{"id":60,"date":"2020-03-04T00:00:00","date_gmt":"2020-03-03T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/"},"modified":"2025-05-19T12:27:09","modified_gmt":"2025-05-19T03:27:09","slug":"protein-basic4","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/","title":{"rendered":"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de"},"content":{"rendered":"<p>\u7b2c4\u56de\u76ee\u306e\u4eca\u56de\u306f\u3001<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic3\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\">\u524d\u56de<\/a>\u306b\u5f15\u304d\u3064\u3065\u304d\u300c\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u300d\u3092\u30c6\u30fc\u30de\u3068\u3057\u3066\u3001\u5f0a\u793e\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u8a66\u85ac\u88fd\u54c1\u306e\u539f\u7406\u3084\u7279\u5fb4\u306b\u3064\u3044\u3066\u89e3\u8aac\u3057\u307e\u3059\uff01<\/p>\n<p>\u4eca\u56de\u306e\u30c6\u30fc\u30de\u306f\u300c\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u7a2e\u985e\u3068\u539f\u7406\u307e\u3068\u3081\u300d\u3068\u984c\u3057\u3066\u3001\u5f0a\u793e\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u8a66\u85ac\u88fd\u54c1\u306e\u7279\u5fb4\u3068\u76ee\u7684\u306b\u5408\u308f\u305b\u305f\u9078\u629e\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01&#8221;<\/p>\n<h2>BCA\u6cd5<\/h2>\n<p>BCA \u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306f\u30011985\u5e74\u306bPaul K. Smith\u3089\u306b\u3088\u3063\u3066\u7d39\u4ecb\u3055\u308c\u307e\u3057\u305f\u3002\u3053\u306eBCA\u6cd5\u306e\u5927\u304d\u306a\u7279\u9577\u306f\u3001\u305d\u308c\u307e\u3067Bradford\uff08Coomassie\uff09\u30d9\u30fc\u30b9\u306e\u65b9\u6cd5\u3067\u306f\u56f0\u96e3\u3067\u3042\u3063\u305f<strong>\u754c\u9762\u6d3b\u6027\u5264\u3092\u542b\u3080\u6eb6\u6db2\u306e\u6e2c\u5b9a\u306b\u3064\u3044\u3066\u3082\u53ef\u80fd\u306b\u306a\u3063\u305f<\/strong>\u3053\u3068\u3067\u3059\u3002<\/p>\n<p>\u5f0a\u793e\u306eBCA\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u7528\u30ad\u30c3\u30c8\u3067\u306f\u3001\u30aa\u30ea\u30b8\u30ca\u30eb\u306e<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/23225?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">BCA Protein Assay Kit<\/a>\u306b\u52a0\u3048\u3001\u53cd\u5fdc\u6642\u9593\u304c5\u5206\u306b\u77ed\u7e2e\u3055\u308c\u305f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A53227?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">Rapid Gold BCA Assay Kit<\/a>\u3001\u4f4e\u6fc3\u5ea6\u30bf\u30f3\u30d1\u30af\u8cea\u6eb6\u6db2\uff080.5-20 \u00b5g\/mL\uff09\u306e\u6e2c\u5b9a\u7528\u306b\u958b\u767a\u3055\u308c\u305f<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/23235?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Micro BCA Protein Assay Kit<\/a>\u3001\u5f93\u6765\u306f\u539f\u7406\u7684\u306b\u56f0\u96e3\u3067\u3042\u3063\u305f\u9084\u5143\u5264\u542b\u6709\u30b5\u30f3\u30d7\u30eb\u306b\u3064\u3044\u3066\u3082\u6e2c\u5b9a\u3067\u304d\u308b\u3088\u3046\u306b\u6539\u826f\u3055\u308c\u305f<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/23250?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">BCA Protein Assay Kit &#8211; Reducing Agent Compatible<\/a>\u3001\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u53cd\u5fdc\u7528\u306e<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/23252?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Microplate BCA Protein Assay Kit &#8211; Reducing Agent Compatible<\/a>\u306e\u30e9\u30a4\u30f3\u30a2\u30c3\u30d7\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h3>BCA\u6cd5\u306e\u5316\u5b66\u53cd\u5fdc<\/h3>\n<p>BCA\u6cd5\u306f\u3001\u30a2\u30eb\u30ab\u30ea\u6761\u4ef6\u4e0b\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3088\u3063\u30662\u4fa1\u306e\u9285\u30a4\u30aa\u30f3\uff08Cu2+\uff09\u304c1\u4fa1\u306e\u9285\u30a4\u30aa\u30f3\uff08Cu+\uff09\u306b\u9084\u5143\u3055\u308c\u308b\u539f\u7406\u3068\u30011\u4fa1\u306e\u9285\u30a4\u30aa\u30f3\u304c2\u5206\u5b50\u306e\u30d3\u30b7\u30f3\u30b3\u30cb\u30f3\u9178\uff08BCA: Bicinchoninic Acid\uff09\u3068\u914d\u4f4d\u7d50\u5408\u3057\u3066\u7d2b\u8272\u306b\u5448\u8272\u3059\u308b\u539f\u7406\u3092\u7d44\u307f\u5408\u308f\u305b\u305f\u65b9\u6cd5\u3067\u3059\uff08\u56f31\uff09\u3002<\/p>\n<div id=\"attachment_15889\" style=\"width: 356px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15889\" class=\"size-full wp-image-15889\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/03\/protein-basic4-01.png\" alt=\"BCA\u6cd5\u306e\u53cd\u5fdc\u30b9\u30ad\u30fc\u30e0\" width=\"346\" height=\"318\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/03\/protein-basic4-01.png 346w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/03\/protein-basic4-01-300x276.png 300w\" sizes=\"auto, (max-width: 346px) 100vw, 346px\" \/><p id=\"caption-attachment-15889\" class=\"wp-caption-text\">\u56f31 BCA\u6cd5\u306e\u53cd\u5fdc\u30b9\u30ad\u30fc\u30e0<\/p><\/div>\n<p>\u53cd\u5fdc\u306e\u6700\u521d\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u30682\u4fa1\u306e\u9285\u30a4\u30aa\u30f3\uff08Cu2+\uff09\u304c\u30ad\u30ec\u30fc\u30c8\u932f\u4f53\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u3053\u306e\u53cd\u5fdc\u306f\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u3068\u3057\u3066\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u3067\u306f\u9152\u77f3\u9178\u30ab\u30ea\u30a6\u30e0\u30ca\u30c8\u30ea\u30a6\u30e0\u3092\u542b\u3080\u30a2\u30eb\u30ab\u30ea\u6761\u4ef6\u4e0b\u30673\u30a2\u30df\u30ce\u9178\u4ee5\u4e0a\u306e\u30da\u30d7\u30c1\u30c9\u304c\u9285\u30a4\u30aa\u30f3\u3068\u30ad\u30ec\u30fc\u30c8\u932f\u4f53\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u3068\u3044\u3046\u547c\u3073\u540d\u306f\u3001\u3082\u3068\u3082\u3068\u6709\u6a5f\u5316\u5408\u7269\u306e\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\uff08NH2-CO-NH-CO-NH2\uff09\u304c\u9285\u30a4\u30aa\u30f3\u3068\u30ad\u30ec\u30fc\u30c8\u932f\u4f53\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u306b\u7531\u6765\u3057\u307e\u3059\u3002\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u5316\u5408\u7269\u306f\u5c3f\u7d20\u3068\u3068\u3082\u306b\u52a0\u71b1\u3059\u308b\u3068\u9285\u3068\u53cd\u5fdc\u3057\u3066\u9752\u8272\u306e\u56db\u5ea7\u914d\u4f4d\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u307e\u3059\uff08\u56f32\uff09\u3002\u30a2\u30df\u30ce\u9178\u306e\u6570\u304c1\u3064\u30842\u3064\u306e\u5834\u5408\u306b\u306f\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u306f\u8d77\u3053\u308a\u307e\u305b\u3093\u304c\u30013\u30a2\u30df\u30ce\u9178\u4ee5\u4e0a\u306e\u30da\u30d7\u30c1\u30c9\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u306f\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u304c\u8d77\u3053\u308a\u3001540 nm\u4ed8\u8fd1\u306b\u5438\u53ce\u306e\u3042\u308b\u8584\u3044\u9752\u8272\u304b\u3089\u7d2b\u8272\u306e\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u9285\u30a4\u30aa\u30f3\uff08Cu2+\uff09 1\u5206\u5b50\u3042\u305f\u308a4\u3064\u304b\u30896\u3064\u306e\u30da\u30d7\u30c1\u30c9\u7d50\u5408\u3068\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u307e\u3059\u3002<\/p>\n<div id=\"attachment_159\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-159\" class=\"wp-image-159 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-fig2.jpg\" alt=\"\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u306e\u30b9\u30ad\u30fc\u30e0\" width=\"500\" height=\"216\" \/><p id=\"caption-attachment-159\" class=\"wp-caption-text\">\u56f32 \u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u306e\u30b9\u30ad\u30fc\u30e0<\/p><\/div>\n<p>\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u5f8c\u306b2\u4fa1\u306e\u9285\u30a4\u30aa\u30f3\uff08Cu2+\uff09\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3088\u3063\u30661\u4fa1\u306e\u9285\u30a4\u30aa\u30f3\uff08Cu+\uff09\u306b\u9084\u5143\u3055\u308c\u307e\u3059\u3002\u3053\u306eCu+ \u306f2\u5206\u5b50\u306eBCA\u8a66\u85ac\u3068\u7d2b\u8272\u306e\u932f\u4f53\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u3067\u5f62\u6210\u3055\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u9285\u30a4\u30aa\u30f3\u306e\u8907\u5408\u4f53\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30da\u30d7\u30c1\u30c9\u7d50\u5408\u306e\u6570\u306b\u6bd4\u4f8b\u3057\u307e\u3059\u3002\u9285\u30a4\u30aa\u30f3\u306e\u9084\u5143\u53cd\u5fdc\u306f\u4e3b\u306b\u30da\u30d7\u30c1\u30c9\u7d50\u5408\u3068\u306e\u53cd\u5fdc\u3067\u884c\u308f\u308c\u307e\u3059\uff08\u305f\u3060\u3057\u30b7\u30b9\u30c6\u30a4\u30f3\u307e\u305f\u306f\u30b7\u30b9\u30c1\u30f3\u6b8b\u57fa\u3001\u30c1\u30ed\u30b7\u30f3\u6b8b\u57fa\u3001\u30c8\u30ea\u30d7\u30c8\u30d5\u30a1\u30f3\u6b8b\u57fa\u304c\u5b58\u5728\u3059\u308b\u3068\u9084\u5143\u53cd\u5fdc\u304c\u8d77\u3053\u308a\u3084\u3059\u304f\u306a\u308a\u307e\u3059\uff09\u3002\u307e\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5358\u4f4d\u8cea\u91cf\u3042\u305f\u308a\u306b\u5b58\u5728\u3059\u308b\u30da\u30d7\u30c1\u30c9\u7d50\u5408\u306e\u6570\u306f\u307b\u307c\u4e00\u5b9a\u3067\u3042\u308b\u305f\u3081\u3001BCA\/Cu+ \u8907\u5408\u4f53\u306e\u767a\u8272\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u306b\u6bd4\u4f8b\u3057\u307e\u3059\u3002\u5b9f\u969bBCA\u6cd5\u3067\u306f\u76f4\u7dda\u6027\u306e\u9ad8\u3044\u691c\u91cf\u7dda\uff08r2&gt;0.95\uff09\u304c\u5f97\u3089\u308c\u307e\u3059\u3002<strong>BCA\/Cu+ \u8907\u5408\u4f53\u306f\u6c34\u6eb6\u6027\u3067562 nm\u306b\u5438\u53ce\u3092\u3082\u3061\u3001\u3053\u306e\u767a\u8272\u5f37\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u3092\u898b\u7a4d\u3082\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/strong><\/p>\n<h3>BCA\u6cd5\u306e\u30e1\u30ea\u30c3\u30c8<\/h3>\n<ul>\n<li>\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u504f\u5dee\u304c\u5c0f\u3055\u3044\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7a2e\u985e\u306b\u3088\u308b\u767a\u8272\u306e\u3070\u3089\u3064\u304d\u304c\u5c11\u306a\u3044\uff09<\/li>\n<li>\u754c\u9762\u6d3b\u6027\u5264\u3068\u306e\u5171\u5b58\u6027\u304c\u9ad8\u3044<\/li>\n<li>\u691c\u91cf\u7dda\u306e\u76f4\u7dda\u6027\u304c\u9ad8\u3044\uff08r2 &gt; 0.95\uff09<\/li>\n<li>\u5ba4\u6e29\u4fdd\u5b58\u306e\u305f\u3081\u3001\u3059\u3050\u306b\u4f7f\u7528\u53ef\u80fd<\/li>\n<\/ul>\n<h3>BCA\u6cd5\u306e\u30c7\u30e1\u30ea\u30c3\u30c8<\/h3>\n<ul>\n<li>\u9084\u5143\u5264\u3084\u9285\u30a4\u30aa\u30f3\u306e\u30ad\u30ec\u30fc\u30c8\u5264\u306b\u3088\u308a\u53cd\u5fdc\u304c\u963b\u5bb3<\/li>\n<li>\u5ba4\u6e29\u3067\u3082\u767a\u8272\u306f\u5c11\u3057\u9032\u3080\u305f\u3081\u6e2c\u5b9a\u6642\u9593\u306b\u6ce8\u610f\u304c\u5fc5\u8981<\/li>\n<\/ul>\n<h2>Bradford (Coomassie)\u6cd5<\/h2>\n<p>Coomassie Blue\u8272\u7d20\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7d50\u5408\u3059\u308b\u3053\u3068\u304c1963\u5e74Groth\u3089\u306b\u3088\u3063\u3066\u5831\u544a\u3055\u308c\u3001\u305d\u306e\u5f8c1976\u5e74\u306bMarion Bradford\u3089\u306b\u3088\u3063\u3066Coomassie G-250\u3092\u7528\u3044\u305f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u304c\u7d39\u4ecb\u3055\u308c\u307e\u3057\u305f\u3002\u5f0a\u793e\u306e<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/23200?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Coomassie \uff08Bradford\uff09 Protein Assay Kit<\/a>\u3084<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/23236?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Coomassie Plus \uff08Bradford\uff09 Protein Assay Kit<\/a>\u306f\u3001Marion Bradford\u3089\u306e\u65b9\u6cd5\u3092\u30d9\u30fc\u30b9\u3068\u3057\u3066\u6539\u826f\u3092\u52a0\u3048\u305f\u88fd\u54c1\u3067\u3059\u3002<\/p>\n<h3>Bradford (Coomassie)\u6cd5\u306e\u5316\u5b66\u53cd\u5fdc<\/h3>\n<p>Coomassie G-250\u306f\u9178\u6027\u6761\u4ef6\u4e0b\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7d50\u5408\u3057\u307e\u3059\u3002Coomassie\u8272\u7d20\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7d50\u5408\u3059\u308b\u3068\u8272\u7d20\u306f\u8d64\u8336\u8272\uff08\u6975\u5927\u5438\u53ce\u6ce2\u9577: 465 nm\uff09\u304b\u3089\u9752\u8272\uff08\u6975\u5927\u5438\u53ce\u6ce2\u9577: 610 nm\uff09\u306b\u5909\u5316\u3057\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7d50\u5408\u3057\u3066\u3044\u306a\u3044\u6642\u3068\u3057\u3066\u3044\u308b\u6642\u306e\u5438\u5149\u5ea6\u306e\u5dee\u304c\u6700\u3082\u5927\u304d\u304f\u306a\u308b\u306e\u304c595 nm\u4ed8\u8fd1\u3067\u3042\u308b\u3053\u3068\u304b\u3089\u3001<strong>Coomassie\u8272\u7d20\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306e\u8907\u5408\u4f53\u306e\u5438\u5149\u5ea6\u306f\u4e00\u822c\u7684\u306b595 nm\u3067\u6e2c\u5b9a\u3057\u307e\u3059<\/strong>\uff08\u56f33\uff09\u3002\u9752\u8272\u306eCoomassie\/\u30bf\u30f3\u30d1\u30af\u8cea\u8907\u5408\u4f53\u306e\u5438\u5149\u5ea6\u306f575 nm\u304b\u3089615nm\u306e\u9593\u3067\u3082\u6e2c\u5b9a\u304c\u53ef\u80fd\u3067\u3059\u3002\u305f\u3060\u3057575 nm \u3084615 nm\u3067\u6e2c\u5b9a\u3057\u305f\u5834\u5408\u3001\u5438\u5149\u5ea6\u306f595 nm\u3067\u6e2c\u5b9a\u3059\u308b\u5834\u5408\u306b\u6bd4\u3079\u306610%\u7a0b\u5ea6\u4f4e\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<div id=\"attachment_160\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-160\" class=\"wp-image-160 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-fig3.jpg\" alt=\"Bradford (Coomassie)\u6cd5\u306e\u53cd\u5fdc\u30b9\u30ad\u30fc\u30e0\" width=\"500\" height=\"288\" \/><p id=\"caption-attachment-160\" class=\"wp-caption-text\">\u56f33 Bradford (Coomassie)\u6cd5\u306e\u53cd\u5fdc\u30b9\u30ad\u30fc\u30e0<\/p><\/div>\n<p>Coomassie\u8272\u7d20\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5869\u57fa\u6027\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\uff08\u7279\u306b\u30a2\u30eb\u30ae\u30cb\u30f3\u6b8b\u57fa\u3001\u30ea\u30b8\u30f3\u6b8b\u57fa\u3001\u30d2\u30b9\u30c1\u30b8\u30f3\u6b8b\u57fa\uff09\u3084N \u672b\u7aef\u30a2\u30df\u30ce\u9178\u3068\u7d50\u5408\u3059\u308b\u4ed6\u3001\u82b3\u9999\u65cf\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u3068\u758e\u6c34\u7d50\u5408\uff08\u307e\u305f\u306f\u30d5\u30a1\u30f3\u30c7\u30eb\u30ef\u30fc\u30eb\u30b9\u7d50\u5408\uff09 \u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u5b50\u306b\u5bfe\u3059\u308bCoomassie\u8272\u7d20\u306e\u7d50\u5408\u91cf\u306f\u57fa\u672c\u7684\u306b\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u5b50\u306b\u5b58\u5728\u3059\u308b\u30d7\u30e9\u30b9\u96fb\u8377\u306e\u6570\u306b\u307b\u307c\u6bd4\u4f8b\u3057\u307e\u3059\u3002\u3057\u304b\u3057\u30d5\u30ea\u30fc\u306e\u30a2\u30df\u30ce\u9178\u3084\u30da\u30d7\u30c1\u30c9\u3068\u3067\u306fCoomassie\u8272\u7d20\u306e\u7d50\u5408\u306b\u3088\u308b\u767a\u8272\u306f\u8d77\u3053\u3089\u305a\u3001<strong>\u4e00\u822c\u7684\u306b\u306f\u30da\u30d7\u30c1\u30c9\uff08\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u306e\u5206\u5b50\u91cf\u306f\u5c11\u306a\u304f\u3068\u30823,000 Da\u4ee5\u4e0a\u304c\u5fc5\u8981<\/strong>\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u70b9\u306f\u4f4e\u5206\u5b50\u306e\u30da\u30d7\u30c1\u30c9\u3092\u7121\u8996\u3057\u3066\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u91cf\u3092\u6e2c\u5b9a\u3057\u305f\u3044\u5834\u5408\u3001\u4f8b\u3048\u3070\u30d3\u30fc\u30eb\u5de5\u5834\u306e\u767a\u9175\u904e\u7a0b\u3067\u9ad8\u5206\u5b50\u91cf\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u91cf\u3092\u6e2c\u5b9a\u3059\u308b\u5834\u5408\u306a\u3069\u3067\u306f\u30e1\u30ea\u30c3\u30c8\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<h3>Bradford (Coomassie)\u6cd5\u306e\u30e1\u30ea\u30c3\u30c8<\/h3>\n<ul>\n<li>\u53cd\u5fdc\u6642\u9593\u304c\u77ed\u304f\u64cd\u4f5c\u304c\u7c21\u4fbf<\/li>\n<li>\u9084\u5143\u5264\u3084\u5869\u3068\u306e\u5171\u5b58\u6027\u304c\u9ad8\u3044<\/li>\n<\/ul>\n<h3>Bradford (Coomassie)\u6cd5\u306e\u30c7\u30e1\u30ea\u30c3\u30c8<\/h3>\n<ul>\n<li>\u691c\u91cf\u7dda\u306e\u76f4\u7dda\u6027\u304c\u4f4e\u3044<\/li>\n<li>\u754c\u9762\u6d3b\u6027\u5264\u306b\u3088\u308a\u53cd\u5fdc\u304c\u963b\u5bb3<\/li>\n<li>\u5b89\u5b9a\u4fdd\u7ba1\u306b\u306f\u51b7\u8535\u4fdd\u7ba1\u304c\u5fc5\u8981*\uff08\u4f7f\u7528\u524d\u306b\u5ba4\u6e29\u306b\u623b\u3059\u5fc5\u8981\u3042\u308a\uff09<\/li>\n<li>Coomassie\u8272\u7d20\u306f\u30ac\u30e9\u30b9\u3084\u77f3\u82f1\u306e\u30ad\u30e5\u30d9\u30c3\u30c8\u306b\u5438\u7740<\/li>\n<\/ul>\n<p><span style=\"font-size: 8pt\">\u203b1\u30f6\u6708\u4ee5\u5185\u3067\u3042\u308c\u3070\u5ba4\u6e29\uff0818-26\u2103\uff09\u3067\u4fdd\u5b58\u304c\u53ef\u80fd<\/span><\/p>\n<h2>Lowry\u6cd5<\/h2>\n<p>Lowry\u6cd5\u306fOliver H. Lowry\u3089\u306b\u3088\u3063\u30661951\u5e74\u306b\u7d39\u4ecb\u3055\u308c\u305f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u3067\u3059\u3002Lowry\u6cd5\u306f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u305f<strong>\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u6cd5\u3092\u6539\u826f\u3057\u3066\u611f\u5ea6\u3092\u98db\u8e8d\u7684\u306b\u9ad8\u3081\u305f\u65b9\u6cd5<\/strong>\u3067\u3059\u3002Lowry\u3089\u306e\u8ad6\u6587\u306f\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u95a2\u9023\u306e\u8ad6\u6587\u306e\u306a\u304b\u3067\u6700\u3082\u5f15\u7528\u6570\u306e\u591a\u3044\u8ad6\u6587\u306e\u3072\u3068\u3064\u3067\u3059\u3002\u5f0a\u793e\u306e<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/23240?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Modified Lowry Protein Assay Kit<\/a>\u306f\u3001Lowry\u3089\u304c\u4f7f\u7528\u3057\u3066\u3044\u305f\u4e0d\u5b89\u5b9a\u306a2\u3064\u306e\u8a66\u85ac\u30921\u3064\u306e\u5b89\u5b9a\u306a\u8a66\u85ac\u306b\u7f6e\u304d\u63db\u3048\u3001\u3088\u308a\u7c21\u4fbf\u306b\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u304c\u884c\u3048\u308b\u3088\u3046\u306b\u6539\u826f\u3057\u305f\u88fd\u54c1\u3067\u3059\u3002<\/p>\n<h3>Modified Lowry Protein Assay\u306e\u5316\u5b66\u53cd\u5fdc<\/h3>\n<p>\u3082\u3068\u3082\u3068\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u6cd5\u3092\u6539\u826f\u3057\u305f\u65b9\u6cd5\u3067\u3042\u308b\u305f\u3081\u3001Modified Lowry Protein Assay\u306e\u5316\u5b66\u53cd\u5fdc\u306fBCA\u6cd5\u3068\u4f3c\u3066\u3044\u307e\u3059\u304c\u3001BCA\u3067\u306f\u306a\u304fFolin phenol\u8a66\u85ac\u3092\u5229\u7528\u3059\u308b\u70b9\u3067\u5927\u304d\u304f\u7570\u306a\u308a\u307e\u3059\u3002Modified Lowry Protein Assay\u306f2\u3064\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u53cd\u5fdc\u304c\u8d77\u3053\u308a\u307e\u3059\uff08\u56f34\uff09\u3002\u6700\u521d\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u306fBCA\u6cd5\u3068\u540c\u69d8\u306b\u3001\u9152\u77f3\u9178\u5869\u3092\u542b\u3080\u30a2\u30eb\u30ab\u30ea\u6761\u4ef6\u4e0b\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u306e4\u3064\u304b\u30896\u3064\u306e\u30da\u30d7\u30c1\u30c9\u7d50\u5408\u3042\u305f\u308a1\u5206\u5b50\u306e\u9285\u30a4\u30aa\u30f3\u3068\u9752\u8272\u306e\u56db\u5ea7\u914d\u4f4d\u8907\u5408\u4f53\u3092\u5f62\u6210\u3059\u308b\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u304c\u8d77\u3053\u308a\u307e\u3059\u3002Lowry\u6cd5\u3067\u306f\u6b21\u306bFolin phenol\u8a66\u85ac\u3092\u53cd\u5fdc\u6db2\u306b\u6dfb\u52a0\u3057\u307e\u3059\u3002Folin phenol\u8a66\u85ac\u304c\u53cd\u5fdc\u6db2\u306b\u52a0\u3048\u3089\u308c\u308b\u3068\u9752\u8272\u306e\u767a\u8272\u5f37\u5ea6\u304c\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u53cd\u5fdc\u306f\u3001Folin\u8a66\u85ac\uff08\u30ea\u30f3\u30e2\u30ea\u30d6\u30c7\u30f3\u9178\uff0f\u30ea\u30f3\u30bf\u30f3\u30b0\u30b9\u30c6\u30f3\u9178\uff09\u304c\u56db\u5ea7\u914d\u4f4d\u8907\u5408\u4f53\u306b\u3088\u308a\u9084\u5143\u3055\u308c\u308b\u3053\u3068\u3067\u8d77\u3053\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3068\u304d\u306e\u9752\u8272\u306e\u767a\u8272\u306f\u5ba4\u6e29\u3067\u7d0430\u5206\u9593\u6301\u7d9a\u3057\u307e\u3059\u3002Lowry\u3089\u3084Legler\u3089\u306b\u3088\u308c\u3070\u3001\u30d3\u30e5\u30fc\u30ec\u30c3\u30c8\u53cd\u5fdc\u6642\u306e\u4e0d\u5b89\u5b9a\u306a\u8907\u5408\u4f53\u304b\u3089\u5b89\u5b9a\u306a\u8907\u5408\u4f53\u3078\u306e\u69cb\u9020\u5909\u5316\u304c\u3053\u306e30\u5206\u9593\u306b\u8d77\u304d\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u30c1\u30ed\u30b7\u30f3\u6b8b\u57fa\u3001\u30c8\u30ea\u30d7\u30c8\u30d5\u30a1\u30f3\u6b8b\u57fa\u3001\u30b7\u30b9\u30c6\u30a4\u30f3\u6b8b\u57fa\u3001\u30d2\u30b9\u30c1\u30b8\u30f3\u6b8b\u57fa\u3001\u30a2\u30b9\u30d1\u30e9\u30ae\u30f3\u6b8b\u57fa\u306e5\u3064\u306e\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u306f\u30ea\u30f3\u30e2\u30ea\u30d6\u30c7\u30f3\u9178\uff0f\u30ea\u30f3\u30bf\u30f3\u30b0\u30b9\u30c6\u30f3\u9178\u8907\u5408\u4f53\u306e\u9084\u5143\u306b\u95a2\u4e0e\u3059\u308b\u305f\u3081\u3001\u30da\u30d7\u30c1\u30c9\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u4e2d\u306b\u3053\u308c\u3089\u306e\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u304c\u5b58\u5728\u3059\u308b\u3068\u767a\u8272\u5f37\u5ea6\u304c\u3088\u308a\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002\u307e\u305f\u30c1\u30ed\u30b7\u30f3\u3068\u30c8\u30ea\u30d7\u30c8\u30d5\u30a1\u30f3\u4ee5\u5916\u306e\u30d5\u30ea\u30fc\u306e\u30a2\u30df\u30ce\u9178\u3067\u306f\u767a\u8272\u306f\u8d77\u3053\u308a\u307e\u305b\u3093\u304c\u3001\u901a\u5e38\u30da\u30d7\u30c1\u30c9\u7d50\u5408\u304c2\u3064\u4ee5\u4e0a\u3042\u308c\u3070\u767a\u8272\u304c\u691c\u51fa\u3067\u304d\u308b\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<div id=\"attachment_161\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-161\" class=\"wp-image-161 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-fig4.jpg\" alt=\"Lowry\u6cd5\u306e\u53cd\u5fdc\u30b9\u30ad\u30fc\u30e0\" width=\"500\" height=\"261\" \/><p id=\"caption-attachment-161\" class=\"wp-caption-text\">\u56f34 Lowry\u6cd5\u306e\u53cd\u5fdc\u30b9\u30ad\u30fc\u30e0<\/p><\/div>\n<h3>Lowry\u6cd5\u306e\u30e1\u30ea\u30c3\u30c8<\/h3>\n<ul>\n<li>\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u504f\u5dee\u304c\u5c0f\u3055\u3044\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7a2e\u985e\u306b\u3088\u308b\u767a\u8272\u306e\u3070\u3089\u3064\u304d\u304c\u5c11\u306a\u3044<sup>\u203b<\/sup>\uff09<br \/>\n\u203bBradford\u6cd5\u306e\u3088\u3046\u306b\u3001\u8907\u6570\u306e\u7279\u5b9a\u30a2\u30df\u30ce\u9178\u306b\u7d50\u5408\u3059\u308b\u624b\u6cd5\u306b\u6bd4\u3079\u308b\u3068\u3070\u3089\u3064\u304d\u306f\u5c11\u306a\u3044\u3067\u3059\u304c\u3001<br \/>\n\u4e0a\u8ff0\u306e\u3088\u3046\u306b\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d44\u6210\u304c\u767a\u8272\u5f37\u5ea6\u306b\u5f71\u97ff\u3059\u308b\u305f\u3081\u3001BCA\u6cd5\u3068\u6bd4\u3079\u308b\u3068\u767a\u8272\u306e\u3070\u3089\u3064\u304d\u306f\u5927\u304d\u3044\u3067\u3059\u3002<\/li>\n<\/ul>\n<h3>Lowry\u6cd5\u306e\u30c7\u30e1\u30ea\u30c3\u30c8<\/h3>\n<ul>\n<li>\u64cd\u4f5c\u304c\u7169\u96d1<\/li>\n<li>\u539f\u6cd5\u3067\u306f\u963b\u5bb3\u7269\u8cea\u304c\u591a\u3044\uff08\u9084\u5143\u5264\u3001\u754c\u9762\u6d3b\u6027\u5264\u3001EDTA\u306a\u3069\u306e\u30ad\u30ec\u30fc\u30c8\u5264\u3001\u30ab\u30ea\u30a6\u30e0\u30a4\u30aa\u30f3\u3001Tris\u30d0\u30c3\u30d5\u30a1\u30fc\u306a\u3069\uff09<\/li>\n<li>\u5b89\u5b9a\u4fdd\u7ba1\u306b\u306f\u51b7\u8535\u4fdd\u7ba1\u304c\u5fc5\u8981*\uff08\u4f7f\u7528\u524d\u306b\u5ba4\u6e29\u306b\u623b\u3059\u5fc5\u8981\u3042\u308a\uff09<\/li>\n<\/ul>\n<p><span style=\"font-size: 8pt\">\u203b 1\u30f6\u6708\u4ee5\u5185\u3067\u3042\u308c\u3070\u5ba4\u6e29\uff0818-26\u2103\uff09\u3067\u4fdd\u5b58\u304c\u53ef\u80fd<\/span><\/p>\n<h2>Pierce 660 nm Protein Assay\u6cd5<\/h2>\n<p><a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/22662?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce 660 nm Protein Assay<\/a> \u8a66\u85ac\u306f\u3001<strong>Bradford \uff08Coomassie\uff09\u6cd5\u306e\u7c21\u4fbf\u3055\u3068\u754c\u9762\u6d3b\u6027\u5264\u3068\u306e\u5171\u5b58\u6027\u3092\u517c\u306d\u5099\u3048\u305f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u8a66\u85ac<\/strong>\u3067\u3059\u3002\u76f4\u7dda\u6027\u304c\u9ad8\u304f\u3001\u30a6\u30b7\u8840\u6e05\u30a2\u30eb\u30d6\u30df\u30f3\uff08BSA\uff09\u3092\u7528\u3044\u305f\u691c\u91cf\u7dda\u3067\u306f25-2,000 \u00b5g\/mL\uff08\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u3092\u7528\u3044\u308b\u30d7\u30ed\u30c8\u30b3\u30eb\u3067\u306f50-2,000 \u00b5g\/mL\uff09\u3068Bradford \uff08Coomassie\uff09\u6cd5\u3088\u308a\u3082\u5e83\u3044\u30ec\u30f3\u30b8\u3067\u6e2c\u5b9a\u304c\u53ef\u80fd\u3067\u3059\u3002\u307e\u305f\u5225\u9014<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/22663?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Ionic Detergent Compatibility Reagent<\/a>\u3092\u52a0\u3048\u308b\u3053\u3068\u3067\u3001\u4ed6\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u3067\u306f\u56f0\u96e3\u306a<strong>Laemmli SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6eb6\u89e3\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u6e2c\u5b9a\u3082\u53ef\u80fd<\/strong>\u3067\u3059\u3002<\/p>\n<p>Pierce 660 nm Protein Assay \u306f\u3001\u72ec\u81ea\u958b\u767a\u306e\u8272\u7d20\u30fb\u91d1\u5c5e\u8907\u5408\u4f53\u304c\u9178\u6027\u6761\u4ef6\u4e0b\u306b\u304a\u3044\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7d50\u5408\u3059\u308b\u3053\u3068\u3067\u3001\u8d64\u8336\u8272\u304b\u3089\u7dd1\u8272\uff08\u6975\u5927\u5438\u53ce\u6ce2\u9577: 660 nm\uff09\u306b\u5909\u5316\u3059\u308b\u5316\u5b66\u53cd\u5fdc\u3092\u5229\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u8272\u7d20\u30fb\u91d1\u5c5e\u8907\u5408\u4f53\u306f\u4e3b\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5869\u57fa\u6027\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\uff08\u7279\u306b\u30a2\u30eb\u30ae\u30cb\u30f3\u6b8b\u57fa\u3001\u30ea\u30b8\u30f3\u6b8b\u57fa\u3001\u30d2\u30b9\u30c1\u30b8\u30f3\u6b8b\u57fa\uff09\u306b\u7d50\u5408\u3057\u307e\u3059\u3002\u307e\u305f\u30c1\u30ed\u30b7\u30f3\u6b8b\u57fa\u3084\u30c8\u30ea\u30d7\u30c8\u30d5\u30a1\u30f3\u6b8b\u57fa\u306e\u4ed6\u3001\u30d5\u30a7\u30cb\u30eb\u30a2\u30e9\u30cb\u30f3\u6b8b\u57fa\u306b\u3082\uff08\u5f31\u304f\uff09\u7d50\u5408\u3057\u307e\u3059\u3002<\/p>\n<p>\u56f35\u306f\u30a6\u30b7\u8840\u6e05\u30a2\u30eb\u30d6\u30df\u30f3\uff08BSA\uff09\u3068\u306e\u7d50\u5408\u3067\u5909\u5316\u3059\u308b\u8272\u7d20\u30fb\u91d1\u5c5e\u8907\u5408\u4f53\u306e\u5438\u53ce\u30b9\u30da\u30af\u30c8\u30eb\u3092\u8868\u3057\u3066\u3044\u307e\u3059\u3002\u8272\u7d20\u306e\u307f\uff08\u91d1\u5c5e\u306a\u3057\uff09\u306e\u5834\u5408\u306f660 nm\u3067\u306e\u5438\u53ce\u306f\u307b\u3068\u3093\u3069\u898b\u3089\u308c\u307e\u305b\u3093\u3002BSA\u304c\u5b58\u5728\u3057\u3066\u3082\u91d1\u5c5e\u3092\u6dfb\u52a0\u3057\u306a\u3051\u308c\u3070\u8272\u7d20\u306e\u307f\u306e\u5834\u5408\u3068\u540c\u69d8\u306b660 nm\u3067\u306e\u5438\u53ce\u306f\u307b\u3068\u3093\u3069\u898b\u3089\u308c\u307e\u305b\u3093\u304c\u3001\u8272\u7d20\u30fb\u91d1\u5c5e\u8907\u5408\u4f53\u3092\u7528\u3044\u308b\u3068660 nm\u306b\u5438\u53ce\u304c\u3042\u308aBSA\u306e\u6fc3\u5ea6\u306b\u4f9d\u5b58\u3057\u3066\u5438\u5149\u5ea6\u304c\u9ad8\u304f\u306a\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002<\/p>\n<div id=\"attachment_162\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-162\" class=\"wp-image-162 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-fig5.jpg\" alt=\"\u30a6\u30b7\u8840\u6e05\u30a2\u30eb\u30d6\u30df\u30f3\uff08BSA\uff09\u3078\u306e\u7d50\u5408\u306b\u3088\u308b\u8272\u7d20\uff0f\u91d1\u5c5e\u8907\u5408\u4f53\u306e\u5438\u53ce\u30b9\u30da\u30af\u30c8\u30eb\u5909\u5316\" width=\"500\" height=\"265\" \/><p id=\"caption-attachment-162\" class=\"wp-caption-text\">\u56f35 \u30a6\u30b7\u8840\u6e05\u30a2\u30eb\u30d6\u30df\u30f3\uff08BSA\uff09\u3078\u306e\u7d50\u5408\u306b\u3088\u308b\u8272\u7d20\uff0f\u91d1\u5c5e\u8907\u5408\u4f53\u306e\u5438\u53ce\u30b9\u30da\u30af\u30c8\u30eb\u5909\u5316<\/p><\/div>\n<p style=\"text-align: left\">Varian Cary spectrophotometer\u3092\u7528\u3044\u3066Pierce 660 nm Protein Assay Reagent\u306e\u5438\u53ce\u30b9\u30da\u30af\u30c8\u30eb\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002\u72ec\u81ea\u958b\u767a\u306e\u8272\u7d20\uff0f\u91d1\u5c5e\u8907\u5408\u4f53\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7d50\u5408\u3059\u308b\u3068\u5438\u53ce\u6975\u5927\u304c\u9577\u6ce2\u9577\u5074\u3078\u5927\u304d\u304f\u30b7\u30d5\u30c8\u3057\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<h3>Pierce 660 nm Protain Assay\u6cd5\u306e\u7279\u9577<\/h3>\n<ul>\n<li>\u53cd\u5fdc\u6642\u9593\u304c\u77ed\u304f\u64cd\u4f5c\u304c\u7c21\u4fbf<\/li>\n<li>\u691c\u91cf\u7dda\u306e\u76f4\u7dda\u6027\u304c\u9ad8\u3044\uff08\u56f36\uff09<\/li>\n<li>\u9084\u5143\u5264\u3084\u754c\u9762\u6d3b\u6027\u5264\u3068\u306e\u5171\u5b58\u6027\u304c\u9ad8\u3044<\/li>\n<li>\u5ba4\u6e29\u4fdd\u5b58\u306e\u305f\u3081\u3001\u3059\u3050\u306b\u4f7f\u7528\u53ef\u80fd<\/li>\n<\/ul>\n<div id=\"attachment_163\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-163\" class=\"wp-image-163 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-fig6.jpg\" alt=\"Pierce 660 nm Protein Assay \u3068 Bradford Protein Assay \uff08B\u793e\uff09\u306e\u76f4\u7dda\u6027\u6bd4\u8f03\" width=\"500\" height=\"312\" \/><p id=\"caption-attachment-163\" class=\"wp-caption-text\">\u56f36 Pierce 660 nm Protein Assay \u3068 Bradford Protein Assay \uff08B\u793e\uff09\u306e\u76f4\u7dda\u6027\u6bd4\u8f03<\/p><\/div>\n<p style=\"text-align: left\">\u305d\u308c\u305e\u308c\u30c6\u30b9\u30c8\u30c1\u30e5\u30fc\u30d6\u3092\u7528\u3044\u308b\u30d7\u30ed\u30c8\u30b3\u30eb\u3067BSA\u306e\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3057\u307e\u3057\u305f\u3002Pierce 660 nm Protein Assay \uff08\u9752\u8272\uff09\u3067\u306f 25-2,000 \u00b5g\/mL \u306e\u7bc4\u56f2\u3067\u76f4\u7dda\u6027\u304c\u5f97\u3089\u308c\u305f\u306e\u306b\u5bfe\u3057\u3001Bradford Assay \uff08\u9ec4\u8272\uff09\u3067\u306f 125-1,000 \u00b5g\/mL\u306e\u7bc4\u56f2\u3067\u306e\u307f\u76f4\u7dda\u6027\u3092\u793a\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>&nbsp;<\/p>\n<h2>\u6700\u5f8c\u306b<\/h2>\n<p>\u6e2c\u5b9a\u30b5\u30f3\u30d7\u30eb\u306b\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u4ee5\u5916\u306b\u3082\u591a\u304f\u306e\u5206\u5b50\u304c\u542b\u307e\u308c\u3066\u304a\u308a\u3001\u3053\u308c\u3089\u304c\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u3092\u963b\u5bb3\u3059\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u5404\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306b\u304a\u3044\u3066\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u5171\u5b58\u304c\u53ef\u80fd\u306a\u7269\u8cea\u3068\u305d\u306e\u6fc3\u5ea6\u306b\u3064\u3044\u3066\u306f\u3001\u5404\u88fd\u54c1\u6dfb\u4ed8\u66f8\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002\u307e\u305f\u5171\u5b58\u53ef\u80fd\u6fc3\u5ea6\u3092\u8d85\u3048\u308b\u7269\u8cea\u3084\u5171\u5b58\u6027\u304c\u4e0d\u660e\u306a\u7269\u8cea\u304c\u542b\u307e\u308c\u308b\u5834\u5408\u306f\u3001\u6e2c\u5b9a\u524d\u306b\u9664\u53bb\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u9664\u53bb\u65b9\u6cd5\u3068\u3057\u3066\u306f\u3001\u900f\u6790\u3001\u30b2\u30eb\u308d\u904e\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u6c88\u6bbf\u306e\u3044\u305a\u308c\u304b\u304c\u5229\u7528\u3067\u304d\u307e\u3059\u3002\u5f0a\u793e\u3067\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u6c88\u6bbf\u6cd5\u3092\u5229\u7528\u3057\u305f\u963b\u5bb3\u5264\u9664\u53bb\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u3092\u884c\u3046\u305f\u3081\u306e\u30ad\u30c3\u30c8\u3068\u3057\u3066\u3001<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/23229?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Compat-Able Protein Assay Kits<\/a>\u3082\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3053\u308c\u307e\u3067\u6bd4\u8272\u6cd5\u3092\u30d9\u30fc\u30b9\u3068\u3057\u305f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u3066\u304d\u307e\u3057\u305f\u3002\u6bd4\u8272\u6cd5\u4ee5\u5916\u306b\u306fUV\u6cd5\u3084\u86cd\u5149\u6cd5\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u305d\u308c\u305e\u308c\u3069\u3093\u306a\u6642\u306b\u6709\u7528\u306a\u306e\u304b\u3001\u3054\u8208\u5473\u3042\u308b\u65b9\u306f<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic24_bid_ts\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u95a2\u9023\u8a18\u4e8b<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u3053\u306e\u30d6\u30ed\u30b0\u3092\u8aad\u3093\u3060\u4eba\u306f\u3053\u3093\u306a\u30d6\u30ed\u30b0\u3082\u8aad\u3093\u3067\u3044\u307e\u3059\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic24_bid_ts\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u307e\u3068\u3081\u7d9a\u304d\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01UV\u6cd5\u3084\u86cd\u5149\u6cd5\u3092\u4f7f\u3046\u306e\u306f\u3069\u3093\u306a\u3068\u304d\uff1f<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic5\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u900f\u6790\u3001\u30b2\u30eb\u308d\u904e\u3001\u9650\u5916\u308d\u904e\u306a\u3069\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u593e\u96d1\u6210\u5206\u306e\u9664\u53bb\u65b9\u6cd5\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c5\u56de<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-assay-compatibility\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3042\u306a\u305f\u306e\u30b5\u30f3\u30d7\u30eb\u306f\u5927\u4e08\u592b\uff1f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u306e\u9069\u5408\u6027\u5224\u5b9a\u65b9\u6cd5<\/a><\/p>\n<h2>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/a><\/p>\n<p>&nbsp;<\/p>\n<h2>\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u95a2\u9023\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic1\/??CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c1\u56de\u3011\u754c\u9762\u6d3b\u6027\u5264\u3092\u7528\u3044\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic2\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c2\u56de\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u306b\u4f7f\u7528\u3059\u308b\u754c\u9762\u6d3b\u6027\u5264\u306e\u7279\u6027\u3068\u7a2e\u985e<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic3\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c3\u56de\u3011\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u7a2e\u985e\u3092\u307e\u3068\u3081\u3066\u307f\u305f<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic5\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c5\u56de\u3011\u900f\u6790\u3001\u30b2\u30eb\u308d\u904e\u3001\u9650\u5916\u308d\u904e\u306a\u3069\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u593e\u96d1\u6210\u5206\u306e\u9664\u53bb\u65b9\u6cd5\u307e\u3068\u3081<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic19\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c19\u56de\u3011\u8cea\u91cf\u5206\u6790\u8a08\u306e\u57fa\u672c\u306e\u300c\u304d\u300d<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic20\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c20\u56de\u3011\u5b9a\u91cf\u30d7\u30ed\u30c6\u30aa\u30df\u30af\u30b9\u3068\u306f\uff1f<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic24_bid_ts\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa12_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u307e\u3068\u3081\uff1a\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01UV\u6cd5\u3084\u86cd\u5149\u6cd5\u3092\u4f7f\u3046\u306e\u306f\u3069\u3093\u306a\u3068\u304d\uff1f<br \/>\n<\/a><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7b2c4\u56de\u76ee\u306e\u4eca\u56de\u306f\u3001\u524d\u56de\u306b\u5f15\u304d\u3064\u3065\u304d\u300c\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u300d\u3092\u30c6\u30fc\u30de\u3068\u3057\u3066\u3001BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u3092\u5143\u306b\u3057\u305f\u5f0a\u793e\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u8a66\u85ac\u88fd\u54c1\u306e\u539f\u7406\u3084\u7279\u5fb4\u306b\u3064\u3044\u3066\u89e3\u8aac\u3057\u307e\u3059\uff01<\/p>\n","protected":false},"author":988,"featured_media":2083,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[156],"tags":[237,21],"division":[],"class_list":{"0":"post-60","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-protein-experiments","9":"tag-protein-analysis","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.4 (Yoast SEO v27.4) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de\" \/>\n<meta property=\"og:description\" content=\"\u7b2c4\u56de\u76ee\u306e\u4eca\u56de\u306f\u3001\u524d\u56de\u306b\u5f15\u304d\u3064\u3065\u304d\u300c\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u300d\u3092\u30c6\u30fc\u30de\u3068\u3057\u3066\u3001BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u3092\u5143\u306b\u3057\u305f\u5f0a\u793e\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u8a66\u85ac\u88fd\u54c1\u306e\u539f\u7406\u3084\u7279\u5fb4\u306b\u3064\u3044\u3066\u89e3\u8aac\u3057\u307e\u3059\uff01\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/\" \/>\n<meta property=\"og:site_name\" content=\"Learning at the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/ThermoFisherJapan\" \/>\n<meta property=\"article:published_time\" content=\"2020-03-03T15:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-05-19T03:27:09+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-title-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"630\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"LATB Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:site\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"LATB Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"19\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/\"},\"author\":{\"name\":\"LATB Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\"},\"headline\":\"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de\",\"datePublished\":\"2020-03-03T15:00:00+00:00\",\"dateModified\":\"2025-05-19T03:27:09+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/\"},\"wordCount\":296,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/protein-basic4-title-1.jpg\",\"keywords\":[\"\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\",\"\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\"],\"articleSection\":[\"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023\"],\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/\",\"name\":\"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de - Learning at the Bench\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/protein-basic4-title-1.jpg\",\"datePublished\":\"2020-03-03T15:00:00+00:00\",\"dateModified\":\"2025-05-19T03:27:09+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/#breadcrumb\"},\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/protein-basic4-title-1.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/protein-basic4-title-1.jpg\",\"width\":1200,\"height\":630,\"caption\":\"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/protein-basic4\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\",\"name\":\"Learning at the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"ja\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\",\"name\":\"LATB Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"caption\":\"LATB Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/latbstaff\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de - Learning at the Bench","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/","og_locale":"ja_JP","og_type":"article","og_title":"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de","og_description":"\u7b2c4\u56de\u76ee\u306e\u4eca\u56de\u306f\u3001\u524d\u56de\u306b\u5f15\u304d\u3064\u3065\u304d\u300c\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u300d\u3092\u30c6\u30fc\u30de\u3068\u3057\u3066\u3001BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u3092\u5143\u306b\u3057\u305f\u5f0a\u793e\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u8a66\u85ac\u88fd\u54c1\u306e\u539f\u7406\u3084\u7279\u5fb4\u306b\u3064\u3044\u3066\u89e3\u8aac\u3057\u307e\u3059\uff01","og_url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/","og_site_name":"Learning at the Bench","article_publisher":"https:\/\/www.facebook.com\/ThermoFisherJapan","article_published_time":"2020-03-03T15:00:00+00:00","article_modified_time":"2025-05-19T03:27:09+00:00","og_image":[{"width":1200,"height":630,"url":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-title-1.jpg","type":"image\/jpeg"}],"author":"LATB Staff","twitter_card":"summary_large_image","twitter_creator":"@ThermoFisherJP","twitter_site":"@ThermoFisherJP","twitter_misc":{"\u57f7\u7b46\u8005":"LATB Staff","\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593":"19\u5206"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/"},"author":{"name":"LATB Staff","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d"},"headline":"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de","datePublished":"2020-03-03T15:00:00+00:00","dateModified":"2025-05-19T03:27:09+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/"},"wordCount":296,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-title-1.jpg","keywords":["\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13","\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790"],"articleSection":["\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"],"inLanguage":"ja","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/","url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/","name":"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de - Learning at the Bench","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-title-1.jpg","datePublished":"2020-03-03T15:00:00+00:00","dateModified":"2025-05-19T03:27:09+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d"},"breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/#breadcrumb"},"inLanguage":"ja","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/"]}]},{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-title-1.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-title-1.jpg","width":1200,"height":630,"caption":"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de"},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/"},{"@type":"ListItem","position":2,"name":"BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/","name":"Learning at the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"ja"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d","name":"LATB Staff","image":{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","caption":"LATB Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/latbstaff\/"}]}},"taxonomy_info":{"category":[{"value":156,"label":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"}],"post_tag":[{"value":237,"label":"\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13"},{"value":21,"label":"\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-title-1-1024x538.jpg",760,399,true],"author_info":{"display_name":"LATB Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/latbstaff\/"},"comment_info":0,"category_info":[{"term_id":156,"name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","slug":"mol-bio","term_group":0,"term_taxonomy_id":156,"taxonomy":"category","description":"","parent":0,"count":528,"filter":"raw","meta":[],"cat_ID":156,"category_count":528,"category_description":"","cat_name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","category_nicename":"mol-bio","category_parent":0}],"tag_info":[{"term_id":237,"name":"\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13","slug":"protein-experiments","term_group":0,"term_taxonomy_id":237,"taxonomy":"post_tag","description":"\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c\u30b7\u30ea\u30fc\u30ba","parent":0,"count":23,"filter":"raw","meta":[]},{"term_id":21,"name":"\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790","slug":"protein-analysis","term_group":0,"term_taxonomy_id":21,"taxonomy":"post_tag","description":"","parent":0,"count":33,"filter":"raw","meta":[]}],"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic4-title-1.jpg","jetpack-related-posts":[{"id":24502,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qubit-dna-bid-ts-mbr-25081\/","url_meta":{"origin":60,"position":0},"title":"Qubit \u30b7\u30ea\u30fc\u30ba Fluorometer\u306b\u3064\u3044\u3066","author":"LatB Staff","date":"2025\u5e7412\u670822\u65e5","format":false,"excerpt":"Invitrogen\u2122 Qubit\u2122\u30b7\u30ea\u30fc\u30ba Fluorometer\u306f\u3001\u5c02\u7528\u306e\u8a66\u85ac\u30ad\u30c3\u30c8\u3092\u7528\u3044\u308b\u3053\u3068\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-25076_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-25076_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-25076_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-25076_eyecatch.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-25076_eyecatch.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-25076_eyecatch.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":24552,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qubit-dna-bid-ts-mbr-26001\/","url_meta":{"origin":60,"position":1},"title":"Qubit Fluorometer\u306b\u3064\u3044\u3066 \uff5eQubit Assay Kit\u306e\u6e2c\u5b9a\u7bc4\u56f2\uff5e","author":"LatB Staff","date":"2026\u5e741\u67089\u65e5","format":false,"excerpt":"Invitrogen\u2122 Qubit\u2122 Fluorometer \u306f\u3001\u5c02\u7528\u306e\u8a66\u85ac\u30ad\u30c3\u30c8\u3092\u7528\u3044\u308b\u3053\u3068\u3067DN\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-26001_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-26001_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-26001_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-26001_eyecatch.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-26001_eyecatch.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/qubit-dna-bid-ts-mbr-26001_eyecatch.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":20124,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-gene-therapy-ts-r-25015\/","url_meta":{"origin":60,"position":2},"title":"\u3010\u7b2c1\u56de\u3011Exosome\uff08\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\uff0f\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09\u3068\u306f\uff1f","author":"LatB Staff","date":"2026\u5e744\u670815\u65e5","format":false,"excerpt":"Exosome\uff08\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\uff0f\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09\u306e\u7814\u7a76\u304c\u6ce8\u76ee\u3092\u96c6\u3081\u3066\u3044\u307e\u3059\u3002\u8208\u5473\u306f\u3042\u3063\u3066\u3082\u3001\u3069\u306e\u3088\u3046\u306b\u7814\u2026","rel":"","context":"\u305d\u306e\u4ed6","block_context":{"text":"\u305d\u306e\u4ed6","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/else\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/6fae859c8c1eacdaa39f3ae843ae1bda-scaled.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":24606,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/transfection-antibody-bid-ts-mbr-26004\/","url_meta":{"origin":60,"position":3},"title":"\u7d30\u80de\u5185\u30bf\u30f3\u30d1\u30af\u8cea\u9664\u53bb\u30b7\u30b9\u30c6\u30e0\uff08Trim-Away\u6cd5\uff09\u306e\u7d39\u4ecb\uff5e\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6a5f\u5668\u3092\u4f7f\u7528\u3057\u3066\uff5e","author":"LatB Staff","date":"2026\u5e742\u67084\u65e5","format":false,"excerpt":"2018\u5e74\u306e\u6587\u732e(1)\u3067\u7d30\u80de\u5185\u306e\u5185\u5728\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u76f4\u63a5\u5206\u89e3\u3059\u308b\u3001Trim-Away\u6cd5\u304c\u7d39\u4ecb\u3055\u308c\u307e\u3057\u305f\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/01\/transfection-antibody-bid-ts-mbr-26004_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/01\/transfection-antibody-bid-ts-mbr-26004_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/01\/transfection-antibody-bid-ts-mbr-26004_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/01\/transfection-antibody-bid-ts-mbr-26004_eyecatch.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":25247,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-gene-expression-ts-r-25018\/","url_meta":{"origin":60,"position":4},"title":"\u3010\u7b2c4\u56de\u3011&#8221;RNA cargo&#8221;\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\uff5emiRNA\u306e\u5b9a\u91cf\u89e3\u6790\uff5e","author":"LatB Staff","date":"2026\u5e745\u670829\u65e5","format":false,"excerpt":"\u524d\u56de\uff08\u3010\u7b2c3\u56de\u3011\u201cRNA cargo\u201d\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\uff09\u3067\u306f\u3001\u30a8\u30af\u30bd\u30bd\u30fc\u30e0 \uff08\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09 \u304b\u3089\u306e\u2026","rel":"","context":"\u5206\u6790\u30fb\u6e2c\u5b9a\u95a2\u9023","block_context":{"text":"\u5206\u6790\u30fb\u6e2c\u5b9a\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/analysis\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":22156,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/nucleic-acid-purification-pcr-bid-ts-mbr-25046\/","url_meta":{"origin":60,"position":5},"title":"\u86cd\u5149\u6838\u9178\u5b9a\u91cf\u306b\u304a\u3051\u308b\u6e29\u5ea6\u7ba1\u7406\u306e\u91cd\u8981\u6027","author":"LatB Staff","date":"2025\u5e747\u670824\u65e5","format":false,"excerpt":"\u86cd\u5149\u6838\u9178\u5b9a\u91cf\u306f\u3001DNA\u3084RNA\u306e\u6fc3\u5ea6\u3092\u9ad8\u611f\u5ea6\u304b\u3064\u6b63\u78ba\u306b\u6e2c\u5b9a\u3059\u308b\u305f\u3081\u306e\u91cd\u8981\u306a\u6280\u8853\u3067\u3059\u3002\u7279\u306b\u7814\u7a76\u3084\u8a3a\u65ad\u306e\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/07\/nucleic-acid-purification-pcr-bid-ts-mbr-25046_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/07\/nucleic-acid-purification-pcr-bid-ts-mbr-25046_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/07\/nucleic-acid-purification-pcr-bid-ts-mbr-25046_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/07\/nucleic-acid-purification-pcr-bid-ts-mbr-25046_eyecatch.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]}],"jetpack_sharing_enabled":true,"amp_enabled":true,"_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/60","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/users\/988"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/comments?post=60"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/60\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media\/2083"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media?parent=60"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/categories?post=60"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/tags?post=60"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/division?post=60"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}