{"id":594,"date":"2017-02-07T00:00:00","date_gmt":"2017-02-07T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/about-clariom-d\/"},"modified":"2021-12-14T14:31:45","modified_gmt":"2021-12-14T05:31:45","slug":"about-clariom-d","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/about-clariom-d\/","title":{"rendered":"\u6b21\u4e16\u4ee3\u578b\u8d85\u9ad8\u5bc6\u5ea6\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4 Clariom D \u306e\u5727\u5012\u7684\u306a\u89e3\u6790\u6027\u80fd\u3068\u306f\uff1f"},"content":{"rendered":"

\u306f\u3058\u3081\u306b<\/h2>\n

\u5f0a\u793e\u306e\u6b21\u4e16\u4ee3\u578b\u767a\u73fe\u89e3\u6790\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u300cClariom D<\/a>\u300d\u306f\u3001\u7dcf\u30d7\u30ed\u30fc\u30d6\u6570600\u4e07\u4ee5\u4e0a\u3092\u8a87\u308b\u8d85\u9ad8\u5bc6\u5ea6\u306e\u591a\u6a5f\u80fd\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3067\u3042\u308a\u3001\u5f0a\u793e\u306e\u767a\u73fe\u89e3\u6790\u88fd\u54c1\u306e\u4e2d\u3067\u307e\u3055\u306b\u30d5\u30e9\u30c3\u30b0\u30b7\u30c3\u30d7\u3068\u547c\u3076\u306b\u3075\u3055\u308f\u3057\u3044\u30e2\u30c7\u30eb\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002Clariom D<\/a>\u306f\u5f93\u6765\u306e\u767a\u73fe\u89e3\u6790\u306b\u52a0\u3048\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u89e3\u6790\u3084long non-coding RNA (lncRNA) \u89e3\u6790\u306a\u3069\u3001\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30c8\u30fc\u30e0\u30ec\u30d9\u30eb\u3067\u306e\u89e3\u6790\u306b\u3082\u5a01\u529b\u3092\u767a\u63ee\u3057\u307e\u3059\u3002<\/strong>\u3053\u3053\u3067\u306fClariom D<\/a>\u306e\u5727\u5012\u7684\u306a\u89e3\u6790\u6027\u80fd\u306b\u3064\u3044\u3066\u307e\u3068\u3081\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n

\u5727\u5012\u7684\u306a\u30d7\u30ed\u30fc\u30d6\u6570\u3067\u8ee2\u5199\u9818\u57df\u3092\u30ab\u30d0\u30fc<\/h2>\n

Clariom D<\/a>\u306e\u6027\u80fd\u3092\u8a9e\u308b\u4e0a\u3067\u6b20\u304b\u3059\u3053\u3068\u306e\u3067\u304d\u306a\u3044\u306e\u304c\u3001\u305d\u306e\u30d7\u30ed\u30fc\u30d6\u6570\u3067\u3057\u3087\u3046\u3002600\u4e07\u3092\u8d85\u3048\u308b\u30d7\u30ed\u30fc\u30d6\u304c\u907a\u4f1d\u5b50\u767a\u73fe\u9818\u57df\u3092\u5e83\u7bc4\u56f2\u306b\u308f\u305f\u308a\u30ab\u30d0\u30fc\u3057\u307e\u3059\u3002<\/strong>\u4e0b\u56f3\u306fClariom D<\/a>\u306b\u642d\u8f09\u3055\u308c\u305f\u5404\u30d7\u30ed\u30fc\u30d6\u304c\u907a\u4f1d\u5b50\u306e\u3069\u306e\u914d\u5217\u306b\u5bfe\u5fdc\u3057\u3066\u3044\u308b\u304b\u3092\u793a\u3057\u305f\u3082\u306e\u3067\u3059\u3002<\/p>\n

\"about-clariom-d-fig1\"<\/a>

\u56f31 COLCA2 (Colorectal Cancer Associated 2) \u907a\u4f1d\u5b50\u306e\u30a8\u30af\u30bd\u30f3\u30fb\u30a4\u30f3\u30c8\u30ed\u30f3\u69cb\u9020\u3068\u30d7\u30ed\u30fc\u30d6\u306e\u4f4d\u7f6e\u95a2\u4fc2\u306e\u6a21\u5f0f\u56f3<\/p><\/div>\n

\u4e0a\u56f3\u306fCOLCA2 (Colorectal Cancer Associated 2) \u907a\u4f1d\u5b50\u306e\u30a8\u30af\u30bd\u30f3\u30fb\u30a4\u30f3\u30c8\u30ed\u30f3\u69cb\u9020\u3068\u30d7\u30ed\u30fc\u30d6\u306e\u4f4d\u7f6e\u95a2\u4fc2\u3092\u6a21\u5f0f\u7684\u306b\u793a\u3057\u305f\u3082\u306e\u3067\u3059\u3002\u3053\u306e\u56f3\u306e\u8d64\u3044\u67a0\u7dda\u3067\u56f2\u307e\u308c\u305f\u90e8\u5206\u304c\u30a8\u30af\u30bd\u30f3\u90e8\u5206\u306b\u5bfe\u5fdc\u3057\u3066\u3044\u308b\u30d7\u30ed\u30fc\u30d6\u7fa4\u3067\u3059\u3002\u3053\u306e\u56f3\u304b\u3089\u5206\u304b\u308b\u3088\u3046\u306b\u3001Clariom D<\/a>\u306e\u30d7\u30ed\u30fc\u30d6\u306f\u907a\u4f1d\u5b50\u5168\u4f53\u306b\u308f\u305f\u3063\u3066\u307b\u307c\u5168\u3066\u306e\u30a8\u30af\u30bd\u30f3\u306b\u5bfe\u5fdc\u3059\u308b\u3088\u3046\u306b\u8a2d\u8a08\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/strong>\u307e\u305f\u4e0a\u56f3\u306e\u30b0\u30ec\u30fc\u306e\u7dda\u3067\u56f2\u307e\u308c\u305f\u90e8\u5206\u306f\u3001\u30a8\u30af\u30bd\u30f3\u3068\u30a8\u30af\u30bd\u30f3\u306e\u63a5\u5408\u90e8\u3001\u3044\u308f\u3086\u308b\u30a8\u30af\u30bd\u30f3\u30b8\u30e3\u30f3\u30af\u30b7\u30e7\u30f3\u90e8\u306b\u8a2d\u8a08\u3055\u308c\u305f\u30d7\u30ed\u30fc\u30d6\u3067\u3059\u3002\u3053\u306e\u30d7\u30ed\u30fc\u30d6\u306b\u3088\u308a\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u306e\u89e3\u6790\u7cbe\u5ea6\u304c\u4e0a\u304c\u308a\u307e\u3059\u3002<\/p>\n

\u3053\u306e\u3088\u3046\u306bClariom D<\/a>\u3067\u306f\u4e00\u3064\u306e\u907a\u4f1d\u5b50\u306b\u5bfe\u3057\u3066\u6570\u5341\u3082\u306e\u30d7\u30ed\u30fc\u30d6\u304c\u8a2d\u8a08\u3055\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u5358\u306a\u308b\u907a\u4f1d\u5b50\u30ec\u30d9\u30eb\u306e\u767a\u73fe\u89e3\u6790\u3060\u3051\u306b\u3068\u3069\u307e\u3089\u306a\u3044\u3001\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30c8\u30fc\u30e0\u30ec\u30d9\u30eb\u306b\u3088\u308b\u89e3\u6790\u304c\u53ef\u80fd\u3068\u306a\u3063\u3066\u3044\u308b\u306e\u3067\u3059\u3002<\/strong><\/p>\n

\u3061\u306a\u307f\u306b\u3001\u5f0a\u793e\u4ee5\u5916\u306e\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3067\u306f\u591a\u304f\u306e\u5834\u5408\u30011\u907a\u4f1d\u5b50\u3092\u305f\u3063\u305f\u4e00\u3064\u306e\u30d7\u30ed\u30fc\u30d6\u3067\u89e3\u6790\u3057\u3066\u3044\u307e\u3059\u3002\u305f\u3063\u305f\u4e00\u3064\u306e\u30d7\u30ed\u30fc\u30d6\u3060\u3051\u30671\u907a\u4f1d\u5b50\u3092\u898b\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u306f\u3001\u4eee\u306b\u305d\u306e\u30d7\u30ed\u30fc\u30d6\u304c\u4f55\u3089\u304b\u306e\u7406\u7531\u3067\u6a5f\u80fd\u3057\u306a\u304b\u3063\u305f\u5834\u5408\u306f\u3001\u305d\u306e\u907a\u4f1d\u5b50\u306e\u60c5\u5831\u304c\u5168\u304f\u5f97\u3089\u308c\u306a\u3044\u3053\u3068\u3092\u610f\u5473\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305f1\u3064\u306e\u30d7\u30ed\u30fc\u30d6\u30c7\u30fc\u30bf\u3067\u306f\u7d71\u8a08\u7684\u306a\u51e6\u7406\u304c\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3001\u30c7\u30fc\u30bf\u306e\u4fe1\u983c\u6027\u306b\u7591\u554f\u304c\u6b8b\u308a\u307e\u3059\u3002<\/p>\n

\"about-clariom-d-fig2\"<\/a>

\u56f32 \u5f0a\u793e\u88fd\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u30d7\u30ed\u30fc\u30d6\u3068\u4ed6\u793e\u88fd\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u30d7\u30ed\u30fc\u30d6\u306e\u9055\u3044<\/p><\/div>\n

\u3053\u306e\u3088\u3046\u306a\u30c7\u30e1\u30ea\u30c3\u30c8\u306b\u3082\u304b\u304b\u308f\u3089\u305a\u5358\u4e00\u30d7\u30ed\u30fc\u30d6\u3067\u89e3\u6790\u305b\u3056\u308b\u3092\u5f97\u306a\u3044\u306e\u306f\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u642d\u8f09\u3067\u304d\u308b\u30d7\u30ed\u30fc\u30d6\u6570\u306b\u9650\u754c\u304c\u3042\u308b\u305f\u3081<\/strong>\u3067\u3059\u3002\u4e00\u822c\u7684\u306a\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u642d\u8f09\u3067\u304d\u308b\u30d7\u30ed\u30fc\u30d6\u6570\u306f\u6570\u4e07\u500b\u7a0b\u5ea6\u3057\u304b\u306a\u3044\u305f\u3081\u30011\u907a\u4f1d\u5b50\u306b\u8907\u6570\u306e\u30d7\u30ed\u30fc\u30d6\u3092\u5272\u308a\u5f53\u3066\u3066\u3057\u307e\u3046\u3068\u89e3\u6790\u3067\u304d\u308b\u907a\u4f1d\u5b50\u6570\u304c\u6e1b\u3063\u3066\u3057\u307e\u3046\u306e\u3067\u3059\u3002<\/p>\n

\"about-clariom-d-fig3\"<\/a>

\u56f33 \u5f0a\u793e\u88fd\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3068\u4e00\u822c\u7684\u306a\u767a\u73fe\u30a2\u30ec\u30a4\u3068\u306e\u30d7\u30ed\u30fc\u30d6\u6570\u306e\u9055\u3044<\/p><\/div>\n

\u5f0a\u793e\u306e\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306f\u534a\u5c0e\u88fd\u9020\u6280\u8853\u3092\u5fdc\u7528\u3057\u3066\u304a\u308a\u3001\u30d7\u30ed\u30fc\u30d6\u6570\u304c600\u4e07\u3092\u8d85\u3048\u308b\u3088\u3046\u306a\u8d85\u9ad8\u5bc6\u5ea6\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u4f5c\u6210\u3067\u304d\u307e\u3059\u3002<\/strong>\u3053\u308c\u306b\u3088\u308a\u3001Clariom D<\/a>\u306e\u3088\u3046\u306b1\u907a\u4f1d\u5b50\u306b\u6570\u5341\u3082\u306e\u30d7\u30ed\u30fc\u30d6\u3092\u8a2d\u8a08\u3057\u3066\u3082\u306a\u304a\u6570\u4e07\u306b\u3082\u53ca\u3076\u907a\u4f1d\u5b50\u306e\u89e3\u6790\u304c\u53ef\u80fd\u306a\u306e\u3067\u3059\u3002<\/p>\n

Clariom D\u3067\u89e3\u6790\u53ef\u80fd\u306a\u907a\u4f1d\u5b50<\/h2>\n

Clariom D<\/a>\u306e\u30d2\u30c8\u3001\u30de\u30a6\u30b9\u3001\u30e9\u30c3\u30c8\u305d\u308c\u305e\u308c\u306e\u88fd\u54c1\u3067\u89e3\u6790\u3067\u304d\u308b\u907a\u4f1d\u5b50\u6570\u306a\u3069\u306f\u4e0b\u8a18\u306e\u901a\u308a\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u88681 Clariom D\u3067\u89e3\u6790\u3067\u304d\u308b\u907a\u4f1d\u5b50\u6570<\/caption>\n
Content summary<\/strong><\/td>\nHuman<\/strong><\/td>\nMouse<\/strong><\/td>\nRat<\/strong><\/td>\n<\/tr>\n
Genes\u203b<\/sup><\/td>\n>134,700<\/td>\n>66,100<\/td>\n>68,900<\/td>\n<\/tr>\n
Transcripts\u203b<\/sup><\/td>\n>542,500<\/td>\n>214,900<\/td>\n>495,200<\/td>\n<\/tr>\n
Exons\u203b<\/sup><\/td>\n>948,300<\/td>\n>498,500<\/td>\n>320,400<\/td>\n<\/tr>\n
Exon-exon splice junctions\u203b<\/sup><\/td>\n>484,900<\/td>\n>282,500<\/td>\n>293,700<\/td>\n<\/tr>\n
Total probes\u203b<\/sup><\/td>\n>6,765,500<\/td>\n>6,022,300<\/td>\n>5,946,400<\/td>\n<\/tr>\n
Probes targeting exons\u203b<\/sup><\/td>\n>4,781,200<\/td>\n>4,895,600<\/td>\n>4,780,700<\/td>\n<\/tr>\n
Probes targeting exon-exon splice junctions\u203b<\/sup><\/td>\n>1,984,300<\/td>\n>1,126,700<\/td>\n>1,165,700<\/td>\n<\/tr>\n
Probe length(bases)<\/td>\n25<\/td>\n25<\/td>\n25<\/td>\n<\/tr>\n
Probe feature size<\/td>\n5\u03bcm<\/td>\n5\u03bcm<\/td>\n5\u03bcm<\/td>\n<\/tr>\n
Background probes<\/td>\nAntigenomic Set<\/td>\nAntigenomic Set<\/td>\nAntigenomic Set<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b2016\u5e744\u6708\u6642\u70b9\u3067\u306e\u4ee3\u8868\u7684\u306a\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u306e\u6570\u3067\u3059\u300210\u306e\u4f4d\u4ee5\u4e0b\u3092\u5207\u308a\u6368\u3066\u3066\u8868\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/span>
\n\u4f8b\u3048\u3070\u30d2\u30c8\u7528\u306eClariom D<\/a>\u306f134,700\u4ee5\u4e0a\u306e\u907a\u4f1d\u5b50\u3092\u89e3\u6790\u3067\u304d\u3001\u3053\u306e\u4e2d\u306b\u306f542,500\u4ee5\u4e0a\u306e\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u30d0\u30ea\u30a2\u30f3\u30c8\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u30d7\u30ed\u30fc\u30d6\u306e\u8a2d\u8a08\u306b\u3042\u305f\u3063\u3066\u306f15\u7a2e\u985e\u306b\u3082\u53ca\u3076\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3092\u5229\u7528\u3057\u3066\u304a\u308a\u3001\u73fe\u5728\u307e\u3067\u306b\u77e5\u3089\u308c\u3066\u3044\u308b\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u30d0\u30ea\u30a2\u30f3\u30c8\u3092\u7db2\u7f85\u3057\u3066\u3044\u307e\u3059\u3002
\n\"about-clariom-d-fig4\"<\/a>
\n\u307e\u305f\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u907a\u4f1d\u5b50\u306e\u307f\u306a\u3089\u305a\u3001\u30ce\u30f3\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0 (non-coding) \u907a\u4f1d\u5b50\u306b\u95a2\u3059\u308b\u30d7\u30ed\u30fc\u30d6\u3082\u8c4a\u5bcc\u306b\u542b\u307e\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u63a2\u7d22\u306a\u3069\u306e\u305f\u3081\u306e\u60c5\u5831\u3092\u9003\u3057\u307e\u305b\u3093\u3002<\/p>\n

RNA-Seq\u3092\u51cc\u3050\u7cbe\u5ea6\u3067\u4f4e\u767a\u73fe\u907a\u4f1d\u5b50\u3082\u3057\u3063\u304b\u308a\u89e3\u6790<\/h2>\n

\u8fd1\u5e74\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\uff08NGS\uff09\u306e\u6027\u80fd\u304c\u5411\u4e0a\u3059\u308b\u306b\u3064\u308c\u3066\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u89e3\u6790\u3092NGS\u3067\u304a\u3053\u306a\u3046\u3001\u3044\u308f\u3086\u308bRNA-Seq\u306e\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u304c\u5e83\u304f\u7528\u3044\u3089\u308c\u308b\u3088\u3046\u306b\u306a\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u767a\u73fe\u91cf\u3060\u3051\u3067\u306a\u304f\u914d\u5217\u60c5\u5831\u3082\u540c\u6642\u306b\u5f97\u3089\u308c\u308bRNA-Seq\u306f\u3001\u307e\u3055\u306b\u6b21\u4e16\u4ee3\u306e\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3068\u3057\u3066\u69d8\u3005\u306a\u5834\u9762\u3067\u6d3b\u7528\u3055\u308c\u59cb\u3081\u3066\u3044\u307e\u3059\u3002<\/strong>\u4e00\u65b9\u3067RNA-Seq\u3092\u4f7f\u3044\u3053\u306a\u3059\u305f\u3081\u306b\u306f\u3001\u305d\u306e\u7279\u5fb4\u3092\u4e88\u3081\u3057\u3063\u304b\u308a\u3068\u628a\u63e1\u3057\u3066\u304a\u304f\u3053\u3068\u304c\u809d\u5fc3\u3067\u3059\u3002\u5834\u5408\u306b\u3088\u3063\u3066\u306f\u601d\u3063\u3066\u3044\u305f\u3088\u3046\u306a\u6027\u80fd\u304c\u51fa\u305a\u306b\u3001\u6642\u9593\u3068\u30b3\u30b9\u30c8\u3092\u7121\u99c4\u306b\u3057\u3066\u3057\u307e\u3044\u304b\u306d\u307e\u305b\u3093\u3002<\/p>\n

RNA-Seq\u306e\u6027\u80fd\u3092\u7aef\u7684\u306b\u8868\u3057\u305f\u3082\u306e\u3068\u3057\u3066\u3001\u4e0b\u56f3\u306e\u3088\u3046\u306a\u30c7\u30fc\u30bf\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\"about-clariom-d-fig5\"<\/a>

\u56f35 RNA-Seq\u3068\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u3088\u308b\u767a\u73fe\u89e3\u6790\u306e\u6bd4\u8f03\u3002\u203bXu, W., et al. Human transcriptome array for high throughput clinical studies. Proc Natl Acad Sci USA 108(9):3707\u20133712 (2011).<\/span><\/p><\/div>\n

\u4e0a\u56f3\u306fRNA-Seq\u3068\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u3088\u308b\u767a\u73fe\u89e3\u6790\u3092\u6bd4\u8f03\u3057\u305f\u3082\u306e\u3067\u3059\u3002\u56f3\u306e\u6a2a\u8ef8\u306f\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u3092\u3001\u7e26\u8ef8\u306f\u6e2c\u5b9a\u5024\u306e\u3070\u3089\u3064\u304d\u306e\u5927\u304d\u3055\u3092\u8868\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u56f3\u306e\u5de6\u4e0a\u30011500\u4e07\u3068\u8d64\u5b57\u3067\u66f8\u304b\u308c\u3066\u3044\u308b\u30b0\u30e9\u30d5\u306b\u6ce8\u76ee\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u306e\u8d64\u5b57\u306fRNA-Seq\u306e\u30ea\u30fc\u30c9\u6570\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306f\u4f4e\u767a\u73fe\u306e\u907a\u4f1d\u5b50\u3067\u3082\u6e2c\u5b9a\u5024\u306e\u3070\u3089\u3064\u304d\u304c\u5c0f\u3055\u3044\u306e\u306b\u5bfe\u3057\u3001RNA-Seq\u3067\u306f\u767a\u73fe\u91cf\u304c\u4f4e\u304f\u306a\u308b\u306b\u3064\u308c\u3066\u6e2c\u5b9a\u306e\u3070\u3089\u3064\u304d\u304c\u3069\u3093\u3069\u3093\u5927\u304d\u304f\u306a\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u30ea\u30fc\u30c9\u6570\u304c\u5c11\u306a\u3044\u6761\u4ef6\u3067\u306eRNA-Seq\u3067\u306f\u4f4e\u767a\u73fe\u306e\u907a\u4f1d\u5b50\u3092\u7cbe\u5ea6\u3088\u304f\u89e3\u6790\u3067\u304d\u307e\u305b\u3093\u3002\u3082\u3057\u3082RNA-Seq\u3067\u4f4e\u767a\u73fe\u907a\u4f1d\u5b50\u3092\u9ad8\u7cbe\u5ea6\u3067\u89e3\u6790\u3057\u305f\u3051\u308c\u3070\u3001\u30ea\u30fc\u30c9\u6570\u3092\u5897\u3084\u3057\u3066\u3044\u304f\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/strong>\u56f3\u306e\u53f3\u4e0b\u306e\u3088\u3046\u306b4.8\u5104\u30ea\u30fc\u30c9\u307e\u3067\u30ea\u30fc\u30c9\u6570\u3092\u5897\u3084\u3059\u3053\u3068\u306b\u3088\u308a\u3001\u3088\u3046\u3084\u304f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3068\u540c\u7a0b\u5ea6\u306e\u89e3\u6790\u6027\u80fd\u3092\u6301\u3064\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002<\/p>\n

\u4e00\u822c\u7684\u306b\u304a\u3053\u306a\u308f\u308c\u3066\u3044\u308bRNA-Seq\u3067\u306f\u30ea\u30fc\u30c9\u6570\u306b\u3057\u30664000\u4e07 (40 million) \u7a0b\u5ea6\u3067\u89e3\u6790\u3059\u308b\u3053\u3068\u304c\u591a\u3044\u3088\u3046\u3067\u3059\u3002\u4e0a\u8ff0\u3057\u305f\u3068\u304a\u308a\u3001\u4f4e\u767a\u73fe\u306e\u907a\u4f1d\u5b50\u307e\u3067\u7cbe\u5ea6\u3088\u304f\u6e2c\u5b9a\u3059\u308b\u305f\u3081\u306b\u306f\u5c11\u306a\u304f\u3068\u3082\u3053\u306e10\u500d\u4ee5\u4e0a\u306e\u30ea\u30fc\u30c9\u6570\u304c\u5fc5\u8981\u3067\u3059\u3002\u305d\u308c\u306f\u3059\u306a\u308f\u3061\u3001\u4e00\u822c\u7684\u306aRNA-Seq\u306b\u6bd4\u3079\u306610\u500d\u306e\u30b3\u30b9\u30c8\u304c\u304b\u304b\u308b\u3053\u3068\u3092\u610f\u5473\u3057\u3066\u3044\u307e\u3059\u3002RNA-Seq\u3067\u3057\u304b\u5f97\u3089\u308c\u306a\u3044\u60c5\u5831\u304c\u5fc5\u8981\u3067\u3042\u308c\u3070\u3053\u306e\u30b3\u30b9\u30c8\u306f\u5fc5\u8981\u7d4c\u8cbb\u3068\u3057\u3066\u5272\u308a\u5207\u308c\u307e\u3059\u304c\u3001\u3082\u3057\u3082\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u4e0a\u306b\u3042\u308b\u65e2\u5b58\u907a\u4f1d\u5b50\u306e\u89e3\u6790\u304c\u4e3b\u306a\u76ee\u7684\u3067\u3042\u308b\u306e\u306a\u3089\u3070\u3001Clariom D<\/a>\u3092\u7528\u3044\u305f\u65b9\u304c\u306f\u308b\u304b\u306b\u9ad8\u7cbe\u5ea6\u304b\u3064\u4f4e\u30b3\u30b9\u30c8\u3067\u89e3\u6790\u3067\u304d\u308b\u306e\u3067\u3059\u3002<\/strong><\/p>\n

\u907a\u4f1d\u5b50\u30ec\u30d9\u30eb\u3092\u8d85\u3048\u305f\u3001\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30c8\u30fc\u30e0\u30ec\u30d9\u30eb\u89e3\u6790\u3092\u5b9f\u73fe<\/h2>\n

Clariom D<\/a>\u306f\u5f93\u6765\u306e\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3067\u4e00\u822c\u7684\u306b\u304a\u3053\u306a\u308f\u308c\u3066\u3044\u308b\u907a\u4f1d\u5b50\u30ec\u30d9\u30eb\u306e\u767a\u73fe\u89e3\u6790\u306e\u307b\u304b\u306b\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u306b\u3088\u308b\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u30d0\u30ea\u30a2\u30f3\u30c8\u89e3\u6790\u3084\u3001long non-coding RNA (lncRNA) \u89e3\u6790\u306a\u3069\u3001\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30c8\u30fc\u30e0\u30ec\u30d9\u30eb\u3067\u306e\u89e3\u6790\u3092\u304a\u3053\u306a\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u6b21\u4e16\u4ee3\u578b\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3067\u3059\u3002<\/strong>\u3053\u3053\u3067\u306f\u3001Clariom D<\/a>\u306b\u3088\u308b\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30c8\u30fc\u30e0\u30ec\u30d9\u30eb\u89e3\u6790\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u306b\u3088\u308b\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u30d0\u30ea\u30a2\u30f3\u30c8\u89e3\u6790<\/h3>\n

Clariom D<\/a>\u306e\u8d85\u9ad8\u5bc6\u5ea6\u30d7\u30ed\u30fc\u30d6\u306f\u907a\u4f1d\u5b50\u9818\u57df\u3092\u304f\u307e\u306a\u304f\u30ab\u30d0\u30fc\u3059\u308b\u3060\u3051\u3067\u306a\u304f\u3001\u30a8\u30af\u30bd\u30f3\u30b8\u30e3\u30f3\u30af\u30b7\u30e7\u30f3\u90e8\u306b\u3082\u8a2d\u8a08\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u30a8\u30af\u30bd\u30f3\u30ec\u30d9\u30eb\u3067\u306e\u7cbe\u5bc6\u306a\u767a\u73fe\u89e3\u6790\u304c\u53ef\u80fd\u3068\u306a\u308a\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u306b\u3088\u308b\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u30d0\u30ea\u30a2\u30f3\u30c8\u89e3\u6790\u3092\u304a\u3053\u306a\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/strong><\/p>\n

\u4f8b\u3048\u3070\u5192\u982d\u306eCOLCA2\u907a\u4f1d\u5b50\u306f\u3001\u6700\u65b0\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306e\u77e5\u898b\u306b\u3088\u308a10\u7a2e\u985e\u306e\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u30d0\u30ea\u30a2\u30f3\u30c8\u304c\u5b58\u5728\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002
\n\"about-clariom-d-fig6\"<\/a>
\nClariom D<\/a>\u306f\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u306b\u52a0\u3048\u3001\u5404\u30a8\u30af\u30bd\u30f3\u3054\u3068\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u3082\u540c\u6642\u306b\u89e3\u6790\u3057\u307e\u3059\u3002\u5c02\u7528\u306e\u89e3\u6790\u30bd\u30d5\u30c8\u3092\u7528\u3044\u308c\u3070\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u542b\u307e\u308c\u308b\u30d0\u30ea\u30a2\u30f3\u30c8\u306e\u3046\u3061\u3001\u3069\u306e\u30bf\u30a4\u30d7\u304c\u3082\u3063\u3068\u3082\u591a\u304f\u767a\u73fe\u3057\u3066\u3044\u308b\u304b\u3092\u4e88\u6e2c\u3067\u304d\u307e\u3059\u3002<\/p>\n

long non-coding RNA (lncRNA) \u89e3\u6790<\/h3>\n

Clariom D<\/a>\u306b\u306flong non-coding RNA (lncRNA) \u3092\u89e3\u6790\u3059\u308b\u305f\u3081\u306e\u30d7\u30ed\u30fc\u30d6\u304c\u6570\u591a\u304f\u642d\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4e0b\u8a18\u306e\u8868\u306fClariom D<\/a>\u3092\u8a2d\u8a08\u3059\u308b\u969b\u306b\u7528\u3044\u3089\u308c\u305f\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306e\u4e00\u89a7\u3068\u3001\u63a1\u7528\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6570\u3092\u8868\u3057\u305f\u3082\u306e\u3067\u3059\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u88682 Clariom D\u3092\u8a2d\u8a08\u3059\u308b\u969b\u306b\u7528\u3044\u3089\u308c\u305f\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u304a\u3088\u3073Clariom D\u306b\u63a1\u7528\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6570<\/caption>\n
Data sources<\/strong>\u00a0<\/strong><\/td>\nGenes<\/strong><\/td>\n<\/tr>\n
Human<\/strong><\/td>\nMouse<\/strong><\/td>\nRat<\/strong><\/td>\n<\/tr>\n
Ensembl<\/td>\n>57,500<\/td>\n>57,500<\/td>\n>25,300<\/td>\n<\/tr>\n
VEGA<\/td>\n>48,500<\/td>\n>23,200<\/td>\n\u2013<\/td>\n<\/tr>\n
NONCODE<\/td>\n>55,900<\/td>\n>42,000<\/td>\n>500<\/td>\n<\/tr>\n
lncRNAWiki<\/td>\n>50,000<\/td>\n\u2013<\/td>\n\u2013<\/td>\n<\/tr>\n
UCSC Genes<\/td>\n>43,800<\/td>\n>26,300<\/td>\n\u2013<\/td>\n<\/tr>\n
AceView<\/td>\n>41,100<\/td>\n\u2013<\/td>\n>30,900<\/td>\n<\/tr>\n
miTranscriptome<\/td>\n>34,500<\/td>\n\u2013<\/td>\n\u2013<\/td>\n<\/tr>\n
RefSeq<\/td>\n>25,600<\/td>\n>23,500<\/td>\n>16,900<\/td>\n<\/tr>\n
MGC<\/td>\n>17,200<\/td>\n>17,400<\/td>\n>6,400<\/td>\n<\/tr>\n
MGI<\/td>\n\u2013<\/td>\n>25,800<\/td>\n\u2013<\/td>\n<\/tr>\n
RGD<\/td>\n\u2013<\/td>\n\u2013<\/td>\n>30,300<\/td>\n<\/tr>\n
Consensus CDS<\/td>\n>18,500<\/td>\n\u2013<\/td>\n\u2013<\/td>\n<\/tr>\n
RNA Central<\/td>\n>17,200<\/td>\n\u2013<\/td>\n\u2013<\/td>\n<\/tr>\n
circBase<\/td>\n>12,200<\/td>\n\u2013<\/td>\n\u2013<\/td>\n<\/tr>\n
Human Body Map<\/td>\n>10,200<\/td>\n\u2013<\/td>\n\u2013<\/td>\n<\/tr>\n
lincRNAdb<\/td>\n>80<\/td>\n>60<\/td>\n>10<\/td>\n<\/tr>\n
Publication-specific gene sets<\/td>\n>3,000<\/td>\n>10,000<\/td>\n>10,290<\/td>\n<\/tr>\n
Non-overlapping orthologous mouse gene and transcript models<\/td>\n\u2013<\/td>\n\u2013<\/td>\n>21,500<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u3053\u306e\u8868\u304b\u3089\u660e\u3089\u304b\u306a\u3088\u3046\u306bClariom D<\/a>\u306b\u306fnon-coding RNA\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3092\u5143\u306b\u3057\u3066\u6570\u591a\u304f\u306e\u30d7\u30ed\u30fc\u30d6\u304c\u8a2d\u8a08\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u4eca\u307e\u3067\u898b\u904e\u3054\u3055\u308c\u3066\u304d\u305flncRNA\u306e\u767a\u73fe\u5909\u52d5\u3092\u78ba\u5b9f\u306b\u3068\u3089\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/strong><\/p>\n

\u4e00\u822c\u7684\u306blncRNA\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u306f\u304b\u306a\u308a\u4f4e\u3044\u305f\u3081\u3001\u5148\u8ff0\u3057\u305fRNA-Seq\u3067\u89e3\u6790\u3092\u3059\u308b\u305f\u3081\u306b\u306f\u76f8\u5f53\u6570\u306e\u30ea\u30fc\u30c9\u6570\u3092\u8aad\u3080\u5fc5\u8981\u304c\u3042\u308a\u3001\u3044\u308f\u3086\u308bDeep Sequencing\u304c\u5fc5\u8981\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u203b<\/sup>\u3002\u4e00\u65b9\u3067Deep Sequencing\u306f\u89e3\u6790\u30b3\u30b9\u30c8\u304c\u5897\u5927\u3059\u308b\u3070\u304b\u308a\u3067\u306a\u304f\u3001\u30c7\u30fc\u30bf\u89e3\u6790\u306e\u305f\u3081\u306e\u624b\u9593\u3084\u6642\u9593\u3082\u304b\u304b\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002Clariom D<\/a>\u306f\u5c02\u7528\u306e\u89e3\u6790\u30bd\u30d5\u30c8\u306b\u3088\u308a\u3069\u306a\u305f\u3067\u3082\u7c21\u5358\u306blncRNA\u306e\u767a\u73fe\u3092\u89e3\u6790\u3067\u304d\u308b\u305f\u3081\u3001lncRNA\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3057\u305f\u7814\u7a76\u306b\u5927\u304d\u306a\u5a01\u529b\u3092\u767a\u63ee\u3057\u307e\u3059\u3002<\/strong>
\n\u203bLiu, Y., et al. Evaluating the impact of sequencing depth on transcriptome profiling in human adipose. PLoS One 8(6):e66883 (2013).<\/span><\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u4eca\u56de\u306f\u6b21\u4e16\u4ee3\u578b\u8d85\u9ad8\u5bc6\u5ea6\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4Clariom D<\/a>\u306e\u7279\u5fb4\u3068\u6027\u80fd\u306b\u3064\u3044\u3066\u307f\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n

\u73fe\u5728\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3092\u7528\u3044\u305f\u767a\u73fe\u89e3\u6790\u306f\u6d41\u884c\u3068\u3044\u3063\u3066\u3044\u3044\u30ec\u30d9\u30eb\u3067\u5e83\u7bc4\u56f2\u306b\u308f\u305f\u3063\u3066\u5b9f\u65bd\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001RNA-Seq\u3067\u3067\u304d\u308b\u3053\u3068\u3001\u3067\u304d\u306a\u3044\u3053\u3068\u3092\u898b\u6975\u3081\u305f\u4e0a\u3067\u5b9f\u9a13\u3092\u3057\u3066\u3044\u308b\u4eba\u306f\u610f\u5916\u3068\u591a\u304f\u3042\u308a\u307e\u305b\u3093\u3002\u9650\u3089\u308c\u305f\u7814\u7a76\u4e88\u7b97\u306e\u4e2d\u3067\u771f\u306e\u610f\u5473\u3067\u306e\u4fa1\u5024\u3042\u308b\u30c7\u30fc\u30bf\u3092\u51fa\u3059\u305f\u3081\u306b\u306f\u3001\u6642\u6d41\u306b\u3068\u3089\u308f\u308c\u306a\u3044\u5408\u7406\u7684\u306a\u5224\u65ad\u304c\u6c42\u3081\u3089\u308c\u3066\u3044\u307e\u3059\u3002Clariom D<\/a>\u306f\u3001\u672a\u6765\u306b\u7e4b\u304c\u308b\u30c7\u30fc\u30bf\u3092\u6c42\u3081\u308b\u7814\u7a76\u8005\u306b\u3068\u3063\u3066\u6700\u5584\u306e\u7d50\u679c\u3092\u3082\u305f\u3089\u3059\u3053\u3068\u3067\u3057\u3087\u3046\u3002<\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u76ee\u7684\u304a\u3088\u3073\u305d\u306e\u624b\u7d9a\u304d\u4e0a\u3067\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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