{"id":6500,"date":"2020-05-18T16:35:33","date_gmt":"2020-05-18T07:35:33","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=6500"},"modified":"2021-12-01T16:48:25","modified_gmt":"2021-12-01T07:48:25","slug":"antibody_workshop_bid_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/antibody_workshop_bid_ts_1\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u7528\u6297\u4f53\u306e\u4fdd\u5b58\u65b9\u6cd5\u3092\u691c\u8a3c\u3057\u3066\u307f\u305f"},"content":{"rendered":"

\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30fc\u30bf\u30fc\u3092\u5229\u7528\u3057\u305f\u89e3\u6790\u306b\u304a\u3044\u3066\u3001\u4e00\u756a\u591a\u304f\u884c\u308f\u308c\u3066\u3044\u308b\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306f\u6297\u4f53\u3092\u4f7f\u7528\u3057\u305f\u89e3\u6790\u3067\u3059\u3002\u305d\u306e\u6297\u4f53\u306b\u306f\u86cd\u5149\u7269\u8cea\u304c\u7d50\u5408\u3057\u3066\u304a\u308a\u3001\u86cd\u5149\u7269\u8cea\u306e\u9055\u3044\u306b\u3088\u3063\u3066\u591a\u304f\u306e\u60c5\u5831\u3092\u7c21\u5358\u306b\u5165\u624b\u3067\u304d\u307e\u3059\u3002
\n\u305d\u3053\u3067\u4eca\u56de\u306f\u6297\u4f53\u306e\u4fdd\u5b58\u65b9\u6cd5\u306b\u3064\u3044\u3066\u691c\u8a3c\u3057\u3066\u307f\u307e\u3057\u305f\u3002\u4f55\u6c17\u306a\u304f\u884c\u3063\u3066\u3044\u308b\u6297\u4f53\u306e\u4fdd\u5b58\u306b\u3064\u3044\u3066\u6ce8\u610f\u3059\u3079\u304d\u70b9\u304c\u898b\u3048\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n

\u691c\u8a3c\u65b9\u6cd5<\/h2>\n

\u86cd\u5149\u6a19\u8b58\u6297\u4f53\u3092\u900f\u660e\u306a\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u306b\u5206\u6ce8\u3057\u3001\u5149\u306b\u5bfe\u3059\u308b\u5f71\u97ff\u3092\u78ba\u8a8d\u3059\u308b\u305f\u3081\u306b\u3001\u30c1\u30e5\u30fc\u30d6\u3092\u30a2\u30eb\u30df\u30db\u30a4\u30eb\u3067\u5305\u3093\u3060\u906e\u5149\uff08Dark\uff09\u6761\u4ef6\u3068\u3001\u86cd\u5149\u706f\u4e0b\uff08Light\uff09\u306b\u305d\u306e\u307e\u307e\u7f6e\u304f\u6761\u4ef6\u3067\u6bd4\u8f03\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u6e29\u5ea6\u306b\u5bfe\u3059\u308b\u5f71\u97ff\u3092\u78ba\u8a8d\u3059\u308b\u305f\u3081\u306b\u3001\u51b7\u8535\u5eab\uff08Cool\uff09\u5185\u306b\u4fdd\u5b58\u3059\u308b\u6761\u4ef6\u3068\u5ba4\u6e29\uff08RT\uff09\u3067\u3042\u308b\u30e9\u30dc\u5185\u306b\u7f6e\u304f\u6761\u4ef6\u3067\u6bd4\u8f03\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u30894\u3064\u306e\u6761\u4ef6\u3067\u3069\u306e\u7a0b\u5ea6\u3001\u86cd\u5149\u6a19\u8b58\u6297\u4f53\u306e\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u5f37\u5ea6\u306b\u5f71\u97ff\u304c\u3042\u308b\u304b\u30011\u65e5\u5f8c\u30687\u65e5\u5f8c\u306bInvitrogen\u2122 Attune\u2122 NxT Flow Cytometer\u3067\u6e2c\u5b9a\u3057\u78ba\u8a8d\u3057\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n

\u4fdd\u5b58\u6761\u4ef6
\n\u2460 \u51b7\u8535 \/ \u906e\u5149 (\u63a8\u5968\u6761\u4ef6)
\n\u2461 \u51b7\u8535 \/ \u86cd\u5149\u706f\u4e0b
\n\u2462 \u5ba4\u6e29 \/ \u906e\u5149
\n\u2463 \u5ba4\u6e29 \/\u86cd\u5149\u706f\u4e0b<\/p>\n

\u4f7f\u7528\u6297\u4f53
\n1. CD8-FITC
\n2. CD4-PE-Cy7<\/p>\n

\"\"<\/p>\n

\u4fdd\u5b58\u65b9\u6cd5\u306e\u691c\u8a3c\u7d50\u679c1 \uff5e FITC \uff5e<\/h2>\n

FITC\u6a19\u8b58\u6297\u4f53\u3067\u306f\u3001\u653e\u7f6e\u524dFITC\u30dd\u30b8\u30c6\u30a3\u30d6\u96c6\u56e3\u306e\u8f1d\u5ea6\uff08median\u5024\uff09\u3092100%\u3068\u3057\u305f\u5834\u5408\u30011\u9031\u9593\u5f8c\u306e\u906e\u5149\u6761\u4ef6\u3067\u306f\u3001\u6e29\u5ea6\u306b\u4f9d\u5b58\u3059\u308b\u3053\u3068\u306a\u304f\u307b\u3068\u3093\u3069\u5909\u5316\u304c\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3057\u304b\u3057\u2463 \u5ba4\u6e29 \/ \u86cd\u5149\u706f\u4e0b\u6761\u4ef6\u3067\u306f\u8f1d\u5ea6\u304c60%\u3001\u2461\u51b7\u8535 \/ \u86cd\u5149\u706f\u4e0b\u6761\u4ef6\u3067\u306f80%\u307e\u3067\u4f4e\u4e0b\u3057\u307e\u3057\u305f\u3002<\/p>\n

\"\"<\/p>\n

\u4fdd\u5b58\u65b9\u6cd5\u691c\u8a3c\u7d50\u679c2 \uff5e PE-Cy7 \uff5e<\/h2>\n

PE-Cy7\u6a19\u8b58\u6297\u4f53\u3067\u306f\u3001FITC\u6a19\u8b58\u6297\u4f53\u3068\u540c\u69d8\u306b1\u9031\u9593\u5f8c\u3067\u3082\u906e\u5149\u6761\u4ef6\u306b\u304a\u304d\u307e\u3057\u3066\u306f\u3001\u6e29\u5ea6\u306b\u4f9d\u5b58\u3059\u308b\u3053\u3068\u306a\u304f\u307b\u3068\u3093\u3069\u5909\u5316\u304c\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3057\u304b\u3057\u86cd\u5149\u706f\u4e0b\u6761\u4ef6\u3067\u306fFITC\u6a19\u8b58\u6297\u4f53\u306b\u6bd4\u3079\u3066\u5f71\u97ff\u304c\u5927\u304d\u304f\u3001\u7279\u306b\u2463 \u5ba4\u6e29 \/ \u86cd\u5149\u706f\u4e0b\u6761\u4ef6\u3067\u306f\u6e2c\u5b9a\u7d50\u679c\u306b\u5f71\u97ff\u304c\u51fa\u308b\u7a0b\u5ea6\u307e\u3067\u8f1d\u5ea6\u304c\u4e0b\u304c\u308a\u307e\u3057\u305f\u3002<\/p>\n

\"\"<\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u57fa\u672c\u7684\u306b\u306f\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u3084\u30e9\u30d9\u30eb\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u3001\u4fdd\u5b58\u6761\u4ef6\u306b\u5f93\u3063\u3066\u3044\u305f\u3060\u304f\u3053\u3068\u3092\u304a\u52e7\u3081\u3044\u305f\u3057\u307e\u3059\u3002\u4eca\u56de\u30012\u7a2e\u985e\u306e\u86cd\u5149\u6a19\u8b58\u6297\u4f53\u3092\u7528\u3044\u30664\u3064\u306e\u6761\u4ef6\u3067\u691c\u8a3c\u3057\u305f\u7d50\u679c\u3001\u5149\u306e\u66b4\u9732\u304c\u88fd\u54c1\u306e\u52a3\u5316\u3092\u65e9\u3081\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u7279\u306bPE-Cy7\u306e\u3088\u3046\u306a\u30bf\u30f3\u30c7\u30e0\u8272\u7d20\u306f\u3001\u5149\u3078\u306e\u611f\u53d7\u6027\u304c\u9ad8\u304f\u6ce8\u610f\u304c\u5fc5\u8981\u3067\u3059\u3002\u6297\u4f53\u3092\u9577\u671f\u306b\u4e98\u308a\u5b89\u5b9a\u7684\u306b\u3054\u4f7f\u7528\u3044\u305f\u3060\u304f\u305f\u3081\u306b\u306f\u3001\u4ee5\u4e0b\u306b\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30fb\u7d19\u88fd\u306e\u30b9\u30c8\u30ec\u30a4\u30b8\u30dc\u30c3\u30af\u30b9\u306a\u3069\u906e\u5149\u53ef\u80fd\u306a\u5bb9\u5668\u306b\u5165\u308c\u3066\u4fdd\u5b58\u3059\u308b
\n\u30fb\u5206\u6ce8\u4fdd\u5b58\u3059\u308b\u969b\u306b\u306f\u3001\u906e\u5149\u6027\u306e\u3042\u308b\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u3092\u4f7f\u7528\u3059\u308b
\n\u30fb\u4f7f\u7528\u6642\u3001\u3075\u305f\u3092\u958b\u3051\u308b\u6642\u9593\u3092\u3067\u304d\u308b\u3060\u3051\u77ed\u304f\u3059\u308b<\/p>\n

\u5f0a\u793e\u3067\u306f\u514d\u75ab\u5b66\u3001\u816b\u760d\u5b66\u306a\u3069\u306b\u7528\u3044\u3089\u308c\u308b\u30de\u30eb\u30c1\u30ab\u30e9\u30fc\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u7528\u306e\u86cd\u5149\u8272\u7d20\u30b3\u30f3\u30b8\u30e5\u30b2\u30fc\u30c8\u6297\u4f53\u304a\u3088\u3073\u95a2\u9023\u8a66\u85ac\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002
\nInvitrogen\u2122 eBioscience\u2122 \u88fd\u54c1\u3092\u542b\u3080\u591a\u304f\u306e\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u7528\u6297\u4f53\u306b\u306f\u3001\u30d0\u30a4\u30aa\u30ec\u30c3\u30c8\u30ec\u30fc\u30b6\u30fc\u3067\u52b1\u8d77\u53ef\u80fd\u306aSuper Bright\u8272\u7d20\u3092\u542b\u3080\u591a\u69d8\u306a\u8272\u7d20\u3092\u53d6\u308a\u305d\u308d\u3048\u3066\u3044\u307e\u3059\u3002
\n\u7686\u3055\u307e\u306e\u7814\u7a76\u306b\u5f79\u7acb\u3064\u6297\u4f53\u304c\u304d\u3063\u3068\u898b\u3064\u304b\u308b\u306f\u305a\u3067\u3059\u3002\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u7528\u6297\u4f53\u306e\u691c\u7d22\u306f\u3053\u3061\u3089\u00bb<\/a><\/p>\n

 <\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30fc\u30bf\u30fc\u3092\u5229\u7528\u3057\u305f\u89e3\u6790\u306b\u304a\u3044\u3066\u3001\u4e00\u756a\u591a\u304f\u884c\u308f\u308c\u3066\u3044\u308b\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306f\u6297\u4f53\u3092\u4f7f\u7528\u3057\u305f\u89e3\u6790\u3067\u3059\u3002\u305d\u306e\u6297\u4f53\u306b\u306f\u86cd\u5149\u7269\u8cea\u304c\u7d50\u5408\u3057\u3066\u304a\u308a\u3001\u86cd\u5149\u7269\u8cea\u306e\u9055\u3044\u306b\u3088\u3063\u3066\u591a\u304f\u306e\u60c5\u5831\u3092\u7c21\u5358\u306b\u5165\u624b\u3067\u304d\u307e\u3059\u3002 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