{"id":7320,"date":"2020-06-25T15:50:03","date_gmt":"2020-06-25T06:50:03","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=7320"},"modified":"2024-09-18T17:15:25","modified_gmt":"2024-09-18T08:15:25","slug":"sybr_ce_gsd_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sybr_ce_gsd_ts_1\/","title":{"rendered":"SYBR Green\u6cd5\u3092\u3082\u3061\u3044\u305fPCR\u7523\u7269\u306e\u691c\u8a3c\u3092\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3067\uff01"},"content":{"rendered":"

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\n\u305d\u3053\u3067\u4eca\u56de\u306f\u3001SYBR Green\u6cd5\u3092\u7528\u3044\u305f\u5897\u5e45\u7523\u7269\u306e\u5869\u57fa\u914d\u5217\u3092\u691c\u8a3c\u3059\u308b\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3001\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002
\n\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01
\n\u30fb\u878d\u89e3\u66f2\u7dda\u306e\u307f\u3067SNP\u30bf\u30a4\u30d4\u30f3\u30b0\u3092\u3055\u308c\u3066\u3044\u308b\u65b9
\n\u30fb\u306f\u3058\u3081\u3066SYBR Green\u6cd5\u3092\u7528\u3044\u3089\u308c\u308b\u65b9
\n\u30fb\u9ad8\u89e3\u50cf\u5ea6\u878d\u89e3\u66f2\u7dda\uff08HRM\uff09\u89e3\u6790\u3092\u3055\u308c\u3066\u3044\u308b\u65b9<\/p>\n

\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306b\u5411\u304fSYBR Green\u6cd5\u3092\u3082\u3061\u3044\u305fPCR\u7523\u7269<\/h2>\n

SYBR Green\u6cd5\u306b\u3088\u308b\u5b9f\u9a13\u3067\u306f\u3001\u878d\u89e3\u66f2\u7dda\u306b\u304a\u3044\u3066\u5897\u5e45\u7523\u7269\u306e\u30d4\u30fc\u30af\u304c\u5341\u5206\u306b\u72ec\u7acb\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5897\u5e45\u7523\u7269\u30d4\u30fc\u30af\u304c\u5341\u5206\u306b\u72ec\u7acb\u3057\u3066\u3044\u306a\u3044\u5834\u5408\u306f\u3001\u975e\u7279\u7570\u7684\u7523\u7269\u304c\u5897\u5e45\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002
\n\u975e\u7279\u7570\u7684\u7523\u7269\u306e\u5b58\u5728\u306f\u826f\u597d\u306a\u30b7\u30fc\u30b1\u30f3\u30b9\u7d50\u679c\u304c\u5f97\u3089\u308c\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

SYBR Green\u6cd5\u306b\u3088\u308b\u5897\u5e45\u7523\u7269\u306e\u7cbe\u88fd<\/h2>\n

SYBR Green\u6cd5\u3092\u3082\u3061\u3044\u305fPCR\u53cd\u5fdc\u6db2\u306b\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3084dNTP\u306a\u3069\u4f59\u5270\u6210\u5206\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002
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\n\u4e0b\u8a18\u306e\u3088\u3046\u306b\u72b6\u6cc1\u306b\u5fdc\u3058\u305f\u7cbe\u88fd\u65b9\u6cd5\u3092\u9078\u629e\u3057\u307e\u3059\u3002<\/p>\n

\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u5b58\u5728\u3057\u306a\u3044\u878d\u89e3\u66f2\u7dda\uff1a Applied Biosystems\u2122 ExoSAP-IT\u2122 \u30b7\u30ea\u30fc\u30ba\u30ad\u30c3\u30c8<\/a>
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\u305f\u3060\u3057\u3001\u3053\u306e\u6642\u70b9\u3067\u306f\u307e\u3060SYBR Green\u306f\u6b8b\u5b58\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u5897\u5e45\u7523\u7269\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3068\u7cbe\u88fd<\/h2>\n

SYBR Green\u6cd5\u3092\u7528\u3044\u305fPCR\u306e\u7523\u7269\u306e\u5897\u5e45\u9577\u306f\u6bd4\u8f03\u7684\u77ed\u3044\u305f\u3081\u3001\u5341\u5206\u306a\u5897\u5e45\u91cf\u304c\u5f97\u3089\u308c\u3066\u3044\u308b\u3068\u63a8\u6e2c\u3055\u308c\u307e\u3059\u3002\u4e00\u65b9\u3067SYBR Green\u304c\u6b8b\u5b58\u3057\u3066\u3044\u307e\u3059\u3002
\n\u3053\u308c\u3089\u3092\u3075\u307e\u3048\u4e0b\u8a18\u306e\u6761\u4ef6\u3092\u53c2\u8003\u306b\u3057\u3066\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u5b9f\u65bd\u3057\u307e\u3059\u3002
\n\u30fb\u92f3\u578b\u3068\u306a\u308bSYBR Green\u6cd5\u3092\u3082\u3061\u3044\u305fPCR\u7523\u7269\u306f10 ng\u4ee5\u4e0b\u304c\u9069\u3057\u3066\u3044\u307e\u3059\u3002
\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u8a66\u85ac\u306fApplied Biosystems\u2122 BigDye\u2122 Terminator v1.1 Cycle Sequencing\u30ad\u30c3\u30c8<\/a>\u304c\u5411\u3044\u3066\u3044\u307e\u3059\u3002
\n\u30fb\u8a66\u85ac\u8abf\u88fd\u306f\u6a19\u6e96\u3082\u3057\u304f\u306f2\u500d\u5e0c\u91c8\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u63a8\u5968\u3057\u307e\u3059\u3002
\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u30d7\u30ed\u30b0\u30e9\u30e0\u306f\u3001Fast\u30d7\u30ed\u30b0\u30e9\u30e0\u3082\u5229\u7528\u53ef\u80fd\u3067\u3059\u3002
\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306e\u7cbe\u88fd\u306fApplied Biosystems\u2122 BigDye XTerminator\u2122 \u7cbe\u88fd\u30ad\u30c3\u30c8<\/a>\u3092\u7528\u3044\u307e\u3059\u3002<\/p>\n

\u307e\u3068\u3081<\/h2>\n

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\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u30d7\u30ed\u30b0\u30e9\u30e0\u306f\u3001Fast\u30d7\u30ed\u30b0\u30e9\u30e0\u304c\u5229\u7528\u53ef\u80fd\u3067\u3059\u3002
\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306e\u7cbe\u88fd\u306fApplied Biosystems\u2122 BigDye XTerminator\u2122 \u7cbe\u88fd\u30ad\u30c3\u30c8<\/a>\u3092\u7528\u3044\u307e\u3059\u3002<\/p>\n

\u5b9f\u9a13\u3067\u304a\u56f0\u308a\u306e\u65b9\u306f\u3001\u907a\u4f1d\u5b50\u89e3\u6790\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30c7\u30b6\u30a4\u30f3\u30b5\u30fc\u30d3\u30b9<\/a>\u3092\u691c\u8a0e\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

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\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

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