{"id":6922,"date":"2020-06-05T14:30:29","date_gmt":"2020-06-05T05:30:29","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=6922"},"modified":"2025-03-10T11:05:12","modified_gmt":"2025-03-10T02:05:12","slug":"workshop_spa_bid_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/workshop_spa_bid_ts_1\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011ELISA\u6e2c\u5b9a\u6642\u306b\u3067\u304d\u308b\u30a6\u30a7\u30eb\u5185\u306e\u6c17\u6ce1\u3092\u3068\u308c\u308b\u304b\u691c\u8a3c\u3057\u3066\u307f\u305f"},"content":{"rendered":"<p>ELISA\uff08\u9175\u7d20\u7d50\u5408\u514d\u75ab\u5438\u7740\u30a2\u30c3\u30bb\u30a4\uff09\u306f\u3001\u76ee\u7684\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u691c\u51fa\u304a\u3088\u3073\u5b9a\u91cf\u5316\u3092\u9ad8\u611f\u5ea6\u3067\u884c\u3046\u624b\u6cd5\u306e\u4e00\u3064\u3067\u3059\u3002\u4e00\u65b9\u3001\u9ad8\u611f\u5ea6\u3067\u3042\u308b\u304c\u6545\u306b\u3061\u3087\u3063\u3068\u3057\u305f\u624b\u6280\u4e0a\u306e\u554f\u984c\u304c\u6e2c\u5b9a\u7d50\u679c\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3057\u3066\u3057\u307e\u3046\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u5b9f\u9a13\u6642\u306b\u306f\u3088\u308a\u9ad8\u7cbe\u5ea6\u3001\u3088\u308a\u518d\u73fe\u6027\u306e\u9ad8\u3044\u5b9f\u9a13\u64cd\u4f5c\u3092\u6c42\u3081\u3089\u308c\u307e\u3059\u3002\u305d\u306e\u4e2d\u306e\u4e00\u3064\u3068\u3057\u3066\u300c\u3067\u304d\u308b\u3060\u3051\u6c17\u6ce1\u304c\u3067\u304d\u306a\u3044\u3088\u3046\u306b\u300d\u3068\u3044\u3046\u3053\u3068\u3082\u3088\u304f\u8a00\u308f\u308c\u307e\u3059\u304c\u3001\u3084\u306f\u308a\u6c17\u6ce1\u304c\u6b8b\u3063\u3066\u3057\u307e\u3046\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u6642\u3001\u6ce8\u5c04\u91dd\u3084\u30d4\u30da\u30c3\u30c8\u30c1\u30c3\u30d7\u3067\u3064\u3064\u3044\u3066\u3082\u5c0f\u3055\u306a\u6c17\u6ce1\u306f\u5272\u308c\u305a\u3001\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u3067\u5438\u3044\u53d6\u308b\u3068\u4eca\u5ea6\u306f\u6db2\u91cf\u304c\u5909\u308f\u3063\u3066\u3057\u307e\u3046\u305f\u3081\u3001\u3069\u3046\u3059\u308b\u306e\u304c\u5f97\u7b56\u304b\u60a9\u307e\u3057\u3044\u9650\u308a\u3067\u3059\u3002<\/p>\n<p>\u5f0a\u793e\u3067\u958b\u50ac\u3057\u3066\u3044\u308bELISA<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/lifescience-training.html?CID=Bid_cbu_sbu_r04_jp_cp1359_pjt5762_bid00000_0so_blg_op_lgn_kt_s00_spa3_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0<\/a>\u306b\u3054\u53c2\u52a0\u3044\u305f\u3060\u3044\u305f\u65b9\u306e\u305d\u3093\u306a\u7387\u76f4\u306a\u7591\u554f\u304b\u3089\u3001ELISA\u6e2c\u5b9a\u6642\u306b\u30a6\u30a7\u30eb\u5185\u306b\u6c17\u6ce1\u304c\u751f\u3058\u3066\u3057\u307e\u3046\u3068\u3069\u306e\u7a0b\u5ea6\u306e\u5f71\u97ff\u304c\u51fa\u308b\u306e\u304b\u3084\u3063\u3066\u307f\u307e\u3057\u305f\uff01 \u307e\u305f\u3001\u7c21\u5358\u306a\u89e3\u6c7a\u7b56\u3082\u8003\u3048\u3066\u307f\u307e\u3057\u305f\uff01<\/p>\n<p>\u7686\u3055\u307e\u3082\u3001\u3069\u3046\u3044\u3046\u7d50\u679c\u306b\u306a\u308b\u306e\u304b\u4e88\u60f3\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u2460\u307b\u3068\u3093\u3069\u5f71\u97ff\u306a\u3044<br \/>\n\u2461\u5438\u5149\u5ea6\u304c\u4e0a\u304c\u308b<br \/>\n\u2462\u5438\u5149\u5ea6\u304c\u4e0b\u304c\u308b<\/p>\n<p>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u306e\u8a18\u4e8b\u3067\u3059\uff01<br \/>\n\u30fbELISA\u3092\u59cb\u3081\u305f\u3070\u304b\u308a\u306e\u7814\u7a76\u8005<\/p>\n<h2>\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n<p>\u6750\u6599\uff1a<br \/>\nP38 MAPK (Total) Human ELISA Kit<\/p>\n<p>\u65b9\u6cd5\uff1a<br \/>\n\u30ad\u30c3\u30c8\u306b\u542b\u307e\u308c\u308b\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3092\u5229\u7528\u3057\u3066\u3001\u30ad\u30c3\u30c8\u4ed8\u5c5e\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u5f93\u3063\u3066\u53cd\u5fdc\u3055\u305b\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/lab-equipment\/microplate-instruments\/microplate-readers.html?CID=Bid_cbu_sbu_r04_jp_cp1359_pjt5762_bid00000_0so_blg_op_lgn_kt_s00_spa3_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Thermo Scientific\u2122 Multiskan\u2122 GO \u5438\u5149\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc<\/a>\u3067450 nm\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\uff08n=6\uff09\u3002\u305d\u306e\u5f8c\u3001\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\uff08FINNPIPETTE\uff09\u3092\u7528\u3044\u306696\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u306e\u5404\u30a6\u30a7\u30eb\u306b1 \u00b5L\u30013 \u00b5L\u30015 \u00b5L\u306e\u6c17\u6ce1\u30921\u500b\u52a0\u3048\u3066\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u305d\u308c\u305e\u308c\u306b\u6c17\u6ce1\u3092\u52a0\u3048\u3066\u6e2c\u5b9a\u3092\u884c\u3044\u3001\u6c17\u6ce1\u304c5\u500b\u306b\u306a\u308b\u307e\u3067\u6e2c\u5b9a\u3092\u7e70\u308a\u8fd4\u3057\u307e\u3057\u305f\u3002<\/p>\n<h2>\u7d50\u679c<\/h2>\n<p>1 \u00b5L\u306e\u7a7a\u6c17\u3092\u5165\u308c\u3066\u4f5c\u308a\u51fa\u3057\u305f\u5c0f\u3055\u306a\u6c17\u6ce1\u306e\u5834\u5408\u3001\u6c17\u6ce1\u304c5\u500b\u306b\u5897\u3048\u3066\u3082\u5438\u5149\u5ea6\u306b\u5f71\u97ff\u306f\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u30023 \u00b5L\u306e\u6c17\u6ce1\u306e\u5834\u5408\u30011\u500b\u3067\u306f\u6709\u610f\u5dee\u304c\u898b\u3089\u308c\u307e\u305b\u3093\u304c\u30012\u500b\u4ee5\u4e0a\u306b\u306a\u308b\u3068\u6c17\u6ce1\u306e\u6570\u304c\u5897\u3048\u308b\u306b\u3064\u308c\u3001\u5438\u5149\u5ea6\u306f\u4e0a\u304c\u3063\u3066\u3044\u304d\u307e\u3057\u305f\u30025 \u00b5L\u306e\u6c17\u6ce1\u306e\u5834\u5408\u306f\u6c17\u6ce11\u500b\u304b\u3089\u6709\u610f\u306b\u5438\u5149\u5ea6\u306e\u4e0a\u6607\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6931\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/06\/picture-1.jpg\" alt=\"\" width=\"1082\" height=\"934\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/06\/picture-1.jpg 1082w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/06\/picture-1-300x259.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/06\/picture-1-1024x884.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/06\/picture-1-768x663.jpg 768w\" sizes=\"auto, (max-width: 1082px) 100vw, 1082px\" \/><\/p>\n<h2>\u8003\u5bdf<\/h2>\n<p>\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\u306f\u30a6\u30a7\u30eb\u306e\u4e2d\u5fc3\u90e8\u306b\u5149\u30d3\u30fc\u30e0\u3092\u7167\u5c04\u3057\u3001\u900f\u904e\u3057\u305f\u5149\u91cf\u304b\u3089\u5438\u5149\u5ea6\u3092\u7b97\u51fa\u3057\u307e\u3059\u30021 \u00b5L\u7a0b\u5ea6\u306e\u5c0f\u3055\u3044\u6c17\u6ce1\u306e\u5834\u5408\u3001\u5149\u306e\u901a\u308b\u4e2d\u5fc3\u90e8\u306b\u306f\u5f71\u97ff\u304c\u51fa\u307e\u305b\u3093\u304c\u3001\u6c17\u6ce1\u304c\u5927\u304d\u304f\u306a\u308b\u3001\u3042\u308b\u3044\u306f\u6c17\u6ce1\u304c\u591a\u304f\u306a\u3063\u305f\u6642\u306b\u3001\u305d\u306e\u8868\u9762\u5f35\u529b\u306e\u5f71\u97ff\uff08\u30e1\u30cb\u30b9\u30ab\u30b9\u52b9\u679c\uff09\u306b\u3088\u308a\u3001\u5149\u6563\u4e71\u304c\u751f\u3058\u3066\u672c\u6765\u306e\u5438\u5149\u5ea6\u3088\u308a\u3082\u9ad8\u304f\u306a\u3063\u305f\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<br \/>\n\u306a\u304a\u3001\u4eca\u56de\u4f7f\u7528\u3057\u305fMultiskan\u2122 GO\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\uff08\u5f8c\u7d99\u6a5f\uff1a<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/lab-equipment\/microplate-instruments\/microplate-readers\/multiskan-sky-microplate-spectrophotometer.html?CID=Bid_cbu_sbu_r04_jp_cp1359_pjt5762_bid00000_0so_blg_op_lgn_kt_s00_spa3_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Thermo Scientific\u2122 Multiskan\u2122 Sky<\/a>\uff09\u306f\u76f4\u5f841.2 mm\u306e\u5149\u30d3\u30fc\u30e0\u3092\u7528\u3044\u3066\u5438\u5149\u6e2c\u5b9a\u3092\u884c\u3044\u307e\u3059\u304c\u3001\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\u306b\u3088\u3063\u3066\u30d3\u30fc\u30e0\u5f84\u306f\u7570\u306a\u308b\u305f\u3081\u3001\u6c17\u6ce1\u306e\u5f71\u97ff\u5ea6\u3082\u7570\u306a\u308b\u3053\u3068\u304c\u4e88\u60f3\u3055\u308c\u307e\u3059\u3002<\/p>\n<h2>\u6c17\u6ce1\u306e\u3068\u308a\u65b9<\/h2>\n<p>\u3055\u3066\u3001\u6c17\u6ce1\u304c\u3042\u308b\u3068\u7d50\u679c\u306b\u5f71\u97ff\u3059\u308b\u5834\u5408\u304c\u3042\u308b\u3053\u3068\u306f\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\u304c\u3001\u3053\u306e\u8a18\u4e8b\u306e\u30bf\u30a4\u30c8\u30eb\u306b\u3082\u3042\u308b\u6c17\u6ce1\u306e\u3068\u308a\u65b9\u306b\u3064\u3044\u3066\u300c\u30d8\u30a2\u30c9\u30e9\u30a4\u30e4\u30fc\u3092\u7528\u3044\u308b\u3068\u3088\u3044\u300d\u3068\u3044\u3046\u5642\u3092\u805e\u304d\u3064\u3051\u3001\u3084\u3063\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<p>\u5229\u7528\u3057\u305f\u3082\u306e\uff1a<br \/>\n\u5bb6\u5ead\u7528\u306e\u30d8\u30a2\u30c9\u30e9\u30a4\u30e4\u30fc\uff08\u51b7\u98a8\u30e2\u30fc\u30c9\uff09<\/p>\n<p>\u65b9\u6cd5\u3068\u7d50\u679c\uff1a<br \/>\n\u30d8\u30a2\u30c9\u30e9\u30a4\u30e4\u30fc\u3067\u51b7\u98a8\u3092\u51fa\u3057\u306a\u304c\u3089\u3001ELISA\u30d7\u30ec\u30fc\u30c8\u306b\u4e0a\u65b9\u304b\u3089\u3086\u3063\u304f\u308a\u3068\u8fd1\u3065\u3051\u3066\u3044\u304f\u3068\u3001\u30d7\u30ec\u30fc\u30c8\u306b20\uff5e30 cm\u7a0b\u5ea6\u307e\u3067\u8fd1\u3065\u3044\u305f\u6642\u70b9\u3067\u3042\u3063\u3051\u306a\u304f\u6c17\u6ce1\u304c\u6d88\u3048\u307e\u3057\u305f\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30fb\u6c17\u6ce1\u304c\u591a\u304f\u306a\u308b\u3068\u3001\u3042\u308b\u3044\u306f\u5927\u304d\u304f\u306a\u308b\u3068\u5438\u5149\u5ea6\u306f\u4e0a\u304c\u308a\u307e\u3057\u305f\u3002<br 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href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/lifescience-training.html?CID=Bid_cbu_sbu_r04_jp_cp1359_pjt5762_bid00000_0so_blg_op_lgn_kt_s00_spa3_Social_LAB\" target=\"_blank\" rel=\"noopener 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