{"id":6562,"date":"2020-05-19T10:28:32","date_gmt":"2020-05-19T01:28:32","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=6562"},"modified":"2025-02-10T16:39:44","modified_gmt":"2025-02-10T07:39:44","slug":"virus_spa_bid_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/virus_spa_bid_ts_1\/","title":{"rendered":"\u30a6\u30a4\u30eb\u30b9\/\u7d30\u83cc\u7531\u6765\u306e\u6838\u9178\uff08DNA\/RNA\uff09\u7cbe\u88fd\u306b\u6c42\u3081\u3089\u308c\u308b\u3053\u3068 ~\u9ad8\u53ce\u91cf\/\u9ad8\u7d14\u5ea6\u3092\u8d85\u3048\u305f\u30b9\u30d4\u30fc\u30c9\u3068\u51e6\u7406\u80fd\u529b\u3092\u517c\u306d\u305d\u308d\u3048\u305fKingFisher\u2122\u30b7\u30ea\u30fc\u30ba\u306e\u7d39\u4ecb~"},"content":{"rendered":"

\u30a6\u30a4\u30eb\u30b9\u7531\u6765\u306e\u6838\u9178\uff08DNA\/RNA\uff09\u3092\u6a19\u7684\u3068\u3057\u305f\u691c\u67fb\u306e\u7cbe\u5ea6\u306f\u3001\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u306e\u7d14\u5ea6\u3001\u304a\u3088\u3073\u53cd\u5fdc\u306b\u6301\u3061\u8fbc\u3080\u6838\u9178\u91cf\u306b\u5927\u304d\u304f\u4f9d\u5b58\u3057\u307e\u3059\u3002\u7279\u306b\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u306e\u5224\u5b9a\u3067\u306f\u3001\u691c\u51fa\u9650\u754c\u306b\u8fd1\u3044\u30b3\u30d4\u30fc\u6570\u3057\u304b\u5b58\u5728\u3057\u306a\u3044\u30b5\u30f3\u30d7\u30eb\u3082\u591a\u304f\u5b58\u5728\u3059\u308b\u305f\u3081\u3001\u5fae\u91cf\u306a\u30a6\u30a4\u30eb\u30b9\u7531\u6765\u306e\u6838\u9178\u3092\u30ed\u30b9\u306a\u304f\u3001\u9ad8\u7d14\u5ea6\u3067\u56de\u53ce\u3067\u304d\u308b\u7cbe\u88fd\u30b7\u30b9\u30c6\u30e0\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u7b2c\u4e00\u6761\u4ef6\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u64cd\u4f5c\u306e\u624b\u6280\u306b\u3088\u308b\u3070\u3089\u3064\u304d\u3084\u30df\u30b9\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u3053\u3068\u3082\u91cd\u8981\u3067\u3059\u3002<\/p>\n

\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01
\n\u30fb\u611f\u67d3\u75c7 \/ \u30de\u30a4\u30af\u30ed\u30d0\u30a4\u30aa\u30fc\u30e0\u7814\u7a76
\n\u30fb\u73fe\u884c\u3088\u308a\u9ad8\u611f\u5ea6\u306a\u691c\u51fa\u304c\u5fc5\u8981
\n\u30fb1\u56de\u306e\u6838\u9178\u7cbe\u88fd\u306712\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u51e6\u7406<\/p>\n

\u3088\u308a\u9ad8\u611f\u5ea6\u306a\u691c\u51fa\u3092\u5b9f\u73fe\uff01<\/h2>\n

\u81ea\u52d5\u7cbe\u88fd\u3092\u30b5\u30dd\u30fc\u30c8\u3059\u308bKingFisher\u2122\u30b7\u30ea\u30fc\u30ba<\/h2>\n

\u81ea\u52d5\u7cbe\u88fd\u3092\u30b5\u30dd\u30fc\u30c8\u3059\u308bKingFisher\u2122\u30b7\u30ea\u30fc\u30ba\u3067\u306f\u3001\u78c1\u6027\u30d3\u30fc\u30ba\u3092\u30d9\u30fc\u30b9\u3068\u3057\u305f\u7cbe\u88fd\u30b7\u30b9\u30c6\u30e0\uff08MagMAX\u2122\u30b7\u30ea\u30fc\u30ba\uff09\u306b\u6700\u9069\u5316\u3055\u308c\u305f\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u304c\u4f5c\u6210\u3055\u308c\u3066\u304a\u308a\u3001\u9ad8\u52b9\u7387\u3067\u518d\u73fe\u6027\u306e\u9ad8\u3044\u6838\u9178\u7cbe\u88fd\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002MagMAX\u2122\u30b7\u30ea\u30fc\u30ba\u3067\u306f\u30b9\u30bf\u30fc\u30c8\u30b5\u30f3\u30d7\u30eb\u3084\u6a19\u7684\u6838\u9178\u306e\u30bf\u30a4\u30d7\u306b\u5408\u308f\u305b\u3066\u3055\u307e\u3056\u307e\u306aKit\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3059\u3079\u3066\u306eKit\u306f\u78c1\u6027\u30d3\u30fc\u30ba\u306e\u8868\u9762\u306b\u6838\u9178\u3092\u7d50\u5408\/\u6d17\u6d44\/\u6eb6\u51fa\u306e\u5de5\u7a0b\u3067\u7d4c\u3066\u56de\u53ce\u3092\u884c\u3044\u307e\u3059\u3002KingFisher\u2122\u30b7\u30ea\u30fc\u30ba\u306f\u3001\u78c1\u6027\u30d3\u30fc\u30ba\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30d8\u30c3\u30c9\u306b\u7d50\u5408\u3055\u305b\u305f\u72b6\u614b\u3067\u3001\u7d50\u5408\/\u6d17\u6d44\/\u6eb6\u51fabuffer\u306e\u5206\u6ce8\u3055\u308c\u305fwell(\u307e\u305f\u306fPlate)\u3092\u79fb\u52d5\u3059\u308b\u3053\u3068\u3067\u3001\u6838\u9178\u7cbe\u88fd\u3092\u884c\u3044\u307e\u3059\uff08\u56f3\uff11\uff09\u3002\u307e\u305f\u7cbe\u88fd\u5de5\u7a0b\u3067\u306f\u5b8c\u5168\u306b\u6700\u9069\u5316\u3055\u308c\u305f\u5f37\u5ea6\u306e\u30df\u30c3\u30af\u30b9\u51e6\u7406\u3084\u52a0\u71b1\u51e6\u7406\u3092\u7d44\u307f\u5408\u308f\u3055\u308b\u3053\u3068\u3067\u6700\u9ad8\u306e\u30d1\u30d5\u30a3\u30fc\u30de\u30f3\u30b9\u304c\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u307e\u3059\u3002KingFisher\u30b7\u30ea\u30fc\u30ba\u306f\u30cb\u30fc\u30ba\u306b\u5408\u3063\u305f\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u6027\u306e\u6a5f\u5668\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08\u8868\uff11\uff09
\n\"\"<\/p>\n

\"\"<\/p>\n

\u611f\u67d3\u75c7 \/ \u30de\u30a4\u30af\u30ed\u30d0\u30a4\u30aa\u30fc\u30e0\u7814\u7a76\u306b\u9069\u3057\u305fMagMAX\u2122\u30b7\u30ea\u30fc\u30ba<\/h2>\n

MagMAX\u2122\u30b7\u30ea\u30fc\u30ba\u306e\u88fd\u54c1\u30e9\u30a4\u30f3\u30a2\u30c3\u30d7<\/a>\u306b\u611f\u67d3\u75c7 \/ \u30de\u30a4\u30af\u30ed\u30d0\u30a4\u30aa\u30fc\u30e0\u7814\u7a76\u306b\u7279\u5316\u3057\u305f\u88fd\u54c1\uff08\u88682\uff09\u304c\u65b0\u305f\u306b\u8ffd\u52a0\u3055\u308c\u307e\u3057\u305f\u3002
\n1\u3011\u30a6\u30a4\u30eb\u30b9RNA\/DNA\u7cbe\u88fd\u306b\u30d5\u30a9\u30fc\u30ab\u30b9\u3057\u305fMagMAX\u2122 Viral\/Pathogen Nucleic Acid Isolation Kit<\/a>\u30012\u3011\u30a6\u30a4\u30eb\u30b9RNA\/DNA\u306b\u52a0\u3048\u3001\u3088\u308a\u6eb6\u89e3\u306e\u96e3\u3057\u3044\u30d0\u30af\u30c6\u30ea\u30a2\uff08\u30b0\u30e9\u30e0\u967d\u6027\/\u30b0\u30e9\u30e0\u9670\u6027\u83cc\uff09\u304b\u3089\u306eRNA\/DNA\u3082\u53ef\u80fd\u306aMagMAX\u2122 Viral\/Pathogen Ultra Nucleic Acid Isolation Kit<\/a>\u30013\u3011\u593e\u96d1\u7269\u306e\u591a\u3044\u7cde\u4fbf\u3084\u571f\u58cc\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u9ad8\u7d14\u5ea6\u306a\u30a6\u30a4\u30eb\u30b9\/\u30d0\u30af\u30c6\u30ea\u30a2\u7531\u6765\u306eRNA\/DNA\u306b\u6700\u9069\u5316\u3055\u308c\u305fMagMAX\u2122 Microbiome Ultra Nucleic Acid Isolation Kit<\/a>\u3067\u3059\u3002
\n\u3044\u305a\u308c\u306e\u30b7\u30b9\u30c6\u30e0\u3082\u7cbe\u88fd\u3055\u308c\u305fRNA\/DNA\u306f\u3001PCR, real-time PCR, microarray, next-generation sequencing\u306a\u3069\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u5229\u7528\u53ef\u80fd\u3067\u3059\u3002\u307e\u305fNGS\u89e3\u6790\u306b\u60aa\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u30ad\u30e3\u30ea\u30a2\u3092\u6dfb\u52a0\u305b\u305a\u306b\u7cbe\u88fd\u304c\u884c\u3048\u307e\u3059\u3002
\n\u53c2\u8003\u8cc7\u6599\uff1a\u30de\u30a4\u30af\u30ed\u30d0\u30a4\u30aa\u30fc\u30e0\u89e3\u6790<\/a><\/p>\n

\"\"<\/p>\n

\u30a6\u30a4\u30eb\u30b9RNA\/DNA\u306e\u9ad8\u611f\u5ea6\u306a\u691c\u51fa\u306b\uff01\u3000MagMAX Viral\/Pathogen Nucleic Acid Isolation Kit<\/h2>\n

\u30d2\u30c8\u4f53\u6db2\u306e\u30a6\u30a4\u30eb\u30b9\u306e\u691c\u51fa\u306f\u3001\u611f\u67d3\u75c7\u306e\u7814\u7a76\u3068\u7ba1\u7406\u306b\u4e0d\u53ef\u6b20\u3067\u3059\u3002
\n\u30a6\u30a4\u30eb\u30b9\u8ca0\u8377\u8a66\u9a13\uff08Viral load testing\uff09<\/a>\u306f\u3001\u6297\u30a6\u30a4\u30eb\u30b9\u7642\u6cd5\u306e\u6709\u52b9\u6027\u8a55\u4fa1\u3084\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u306e\u9032\u884c\u306e\u30e2\u30cb\u30bf\u30fc\u3001\u3055\u3089\u306b\u9ad8\u611f\u5ea6\u306a\u691c\u51fa\u304c\u53ef\u80fd\u3068\u306a\u308c\u3070\u521d\u671f\u6bb5\u968e\u3067\u611f\u67d3\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002
\nMagMAX\u2122 Viral\/Pathogen Nucleic Acid Isolation Kit<\/a>\u306f\u7279\u306b\u4f53\u6db2\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u306e\u30a6\u30a4\u30eb\u30b9RNA\/DNA\u56de\u53ce\u306b\u7279\u5316\u3055\u308c\u305fKit\u3067\u3001\u77ed\u6642\u9593\u3067\u4f53\u6db2\u4e2d\u306b\u5fae\u91cf\u306b\u5b58\u5728\u3059\u308b\u30a6\u30a4\u30eb\u30b9RNA\/DNA\u3092\u52b9\u7387\u7684\u306b\u56de\u53ce\u3067\u304d\u307e\u3059\u3002KingFisher\u2122 Flex\u3068\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u300196\u30b5\u30f3\u30d7\u30eb\u3092\u308f\u305a\u304b40\u5206\u7a0b\u5ea6\u3067\u51e6\u7406\u3067\u304d\u307e\u3059\u3002
\n\u5168\u8840\u3001\u8840\u6f3f\u3001\u5c3f\u30b5\u30f3\u30d7\u30eb\uff08400\u03bcL\uff09\u306b\u305d\u308c\u305e\u308c 50\uff5e1000\u30b3\u30d4\u30fc\u306ecytomegalovirus (CMV) \u304a\u3088\u3073 Epstein-Barr virus (EBV) \u30b9\u30d1\u30a4\u30af\u3057\u305f\u7cbe\u88fd\u8a66\u9a13\u3067\u306f\u3001\u7cbe\u88fd\u5f8c\u306e\u30b5\u30f3\u30d7\u30eb\uff08\u6eb6\u51fa\u91cf50\u03bcL\uff09\u304b\u30894\u03bcL\u3092\u5206\u53d6\u3057\u3066\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3067\u691c\u51fa\u3092\u884c\u3044\u611f\u5ea6\u306e\u30c6\u30b9\u30c8\u3092\u884c\u3063\u305f\u3068\u3053\u308d\u3001\u308f\u305a\u304b50\u30b3\u30d4\u30fc\u306e\u30b9\u30d1\u30a4\u30af\u30b5\u30f3\u30d7\u30eb\u3067\u3082\u5341\u5206\u306b\u691c\u51fa\u3067\u304d\u308b\u611f\u5ea6\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\uff08\u56f32\uff09\u3002
\n\u5b9f\u9a13\u306e\u8a73\u7d30\u306f\u3053\u3061\u3089\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/a><\/p>\n

\"\"\u307e\u305fdsDNA\u3060\u3051\u3067\u306a\u304f (+)ssRNA, (-)ssRNA\u30a6\u30a4\u30eb\u30b9\u3082\u540c\u69d8\u306b\u9ad8\u611f\u5ea6\u306a\u691c\u51fa\u304c\u53ef\u80fd\u3067\u3001\u4ed6\u793e\u306e\u30a6\u30a4\u30eb\u30b9\u7cbe\u88fd\u30b7\u30b9\u30c6\u30e0\u3068\u306e\u6bd4\u8f03\u30c6\u30b9\u30c8\u3067\u306f\u540c\u7b49\u4ee5\u4e0a\u306e\u611f\u5ea6\u3067\u30a6\u30a4\u30eb\u30b9\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\uff08\u56f33\uff09\u3002<\/p>\n

\"\"
\n\u5168\u4e16\u754c\u3067\u8105\u5a01\u3068\u306a\u3063\u3066\u3044\u308b \u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9SARS-CoV-2\uff08\u611f\u67d3\u75c7\u540d\uff1aCOVID-19\uff09\u306ePCR\u691c\u51fa\u306e\u305f\u3081\u306e\u691c\u4f53\u304b\u3089\u306e\u30a6\u30a4\u30eb\u30b9RNA\u7cbe\u88fd\u306b\u3053\u306eKit\u3092\u63d0\u6848\u3057\u3066\u3044\u307e\u3059\uff08\u8a73\u7d30<\/a>\uff09\u3002<\/p>\n

\u73fe\u5728\u3001\u9ad8\u53ce\u91cf\/\u9ad8\u7d14\u5ea6\u306e\u6838\u9178\u3092\u8fc5\u901f\u306b\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u3067\u51e6\u7406\u3067\u304d\u308b\u30b7\u30b9\u30c6\u30e0\u306e\u69cb\u7bc9\u304c\u591a\u304f\u306e\u73fe\u5834\u3067\u6c42\u3081\u3089\u308c\u3066\u3044\u307e\u3059\u3002
\n\u30a6\u30a4\u30eb\u30b9\u304a\u3088\u3073\u7d30\u83cc\u306b\u3088\u308b\u611f\u67d3\u75c7\u89e3\u6790 \/ \u30de\u30a4\u30af\u30ed\u30d0\u30a4\u30aa\u30fc\u30e0\u7814\u7a76\u7528\u306b\u7279\u5316\u3057\u305f\u7cbe\u88fdKit\u304a\u3088\u3073\u81ea\u52d5\u7cbe\u88fd\u30b7\u30b9\u30c6\u30e0\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u52b9\u7387\u7684\u306a\u7814\u7a76\u3092\u30b5\u30dd\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>\n

\u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9SARS-CoV-2\uff08\u611f\u67d3\u75c7\u540d\uff1aCOVID-19\uff09\u306e\u907a\u4f1d\u5b50\u89e3\u6790\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3<\/h2>\n

\u30a6\u30a4\u30eb\u30b9\u691c\u51fa\u306e\u305f\u3081\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u305c\u3072\u30a6\u30a4\u30eb\u30b9\u691c\u51fa\u7814\u7a76\u306b\u3054\u6d3b\u7528\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u8a73\u7d30\u3092\u898b\u308b<\/a><\/p>\n

\u81ea\u52d5\u5316\u30d7\u30ed\u30c8\u30b3\u30eb\u3068\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2<\/h2>\n

MagMAX \u6838\u9178\u7cbe\u88fd\u30ad\u30c3\u30c8\u7528\u3084\u3001Dynabead \u304a\u3088\u3073 Pierce \u306e\u30bf\u30f3\u30d1\u30af\u8cea\u7cbe\u88fd\u30d3\u30fc\u30ba\u304a\u3088\u3073\u30ad\u30c3\u30c8\u7528\u306e\u81ea\u52d5\u5316\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u691c\u7d22\u3084\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u8a73\u7d30\u3092\u898b\u308b<\/a><\/p>\n

 <\/p>\n

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