{"id":602,"date":"2017-02-12T00:00:00","date_gmt":"2017-02-12T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/"},"modified":"2026-05-01T19:04:52","modified_gmt":"2026-05-01T10:04:52","slug":"qpcr-basic36","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/","title":{"rendered":"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081"},"content":{"rendered":"\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-ad2f72ca wp-block-group-is-layout-flex\">\n<p>*\u66f4\u65b0\u65e5\uff1a<\/p>\n\n\n<div class=\"wp-block-post-date__modified-date wp-block-post-date\"><time datetime=\"2026-05-01T19:04:52+09:00\">2026\u5e745\u67081\u65e5<\/time><\/div><\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08qPCR\uff09\u306f\u3001PCR\u3067\u5897\u3048\u305f\u7523\u7269\u3092\u201c\u5f8c\u304b\u3089\u201d\u78ba\u8a8d\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u5897\u5e45\u306e\u904e\u7a0b\u3092\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u3068\u3057\u3066\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306b\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3057\u3001\u5b9a\u91cf\u3067\u304d\u308b\u624b\u6cd5\u3067\u3059\u3002\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3084\u75c5\u539f\u4f53\u691c\u51fa\u3001NGS\u30c7\u30fc\u30bf\u306e\u78ba\u8a8d\u3001\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u958b\u767a\u306eQC\u306a\u3069\u3001\u7814\u7a76\u304b\u3089\u5fdc\u7528\u307e\u3067\u5e45\u5e83\u304f\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>Thermo Fisher Scientific \u3067\u306f\u3001QuantStudio\u2122 \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0\u3084 Applied Biosystems\u2122 TaqMan\u2122 \u30a2\u30c3\u30bb\u30a4\u306a\u3069\u3001qPCR\u306b\u5fc5\u8981\u306a\u88c5\u7f6e\u3084\u8a66\u85ac\u3092\u5e45\u5e83\u304f\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u3053\u306e\u8a18\u4e8b\u3067\u306f\u3001\u521d\u5fc3\u8005\u304c\u307e\u305a\u8ff7\u308f\u306a\u3044\u305f\u3081\u306b\u3001qPCR\u306e\u4ed5\u7d44\u307f\u3001\u5b9f\u9a13\u7cfb\u3092\u7acb\u3061\u4e0a\u3052\u308b\u306b\u3042\u305f\u3063\u3066\u8003\u616e\u3059\u308b\u3079\u304d\u70b9\u3084\u6ce8\u610f\u70b9\u3092\u3001\u57fa\u672c\u304b\u3089\u3084\u3055\u3057\u304f\u89e3\u8aac\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0pcr\u3068\u306f-pcr\u3092-\u898b\u306a\u304c\u3089-\u5b9a\u91cf\u3059\u308b\"><strong>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3068\u306f\uff1aPCR\u3092\u201c\u898b\u306a\u304c\u3089\u201d\u5b9a\u91cf\u3059\u308b<\/strong><\/h2>\n\n\n\n<p>\u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR\u306f\u3001\u5897\u5e45\u304c\u7d42\u308f\u3063\u305f\u5f8c\u306b\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306a\u3069\u3067\u7523\u7269\u3092\u78ba\u8a8d\u3059\u308b\u306e\u304c\u4e00\u822c\u7684\u3067\u3057\u305f\u3002\u4e00\u65b9\u3001qPCR\u3067\u306f\u53cd\u5fdc\u4e2d\u306b\u86cd\u5149\u3092\u6e2c\u5b9a\u3057\u3001\u30b5\u30a4\u30af\u30eb\u3054\u3068\u306e\u86cd\u5149\u5024\u3092\u8a18\u9332\u3057\u3001\u89e3\u6790\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p>qPCR\u306e\u89e3\u6790\u3067\u767b\u5834\u3059\u308b\u306e\u304c Ct\u5024 \u3067\u3059\u3002Ct\uff08Cycle threshold\uff09\u5024\u3068\u306f\u3001\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u304c\u8a2d\u5b9a\u3057\u305f\u95be\u5024\uff08Threshold\uff09\u3092\u8d85\u3048\u305f\u30b5\u30a4\u30af\u30eb\u6570\u3092\u6307\u3057\u307e\u3059\uff08\u56f31\uff09\u3002\u521d\u671f\u92f3\u578b\u91cf\u304c\u591a\u3044\u307b\u3069\u95be\u5024\u5230\u9054\u304c\u65e9\u304f\u306a\u308a\u3001Ct\u5024\u306f\u5c0f\u3055\u304f\u306a\u308a\u307e\u3059\u3002\u56f31\u306e\u4f8b\u3067\u306f\u3001\u30b5\u30f3\u30d7\u30ebA\u306e\u65b9\u304c\u30b5\u30f3\u30d7\u30ebB\u3068\u6bd4\u8f03\u3057\u3066\u521d\u671f\u92f3\u578b\u91cf\u304c\u591a\u3044\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002qPCR\u3067\u306f\u3001\u7d76\u5bfe\u5b9a\u91cf\u3084\u0394\u0394Ct\u6cd5\u306a\u3069\u8907\u6570\u306e\u89e3\u6790\u624b\u6cd5\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u3044\u305a\u308c\u3082Ct\u5024\u3092\u57fa\u6e96\u306b\u30c7\u30fc\u30bf\u3092\u89e3\u6790\u3057\u307e\u3059\u3002\u306a\u304a\u3001\u88c5\u7f6e\u3084\u89e3\u6790\u30bd\u30d5\u30c8\u306b\u3088\u3063\u3066Ct\u5024\u306e\u7b97\u51fa\u65b9\u6cd5\u3084\u89e3\u6790\u8a2d\u5b9a\u304c\u7570\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u6bd4\u8f03\u3059\u308b\u969b\u306f\u3001\u540c\u3058\u6761\u4ef6\u30fb\u540c\u3058\u89e3\u6790\u8a2d\u5b9a\u3067\u8a55\u4fa1\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"621\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2017\/02\/b692d8a1bc9b8d908532df0ce240837d-1024x621.jpg\" alt=\"\" class=\"wp-image-25210\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2017\/02\/b692d8a1bc9b8d908532df0ce240837d-1024x621.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2017\/02\/b692d8a1bc9b8d908532df0ce240837d-300x182.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2017\/02\/b692d8a1bc9b8d908532df0ce240837d-768x466.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2017\/02\/b692d8a1bc9b8d908532df0ce240837d-1536x932.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2017\/02\/b692d8a1bc9b8d908532df0ce240837d-2048x1243.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">\u56f31.\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u304a\u3051\u308bCt\uff08Cycle threshold\uff09\u5024\u3068\u306f<\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u86cd\u5149\u3067\u3069\u3046\u6e2c\u308b-\u4ee3\u8868\u7684\u306a2\u3064\u306e\u691c\u51fa\u30b1\u30df\u30b9\u30c8\u30ea\u30fc\"><strong>\u86cd\u5149\u3067\u3069\u3046\u6e2c\u308b\uff1f\u4ee3\u8868\u7684\u306a2\u3064\u306e\u691c\u51fa\u30b1\u30df\u30b9\u30c8\u30ea\u30fc<\/strong><\/h2>\n\n\n\n<p>qPCR\u3067\u306f\u3001PCR\u5897\u5e45\u3092\u300c\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u5897\u52a0\u300d\u3068\u3057\u3066\u691c\u51fa\u3057\u307e\u3059\u3002\u73fe\u5728\u5e83\u304f\u4f7f\u308f\u308c\u3066\u3044\u308b\u691c\u51fa\u30b1\u30df\u30b9\u30c8\u30ea\u30fc\u306f\u5927\u304d\u304f2\u7cfb\u7d71\u306b\u5206\u304b\u308c\u307e\u3059\u3002\u3069\u3061\u3089\u306e\u624b\u6cd5\u3067\u3082\u5b9a\u91cf\u306f\u53ef\u80fd\u3067\u3059\u304c\u3001\u7279\u7570\u6027\u30fb\u8a2d\u8a08\u306e\u624b\u9593\u30fb\u904b\u7528\u306e\u3057\u3084\u3059\u3055\u304c\u7570\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<h5 class=\"wp-block-heading\" id=\"h-1-\u4e8c\u672c\u9396dna\u7d50\u5408\u8272\u7d20\u6cd5-\u4f8b-sybr-green\">1\uff09\u4e8c\u672c\u9396DNA\u7d50\u5408\u8272\u7d20\u6cd5\uff08\u4f8b\uff1aSYBR\u2122 Green\uff09<\/h5>\n\n\n\n<p>SYBR\u2122 Green \u306e\u3088\u3046\u306a\u4e8c\u672c\u9396DNA\u7d50\u5408\u8272\u7d20\u304c\u3001\u4e8c\u672c\u9396DNA\uff08dsDNA\uff09\u306b\u7d50\u5408\u3057\u305f\u3068\u304d\u86cd\u5149\u3092\u767a\u3059\u308b\u3068\u3044\u3046\u6027\u8cea\u3092\u5229\u7528\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002PCR\u7523\u7269\u304c\u5897\u3048\u308b\u307b\u3069dsDNA\u91cf\u304c\u5897\u3048\u3001\u305d\u308c\u306b\u6bd4\u4f8b\u3057\u3066\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u304c\u5f37\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u53cd\u5fdc\u7cfb\u3068\u3057\u3066\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u304c\u3042\u308c\u3070\u69cb\u7bc9\u3067\u304d\u308b\u305f\u3081\u30b7\u30f3\u30d7\u30eb\u3067\u3059\u3002\u305f\u3060\u3057\u3001\u3053\u306e\u65b9\u6cd5\u3067\u306f\u4e8c\u672c\u9396DNA\u3067\u3042\u308c\u3070\u3001\u975e\u7279\u7570\u7684\u5897\u5e45\u7523\u7269\u3084\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u3082\u691c\u51fa\u3055\u308c\u308b\u305f\u3081\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3068\u691c\u8a3c\u304c\u91cd\u8981\u3067\u3059\u3002\u5b9f\u9a13\u5f8c\u306b\u306f\u5fc5\u305a\u89e3\u96e2\u66f2\u7dda\uff08Melt curve\uff09\u89e3\u6790\u3092\u884c\u3044\u3001\u5358\u4e00\u30d4\u30fc\u30af\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002\u8907\u6570\u30d4\u30fc\u30af\u304c\u898b\u3089\u308c\u308b\u5834\u5408\u306f\u3001\u975e\u7279\u7570\u7523\u7269\u304c\u6df7\u5728\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u6761\u4ef6\u306e\u6700\u9069\u5316\u3084\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u518d\u8a2d\u8a08\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<h5 class=\"wp-block-heading\" id=\"h-2-\u914d\u5217\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u6cd5-\u4f8b-taqman-assay\">2\uff09\u914d\u5217\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u6cd5\uff08\u4f8b\uff1aTaqMan\u2122 Assay\uff09<\/h5>\n\n\n\n<p>TaqMan\u2122\u30a2\u30c3\u30bb\u30a4\u306f\u3001\u914d\u5217\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u3092\u7528\u3044\u308b\u691c\u51fa\u6cd5\u3067\u3059\u3002\u30d7\u30ed\u30fc\u30d6\u306f\u30ec\u30dd\u30fc\u30bf\u30fc\u8272\u7d20\u3068\u30af\u30a8\u30f3\u30c1\u30e3\u30fc\u3092\u4e21\u7aef\u306b\u6301\u3061\u3001\u5897\u5e45\u524d\u306f\u30af\u30a8\u30f3\u30c1\u30e3\u30fc\u306b\u3088\u308a\u86cd\u5149\u304c\u6291\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002PCR\u4f38\u9577\u53cd\u5fdc\u4e2d\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u30d7\u30ed\u30fc\u30d6\u306b\u5230\u9054\u3059\u308b\u3068\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u6301\u30645\u2018\u21923\u2032\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u306b\u3088\u308a\u30d7\u30ed\u30fc\u30d6\u304c\u5206\u89e3\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u30ec\u30dd\u30fc\u30bf\u30fc\u3068\u30af\u30a8\u30f3\u30c1\u30e3\u30fc\u304c\u5206\u96e2\u3057\u3001\u86cd\u5149\u304c\u691c\u51fa\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002\u30d7\u30ed\u30fc\u30d6\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306b\u7d50\u5408\u3057\u3066\u3044\u306a\u3051\u308c\u3070\u86cd\u5149\u306f\u751f\u3058\u306a\u3044\u3053\u3068\u304b\u3089\u3001SYBR\u2122 Green\u306a\u3069\u3088\u308a\u3082\u7279\u7570\u6027\u304c\u9ad8\u3044\u65b9\u6cd5\u3067\u3059\u3002<\/p>\n\n\n\n<p>\u8a2d\u8a08\u6e08\u307f\u306e TaqMan\u2122 Gene Expression Assay \u3084 TaqMan\u2122 SNP Genotyping Assay \u306a\u3069\u3092\u5229\u7528\u3059\u308c\u3070\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30fb\u30d7\u30ed\u30fc\u30d6\u8a2d\u8a08\u3084\u6700\u9069\u5316\u306e\u624b\u9593\u3092\u5927\u304d\u304f\u524a\u6e1b\u3067\u304d\u307e\u3059\u3002\u7279\u306b\u521d\u5fc3\u8005\u3084\u3001\u518d\u73fe\u6027\u3092\u91cd\u8996\u3059\u308b\u5b9f\u9a13\u3067\u306f\u5927\u304d\u306a\u5229\u70b9\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<h5 class=\"wp-block-heading\" id=\"h-\u3069\u3061\u3089\u3092\u9078\u3076\u3079\u304d\u304b\">\u3069\u3061\u3089\u3092\u9078\u3076\u3079\u304d\u304b\uff1f<\/h5>\n\n\n\n<p>\u6700\u7d42\u7684\u306a\u9078\u629e\u306f\u3001\u300c\u4f55\u3092\u91cd\u8996\u3059\u308b\u304b\u300d\u306b\u3088\u3063\u3066\u6c7a\u307e\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u7279\u7570\u6027\u30fb\u518d\u73fe\u6027\u30fb\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u5bfe\u5fdc\u3092\u91cd\u8996\u3059\u308b\u306a\u3089 TaqMan\u2122<\/li>\n\n\n\n<li>\u5358\u4e00\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u30b7\u30f3\u30d7\u30eb\u306b\u6e2c\u308b\u306a\u3089 SYBR\u2122<\/li>\n<\/ul>\n\n\n\n<p>\u521d\u5fc3\u8005\u306e\u5834\u5408\u3001\u300c\u8a66\u85ac\u304c\u5c11\u306a\u305d\u3046\u3060\u304b\u3089SYBR\u300d\u3068\u3044\u3046\u7406\u7531\u3060\u3051\u3067\u9078\u3076\u3068\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u691c\u8a3c\u306b\u6642\u9593\u3092\u53d6\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u30a2\u30c3\u30bb\u30a4\u8a2d\u8a08\u306e\u624b\u9593\u307e\u3067\u542b\u3081\u3066\u8003\u3048\u308b\u3068\u3001\u30c8\u30fc\u30bf\u30eb\u3067\u306f\u30d7\u30ed\u30fc\u30d6\u6cd5\u306e\u65b9\u304c\u30b9\u30e0\u30fc\u30ba\u306b\u7acb\u3061\u4e0a\u304c\u308b\u30b1\u30fc\u30b9\u3082\u5c11\u306a\u304f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u9006\u8ee2\u5199\u306e\u30b9\u30c6\u30c3\u30d7-1-step-\u3068-2-step-rt-qpcr\u306e\u8003\u3048\u65b9\"><strong>\u9006\u8ee2\u5199\u306e\u30b9\u30c6\u30c3\u30d7\uff1a1-Step \u3068 2-Step RT-qPCR\u306e\u8003\u3048\u65b9<\/strong><\/h2>\n\n\n\n<p>RNA\u3092\u5b9a\u91cf\u3059\u308b\u5834\u5408\u306f\u3001\u307e\u305a\u9006\u8ee2\u5199\uff08Reverse Transcription\uff1aRT\uff09\u3067cDNA\u3092\u4f5c\u3063\u3066\u304b\u3089qPCR\u3092\u884c\u3044\u307e\u3059\u3002RT\u3068qPCR\u3092\u5225\u3005\u306b\u5b9f\u65bd\u3059\u308b2-Step RT-qPCR\u3068RT\u3068qPCR\u3092\u9023\u7d9a\u7684\u306b\u884c\u30461-Step RT-qPCR\u304c\u3042\u308a\u307e\u3059\u3002<br>2-Step RT-qPCR \u306f\u3001\u6700\u521d\u306bRT\u3067cDNA\u3092\u4f5c\u308a\u3001\u5225\u53cd\u5fdc\u3067qPCR\u3057\u307e\u3059\u3002cDNA\u3092\u4fdd\u5b58\u3057\u3066\u304a\u3051\u308b\u305f\u3081\u3001\u8907\u6570\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u5c55\u958b\u3057\u3084\u3059\u304f\u3001\u67d4\u8edf\u6027\u304c\u9ad8\u3044\u306e\u304c\u5229\u70b9\u3067\u3059\u3002<\/p>\n\n\n\n<p>1-Step RT-qPCR \u306f\u3001RT\u3068qPCR\u3092\u540c\u4e00\u30c1\u30e5\u30fc\u30d6\u3067\u9023\u7d9a\u3057\u3066\u884c\u3046\u305f\u3081\u3001\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u304c\u7c21\u5358\u3067\u53d6\u308a\u6271\u3044\u306b\u4f34\u3046\u30b3\u30f3\u30bf\u30df\u30ea\u30b9\u30af\u3092\u6e1b\u3089\u3057\u3084\u3059\u3044\u306e\u304c\u5229\u70b9\u3067\u3059\u3002<\/p>\n\n\n\n<p>\u76ee\u7684\u3084\u904b\u7528\u30b9\u30bf\u30a4\u30eb\u306b\u5408\u308f\u305b\u3066\u9078\u629e\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-master-mix\u3068\u306f-qpcr\u53cd\u5fdc\u306b\u5fc5\u8981\u306a\u8a66\u85ac\u306e\u30df\u30af\u30b9\u30c1\u30e3\u30fc\"><strong>Master Mix\u3068\u306f\uff1fqPCR\u53cd\u5fdc\u306b\u5fc5\u8981\u306a\u8a66\u85ac\u306e\u30df\u30af\u30b9\u30c1\u30e3\u30fc<\/strong><\/h2>\n\n\n\n<p>qPCR\u3067\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3084\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u4ee5\u5916\u306b\u3082\u3001\u5897\u5e45\u53cd\u5fdc\u306b\u5fc5\u8981\u306a\u8907\u6570\u306e\u6210\u5206\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u305d\u308c\u3089\u3092\u3042\u3089\u304b\u3058\u3081\u6700\u9069\u306a\u6bd4\u7387\u3067\u6df7\u5408\u3057\u305f\u3082\u306e\u304c\u3001qPCR Master Mix\u3067\u3059\u3002Master Mix \u306b\u306f\u4e00\u822c\u7684\u306b\u3001\u6b21\u306e\u3088\u3046\u306a\u6210\u5206\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u8010\u71b1\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc<\/li>\n\n\n\n<li>dNTP<\/li>\n\n\n\n<li>Mg\u00b2\u207a\uff08\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\uff09<\/li>\n\n\n\n<li>\u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fc<\/li>\n<\/ul>\n\n\n\n<p>\u3053\u308c\u3089\u306fPCR\u304c\u6b63\u3057\u304f\u9032\u884c\u3059\u308b\u305f\u3081\u306b\u4e0d\u53ef\u6b20\u306a\u69cb\u6210\u8981\u7d20\u3067\u3059\u3002\u305f\u3068\u3048\u3070\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u65b0\u3057\u3044DNA\u3092\u5408\u6210\u3059\u308b\u9175\u7d20\u3067\u3042\u308a\u3001dNTP\u306f\u305d\u306e\u6750\u6599\u3068\u306a\u308a\u307e\u3059\u3002Mg\u00b2\u207a\u306f\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u6d3b\u6027\u306b\u5fc5\u9808\u306e\u88dc\u56e0\u5b50\u3067\u3042\u308a\u3001\u6fc3\u5ea6\u304c\u4e0d\u9069\u5207\u3060\u3068\u5897\u5e45\u52b9\u7387\u3084\u7279\u7570\u6027\u306b\u5f71\u97ff\u3057\u307e\u3059\u3002\u4ee5\u524d\u306f\u5404\u6210\u5206\u3092\u500b\u5225\u306b\u6df7\u5408\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3057\u305f\u304c\u3001\u73fe\u5728\u3067\u306f \u6700\u9069\u5316\u6e08\u307f\u306eMaster Mix\u3092\u4f7f\u7528\u3059\u308b\u306e\u304c\u4e00\u822c\u7684\u3067\u3059\u3002Thermo Fisher Scientific \u3067\u3082\u3001SYBR\u2122 Green \u7528\u3084 TaqMan\u2122 \u7528\u306a\u3069\u3001\u7528\u9014\u306b\u5fdc\u3058\u305f \u591a\u69d8\u306aqPCR Master Mix \u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002Master Mix\u3092\u4f7f\u3046\u30e1\u30ea\u30c3\u30c8\u306b\u306f\u3001\u6210\u5206\u30d0\u30e9\u30f3\u30b9\u304c\u6700\u9069\u5316\u3055\u308c\u3066\u3044\u308b\u305f\u3081\u518d\u73fe\u6027\u304c\u9ad8\u3044\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u304c\u6e1b\u308a\u30d2\u30e5\u30fc\u30de\u30f3\u30a8\u30e9\u30fc\u3084\u30b3\u30f3\u30bf\u30df\u30ea\u30b9\u30af\u3092\u4f4e\u6e1b\u3067\u304d\u308b\u3068\u3044\u3046\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30d7\u30e9\u30a4\u30de\u30fc-\u30d7\u30ed\u30fc\u30d6\u8a2d\u8a08\"><strong>\u30d7\u30e9\u30a4\u30de\u30fc\u30fb\u30d7\u30ed\u30fc\u30d6\u8a2d\u8a08<\/strong><\/h2>\n\n\n\n<p>qPCR\u3067\u3064\u307e\u305a\u304d\u3084\u3059\u3044\u306e\u304c\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3067\u3059\u3002<\/p>\n\n\n\n<p>\u8a2d\u8a08\u306e\u30dd\u30a4\u30f3\u30c8\u304c\u3044\u304f\u3064\u304b\u3042\u308a\u307e\u3059\u3002\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306f\u77ed\u3081\uff08\u76ee\u5b89 50\u2013150 bp \u7a0b\u5ea6\uff09\u306b\u3057\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306f18\u201324 nt\u7a0b\u5ea6\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u540c\u58eb\u306eTm\u5024\u306f\u8fd1\u3065\u3051\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u3084\u81ea\u5df1\u76f8\u88dc\u6027\u3092\u907f\u3051\u3001SYBR\u2122 Green\u904b\u7528\u3067\u306f\u7279\u306b\u3001\u89e3\u96e2\u66f2\u7dda\u3067\u5358\u4e00\u7523\u7269\u3092\u78ba\u8a8d\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\u307e\u305f\u3001RT-qPCR\u3067mRNA\u3092\u5bfe\u8c61\u306b\u3059\u308b\u5834\u5408\u306f\u3001\u53ef\u80fd\u306a\u3089\u30a8\u30af\u30bd\u30f3\u2013\u30a8\u30af\u30bd\u30f3\u5883\u754c\u3092\u307e\u305f\u3050\u8a2d\u8a08\u3067\u30b2\u30ce\u30e0DNA\u7531\u6765\u5897\u5e45\u3092\u6291\u3048\u307e\u3059\u3002<\/p>\n\n\n\n<h5 class=\"wp-block-heading\" id=\"h-\u4f7f\u3044\u3084\u3059\u3044\u30aa\u30f3\u30e9\u30a4\u30f3-\u30c4\u30fc\u30eb\u4f8b-\u5177\u4f53\u4f8b\">\u4f7f\u3044\u3084\u3059\u3044\u30aa\u30f3\u30e9\u30a4\u30f3\uff0f\u30c4\u30fc\u30eb\u4f8b\uff08\u5177\u4f53\u4f8b\uff09<\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Thermo Fisher\uff1aPrimer Express\u2122 \/ Primer Designer\uff08Applied Biosystems\u2122\u7cfb\u306e\u8a2d\u8a08\u30c4\u30fc\u30eb\uff09<\/li>\n\n\n\n<li>NCBI Primer-BLAST\uff08\u8a2d\u8a08\uff0b\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u78ba\u8a8d\u3092\u4e00\u4f53\u3067\u5b9f\u65bd\uff09<\/li>\n\n\n\n<li>Primer3\uff08\u6c4e\u7528\u30fb\u81ea\u7531\u5ea6\u306e\u9ad8\u3044\u8a2d\u8a08\u30c4\u30fc\u30eb\uff09<\/li>\n<\/ul>\n\n\n\n<p>\u8a2d\u8a08\u304c\u96e3\u3057\u3044\u3068\u611f\u3058\u308b\u65b9\u306b\u306f\u3001\u8a2d\u8a08\u6e08\u307f\u306e\u65e2\u88fd\u54c1TaqMan Assay\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002Applied Biosystems\u2122 TaqMan\u2122 Assay \u88fd\u54c1\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u3068\u3057\u3066\u3001\u5b9f\u8a3c\u3055\u308c\u305f\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u3092\u4f7f\u7528\u3057\u3066\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3001\u4e16\u754c\u4e2d\u306e\u7814\u7a76\u8005\u304b\u3089\u4fe1\u983c\u3092\u5f97\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb-\u6700\u4f4e\u9650\u3053\u308c\u3060\u3051\u306f\u5165\u308c\u3088\u3046\"><strong>\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff1a\u6700\u4f4e\u9650\u3053\u308c\u3060\u3051\u306f\u5165\u308c\u3088\u3046<\/strong><\/h2>\n\n\n\n<p>qPCR\u306f\u201c\u6e2c\u308c\u305f\u3088\u3046\u306b\u898b\u3048\u308b\u201d\u3053\u3068\u304c\u3042\u308b\u5206\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u304c\u91cd\u8981\u3067\u3059\u3002\u307e\u305a\u306f\u6b21\u306e\u57fa\u672c\u30bb\u30c3\u30c8\u304b\u3089\u59cb\u3081\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>NTC\uff08No Template Control\uff09\uff1a\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3092\u6dfb\u52a0\u3057\u306a\u3044\u53cd\u5fdc\u7cfb\u3067\u3059\u3002\u8a66\u85ac\u3078\u306e\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u3084\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u691c\u51fa\u306b\u5f79\u7acb\u3061\u307e\u3059\u3002<\/li>\n\n\n\n<li>NRT\uff08No RT Control\uff09\uff1a\u9006\u8ee2\u5199\u9175\u7d20\u3092\u6dfb\u52a0\u305b\u305a\u306b\u9006\u8ee2\u5199\u53cd\u5fdc\u3092\u884c\u3063\u305f\u30b5\u30f3\u30d7\u30eb\u3067\u3059\u3002RT-qPCR\u3067\u30b2\u30ce\u30e0DNA\u6df7\u5165\u3092\u78ba\u8a8d\u3057\u305f\u3044\u3068\u304d\u306b\u6e2c\u5b9a\u3057\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u691c\u91cf\u7dda\u30b5\u30f3\u30d7\u30eb\uff08Standard curve\uff09\uff1a\u7d76\u5bfe\u5b9a\u91cf\u3084PCR\u306e\u5897\u5e45\u52b9\u7387\u8a55\u4fa1\u306b\u4f7f\u7528\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u9069\u5207\u306a\u5b9f\u9a13\u30c6\u30af\u30cb\u30c3\u30af\"><strong>\u9069\u5207\u306a\u5b9f\u9a13\u30c6\u30af\u30cb\u30c3\u30af<\/strong><\/h2>\n\n\n\n<p>\u9069\u5207\u306a\u5b9f\u9a13\u30c6\u30af\u30cb\u30c3\u30af\u306e\u91cd\u8981\u6027\u3092\u904e\u5c0f\u8a55\u4fa1\u3057\u3066\u306f\u306a\u308a\u307e\u305b\u3093\u3002\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u8abf\u88fd\u304b\u3089PCR\u5f8c\u306e\u89e3\u6790\u306b\u81f3\u308b\u53cd\u5fdc\u306e\u5404\u30b9\u30c6\u30c3\u30d7\u3067\u3001\u5c02\u7528\u306e\u5b9f\u9a13\u5668\u5177\u304a\u3088\u3073\u6eb6\u6db2\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u6700\u5584\u3067\u3059\u3002\u30d5\u30a3\u30eb\u30bf\u30fc\u30c1\u30c3\u30d7\u304a\u3088\u3073\u30b9\u30af\u30ea\u30e5\u30fc\u5f0f\u306e\u30ad\u30e3\u30c3\u30d7\u306e\u30c1\u30e5\u30fc\u30d6\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30b3\u30f3\u30bf\u30df\u3092\u4f4e\u6e1b\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002qPCR\u306f\u9ad8\u611f\u5ea6\u306a\u5b9f\u9a13\u306e\u305f\u3081\u3001\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u306b\u306f\u7279\u306b\u6ce8\u610f\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9-\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0pcr\u30cf\u30f3\u30c9\u30d6\u30c3\u30af-\u6700\u65b0\u7248\">\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\uff1c\u6700\u65b0\u7248\uff1e<\/h3>\n\n\n\n<p>\u3053\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7406\u8ad6\u3084\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u306e\u8a2d\u8a08\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u77e5\u8b58\u304c\u63b2\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u59cb\u3081\u305f\u3070\u304b\u308a\u306e\u65b9\u3084\u3053\u308c\u304b\u3089\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/real-time-pcr-handbook-form.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr42_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u7121\u6599\u516c\u958b\u4e2d-\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0pcr\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\">\u3010\u7121\u6599\u516c\u958b\u4e2d\u3011\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0<\/h3>\n\n\n\n<p>\u7570\u5e38\u306aS\u5b57\u72b6\u306e\u5897\u5e45\u66f2\u7dda\u3001NTC\u3067\u306e\u5897\u5e45\u691c\u51fa\u3001\u5897\u5e45\u304c\u898b\u3089\u308c\u306a\u3044\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u5b9f\u9a13\u4e2d\u306b\u56f0\u3063\u305f\u969b\u306e\u624b\u52a9\u3051\u306b\u306a\u308b\u60c5\u5831\u3092\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/pcr-system-troubleshooting.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr42_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306f\u3001\u5f93\u6765\u306ePCR\u6280\u8853\u306e\u30d0\u30ea\u30a8\u30fc\u30b7\u30e7\u30f3\u306e\u4e00\u3064\u3067\u3042\u308a\u3001\u4e00\u822c\u306b\u30b5\u30f3\u30d7\u30eb\u4e2d\u306eDNA\u307e\u305f\u306fRNA\u306e\u5b9a\u91cf\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002PCR\u30b5\u30a4\u30af\u30eb\u4e2d\u306e\u5404\u30b9\u30c6\u30fc\u30b8\u306b\u304a\u3044\u3066\u751f\u6210\u3055\u308c\u308b\u5897\u5e45\u7523\u7269\u306e\u91cf\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u5b9a\u91cf\u3067\u304d\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":988,"featured_media":3016,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[7],"division":[],"class_list":{"0":"post-602","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-qpcr","9":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081 - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081\" \/>\n<meta property=\"og:description\" content=\"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306f\u3001\u5f93\u6765\u306ePCR\u6280\u8853\u306e\u30d0\u30ea\u30a8\u30fc\u30b7\u30e7\u30f3\u306e\u4e00\u3064\u3067\u3042\u308a\u3001\u4e00\u822c\u306b\u30b5\u30f3\u30d7\u30eb\u4e2d\u306eDNA\u307e\u305f\u306fRNA\u306e\u5b9a\u91cf\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002PCR\u30b5\u30a4\u30af\u30eb\u4e2d\u306e\u5404\u30b9\u30c6\u30fc\u30b8\u306b\u304a\u3044\u3066\u751f\u6210\u3055\u308c\u308b\u5897\u5e45\u7523\u7269\u306e\u91cf\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u5b9a\u91cf\u3067\u304d\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/\" \/>\n<meta property=\"og:site_name\" content=\"Learning at the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/ThermoFisherJapan\" \/>\n<meta property=\"article:published_time\" content=\"2017-02-12T00:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-05-01T10:04:52+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic36-title.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"630\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"LATB Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:site\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"LATB Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"11\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/\"},\"author\":{\"name\":\"LATB Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\"},\"headline\":\"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081\",\"datePublished\":\"2017-02-12T00:00:00+00:00\",\"dateModified\":\"2026-05-01T10:04:52+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/\"},\"wordCount\":182,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/qpcr-basic36-title.jpg\",\"keywords\":[\"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\"],\"articleSection\":[\"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023\"],\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/\",\"name\":\"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081 - Learning at the Bench\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/qpcr-basic36-title.jpg\",\"datePublished\":\"2017-02-12T00:00:00+00:00\",\"dateModified\":\"2026-05-01T10:04:52+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/#breadcrumb\"},\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/qpcr-basic36-title.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/qpcr-basic36-title.jpg\",\"width\":1200,\"height\":630,\"caption\":\"qpcr-basic36-title\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic36\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\",\"name\":\"Learning at the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"ja\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\",\"name\":\"LATB Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"caption\":\"LATB Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/latbstaff\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081 - Learning at the Bench","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/","og_locale":"ja_JP","og_type":"article","og_title":"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081","og_description":"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306f\u3001\u5f93\u6765\u306ePCR\u6280\u8853\u306e\u30d0\u30ea\u30a8\u30fc\u30b7\u30e7\u30f3\u306e\u4e00\u3064\u3067\u3042\u308a\u3001\u4e00\u822c\u306b\u30b5\u30f3\u30d7\u30eb\u4e2d\u306eDNA\u307e\u305f\u306fRNA\u306e\u5b9a\u91cf\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002PCR\u30b5\u30a4\u30af\u30eb\u4e2d\u306e\u5404\u30b9\u30c6\u30fc\u30b8\u306b\u304a\u3044\u3066\u751f\u6210\u3055\u308c\u308b\u5897\u5e45\u7523\u7269\u306e\u91cf\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u5b9a\u91cf\u3067\u304d\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002","og_url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/","og_site_name":"Learning at the Bench","article_publisher":"https:\/\/www.facebook.com\/ThermoFisherJapan","article_published_time":"2017-02-12T00:00:00+00:00","article_modified_time":"2026-05-01T10:04:52+00:00","og_image":[{"width":1200,"height":630,"url":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic36-title.jpg","type":"image\/jpeg"}],"author":"LATB Staff","twitter_card":"summary_large_image","twitter_creator":"@ThermoFisherJP","twitter_site":"@ThermoFisherJP","twitter_misc":{"\u57f7\u7b46\u8005":"LATB Staff","\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593":"11\u5206"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/"},"author":{"name":"LATB Staff","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d"},"headline":"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081","datePublished":"2017-02-12T00:00:00+00:00","dateModified":"2026-05-01T10:04:52+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/"},"wordCount":182,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic36-title.jpg","keywords":["\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR"],"articleSection":["\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"],"inLanguage":"ja","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/","url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/","name":"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081 - Learning at the Bench","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic36-title.jpg","datePublished":"2017-02-12T00:00:00+00:00","dateModified":"2026-05-01T10:04:52+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d"},"breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/#breadcrumb"},"inLanguage":"ja","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/"]}]},{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic36-title.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic36-title.jpg","width":1200,"height":630,"caption":"qpcr-basic36-title"},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic36\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/"},{"@type":"ListItem","position":2,"name":"\u307e\u305a\u306f\u30b3\u30b3\u304b\u3089\u59cb\u3081\u3088\u3046\uff01\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u6982\u8981\u3068\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u69cb\u6210\u8981\u7d20\u307e\u3068\u3081"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/","name":"Learning at the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"ja"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d","name":"LATB Staff","image":{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","caption":"LATB Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/latbstaff\/"}]}},"taxonomy_info":{"category":[{"value":156,"label":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"}],"post_tag":[{"value":7,"label":"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic36-title-1024x538.jpg",760,399,true],"author_info":{"display_name":"LATB Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/latbstaff\/"},"comment_info":0,"category_info":[{"term_id":156,"name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","slug":"mol-bio","term_group":0,"term_taxonomy_id":156,"taxonomy":"category","description":"","parent":0,"count":538,"filter":"raw","meta":[],"cat_ID":156,"category_count":538,"category_description":"","cat_name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","category_nicename":"mol-bio","category_parent":0}],"tag_info":[{"term_id":7,"name":"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR","slug":"qpcr","term_group":0,"term_taxonomy_id":7,"taxonomy":"post_tag","description":"","parent":0,"count":91,"filter":"raw","meta":[]}],"jetpack-related-posts":[{"id":23665,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-dna-gsd-ts-tr-25064\/","url_meta":{"origin":602,"position":0},"title":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011PCR\u306e\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3092\u601d\u3044\u5207\u308a\u632f\u3063\u3066\u307f\u305f","author":"LatB Staff","date":"2025\u5e7410\u670828\u65e5","format":false,"excerpt":"\u65b0\u305f\u306aPCR\u691c\u51fa\u7cfb\u3092\u69cb\u7bc9\u3059\u308b\u5834\u5408\u3001\u4f55\u3088\u308a\u3082\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u914d\u5217\u304c\u91cd\u8981\u3068\u3044\u3046\u3053\u3068\u306f\u591a\u304f\u306e\u65b9\u304c\u8a8d\u8b58\u3057\u3066\u3044\u308b\u306e\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":24545,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digital-pcr-gene-expression-gsd-ts-sd-25082\/","url_meta":{"origin":602,"position":1},"title":"\u3010Absolute Q\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306e\u6d3b\u7528\u4f8b\u3011\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u306e\u5b9a\u91cf","author":"LatB Staff","date":"2026\u5e741\u670816\u65e5","format":false,"excerpt":"Applied Biosystems\u2122 QuantStudio\u2122 Absolute Q\u2122 \u30c7\u30b8\u30bf\u30ebP\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/digital-pcr-gene-expression-gsd-ts-sd-25082_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/digital-pcr-gene-expression-gsd-ts-sd-25082_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/digital-pcr-gene-expression-gsd-ts-sd-25082_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":25325,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-gene-expression-ts-r-25019\/","url_meta":{"origin":602,"position":2},"title":"\u3010\u7b2c5\u56de\u3011 &#8220;RNA cargo&#8221;\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\u300d\uff5emRNA\u306e\u5b9a\u91cf\u89e3\u6790\uff5e","author":"LatB Staff","date":"2026\u5e746\u670811\u65e5","format":false,"excerpt":"\u4eca\u56de\u306f\u3001\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\uff08\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09\u4e2d\u306emRNA\u306e\u5b9a\u91cf\u89e3\u6790\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002\u7b2c3\u56de\u3067\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\u304b\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":25247,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-gene-expression-ts-r-25018\/","url_meta":{"origin":602,"position":3},"title":"\u3010\u7b2c4\u56de\u3011&#8221;RNA cargo&#8221;\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\uff5emiRNA\u306e\u5b9a\u91cf\u89e3\u6790\uff5e","author":"LatB Staff","date":"2026\u5e745\u670829\u65e5","format":false,"excerpt":"\u524d\u56de\uff08\u3010\u7b2c3\u56de\u3011\u201cRNA cargo\u201d\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\uff09\u3067\u306f\u3001\u30a8\u30af\u30bd\u30bd\u30fc\u30e0 \uff08\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09 \u304b\u3089\u306e\u2026","rel":"","context":"\u5206\u6790\u30fb\u6e2c\u5b9a\u95a2\u9023","block_context":{"text":"\u5206\u6790\u30fb\u6e2c\u5b9a\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/analysis\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":23370,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-dna-gsd-ts-tr-25061\/","url_meta":{"origin":602,"position":4},"title":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011PCR\u5f8c\u306e\u4fdd\u5b58\u6e29\u5ea6\u306f4\u2103\u4e00\u629e\u3067\u3059\u304b\uff1f\u4ed6\u306e\u6e29\u5ea6\u3082\u8a66\u3057\u3066\u307f\u305f","author":"LatB Staff","date":"2025\u5e7410\u670816\u65e5","format":false,"excerpt":"PCR\uff08\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\uff09\u306f\u3001\u5206\u5b50\u751f\u7269\u5b66\u306e\u5b9f\u9a13\u306e\u6839\u5e79\u3092\u306a\u3059\u975e\u5e38\u306b\u91cd\u8981\u306a\u6280\u8853\u3067\u3059\u3002PCR\u3092\u884c\u3063\u305f\u5f8c\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25061_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25061_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25061_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25061_eyecatch.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":25565,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-antibody-ts-r-25068_\/","url_meta":{"origin":602,"position":5},"title":"\u3010\u7b2c8\u56de\u3011\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\u30b5\u30d6\u30bf\u30a4\u30d7\u306e\u5206\u753b\u6cd5 \uff1a \u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\uff0fqRT-PCR\uff0f\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306b\u9069\u3057\u305f\u5206\u753b\u65b9\u6cd5","author":"LatB Staff","date":"2026\u5e749\u670829\u65e5","format":false,"excerpt":"\u672c\u7a3f\u3067\u306f\u3001\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\u819c\u4e0a\u306e\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6a19\u7684\u3068\u3057\u305f\u514d\u75ab\u5438\u7740\u6cd5\u306b\u57fa\u3065\u304f\u5206\u753b\u624b\u6cd5\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002\u30a8\u30af\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"amp_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic36-title.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/602","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/users\/988"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/comments?post=602"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/602\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media\/3016"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media?parent=602"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/categories?post=602"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/tags?post=602"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/division?post=602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}