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\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u3059\u308b\u3082\u3046\u4e00\u3064\u306e\u5229\u70b9\u306f\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u30a8\u30e9\u30fc\u3092\u6700\u5c0f\u5316\u3067\u304d\u308b\u3053\u3068\u3067\u3059\u3002<\/strong>\u540c\u4e00\u30a6\u30a7\u30eb\u304b\u3089\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u30c7\u30fc\u30bf\u306f\u3001\u540c\u4e00\u306e\u8a66\u6599\u6dfb\u52a0\u64cd\u4f5c\u306b\u7531\u6765\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u30a8\u30e9\u30fc\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u304a\u3088\u3073\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u7d50\u679c\u306b\u540c\u7b49\u306b\u5f71\u97ff\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3053\u306e\u5229\u70b9\u3092\u5229\u7528\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u53cd\u5fa9\u7cbe\u5ea6\u3092\u8a08\u7b97\u3059\u308b\u524d\u306b\u30bf\u30fc\u30b2\u30c3\u30c8\u30c7\u30fc\u30bf\u3092\u540c\u4e00\u30a6\u30a7\u30eb\u306e\u6a19\u6e96\u5316\u30c7\u30fc\u30bf\u3067\u6a19\u6e96\u5316\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u30b7\u30f3\u30b0\u30eb\u30d7\u30ec\u30c3\u30af\u30b9\u6cd5\u306b\u3088\u308a\u89e3\u6790\u3055\u308c\u305f\uff08\u30a6\u30a7\u30eb\u30d9\u30fc\u30b9\u306e\u6a19\u6e96\u5316\u306a\u3057\uff09\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30c7\u30fc\u30bf\u3068\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u6cd5\u306b\u3088\u308a\u884c\u308f\u308c\u305f\u89e3\u6790\u3068\u306e\u6bd4\u8f03\u306b\u3088\u308a\u3001\u30b7\u30f3\u30b0\u30eb\u30d7\u30ec\u30c3\u30af\u30b9\u306e\u30a8\u30e9\u30fc\u306b\u3082\u3088\u308a\u307e\u3059\u304c\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u7cbe\u5ea6\u306e\u5229\u70b9\u304c\u6975\u3081\u3066\u5927\u304d\u304f\u306a\u308b\u53ef\u80fd\u6027\u306e\u3042\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u30b7\u30f3\u30b0\u30eb\u30d7\u30ec\u30c3\u30af\u30b9\u306e\u30a8\u30e9\u30fc\u304c\u6700\u5c0f\u3067\u3042\u308b\u8a66\u6599\u306b\u304a\u3044\u3066\u306f\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u7cbe\u5ea6\u306e\u5229\u70b9\u3082\u6700\u5c0f\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u7cbe\u5ea6\u306e\u5229\u70b9\u306f\u3001\u3088\u308a\u9ad8\u3044\u7cbe\u5ea6\u3092\u5fc5\u8981\u3068\u3059\u308b\u5b9a\u91cf\u7684\u5b9f\u9a13\u306b\u304a\u3044\u3066\u306f\u7279\u306b\u91cd\u8981\u3067\u3059\u3002<\/strong>\u4f8b\u3048\u3070\u3001\u30b3\u30d4\u30fc\u6570\u5909\u7570\u306e\u5b9f\u9a13\u306b\u304a\u3044\u3066\u30011\u30b3\u30d4\u30fc\u306e\u907a\u4f1d\u5b50\u304b\u30892\u30b3\u30d4\u30fc\u3092\u8b58\u5225\u3059\u308b\u306e\u306f2\u500d\u306e\u5909\u5316\u3067\u3042\u308a\u3001\u826f\u597d\u306a\u7cbe\u5ea6\u304c\u5fc5\u8981\u3067\u3059\u3002\u3057\u304b\u3057\u30012\u30b3\u30d4\u30fc\u304b\u30893\u30b3\u30d4\u30fc\u3092\u8b58\u5225\u3059\u308b\u306e\u306f\u308f\u305a\u304b1.5\u500d\u306e\u5909\u5316\u3067\u3042\u308a\u3001\u3055\u3089\u306b\u512a\u308c\u305f\u7cbe\u5ea6\u304c\u8981\u6c42\u3055\u308c\u307e\u3059\u3002\u30de\u30eb\u30c1\u30d7\u30ec\u30af\u30b7\u30f3\u30b0\u306f\u3001\u3053\u306e\u3088\u3046\u306a\u30bf\u30a4\u30d7\u306e\u5b9f\u9a13\u306b\u5fc5\u8981\u3068\u3055\u308c\u308b\u7cbe\u5ea6\u3092\u5f97\u308b\u305f\u3081\u306b\u63a8\u5968\u3055\u308c\u308b\u4e00\u3064\u306e\u65b9\u6cd5\u3067\u3059\u3002<\/p>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30af\u30b7\u30f3\u30b0\u7528\u88c5\u7f6e<\/h2>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u3067\u306f\u901a\u5e38\u540c\u4e00\u306e\u30a6\u30a7\u30eb\u306b\u8907\u6570\u306e\u8272\u7d20\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u88c5\u7f6e\u306f\u540c\u4e00\u306e\u30a6\u30a7\u30eb\u306b\u5b58\u5728\u3059\u308b\u3053\u308c\u3089\u306e\u7570\u306a\u308b\u8272\u7d20\u3092\u6b63\u78ba\u306b\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u4e00\u65b9\u306e\u8272\u7d20\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u4ed6\u65b9\u306e\u8272\u7d20\u306e\u30b7\u30b0\u30ca\u30eb\u3068\u6bd4\u8f03\u3057\u3066\u6975\u3081\u3066\u9ad8\u3044\u5834\u5408\u306b\u304a\u3044\u3066\u3082\u3001\u5404\u8272\u7d20\u306b\u95a2\u3057\u3066\u7279\u7570\u7684\u306b\u6e2c\u5b9a\u3067\u304d\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30af\u30b7\u30f3\u30b0\u306b\u63a8\u5968\u3055\u308c\u308b\u30b1\u30df\u30b9\u30c8\u30ea<\/h2>\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30de\u30eb\u30c1\u30d7\u30ec\u30af\u30b7\u30f3\u30b0\u306b\u6700\u9069\u306a\u86cd\u5149\u30b1\u30df\u30b9\u30c8\u30ea\u306f\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u306b\u304a\u3044\u3066\u5404\u907a\u4f1d\u5b50\u914d\u5217\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306b\u3001\u305d\u308c\u305e\u308c\u7570\u306a\u3063\u305f\u8272\u7d20\u3092\u4f7f\u7528\u3067\u304d\u308b\u3082\u306e\u3067\u3059\u3002\u307b\u3068\u3093\u3069\u306e\u30de\u30eb\u30c1\u30d7\u30ec\u30af\u30b7\u30f3\u30b0\u306f\u3001TaqMan\u30d7\u30ed\u30fc\u30d6\u30d9\u30fc\u30b9\u306e\u30a2\u30c3\u30bb\u30a4\u306b\u4ee3\u8868\u3055\u308c\u308b\u3001\u591a\u91cd\u8272\u7d20\u3001\u9ad8\u7279\u7570\u6027\u30b1\u30df\u30b9\u30c8\u30ea\u3092\u7528\u3044\u3066\u884c\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

RNA\u3092\u542b\u3080\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u306b\u304a\u3044\u3066\u306f\u3001\u4e00\u822c\u7684\u306b1-Step RT-PCR\u3088\u308a\u30822-Step RT-PCR\u304c\u63a8\u5968\u3055\u308c\u307e\u3059\u3002<\/strong>1-StepRT-PCR\u3067\u306f\u3001\u9006\u8ee2\u5199\u3068PCR\u306b\u540c\u6fc3\u5ea6\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u5fc5\u8981\u3068\u306a\u308a\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30af\u30b7\u30f3\u30b0\u306b\u304a\u3051\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u306e\u6700\u9069\u5316\u304c\u5236\u9650\u3055\u308c\u307e\u3059\u30022-Step RT-PCR\u306b\u304a\u3044\u3066\u306f\u3001\u9006\u8ee2\u5199\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u3053\u3068\u306a\u304f\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30af\u30b7\u30f3\u30b0\u7528\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u306e\u6700\u9069\u5316\u3092\u691c\u8a0e\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u7528\u8272\u7d20\u306e\u9078\u629e<\/h2>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u884c\u3046\u5834\u5408\u3001\u691c\u51fa\u3059\u308b\u5404\u907a\u4f1d\u5b50\u306b\u306f\u3001\u305d\u308c\u305e\u308c\u7570\u306a\u308b\u30ec\u30dd\u30fc\u30bf\u30fc\u8272\u7d20\u3092\u7528\u3044\u308b\u4e8b\u304c\u5fc5\u8981\u3067\u3059\u3002<\/strong>\u9078\u629e\u3057\u305f\u30ec\u30dd\u30fc\u30bf\u30fc\u8272\u7d20\u3092\u540c\u4e00\u306e\u30a6\u30a7\u30eb\u5185\u306b\u304a\u3044\u3066\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u88c5\u7f6e\u306b\u3088\u3063\u3066\u52b1\u8d77\u3057\u6b63\u78ba\u306b\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u30e1\u30fc\u30ab\u30fc\u306f\u5404\u88c5\u7f6e\u306b\u3064\u3044\u3066\u63a8\u5968\u3059\u308b\u8272\u7d20\u3092\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002Applied Biosystems\u2122 \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306b\u306f\u8d64\u8272\u30d1\u30c3\u30b7\u30d6\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\uff08ROX\u8272\u7d20\uff09\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u9752\u8272\u306e\u307f\u3092\u52b1\u8d77\u3059\u308b\u88c5\u7f6e\u306f\u3001\u3053\u306eROX\u8272\u7d20\u3092\u30d1\u30c3\u30b7\u30d6\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u3068\u3057\u3066\u52b1\u8d77\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u304c\u3001\u9752\u8272\u52b1\u8d77\u306f\u4e00\u822c\u7684\u306b\u8d64\u8272\u8272\u7d20\u3092\u30ec\u30dd\u30fc\u30bf\u30fc\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\u306b\u306f\u5341\u5206\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

\u30ec\u30dd\u30fc\u30bf\u30fc\u8272\u7d20\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u3084\u907a\u4f1d\u5b50\u7523\u7269\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u9078\u629e\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u9069\u5207\u306a\u8272\u7d20\u306e\u9078\u629e\u3092\u884c\u3046\u3053\u3068\u306b\u3088\u308a\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u3092\u7c21\u7d20\u5316\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/strong>\u4f8b\u3048\u3070\u3001Invitrogen\u2122 FAM\u2122 \u8272\u7d20\u306f\u3001TaqMan\u30d7\u30ed\u30fc\u30d6\u3067\u4f7f\u7528\u3055\u308c\u308b\u6700\u3082\u4e00\u822c\u7684\u306a\u30ec\u30dd\u30fc\u30bf\u30fc\u8272\u7d20\u3067\u3059\u3002\u5f53\u793e\u3067\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30a2\u30c3\u30bb\u30a4\u7528\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u3068\u3057\u3066FAM\u8272\u7d20\u3092\u9078\u629e\u3057\u3001\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u7528\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u3068\u3057\u3066Invitrogen\u2122 VIC\u2122 \u8272\u7d20\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u3053\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u308a\u3001FAM\u8272\u7d20\u6a19\u8b58\u3092\u3057\u305f\u8907\u6570\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3001\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u7528\u306e\u3001VIC \u8272\u7d20\u306e2\u7a2e\u985e\u306e\u8272\u7d20\u3092\u7d44\u307f\u5408\u308f\u305b\u305f\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u3092\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u30c8\u30ea\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3046\u5834\u5408\u306b\u306f\u30013\u756a\u76ee\u306e\u8272\u7d20\u3068\u3057\u3066Invitrogen\u2122 NED\u2122 \u8272\u7d20\u3092FAM\u8272\u7d20\u304a\u3088\u3073VIC \u8272\u7d20\u3068\u3068\u3082\u306b\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002NED\u8272\u7d20\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u540c\u4e00\u306e\u30a6\u30a7\u30eb\u306bTAMRA\u8272\u7d20\u304c\u5b58\u5728\u3057\u306a\u3044\u3088\u3046\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9PCR\u306b\u304a\u3051\u308b\u30d0\u30a4\u30a2\u30b9<\/h2>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9PCR\u3067\u306e\u504f\u308a\uff08\u30d0\u30a4\u30a2\u30b9\uff09\u306f\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u306b\u304a\u3044\u3066\u8d77\u3053\u308a\u5f97\u308b\u597d\u307e\u3057\u304f\u306a\u3044\u73fe\u8c61\u3067\u3001\u30d0\u30a4\u30a2\u30b9\u304c\u304b\u304b\u308b\u3068\u3088\u308a\u591a\u304f\u5b58\u5728\u3059\u308b\u907a\u4f1d\u5b50\u304cDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306b\u3088\u3063\u3066\u504f\u3063\u3066\u5897\u5e45\u3055\u308c\u3001\u5b58\u5728\u91cf\u306e\u5c11\u306a\u3044\u907a\u4f1d\u5b50\u306e\u5897\u5e45\u3092\u6291\u5236\u3057\u3066\u3057\u307e\u3044\u307e\u3059\u3002<\/strong>\u30d0\u30a4\u30a2\u30b9\u3078\u306e\u5bfe\u51e6\u6cd5\u306f\u3088\u308a\u591a\u304f\u5b58\u5728\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u3078\u306ePCR\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u3092\u6e1b\u5c11\u3055\u305b\u308b\u3053\u3068\u3067\u3001\u300c\u30d7\u30e9\u30a4\u30de\u30fc\u30ea\u30df\u30c3\u30c8\u300d\u3068\u547c\u3070\u308c\u3066\u3044\u307e\u3059\u3002\u5236\u9650\u3092\u304b\u3051\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u306f\u3001\u5c11\u306a\u304f\u3068\u3082\u6307\u6570\u95a2\u6570\u7684\u306b\u5897\u5e45\u3057\u3001\u4e14\u3064PCR\u7523\u7269\u304c\u84c4\u7a4d\u3057\u5b58\u5728\u91cf\u306e\u5c11\u306a\u3044\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5897\u5e45\u3092\u963b\u5bb3\u3057\u3066\u3057\u307e\u3046\u524d\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u67af\u6e07\u3059\u308b\u7a0b\u5ea6\u306e\u6fc3\u5ea6\u3067\u3042\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u3092\u3059\u308b\u5834\u5408\u306b\u306f\u3001PCR\u53cd\u5fdc\u306b\u304a\u3051\u308b\u5404\u907a\u4f1d\u5b50\u3084\u907a\u4f1d\u5b50\u7523\u7269\u306e\u7d76\u5bfeDNA\u91cf\u307e\u305f\u306fcDNA\u91cf\u306b\u57fa\u3065\u3044\u3066\u3001\u3069\u306e\u907a\u4f1d\u5b50\u304c\u30d0\u30a4\u30a2\u30b9\u3092\u8d77\u3053\u3059\u53ef\u80fd\u6027\u304c\u3042\u308b\u304b\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u30c7\u30e5\u30d7\u30ec\u30c3\u30af\u30b9\u306b\u304a\u3044\u3066\u6700\u3082\u4e00\u822c\u7684\u306a3\u3064\u306e\u30b7\u30ca\u30ea\u30aa\u3092\u4ee5\u4e0b\u306b\u793a\u3057\u307e\u3059\u3002<\/p>\n

\u30c7\u30e5\u30d7\u30ec\u30c3\u30af\u30b9\u30b7\u30ca\u30ea\u30aa1<\/h3>\n

\u3053\u308c\u304c\u6700\u3082\u4e00\u822c\u7684\u306a\u30b7\u30ca\u30ea\u30aa\u3067\u3059\u3002\u3088\u308a\u591a\u304f\u5b58\u5728\u3059\u308b\u907a\u4f1d\u5b50\u307e\u305f\u306f\u907a\u4f1d\u5b50\u7523\u7269\u304c\u3059\u3079\u3066\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3044\u3066\u540c\u4e00\u306e\u5834\u5408<\/strong>\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30ea\u30df\u30c3\u30c8\u306f\u3001\u6700\u3082\u591a\u304f\u5b58\u5728\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u5bfe\u3057\u3066\u306e\u307f\u884c\u3046\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001\u771f\u6838\u751f\u7269\u306e\u7dcfRNA\u306e20%\u306b\u76f8\u5f53\u3059\u308b18S rRNA \u304c\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3067\u3042\u308b\u3068\u3057\u307e\u3059\u300218S rRNA \u7531\u6765\u306ecDNA\u306f\u3059\u3079\u3066\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3044\u3066\u3001\u3069\u306emRNA\u7531\u6765\u306ecDNA\u3088\u308a\u3082\u591a\u304f\u5b58\u5728\u3057\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u300118S rRNA\u30a2\u30c3\u30bb\u30a4\u306e\u307f\u30d7\u30e9\u30a4\u30de\u30fc\u30ea\u30df\u30c3\u30c8\u3092\u884c\u3046\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n

\u30c7\u30e5\u30d7\u30ec\u30c3\u30af\u30b9\u30b7\u30ca\u30ea\u30aa2<\/h3>\n

2\u3064\u306e\u907a\u4f1d\u5b50\u304c\u3059\u3079\u3066\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3044\u3066\u307b\u307c\u540c\u4e00\u91cf\u5b58\u5728\u3059\u308b\u5834\u5408<\/strong>\u3002\u4e00\u822c\u7684\u306b\u3001Threshold\u304c\u540c\u3058\u3067\u3042\u308b\u3068\u4eee\u5b9a\u3057\u305f\u5834\u5408\u30012\u3064\u306e\u907a\u4f1d\u5b50\u9593\u306eCt \u306e\u5dee\u304c3\u4ee5\u4e0a\u3042\u308b\u5834\u5408\u306f\u907a\u4f1d\u5b50\u91cf\u306f\u540c\u7b49\u3068\u3044\u3048\u307e\u305b\u3093\u3002\u30b3\u30d4\u30fc\u6570\u591a\u578b\u306a\u3069\u306e\u30b2\u30ce\u30e0DNA\u89e3\u6790\u306e\u591a\u304f\u306f\u3053\u306e\u30b7\u30ca\u30ea\u30aa\u306b\u3042\u3066\u306f\u307e\u308a\u307e\u3059\u3002\u30b7\u30ca\u30ea\u30aa2\u306b\u304a\u3044\u3066\u306f2\u3064\u306e\u907a\u4f1d\u5b50\u304c\u307b\u307c\u540c\u6642\u306b\u6307\u6570\u95a2\u6570\u7684\u5897\u5e45\u30e9\u30a4\u30f3\u3092\u793a\u3059\u305f\u3081\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30ea\u30df\u30c3\u30c8\u306f\u5fc5\u8981\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

\u30c7\u30e5\u30d7\u30ec\u30c3\u30af\u30b9\u30b7\u30ca\u30ea\u30aa3<\/h3>\n

\u3053\u306e\u30b7\u30ca\u30ea\u30aa\u306b\u304a\u3044\u3066\u306f\u3001\u3044\u305a\u308c\u306e\u907a\u4f1d\u5b50\u304a\u3088\u3073\u907a\u4f1d\u5b50\u7523\u7269\u3082\u6975\u3081\u3066\u591a\u304f\u5b58\u5728\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u3044\u305a\u308c\u306e\u30a2\u30c3\u30bb\u30a4\u3082\u30d7\u30e9\u30a4\u30de\u30fc\u30ea\u30df\u30c3\u30c8\u306e\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u76f8\u4e92\u4f5c\u7528<\/h2>\n

\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u306b\u95a2\u3059\u308b\u3082\u3046\u4e00\u3064\u306e\u6ce8\u610f\u70b9\u306f\u3001\u7570\u306a\u308b\u30a2\u30c3\u30bb\u30a4\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u9593\u306b\u304a\u3053\u308b\u76f8\u4e92\u4f5c\u7528\u3067\u3059\u3002<\/strong>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u4e2d\u306e\u30a2\u30c3\u30bb\u30a4\u6570\u306e\u5897\u52a0\u306b\u3088\u308a\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u306e\u6570\u3082\u5897\u52a0\u3059\u308b\u305f\u3081\u3001\u305d\u308c\u305e\u308c\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u540c\u58eb\u306e\u53cd\u5fdc\u306e\u76f8\u4e92\u4f5c\u7528\u306e\u30ea\u30b9\u30af\u3082\u5897\u5927\u3057\u307e\u3059\u3002\u4f8b\u3048\u3070\u30014\u3064\u306ePCR\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u542b\u3080\u30c7\u30e5\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u306b\u304a\u3044\u3066\u306f\u30016\u3064\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u304c\u751f\u3058\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u30026\u3064\u306ePCR\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u306f\u300115\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u304c\u751f\u3058\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u5404\u30a2\u30c3\u30bb\u30a4\u306f\u3001\u30b7\u30f3\u30b0\u30eb\u30d7\u30ec\u30c3\u30af\u30b9\u306b\u304a\u3044\u3066\u306f\u826f\u597d\u306a\u6027\u80fd\u3092\u793a\u3057\u307e\u3059\u304c\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u306b\u304a\u3044\u3066\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u540c\u58eb\u306e\u76f8\u4e92\u4f5c\u7528\u304c\u7af6\u5408\u7523\u7269\u3092\u751f\u6210\u3057\u3001\u5897\u5e45\u3092\u6291\u5236\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u540c\u58eb\u306e\u76f8\u4e92\u4f5c\u7528\u306f\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u306b\u4f7f\u7528\u3055\u308c\u308b\u30a2\u30c3\u30bb\u30a4\u304c\u76f8\u540c\u914d\u5217\u3092\u542b\u3080\u5834\u5408\u306b\u751f\u3058\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u76f8\u4e92\u4f5c\u7528\u304c\u8d77\u3053\u308b\u5834\u5408\u306f\u3001\u7570\u306a\u308b\u30a2\u30c3\u30bb\u30a4\u3092\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u306b\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u305d\u306e\u5bfe\u51e6\u6cd5\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n

 <\/p>\n

\u3010\u7121\u6599\u516c\u958b\u4e2d\u3011\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n

\u3053\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7406\u8ad6\u3084\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u306e\u8a2d\u8a08\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u77e5\u8b58\u304c\u63b2\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u59cb\u3081\u305f\u3070\u304b\u308a\u306e\u65b9\u3084\u3053\u308c\u304b\u3089\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

\u3010\u7121\u6599\u516c\u958b\u4e2d\u3011\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0<\/h2>\n

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