{"id":7096,"date":"2020-06-11T16:08:19","date_gmt":"2020-06-11T07:08:19","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=7096"},"modified":"2025-02-07T16:44:44","modified_gmt":"2025-02-07T07:44:44","slug":"spheroid_nunc_lpd_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/spheroid_nunc_lpd_ts_1\/","title":{"rendered":"in vitro\u306b\u304a\u3051\u308b3D\u304c\u3093\u7d30\u80de\u30e2\u30c7\u30ea\u30f3\u30b0\u3092\u30b5\u30dd\u30fc\u30c8\uff01"},"content":{"rendered":"

\u304c\u3093\u7d30\u80de\u7814\u7a76\u306b\u304a\u3044\u3066\u901a\u5e38\u884c\u308f\u308c\u3066\u3044\u308b\u5358\u5c64\uff082\u6b21\u5143\uff09\u7d30\u80de\u57f9\u990a\u306f\u5b9f\u969b\u306bin vivo<\/em>\u3067\u7d30\u80de\u304c\u53d7\u3051\u3066\u3044\u308b\u8907\u96d1\u306a\u74b0\u5883\u3092\u6b63\u78ba\u306b\u53cd\u6620\u3057\u3066\u3044\u308b\u3068\u306f\u8a00\u3044\u5207\u308c\u307e\u305b\u3093\u3002\u4f8b\u3048\u3070\u3001\u7d30\u80de\u306e\u5f62\u72b6\u306f\u672c\u6765\u306e\u5f62\u306b\u6bd4\u3079\u3066\u5e73\u5766\u306b\u5f15\u304d\u4f38\u3070\u3055\u308c\u3066\u304a\u308a\u30b9\u30c8\u30ec\u30b9\u304c\u304b\u304b\u3063\u305f\u72b6\u614b\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u7d30\u80de\u5206\u5316\u3084\u7d30\u80de\u9593\u7d50\u5408\u306a\u3069\u3082in vivo<\/em>\u306e\u72b6\u614b\u3068\u6bd4\u3079\u308b\u3068\u4f4e\u4e0b\u3057\u307e\u3059\u3002\u7d30\u80de\u306f\u5e38\u306b\u7d30\u80de\u5916\u30de\u30c8\u30ea\u30c3\u30af\u30b9\uff08ECM\uff09\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u306b\u3088\u3063\u3066\u7d30\u80de\u904a\u8d70\u3084\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3001\u8ee2\u5199\u306e\u5236\u5fa1\u3001\u53d7\u5bb9\u4f53\u306e\u767a\u73fe\u3068\u3044\u3063\u305f\u751f\u7269\u5b66\u7684\u6a5f\u80fd\u3092\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3055\u308c\u3066\u3044\u307e\u3059\u3002
\n\u3053\u308c\u306b\u5bfe\u3057\u3066\u30013\u6b21\u5143\u7d30\u80de\u57f9\u990a\u3067\u306f\u7d30\u80de\u306fin vivo<\/em>\u3068\u540c\u69d8\u306b\u81ea\u7136\u306a\u5f62\u72b6\u304c\u4fdd\u305f\u308c\u3066\u3044\u307e\u3059\u3002\u7d30\u80de\u5206\u5316\u3084\u7d30\u80de\u9593\u7d50\u5408\u3082\u6d3b\u767a\u306b\u884c\u308f\u308c\u3001in vivo<\/em>\u306e\u751f\u7406\u5b66\u7684\u74b0\u5883\u306b\u8fd1\u3044\u72b6\u614b\u3092\u7dad\u6301\u3067\u304d\u307e\u3059\u3002
\n\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u304c\u3093\u7d30\u80de\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u5f62\u6210\u3084ES\u7d30\u80de \/ iPS\u7d30\u80de\u306e\u80da\u69d8\u4f53\u5f62\u6210\u306e\u7814\u7a76\u306b\u6709\u52b9\u306a3\u6b21\u5143\u7d30\u80de\u57f9\u990a\u306b\u9069\u3057\u305f\u4f4e\u5438\u7740\u8868\u9762\u51e6\u7406\u5bb9\u5668\u3001Thermo Scientific\u2122 Nunclon\u2122 Sphera\u2122\u8868\u9762\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n

\u25bc\u3053\u3093\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01
\n\u30fbHepG2\u306a\u3069\u306e\u304c\u3093\u7d30\u80de\u306e\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u5f62\u6210
\n\u30fbES\u7d30\u80de \/ iPS\u7d30\u80de\u306e\u80da\u69d8\u4f53\u5f62\u6210
\n\u30fb\u304c\u3093\u7d30\u80de\u3092\u59cb\u3081\u3068\u3059\u308b\u8db3\u5834\u975e\u4f9d\u5b58\u6027\u30b3\u30ed\u30cb\u30fc\u5f62\u6210
\n\u30fb\u5275\u85ac\u3084\u518d\u751f\u533b\u7642\u306e\u7814\u7a76<\/p>\n

Nunclon Sphera\u8868\u9762\u3068\u306f\uff1f<\/h2>\n

\u7d30\u80de\u819c\u3068\u540c\u3058\u6210\u5206\u306e\u30db\u30b9\u30db\u30ea\u30eb\u30b3\u30ea\u30f3\u57fa\u3092\u6709\u3059\u308bMPC\uff082-Methacryloyloxyrthyl Phosphoryl Choline\uff09\u30dd\u30ea\u30de\u30fc\u3092\u57f9\u990a\u5668\u6750\u8868\u9762\u306b\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u7d30\u80de\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5438\u7740\u3092\u8457\u3057\u304f\u6291\u5236\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u306fMPC\u30dd\u30ea\u30de\u30fc\u304c\u8d85\u89aa\u6c34\u6027\u3067\u3042\u308b\u305f\u3081\u306b\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\uff08\u758e\u6c34\u7d50\u5408\uff09\u304c\u307b\u3068\u3093\u3069\u8d77\u3053\u3089\u306a\u3044\u3053\u3068\u3001\u8868\u9762\u306e\u96fb\u8377\u304c\u307b\u307c\u30bc\u30ed\u306b\u7b49\u3057\u3044\u305f\u3081\u9759\u96fb\u6c17\u7684\u7d50\u5408\u304c\u307b\u3068\u3093\u3069\u8d77\u3053\u3089\u306a\u3044\u3053\u3068\u306b\u8d77\u56e0\u3059\u308b\u3082\u306e\u3067\u3059\u3002
\n\u3053\u306e\u6027\u72b6\u306b\u3088\u3063\u30663\u6b21\u5143\u7d30\u80de\u57f9\u990a\u304c\u5bb9\u6613\u306b\u306a\u308a\u307e\u3059\u3002\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u3084\u80da\u69d8\u4f53\u304c\u5747\u4e00\u3067\u518d\u73fe\u6027\u3088\u304f\u5f62\u6210\u3055\u308c\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u6210\u5206\u3092\u542b\u3080\u7d30\u80de\u306e\u5206\u6ccc\u7269\u3084\u5897\u6b96\u56e0\u5b50\u306a\u3069\u306e\u5668\u6750\u8868\u9762\u3078\u306e\u5438\u7740\u306b\u3088\u308b\u30ed\u30b9\u3092\u5927\u5e45\u306b\u4f4e\u6e1b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

Nunclon Sphera\u8868\u9762\u306e\u30d1\u30d5\u30a9\u30fc\u30de\u30f3\u30b9<\/h2>\n

\u2460\u7d30\u80de\u5916\u30de\u30c8\u30ea\u30c3\u30af\u30b9\uff08ECM\uff09\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u975e\u7279\u7570\u7684\u7d50\u5408
\nThermo Scientific\u2122 Nunclon\u2122 Sphera\u2122 96\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u3068Thermo Scientific\u2122 Nunclon\u2122 Delta 96\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u306b\u3001FITC\u6a19\u8b58\u30a6\u30b7\u30b3\u30e9\u30fc\u30b2\u30f3\u30bf\u30a4\u30d7\u2160\u3068TAMRA\u2122\u6a19\u8b58\u30d5\u30a3\u30d6\u30ed\u30cd\u30af\u30c1\u30f3\u3092\u5206\u6ce8\u3057\u3066\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u5f8c\u3001\u86cd\u5149\u5f37\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001Nunclon Sphera\u8868\u9762\u3078\u306eECM\u306e\u7d50\u5408\u306f\u6975\u3081\u3066\u4f4e\u3044\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306fNunclon Sphera\u8868\u9762\u304cECM\u7d50\u5408\u80fd\u3092\u6700\u5c0f\u5316\u3057\u3001\u8868\u9762\u3078\u306e\u5438\u7740\u3092\u963b\u5bb3\u3059\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u2461\u7d30\u80de\u63a5\u7740
\nU937\u7d30\u80de\u3001Vero\u7d30\u80de\u3001A549\u7d30\u80de\u30926\u30a6\u30a7\u30eb\u30de\u30eb\u30c1\u30c7\u30a3\u30c3\u30b7\u30e5\u3067\u57f9\u990a\u3057\u3066\u304b\u3089\u30de\u30eb\u30c1\u30c7\u30a3\u30c3\u30b7\u30e5\u3092\u6d17\u6d44\u3001\u6b8b\u3063\u305f\u7d30\u80de\u3092DPBS\u3067\u6eb6\u89e3\u3057\u3066\u86cd\u5149\u5f37\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001Nunclon Sphera\u8868\u9762\u3078\u306e\u7d30\u80de\u306e\u63a5\u7740\u306f\u3001\u4ed6\u793e\u30d6\u30e9\u30f3\u30c9\u306e\u4f4e\u5438\u7740\u51e6\u7406\u306e\u30de\u30eb\u30c1\u30c7\u30a3\u30c3\u30b7\u30e5\u3084\u672a\u51e6\u7406\u306e\u30de\u30eb\u30c1\u30c7\u30a3\u30c3\u30b7\u30e5\u306b\u6bd4\u3079\u3066\u6975\u3081\u3066\u4f4e\u3044\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u8840\u6e05\u3068\u8c4a\u5bcc\u306aECM\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5438\u7740\u3092\u6291\u5236\u3057\u3066\u3044\u308b\u3053\u3068\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u2462\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u5f62\u6210
\n3\u7a2e\u985e\u306e\u304c\u3093\u7d30\u80de\u682a\uff08HeLa\u3001HepG2\u3001MCF7\uff09\u3092\u64ad\u7a2e\u3057\u30667\u65e5\u9593\u57f9\u990a\u3057\u307e\u3057\u305f\u3002
\n\u305d\u306e\u7d50\u679c\u3001HeLa\u3001HepG2\u3001MCF7\u7d30\u80de\u306fNunclon Sphera\u8868\u9762\u306b\u304a\u3044\u3066\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u5f62\u6210\u6570\u306b\u95a2\u3057\u3066\u826f\u597d\u306a\u7d50\u679c\u3092\u793a\u3057\u307e\u3057\u305f\u3002
\nHeLa\u3000\u7dcf\u7d30\u80de\u6570\uff1a4.0\uff58105<\/sup> cells\/well\u306b\u5bfe\u3057\u3066\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u6570\uff1a70 spheroids\/well
\nHepG2\u3000\u7dcf\u7d30\u80de\u6570\uff1a4.5\uff58105<\/sup> cells\/well\u306b\u5bfe\u3057\u3066\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u6570\uff1a70 spheroids\/well
\nMCF7\u3000\u7dcf\u7d30\u80de\u6570\uff1a3.0\uff58105<\/sup> cells\/well\u306b\u5bfe\u3057\u3066\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u6570\uff1a90 spheroids\/well<\/p>\n

\u304c\u3093\u7d30\u80de\u7814\u7a76\u3078\u306e\u5fdc\u7528 \u2013 \u85ac\u5264\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0<\/h2>\n

\u304c\u3093\u7d30\u80de\u3092\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u5f62\u6210\uff083\u6b21\u5143\u7d30\u80de\u57f9\u990a\uff09\u3055\u305b\u3066\u540c\u3058\u304c\u3093\u7d30\u80de\u306e\u5358\u5c64\u7d30\u80de\u57f9\u990a\uff082\u6b21\u5143\u7d30\u80de\u57f9\u990a\uff09\u3068\u4e00\u7dd2\u306b\u85ac\u5264\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u3092\u6bd4\u8f03\u3057\u305f\u5834\u5408\u3001\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u5f62\u6210\u3055\u305b\u305f\u304c\u3093\u7d30\u80de\u306e\u65b9\u304c\u9ad8\u3044\u62b5\u6297\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u3053\u308c\u306f\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u306e\u8868\u5c64\u306b\u3042\u308b\u7d30\u80de\u304c\u5185\u5074\u306b\u5b58\u5728\u3059\u308b\u7d30\u80de\u3092\u85ac\u5264\u306e\u8cab\u901a\u304b\u3089\u9632\u304e\u3001\u518d\u3073\u7d30\u80de\u5468\u671f\u306b\u5165\u3063\u305f\u6642\u306b\u6b8b\u5b58\u3057\u305f\u5185\u5074\u306e\u7d30\u80de\u304c\u816b\u760d\u3092\u518d\u73fe\u3055\u305b\u3066\u3057\u307e\u3046\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3067\u3059\u3002\u307e\u305f\u3001\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u306f\u907a\u4f1d\u5b50\uff0f\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3084\u60c5\u5831\u4f1d\u9054\u304cin vivo<\/em>\u306b\u985e\u4f3c\u3057\u3066\u3044\u308b\u305f\u3081\u306b\u85ac\u5264\u8010\u6027\u907a\u4f1d\u5b50\u3084\u30de\u30a4\u30af\u30edRNA\u306e\u767a\u73fe\u3092\u4fc3\u3059\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002
\n\u85ac\u5264\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306b\u304c\u3093\u7d30\u80de\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u306f\u3001\u5358\u5c64\u57f9\u990a\u7d30\u80de\u3067\u306f\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u5f15\u304d\u8d77\u3053\u3059\u85ac\u5264\u30ec\u30d9\u30eb\u304c\u5b9f\u969b\u306b\u52b9\u679c\u304c\u3042\u308b\u306e\u304b\u3092\u78ba\u8a8d\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001in vivo<\/em>\u3067\u52b9\u304d\u76ee\u304c\u3042\u308b\u85ac\u5264\u306e\u7279\u5b9a\u3001\u6295\u4e0e\u91cf\u306e\u6c7a\u5b9a\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u307e\u3068\u3081<\/h2>\n

Nunclon Sphera\u8868\u9762\u306e\u7279\u9577<\/strong>
\n\u30fb\u7d30\u80de\u819c\u3068\u540c\u3058\u6210\u5206\u306e\u751f\u4f53\u9069\u5408\u6027\u304c\u3042\u308b\u30db\u30b9\u30db\u30ea\u30eb\u30b3\u30ea\u30f3\u57fa\u3092\u6709\u3057\u3001\u7d30\u80de\u63a5\u7740\u3092\u5236\u5fa1
\n\u30fb\u5f62\u6210\u3057\u305f\u80da\u69d8\u4f53\uff08EB\uff09\u306e\u54c1\u8cea\u306f\u5747\u4e00\u304b\u3064\u5b89\u5b9a\u3067\u9ad8\u3044\u518d\u73fe\u6027\u3092\u5b9f\u73fe
\n\u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u30da\u30d7\u30c1\u30c9\u306b\u5bfe\u3057\u3066\u3082\u9ad8\u3044\u5438\u7740\u6291\u5236\u52b9\u679c
\n\u30fb\u52d5\u7269\u7531\u6765\u6210\u5206\u30d5\u30ea\u30fc<\/p>\n

\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ce\u30fc\u30c8\uff1a\u300cNunclon Sphera\u8868\u9762\u306b\u304a\u3051\u308b\u6d6e\u904a\u72b6\u614b\u3067\u306e\u52b9\u7387\u7684\u306a3\u6b21\u5143\u304c\u3093\u7d30\u80de\u30b9\u30d5\u30a7\u30ed\u30a4\u30c9\u5f62\u6210\u30b5\u30dd\u30fc\u30c8<\/a>\u300d\u3082\u3054\u8acb\u6c42\u304f\u3060\u3055\u3044\u3002
\n3D\u7d30\u80de\u57f9\u990a\u306b\u95a2\u3059\u308b\u60c5\u5831\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n

\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011Gibco\u7d30\u80de\u57f9\u990a\u57fa\u790e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n

\u7d30\u80de\u57f9\u990a\u306b\u95a2\u3059\u308b\u57fa\u790e\u60c5\u5831\u3092\u89e3\u8aac\u3057\u305f\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\uff08\u65e5\u672c\u8a9e\u7248\u3001\u7d04100\u30da\u30fc\u30b8\uff09\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

 <\/p>\n

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