{"id":7055,"date":"2020-06-10T12:02:14","date_gmt":"2020-06-10T03:02:14","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=7055"},"modified":"2024-09-18T17:16:49","modified_gmt":"2024-09-18T08:16:49","slug":"bigdye_ce_gsd_ts_2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/bigdye_ce_gsd_ts_2\/","title":{"rendered":"\u3042\u3063\u3068\u3044\u3046\u9593\u306b\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\uff01\u30eb\u30fc\u30c1\u30f3\u4f5c\u696d\u306e\u6642\u9593\u77ed\u7e2e\u306bBigDye\u2122 Direct Cycle Sequencing Kit"},"content":{"rendered":"<p>\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u3092\u3057\u3066\u3044\u308b\u3068\u3001\u53cd\u5fdc\u3084\u7cbe\u88fd\u306e\u4f5c\u696d\u30b9\u30c6\u30c3\u30d7\u304c\u591a\u304f\u3001\u6bce\u56de\u540c\u3058\u8a66\u85ac\u3092\u4f7f\u3044\u3001\u540c\u3058\u4f5c\u696d\u3001\u3069\u3053\u304b\u7701\u7565\u3067\u304d\u306a\u3044\u306e\u304b\u3068\u8003\u3048\u3066\u3057\u307e\u3046\u3068\u304d\u306f\u3042\u308a\u307e\u305b\u3093\u304b\uff1f\u3067\u3082\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3063\u3066\u4ed6\u306b\u65b9\u6cd5\u306a\u3044\u3088\u306d\uff1f\u3068\u3001\u534a\u3070\u8ae6\u3081\u306e\u5883\u5730\u306b\u3044\u3089\u3063\u3057\u3083\u308b\u65b9\u3082\u591a\u3044\u306e\u3067\u306f\uff1f\u4eca\u56de\u306f\u3001\u305d\u3093\u306a\u7686\u3055\u3093\u306b\u662f\u975e\u8a66\u3057\u3066\u9802\u304d\u305f\u3044\u3001Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing\/sanger-sequencing-kits-reagents\/bigdye-direct-sanger-sequencing-kit.html?CID=gsd_cap_sbu_r04_jp_cp1349_pjt5618_gsd00000_0so_blg_op_lgn_kt_s00_dne5_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">BigDye\u2122 Direct Cycle Sequencing Kit<\/a>\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<br \/>\n\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<br \/>\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306e\u6642\u9593\u77ed\u7e2e\u3092\u3057\u305f\u3044\u65b9<br \/>\n\u30fb\u540c\u3058\u9818\u57df\u3092\u7e70\u308a\u8fd4\u3057\u30b7\u30fc\u30b1\u30f3\u30b9\u3057\u3066\u3044\u308b\u65b9<\/p>\n<h2>PCR\u53cd\u5fdc\u5f8c\u306e\u30c1\u30e5\u30fc\u30d6\u306b\u3001\u8a66\u85ac\u3092\u6dfb\u52a0\u3057\u3066\u3044\u304f\u3060\u3051\uff01PCR\u5f8c\u306e\u7cbe\u88fd\u3082\u4e0d\u8981\uff01<\/h2>\n<p>\u5f93\u6765\u306e\u30b9\u30c6\u30c3\u30d7\u3092\u6539\u3081\u3066\u78ba\u8a8d\u3057\u3066\u307f\u307e\u3059\u3068\u3001<\/p>\n<p><strong>DNA\u62bd\u51fa\u2192\u76ee\u7684\u9818\u57df\u3092PCR\u3067\u5897\u5e45\u2192PCR\u5f8c\u306e\u7cbe\u88fd\u2192\u6fc3\u5ea6\u78ba\u8a8d\u2192\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u2192\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306e\u7cbe\u88fd\u2192\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3067\u6cf3\u52d5<\/strong><\/p>\n<p>\u3068\u3001\u30c1\u30e5\u30fc\u30d6\u304b\u3089\u307e\u305f\u5225\u306e\u30c1\u30e5\u30fc\u30d6\u3078\u3068\u53cd\u5fdc\u6db2\u3092\u79fb\u3057\u3001\u8a66\u85ac\u3092\u6dfb\u52a0\u3057\u3066\u3001\u3068\u3044\u3046\u4f5c\u696d\u304c\u7d9a\u304d\u3001\u307b\u307c\u4e38\u4e00\u65e5\u304b\u3051\u3066\u306e\u4f5c\u696d\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>\u3053\u3053\u3067\u4eca\u56de\u304a\u52e7\u3081\u3057\u305f\u3044BigDye\u2122 Direct Cycle Sequencing Kit\u306e\u4e2d\u8eab\u3092\u898b\u3066\u307f\u307e\u3057\u3087\u3046\u3002<br \/>\n\u30fbPCR\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9<br \/>\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9<br \/>\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc\uff08M13 Forward\/Reverse\uff09<br \/>\n\u306a\u304a\u3001PCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u3054\u81ea\u8eab\u3067\u7528\u610f\u3044\u305f\u3060\u304f\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u304c\u3001\u3053\u306e\u30ad\u30c3\u30c8\u3055\u3048\u308c\u3042\u308c\u3070\u3001PCR\u53cd\u5fdc\uff5e\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u307e\u3067\u8cc4\u3048\u3066\u3057\u307e\u3046\u3001\u3068\u3044\u3046\u308f\u3051\u3067\u3059\u3002<\/p>\n<p>\u305f\u3060\u3057\u3001\u3054\u6e96\u5099\u3044\u305f\u3060\u304fPCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u3001\u4e00\u3064\u6ce8\u610f\u70b9\u304c\u3042\u308a\u307e\u3059\u3002<br \/>\n\u30ad\u30c3\u30c8\u306e\u5185\u5bb9\u3092\u898b\u3066\u3044\u305f\u3060\u304f\u3068\u308f\u304b\u308b\u901a\u308a\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u3059\u3067\u306bM13 Forward\uff0fM13 Reverse \u3068\u6c7a\u307e\u3063\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001PCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u306f\u3001Forward, Reverse\u3068\u3082\u306b5\u2019\u672b\u7aef\u306bM13\u914d\u5217\u3092\u4ed8\u52a0\u3057\u305fM13 tailed \u30d7\u30e9\u30a4\u30de\u30fc\u3092\u6e96\u5099\u3057\u3001PCR\u3067M13\u306e\u914d\u5217\u3092\u542b\u3080\u65ad\u7247\u3092\u5897\u5e45\u3055\u305b\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u3067\u306f\u3001\u65e9\u901fBigDye\u2122 Direct Cycle Sequencing Kit\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3092\u898b\u3066\u3044\u304d\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<p>\u2460PCR\u53cd\u5fdc\uff08Forward,Reverse\u7528\u306b2\u30c1\u30e5\u30fc\u30d6\u6e96\u5099\uff09<br \/>\n\u30fb\u30c1\u30e5\u30fc\u30d6\u306b\u3001DNA\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3001M13 tailed PCR\u30d7\u30e9\u30a4\u30de\u30fc\uff08Forward,Reverse\uff09\u3001\u30ad\u30c3\u30c8\u306b\u542b\u307e\u308c\u308bPCR\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u6dfb\u52a0\u3057\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u3067PCR\u53cd\u5fdc\uff08\uff5e\u7d0490\u5206\uff09<\/p>\n<p>\u2461\u7cbe\u88fd\uff0b\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u53cd\u5fdc<br \/>\n\u30fbPCR\u53cd\u5fdc\u304c\u7d42\u4e86\u3057\u305f2\u30c1\u30e5\u30fc\u30d6\u306b\u3001\u305d\u308c\u305e\u308c\u30ad\u30c3\u30c8\u306b\u542b\u307e\u308c\u308b\u8a66\u85ac\u3068M13\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u6dfb\u52a0<br \/>\nForward\u7528\uff1a\u30b7\u30fc\u30b1\u30f3\u30b9\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\uff0bM13\u30d7\u30e9\u30a4\u30de\u30fc\uff08Forward\uff09<br \/>\nReverse\u7528\uff1a\u30b7\u30fc\u30b1\u30f3\u30b9\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\uff0bM13\u30d7\u30e9\u30a4\u30de\u30fc\uff08Reverse\uff09<\/p>\n<p>\u3053\u306e\u307e\u307e\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u304b\u3051\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u958b\u59cb\u3067\u3059\u3002\u65e2\u306b\u304a\u6c17\u3065\u304d\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u304c\u3001PCR\u5f8c\u306e\u6eb6\u6db2\u3092\u79fb\u3057\u66ff\u3048\u308b\u5fc5\u8981\u304c\u306a\u3044\u306e\u3067\u3059\uff01\u305d\u3057\u3066\u3001PCR\u53cd\u5fdc\u5f8c\u306e\u7cbe\u88fd\u3082\u5fc5\u8981\u3042\u308a\u307e\u305b\u3093\u3002\u3053\u308c\u306f\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306b\u3001PCR\u5f8c\u306e\u7cbe\u88fd\u8a66\u85ac\u3068\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u7528\u8a66\u85ac\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u305f\u3081\u3067\u3059\u3002\u8a66\u85ac\u3092\u6dfb\u52a0\u3057\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u7f6e\u304f\u3060\u3051\u3067\u3001PCR\u5f8c\u306e\u7cbe\u88fd\u3068\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u304c\u7d42\u4e86\u3002\u3059\u3067\u306b\u3001\u3053\u3053\u307e\u3067\u3067\u7c21\u4fbf\u3055\u3092\u611f\u3058\u3066\u3057\u307e\u3063\u305f\u65b9\u3082\u591a\u3044\u306e\u3067\u306f\u3068\u601d\u3044\u307e\u3059\u304c\u3001\u6b21\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306e\u7cbe\u88fd\u3082\u7c21\u4fbf\u306a\u8a66\u85ac\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u4f75\u305b\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\u3055\u3089\u306bBigDye X Terminator Purification Kit\u3068\u306e\u7d44\u307f\u5408\u308f\u305b\u3067\u3001\u6700\u5f8c\u307e\u30671\u30c1\u30e5\u30fc\u30d6\u3067\u53cd\u5fdc\u53ef\u80fd<\/h2>\n<p>\u7686\u3055\u3093\u306f\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306e\u7cbe\u88fd\u3092\u3069\u306e\u3088\u3046\u306b\u3055\u308c\u3066\u3044\u307e\u3059\u3067\u3057\u3087\u3046\u304b\uff1f<br \/>\n\u30a8\u30bf\u6c88\u3001\u30ab\u30e9\u30e0\u7cbe\u88fd\u306a\u3069\u3055\u308c\u3066\u3044\u308b\u65b9\u3082\u591a\u3044\u3068\u601d\u3044\u307e\u3059\u304c\u3001\u30a8\u30bf\u6c88\u3067\u306f\u30da\u30ec\u30c3\u30c8\u3082\u4e00\u7dd2\u306b\u6368\u3066\u3066\u3057\u307e\u3063\u305f\uff01\u306a\u3093\u3066\u82e6\u3044\u7d4c\u9a13\u306f\u3042\u308a\u307e\u305b\u3093\u304b\uff1f<br \/>\n\u3053\u3053\u3067\u306f\u3001\u305d\u3093\u306a\u304a\u60a9\u307f\u3092\u4e00\u8e74\u3059\u308b\u3001Applied Biosystems\u2122 BigDye XTerminator\u2122 Purification Kit\u3092\u3054\u7d39\u4ecb\u3057\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<p>BigDye Direct Cycle Sequencing Kit\u3067\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u307e\u3067\u884c\u3063\u305f\u5f8c\u3001\u3053\u306e\u7cbe\u88fd\u30ad\u30c3\u30c8\u3092\u4f7f\u7528\u3059\u308c\u3070\u3001PCR\u53cd\u5fdc\uff5e\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u306b\u639b\u3051\u308b\u307e\u3067\u3001\u306a\u3093\u30681\u30c1\u30e5\u30fc\u30d6\u3067\u5b8c\u7d50\u3057\u3066\u3057\u307e\u3046\u306e\u3067\u3059\uff01<br \/>\nBigDye X Terminaor Purification Kit\u306f\u3001<br \/>\n\u30fbSAM solution\uff08\u30d0\u30c3\u30d5\u30a1\u30fc\uff09<br \/>\n\u30fbBigDye X Terminator\uff08\u30d3\u30fc\u30ba)<br \/>\n\u3067\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3053\u306e2\u6db2\u3092\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306e\u30c1\u30e5\u30fc\u30d6\u306b\u6dfb\u52a0\u3001\u305d\u306e\u307e\u307e\u30df\u30ad\u30b5\u30fc\u3067\u652a\u62cc\u3057\u307e\u3059\u3002\u3053\u306e\u6642\u306b\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306b\u4f7f\u7528\u3055\u308c\u306a\u304b\u3063\u305f\u3001\u4f59\u5270\u306eDye\u304c\u30d3\u30fc\u30ba\u306b\u5438\u7740\u3057\u307e\u3059\u3002\u305d\u306e\u5f8c\u9060\u5fc3\u3067\u3001\u4f59\u5270Dye\u304c\u30d3\u30fc\u30ba\u306b\u5438\u7740\u3057\u305f\u307e\u307e\u30c1\u30e5\u30fc\u30d6\u306e\u5e95\u306b\u6c88\u307f\u3001\u4e0a\u6e05\u306b\u306f\u76ee\u7684\u306e\u65ad\u7247\u306e\u307f\u304c\u5b58\u5728\u3059\u308b\u72b6\u614b\u3001\u3068\u306a\u308b\u308f\u3051\u3067\u3059\u3002<br \/>\n\u5f53\u793e\u88fd\u54c1\uff08SeqStuio, 3500\/3500xl, 3130\/3130xl, 3730\/3730xl\/3730xl-LV\uff09\u3092\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u65b9\u306fBigDye X 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