{"id":744,"date":"2017-03-30T00:00:00","date_gmt":"2017-03-30T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic42\/"},"modified":"2025-04-16T15:22:29","modified_gmt":"2025-04-16T06:22:29","slug":"qpcr-basic42","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic42\/","title":{"rendered":"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u304a\u3051\u308b\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u3068\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30c7\u30b6\u30a4\u30f3\u3067\u6c17\u3092\u3064\u3051\u308b\u3053\u3068"},"content":{"rendered":"<h2>\u30bf\u30fc\u30b2\u30c3\u30c8\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306e\u30b5\u30a4\u30ba\u3001GC\u542b\u91cf\u3001\u4f4d\u7f6e\u304a\u3088\u3073\u7279\u7570\u6027<\/h2>\n<p><strong>\u53cd\u5fdc\u52b9\u7387\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30c7\u30fc\u30bf\u306e\u7cbe\u5ea6\u306b\u3068\u3063\u3066\u6700\u3082\u91cd\u8981\u3067\u3059\u3002<\/strong>\u7406\u8ad6\u4e0a\u306f\u3001PCR\u53cd\u5fdc\u4e2d\u306f\u5404\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u30b5\u30a4\u30af\u30eb\u6bce\u306b\u30b3\u30d4\u30fc\u3055\u308c\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5168\u9577\u5206\u5b50\u304c\u5404\u30b5\u30a4\u30af\u30eb\u30672\u500d\u306b\u5897\u5e45\u3057\u307e\u3059\u3002\u3053\u306e\u72b6\u614b\u304c100%\u306e\u5897\u5e45\u52b9\u7387\u306b\u76f8\u5f53\u3057\u307e\u3059\u3002\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30eb\u304c\u9032\u3080\u306b\u3064\u308c\u3066\u3001\u53cd\u5fdc\u52b9\u7387\u306e\u5909\u52d5\u306f\u5927\u304d\u304f\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001100%\u306e\u5897\u5e45\u52b9\u7387\u304b\u3089\u5916\u308c\u308b\u3053\u3068\u306f\u3001\u8aa4\u3063\u305f\u30c7\u30fc\u30bf\u306b\u3064\u306a\u304c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p><strong>\u53cd\u5fdc\u52b9\u7387\u306e\u5909\u52d5\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u4e00\u3064\u306e\u65b9\u6cd5\u3068\u3057\u3066\u306f\u3001\u6bd4\u8f03\u7684\u77ed\u3044\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5897\u5e45\u3059\u308b\u3053\u3068\u3067\u3059\u3002<\/strong>100\u5869\u57fa\u5bfe\u306e\u9818\u57df\u306e\u5897\u5e45\u306f\u3001\u4f8b\u3048\u30701,200\u5869\u57fa\u5bfe\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5897\u5e45\u3088\u308a\u3082\u30011\u30b5\u30a4\u30af\u30eb\u306b\u304a\u3051\u308b\u5b8c\u5168\u5408\u6210\u306e\u78ba\u7387\u304c\u9ad8\u304f\u306a\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u9577\u3055\u306f\u4e00\u822c\u7684\u306b50\uff5e150\u5869\u57fa\u5bfe\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u77ed\u3044\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u9577\u3055\u306e\u5909\u52d5\u306e\u5f71\u97ff\u3092\u3042\u307e\u308a\u53d7\u3051\u307e\u305b\u3093\u3002\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u304c\u82e5\u5e72\u5206\u89e3\u3057\u3066\u304a\u308a\u3001\u3057\u304b\u3082\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u304c\u9577\u3044\u5834\u5408\u306b\u306f\u3001\u4e0a\u6d41\u304a\u3088\u3073\u4e0b\u6d41\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u540c\u4e00\u306eDNA\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u4e2d\u306b\u76f8\u88dc\u7684\u914d\u5217\u3092\u898b\u3064\u3051\u308b\u78ba\u7387\u304c\u4f4e\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<p><strong>\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306eGC\u542b\u91cf\u304a\u3088\u3073\u4e8c\u6b21\u69cb\u9020\u306f\u3001\u4e0d\u6b63\u78ba\u306a\u30c7\u30fc\u30bf\u306b\u3064\u306a\u304c\u308b\u3082\u3046\u4e00\u3064\u306e\u539f\u56e0\u3068\u306a\u308a\u5f97\u307e\u3059\u3002<\/strong>DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u53cd\u5fdc\u304c\u4e8c\u6b21\u69cb\u9020\u306b\u3088\u308a\u963b\u5bb3\u3055\u308c\u308b\u5834\u5408\u3001\u5404\u30b5\u30a4\u30af\u30eb\u306b\u304a\u3051\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5897\u5e45\u304c\u4e0d\u5b8c\u5168\u306b\u306a\u308a\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002\u7406\u60f3\u7684\u306b\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u6975\u7aef\u306aGC\u3092\u542b\u307e\u306a\u3044\u3001\u4e2d\u7a0b\u5ea6\uff0850%\uff09\u306eGC\u542b\u91cf\u306e\u9818\u57df\u306b\u30a2\u30cb\u30fc\u30eb\u3057\u3066\u5897\u5e45\u3059\u308b\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002cDNA\u3092\u5897\u5e45\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u3092\u8ee2\u5199\u7523\u7269\u306e3\u2032\u672b\u7aef\u4ed8\u8fd1\u306b\u8a2d\u8a08\u3059\u308b\u3053\u3068\u304c\u671b\u307e\u3057\u3044\u3067\u3059\u3002\u3053\u308c\u3089\u3092\u914d\u616e\u3057\u305f\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306f\u3001RNA\u306e\u4e8c\u6b21\u69cb\u9020\u306b\u3088\u308a\u8ee2\u5199\u7523\u7269\u304b\u3089\u306e\u5168\u9577cDNA\u306e\u5408\u6210\u304c\u963b\u5bb3\u3055\u308c\u30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id=\"attachment_1999\" style=\"width: 786px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic42-fig1.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1999\" class=\"size-full wp-image-1999\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic42-fig1.png\" alt=\"qpcr-basic42-fig1\" width=\"776\" height=\"464\" \/><\/a><p id=\"caption-attachment-1999\" class=\"wp-caption-text\">\u56f31 \u9ad8\u5ea6\u306a\u4e8c\u6b21\u69cb\u9020\u3092\u6709\u3059\u308bRNA\u5206\u5b50<\/p><\/div>\n<p><strong>\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u7279\u7570\u6027\u306f\u30c7\u30fc\u30bf\u306e\u7cbe\u5ea6\u306b\u5f71\u97ff\u3059\u308b\u3082\u3046\u4e00\u3064\u306e\u91cd\u8981\u306a\u56e0\u5b50\u3067\u3059\u3002<\/strong>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u7d50\u5408\u90e8\u4f4d\u304c\u30b2\u30ce\u30e0\u4e0a\u306e\u7279\u7570\u7684\u306a\u5834\u6240\u306b\u8a2d\u8a08\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u305d\u3046\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u3088\u308a\u8a66\u6599\u30b2\u30ce\u30e0\u4e2d\u306e\u76f8\u540c\u6027\u306e\u9ad8\u3044\u4ed6\u306e\u90e8\u4f4d\u306e\u914d\u5217\u304c\u5897\u5e45\u3055\u308c\u308b\u53ef\u80fd\u6027\u3092\u6291\u3048\u3089\u308c\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u7528\u306e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u30d7\u30ed\u30b0\u30e9\u30e0\u306f\u3082\u3068\u3068\u306a\u308b\u30b2\u30ce\u30e0\u306b\u5bfe\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u904e\u7a0b\u3092\u81ea\u52d5\u5316\u3057\u3001\u76f8\u540c\u9818\u57df\u3092\u30de\u30b9\u30af\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3053\u308c\u3089\u306e\u4f4d\u7f6e\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u306a\u3044\u3088\u3046\u306b\u3057\u307e\u3059\u3002<\/p>\n<h2>\u30b2\u30ce\u30e0DNA\u3001\u507d\u907a\u4f1d\u5b50\u304a\u3088\u3073\u30a2\u30ec\u30eb\u5909\u7570<\/h2>\n<p><strong>RNA\u8a66\u6599\u4e2d\u3078\u306eDNA\u30ad\u30e3\u30ea\u30fc\u30aa\u30fc\u30d0\u30fc\u306f\u3001\u767a\u73fe\u91cf\u306e\u6e2c\u5b9a\u306b\u304a\u3044\u3066\u554f\u984c\u3068\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/strong>\u30b2\u30ce\u30e0DNA\u304c\u76ee\u7684\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u8ee2\u5199\u7269\u3068\u3068\u3082\u306b\u5897\u5e45\u3055\u308c\u3066\u30c7\u30fc\u30bf\u304c\u7121\u52b9\u3068\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u30b2\u30ce\u30e0DNA\u306e\u30b3\u30f3\u30bf\u30df\u306f\u3001\u9006\u8ee2\u5199\u9175\u7d20\u3092\u542b\u307e\u306a\u3044\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u53cd\u5fdc\u7cfb\uff08RT\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff09\u3092\u8a2d\u5b9a\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u691c\u51fa\u3067\u304d\u307e\u3059\u3002RT\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u7531\u6765\u306eCt\u304c\u3001\u6700\u3082\u5e0c\u91c8\u3055\u308c\u305f\u30bf\u30fc\u30b2\u30c3\u30c8\u304b\u3089\u5f97\u3089\u308c\u308bCt\u3088\u308a\u3082\u9ad8\u3044\u5834\u5408\u306b\u306f\u3001\u30b2\u30ce\u30e0DNA\u306f\u5897\u5e45\u53cd\u5fdc\u306b\u5f71\u97ff\u3057\u3066\u3044\u306a\u3044\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u30b2\u30ce\u30e0DNA\u306fdNTP\u3084\u30d7\u30e9\u30a4\u30de\u30fc\u306a\u3069\u306e\u53cd\u5fdc\u6210\u5206\u3068\u7af6\u5408\u3059\u308b\u305f\u3081\u3001\u53cd\u5fdc\u52b9\u7387\u3092\u4f4e\u4e0b\u3055\u305b\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p><strong>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u304a\u3044\u3066\u30b2\u30ce\u30e0DNA\u30b3\u30f3\u30bf\u30df\u306b\u3088\u308b\u5f71\u97ff\u3092\u907f\u3051\u308b\u305f\u3081\u306e\u6700\u826f\u306e\u65b9\u6cd5\u306f\u3001\u30b2\u30ce\u30e0DNA\u306b\u5b58\u5728\u3057mRNA\u306b\u306f\u5b58\u5728\u3057\u306a\u3044\u30a4\u30f3\u30c8\u30ed\u30f3\u3092\u5229\u7528\u3059\u308b\u3001\u5468\u5230\u306b\u8003\u616e\u3055\u308c\u305f\u30d7\u30e9\u30a4\u30de\u30fc\uff08\u307e\u305f\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u30fb\u30d7\u30ed\u30fc\u30d6\uff09\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3053\u3068\u3067\u3059\u3002<\/strong><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays\/taqman-gene-expression.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Applied Biosystems\u2122 TaqMan\u2122 Gene Expression Assay<\/a>\u306f\u53ef\u80fd\u306a\u9650\u308a\u3001TaqMan\u30d7\u30ed\u30fc\u30d6\u3092\u30a8\u30af\u30bd\u30f3\u2010\u30a8\u30af\u30bd\u30f3\u30b8\u30e3\u30f3\u30af\u30b7\u30e7\u30f3\u4e0a\u306b\u30c7\u30b6\u30a4\u30f3\u3057\u3066\u3044\u307e\u3059\u3002SYBR\u2122 Green \u8272\u7d20\u30d9\u30fc\u30b9\u306e\u691c\u51fa\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u306b\u95a2\u3057\u3066\u306f\u3001\u96a3\u63a5\u3059\u308b\u30a8\u30af\u30bd\u30f3\u4e0a\u306b\u30a2\u30cb\u30fc\u30eb\u3059\u308b\u304b\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u4e00\u3064\u3092\u30a8\u30af\u30bd\u30f3\u2010\u30a8\u30af\u30bd\u30f3\u30b8\u30e3\u30f3\u30af\u30b7\u30e7\u30f3\u306b\u4e0a\u306b\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u4e0a\u6d41\u3068\u4e0b\u6d41\u306ePCR\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u540c\u4e00\u306e\u30a8\u30af\u30bd\u30f3\u5185\u306b\u30a2\u30cb\u30fc\u30eb\u3059\u308b\u3068DNA\u304a\u3088\u3073RNA\u7531\u6765\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u5171\u306b\u5897\u5e45\u3057\u307e\u3059\u3002\u53cd\u5bfe\u306b\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u96a3\u63a5\u3059\u308b\u30a8\u30af\u30bd\u30f3\u4e0a\u306b\u30a2\u30cb\u30fc\u30eb\u3059\u308b\u3068\u3001\u901a\u5e38cDNA\u306e\u307f\u304c\u5897\u5e45\u3055\u308c\u307e\u3059\u3002\u305d\u306e\u7406\u7531\u306f\u30b2\u30ce\u30e0DNA\u7531\u6765\u306e\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u304c\u30a4\u30f3\u30c8\u30ed\u30f3\u914d\u5217\u3092\u542b\u3080\u305f\u3081\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u4f7f\u7528\u3055\u308c\u308b\u6761\u4ef6\u4e0b\u3067\u52b9\u7387\u7684\u306b\u5897\u5e45\u3055\u308c\u308b\u306b\u306f\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u304c\u9577\u304f\u306a\u308a\u3059\u304e\u308b\u305f\u3081\u3067\u3059\u3002<\/p>\n<p><strong>\u507d\u907a\u4f1d\u5b50\u3001\u307e\u305f\u306f\u30b5\u30a4\u30ec\u30f3\u30c8\u907a\u4f1d\u5b50\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u5834\u5408\u306b\u8003\u616e\u3059\u308b\u3079\u304d\u8ee2\u5199\u5909\u7570\u3067\u3059\u3002<\/strong>\u3053\u308c\u3089\u306e\u907a\u4f1d\u5b50\u306f\u3001\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u307e\u305f\u306f\u907a\u4f1d\u5b50\u81ea\u4f53\u306b\u304a\u3051\u308b\u5909\u7570\u307e\u305f\u306f\u518d\u914d\u5217\u306b\u3088\u308a\u975e\u6a5f\u80fd\u7684\u3068\u306a\u3063\u305f\u3001\u73fe\u5b58\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u8a98\u5c0e\u4f53\u3067\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u30d7\u30ed\u30b0\u30e9\u30e0\u306fBLAST\u691c\u7d22\u3092\u5b9f\u884c\u3057\u3001\u507d\u907a\u4f1d\u5b50\u304a\u3088\u3073\u305d\u306emRNA\u7523\u7269\u3092\u907f\u3051\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\u30a2\u30ec\u30eb\u30d0\u30ea\u30a2\u30f3\u30c8\u306f\u540c\u4e00\u306e\u67d3\u8272\u4f53\u5ea7\u306b\u3042\u308b\u30012\u3064\u4ee5\u4e0a\u306e\u7279\u5fb4\u7684\u306a\u907a\u4f1d\u5b50\u578b\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u30d0\u30ea\u30a2\u30f3\u30c8\u304b\u3089\u306e\u8ee2\u5199\u7523\u7269\u9593\u306b\u306f1\u3064\u4ee5\u4e0a\u306e\u5909\u7570\u306e\u9055\u3044\u304c\u3042\u308a\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306b\u304a\u3044\u3066\u30a2\u30ec\u30eb\u30d0\u30ea\u30a2\u30f3\u30c8\u3092\u8003\u616e\u3059\u308b\u5fc5\u8981\u6027\u304c\u3042\u308b\u304b\u3069\u3046\u304b\u306f\u30011\u3064\u4ee5\u4e0a\u306e\u5909\u7570\u304c\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u914d\u5217\u5185\u306b\u5b58\u5728\u3059\u308b\u304b\u3069\u3046\u304b\u306b\u3088\u3063\u3066\u6c7a\u307e\u308a\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u30d0\u30ea\u30a2\u30f3\u30c8\u9593\u306eGC\u542b\u91cf\u306e\u5dee\u304c\u5897\u5e45\u52b9\u7387\u3092\u5909\u52d5\u3055\u305b\u3001\u878d\u89e3\u66f2\u7dda\u306b\u5206\u96e2\u3057\u305f\u30d4\u30fc\u30af\u304c\u751f\u6210\u3059\u308b\u53ef\u80fd\u6027\u3082\u3042\u308a\u3001\u975e\u7279\u7570\u7684\u306a\u5897\u5e45\u3068\u9593\u9055\u3063\u3066\u5224\u65ad\u3055\u308c\u308b\u53ef\u80fd\u6027\u3082\u3042\u308a\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306b\u304a\u3044\u3066\u306f\u3001\u4ea4\u4e92\u306b\u63a5\u5408\u3057\u305f\u30d0\u30ea\u30a2\u30f3\u30c8\u3082\u8003\u616e\u306b\u5165\u308c\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306e\u7279\u7570\u6027\u3001\u30c0\u30a4\u30de\u30fc\u5f62\u6210\u304a\u3088\u3073\u81ea\u5df1\u30d8\u30a2\u30d4\u30f3\u5f62\u6210<\/h2>\n<p><strong>\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306f\u3001\u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u306b\u3088\u308a\u6700\u3082\u983b\u7e41\u306b\u751f\u3058\u307e\u3059\u304c\u3001\u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc\u540c\u58eb\u307e\u305f\u306f\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u540c\u58eb\u306e\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u3001\u307e\u305f\u306f\u5358\u4e00\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u30d8\u30a2\u30d4\u30f3\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3082\u8d77\u3053\u308a\u5f97\u307e\u3059\u3002<\/strong>\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306f\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u3088\u3046\u306b\u3001\u3088\u308a\u8907\u96d1\u306a\u53cd\u5fdc\u306b\u304a\u3044\u3066\u5927\u304d\u306a\u554f\u984c\u3067\u3059\u3002\u30c0\u30a4\u30de\u30fc\u5f62\u6210\u304c\u4e92\u3044\u9055\u3044\u306b\u8d77\u3053\u308b\u3053\u3068\u306f\u5b9f\u969b\u983b\u7e41\u306b\u898b\u3089\u308c\u308b\u73fe\u8c61\u3067\u3059\u304c\u3001\u305d\u306e\u7d50\u679c\u3042\u308b\u7a0b\u5ea6\u306e\u4f38\u9577\u304c\u8d77\u3053\u308a\u3001\u610f\u56f3\u3059\u308b\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306e\u30b5\u30a4\u30ba\u306b\u8fd1\u3065\u304d\u3001\u30b5\u30a4\u30af\u30eb\u304c\u9032\u3080\u306b\u3064\u308c\u3066\u5897\u5e45\u3059\u308b\u7523\u7269\u304c\u751f\u6210\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u3001PCR\u53cd\u5fdc\u306e\u958b\u59cb\u6642\u70b9\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u91cf\u304c\u5c11\u306a\u3044\u307b\u3069\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u751f\u6210\u306e\u53ef\u80fd\u6027\u306f\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u4e00\u822c\u7684\u306b\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u751f\u6210\u306f\u76ee\u7684\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u3088\u308a\u8d77\u3053\u308a\u306b\u304f\u304f\u306a\u308a\u3001\u307e\u305f\u3053\u306e\u73fe\u8c61\u3092\u6700\u5c0f\u9650\u306b\u6291\u5236\u307e\u305f\u306f\u6392\u9664\u3059\u308b\u65b9\u6cd5\u304c\u591a\u6570\u5b58\u5728\u3059\u308b\u3053\u3068\u306f\u3001\u3053\u306e\u554f\u984c\u306e\u30dd\u30b8\u30c6\u30a3\u30d6\u306a\u5074\u9762\u3067\u3059\u3002<\/p>\n<p><strong>\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306b\u95a2\u3059\u308b\u4e3b\u306a\u554f\u984c\u306f\u3001\u507d\u967d\u6027\u7d50\u679c\u3092\u751f\u3058\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3053\u3068\u3067\u3059\u3002<\/strong>\u3053\u306e\u70b9\u306f\u3001SYBR Green I \u8272\u7d20\u306a\u3069\u306eDNA\u7d50\u5408\u8272\u7d20\u3092\u4f7f\u7528\u3059\u308b\u53cd\u5fdc\u306b\u304a\u3044\u3066\u306f\u7279\u306b\u554f\u984c\u3067\u3059\u3002\u3082\u3046\u4e00\u3064\u306e\u554f\u984c\u70b9\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u751f\u6210\u306b\u8d77\u56e0\u3059\u308b\u53cd\u5fdc\u306e\u7af6\u5408\u306b\u3088\u308a\u3001\u53cd\u5fdc\u52b9\u7387\u304c\u597d\u307e\u3057\u3044\u7bc4\u56f2\u3067\u3042\u308b90%\uff5e110%\u306e\u7bc4\u56f2\u3092\u8d85\u3048\u3066\u3057\u307e\u3046\u53ef\u80fd\u6027\u304c\u3042\u308b\u3053\u3068\u3067\u3059\u3002\u6700\u5f8c\u306e\u4e3b\u306a\u554f\u984c\u70b9\u3067\u3042\u308b\u5897\u5e45\u53cd\u5fdc\u306e\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u306e\u72ed\u57df\u5316\u3082\u307e\u305f\u53cd\u5fdc\u52b9\u7387\u306b\u95a2\u9023\u3057\u3066\u304a\u308a\u3001\u53cd\u5fdc\u306e\u611f\u5ea6\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u81ea\u8eab\u304b\u3089\u30b7\u30b0\u30ca\u30eb\u304c\u767a\u751f\u3057\u3066\u3044\u306a\u3044\u5834\u5408\u306b\u304a\u3044\u3066\u3082\uff08TaqMan Assay \u306e\u5834\u5408\u306e\u3088\u3046\u306b\uff09\u3001\u52b9\u7387\u304a\u3088\u3073\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u306f\u5f71\u97ff\u3092\u53d7\u3051\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u69cb\u7bc9\u3057\u305fPCR\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u3092\u89e3\u6790\u3057\u3001\u305d\u308c\u3089\u304c\u30c0\u30a4\u30de\u30fc\u5f62\u6210\u3084\u81ea\u5df1\u30d8\u30a2\u30d4\u30f3\u5f62\u6210\u3092\u8d77\u3053\u3057\u3084\u3059\u3044\u304b\u3069\u3046\u304b\u3092\u6c7a\u5b9a\u3059\u308b\u3044\u304f\u3064\u304b\u306e\u7121\u6599\u306e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u3054\u5229\u7528\u306b\u306a\u308c\u307e\u3059\u3002<\/p>\n<p>AutoDimer \u30d7\u30ed\u30b0\u30e9\u30e0\uff08\u7c73\u56fd\u56fd\u7acb\u6a19\u6e96\u6280\u8853\u7814\u7a76\u6240\u306eP.M. Vallone \u8457\uff09\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u5168\u30ea\u30b9\u30c8\u3092\u540c\u6642\u306b\u89e3\u6790\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306a\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u306e\u30c4\u30fc\u30eb\u3067\u3059\uff08\u56f32\uff09\u3002\u3053\u306e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306f\u3001\u7279\u306b\u30de\u30eb\u30c1\u30d7\u30ec\u30af\u30b7\u30f3\u30b0\u306b\u304a\u3044\u3066\u6709\u7528\u3067\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u306e\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u89e3\u6790\u306f\u3001\u30c0\u30a4\u30de\u30fc\u751f\u6210\u306e\u30ea\u30b9\u30af\u3092\u5927\u304d\u304f\u4f4e\u6e1b\u3055\u305b\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u304c\u3001\u30c0\u30a4\u30de\u30fc\u5f62\u6210\u3092\u5b9f\u9a13\u7684\u306b\u30e2\u30cb\u30bf\u30fc\u3059\u308b\u3053\u3068\u3082\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<div id=\"attachment_2000\" style=\"width: 720px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic42-fig2.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2000\" class=\"size-full wp-image-2000\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic42-fig2.png\" alt=\"qpcr-basic42-fig2\" width=\"710\" height=\"504\" \/><\/a><p id=\"caption-attachment-2000\" class=\"wp-caption-text\">\u56f32 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href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cloning\/vector-nti-software.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Invitrogen\u2122 Vector NTI\u2122 Software<\/a>\u306e\u914d\u5217\u89e3\u6790\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306a\u3069\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u30d7\u30ed\u30b0\u30e9\u30e0\u306f\u3001\u5f0a\u793e\u306e\u30aa\u30f3\u30e9\u30a4\u30f3\u6ce8\u6587\u30b7\u30b9\u30c6\u30e0\u306b\u76f4\u7d50\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u914d\u5217\u3092\u30ab\u30c3\u30c8\u30fb\u30a2\u30f3\u30c9\u30fb\u30da\u30fc\u30b9\u30c8\u3059\u308b\u5fc5\u8981\u3082\u4e00\u5207\u3042\u308a\u307e\u305b\u3093\u3002\u3053\u308c\u3089\u306e\u30d7\u30ed\u30b0\u30e9\u30e0\u306f\u3001\u4ee5\u4e0b\u306e\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u3092\u7d44\u307f\u8fbc\u3093\u3060\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u3092\u4f7f\u7528\u3057\u3066\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u307e\u305f\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u7528\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u81ea\u52d5\u7684\u306b\u30c7\u30b6\u30a4\u30f3\u3057\u3001\u3055\u3089\u306b\u65e2\u77e5\u306e\u914d\u5217\u3001\u76f8\u540c\u914d\u5217\u306b\u95a2\u3059\u308b\u30b2\u30ce\u30e0\u5168\u4f53\u306eBLAST\u691c\u7d22\u3082\u884c\u3046\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<ul>\n<li>\u901a\u5e38\u300118\uff5e28\u306e\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u9577\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3002<\/li>\n<li>\u7e70\u308a\u8fd4\u3057\u5869\u57fa\u914d\u5217\u3092\u907f\u3051\u308b\u3002<\/li>\n<li>\u30df\u30b9\u30de\u30c3\u30c1\u914d\u5217\u304c\u5b89\u5b9a\u3057\u3066\u30cf\u30a4\u30d6\u30ea\u3059\u308b\u306e\u3092\u9632\u3050\u305f\u3081\u306b\u300150%\u306eGC\u542b\u91cf\u3092\u76ee\u6a19\u3068\u3059\u308b\u3002<\/li>\n<li>Tm\u5024\u304c\u8fd1\u3044\uff08\u304a\u4e92\u3044\u306b2\u00b0C\u4ee5\u5185\uff09\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u9078\u629e\u3059\u308b\u3002<\/li>\n<li>\u30a2\u30c3\u30bb\u30a4\u306b\u4f7f\u7528\u3059\u308b\u3059\u3079\u3066\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u9593\u304a\u3088\u3073\u5404\u30d7\u30e9\u30a4\u30de\u30fc\u5185\u306e\u914d\u5217\u7684\u76f8\u88dc\u6027\u3092\u907f\u3051\u308b\u3002<\/li>\n<\/ul>\n<h2>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30cf\u30f3\u30c9\u30d6\u30c3\u30af 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