{"id":70,"date":"2015-09-08T00:00:00","date_gmt":"2015-09-08T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic4\/"},"modified":"2025-04-17T15:18:02","modified_gmt":"2025-04-17T06:18:02","slug":"qpcr-basic4","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic4\/","title":{"rendered":"\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3001\u3069\u3046\u3057\u3066\u308b?\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c4\u56de"},"content":{"rendered":"

\u3044\u3088\u3044\u3088\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u771f\u9ac4\u306b\u8feb\u3063\u3066\u304d\u307e\u3057\u305f\u306d\uff01\u4eca\u56de\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3067\u826f\u3044\u7d50\u679c\u3092\u5f97\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u201c\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u201d\u304c\u30c6\u30fc\u30de\u3067\u3059\u3002<\/p>\n

\u3055\u3066\u307f\u306a\u3055\u3093\u306f\u3001\u3069\u306e\u3088\u3046\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u304b\uff1f<\/p>\n\n\t

\n\t\t \u3053\u308c\u306f\u7d50\u69cb\u3001\u30e9\u30dc\u3084\u4eba\u306b\u3088\u308a\u307e\u3059\u304c\u3001\u8ad6\u6587\u306b\u63b2\u8f09\u3055\u308c\u305f\u914d\u5217\u3092\u5143\u306b\u81ea\u5206\u3067\u8003\u3048\u3066\u3044\u308b\u65b9\u3084\u3001\u5e02\u8ca9\u3084\u30d5\u30ea\u30fc\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u4f7f\u3046\u3068\u3044\u3046\u65b9\u304c\u591a\u3044\u3088\u3046\u3067\u3059\u3002\u3069\u3061\u3089\u306b\u305b\u3088\u3001\u914d\u5217\u3092\u51dd\u8996\u3057\u3066\u982d\u3092\u60a9\u307e\u305b\u3001\u6761\u4ef6\u691c\u8a0e\u306b\u6642\u9593\u3092\u8cbb\u3084\u3055\u306d\u3070\u306a\u308a\u307e\u305b\u3093\u3002\t<\/blockquote>\n\n\t

<\/p>\n\t\n\t\n

\u305d\u3053\u3067\u304a\u3059\u3059\u3081\u3057\u305f\u3044\u306e\u304c\u3001\u5f0a\u793e\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u6a5f\u5668\u3067\u3059\u3002\u88fd\u54c1\u306b\u4ed8\u5c5e\u3059\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2Primer Express<\/a>\u304c\u3001\u3053\u308c\u307e\u3067\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u306b\u982d\u3092\u60a9\u307e\u305b\u3066\u304d\u305f\u7814\u7a76\u8005\u306e\u307f\u306a\u3055\u307e\u304b\u3089\u3001\u652f\u6301\u3092\u5f97\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u3067\u306f\u65e9\u901f\u3001Primer Express\u306e\u4f7f\u3044\u65b9\u3092\u307f\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u5897\u5e45\u3055\u305b\u305f\u3044\u76ee\u7684\u306e\u9818\u57df\u306e\u914d\u5217\u3092\u8aad\u307f\u8fbc\u307f\u3001\u30dc\u30bf\u30f3\u30921\u56de\u62bc\u3057\u307e\u3059\u3002\u3059\u308b\u3068\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u6700\u9069\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u5019\u88dc\u304c\u81ea\u52d5\u3067\u30ea\u30b9\u30c8\u30a2\u30c3\u30d7\u3055\u308c\u307e\u3059\uff08\u56f3\uff09\u3002<\/p>\n

\"qpcr-basic4-fig1\"<\/a><\/p>\n

\u56f3 Primer Express\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u753b\u9762<\/p>\n

 <\/p>\n

\u30ea\u30b9\u30c8\u30a2\u30c3\u30d7\u3055\u308c\u305f\u914d\u5217\u3092\u3001\u30c6\u30ad\u30b9\u30c8\u30d5\u30a1\u30a4\u30eb\u304b\u3089\u8aad\u307f\u8fbc\u3080\u304b\u3001\u30b3\u30d4\u30fc\uff06\u30da\u30fc\u30b9\u30c8\u306b\u3088\u3063\u3066\u8aad\u307f\u8fbc\u307f\u307e\u3059\u3002\u6b21\u306b\u3001Find Primers\/Probes now\u3068\u3044\u3046\u30dc\u30bf\u30f3\u3092\u62bc\u3057\u307e\u3059\u3002<\/p>\n

\u3054\u89a7\u306e\u3088\u3046\u306a\u7d50\u679c\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u8d64\u3044\u30de\u30fc\u30af\u306e\u90e8\u5206\u306f\u3001\u65e2\u77e5\u306eSNP\u90e8\u4f4d\u306a\u3069\u30d7\u30e9\u30a4\u30de\u30fc\u30fb\u30d7\u30ed\u30fc\u30d6\u3092\u8a2d\u5b9a\u3057\u305f\u304f\u306a\u3044\u5834\u5408\u306b\u3064\u304b\u3044\u307e\u3059\u3002\u9ed2\u304f\u53cd\u8ee2\u3057\u3066\u3044\u308b\u9818\u57df\u306f\u3001\u5897\u5e45\u7523\u7269\u3067\u3059\u3002\u3053\u306e\u7d50\u679c\u304b\u3089\u3001\u5897\u5e45\u7523\u7269\u306e\u77ed\u3055\u304c\u5206\u304b\u308a\u307e\u3059\u3002<\/p>\n\n\t

\n\t\t Primer Express\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u7528\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u4f5c\u6210\u3059\u308b\u305f\u3081\u306b\u81f3\u9069\u5316\u3055\u308c\u3066\u3044\u308b\u306e\u304c\u3001\u6700\u5927\u306e\u7279\u5fb4\u3067\u3059\u3002\u5358\u306a\u308b\u4ed8\u5c5e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3067\u306f\u306a\u304f\u3001\u201c\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0\u306e\u4e00\u90e8\u201d\u3068\u8a00\u3048\u307e\u3059\u306d\u3002\u3082\u3061\u308d\u3093\u3001\u901a\u5e38\u306ePCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u3001nested-PCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u3001RT-PCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u306b\u3082\u5229\u7528\u3067\u304d\u308b\u306e\u3067\u3001\u6d3b\u7528\u306e\u5e45\u306f\u3068\u3066\u3082\u5e83\u3044\u3067\u3059\u3002\t<\/blockquote>\n\n\t

<\/p>\n\t\n\t\n

\u89e3\u6790\u306e\u8cea\u30fb\u52b9\u7387\u3092\u3055\u3089\u306b\u5411\u4e0a\u3055\u305b\u308b\u305f\u3081\u3001\u5f0a\u793e\u3067\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u7528\u8a66\u85acTaqMan\u00ae Gene Expression Assays<\/a>\u3082\u958b\u767a\u3057\u3066\u3044\u307e\u3059\u3002\u30d2\u30c8\u3001\u30de\u30a6\u30b9\u3001\u30e9\u30c3\u30c8\u306a\u3069\u4e3b\u306a\u30e2\u30c7\u30eb\u751f\u7269\u3092\u7528\u3044\u305f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306b\u5bfe\u5fdc\u3057\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u3001\u30d7\u30ed\u30fc\u30d6\u30bb\u30c3\u30c8\u304c\u8a2d\u8a08\u3055\u308c\u305f\u8a66\u85ac\u30ad\u30c3\u30c8\u3067\u3059\u3002\u3053\u306e\u30ad\u30c3\u30c8\u3092\u4f7f\u3048\u3070\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u3084PCR\u306e\u6761\u4ef6\u691c\u8a0e\u3092\u7701\u7565\u3057\u3066\u78ba\u5b9f\u306b\u7d50\u679c\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059<\/strong>\u3002<\/p>\n\n\t

\n\t\t \u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3084\u6761\u4ef6\u691c\u8a0e\u306b\u8cbb\u3084\u3057\u3066\u3044\u305f\u6642\u9593\u3068\u52b4\u529b\u3092\u5225\u306e\u76ee\u7684\u306b\u6d3b\u7528\u3059\u308c\u3070\u3001\u65b0\u3057\u3044\u30b9\u30c6\u30fc\u30b8\u306b\u7814\u7a76\u3092\u5e83\u3052\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3067\u3057\u3087\u3046\u3002\t<\/blockquote>\n\n\t

<\/p>\n\t\n\t\n

\u3075\u3046\u3001\u4fbf\u5229\u306a\u4e16\u306e\u4e2d\u306b\u306a\u3063\u305f\u3082\u3093\u3060\u2026\u3002\u6b21\u56de\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u539f\u7406\u3092\u89e3\u8aac\u3057\u307e\u3059\u3002\u304a\u697d\u3057\u307f\u306b\uff01<\/p>\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30cf\u30f3\u30c9\u30d6\u30c3\u30af \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h2>\n

\u3053\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7406\u8ad6\u3084\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u306e\u8a2d\u8a08\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u77e5\u8b58\u304c\u63b2\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u59cb\u3081\u305f\u3070\u304b\u308a\u306e\u65b9\u3084\u3053\u308c\u304b\u3089\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h2>\n

\u7570\u5e38\u306aS\u5b57\u72b6\u306e\u5897\u5e45\u66f2\u7dda\u3001NTC\u3067\u306e\u5897\u5e45\u691c\u51fa\u3001\u5897\u5e45\u304c\u898b\u3089\u308c\u306a\u3044\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u5b9f\u9a13\u4e2d\u306b\u56f0\u3063\u305f\u969b\u306e\u624b\u52a9\u3051\u306b\u306a\u308b\u60c5\u5831\u3092\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

 <\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3092\u30d5\u30ea\u30fc\u306e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u4efb\u305b\u308b\u304b? \u8ad6\u6587\u306e\u914d\u5217\u3092\u306b\u3089\u3093\u3067\u81ea\u5206\u306e\u982d\u3067\u8003\u3048\u308b\u304b? 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