\u5316\u5b66\u7684\u624b\u6cd5\u3001\u751f\u7269\u5b66\u7684\u624b\u6cd5\u3001\u307e\u305f\u306f\u7269\u7406\u7684\u624b\u6cd5\u304c\u7528\u3044\u3089\u308c\u3001\u305d\u308c\u306b\u3088\u3063\u3066\u7d30\u80de\u306e\u7279\u6027\u304c\u5909\u5316\u3059\u308b\u3053\u3068\u3067\u3001\u907a\u4f1d\u5b50\u6a5f\u80fd\u304a\u3088\u3073\u7d30\u80de\u5185\u306b\u304a\u3051\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306b\u95a2\u3059\u308b\u7814\u7a76\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u306f\u3001\u5c0e\u5165\u3055\u308c\u305f\u6838\u9178\u306f\u7d30\u80de\u5185\u306b\u4e00\u904e\u7684\u306b\u5b58\u5728\u3057\u3001\u9650\u3089\u308c\u305f\u671f\u9593\u306e\u307f\u767a\u73fe\u3057\u8907\u88fd\u3055\u308c\u306a\u3044\u30bf\u30a4\u30d7<\/strong>\u3068\u3001\u5c0e\u5165\u3055\u308c\u305f\u6838\u9178\u306f\u5b89\u5b9a\u3067 \u3001\u5bbf\u4e3b\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u307e\u308c\u5bbf\u4e3b\u306e\u30b2\u30ce\u30e0\u304c\u8907\u88fd\u3055\u308c\u308b\u969b\u306b\u8907\u88fd\u3055\u308c\u308b\u30bf\u30a4\u30d7<\/strong>\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u7528\u8a9e<\/h3>\n

\u69d8\u3005\u306a\u907a\u4f1d\u5b50\u5c0e\u5165\u30b7\u30b9\u30c6\u30e0\u3067\u4f7f\u7528\u3055\u308c\u308b\u7528\u8a9e\u306f\u3001\u3053\u306e\u5206\u91ce\u306b\u304a\u3051\u308b\u6280\u8853\u306e\u9032\u6b69\u3068\u3068\u3082\u306b\u9032\u5316\u3057\u3001\u69d8\u3005\u306a\u624b\u6cd5\u3068\u7d30\u80de\u578b\u3092\u8b58\u5225\u3059\u308b\u305f\u3081\u306b\u3055\u3089\u306b\u7d30\u5206\u5316\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3<\/h4>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3068\u306f\u3001\u901a\u5e38\u3001\u6838\u9178\u3092\u771f\u6838\u7d30\u80de\u306b\u3001\u3055\u3089\u306b\u5177\u4f53\u7684\u306b\u306f\u52d5\u7269\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u3092\u610f\u5473\u3057\u307e\u3059\u3002<\/strong>\u5f93\u6765\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3068\u3044\u3046\u7528\u8a9e\u306f\u3001\u539f\u6838\u751f\u7269\u306b\u611f\u67d3\u3057\u305f\u30a6\u30a4\u30eb\u30b9\u3001\u3059\u306a\u308f\u3061\u30d0\u30af\u30c6\u30ea\u30aa\u30d5\u30a1\u30fc\u30b8\u7531\u6765\u306e\u30a6\u30a4\u30eb\u30b9\u6838\u9178\u304c\u53d6\u308a\u8fbc\u307e\u308c\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u611f\u67d3\u304a\u3088\u3073\u6210\u719f\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u306e\u5f62\u6210\u304c\u751f\u3058\u308b\u3053\u3068\u3092\u610f\u5473\u3057\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u73fe\u5728\u3067\u306f\u3001\u3053\u306e\u7528\u8a9e\u306f\u3042\u3089\u3086\u308b\u4eba\u70ba\u7684\u306a\u624b\u6cd5\u3092\u542b\u3080\u3001\u5916\u6765\u6838\u9178\u306e\u7d30\u80de\u3078\u306e\u5c0e\u5165\u3092\u610f\u5473\u3059\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002<\/strong><\/p>\n

\u5f62\u8cea\u8ee2\u63db<\/h4>\n

\u5f62\u8cea\u8ee2\u63db\u3068\u306f\u3001\u591a\u304f\u306e\u5834\u5408\u3001\u975e\u30a6\u30a4\u30eb\u30b9DNA\u3092\u7d30\u83cc\u3001\u975e\u52d5\u7269\u771f\u6838\u7d30\u80de\u3001\u304a\u3088\u3073\u690d\u7269\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u3092\u610f\u5473\u3057\u307e\u3059\u3002<\/strong>\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u5f62\u8cea\u8ee2\u63db\u3068\u3044\u3046\u7528\u8a9e\u306f\u3001\u52d5\u7269\u7d30\u80de\u306e\u8868\u73fe\u578b\u306b\u6c38\u4e45\u7684\u306a\u5909\u5316\u3092\u3082\u305f\u3089\u3059\u7279\u5b9a\u306e\u30a4\u30d9\u30f3\u30c8\u3084\u4e00\u9023\u306e\u30a4\u30d9\u30f3\u30c8\u3082\u610f\u5473\u3057\u3001\u907a\u4f1d\u5b50\u7684\u306b\u4e0d\u5b89\u5b9a\u3067\u3001\u764c\u5316\u3057\u3084\u3059\u3044\u72b6\u614b\u306b\u3042\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u307e\u3059\u3002\u3053\u306e\u610f\u5473\u3067\u306e\u5f62\u8cea\u8ee2\u63db\u306f\u3001\u5f62\u8cea\u8ee2\u63db\u30a6\u30a4\u30eb\u30b9\u306b\u3088\u308b\u611f\u67d3\u3084\u907a\u4f1d\u5b50\u5c0e\u5165\u306b\u3088\u3063\u3066\u8d77\u3053\u308a\u5f97\u307e\u3059\u304c\u3001\u81ea\u7136\u767a\u751f\u7684\u306b\u3082\u8d77\u304d\u3001\u3055\u3089\u306b\u3001\u96fb\u96e2\u653e\u5c04\u7dda\u3084\u5316\u5b66\u5909\u7570\u539f\u306a\u3069\u306e\u5916\u90e8\u30b9\u30c8\u30ec\u30c3\u30b5\u30fc\u306b\u3088\u3063\u3066\u3082\u751f\u3058\u5f97\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u3053\u306e\u7528\u8a9e\u306f\u52d5\u7269\u7d30\u80de\u306b\u5916\u6765\u907a\u4f1d\u7269\u8cea\u3092\u5c0e\u5165\u3059\u308b\u3053\u3068\u3092\u793a\u3059\u8868\u73fe\u3068\u3057\u3066\u306f\u7528\u3044\u3089\u308c\u307e\u305b\u3093\u3002<\/strong><\/p>\n

\u5f62\u8cea\u5c0e\u5165<\/h4>\n

\u5f62\u8cea\u5c0e\u5165\u3068\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u3092\u4ecb\u3059\u308bDNA\u306e\u5c0e\u5165\u3092\u610f\u5473\u3057\u307e\u3059\u3002<\/strong>\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3068\u3044\u3046\u7528\u8a9e\u3082\u771f\u6838\u751f\u7269\u30a6\u30a4\u30eb\u30b9\u307e\u305f\u306f\u30d0\u30af\u30c6\u30ea\u30aa\u30d5\u30a1\u30fc\u30b8\u304b\u3089\u5358\u96e2\u3055\u308c\u305f\u30a6\u30a4\u30eb\u30b9\u6838\u9178\u3092\u7279\u7570\u7684\u306b\u7d30\u80de\u306b\u611f\u67d3\u3055\u305b\u308b\u3068\u3044\u3046\u610f\u5473\u3067\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/h3>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e2\u3064\u306e\u4e3b\u8981\u306a\u76ee\u7684\u306f\u3001\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7523\u751f\u3059\u308b\u3053\u3068<\/strong>\u3068\u3001\u5c0e\u5165\u7d30\u80de\u306b\u304a\u3051\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u7279\u7570\u7684\u306b\u5897\u5f37\u307e\u305f\u306f\u6291\u5236\u3059\u308b\u3053\u3068<\/strong>\u3067\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u907a\u4f1d\u5b50\u3084\u907a\u4f1d\u5b50\u7523\u7269\u306e\u6a5f\u80fd\u304a\u3088\u3073\u8abf\u7bc0\u306b\u95a2\u3059\u308b\u7814\u7a76\u3084\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u751f\u7269\u306e\u4f5c\u88fd\u306b\u95a2\u3059\u308b\u7814\u7a76\u306e\u305f\u3081\u306e\u5f37\u529b\u306a\u5206\u6790\u30c4\u30fc\u30eb\u3067\u3042\u308a\u3001\u907a\u4f1d\u5b50\u7642\u6cd5\u306e\u65b9\u6cd5\u3067\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u907a\u4f1d\u5b50\u767a\u73fe<\/h4>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u307e\u305f\u306fmRNA\u3092\u4f7f\u7528\u3057\u3066\u57f9\u990a\u7d30\u80de\uff08\u307e\u305f\u306f\u52d5\u7269\u30e2\u30c7\u30eb\uff09\u3067\u306e\u76ee\u7684\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u767a\u73fe\u3055\u305b\u308b\u306e\u306b\u6700\u3082\u3088\u304f\u5b9f\u65bd\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/strong>\u771f\u6838\u7d30\u80de\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u767a\u73fe\u3055\u305b\u308b\u3068\u3001\u9069\u5207\u306a\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3068\u305d\u306e\u6a5f\u80fd\u3067\u5fc5\u8981\u3068\u3055\u308c\u308b\u7ffb\u8a33\u5f8c\u4fee\u98fe\u3092\u53d7\u3051\u305f\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u5bb9\u6613\u306b\u691c\u51fa\u53ef\u80fd\u306a\u30de\u30fc\u30ab\u30fc\u304a\u3088\u3073\u4ed6\u306e\u4fee\u98fe\u3092\u65bd\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u914d\u5217\u304a\u3088\u3073\u30a8\u30f3\u30cf\u30f3\u30b5\u30fc\u914d\u5217\u3042\u308b\u3044\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u306b\u95a2\u3059\u308b\u7814\u7a76\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u307e\u305f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u65b9\u6cd5\u306b\u5fdc\u3058\u3066\u69d8\u3005\u306a\u5f62\u614b\u306e\u30d0\u30a4\u30aa\u751f\u7523\u306b\u3082\u7528\u3044\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u30ea\u30d7\u30ed\u30b0\u30e9\u30df\u30f3\u30b0\u8ee2\u5199\u56e0\u5b50\u306e\u5c0e\u5165\u306f\u3001\u4eba\u5de5\u591a\u80fd\u6027\u5e79\u7d30\u80de\uff08iPSC\uff09\u306e\u4f5c\u88fd\u3092\u53ef\u80fd\u3068\u3057\u307e\u3059\u3002\u4e00\u65b9\u3001\u5b89\u5b9a\u306a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u69d8\u3005\u306a\u6cbb\u7642\u7528\u5206\u5b50\u306e\u30d0\u30a4\u30aa\u751f\u7523\u306e\u624b\u6cd5\u3068\u3057\u3066\u63d0\u4f9b\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u907a\u4f1d\u5b50\u963b\u5bb3<\/h4>\n

\u3082\u30461\u3064\u306e\u3088\u304f\u7528\u3044\u3089\u308c\u308b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u65b9\u6cd5\u306b\u3001RNA\u5e72\u6e09\uff08RNAi\uff09\u3092\u4ecb\u3059\u308b\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306e\u963b\u5bb3\u304c\u3042\u308a\u307e\u3059\u3002<\/strong>\u54fa\u4e73\u985e\u7d30\u80de\u3067\u306f\u3001\u4e8c\u672c\u9396RNA\uff08dsRNA\uff09\u524d\u99c6\u4f53\u304b\u3089\u751f\u3058\u308b\u30de\u30a4\u30af\u30edRNA\uff08miRNA\uff09\u306e\u5f62\u614b\u3067\u5185\u56e0\u7684\u306b\u767a\u73fe\u3055\u308c\u305f\u30ce\u30f3\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0RNA\u3092\u4ecb\u3057\u3066 \u3001RNAi\u52b9\u679c\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u3053\u306e\u524d\u99c6\u4f53\u306f\u3001\u30d7\u30ed\u30bb\u30b7\u30f3\u30b0\u3055\u308c\u3066\u6210\u719fmiRNA\u3068\u306a\u308a\u3001RNA\u8a98\u5c0e\u30b5\u30a4\u30ec\u30f3\u30b7\u30f3\u30b0\u8907\u5408\u4f53\uff08RISC\uff09\u306b\u7d44\u307f\u8fbc\u307e\u308c\u3066\u305d\u306e\u4e00\u90e8\u3068\u306a\u308a\u3001\u76f8\u88dc\u7684\u306a\u6a19\u7684mRNA\u306e\u7ffb\u8a33\u3092\u963b\u5bb3\u3059\u308b\u3088\u3046\u306b\u4f5c\u7528\u3057\u307e\u3059\u3002<\/p>\n

\u30d9\u30af\u30bf\u30fc\u30d9\u30fc\u30b9\u306e\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u3063\u3066\u767a\u73fe\u3057\u305fmiRNA\u524d\u99c6\u4f53\u307e\u305f\u306f\u77ed\u3044\u30d8\u30a2\u30d4\u30f3RNA\uff08shRNA\uff09\u524d\u99c6\u4f53\u306f\u3001\u7d30\u80de\u5185\u6a5f\u69cb\u306b\u3088\u3063\u3066\u30d7\u30ed\u30bb\u30b7\u30f3\u30b0\u3055\u308c\u3001\u305d\u308c\u305e\u308cmiRNA\u307e\u305f\u306fshRNA\u3092\u751f\u3058\u3001\u305d\u308c\u3089\u306f\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u963b\u5bb3\u3059\u308b\u3088\u3046\u306b\u4f5c\u7528\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u7d44\u63db\u3048\u30b3\u30f3\u30b9\u30c8\u30e9\u30af\u30c8\u306e\u5b89\u5b9a\u7684\u306a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3092\u53ef\u80fd\u3068\u3057\u3001\u524d\u99c6\u4f53\u5206\u5b50\u306e\u8a98\u5c0e\u767a\u73fe\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002<\/p>\n

\u5316\u5b66\u5408\u6210\u3055\u308c\u305f\u77ed\u3044\uff0f\u5c0f\u3055\u3044\u5e72\u6e09RNA\uff08siRNA\uff09\u3082RISC\u306b\u7d44\u307f\u8fbc\u307e\u308c\u3001\u76f8\u88dc\u7684\u306a\u6a19\u7684mRNA\u3092\u5206\u89e3\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u907a\u4f1d\u5b50\u30b5\u30a4\u30ec\u30f3\u30b7\u30f3\u30b0\u3092\u8a98\u5c0e\u3057\u307e\u3059\u3002siRNA\u306b\u4fee\u98fe\u3092\u65bd\u3059\u3053\u3068\u3067\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u6291\u3048\u3001dsRNA\u306e\u6d3b\u6027\u9396\u3092RISC\u306b\u7d44\u307f\u8fbc\u3080\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u7a2e\u985e<\/h2>\n

\u6838\u9178\u3092\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u6570\u591a\u304f\u306e\u751f\u7269\u5b66\u7684\u3001\u5316\u5b66\u7684\u3001\u304a\u3088\u3073\u7269\u7406\u7684\u306a\u624b\u6cd5\u304c\u5b58\u5728\u3057\u307e\u3059\u3002<\/strong>\u3053\u308c\u3089\u306e\u5168\u3066\u306e\u624b\u6cd5\u304c\u5168\u3066\u306e\u7d30\u80de\u578b\u304a\u3088\u3073\u5b9f\u9a13\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u9069\u5408\u3059\u308b\u308f\u3051\u3067\u306f\u306a\u304f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3001\u7d30\u80de\u6bd2\u6027\u3001\u6b63\u5e38\u306a\u751f\u7406\u6a5f\u80fd\u3078\u306e\u5f71\u97ff\u3001\u304a\u3088\u3073\u907a\u4f1d\u5b50\u767a\u73fe\u30ec\u30d9\u30eb\u306b\u5fdc\u3058\u3066\u5bfe\u5fdc\u3059\u308b\u5e45\u5e83\u3044\u30d0\u30ea\u30a8\u30fc\u30b7\u30e7\u30f3\u304c\u5b58\u5728\u3057\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u5168\u3066\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6226\u7565\u306f\u3001\u5c0e\u5165\u3055\u308c\u305f\u6838\u9178\u304c\u7d30\u80de\u5185\u306b\u5b58\u5728\u3059\u308b\u306e\u304c\u9650\u3089\u308c\u305f\u671f\u9593\u3067\u3042\u308b\u304b\uff08\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3<\/strong>\uff09\u3001\u3042\u308b\u3044\u306f\u9577\u671f\u9593\u306b\u308f\u305f\u308a\u7d30\u80de\u5185\u306b\u5b58\u7d9a\u3057\u3001\u5c0e\u5165\u7d30\u80de\u306e\u5b50\u5b6b\u306b\u5f15\u304d\u7d99\u304c\u308c\u308b\u304b\uff08\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3<\/strong>\uff09\u306e\u5927\u304d\u304f2\u3064\u306b\u5206\u985e\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3<\/h3>\n

\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3067\u306f\u3001\u5c0e\u5165\u3055\u308c\u305f\u6838\u9178\u306f\u9650\u3089\u308c\u305f\u671f\u9593\u3060\u3051\u7d30\u80de\u5185\u306b\u5b58\u5728\u3057\u3001\u30b2\u30ce\u30e0\u306b\u306f\u7d44\u307f\u8fbc\u307e\u308c\u307e\u305b\u3093\u3002<\/strong>\u3053\u306e\u3088\u3046\u306b\u4e00\u904e\u7684\u306b\u5c0e\u5165\u3055\u308c\u305f\u907a\u4f1d\u7269\u8cea\u306f\u7d30\u80de\u5206\u88c2\u3092\u4ecb\u3057\u3066\u4e16\u4ee3\u304b\u3089\u4e16\u4ee3\u3078\u3068\u53d7\u3051\u7d99\u304c\u308c\u308b\u3053\u3068\u306f\u306a\u304f\u3001\u74b0\u5883\u56e0\u5b50\u306b\u3088\u3063\u3066\u5931\u308f\u308c\u308b\u304b\u3001\u7d30\u80de\u5206\u88c2\u3092\u901a\u3057\u3066\u5e0c\u91c8\u3055\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u9ad8\u30b3\u30d4\u30fc\u6570\u3067\u5c0e\u5165\u3055\u308c\u308b\u907a\u4f1d\u7269\u8cea\u306f\u3001\u305d\u306e\u671f\u9593\u3001\u9ad8\u30ec\u30d9\u30eb\u306e\u767a\u73fe\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7d30\u80de\u5185\u306b\u5b58\u5728\u3055\u305b\u308b\u3053\u3068\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002<\/strong><\/p>\n

\u4f7f\u7528\u3059\u308b\u30b3\u30f3\u30b9\u30c8\u30e9\u30af\u30c8\u306b\u3088\u308a\u307e\u3059\u304c\u3001\u4e00\u904e\u7684\u306b\u767a\u73fe\u3055\u308c\u308b\u5c0e\u5165\u907a\u4f1d\u5b50\u306f\u4e00\u822c\u7684\u306b1\uff5e7\u65e5\u9593\u306b\u308f\u305f\u308a\u691c\u51fa\u3055\u308c\u3001\u4e00\u904e\u7684\u306b\u5c0e\u5165\u3055\u308c\u305f\u7d30\u80de\u306f\u901a\u5e38\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5f8c24\uff5e96\u6642\u9593\u4ee5\u5185\u306b\u56de\u53ce\u3055\u308c\u307e\u3059\u3002\u907a\u4f1d\u5b50\u7523\u7269\u3092\u89e3\u6790\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u9175\u7d20\u6d3b\u6027\u30a2\u30c3\u30bb\u30a4\u3084\u514d\u75ab\u30a2\u30c3\u30bb\u30a4\u306b\u4f7f\u7528\u3059\u308b\u305f\u3081\u306eRNA\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u96e2\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u6700\u9069\u306a\u671f\u9593\u306e\u9593\u9694\u306f\u3001\u7d30\u80de\u306e\u7a2e\u985e\u3001\u7814\u7a76\u76ee\u6a19\u3001\u304a\u3088\u3073\u5c0e\u5165\u3055\u308c\u305f\u907a\u4f1d\u5b50\u7279\u6709\u306e\u767a\u73fe\u7279\u6027\u3001\u306a\u3089\u3073\u306b\u30ec\u30dd\u30fc\u30bf\u30fc\u304c\u5b89\u5b9a\u306a\u72b6\u614b\u306b\u5230\u9054\u3059\u308b\u307e\u3067\u306e\u671f\u9593\u306b\u4f9d\u5b58\u3057\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u5927\u90e8\u5206\u306e\u5916\u6765DNA\u306f2\uff5e3\u65e5\u4ee5\u5185\u306b\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306b\u3088\u3063\u3066\u5206\u89e3\u3055\u308c\u308b\u304b\u3001\u7d30\u80de\u5206\u88c2\u306b\u3088\u3063\u3066\u5e0c\u91c8\u3055\u308c\u3001\u4e00\u9031\u9593\u5f8c\u306b\u306f\u305d\u306e\u5b58\u5728\u306f\u691c\u51fa\u3055\u308c\u306a\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u30b9\u30fc\u30d1\u30fc\u30b3\u30a4\u30eb\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u7528\u3044\u3089\u308c\u308b\u5834\u5408\u306b\u3001\u3088\u308a\u52b9\u7387\u7684\u306b\u7d30\u80de\u306b\u53d6\u308a\u8fbc\u307e\u308c\u308b\u3068\u60f3\u5b9a\u3055\u308c\u3001\u6700\u3082\u52b9\u7387\u7684\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/strong>siRNA\u3001miRNA\u3001mRNA\u306b\u52a0\u3048\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3055\u3048\u3082\u3001\u30d7\u30e9\u30b9\u30df\u30c9DNA\u3068\u540c\u69d8\u306b\u3001\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u7528\u3044\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u304c\u3001\u3053\u308c\u3089\u306e\u9ad8\u5206\u5b50\u306f\u9ad8\u54c1\u8cea\u3067\u6bd4\u8f03\u7684\u7d14\u7c8b\u3067\u3042\u308b\u3053\u3068\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002\u5c0e\u5165\u3055\u308c\u305fDNA\u306f\u6838\u306b\u79fb\u884c\u3057\u3001\u8ee2\u5199\u3055\u308c\u308b\u306e\u306b\u5bfe\u3057\u3001\u5c0e\u5165\u3055\u308c\u305fRNA\u306f\u30b5\u30a4\u30c8\u30be\u30eb\u306b\u6b8b\u308a\u3001\u8ee2\u5199\u5f8c\uff08mRNA\uff09\u307e\u305f\u306fmRNA\u3078\u306e\u7d50\u5408\u5f8c\u3001\u6570\u5206\u4ee5\u5185\u306b\u767a\u73fe\u3055\u308c\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\uff08siRNA\u304a\u3088\u3073miRNA\uff09\u306e\u767a\u73fe\u3092\u6291\u5236\u3057\u307e\u3059\u3002<\/p>\n

\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3<\/h3>\n

\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3067\u306f\u3001\u5916\u6765DNA\u306f\u7d30\u80de\u5185\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u307e\u308c\u308b\u304b\u3001\u30a8\u30d4\u30bd\u30fc\u30e0\u6027\u30d7\u30e9\u30b9\u30df\u30c9\u3068\u3057\u3066\u7dad\u6301\u3055\u308c\u307e\u3059\u3002<\/strong>\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3068\u306f\u7570\u306a\u308a\u3001\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u9577\u671f\u9593\u306b\u308f\u305f\u308a\u5916\u6765DNA\u3092\u5c0e\u5165\u7d30\u80de\u304a\u3088\u3073\u305d\u306e\u5b50\u5b6b\u306b\u7dad\u6301\u3059\u308b\u3053\u3068\u3092\u53ef\u80fd\u3068\u3057\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u8907\u6570\u4e16\u4ee3\u306b\u308f\u305f\u308a\u5c0e\u5165\u3055\u308c\u305f\u907a\u4f1d\u5b50\u3092\u6301\u7d9a\u7684\u306b\u767a\u73fe\u3055\u305b\u308b\u3053\u3068\u3092\u53ef\u80fd\u3068\u3057\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u751f\u7523\u3001\u3042\u308b\u3044\u306f\u5916\u6765DNA\u767a\u73fe\u306e\u4e0b\u6d41\u3084\u9577\u671f\u7684\u5f71\u97ff\u306e\u89e3\u6790\u306b\u6709\u7528\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u901a\u5e38\u3001\u5b89\u5b9a\u7684\u306b\u5c0e\u5165\u7d30\u80de\u306e\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u307e\u308c\u308b\u306e\u306f\u30011\u307e\u305f\u306f2\uff5e3\u30b3\u30d4\u30fc\u306e\u5916\u6765DNA\u3067\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u5b89\u5b9a\u7684\u306b\u5c0e\u5165\u3055\u308c\u305f\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u306f\u3001\u4e00\u904e\u7684\u306b\u5c0e\u5165\u3055\u308c\u305f\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u3088\u308a\u3082\u4f4e\u3044\u50be\u5411\u304c\u3042\u308a\u307e\u3059\u3002<\/strong><\/p>\n

\u5916\u6765DNA\u304c\u5b89\u5b9a\u7684\u306b\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u307e\u308c\u308b\u3053\u3068\u306f\u6bd4\u8f03\u7684\u7a00\u306a\u30a4\u30d9\u30f3\u30c8\u3067\u3042\u308b\u305f\u3081\u3001\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3092\u6210\u529f\u3055\u305b\u308b\u305f\u3081\u306b\u306f\u3001\u52b9\u7387\u7684\u306aDNA\u5c0e\u5165\u3068\u305d\u306eDNA\u3092\u53d6\u308a\u8fbc\u3093\u3060\u7d30\u80de\u3092\u9078\u629e\u3059\u308b\u65b9\u6cd5\u304c\u5fc5\u8981<\/strong>\u3068\u3055\u308c\u307e\u3059\u3002\u5c0e\u5165\u3055\u308c\u305fDNA\u304c\u5b89\u5b9a\u7684\u306b\u767a\u73fe\u3057\u3066\u3044\u308b\u7d30\u80de\u3092\u9078\u629e\u3059\u308b\u305f\u3081\u306e\u6700\u3082\u4fe1\u983c\u3067\u304d\u308b\u65b9\u6cd5\u306e1\u3064\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u7528\u3044\u3089\u308c\u308bDNA\u30b3\u30f3\u30b9\u30c8\u30e9\u30af\u30c8\u4e0a\u306b\u9078\u629e\u30de\u30fc\u30ab\u30fc\u3092\u542b\u307e\u305b\u3066\u3001\u77ed\u3044\u56de\u5fa9\u671f\u9593\u5f8c\u306b\u7d30\u80de\u306b\u9069\u5207\u306a\u9078\u629e\u5727\u3092\u304b\u3051\u308b\u3082\u306e\u3067\u3059\u3002<\/p>\n

\u983b\u7e41\u306b\u4f7f\u7528\u3055\u308c\u308b\u9078\u629e\u30de\u30fc\u30ab\u30fc\u306f\u3001\u69d8\u3005\u306a\u9078\u629e\u8a66\u85ac\u306b\u5bfe\u3059\u308b\u8010\u6027\u3092\u4e0e\u3048\u308b\u907a\u4f1d\u5b50\u3001\u3042\u308b\u3044\u306f\u5c0e\u5165\u3055\u308c\u308b\u7d30\u80de\u7cfb\u306b\u304a\u3044\u3066\u6b20\u640d\u3057\u3066\u3044\u308b\u5fc5\u9808\u907a\u4f1d\u5b50\u3092\u88dc\u3046\u907a\u4f1d\u5b50\u3067\u3059\u3002<\/strong>\u9078\u629e\u57f9\u5730\u3067\u57f9\u990a\u3059\u308b\u3068\u3001\u9078\u629e\u30de\u30fc\u30ab\u30fc\u304c\u5c0e\u5165\u3055\u308c\u3066\u3044\u306a\u3044\u7d30\u80de\u3042\u308b\u3044\u306f\u4e00\u904e\u7684\u306b\u5c0e\u5165\u3055\u308c\u305f\u7d30\u80de\u306f\u6700\u7d42\u7684\u306b\u6b7b\u6ec5\u3057\u3001\u6297\u751f\u7269\u8cea\u8010\u6027\u907a\u4f1d\u5b50\u3092\u5341\u5206\u306a\u30ec\u30d9\u30eb\u3067\u767a\u73fe\u3057\u3066\u3044\u308b\u7d30\u80de\u3042\u308b\u3044\u306f\u5fc5\u9808\u907a\u4f1d\u5b50\u306e\u6b20\u5931\u304c\u4ee3\u511f\u3055\u308c\u3066\u3044\u308b\u7d30\u80de\u306f\u751f\u5b58\u3057\u307e\u3059\u3002<\/p>\n

\u4ed6\u306e\u65b9\u6cd5\u3068\u3057\u3066\u306f\u3001\u7279\u5b9a\u306e\u30b1\u30fc\u30b9\u306b\u304a\u3044\u3066\u3001\u5c0e\u5165\u7d30\u80de\u306b\u304a\u3051\u308b\u8868\u73fe\u6027\u307e\u305f\u306f\u5f62\u614b\u306e\u5909\u5316\u3092\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u53ef\u80fd\u306a\u7279\u6027\u3068\u3057\u3066\u7528\u3044\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u30a6\u30b7\u30d1\u30d4\u30ed\u30fc\u30de\u30a6\u30a4\u30eb\u30b9\u30de\u30a6\u30b9\u7531\u6765\u306e\u30d9\u30af\u30bf\u30fc\u3092\u5c0e\u5165\u3057\u305f\u30de\u30a6\u30b9CI127\u7d30\u80de\u306f\u5f62\u614b\u5909\u5316\u3092\u751f\u3058\u307e\u3059\uff08Sarver et al.1981\uff09\u3002<\/p>\n

\u76f4\u9396\u72b6DNA\u306e\u7d30\u80de\u3078\u306eDNA\u53d6\u308a\u8fbc\u307f\u91cf\u306f\u3001\u30b9\u30fc\u30d1\u30fc\u30b3\u30a4\u30ebDNA\u306b\u6bd4\u3079\u3066\u4f4e\u3044\u3067\u3059\u304c\u3001DNA\u306e\u5bbf\u4e3b\u30b2\u30ce\u30e0\u3078\u306e\u6700\u9069\u306a\u7d44\u307f\u8fbc\u307f\u3092\u751f\u3058\u307e\u3059\u3002<\/strong>\u4e00\u822c\u306b\u3001\u5b89\u5b9a\u306a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306fDNA\u30d9\u30af\u30bf\u30fc\u306e\u5c0e\u5165\u306b\u9650\u5b9a\u3055\u308c\u307e\u3059\u304c\u3001siRNA\u304a\u3088\u3073miRNA\u306f\u9078\u629e\u53ef\u80fd\u306aDNA\u30d9\u30af\u30bf\u30fc\u304b\u3089\u4f5c\u88fd\u3055\u308c\u305f\u77ed\u3044\u30d8\u30a2\u30d4\u30f3\u8ee2\u5199\u7269\u3068\u3057\u3066\u8f38\u9001\u3055\u308c\u308b\u3068\u3001\u5b89\u5b9a\u7684\u306b\u7d30\u80de\u306b\u5c0e\u5165\u3055\u308c\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001RNA\u5206\u5b50\u306f\u305d\u308c\u3060\u3051\u3067\u306f\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u4f7f\u7528\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6226\u7565\u306e\u9078\u629e<\/h3>\n

\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u307e\u305f\u306f\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u3069\u3061\u3089\u3092\u9078\u629e\u3059\u308b\u304b\u306e\u6c7a\u5b9a\u306f\u3001\u5b9f\u65bd\u3057\u305f\u3044\u5b9f\u9a13\u306e\u671f\u9593\u304a\u3088\u3073\u6700\u7d42\u76ee\u6a19\u306b\u5fdc\u3058\u3066\u884c\u308f\u308c\u307e\u3059\u3002<\/strong>\u4e00\u904e\u7684\u306b\u5c0e\u5165\u3055\u308c\u305f\u7d30\u80de\u306f\u3001\u901a\u5e38\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5f8c24\uff5e96\u6642\u9593\u306e\u9593\u306b\u56de\u53ce\u3055\u308c\u3001\u591a\u304f\u306e\u5834\u5408\u3001\u907a\u4f1d\u5b50\u307e\u305f\u306f\u907a\u4f1d\u5b50\u7523\u7269\u306e\u77ed\u671f\u9593\u767a\u73fe\u306e\u52b9\u679c\u306b\u95a2\u3059\u308b\u7814\u7a76\u3001RNA\u5e72\u6e09\uff08RNAi\uff09\u5a92\u4ecb\u907a\u4f1d\u5b50\u30b5\u30a4\u30ec\u30f3\u30b7\u30f3\u30b0\u306e\u5b9f\u65bd\u3001\u3042\u308b\u3044\u306f\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8fc5\u901f\u306a\u5c0f\u898f\u6a21\u751f\u7523\u306b\u7528\u3044\u3089\u308c\u307e\u3059\u3002mRNA\u306f\u6838\u3078\u306e\u79fb\u884c\u304a\u3088\u3073\u8ee2\u5199\u30d7\u30ed\u30bb\u30b9\u3092\u5fc5\u8981\u3068\u305b\u305a\u306b\u30b5\u30a4\u30c8\u30be\u30eb\u3067\u767a\u73fe\u3055\u308c\u308b\u305f\u3081\u3001mRNA\u3092\u7528\u3044\u308b\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3067\u306f\u3055\u3089\u306b\u8fc5\u901f\u306b\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u4e00\u90e8\u306e\u30b7\u30b9\u30c6\u30e0\u3067\u306f\u3001\u5c0e\u5165\u3055\u308c\u305fmRNA\u3092\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5f8c\u6570\u5206\u4ee5\u5185\u306b\u767a\u73fe\u3055\u305b\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n

\u3053\u308c\u306b\u5bfe\u3057\u3066\u3001\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u9577\u671f\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u3084\u591a\u304f\u306e\u5b9f\u9a13\u3067\u7e70\u308a\u8fd4\u3057\u5c0e\u5165\u7d30\u80de\u304c\u5fc5\u8981\u3068\u3055\u308c\u308b\u5834\u5408\u306b\u3088\u308a\u6709\u7528\u3067\u3059\u3002<\/strong>DNA\u30d9\u30af\u30bf\u30fc\u306e\u67d3\u8272\u4f53\u3078\u306e\u7d44\u307f\u8fbc\u307f\u306f\u7a00\u306a\u30a4\u30d9\u30f3\u30c8\u3067\u3042\u308b\u305f\u3081\u3001\u7d30\u80de\u306e\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u9078\u629e\u7684\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3084\u30af\u30ed\u30fc\u30f3\u5358\u96e2\u3092\u5fc5\u8981\u3068\u3059\u308b\u3001\u3088\u308a\u591a\u304f\u306e\u6642\u9593\u3068\u52b4\u529b\u3092\u8981\u3059\u308b\u56f0\u96e3\u306a\u30d7\u30ed\u30bb\u30b9\u3067\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u901a\u5e38\u3001\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u5927\u898f\u6a21\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u751f\u7523\u3001\u3088\u308a\u9577\u671f\u7684\u306a\u85ac\u7406\u5b66\u7684\u7814\u7a76\u3001\u907a\u4f1d\u5b50\u7642\u6cd5\u3001\u3042\u308b\u3044\u306f\u9577\u671f\u7684\u306a\u907a\u4f1d\u5b50\u8abf\u7bc0\u6a5f\u69cb\u306b\u95a2\u3059\u308b\u7814\u7a76\u306b\u7528\u3044\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac\u304c\u8003\u6848\u3055\u308c\u3066\u304b\u3089\u3001\u54fa\u4e73\u985e\u7d30\u80de\u306e\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u6b63\u3057\u3044\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3084\u7ffb\u8a33\u5f8c\u4fee\u98fe\uff08\u7d30\u83cc\u7d30\u80de\u306b\u304a\u3051\u308b\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306b\u306f\u5b58\u5728\u3057\u306a\u3044\uff09\u3092\u53d7\u3051\u305f\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u751f\u7523\u306b\u7528\u3044\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u304c\u3001\u30df\u30ea\u30b0\u30e9\u30e0\u304b\u3089\u30b0\u30e9\u30e0\u5358\u4f4d\u306e\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u767a\u73fe\u3059\u308b\u80fd\u529b\u306f\u4e3b\u306b\u5b89\u5b9a\u7d30\u80de\u7cfb\u306e\u4f5c\u88fd\u306b\u4f9d\u5b58\u3057\u307e\u3059\u3002\u3055\u3089\u306b\u8fd1\u5e74\u306f\u3001\u6d6e\u904a\u57f9\u990a\u7528\u306b\u9069\u5fdc\u3055\u305b\u305fHEK293\u7d30\u80de\u304a\u3088\u3073CHO\u7d30\u80de\u306b\u3088\u308b\u5927\u91cf\u4e00\u904e\u6027\u767a\u73fe\u306b\u3088\u3063\u3066\u3001\u6642\u9593\u3068\u52b4\u529b\u3092\u8981\u3059\u308b\u5b89\u5b9a\u7d30\u80de\u7cfb\u958b\u767a\u306e\u30d7\u30ed\u30bb\u30b9\u3092\u5fc5\u8981\u3068\u305b\u305a\u306b\u591a\u91cf\u306e\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u3088\u308b\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306f\u3001\u76ee\u7684\u306e\u30d9\u30af\u30bf\u30fc\u3068\u6d6e\u904a\u57f9\u990a\u7cfb\u306eCHO\u7d30\u80de\u307e\u305f\u306fHEK293\u7d30\u80de\u304b\u3089\u958b\u59cb\u3057\u30663\uff5e7\u65e5\u3067\u3001\u9069\u5207\u306a\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3084\u7cd6\u9396\u4fee\u98fe\u3092\u53d7\u3051\u305f\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u3092mg\uff0fL\u5358\u4f4d\u3067\u751f\u7523\u3059\u308b\u3053\u3068\u3092\u53ef\u80fd\u3068\u3057\u307e\u3059\u3002<\/p>\n

\u54fa\u4e73\u985e\u7d30\u80de\u3067\u306e\u8fc5\u901f\u304b\u3064\u8d85\u9ad8\u53ce\u91cf\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u751f\u7523\u3092\u5b9f\u73fe\u3059\u308b\u305f\u3081\u306e\u4e00\u904e\u6027\u767a\u73fe\u6280\u8853\u306b\u304a\u3051\u308b\u5148\u9032\u306e\u30b7\u30b9\u30c6\u30e0\u306fGibco\u2122 Expi293\u2122 Expression System<\/a>\u3067\u3059\u3002<\/strong>\u3053\u308c\u306f\u3001Gibco\u2122 Expi293\u2122 Expression Medium<\/a>\u306b\u304a\u3051\u308bGibco\u2122 Expi293F\u2122\u7d30\u80de<\/a>\u306e\u9ad8\u5bc6\u5ea6\u57f9\u990a\u3068\u3001\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u30d9\u30fc\u30b9\u306eGibco\u2122 ExpiFectamine\u2122 293 \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac<\/a>\u3068\u6700\u9069\u5316\u3055\u308c\u305f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u30a8\u30f3\u30cf\u30f3\u30b5\u30fc\u3068\u306e\u7d44\u307f\u5408\u308f\u305b\u3092\u7528\u3044\u308b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u57fa\u3065\u304f\u3082\u306e\u3067\u3059\u3002\u5168\u3066\u306e\u69cb\u6210\u54c1\u304c\u9023\u643a\u3057\u3066\u50cd\u304f\u3053\u3068\u3067\u3001Gibco\u2122 FreeStyle\u2122 293 Expression System<\/a>\u306a\u3069\u306e\u5f93\u6765\u306e\u57f9\u990a\u30b7\u30b9\u30c6\u30e0\u306e2\uff5e10\u500d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u53ce\u91cf\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u3001IgG\u304a\u3088\u3073\u975eIgG\u30bf\u30f3\u30d1\u30af\u8cea\u30671 g\/L\u4ee5\u4e0a\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u304c\u9054\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/strong><\/p>\n

\u81e8\u5e8a\u7528\u751f\u7269\u88fd\u5264\u306e\u88fd\u9020\u306b\u306f\u3001\u30d0\u30c3\u30c1\u9593\u306e\u4e00\u8cab\u6027\u304a\u3088\u3073\u6975\u3081\u3066\u5927\u898f\u6a21\u306a\u30b9\u30b1\u30fc\u30eb\u306b\u304a\u3051\u308b\u4f4e\u30b3\u30b9\u30c8\u3067\u306e\u4f9b\u7d66\u304c\u5fc5\u8981\u3068\u3055\u308c\u308b\u305f\u3081\u3001\u5b89\u5b9a\u306b\u9ad8\u767a\u73fe\u3055\u308c\u308b\u5f62\u8cea\u8ee2\u63db\u4f53\u304c\u9ad8\u983b\u5ea6\u3067\u7528\u3044\u3089\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u591a\u304f\u306e\u5275\u85ac\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3044\u3066\u3001\u4e00\u9031\u9593\u4ee5\u5185\u306b\u69d8\u3005\u306a\u5019\u88dc\u5206\u5b50\u3092\u540c\u6642\u306b\u8a55\u4fa1\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306a\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3092\u7528\u3044\u3066\u8fc5\u901f\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u30b3\u30f3\u30b9\u30c8\u30e9\u30af\u30c8\u3092\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u3053\u3068\u306f\u6709\u76ca\u3067\u3059\u3002\u591a\u304f\u306e\u5834\u5408\u3001\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u9042\u884c\u3059\u308b\u306e\u306b3\u30f6\u6708\u4ee5\u4e0a\u8981\u3059\u308b\u3088\u308a\u8cc7\u6e90\u96c6\u7d04\u7684\u306a\u5b89\u5b9a\u7d30\u80de\u7cfb\u306e\u958b\u767a\u3068\u4e26\u884c\u3057\u3066\u7528\u3044\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
\u8868 \u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3068\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u9055\u3044<\/caption>\n
\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3<\/strong><\/td>\n\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3<\/strong><\/td>\n<\/tr>\n
\u5c0e\u5165\u3055\u308c\u305fDNA\u306f\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u307e\u308c\u305a\u3001\u6838\u306b\u7559\u307e\u308a\u307e\u3059\u3002<\/td>\n\u5c0e\u5165\u3055\u308c\u305fDNA\u306f\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u307e\u308c\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u5c0e\u5165\u3055\u308c\u305f\u907a\u4f1d\u7269\u8cea\u306f\u6b21\u4e16\u4ee3\u3078\u3068\u53d7\u3051\u7d99\u304c\u308c\u305a\u3001\u907a\u4f1d\u5b50\u5909\u7570\u306f\u6052\u4e45\u7684\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/td>\n\u5c0e\u5165\u3055\u308c\u305f\u907a\u4f1d\u7269\u8cea\u306f\u4e16\u4ee3\u304b\u3089\u4e16\u4ee3\u3078\u3068\u5b89\u5b9a\u7684\u306b\u53d7\u3051\u7d99\u304c\u308c\u3001\u907a\u4f1d\u5b50\u5909\u7570\u306f\u6052\u4e45\u7684\u3067\u3059\u3002<\/td>\n<\/tr>\n
\u9078\u629e\u7684\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u304c\u5fc5\u8981\u3042\u308a\u307e\u305b\u3093\u3002<\/td>\n\u5b89\u5b9a\u306a\u5f62\u8cea\u8ee2\u63db\u4f53\u3092\u5358\u96e2\u3059\u308b\u305f\u3081\u306e\u9078\u629e\u7684\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u304c\u5fc5\u8981\u3067\u3059\u3002<\/td>\n<\/tr>\n
\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u306fDNA\u30d9\u30af\u30bf\u30fc\u304a\u3088\u3073RNA\u306e\u4e21\u65b9\u3092\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/td>\n\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u306fDNA\u30d9\u30af\u30bf\u30fc\u306e\u307f\u3092\u4f7f\u7528\u3067\u304d\u3001RNA\u306f\u305d\u308c\u3060\u3051\u3067\u5b89\u5b9a\u7684\u306b\u7d30\u80de\u306b\u5c0e\u5165\u3067\u304d\u307e\u305b\u3093\u3002<\/td>\n<\/tr>\n
\u9ad8\u30b3\u30d4\u30fc\u6570\u3067\u907a\u4f1d\u7269\u8cea\u304c\u5c0e\u5165\u3055\u308c\u308b\u3053\u3068\u306f\u3001\u9ad8\u30ec\u30d9\u30eb\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002<\/td>\n\u5b89\u5b9a\u7684\u306b\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u307e\u308c\u308bDNA\u306f\u30b7\u30f3\u30b0\u30eb\u307e\u305f\u306f\u4f4e\u30b3\u30d4\u30fc\u6570\u3067\u3001\u4f4e\u30ec\u30d9\u30eb\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u7d30\u80de\u306f\u4e00\u822c\u7684\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5f8c24\uff5e96\u6642\u9593\u4ee5\u5185\u306b\u56de\u53ce\u3055\u308c\u307e\u3059\u3002<\/td>\n\u5b89\u5b9a\u7684\u306b\u5c0e\u5165\u3055\u308c\u305f\u30b3\u30ed\u30cb\u30fc\u3092\u5358\u96e2\u3059\u308b\u305f\u3081\u306b2\uff5e3\u9031\u9593\u306e\u9078\u5225\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u901a\u5e38\u3001\u8a98\u5c0e\u6027\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u6301\u3064\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u308b\u7814\u7a76\u306b\u306f\u9069\u3057\u307e\u305b\u3093\u3002<\/td>\n\u8a98\u5c0e\u6027\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u6301\u3064\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u308b\u7814\u7a76\u306b\u9069\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

RNA\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u305f\u3081\u306e\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u3068\u6700\u9069\u5316\u65b9\u6cd5<\/h2>\n

siRNA\u306a\u3069\u306eRNA\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3092\u884c\u3046\u969b\u306b\u4fbf\u5229\u306a\u60c5\u5831\u3092\u63b2\u8f09\u3057\u3066\u3044\u307e\u3059\u3002siRNA\u306e\u54c1\u8cea\u3084\u30dd\u30b8\u30c6\u30a3\u30d6\u3001\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306a\u3069\u57fa\u672c\u7684\u306a\u60c5\u5831\u3092\u78ba\u8a8d\u3057\u305f\u3044\u65b9\u306b\u30aa\u30b9\u30b9\u30e1\u3067\u3059\uff01<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/a><\/p>\n

Gibco\u7d30\u80de\u57f9\u990a\u57fa\u790e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h2>\n

\u7d30\u80de\u57f9\u990a\u306b\u95a2\u3059\u308b\u57fa\u790e\u60c5\u5831\u3092\u89e3\u8aac\u3057\u305f\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\uff08\u65e5\u672c\u8a9e\u7248\u3001\u7d04100\u30da\u30fc\u30b8\uff09\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u76ee\u7684\u304a\u3088\u3073\u305d\u306e\u624b\u7d9a\u304d\u4e0a\u3067\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u6a5f\u80fd\u89e3\u6790\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3084\u751f\u7523\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u6a5f\u80fd\u306e\u963b\u5bb3\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u5b9f\u9a13\u3067\u7d30\u80de\u3078\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u304c\u5fc5\u8981\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u306b\u306f\u3001\u7d30\u80de\u306b\u9069\u3057\u305f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306e\u9078\u629e\u304c\u5fc5\u8981\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u57fa\u790e\u77e5\u8b58\u3068\u3057\u3066\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u6982\u8981\u3068\u7a2e\u985e\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":988,"featured_media":2850,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[159],"tags":[67],"division":[],"class_list":{"0":"post-534","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-cell","8":"tag-transfection","9":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\n\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58 - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58\" \/>\n<meta property=\"og:description\" content=\"\u6a5f\u80fd\u89e3\u6790\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3084\u751f\u7523\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u6a5f\u80fd\u306e\u963b\u5bb3\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u5b9f\u9a13\u3067\u7d30\u80de\u3078\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u304c\u5fc5\u8981\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u306b\u306f\u3001\u7d30\u80de\u306b\u9069\u3057\u305f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306e\u9078\u629e\u304c\u5fc5\u8981\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u57fa\u790e\u77e5\u8b58\u3068\u3057\u3066\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u6982\u8981\u3068\u7a2e\u985e\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/\" \/>\n<meta property=\"og:site_name\" content=\"Learning at the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/ThermoFisherJapan\" \/>\n<meta property=\"article:published_time\" content=\"2016-12-19T00:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-02-12T06:19:50+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/basic-of-transfection-title.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"630\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"LATB Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:site\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"LATB Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"19\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/\"},\"author\":{\"name\":\"LATB Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\"},\"headline\":\"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58\",\"datePublished\":\"2016-12-19T00:00:00+00:00\",\"dateModified\":\"2025-02-12T06:19:50+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/\"},\"wordCount\":113,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/basic-of-transfection-title.jpg\",\"keywords\":[\"\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\"],\"articleSection\":[\"\u7d30\u80de\u57f9\u990a\u30fb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\"],\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/\",\"name\":\"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58 - Learning at the Bench\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/basic-of-transfection-title.jpg\",\"datePublished\":\"2016-12-19T00:00:00+00:00\",\"dateModified\":\"2025-02-12T06:19:50+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/#breadcrumb\"},\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/basic-of-transfection-title.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/basic-of-transfection-title.jpg\",\"width\":1200,\"height\":630,\"caption\":\"basic-of-transfection-title\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/basic-of-transfection\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\",\"name\":\"Learning at the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"ja\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\",\"name\":\"LATB Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"caption\":\"LATB Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/latbstaff\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58 - Learning at the Bench","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/","og_locale":"ja_JP","og_type":"article","og_title":"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58","og_description":"\u6a5f\u80fd\u89e3\u6790\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3084\u751f\u7523\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u6a5f\u80fd\u306e\u963b\u5bb3\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u5b9f\u9a13\u3067\u7d30\u80de\u3078\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u304c\u5fc5\u8981\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u306b\u306f\u3001\u7d30\u80de\u306b\u9069\u3057\u305f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306e\u9078\u629e\u304c\u5fc5\u8981\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u57fa\u790e\u77e5\u8b58\u3068\u3057\u3066\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u6982\u8981\u3068\u7a2e\u985e\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002","og_url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/","og_site_name":"Learning at the Bench","article_publisher":"https:\/\/www.facebook.com\/ThermoFisherJapan","article_published_time":"2016-12-19T00:00:00+00:00","article_modified_time":"2025-02-12T06:19:50+00:00","og_image":[{"width":1200,"height":630,"url":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/basic-of-transfection-title.jpg","type":"image\/jpeg"}],"author":"LATB Staff","twitter_card":"summary_large_image","twitter_creator":"@ThermoFisherJP","twitter_site":"@ThermoFisherJP","twitter_misc":{"\u57f7\u7b46\u8005":"LATB Staff","\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593":"19\u5206"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/"},"author":{"name":"LATB Staff","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d"},"headline":"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58","datePublished":"2016-12-19T00:00:00+00:00","dateModified":"2025-02-12T06:19:50+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/"},"wordCount":113,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/basic-of-transfection-title.jpg","keywords":["\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3"],"articleSection":["\u7d30\u80de\u57f9\u990a\u30fb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0"],"inLanguage":"ja","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/","url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/","name":"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58 - Learning at the Bench","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/basic-of-transfection-title.jpg","datePublished":"2016-12-19T00:00:00+00:00","dateModified":"2025-02-12T06:19:50+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d"},"breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/#breadcrumb"},"inLanguage":"ja","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/"]}]},{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/basic-of-transfection-title.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/basic-of-transfection-title.jpg","width":1200,"height":630,"caption":"basic-of-transfection-title"},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/"},{"@type":"ListItem","position":2,"name":"\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/","name":"Learning at the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"ja"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d","name":"LATB Staff","image":{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","caption":"LATB Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/latbstaff\/"}]}},"taxonomy_info":{"category":[{"value":159,"label":"\u7d30\u80de\u57f9\u990a\u30fb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0"}],"post_tag":[{"value":67,"label":"\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/basic-of-transfection-title-1024x538.jpg",760,399,true],"author_info":{"display_name":"LATB Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/latbstaff\/"},"comment_info":0,"category_info":[{"term_id":159,"name":"\u7d30\u80de\u57f9\u990a\u30fb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0","slug":"cell","term_group":0,"term_taxonomy_id":159,"taxonomy":"category","description":"","parent":0,"count":103,"filter":"raw","meta":[],"cat_ID":159,"category_count":103,"category_description":"","cat_name":"\u7d30\u80de\u57f9\u990a\u30fb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0","category_nicename":"cell","category_parent":0}],"tag_info":[{"term_id":67,"name":"\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3","slug":"transfection","term_group":0,"term_taxonomy_id":67,"taxonomy":"post_tag","description":"","parent":0,"count":17,"filter":"raw","meta":[]}],"jetpack-related-posts":[{"id":23864,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-expression-cloning-bid-ts-mbr-25070\/","url_meta":{"origin":534,"position":0},"title":"\u521d\u5fc3\u8005\u3067\u3082\u5b89\u5fc3\uff01\u7c21\u5358\u306b\u3067\u304d\u308b\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u4f5c\u308a\u65b9\uff1a\u4eba\u5de5\u907a\u4f1d\u5b50\u5408\u6210\u30b5\u30fc\u30d3\u30b9\u306e\u5229\u7528\u6cd5","author":"LatB Staff","date":"2025\u5e7411\u670813\u65e5","format":false,"excerpt":"\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u5b9f\u9a13\u306b\u306f\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u304c\u6b20\u304b\u305b\u307e\u305b\u3093\u304c\u3001\u4e00\u304b\u3089\u4f5c\u308b\u3068\u306a\u308b\u3068\u3001\u304b\u306a\u308a\u306e\u624b\u9593\u3068\u7d4c\u9a13\u304c\u5fc5\u8981\u3067\u3059\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/protein-expression-cloning-bid-ts-mbr-25070_eyecatch.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/protein-expression-cloning-bid-ts-mbr-25070_eyecatch.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/protein-expression-cloning-bid-ts-mbr-25070_eyecatch.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/protein-expression-cloning-bid-ts-mbr-25070_eyecatch.png?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":24606,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/transfection-antibody-bid-ts-mbr-26004\/","url_meta":{"origin":534,"position":1},"title":"\u7d30\u80de\u5185\u30bf\u30f3\u30d1\u30af\u8cea\u9664\u53bb\u30b7\u30b9\u30c6\u30e0\uff08Trim-Away\u6cd5\uff09\u306e\u7d39\u4ecb\uff5e\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6a5f\u5668\u3092\u4f7f\u7528\u3057\u3066\uff5e","author":"LatB Staff","date":"2026\u5e742\u67084\u65e5","format":false,"excerpt":"2018\u5e74\u306e\u6587\u732e(1)\u3067\u7d30\u80de\u5185\u306e\u5185\u5728\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u76f4\u63a5\u5206\u89e3\u3059\u308b\u3001Trim-Away\u6cd5\u304c\u7d39\u4ecb\u3055\u308c\u307e\u3057\u305f\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/01\/transfection-antibody-bid-ts-mbr-26004_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/01\/transfection-antibody-bid-ts-mbr-26004_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/01\/transfection-antibody-bid-ts-mbr-26004_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/01\/transfection-antibody-bid-ts-mbr-26004_eyecatch.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":23239,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ngs-gene-expression-csd-ts-bfx-25060\/","url_meta":{"origin":534,"position":2},"title":"\u98df\u54c1\u958b\u767a\u306b\u304a\u3051\u308b\u83cc\u53e2\u89e3\u6790\u3068\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306e\u6d3b\u7528","author":"LatB Staff","date":"2025\u5e7410\u67088\u65e5","format":false,"excerpt":"\u907a\u4f1d\u5b50\u95a2\u9023\u306e\u89e3\u6790\u6280\u8853\u304c\u7814\u7a76\u306e\u307f\u306a\u3089\u305a\u7523\u696d\u5206\u91ce\u3067\u6d3b\u7528\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u3001\u5e74\u6708\u304c\u7d4c\u904e\u3059\u308b\u306b\u3064\u308c\u3066\u5fdc\u7528\u7bc4\u56f2\u3082\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/09\/ngs-gene-expression-gsd-ts-tr-25060_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/09\/ngs-gene-expression-gsd-ts-tr-25060_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/09\/ngs-gene-expression-gsd-ts-tr-25060_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":24152,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell-culture-protein-expression-bid-ts-mbr-25074\/","url_meta":{"origin":534,"position":3},"title":"\u30bf\u30f3\u30d1\u30af\u7523\u751f\u3084\u30a6\u30a4\u30eb\u30b9\u7523\u751f\u3092\u76ee\u7684\u3068\u3057\u305f\u9ad8\u5bc6\u5ea6\u7d30\u80de\u57f9\u990a\u30b7\u30b9\u30c6\u30e0\u5c0e\u5165\u306e\u7b2c\u4e00\u6b69\uff1a\u7d30\u80de\u682a\u306e\u7d99\u4ee3\u3068\u51cd\u7d50\u4fdd\u5b58\u7de8","author":"LatB Staff","date":"2025\u5e7412\u67083\u65e5","format":false,"excerpt":"\u9ad8\u5bc6\u5ea6\u7d30\u80de\u57f9\u990a\u30b7\u30b9\u30c6\u30e0\u3067\u306f\u3001\u5c02\u7528\u306e\u6d6e\u904a\u7d30\u80de\u682a\u3092\u7528\u3044\u3066\u6c7a\u3081\u3089\u308c\u305f\u624b\u9806\u3067\u57f9\u990a\u3057\u307e\u3059\u3002\u5b89\u5b9a\u7684\u306b\u904b\u7528\u3059\u308b\u305f\u3081\u2026","rel":"","context":"\u7d30\u80de\u57f9\u990a\u30fb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0","block_context":{"text":"\u7d30\u80de\u57f9\u990a\u30fb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/cell\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/cell-culture-protein-expression-bid-ts-mbr-25074_eycatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/cell-culture-protein-expression-bid-ts-mbr-25074_eycatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/cell-culture-protein-expression-bid-ts-mbr-25074_eycatch.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/cell-culture-protein-expression-bid-ts-mbr-25074_eycatch.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":20124,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-gene-therapy-ts-r-25015\/","url_meta":{"origin":534,"position":4},"title":"\u3010\u7b2c1\u56de\u3011Exosome\uff08\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\uff0f\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09\u3068\u306f\uff1f","author":"LatB Staff","date":"2026\u5e744\u670815\u65e5","format":false,"excerpt":"Exosome\uff08\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\uff0f\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09\u306e\u7814\u7a76\u304c\u6ce8\u76ee\u3092\u96c6\u3081\u3066\u3044\u307e\u3059\u3002\u8208\u5473\u306f\u3042\u3063\u3066\u3082\u3001\u3069\u306e\u3088\u3046\u306b\u7814\u2026","rel":"","context":"\u305d\u306e\u4ed6","block_context":{"text":"\u305d\u306e\u4ed6","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/else\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":24187,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ngs-gene-expression-csd-ts-ion-25073\/","url_meta":{"origin":534,"position":5},"title":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u5fae\u91cf\u30fb\u5206\u89e3RNA\u306b\u3088\u308b\u767a\u73fe\u89e3\u6790\u306e\u6700\u9069\u89e3\u306f\uff1fRNA-Seq\u3068Ion AmpliSeq Transcriptome\u3092\u6bd4\u3079\u3066\u307f\u305f","author":"LatB Staff","date":"2025\u5e7411\u670828\u65e5","format":false,"excerpt":"\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u306e\u767a\u73fe\u89e3\u6790\u306b\u53d6\u308a\u7d44\u3080\u969b\u3001RNA\u30b5\u30f3\u30d7\u30eb\u304c\u300c\u5c11\u306a\u3059\u304e\u308b\u300d\u300c\u5206\u89e3\u3057\u3066\u3044\u308b\u300d\u3068\u3044\u3046\u72b6\u6cc1\u306f\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"amp_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/basic-of-transfection-title.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/534","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/users\/988"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/comments?post=534"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/534\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media\/2850"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media?parent=534"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/categories?post=534"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/tags?post=534"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/division?post=534"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}