{"id":536,"date":"2017-01-04T00:00:00","date_gmt":"2017-01-04T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-delivery-technologies\/"},"modified":"2025-02-26T17:07:40","modified_gmt":"2025-02-26T08:07:40","slug":"gene-delivery-technologies","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-delivery-technologies\/","title":{"rendered":"\u5fb9\u5e95\u7db2\u7f85\uff01\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u304a\u3051\u308b\u5316\u5b66\u7684\u624b\u6cd5\u3001\u751f\u7269\u5b66\u7684\u624b\u6cd5\u3001\u7269\u7406\u7684\u624b\u6cd5\u307e\u3068\u3081"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>\u7d30\u80de\u819c\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u57cb\u3081\u8fbc\u307e\u308c\u305f\u30ea\u30f3\u8102\u8cea\u4e8c\u91cd\u819c\u304b\u3089\u69cb\u6210\u3055\u308c\u3001\u6b63\u5473\u306e\u8ca0\u96fb\u8377\u3092\u6301\u3061\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u7d30\u80de\u819c\u306fDNA\u3084RNA\u306e\u30ea\u30f3\u9178\u9aa8\u683c\u306e\u3088\u3046\u306a\u540c\u3058\u304f\u8ca0\u306b\u8377\u96fb\u3057\u3066\u3044\u308b\u5de8\u5927\u5206\u5b50\u3092\u901a\u904e\u3055\u305b\u306a\u3044\u305f\u3081\u306e\u30d0\u30ea\u30a2\u3068\u3057\u3066\u5b58\u5728\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u3001\u6838\u9178\u306e\u7d30\u80de\u819c\u901a\u904e\u3092\u53ef\u80fd\u3068\u3059\u308b\u305f\u3081\u306b\u3001\u5316\u5b66\u7269\u8cea\u3084\u62c5\u4f53\u5206\u5b50\u3092\u7528\u3044\u3066\u6838\u9178\u3092\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u3057\u3066\u4e2d\u548c\u3059\u308b\u5316\u5b66\u7684\u624b\u6cd5\u304b\u3089\u3001DNA\u3092\u76f4\u63a5\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u305f\u3081\u306b\u819c\u4e0a\u306b\u4e00\u6642\u7684\u306b\u5b54\u3092\u958b\u3051\u308b\u7269\u7406\u7684\u624b\u6cd5\u307e\u3067\u3001\u5404\u7a2e\u69d8\u3005\u306a\u30a2\u30d7\u30ed\u30fc\u30c1\u3092\u7528\u3044\u305f\u6570\u591a\u304f\u306e\u6280\u8853\u304c\u958b\u767a\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n<p><strong>\u73fe\u5728\u4f7f\u7528\u53ef\u80fd\u306a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6280\u8853\u306f\u3001\u4ee5\u4e0b\u306e3\u7a2e\u985e\u306b\u5927\u304d\u304f\u5206\u985e\u3055\u308c\u307e\u3059\u3002\u5316\u5b66\u7684\u624b\u6cd5<\/strong>\u306f\u3001\u62c5\u4f53\u5206\u5b50\u3092\u7528\u3044\u3066\u8ca0\u306b\u8377\u96fb\u3057\u305f\u6838\u9178\u3092\u4e2d\u548c\u3059\u308b\u304b\u6b63\u96fb\u8377\u3092\u4ed8\u4e0e\u3059\u308b\u624b\u6cd5\u3001<strong>\u751f\u7269\u5b66\u7684\u624b\u6cd5<\/strong>\u306f\u3001\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u3092\u4ecb\u3057\u3066\u975e\u30a6\u30a4\u30eb\u30b9\u907a\u4f1d\u5b50\u3092\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u624b\u6cd5\uff08\u5225\u540d\uff1a \u5f62\u8cea\u5c0e\u5165\uff09\u3001\u305d\u3057\u3066<strong>\u7269\u7406\u7684\u624b\u6cd5<\/strong>\u306f\u3001\u7d30\u80de\u306e\u7d30\u80de\u8cea\u307e\u305f\u306f\u6838\u306b\u6838\u9178\u3092\u76f4\u63a5\u9001\u9054\u3059\u308b\u624b\u6cd5\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u3053\u308c\u3089\u306e\u624b\u6cd5\u306e\u4e2d\u3067\u5168\u3066\u306e\u7d30\u80de\u304a\u3088\u3073\u5168\u3066\u306e\u5b9f\u9a13\u306b\u9069\u5408\u3059\u308b\u3082\u306e\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001\u7d30\u80de\u30bf\u30a4\u30d7\u304a\u3088\u3073\u5b9f\u9a13\u30cb\u30fc\u30ba\u306b\u5fdc\u3058\u3066\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u9ad8\u304f\u3001\u7d30\u80de\u6bd2\u6027\u304c\u4f4e\u304f\u3001\u6b63\u5e38\u306a\u751f\u7406\u6a5f\u80fd\u3078\u306e\u5f71\u97ff\u304c\u6700\u5c0f\u9650\u3068\u306a\u308b\u3001\u7c21\u4fbf\u3067\u518d\u73fe\u6027\u306e\u9ad8\u3044\u7406\u60f3\u7684\u306a\u30a2\u30d7\u30ed\u30fc\u30c1\u3092\u9078\u629e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\uff08Kim and Eberwine, 2010\uff09\u3002<\/p>\n<p><strong>\u4eca\u56de\u306f\u907a\u4f1d\u5b50\u5c0e\u5165\u65b9\u6cd5\u306b\u304a\u3051\u308b\u5316\u5b66\u7684\u624b\u6cd5\u3001\u751f\u7269\u5b66\u7684\u624b\u6cd5\u3001\u7269\u7406\u7684\u624b\u6cd5\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u306e\u3067\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/strong><\/p>\n<h2>\u5316\u5b66\u7684\u907a\u4f1d\u5b50\u5c0e\u5165\u6cd5<\/h2>\n<h3>\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u5a92\u4ecb\u6027\u5c0e\u5165<\/h3>\n<p><strong>\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u5916\u6765\u907a\u4f1d\u7269\u8cea\u3092\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u305f\u3081\u306e\u6700\u3082\u4e00\u822c\u7684\u306a\u624b\u6cd5\u306e1\u3064\u3067\u3059\u3002<\/strong>\u7b2c\u4e00\u4e16\u4ee3\u306e\u8102\u8cea\u30d9\u30fc\u30b9\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac\u3067\u306f\u3001\u6838\u9178\u3092\u5c01\u5165\u3057\u305f\u5f8c\u3001\u7d30\u80de\u819c\u3092\u6eb6\u89e3\u3057\u3001\u305d\u306e\u30ab\u30fc\u30b4\u3092\u5185\u90e8\u306b\u84c4\u7a4d\u3059\u308b\u4eba\u5de5\u30ea\u30dd\u30bd\u30fc\u30e0\u304c\u5229\u7528\u3055\u308c\u307e\u3057\u305f\u304c\uff08Fraley et al., 1980\uff09\u3001\u65b0\u3057\u3044\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u30d9\u30fc\u30b9\u306e\u8a66\u85ac\u3067\u306f\u3001\u8ca0\u306b\u8377\u96fb\u3057\u305f\u6838\u9178\u3068\u6b63\u306b\u8377\u96fb\u3057\u305f\u5408\u6210\u8102\u8cea\u8a66\u85ac\u306e\u982d\u90e8\u57fa\u3068\u306e\u9593\u306e\u9759\u96fb\u76f8\u4e92\u4f5c\u7528\u306b\u3088\u3063\u3066\u81ea\u767a\u7684\u306a\u7e2e\u5408\u304c\u751f\u3058\u3001\u6838\u9178\u2015\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u8a66\u85ac\u8907\u5408\u4f53\u304c\u5f62\u6210\u3055\u308c\u307e\u3059\u3002\u8907\u5408\u4f53\u306f\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3092\u4ecb\u3057\u3066\u7d30\u80de\u306b\u53d6\u308a\u8fbc\u307e\u308c\u3001\u7d30\u80de\u8cea\u306b\u653e\u51fa\u3055\u308c\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u4e00\u65e6\u8907\u5408\u4f53\u304c\u7d30\u80de\u5185\u306b\u5165\u308b\u3068\u3001\u5c0e\u5165\u3055\u308c\u305fDNA\u306f\u6838\u306b\u79fb\u884c\u3057\u3001\u672a\u77e5\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u3088\u3063\u3066\u767a\u73fe\u3055\u308c\u307e\u3059\u304c\u3001RNA\u3084\u30a2\u30f3\u30c1\u30bb\u30f3\u30b9\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306f\u79fb\u884c\u30b9\u30c6\u30c3\u30d7\u3092\u30b9\u30ad\u30c3\u30d7\u3057\u3001\u7d30\u80de\u8cea\u5185\u306b\u7559\u307e\u308a\u307e\u3059\u3002<\/p>\n<div id=\"attachment_1585\" style=\"width: 851px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig1.png\" rel=\"attachment wp-att-1585\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1585\" class=\"size-full wp-image-1585\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig1.png\" alt=\"gene-delivery-technologies-fig1\" width=\"841\" height=\"747\" \/><\/a><p id=\"caption-attachment-1585\" class=\"wp-caption-text\">\u56f31 \u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u5a92\u4ecb\u6027\u5c0e\u5165\u306e\u30e1\u30ab\u30cb\u30ba\u30e0<\/p><\/div>\n<p><strong>\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u5229\u70b9<\/strong>\u306f\u3001\u5e45\u5e83\u3044\u7d30\u80de\u7cfb\u306e\u9ad8\u52b9\u7387\u306a\u5c0e\u5165\u304c\u53ef\u80fd\u3067\u3001\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306b\u9069\u5408\u3057\u3001\u5168\u30b5\u30a4\u30ba\u306eDNA\u306b\u52a0\u3048\u3066RNA\u304a\u3088\u3073\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5c0e\u5165\u3082\u53ef\u80fd\u306a\u70b9\u3067\u3059\u3002\u3055\u3089\u306b\u3001\u3053\u306e\u624b\u6cd5\u306f\u5b89\u5b9a\u304a\u3088\u3073\u4e00\u904e\u6027\u767a\u73fe\u306e\u4e21\u65b9\u306b\u9069\u5408\u3057\u3001\u4ed6\u306e\u5316\u5b66\u7684\u624b\u6cd5\u3068\u306f\u7570\u306a\u308a\u3001DNA\u304a\u3088\u3073RNA\u306e\u52d5\u7269\u304a\u3088\u3073\u30d2\u30c8\u3078\u306ein vivo\u5c0e\u5165\u306b\u3082\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<strong>\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u4e3b\u306a\u6b20\u70b9<\/strong>\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u7d30\u80de\u30bf\u30a4\u30d7\u3068\u57f9\u990a\u6761\u4ef6\u306b\u4f9d\u5b58\u3059\u308b\u305f\u3081\u3001\u305d\u308c\u305e\u308c\u306e\u7d30\u80de\u30bf\u30a4\u30d7\u3068\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac\u306b\u3064\u3044\u3066\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6761\u4ef6\u3092\u6700\u9069\u5316\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u70b9\u3067\u3059\u3002<\/p>\n<p>\u5f0a\u793e\u306f\u3001DNA\u3001RNA\u3001siRNA\u3001\u307e\u305f\u306f\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3092\u5e45\u5e83\u3044\u30bf\u30a4\u30d7\u306e\u7d30\u80de\u306b\u52b9\u7387\u7684\u306b\u5c0e\u5165\u3059\u308b\u305f\u3081\u306e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-expression-and-analysis\/transfection-selection\/lipofectamine-3000.html?CID=bid_clb_tfx_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cc13_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Invitrogen\u2122 Lipofectamine\u2122 3000 Transfection Reagent<\/a>\u3092\u306f\u3058\u3081\u3068\u3059\u308b\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-expression-and-analysis\/transfection-selection\/lipofectamine-3000.html?CID=bid_clb_tfx_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cc13_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Lipofectamine 3000\u8a66\u85ac<\/a>\u306f\u3001\u6700\u3082\u5148\u9032\u306e\u8102\u8cea\u30ca\u30ce\u7c92\u5b50\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3092\u5229\u7528\u3057\u3066\u304a\u308a\u3001\u69d8\u3005\u306a\u5c0e\u5165\u56f0\u96e3\u306a\u7d30\u80de\u306b\u304a\u3051\u308b\u512a\u308c\u305f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3068\u518d\u73fe\u6027\u306e\u9ad8\u3044\u7d50\u679c\u3092\u5b9f\u73fe\u3057\u3001\u7d30\u80de\u751f\u5b58\u7387\u3082\u6539\u5584\u3057\u307e\u3059\u3002<\/p>\n<div id=\"attachment_1586\" style=\"width: 1127px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig2.png\" rel=\"attachment wp-att-1586\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1586\" class=\"size-full wp-image-1586\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig2.png\" alt=\"gene-delivery-technologies-fig2\" width=\"1117\" height=\"417\" \/><\/a><p id=\"caption-attachment-1586\" class=\"wp-caption-text\">\u56f32 \u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/p><\/div>\n<h3>\u30ea\u30f3\u9178\u30ab\u30eb\u30b7\u30a6\u30e0\u5171\u6c88\u6bbf\u6cd5<\/h3>\n<p><strong>\u30ea\u30f3\u9178\u30ab\u30eb\u30b7\u30a6\u30e0\u5171\u6c88\u6bbf\u6cd5\u306f\u3001\u6210\u5206\u304c\u5165\u624b\u3057\u3084\u3059\u304f\u3001\u5b89\u4fa1\u3067\u3042\u308b\u305f\u3081\u30011970\u5e74\u4ee3\u521d\u671f\u306b\u5c0e\u5165\u3055\u308c\u3066\u4ee5\u6765\uff08Graham and van der Eb, 1973\uff09\u3001\u3088\u304f\u7528\u3044\u3089\u308c\u3066\u3044\u308b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3067\u3059\u3002<\/strong>\u3055\u3089\u306b\u3001\u3053\u306e\u624b\u6cd5\u306f\u30de\u30b9\u30bf\u30fc\u3057\u3084\u3059\u304f\u3001\u591a\u304f\u306e\u30bf\u30a4\u30d7\u306e\u57f9\u990a\u7d30\u80de\u306b\u52b9\u679c\u7684\u3067\u3042\u308a\u3001\u69d8\u3005\u306a\u57f9\u990a\u7d30\u80de\u30bf\u30a4\u30d7\u306e\u4e00\u904e\u6027\u304a\u3088\u3073\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u4f7f\u7528\u53ef\u80fd\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u30ea\u30f3\u9178\u30ab\u30eb\u30b7\u30a6\u30e0\u5171\u6c88\u6bbf\u6cd5\u306f\u3001pH\u3001\u6e29\u5ea6\u3001\u304a\u3088\u3073\u30d0\u30c3\u30d5\u30a1\u30fc\u5869\u6fc3\u5ea6\u306e\u5fae\u5c0f\u306a\u5909\u5316\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u304c\u9ad8\u3044\u305f\u3081\u5909\u52d5\u3057\u3084\u3059\u3044\u50be\u5411\u304c\u3042\u308a\u3001\u307e\u305f\u591a\u304f\u306e\u30bf\u30a4\u30d7\u306e\u7d30\u80de\u306e\u57f9\u990a\u3001\u7279\u306b\u521d\u4ee3\u7d30\u80de\u306e\u57f9\u990a\u306b\u304a\u3044\u3066\u6bd2\u6027\u3092\u6301\u3064\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u52a0\u3048\u3066\u3001\u5168\u3066\u306e\u52d5\u7269\u306b\u5bfe\u3059\u308bin vivo\u6838\u9178\u5c0e\u5165\u304c\u9069\u3055\u305a\u3001\u8102\u8cea\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306a\u3069\u306e\u4ed6\u306e\u5316\u5b66\u7684\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306b\u6bd4\u3079\u3066\u6bd4\u8f03\u7684\u4f4e\u3044\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n<p><strong>\u30ea\u30f3\u9178\u30ab\u30eb\u30b7\u30a6\u30e0\u5171\u6c88\u6bbf\u6cd5\u306e\u539f\u7406<\/strong>\u306f\u3001\u30ea\u30f3\u9178\u7de9\u885d\u751f\u7406\u98df\u5869\u6c34\u4e2d\u3067DNA\u3092\u5869\u5316\u30ab\u30eb\u30b7\u30a6\u30e0\u6eb6\u6db2\u3068\u6df7\u5408\u3059\u308b\u3068\u3001\u30ea\u30f3\u9178\u30ab\u30eb\u30b7\u30a6\u30e0-DNA\u5171\u6c88\u6bbf\u7269\u304c\u751f\u3058\u3001\u3053\u306e\u6c88\u6bbf\u306e\u61f8\u6fc1\u6db2\u3092\u57f9\u990a\u7d30\u80de\u306e\u4e0a\u306b\u4e57\u305b\u308b\u3053\u3068\u3067DNA\u304c\u53d6\u308a\u8fbc\u307e\u308c\u308b\u3068\u3044\u3046\u3082\u306e\u3067\u3059\u3002\u30ea\u30f3\u9178\u30ab\u30eb\u30b7\u30a6\u30e0\u306f\u5171\u6c88\u6bbf\u7269\u4e2d\u306b\u6fc3\u7e2e\u3055\u308c\u305fDNA\u306e\u7d30\u80de\u8868\u9762\u3078\u306e\u7d50\u5408\u3092\u4fc3\u9032\u3057\u3001DNA\u306f\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u306b\u3088\u308a\u7d30\u80de\u306b\u4fb5\u5165\u3057\u307e\u3059\u3002DNA\u0336\u5869\u5316\u30ab\u30eb\u30b7\u30a6\u30e0\u6eb6\u6db2\u3092\u30ea\u30f3\u9178\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6dfb\u52a0\u3057\u3066\u3044\u308b\u9593\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u901a\u6c17\u3092\u4f9b\u7d66\u3059\u308b\u3053\u3068\u306f\u3001\u3067\u304d\u308b\u3060\u3051\u5fae\u7d30\u306a\u6c88\u6bbf\u3092\u78ba\u5b9f\u306b\u5f62\u6210\u3055\u305b\u308b\u305f\u3081\u306b\u91cd\u8981\u3067\u3059\u3002\u306a\u305c\u306a\u3089\u3001DNA\u306f\u51dd\u96c6\u3059\u308b\u3068\u7d30\u80de\u306b\u4ed8\u7740\u3059\u308b\u3053\u3068\u3082\u52b9\u7387\u826f\u304f\u7d30\u80de\u306b\u4fb5\u5165\u3059\u308b\u3053\u3068\u3082\u56f0\u96e3\u3068\u306a\u308b\u304b\u3089\u3067\u3059\u3002<\/p>\n<div id=\"attachment_1587\" style=\"width: 849px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig3.png\" rel=\"attachment wp-att-1587\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1587\" class=\"size-full wp-image-1587\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig3.png\" alt=\"gene-delivery-technologies-fig3\" width=\"839\" height=\"666\" \/><\/a><p id=\"caption-attachment-1587\" class=\"wp-caption-text\">\u56f33 \u30ea\u30f3\u9178\u30ab\u30eb\u30b7\u30a6\u30e0\u5171\u6c88\u6bbf\u6cd5\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/p><\/div>\n<h3>DEAE-\u30c7\u30ad\u30b9\u30c8\u30e9\u30f3\u6cd5\u306b\u3088\u308b\u5c0e\u5165<\/h3>\n<p><strong>\u30b8\u30a8\u30c1\u30eb\u30a2\u30df\u30ce\u30a8\u30c1\u30eb\uff08DEAE\uff09\u2015 \u30c7\u30ad\u30b9\u30c8\u30e9\u30f3\u306f\u3001\u7cd6\u8cea\u30dd\u30ea\u30de\u30fc\uff08\u30c7\u30ad\u30b9\u30c8\u30e9\u30f3\uff09\u306e\u30dd\u30ea\u30ab\u30c1\u30aa\u30f3\u6027\u8a98\u5c0e\u4f53\u3067\u3001\u6838\u9178\u306e\u57f9\u990a\u54fa\u4e73\u985e\u7d30\u80de\u3078\u306e\u5c0e\u5165\u306b\u7528\u3044\u3089\u308c\u305f\u6700\u521d\u306e\u5316\u5b66\u8a66\u85ac\u306e\u4e00\u3064\u3067\u3059<\/strong>\uff08Vaheri and Pagano, 1965\uff09\u3002\u30ab\u30c1\u30aa\u30f3\u6027DEAE \u30c7\u30ad\u30b9\u30c8\u30e9\u30f3\u5206\u5b50\u306f\u3001\u8ca0\u306b\u8377\u96fb\u3057\u305f\u6838\u9178\u9aa8\u683c\u306b\u5f37\u56fa\u306b\u7d50\u5408\u3057\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u751f\u3058\u305f\u6838\u9178 DEAE \u30c7\u30ad\u30b9\u30c8\u30e9\u30f3\u8907\u5408\u4f53\u306f\u6b63\u5473\u306e\u6b63\u96fb\u8377\u3092\u6301\u3064\u305f\u3081\u3001\u7d30\u80de\u819c\u306b\u4ed8\u7740\u3057\u3001\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3042\u308b\u3044\u306fDMSO\u307e\u305f\u306f\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u306b\u3088\u3063\u3066\u8a98\u5c0e\u3055\u308c\u308b\u6d78\u900f\u5727\u30b7\u30e7\u30c3\u30af\u306b\u3088\u308a\u7d30\u80de\u8cea\u306b\u4fb5\u5165\u3057\u307e\u3059\u3002<\/p>\n<p><strong>DEAE \u30c7\u30ad\u30b9\u30c8\u30e9\u30f3\u6cd5\u306e\u5229\u70b9<\/strong>\u306f\u3001\u6bd4\u8f03\u7684\u30b7\u30f3\u30d7\u30eb\u3067\u3001\u518d\u73fe\u6027\u304c\u9ad8\u304f\u3001\u4f4e\u30b3\u30b9\u30c8\u3067\u3042\u308b\u70b9\u3067\u3059\u3002\u6b20\u70b9\u306f\u3001\u7d30\u80de\u6bd2\u6027\u3001\u5e45\u5e83\u3044\u7d30\u80de\u30bf\u30a4\u30d7\u306b\u304a\u3051\u308b\u4f4e\u3044\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\uff08\u5178\u578b\u7684\u306b\u306f\u521d\u4ee3\u7d30\u80de\u306710%\u4ee5\u4e0b\uff09\u3001\u306a\u3089\u3073\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u51e6\u7406\u4e2d\u306b\u8840\u6e05\u542b\u6709\u7387\u306e\u4f4e\u3044\u57f9\u5730\u3092\u4f7f\u7528\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u70b9\u3067\u3059\u3002\u307e\u305f\u3001\u3053\u306e\u624b\u6cd5\u306e\u4f7f\u7528\u306f\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u9650\u5b9a\u3055\u308c\u3001\u5b89\u5b9a\u7d30\u80de\u7cfb\u306e\u4f5c\u88fd\u306b\u306f\u9069\u3057\u307e\u305b\u3093\u3002<\/p>\n<div id=\"attachment_1588\" style=\"width: 845px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig4.png\" rel=\"attachment wp-att-1588\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1588\" class=\"size-full wp-image-1588\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig4.png\" alt=\"gene-delivery-technologies-fig4\" width=\"835\" height=\"907\" \/><\/a><p id=\"caption-attachment-1588\" class=\"wp-caption-text\">\u56f34 DEAE-\u30c7\u30ad\u30b9\u30c8\u30e9\u30f3\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/p><\/div>\n<h3>\u4ed6\u306e\u30ab\u30c1\u30aa\u30f3\u6027\u30dd\u30ea\u30de\u30fc\u306b\u3088\u308b\u5c0e\u5165<\/h3>\n<p><strong>\u907a\u4f1d\u5b50\u5c0e\u5165\u306b\u4f7f\u7528\u3055\u308c\u308b\u4ed6\u306e\u30ab\u30c1\u30aa\u30f3\u6027\u30dd\u30ea\u30de\u30fc<\/strong>\u3068\u3057\u3066\u3001\u30ab\u30c1\u30aa\u30f3\u6027\u30da\u30d7\u30c1\u30c9\u304a\u3088\u3073\u305d\u306e\u8a98\u5c0e\u4f53\uff08\u4f8b\uff1a\u30dd\u30ea\u30ea\u30b8\u30f3\u3001\u30dd\u30ea\u30aa\u30eb\u30cb\u30c1\u30f3\uff09\u3001\u76f4\u9396\u307e\u305f\u306f\u5206\u5c90\u9396\u306e\u5408\u6210\u30dd\u30ea\u30de\u30fc\uff08\u4f8b\uff1a \u30dd\u30ea\u30d6\u30ec\u30f3\u3001\u30dd\u30ea\u30a8\u30c1\u30ec\u30f3\u30a4\u30df\u30f3\uff09\u3001\u591a\u7cd6\u30d9\u30fc\u30b9\u306e\u5c0e\u5165\u5206\u5b50\uff08\u4f8b\uff1a \u30b7\u30af\u30ed\u30c7\u30ad\u30b9\u30c8\u30ea\u30f3\u3001\u30ad\u30c8\u30b5\u30f3\uff09\u3001\u5929\u7136\u30dd\u30ea\u30de\u30fc\uff08\u4f8b\uff1a \u30d2\u30b9\u30c8\u30f3\u3001\u30b3\u30e9\u30fc\u30b2\u30f3\uff09\u3001\u304a\u3088\u3073 \u6d3b\u6027\u578b\u304a\u3088\u3073\u975e\u6d3b\u6027\u578b\u30c7\u30f3\u30c9\u30ea\u30de\u30fc\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u3002<\/p>\n<p>\u30ab\u30c1\u30aa\u30f3\u6027\u30dd\u30ea\u30de\u30fc\u306f\u3001\u758e\u6c34\u6027\u90e8\u5206\u3092\u542b\u307e\u305a\u3001\u6c34\u306b\u5b8c\u5168\u306b\u6eb6\u89e3\u3059\u308b\u70b9\u3067\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u3068\u7570\u306a\u308a\u307e\u3059\u3002\u3053\u308c\u3089\u306f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304a\u3088\u3073\u7d30\u80de\u6bd2\u6027\u306e\u7a0b\u5ea6\u304c\u8457\u3057\u304f\u7570\u306a\u308a\u307e\u3059\u304c\u3001\u30ab\u30c1\u30aa\u30f3\u6027\u30dd\u30ea\u30de\u30fc\u306f\u5168\u3066\u3001\u6838\u9178-\u30dd\u30ea\u30de\u30fc\u8907\u5408\u4f53\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u540c\u69d8\u306e\u69d8\u5f0f\u3067\u4f5c\u7528\u3057\u307e\u3059\u3002\u8907\u5408\u4f53\u306f\u3001\u9759\u96fb\u76f8\u4e92\u4f5c\u7528\u3092\u4ecb\u3057\u3066\u7d30\u80de\u819c\u306b\u4ed8\u7740\u3057\u3001\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3092\u4ecb\u3057\u3066\u7d30\u80de\u306b\u53d6\u308a\u8fbc\u307e\u308c\u307e\u3059\u3002\u53d6\u308a\u8fbc\u307f\u52b9\u7387\u306f\u3001\u7d30\u80de\u6a19\u7684\u30ea\u30ac\u30f3\u30c9\u307e\u305f\u306f\u6838\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u3092\u30dd\u30ea\u30de\u30fc\u306b\u7d50\u5408\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u6539\u5584\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u30ab\u30c1\u30aa\u30f3\u6027\u30dd\u30ea\u30de\u30fc\u3067\u306f\u3001\u8907\u5408\u4f53\u306e\u5b89\u5b9a\u6027\u3001\u7d50\u679c\u306e\u518d\u73fe\u6027\u3001\u304a\u3088\u3073\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304cDEAE\u30c7\u30ad\u30b9\u30c8\u30e9\u30f3\u6cd5\u3088\u308a\u3082\u9ad8\u3081\u3089\u308c\u307e\u3059\u304c\u3001\u7d30\u80de\u6bd2\u6027\u3084\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u7814\u7a76\u306b\u9650\u5b9a\u3055\u308c\u308b\u3068\u3044\u3046\u4e3b\u8981\u306a\u5236\u9650\u306f\u540c\u3058\u304f\u5b58\u5728\u3057\u307e\u3059\u3002\u9ad8\u5206\u5b50\u91cf\u306e\u30ab\u30c1\u30aa\u30f3\u6027\u30dd\u30ea\u30de\u30fc\u306f\u3001\u975e\u751f\u5206\u89e3\u6027\u3067\u4f4e\u5206\u5b50\u30dd\u30ea\u30de\u30fc\u3088\u308a\u3082\u7d30\u80de\u6bd2\u6027\u304c\u9ad8\u3044\u50be\u5411\u304c\u3042\u308a\u3001\u30dd\u30ea\u30de\u30fc\uff0f\u6838\u9178\u306e\u96fb\u8377\u6bd4\u306e\u5897\u52a0\u306b\u3088\u3063\u3066\u9ad8\u3044\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3092\u793a\u3057\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u9ad8\u5206\u5b50\u30dd\u30ea\u30de\u30fc\u306e\u9ad8\u3044\u6bd2\u6027\u306f\u3001\u9ad8\u5206\u5b50\u69cb\u9020\u306b\u4f4e\u5206\u5b50\u30dd\u30ea\u30de\u30fc\u306b\u3088\u308b\u751f\u5206\u89e3\u6027\u67b6\u6a4b\u3092\u53d6\u308a\u5165\u308c\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u6e1b\u5c11\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<div id=\"attachment_1589\" style=\"width: 845px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig5.png\" rel=\"attachment wp-att-1589\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1589\" class=\"size-full wp-image-1589\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig5.png\" alt=\"gene-delivery-technologies-fig5\" width=\"835\" height=\"765\" \/><\/a><p id=\"caption-attachment-1589\" class=\"wp-caption-text\">\u56f35 \u30ab\u30c1\u30aa\u30f3\u6027\u30dd\u30ea\u30de\u30fc\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/p><\/div>\n<h2>\u751f\u7269\u5b66\u7684\u907a\u4f1d\u5b50\u5c0e\u5165\u6cd5<\/h2>\n<h3>\u30a6\u30a4\u30eb\u30b9\u306b\u3088\u308b\u5c0e\u5165<\/h3>\n<p>\u8102\u8cea\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u304c\u9069\u3055\u306a\u3044\u30bf\u30a4\u30d7\u306e\u7d30\u80de\u306b\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u304c\u3088\u304f\u7528\u3044\u3089\u308c\u307e\u3059\u3002<strong>\u30a6\u30a4\u30eb\u30b9\u5a92\u4ecb\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u5f62\u8cea\u5c0e\u5165\u3068\u3057\u3066\u3082\u77e5\u3089\u308c\u3001\u5c0e\u5165\u56f0\u96e3\u306a\u7d30\u80de\u30bf\u30a4\u30d7\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u904e\u5270\u767a\u73fe\u3084\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u306e\u305f\u3081\u306e\u65b9\u6cd5\u3068\u3057\u3066\u63d0\u4f9b\u3055\u308c\u3001\u81e8\u5e8a\u7814\u7a76\u306b\u304a\u3044\u3066\u6700\u3082\u4e00\u822c\u7684\u306b\u7528\u3044\u3089\u308c\u3066\u3044\u308b\u624b\u6cd5\u3067\u3059<\/strong>\uff08Glover et al., 2005; Pfeifer and Verma, 2001\uff09\u3002\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3001\u30aa\u30f3\u30b3\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9\u3001\u304a\u3088\u3073\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306f\u3001\u54fa\u4e73\u985e\u7d30\u80de\u57f9\u990a\u304a\u3088\u3073in vivo\u3067\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u306b\u5e45\u5e83\u304f\u7528\u3044\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u4ed6\u306e\u3088\u304f\u77e5\u3089\u308c\u305f\u30a6\u30a4\u30eb\u30b9\u907a\u4f1d\u5b50\u5c0e\u5165\u306e\u4f8b\u3068\u3057\u3066\u3001\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9\u304a\u3088\u3073\u30ef\u30af\u30b7\u30cb\u30a2\u30a6\u30a4\u30eb\u30b9\u30d9\u30fc\u30b9\u306e\u30d9\u30af\u30bf\u30fc\u306b\u3088\u308b\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u30a6\u30a4\u30eb\u30b9\u306f\u3001in vivo\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u9ad8\u304f\u3001\u5bbf\u4e3b\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u307e\u308c\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u907a\u4f1d\u5b50\u767a\u73fe\u304c\u7dad\u6301\u3055\u308c\u308b\u305f\u3081\u3001\u81e8\u5e8a\u8a66\u9a13\u306b\u304a\u3051\u308b\u907a\u4f1d\u5b50\u5c0e\u5165\u306e\u305f\u3081\u306e\u30b7\u30b9\u30c6\u30e0\u3068\u3057\u3066\u597d\u307e\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u3053\u306e\u624b\u6cd5\u306b\u306f\u514d\u75ab\u539f\u6027\u304a\u3088\u3073\u7d30\u80de\u6bd2\u6027\u3001\u6280\u8853\u7684\u306b\u56f0\u96e3\u3067\u6642\u9593\u3068\u52b4\u529b\u3092\u8981\u3059\u308b\u30d9\u30af\u30bf\u30fc\u306e\u88fd\u9020\u65b9\u6cd5\u3001\u30d0\u30a4\u30aa\u30bb\u30fc\u30d5\u30c6\u30a3\u30fc\u306e\u8981\u4ef6\u3092\u6e80\u305f\u3059\u305f\u3081\u306e\u9ad8\u3044\u30b3\u30b9\u30c8\u3001\u4f4e\u3044\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u5bb9\u91cf\uff08\u975e\u30a6\u30a4\u30eb\u30b9\u6027\u30d9\u30af\u30bf\u30fc\u304c100 kb\u307e\u3067\u3067\u3042\u308b\u306e\u306b\u5bfe\u3057\u3001\u5927\u90e8\u5206\u306e\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306f10 kb\u307e\u3067\uff09\u3001\u304a\u3088\u3073\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u8abf\u88fd\u7269\u306e\u611f\u67d3\u529b\u306e\u5909\u52d5\u306a\u3069\u306e\u6570\u591a\u304f\u306e\u6b20\u70b9\u304c\u3042\u308a\u307e\u3059\uff08Glover et al., 2005; Kim and Eberwine, 2010; Vorburger and Hunt, 2002\uff09\u3002<\/p>\n<p>\u4e00\u822c\u7684\u306a\u5f62\u8cea\u5c0e\u5165\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306b\u306f\u3001\u5c0e\u5165\u907a\u4f1d\u5b50\u3092\u904b\u3076\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u306e\u8a2d\u8a08\u3001\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u7d30\u80de\u7cfb\u306b\u304a\u3051\u308b\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u306e\u5897\u6b96\u3001\u5897\u6b96\u3055\u308c\u305f\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u306e\u7cbe\u88fd\u304a\u3088\u3073\u30bf\u30a4\u30c8\u30ec\u30fc\u30b7\u30e7\u30f3\u3001\u304a\u3088\u3073\u6b21\u306e\u76ee\u7684\u306e\u7d30\u80de\u3078\u306e\u611f\u67d3\u304c\u542b\u307e\u308c\u307e\u3059\u3002\u521d\u4ee3\u7d30\u80de\u304a\u3088\u3073\u7d30\u80de\u7cfb\u306b\u304a\u3051\u308b\u5f62\u8cea\u5c0e\u5165\u52b9\u7387\u304c\u6975\u3081\u3066\u9ad8\u3044\uff08\u7d0490\uff5e100%\uff09\u5834\u5408\u3001\u30a6\u30a4\u30eb\u30b9\u7279\u7570\u7684\u53d7\u5bb9\u4f53\u3092\u904b\u3076\u7d30\u80de\u306e\u307f\u304c\u30a6\u30a4\u30eb\u30b9\u306b\u611f\u67d3\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u30a6\u30a4\u30eb\u30b9\u5897\u5e45\u306b\u7528\u3044\u3089\u308c\u308b\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u7d30\u80de\u7cfb\u306f\u975e\u30a6\u30a4\u30eb\u30b9\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306b\u3088\u3063\u3066\u5c0e\u5165\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3053\u3068\u3092\u8a8d\u8b58\u3057\u3066\u304a\u304f\u3053\u3068\u306f\u91cd\u8981\u3067\u3059\u3002<\/p>\n<div id=\"attachment_1590\" style=\"width: 849px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig6.png\" rel=\"attachment wp-att-1590\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1590\" class=\"size-full wp-image-1590\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig6.png\" alt=\"gene-delivery-technologies-fig6\" width=\"839\" height=\"829\" \/><\/a><p id=\"caption-attachment-1590\" class=\"wp-caption-text\">\u56f36 \u30a6\u30a4\u30eb\u30b9\u5c0e\u5165\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/p><\/div>\n<h4>\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306e\u57fa\u672c\u7279\u6027<\/h4>\n<p>\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306f\u3001\u7279\u5b9a\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u5408\u308f\u305b\u3066\u4f5c\u88fd\u3057\u307e\u3059\u304c\u3001\u901a\u5e38\u3001\u3044\u304f\u3064\u304b\u306e\u57fa\u672c\u7279\u6027\u306f\u5171\u901a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<ul>\n<li><strong>\u5b89\u5168\u6027\uff1a<\/strong> \u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306f\u3001\u591a\u304f\u306e\u5834\u5408\u3001\u75c5\u539f\u6027\u30a6\u30a4\u30eb\u30b9\u304b\u3089\u4f5c\u88fd\u3055\u308c\u307e\u3059\u304c\u3001\u53d6\u308a\u6271\u3046\u969b\u306e\u30ea\u30b9\u30af\u3092\u6700\u5c0f\u5316\u3059\u308b\u3088\u3046\u306b\u6539\u5909\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u6539\u5909\u306b\u306f\u3001\u901a\u5e38\u3001\u30a6\u30a4\u30eb\u30b9\u306e\u8907\u88fd\u306b\u5fc5\u8981\u4e0d\u53ef\u6b20\u306a\u30a6\u30a4\u30eb\u30b9\u30b2\u30ce\u30e0\u306e\u4e00\u90e8\u306e\u6b20\u5931\u304c\u542b\u307e\u308c\u3001\u30a6\u30a4\u30eb\u30b9\u304c\u7d30\u80de\u306b\u52b9\u7387\u826f\u304f\u611f\u67d3\u3057\u3066\u5c0e\u5165\u3055\u308c\u308b\u3053\u3068\u306f\u53ef\u80fd\u3068\u3057\u307e\u3059\u304c\u3001\u6b20\u640d\u3057\u3066\u3044\u308b\u30b2\u30ce\u30e0\u306e\u8907\u88fd\u306b\u5fc5\u8981\u4e0d\u53ef\u6b20\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f9b\u7d66\u3059\u308b\u30d8\u30eb\u30d1\u30fc\u30a6\u30a4\u30eb\u30b9\u306e\u975e\u5b58\u5728\u4e0b\u3067\u306e\u65b0\u3057\u3044\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u306e\u7523\u751f\u306f\u59a8\u3052\u3089\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u4f7f\u7528\u306e\u5b89\u5168\u6027\u306b\u3064\u3044\u3066\u306f\u3001\u816b\u760d\u6291\u5236\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u59a8\u3052\u308b\u3001\u3042\u308b\u3044\u306f\u7d30\u80de\u306e\u60aa\u6027\u5f62\u8cea\u8ee2\u63db\u3092\u5c0e\u304f\u764c\u907a\u4f1d\u5b50\u3092\u6d3b\u6027\u5316\u3059\u308b\u30a6\u30a4\u30eb\u30b9DNA\u306e\u7570\u6240\u6027\u67d3\u8272\u4f53\u7d44\u307f\u8fbc\u307f\u3092\u751f\u3058\u3055\u305b\u308b\u633f\u5165\u5909\u7570\u306b\u5bfe\u3059\u308b\u61f8\u5ff5\u306f\u5f15\u304d\u7d9a\u304d\u5b58\u5728\u3057\u307e\u3059\uff08Glover et al., 2005\uff09\u3002<\/li>\n<li><strong>\u4f4e\u6bd2\u6027\uff1a<\/strong> \u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u304c\u611f\u67d3\u3057\u305f\u7d30\u80de\u306e\u751f\u7406\u6a5f\u80fd\u306b\u4e0e\u3048\u308b\u5f71\u97ff\u306f\u6700\u5c0f\u9650\u306b\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u306fin vivo\u3067\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u3092\u5fc5\u8981\u3068\u3059\u308b\u7814\u7a76\u306b\u304a\u3044\u3066\u7279\u306b\u91cd\u8981\u3067\u3059\u3002\u306a\u305c\u306a\u3089\u3001\u3082\u3057\u30d9\u30af\u30bf\u30fc\u304c\u5916\u6765\u4fb5\u5165\u7269\u3068\u307f\u306a\u3055\u308c\u308b\u3068\u514d\u75ab\u53cd\u5fdc\u304c\u8a98\u5c0e\u3055\u308c\u308b\u304b\u3089\u3067\u3059\uff08Nayak and Herzog, 2009\uff09<\/li>\n<li><strong>\u5b89\u5b9a\u6027\uff1a<\/strong> \u4e00\u90e8\u306e\u30a6\u30a4\u30eb\u30b9\u306f\u907a\u4f1d\u7684\u306b\u4e0d\u5b89\u5b9a\u3067\u3001\u6025\u901f\u306b\u30b2\u30ce\u30e0\u518d\u7de8\u6210\u304c\u8d77\u304d\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u3066\u5b9f\u65bd\u3057\u305f\u7814\u7a76\u306e\u4e88\u6e2c\u6027\u304a\u3088\u3073\u518d\u73fe\u6027\u306b\u5f0a\u5bb3\u3092\u3082\u305f\u3089\u3057\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u4e0d\u5b89\u5b9a\u306a\u30d9\u30af\u30bf\u30fc\u306f\u901a\u5e38\u907f\u3051\u3089\u308c\u307e\u3059\u3002<\/li>\n<li><strong>\u7d30\u80de\u30bf\u30a4\u30d7\u306e\u7279\u7570\u6027\uff1a<\/strong> 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et al.,1997\uff09\u3002CAR\u3068\u7d50\u5408\u3057\u305f\u5f8c\u3001\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u30a4\u30f3\u30c6\u30b0\u30ea\u30f3\u3092\u4ecb\u3057\u305f\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3092\u7d4c\u3066\u5185\u5728\u5316\u3057\u3001\u7d9a\u3044\u3066\u6838\u306b\u80fd\u52d5\u8f38\u9001\u3055\u308c\u3066\u3001\u305d\u306eDNA\u304c\u30a8\u30d4\u30bd\u30fc\u30e0\u3068\u3057\u3066\u767a\u73fe\u3055\u308c\u307e\u3059\uff08Hirata and Russell, 2000\uff09\u3002\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306f\u591a\u304f\u306e\u7d30\u80de\u30bf\u30a4\u30d7\u306b\u304a\u3051\u308b\u4e00\u904e\u6027\u5c0e\u5165\u306b\u304a\u3044\u3066\u3046\u307e\u304f\u6a5f\u80fd\u3057\u307e\u3059\u304c\u3001\u975e\u5206\u88c2\u7d30\u80de\u306a\u3069\u306e\u4e00\u90e8\u306e\u5c0e\u5165\u56f0\u96e3\u306a\u7d30\u80de\u7cfb\u306e\u5c0e\u5165\u3084\u5b89\u5b9a\u767a\u73fe\u306b\u306f\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u304c\u9069\u3057\u307e\u3059\u3002\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u5bb9\u91cf\u306f7\uff5e8 kb\u3067\u3059\u3002<\/p>\n<p><strong>\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9<\/strong>\u306f\u3001\u5bbf\u4e3b\u7d30\u80de\u306e\u67d3\u8272\u4f53\u306b\u81ea\u3089\u306e\u30b2\u30ce\u30e0\u3092\u5b89\u5b9a\u7684\u306b\u7d44\u307f\u8fbc\u307e\u305b\u308b\u30d7\u30e9\u30b9\u9396RNA\u30a6\u30a4\u30eb\u30b9\u3067\u3059\u3002\u30a8\u30f3\u30d9\u30ed\u30fc\u30d7\u3092\u6c34\u75b1\u6027\u53e3\u5185\u708e\u30a6\u30a4\u30eb\u30b9\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\uff08VSV G\uff09\u306a\u3069\u3067\u507d\u578b\u5316\u3059\u308b\u3068\u3001\u5e45\u5e83\u3044\u6307\u5411\u6027\u304c\u5099\u308f\u308a\u3001\u57fa\u672c\u7684\u306b\u3042\u3089\u3086\u308b\u30bf\u30a4\u30d7\u306e\u54fa\u4e73\u985e\u7d30\u80de\u306b\u4fb5\u5165\u3055\u305b\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u5927\u90e8\u5206\u306e\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u7d30\u80de\u5206\u88c2\u4e2d\u306e\u6838\u819c\u306e\u5d29\u58ca\u306b\u4f34\u3044\u7d30\u80de\u306b\u611f\u67d3\u3059\u308b\u305f\u3081\u3001\u305d\u306e\u4f7f\u7528\u306f\u5f62\u8cea\u5c0e\u5165\u306b\u304a\u3051\u308b\u7d30\u80de\u306e\u8907\u88fd\u306e\u5fc5\u8981\u6027\u306b\u3088\u3063\u3066\u5236\u9650\u3055\u308c\u307e\u3059\u3002\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9\u306e\u4ed6\u306e\u6b20\u70b9\u3068\u3057\u3066\u3001\u633f\u5165\u5909\u7570\u306e\u53ef\u80fd\u6027\u304a\u3088\u3073\u6f5c\u5728\u7684\u75be\u60a3\u306e\u6d3b\u6027\u5316\u306e\u53ef\u80fd\u6027\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u3002\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3068\u540c\u69d8\u306b\u3001\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9\u306f\u7d048 kb\u306e\u5916\u6765\u907a\u4f1d\u5b50\u3092\u633f\u5165\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p><strong>\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9<\/strong>\u306f\u3001\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u30b5\u30d6\u30b0\u30eb\u30fc\u30d7\u3067\u3042\u308b\u305f\u3081\u3001\u5bbf\u4e3b\u7d30\u80de\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u3093\u3067\u3001\u5b89\u5b9a\u7684\u306a\u9577\u671f\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u63d0\u4f9b\u3067\u304d\u307e\u3059\uff08Anson, 2004\uff09\u3002\u4ed6\u306e\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9\u3068\u306f\u5927\u304d\u304f\u7570\u306a\u308a\u3001\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9\u306f\u3088\u308a\u591a\u7528\u9014\u306b\u4f7f\u7528\u3067\u304d\u308b\u30c4\u30fc\u30eb\u3067\u3001\u6d3b\u6027\u5316\u3055\u308c\u305f\u6838\u8f38\u9001\u7d4c\u8def\u3092\u7528\u3044\u3066\u3001\u795e\u7d4c\u7d30\u80de\u3084\u9020\u8840\u7d30\u80de\u306a\u3069\u306e\u975e\u5206\u88c2\u6700\u7d42\u5206\u5316\u7d30\u80de\u306b\u5f62\u8cea\u5c0e\u5165\u3067\u304d\u307e\u3059\u3002<\/p>\n<p><strong>\u30a2\u30c7\u30ce\u968f\u4f34\u30a6\u30a4\u30eb\u30b9<\/strong>\u306f\u3001\u5e45\u5e83\u3044\u30bf\u30a4\u30d7\u306e\u5206\u88c2\u7d30\u80de\u304a\u3088\u3073\u975e\u5206\u88c2\u7d30\u80de\u306b\u5f62\u8cea\u5c0e\u5165\u3067\u304d\u307e\u3059\u304c\u3001\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u7d30\u80de\u306b\u304a\u3044\u3066\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u3092\u7523\u751f\u3055\u305b\u308b\u305f\u3081\u306e\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3084\u30d8\u30eb\u30da\u30b9\u30a6\u30a4\u30eb\u30b9\u306a\u3069\u306e\u30d8\u30eb\u30d1\u30fc\u30a6\u30a4\u30eb\u30b9\u3068\u306e\u5171\u611f\u67d3\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u624b\u6cd5\u3067\u306f\u3001\u30d8\u30eb\u30d1\u30fc\u30a6\u30a4\u30eb\u30b9\u3092\u542b\u307e\u306a\u3044\u9ad8\u54c1\u8cea\u306e\u30a6\u30a4\u30eb\u30b9\u30b9\u30c8\u30c3\u30af\u3092\u7372\u5f97\u3059\u308b\u3053\u3068\u306b\u56f0\u96e3\u304c\u751f\u3058\u307e\u3059\u3002\u307e\u305f\u3001\u30a2\u30c7\u30ce\u968f\u4f34\u30a6\u30a4\u30eb\u30b9\u306e\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u5bb9\u91cf\u306f\u308f\u305a\u304b4.9 kb\u307e\u3067\u3068\u9650\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u30a2\u30c7\u30ce\u968f\u4f34\u30a6\u30a4\u30eb\u30b9\u306f\u5927\u90e8\u5206\u306e\u7d30\u80de\u30bf\u30a4\u30d7\u306b\u304a\u3044\u3066\u4f4e\u3044\u514d\u75ab\u539f\u6027\u3092\u793a\u3057\u3001\u30d2\u30c8\u67d3\u8272\u4f53\u306e\u7279\u5b9a\u306e\u9818\u57df\u306b\u7d44\u307f\u8fbc\u307e\u308c\u308b\u305f\u3081\u3001\u633f\u5165\u5909\u7570\u3092\u56de\u907f\u3067\u304d\u307e\u3059\u3002<\/p>\n<p><strong>\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u904e\u5270\u767a\u73fe\u306b\u4f7f\u7528\u3067\u304d\u308b\u4ed6\u306e\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u30b7\u30b9\u30c6\u30e0<\/strong>\u306b\u306f\u3001<strong>\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9<\/strong>\u3001<strong>\u30ef\u30af\u30b7\u30cb\u30a2\u30a6\u30a4\u30eb\u30b9<\/strong>\u3001\u304a\u3088\u3073<strong>\u5358\u7d14\u30d8\u30eb\u30da\u30b9\u30a6\u30a4\u30eb\u30b9<\/strong>\u3092\u30d9\u30fc\u30b9\u3068\u3059\u308b\u30d9\u30af\u30bf\u30fc\u304c\u3042\u308a\u307e\u3059\u3002\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u901a\u5e38\u6606\u866b\u7d30\u80de\u306b\u611f\u67d3\u3057\u307e\u3059\u304c\u3001\u7d44\u63db\u3048\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u5e83\u7bc4\u306a\u54fa\u4e73\u985e\u7d30\u80de\u30bf\u30a4\u30d7\u306b\u304a\u3051\u308b\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e00\u904e\u6027\u767a\u73fe\u306e\u305f\u3081\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u5a92\u4ecb\u7269\u3068\u3057\u3066\u6a5f\u80fd\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306b\u512a\u6027\u9078\u629e\u30de\u30fc\u30ab\u30fc\u3092\u542b\u307e\u305b\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u7cfb\u304c\u5b89\u5b9a\u7684\u306b\u7d44\u63db\u3048\u907a\u4f1d\u5b50\u3092\u767a\u73fe\u3059\u308b\u3088\u3046\u306b\u8a98\u5c0e\u3067\u304d\u307e\u3059\uff08Condreay et al., 1999\uff09\u3002\u30ef\u30af\u30b7\u30cb\u30a2\u30a6\u30a4\u30eb\u30b9\u3092\u30d9\u30fc\u30b9\u3068\u3059\u308b\u30d9\u30af\u30bf\u30fc\u306f\u3001\u5927\u304d\u3044DNA\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306e\u5e45\u5e83\u3044\u54fa\u4e73\u985e\u7d30\u80de\u3078\u306e\u5c0e\u5165\u306b\u7528\u3044\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u30ef\u30af\u30b7\u30cb\u30a2\u30a6\u30a4\u30eb\u30b9\u304c\u611f\u67d3\u3057\u305f\u7d30\u80de\u306f1\uff5e2\u65e5\u4ee5\u5185\u306b\u6b7b\u6ec5\u3059\u308b\u305f\u3081\u3001\u3053\u306e\u30b7\u30b9\u30c6\u30e0\u306e\u4f7f\u7528\u306f\u4e00\u904e\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u751f\u7523\u306b\u9650\u5b9a\u3055\u308c\u307e\u3059\u3002\u5358\u7d14\u30d8\u30eb\u30da\u30b9\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u795e\u7d4c\u7d30\u80de\u306b\u611f\u67d3\u3059\u308b\u4e8c\u672c\u9396DNA\u30bf\u30a4\u30d7\u306e\u30a6\u30a4\u30eb\u30b9\u3067\u3059\u3002<\/p>\n<table>\n<caption>\u8868 \u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306e\u7a2e\u985e\u3068\u7279\u5fb4<\/caption>\n<tbody>\n<tr>\n<th>\u30a6\u30a4\u30eb\u30b9\u30b7\u30b9\u30c6\u30e0<\/th>\n<th>\u30b5\u30a4\u30ba<\/th>\n<th>DNA\u30a4\u30f3\u30b5\u30fc\u30c8\u30b5\u30a4\u30ba<\/th>\n<th>\u6700\u5927\u529b\u4fa1(\u7c92\u5b50\/mL)<\/th>\n<th>\u611f\u67d3<\/th>\n<th>\u767a\u73fe<\/th>\n<th>\u6b20\u70b9<\/th>\n<\/tr>\n<tr>\n<td>\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9<\/td>\n<td>36 kb (dsDNA)<\/td>\n<td>8 kb<\/td>\n<td>1 \u00d7 10<sup>13<\/sup><\/td>\n<td>\u5206\u88c2\u7d30\u80de\u304a\u3088\u3073\u975e\u5206\u88c2\u7d30\u80de<\/td>\n<td>\u4e00\u904e\u6027<\/td>\n<td>\u5f37\u529b\u306a\u6297\u30a6\u30a4\u30eb\u30b9\u514d\u75ab\u53cd\u5fdc\u3092\u8a98\u767a<\/td>\n<\/tr>\n<tr>\n<td>\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9<\/td>\n<td>7\u201311 kb (ssRNA)<\/td>\n<td>8 kb<\/td>\n<td>1 \u00d7 10<sup>9<\/sup><\/td>\n<td>\u5206\u88c2\u7d30\u80de<\/td>\n<td>\u5b89\u5b9a<\/td>\n<td>\u633f\u5165\u5909\u7570\u306e\u53ef\u80fd\u6027<\/td>\n<\/tr>\n<tr>\n<td>\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9<\/td>\n<td>8 kb (ssRNA)<\/td>\n<td>9 kb<\/td>\n<td>1 \u00d7 10<sup>9<\/sup><\/td>\n<td>\u5206\u88c2\u7d30\u80de\u304a\u3088\u3073\u975e\u5206\u88c2\u7d30\u80de<\/td>\n<td>\u5b89\u5b9a<\/td>\n<td>\u633f\u5165\u5909\u7570\u306e\u53ef\u80fd\u6027<\/td>\n<\/tr>\n<tr>\n<td>\u30a2\u30c7\u30ce\u968f\u4f34\u30a6\u30a4\u30eb\u30b9<\/td>\n<td>8.5 kb (ssDNA)<\/td>\n<td>5 kb<\/td>\n<td>1 \u00d7 10<sup>11<\/sup><\/td>\n<td>\u5206\u88c2\u7d30\u80de\u304a\u3088\u3073\u975e\u5206\u88c2\u7d30\u80de<\/td>\n<td>\u5b89\u5b9a: \u90e8\u4f4d\u7279\u7570\u7684\u306a\u7d44\u307f\u8fbc\u307f<\/td>\n<td>\u8907\u88fd\u306b\u306f\u30d8\u30eb\u30d1\u30fc\u30a6\u30a4\u30eb\u30b9\u304c\u5fc5\u8981: \u9ad8\u7d14\u5ea6\u306a\u30a6\u30a4\u30eb\u30b9\u30b9\u30c8\u30c3\u30af\u306e\u7523\u751f\u304c\u56f0\u96e3<\/td>\n<\/tr>\n<tr>\n<td>\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9<\/td>\n<td>80\u2013180 kb (dsDNA)<\/td>\n<td>\u4e0a\u9650\u306f\u4e0d\u660e<\/td>\n<td>1 \u00d7 10<sup>8<\/sup><\/td>\n<td>\u5206\u88c2\u7d30\u80de\u304a\u3088\u3073\u975e\u5206\u88c2\u7d30\u80de<\/td>\n<td>\u4e00\u904e\u6027\u307e\u305f\u306f\u5b89\u5b9a<\/td>\n<td>\u9650\u5b9a\u3055\u308c\u305f\u54fa\u4e73\u985e\u5bbf\u4e3b\u7bc4\u56f2<\/td>\n<\/tr>\n<tr>\n<td>\u30ef\u30af\u30b7\u30cb\u30a2\u30a6\u30a4\u30eb\u30b9<\/td>\n<td>190 kb (dsDNA)<\/td>\n<td>25 kb<\/td>\n<td>1 \u00d7 10<sup>9<\/sup><\/td>\n<td>\u5206\u88c2\u7d30\u80de<\/td>\n<td>\u4e00\u904e\u6027<\/td>\n<td>\u7d30\u80de\u5909\u6027\u52b9\u679c\u306e\u53ef\u80fd\u6027<\/td>\n<\/tr>\n<tr>\n<td>\u5358\u7d14\u30d8\u30eb\u30da\u30b9\u30a6\u30a4\u30eb\u30b9<\/td>\n<td>150 kb (dsDNA)<\/td>\n<td>30\u201340 kb<\/td>\n<td>1 \u00d7 10<sup>9<\/sup><\/td>\n<td>\u5206\u88c2\u7d30\u80de\u304a\u3088\u3073\u975e\u5206\u88c2\u7d30\u80de<\/td>\n<td>\u4e00\u904e\u6027<\/td>\n<td>\u6f5c\u4f0f\u611f\u67d3\u6642\u306b\u306f\u907a\u4f1d\u5b50\u304c\u767a\u73fe\u3057\u306a\u3044<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u7269\u7406\u7684\u907a\u4f1d\u5b50\u5c0e\u5165\u6cd5<\/h2>\n<h3>\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3<\/h3>\n<p><strong>\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u306f\u3001\u96fb\u6c17\u30d1\u30eb\u30b9\u3092\u7528\u3044\u3066\u4e00\u6642\u7684\u306b\u7d30\u80de\u819c\u306b\u5b54\u3092\u5f62\u6210\u3057\u3001\u305d\u306e\u5b54\u3092\u4ecb\u3057\u3066\u3001\u6838\u9178\u306e\u3088\u3046\u306a\u7269\u8cea\u3092\u7d30\u80de\u5185\u306b\u901a\u904e\u3055\u305b\u308b\u7269\u7406\u7684\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3067\u3059\u3002<\/strong>\u3053\u308c\u306f\u3001\u5916\u6765\u6838\u9178\u3092\u7d30\u83cc\u7d30\u80de\u3084\u54fa\u4e73\u985e\u7d30\u80de\u3092\u542b\u3080\u591a\u304f\u306e\u30bf\u30a4\u30d7\u306e\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u305f\u3081\u306e\u975e\u5e38\u306b\u52b9\u7387\u7684\u306a\u6226\u7565\u3067\u3059\u3002<\/p>\n<p>\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u306f\u30b7\u30f3\u30d7\u30eb\u306a\u30d7\u30ed\u30bb\u30b9\u306b\u57fa\u3065\u304d\u307e\u3059\u3002\u5bbf\u4e3b\u7d30\u80de\u304a\u3088\u3073\u9078\u629e\u3055\u308c\u305f\u5206\u5b50\u3092\u5c0e\u96fb\u6027\u6eb6\u6db2\u306b\u61f8\u6fc1\u3057\u3001\u6df7\u5408\u6db2\u5468\u56f2\u306e\u96fb\u6c17\u56de\u8def\u3092\u9589\u3058\u307e\u3059\u3002\u7d30\u80de\u61f8\u6fc1\u6db2\u306b\u3001\u6700\u9069\u306a\u96fb\u5727\u3067\u3001\u308f\u305a\u304b\u6570\u30de\u30a4\u30af\u30ed\u79d2\u304b\u3089\u30df\u30ea\u79d2\u7d99\u7d9a\u3059\u308b\u96fb\u6c17\u30d1\u30eb\u30b9\u3092\u304b\u3051\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u819c\u306e\u30ea\u30f3\u8102\u8cea\u4e8c\u91cd\u819c\u304c\u7834\u58ca\u3055\u308c\u3001\u4e00\u6642\u7684\u306b\u5b54\u304c\u5f62\u6210\u3055\u308c\u307e\u3059\u3002\u540c\u6642\u306b\u7d30\u80de\u819c\u3068\u7d30\u80de\u8cea\u306e\u9593\u306e\u96fb\u4f4d\u5dee\u304c\u5897\u5927\u3057\u3001DNA\u306e\u3088\u3046\u306a\u96fb\u8377\u3092\u6301\u3064\u5206\u5b50\u304c\u96fb\u6c17\u6cf3\u52d5\u3068\u540c\u69d8\u306e\u69d8\u5f0f\u3067\u7d30\u80de\u819c\u3092\u901a\u904e\u3057\u307e\u3059\uff08Shigekawa and Dower, 1988\uff09\u3002<\/p>\n<p><strong>\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u4e3b\u8981\u306a\u5229\u70b9<\/strong>\u306f\u3001\u5168\u3066\u306e\u7d30\u80de\u30bf\u30a4\u30d7\u306e\u4e00\u904e\u6027\u304a\u3088\u3073\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u9069\u7528\u53ef\u80fd\u306a\u70b9\u3067\u3059\u3002\u307e\u305f\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u306f\u7c21\u5358\u3067\u8fc5\u901f\u306a\u305f\u3081\u3001\u4e00\u5ea6\u6700\u9069\u306a\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6761\u4ef6\u304c\u6c7a\u5b9a\u3055\u308c\u308b\u3068\u3001\u77ed\u6642\u9593\u3067\u6570\u591a\u304f\u306e\u5c0e\u5165\u304c\u884c\u3048\u307e\u3059\u3002<strong>\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u4e3b\u8981\u306a\u6b20\u70b9<\/strong>\u306f\u3001\u9ad8\u96fb\u5727\u30d1\u30eb\u30b9\u306b\u3088\u3063\u3066\u76f8\u5f53\u6570\u306e\u7d30\u80de\u6b7b\u304c\u5f15\u304d\u8d77\u3053\u3055\u308c\u3001\u7d30\u80de\u819c\u306f\u90e8\u5206\u7684\u306b\u3057\u304b\u4fee\u5fa9\u3055\u308c\u306a\u3044\u305f\u3081\u3001\u5316\u5b66\u7684\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306b\u6bd4\u3079\u3066\u591a\u91cf\u306e\u7d30\u80de\u304c\u5fc5\u8981\u3068\u3055\u308c\u308b\u70b9\u3067\u3059\u3002\u5f0a\u793e\u306e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-culture\/transfection\/neon-transfection-system.html?CID=bid_clb_tfx_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cc13_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Invitrogen\u2122 Neon\u2122 Transfection System<\/a>\u306e\u3088\u3046\u306b\u30d4\u30da\u30c3\u30c8\u30c1\u30c3\u30d7\u578b\u96fb\u6975\u3092\u63a1\u7528\u3057\u305f\u88c5\u7f6e\u3067\u306f\u3001\u96fb\u6c17\u30d1\u30eb\u30b9\u3092\u7d30\u80de\u4e2d\u306b\u5747\u4e00\u306b\u5206\u914d\u3057\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u30c1\u30e3\u30f3\u30d0\u30fc\u5168\u4f53\u3092\u5b89\u5b9a\u306apH\u306b\u7dad\u6301\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u9ad8\u3044\u7d30\u80de\u6b7b\u4ea1\u7387\u304c\u514b\u670d\u3055\u308c\u307e\u3059\u304c\u3001\u305d\u308c\u3067\u3082\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u52b9\u7387\u3068\u7d30\u80de\u751f\u5b58\u7387\u306e\u30d0\u30e9\u30f3\u30b9\u3092\u53d6\u308b\u305f\u3081\u306e\u30d1\u30eb\u30b9\u304a\u3088\u3073\u96fb\u754c\u5f37\u5ea6\u30d1\u30e9\u30e1\u30fc\u30bf\u306e\u6700\u9069\u5316\u306f\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002<\/p>\n<div id=\"attachment_1593\" style=\"width: 847px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig7.png\" rel=\"attachment wp-att-1593\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1593\" class=\"size-full wp-image-1593\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/gene-delivery-technologies-fig7.png\" alt=\"gene-delivery-technologies-fig7\" width=\"837\" height=\"695\" \/><\/a><p id=\"caption-attachment-1593\" class=\"wp-caption-text\">\u56f37 \u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/p><\/div>\n<h4>Neon Transfection System\u3068\u306f<\/h4>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-culture\/transfection\/neon-transfection-system.html?CID=bid_clb_tfx_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cc13_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Invitrogen\u2122 Neon\u2122 Transfection System<\/a>\u306f\u3001\u5f0a\u793e\u304c\u63d0\u4f9b\u3059\u308b\u30d9\u30f3\u30c1\u30c8\u30c3\u30d7\u578b\u306e\u6b21\u4e16\u4ee3\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u88c5\u7f6e\u3067\u3001<strong>\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u30c1\u30e3\u30f3\u30d0\u30fc\u3068\u3057\u3066\u306e\u30d4\u30da\u30c3\u30c8\u30c1\u30c3\u30d7\u578b\u96fb\u6975\u306b\u3088\u3063\u3066\u3001\u521d\u4ee3\u304a\u3088\u3073\u4e0d\u6b7b\u5316\u9020\u8840\u7d30\u80de\u3092\u542b\u3080\u54fa\u4e73\u985e\u7d30\u80de\u3001\u5e79\u7d30\u80de\u3001\u306a\u3089\u3073\u306b\u521d\u4ee3\u7d30\u80de\u3078\u306e\u52b9\u7387\u7684\u306a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3092\u53ef\u80fd\u3068\u3057\u307e\u3059\u3002<\/strong>\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u30c1\u30e3\u30f3\u30d0\u30fc\u306f\u3001\u7d30\u80de\u9593\u306b\u5747\u8cea\u306a\u96fb\u754c\u3092\u751f\u3058\u3001\u30c1\u30e3\u30f3\u30d0\u30fc\u5168\u4f53\u3067\u5b89\u5b9a\u306apH\u3092\u7dad\u6301\u3067\u304d\u308b\u3088\u3046\u306b\u8a2d\u8a08\u3055\u308c\u3066\u304a\u308a\u3001\u305d\u308c\u306b\u3088\u3063\u3066\u30a4\u30aa\u30f3\u306e\u751f\u6210\u3068\u3054\u304f\u308f\u305a\u304b\u306a\u71b1\u306e\u751f\u6210\u304c\u6291\u3048\u3089\u308c\u3001\u30ad\u30e5\u30d9\u30c3\u30c8\u3092\u7528\u3044\u308b\u5f93\u6765\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u30c1\u30e3\u30f3\u30d0\u30fc\u306b\u6bd4\u3079\u3066\u7d30\u80de\u751f\u5b58\u7387\u3068\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u5897\u52a0\u3057\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-culture\/transfection\/neon-transfection-system.html?CID=bid_clb_tfx_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cc13_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Neon Transfection System<\/a>\u306f\u3001\u6838\u9178\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u304a\u3088\u3073siRNA\u3092\u521d\u4ee3\u7d30\u80de\u304a\u3088\u3073\u5e79\u7d30\u80de\u3092\u542b\u3080\u3042\u3089\u3086\u308b\u30bf\u30a4\u30d7\u306e\u54fa\u4e73\u985e\u7d30\u80de\u306b\u52b9\u7387\u826f\u304f\u3001\u9ad8\u3044\u7d30\u80de\u751f\u5b58\u7387\u3067\u5c0e\u5165\u3057\u307e\u3059\u3002\u69d8\u3005\u306a\u7d30\u80de\u57f9\u990a\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff0860 mm\u30016 well\u300148 well\u304a\u3088\u307324 well\uff09\u306b\u3066\u300110 \u03bcL\u307e\u305f\u306f100 \u03bcL\u306e\u30b5\u30f3\u30d7\u30eb\u5bb9\u91cf\u3092\u7528\u3044\u3066\u30011\u56de\u306e\u53cd\u5fdc\u30671\u00d710<sup>4<\/sup>\u500b\u304b\u30895\u00d710<sup>6<\/sup>\u500b\u306e\u7d30\u80de\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-culture\/transfection\/neon-transfection-system.html?CID=bid_clb_tfx_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cc13_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Neon Transfection System<\/a>\u306b\u306f\u3001\u521d\u4ee3\u7d30\u80de\u304a\u3088\u3073\u5e79\u7d30\u80de\u3092\u542b\u3080\u69d8\u3005\u306a\u30bf\u30a4\u30d7\u306e\u54fa\u4e73\u985e\u7d30\u80de\u306b\u5bfe\u5fdc\u3067\u304d\u308b1\u7a2e\u985e\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u30ad\u30c3\u30c8\uff08Neon\u2122 Kit\uff09\u304c\u4f7f\u7528\u3055\u308c\u308b\u305f\u3081\u3001\u7d30\u80de\u306e\u30bf\u30a4\u30d7\u3054\u3068\u306b\u30d0\u30c3\u30d5\u30a1\u30fc\u30ad\u30c3\u30c8\u3092\u9078\u3093\u3067\u8cfc\u5165\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u307e\u305f\u3001Neon\u88c5\u7f6e\u306b\u306f\u6838\u9178\uff0fsiRNA\u304a\u3088\u3073\u7d30\u80de\u30bf\u30a4\u30d7\u306e24\u30a6\u30a7\u30eb\u7528\u306e\u6700\u9069\u5316\u6761\u4ef6\u691c\u8a0e\u7528\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u304c\u4e88\u3081\u30a4\u30f3\u30b9\u30c8\u30fc\u30eb\u3055\u308c\u3066\u304a\u308a\u3001Neon\u88c5\u7f6e\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306b\u306f\u6700\u592750\u7a2e\u985e\u306e\u7d30\u80de\u306e\u305f\u3081\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306e\u30d7\u30ed\u30b0\u30e9\u30df\u30f3\u30b0\u304a\u3088\u3073\u4fdd\u5b58\u304c\u53ef\u80fd\u3067\u3059\u3002\u6700\u9069\u5316\u3055\u308c\u305f\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-culture\/transfection\/neon-transfection-system.html?CID=bid_clb_tfx_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cc13_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u3053\u3061\u3089<\/a>\u304b\u3089\u4fbf\u5229\u306b\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u3001\u591a\u304f\u306e\u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u308b\u7d30\u80de\u30bf\u30a4\u30d7\u306b\u304a\u3051\u308b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3092\u6700\u5927\u5316\u3067\u304d\u307e\u3059\u3002<\/p>\n<h3>\u305d\u306e\u4ed6\u306e\u7269\u7406\u7684\u5c0e\u5165\u6cd5<\/h3>\n<p>\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u4ee5\u5916\u306e\u7269\u7406\u7684\u907a\u4f1d\u5b50\u5c0e\u5165\u6cd5\u306b\u306f\u3001<strong>\u907a\u4f1d\u5b50\u9283\u306b\u3088\u308b\u5fae\u7c92\u5b50\u306e\u5c0e\u5165<\/strong>\u3001<strong>\u76f4\u63a5\u30de\u30a4\u30af\u30ed\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3<\/strong>\u3001\u304a\u3088\u3073<strong>\u30ec\u30fc\u30b6\u30fc\u6cd5\u306b\u3088\u308b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3<\/strong>\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u7269\u7406\u7684\u624b\u6cd5\u306f\u4f7f\u7528\u3059\u308b\u30c4\u30fc\u30eb\u304c\u305d\u308c\u305e\u308c\u7570\u306a\u308a\u307e\u3059\u304c\u3001\u3053\u308c\u3089\u306f\u5168\u3066\u3001\u5316\u5b66\u7684\u624b\u6cd5\u3084\u30a6\u30a4\u30eb\u30b9\u7684\u624b\u6cd5\u3092\u4f7f\u7528\u305b\u305a\u306b\u7d30\u80de\u819c\u3092\u901a\u904e\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u308b\u6838\u9178\u306e\u7d30\u80de\u8cea\u307e\u305f\u306f\u6838\u3078\u306e\u76f4\u63a5\u5c0e\u5165\u3092\u53ef\u80fd\u3068\u3057\u307e\u3059\u3002<\/p>\n<p>\u7aef\u7684\u306b\u8ff0\u3079\u308b\u3068\u3001<strong>\u907a\u4f1d\u5b50\u9283\u306b\u3088\u308b\u5fae\u7c92\u5b50\u306e\u5c0e\u5165<\/strong>\u306f\u3001\u7c92\u5b50\u7167\u5c04\u3068\u3057\u3066\u3082\u77e5\u3089\u308c\u3001\u9855\u5fae\u93e1\u4e0b\u3067\u6838\u9178\u3092\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u3057\u305f\u91cd\u91d1\u5c5e\u7c92\u5b50\uff08\u591a\u304f\u306e\u5834\u5408\u3001\u91d1\u307e\u305f\u306f\u30bf\u30f3\u30b0\u30b9\u30c6\u30f3\uff09\u3092\u5f3e\u9053\u88c5\u7f6e\uff08\u3059\u306a\u308f\u3061\u3001&#8221;\u907a\u4f1d\u5b50\u9283&#8221;\uff09\u3092\u7528\u3044\u3066\u5bbf\u4e3b\u7d30\u80de\u306b\u9ad8\u901f\u3067\u5674\u5c04\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002\u907a\u4f1d\u5b50\u9283\u306b\u3088\u308b\u5fae\u7c92\u5b50\u306e\u5c0e\u5165\u306f\u3001\u57f9\u990a\u6db2\u4e2d\u306e\u5206\u88c2\u7d30\u80de\u304a\u3088\u3073\u975e\u5206\u88c2\u7d30\u80de\u306a\u3089\u3073\u306bin vivo\u306e\u7d30\u80de\u3078\u306e\u4e00\u904e\u6027\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u7528\u3044\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u3053\u308c\u306f\u907a\u4f1d\u5b50\u30ef\u30af\u30c1\u30f3\u63a5\u7a2e\u3084\u8fb2\u696d\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u304f\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\uff08Klein et al., 1992; Ye et al., 1990; Burkholder et al.,1993\uff09\u3002\u3053\u306e\u624b\u6cd5\u306f\u4fe1\u983c\u6027\u304c\u9ad8\u304f\u3001\u8fc5\u901f\u3067\u3042\u308b\u4e00\u65b9\u3001\u9ad8\u984d\u306a\u8a2d\u5099\u3092\u5fc5\u8981\u3068\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u306b\u7269\u7406\u7684\u306a\u30c0\u30e1\u30fc\u30b8\u3092\u4e0e\u3048\u3001\u7d30\u80de\u6b7b\u4ea1\u7387\u304c\u9ad8\u3044\u3053\u3068\u304b\u3089\u591a\u304f\u306e\u7d30\u80de\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002<\/p>\n<p><strong>\u76f4\u63a5\u30de\u30a4\u30af\u30ed\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3<\/strong>\u3067\u306f\u30011\u5ea6\u306b1\u7d30\u80de\u306b\u3064\u3044\u3066\u3001\u7d30\u91dd\u3092\u7528\u3044\u3066\u6838\u9178\u3092\u7d30\u80de\u8cea\u307e\u305f\u306f\u6838\u306b\u9001\u9054\u3057\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u3053\u306e\u65b9\u6cd5\u306e\u4f7f\u7528\u306f\u907a\u4f1d\u5b50\u3092\u5375\u6bcd\u7d30\u80de\u306b\u5c0e\u5165\u3057\u3066\u3001\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u52d5\u7269\u3092\u4f5c\u88fd\u3057\u305f\u308a\u3001\u4eba\u5de5\u67d3\u8272\u4f53\u3092\u9001\u9054\u3059\u308b\u3088\u3046\u306aex vivo\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u9650\u5b9a\u3055\u308c\u307e\u3059\uff08Cappechi, 1980; Cappechi, 1989; Telenius et al., 1999\uff09\u3002\u76f4\u63a5\u30de\u30a4\u30af\u30ed\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u5c0e\u5165\u52b9\u7387\u306f\u307b\u307c100%\u3067\u3059\u304c\u3001\u304b\u306a\u308a\u306e\u6280\u8853\u7684\u719f\u7df4\u304c\u5fc5\u8981\u3068\u3055\u308c\u3001\u6975\u3081\u3066\u52b4\u50cd\u96c6\u7d04\u7684\u3067\u3042\u308a\u3001\u9ad8\u983b\u5ea6\u3067\u7d30\u80de\u6b7b\u304c\u751f\u3058\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u3053\u306e\u65b9\u6cd5\u306f\u591a\u6570\u306e\u7d30\u80de\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u304c\u5fc5\u8981\u3068\u3055\u308c\u308b\u7814\u7a76\u306b\u306f\u9069\u3057\u307e\u305b\u3093\u3002<\/p>\n<p><strong>\u30ec\u30fc\u30b6\u30fc\u6cd5\u306b\u3088\u308b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3<\/strong>\u306f\u3001\u30d5\u30a9\u30c8\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3 \u3001\u30ec\u30fc\u30b6\u30fc\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3001\u3042\u308b\u3044\u306f\u30aa\u30d7\u30c8\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u3068\u3057\u3066\u3082\u77e5\u3089\u308c\u3001\u30ec\u30fc\u30b6\u30fc\u30d1\u30eb\u30b9\u3092\u7528\u3044\u3066\u4e00\u6642\u7684\u306b\u7d30\u80de\u819c\u3092\u900f\u904e\u3055\u305b\u308b\u65b9\u6cd5\u3067\u3059\uff08Shirahata et al., 2001; Schneckenburger et al., 2002\uff09\u3002\u30ec\u30fc\u30b6\u30fc\u306b\u3088\u308a\u7d30\u80de\u819c\u306b\u5b54\u304c\u751f\u3058\u308b\u3068\u3001\u57f9\u5730\u3068\u30b5\u30a4\u30c8\u30be\u30eb\u306e\u6d78\u900f\u5727\u5dee\u306b\u3088\u3063\u3066\u6838\u9178\u3084\u57f9\u5730\u4e2d\u306e\u4ed6\u306e\u6240\u671b\u306e\u7269\u8cea\uff08\u30a4\u30aa\u30f3\u3001\u4f4e\u5206\u5b50\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u534a\u5c0e\u4f53\u30ca\u30ce\u7d50\u6676\u306a\u3069\uff09\u306e\u7d30\u80de\u3078\u306e\u4fb5\u5165\u304c\u4fc3\u9032\u3055\u308c\u307e\u3059\u3002\u30ec\u30fc\u30b6\u30fc\u6cd5\u306b\u3088\u308b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u5229\u70b9\u306f\u3001\u9ad8\u3044\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3068\u7d30\u80de\u306e\u3042\u3089\u3086\u308b\u4f4d\u7f6e\u306b\u5b54\u3092\u5f62\u6210\u3067\u304d\u308b\u80fd\u529b\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u3053\u306e\u65b9\u6cd5\u306b\u306f\u9ad8\u4fa1\u306a\u30ec\u30fc\u30b6\u30fc\u9855\u5fae\u93e1\u30b7\u30b9\u30c6\u30e0\u3068\u7d30\u80de\u304c\u57fa\u8cea\u306b\u4ed8\u7740\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002<\/p>\n<p>\u4e0a\u8ff0\u306e\u65b9\u6cd5\u306b\u52a0\u3048\u3066\u3001\u4ed6\u306e\u7269\u7406\u7684\u5c0e\u5165\u6cd5\u3068\u3057\u3066\u3001\u52d5\u5727\u3001\u8d85\u97f3\u6ce2\u3001\u307e\u305f\u306f\u78c1\u5834\u3092\u7528\u3044\u3066\u3001\u88f8\u306e\u6838\u9178\u307e\u305f\u306f\u6838\u9178\u2015\u5fae\u7c92\u5b50\u8907\u5408\u4f53\u3092\u5bbf\u4e3b\u7d30\u80de\u306b\u9001\u9054\u3059\u308b\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>Gibco\u7d30\u80de\u57f9\u990a\u57fa\u790e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h2>\n<p>\u7d30\u80de\u57f9\u990a\u306b\u95a2\u3059\u308b\u57fa\u790e\u60c5\u5831\u3092\u89e3\u8aac\u3057\u305f\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\uff08\u65e5\u672c\u8a9e\u7248\u3001\u7d04100\u30da\u30fc\u30b8\uff09\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<br \/>\n<a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/gibco-cell-culture-basics-handbook-form.html?CID=bid_clb_tfx_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cc13_Social_LAB\" target=\"_blank\" rel=\"noopener 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