{"id":783,"date":"2017-04-18T00:00:00","date_gmt":"2017-04-18T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic45\/"},"modified":"2025-04-16T15:13:26","modified_gmt":"2025-04-16T06:13:26","slug":"qpcr-basic45","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic45\/","title":{"rendered":"\u3042\u306a\u305f\u304c\u4f7f\u3063\u3066\u3044\u308b\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306f\u5927\u4e08\u592b\uff1f 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a\u3068\u91cf\u3001\u304a\u3088\u3073RT\u3068\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u4e21\u8005\u306b\u304a\u3051\u308b\u5897\u5e45\u52b9\u7387\u306e\u5dee\u3092\u6a19\u6e96\u5316\u3057\u307e\u3059\u3002\u3082\u30572\u3064\u306e\u7570\u306a\u308b\u8a66\u6599\u306b\u304a\u3044\u3066\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u9006\u8ee2\u5199\u307e\u305f\u306fDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306b\u3088\u308b\u5897\u5e45\u304c\u7570\u306a\u308b\u901f\u5ea6\u3067\u9032\u884c\u3057\u305f\u5834\u5408\u306b\u306f\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u8ee2\u5199\u304c\u3053\u308c\u3089\u306e\u5909\u52d5\u3092\u53cd\u6620\u3057\u307e\u3059\u3002\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u907a\u4f1d\u5b50\u306b\u306f\u201c\u30cf\u30a6\u30b9\u30ad\u30fc\u30d4\u30f3\u30b0\u201d\u907a\u4f1d\u5b50\u306a\u3069\u306e\u5185\u5728\u6027\u306e\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u907a\u4f1d\u5b50\u307e\u305f\u306f\u5916\u5728\u6027\u306e\u6838\u9178\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n<h3>\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb<\/h3>\n<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u304a\u3051\u308b\u4e00\u822c\u7684\u306a\u5185\u5728\u6027\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u907a\u4f1d\u5b50\u306f\u4ee5\u4e0b\u306e\u901a\u308a\u3067\u3059\u3002<\/p>\n<ul>\n<li>\u03b2-\u30a2\u30af\u30c1\u30f3\uff08ACTB\uff09\uff1a\u7d30\u80de\u9aa8\u683c\u907a\u4f1d\u5b50<\/li>\n<li>18S\u30ea\u30dc\u30bd\u30fc\u30e0RNA\uff08rRNA\uff09\uff1a\u30ea\u30dc\u30bd\u30fc\u30e0\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8<\/li>\n<li>\u30b7\u30af\u30ed\u30d5\u30a3\u30ea\u30f3A\uff08CYC\uff09\uff1a\u30bb\u30ea\u30f3\u30fb\u30c8\u30ec\u30aa\u30cb\u30f3\u30d5\u30a9\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u963b\u5bb3\u5264<\/li>\n<li>\u30b0\u30ea\u30bb\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9\u30ea\u30f3\u9178\u30c7\u30d2\u30c9\u30ed\u30b2\u30ca\u30fc\u30bc\uff08GAPDH\uff09\uff1a\u89e3\u7cd6\u7cfb<\/li>\n<li>\u03b2-2-\u30de\u30a4\u30af\u30ed\u30b0\u30ed\u30d6\u30ea\u30f3\uff08B2M\uff09\uff1a\u4e3b\u8981\u7d44\u7e54\u9069\u5408\u6027\u8907\u5408\u4f53<\/li>\n<li>\u03b2-\u30b0\u30eb\u30af\u30ed\u30cb\u30c0\u30fc\u30bc\uff08GUS\uff09\uff1a\u30ea\u30bd\u30bd\u30fc\u30e0\u4e2d\u306e\u30a8\u30ad\u30bd\u30b0\u30ea\u30b3\u30b7\u30c0\u30fc\u30bc<\/li>\n<li>\u30d2\u30dd\u30ad\u30b5\u30f3\u30c1\u30f3\u30ea\u30dc\u30b7\u30eb\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc\uff08HPRT\uff09\uff1a\u30d7\u30ea\u30f3\u518d\u5229\u7528\u7d4c\u8def<\/li>\n<li>TATA-Box\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\uff08TBP\uff09\uff1aRNA\u8ee2\u5199<\/li>\n<\/ul>\n<p><strong>\u3059\u3079\u3066\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u5b9f\u9a13\u306f\u305d\u308c\u305e\u308c\u7570\u306a\u308b\u305f\u3081\u3001\u719f\u616e\u3068\u7dbf\u5bc6\u306a\u8a08\u753b\u306e\u3082\u3068\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059<\/strong>\u3002\u4ed6\u306e\u7814\u7a76\u5ba4\u3067\u4f55\u304c\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u304b\u306b\u57fa\u3065\u3044\u3066\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3092\u9078\u629e\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u9078\u629e\u3057\u305f\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5b9a\u91cf\u6cd5\u306b\u6700\u3082\u3088\u304f\u9069\u5fdc\u3059\u308b\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3092\u9078\u629e\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><strong>\u54c1\u8cea\u304c\u9ad8\u3044\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u7b2c\u4e00\u6761\u4ef6\u306f\u3001\u5b58\u5728\u91cf\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u907a\u4f1d\u5b50\u7523\u7269\u3068\u540c\u7b49\u3067\u3042\u308b\u3053\u3068<\/strong>\u3067\u3059\u3002\u30de\u30eb\u30c1\u30d7\u30ec\u30af\u30b7\u30f3\u30b0\u306b\u304a\u3044\u3066\u306f\u7279\u306b\u91cd\u8981\u3067\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u304cCt\u306b\u5230\u9054\u3059\u308b\u524d\u306b\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u304c\u30d7\u30e9\u30c8\u30fc\u306b\u9054\u3057\u3066\u3057\u307e\u3046\u3068\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306eCt\u5024\u304c\u9ad8\u304f\u306a\u308a\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u610f\u5473\u304c\u306a\u304f\u306a\u308a\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u53cd\u5fdc\u3092\u767a\u73fe\u91cf\u304c\u4f4e\u3044\u53cd\u5fdc\u7cfb\u306b\u3042\u308f\u305b\u308b\u305f\u3081\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u30ea\u30df\u30c3\u30c8\u306e\u6761\u4ef6\u3092\u69cb\u7bc9\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u307b\u3068\u3093\u3069\u306e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays\/taqman-gene-expression\/single-tube-taqman-gene-expression-analysis\/taqman-endogenous-controls.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr51_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Applied Biosystems\u2122 TaqMan\u2122 Endogenous Control Assay<\/a>\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30ea\u30df\u30c3\u30c8\u7528\u306e\u5f62\u614b\u3067\u5165\u624b\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u30bf\u30fc\u30b2\u30c3\u30c8\u7528\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30a2\u30c3\u30bb\u30a4\u306f\u3001\u5b9f\u9a13\u30bf\u30fc\u30b2\u30c3\u30c8\u7528\u306e\u30a2\u30c3\u30bb\u30a4\u3068\u540c\u69d8\u306e\u5897\u5e45\u52b9\u7387\u3092\u6709\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u304c\u3001\u3053\u306e\u70b9\u306f\u691c\u91cf\u7dda\u3092\u4f7f\u7528\u3057\u3066\u8a55\u4fa1\u3067\u304d\u307e\u3059\u3002\u52b9\u7387\u306e\u7570\u306a\u308b\u53cd\u5fdc\u3092\u6bd4\u8f03\u3059\u308b\u5834\u5408\u306b\u306f\u88dc\u6b63\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u304c\u3001\u53cd\u5fdc\u52b9\u7387\u304c\u304a\u4e92\u3044\u306b\u8fd1\u4f3c\u3057\u3066\u3044\u308b\u65b9\u304c\u7cbe\u5ea6\u304c\u5411\u4e0a\u3057\u307e\u3059\u3002<\/p>\n<p><strong>\u6700\u5f8c\u306b\u6700\u3082\u91cd\u8981\u306a\u70b9\u3068\u3057\u3066\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u767a\u73fe\u306f\u8a66\u6599\u306e\u51e6\u7406\u6cd5\u307e\u305f\u306f\u75be\u60a3\u72b6\u614b\u306b\u304b\u304b\u308f\u3089\u305a\u4e00\u5b9a\u3067\u3042\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059<\/strong>\u3002\u3053\u306e\u70b9\u306f\u56f3\u306b\u793a\u3059\u3088\u3046\u306b\u3001\u5b9f\u9a13\u7684\u306b\u6c7a\u5b9a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<div id=\"attachment_2069\" style=\"width: 763px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic45-fig.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2069\" class=\"size-full wp-image-2069\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic45-fig.png\" alt=\"qpcr-basic45-fig\" width=\"753\" height=\"502\" \/><\/a><p id=\"caption-attachment-2069\" class=\"wp-caption-text\">\u56f3 \u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u308b\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u91cf\u3068\u3001\u6a19\u6e96\u5316\u306e\u91cd\u8981\u6027\u3002\u3053\u306e\u4f8b\u3067\u306f\u30012\u3064\u306e\u51e6\u7406\u7fa4\u30681\u3064\u306e\u6b63\u5e38\u7fa4\u306b\u95a2\u3057\u3066\u3001\u5185\u90e8\u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff08IPC\uff09\u3068\u3068\u3082\u306b\u5897\u5e45\u3057\u305f\u4e00\u822c\u7684\u306a\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u3092\u89e3\u6790\u3057\u307e\u3057\u305f\u3002IPC\u306f\u6b63\u5e38\u306a\u53cd\u5fdc\u9593\u306e\u5909\u52d5\u306e\u6a19\u6e96\u5024\u3092\u3057\u3081\u3057\u307e\u3059\u3002\u0394Ct\u304c0\u3067\u3042\u308b\u6b63\u5e38\u8a66\u6599\u3068\u6bd4\u8f03\u3057\u305f\u3001\u5404\u51e6\u7406\u7fa4\u306b\u304a\u3051\u308b\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u306e\u5909\u52d5\u3092\u68d2\u30b0\u30e9\u30d5\u3067\u8868\u3057\u3066\u3044\u307e\u3059\u3002\u76ee\u7684\u306f\u3001IPC\u304c\u793a\u3059\u5909\u5316\u3068\u540c\u69d8\u306e\u5909\u5316\u3092\u793a\u3059\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3092\u898b\u3064\u3051\u308b\u3053\u3068\u3067\u3059\u3002<\/p><\/div>\n<p>\u8907\u6570\u306e\u53cd\u5fa9\u5b9f\u9a13\u3092\u304f\u308a\u304b\u3048\u3059\u3053\u3068\u3067\u6b63\u78ba\u306a\u7d50\u679c\u3067\u3042\u308b\u3053\u3068\u304c\u4fdd\u8a3c\u3055\u308c\u307e\u3059\u304c\u3001\u30b7\u30af\u30ed\u30d5\u30a3\u30ea\u30f3\u3001TBP\u304a\u3088\u3073HPRT\u306f\u3001\u51e6\u7406\u306b\u3088\u308a\u767a\u73fe\u30ec\u30d9\u30eb\u304c\u4e0b\u304c\u3063\u3066\u3044\u308b\u3088\u3046\u306b\u898b\u3048\u308b\u305f\u3081\u3001\u3053\u308c\u3089\u306e\u51e6\u7406\u7fa4\u306b\u95a2\u3057\u3066\u306f\u826f\u597d\u306a\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u9078\u629e\u80a2\u3067\u306f\u306a\u3044\u3053\u3068\u304c\u660e\u3089\u304b\u3067\u3059\u3002<strong>\u6700\u3082\u4e00\u822c\u7684\u306a\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u3067\u3082\u3001\u4e00\u5b9a\u306e\u6761\u4ef6\u4e0b\u3067\u306f\u5909\u5316\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u5e38\u306b\u691c\u8a3c\u3092\u884c\u3046\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059<\/strong>\u3002<\/p>\n<ul>\n<li><strong>GAPDH<\/strong>\u306f\u591a\u304f\u306e\u4f8b\u306b\u304a\u3044\u3066\u4e00\u8cab\u6027\u3092\u793a\u3059\u4e00\u822c\u7684\u306a\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3067\u3059\u3002\u3057\u304b\u3057\u3001<strong>\u3044\u304f\u3064\u304b\u306e\u764c\u7d30\u80de\u3001\u816b\u760d\u6291\u5236\u56e0\u5b50\u3067\u51e6\u7406\u3057\u305f\u7d30\u80de\u3001\u4f4e\u9178\u7d20\u72b6\u614b\u304a\u3088\u3073\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u307e\u305f\u306f\u30a4\u30f3\u30b9\u30ea\u30f3\u51e6\u7406\u3057\u305f\u8a66\u6599\u306b\u304a\u3044\u3066\u306f\u3001\u904e\u5270\u767a\u73fe\u3055\u308c\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059<\/strong>\u3002<\/li>\n<li><strong>\u03b2-\u30a2\u30af\u30c1\u30f3<\/strong>\u306f\u3001\u307b\u3068\u3093\u3069\u306e\u7d30\u80de\u578b\u306b\u304a\u3044\u3066\u6bd4\u8f03\u7684\u5341\u5206\u306a\u767a\u73fe\u91cf\u3092\u793a\u3059\u305f\u3081\u3001\u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u3082\u3046\u4e00\u3064\u306e\u30cf\u30a6\u30b9\u30ad\u30fc\u30d4\u30f3\u30b0\u907a\u4f1d\u5b50\u3067\u3059\u3002\u3057\u304b\u3057\u305d\u306e\u4e00\u8cab\u6027\u306f\u3001<strong>\u4e73\u623f\u4e0a\u76ae\u7d30\u80de\u3001\u5272\u7403\u3001\u30d6\u30bf\u7d44\u7e54\u304a\u3088\u3073\u30a4\u30cc\u5fc3\u7b4b\u306a\u3069\u591a\u304f\u306e\u6761\u4ef6\u4e0b\u3067\u7591\u554f\u8996\u3055\u308c\u3066\u3044\u307e\u3059<\/strong>\u3002<\/li>\n<li><strong>18S rRNA<\/strong>\u306f\u7d30\u80de\u306e\u7dcfRNA\u306e85%\uff5e90%\u3092\u5360\u3081\u3001\u305d\u306e\u91cf\u306f\u30e9\u30c3\u30c8\u809d\u81d3\u3001\u30d2\u30c8\u76ae\u819a\u7dda\u7dad\u82bd\u7d30\u80de\u304a\u3088\u3073\u30d2\u30c8\u3068\u30de\u30a6\u30b9\u306e\u60aa\u6027\u7d30\u80de\u682a\u306b\u304a\u3044\u3066\u975e\u5e38\u306b\u4e00\u8cab\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001<strong>\u4e2d\u7a0b\u5ea6\u307e\u305f\u306f\u4f4e\u3044\u767a\u73fe\u91cf\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u6a19\u6e96\u5316\u306b\u306f\u3001\u305d\u306e\u5b58\u5728\u91cf\u304c\u554f\u984c\u3067\u3059<\/strong>\u300218S rRNA\u3067\u5341\u5206\u306a\u30ec\u30f3\u30b8\u306e\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u304c\u5f97\u3089\u308c\u3001\u304b\u3064\u7814\u7a76\u5bfe\u8c61\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u304c40\u30b5\u30a4\u30af\u30eb\u4ee5\u5185\u306bCt\u306b\u9054\u3059\u308b\u3088\u3046\u306aRNA\u6fc3\u5ea6\u3092\u898b\u3064\u3051\u308b\u3053\u3068\u306f\u591a\u304f\u306e\u5834\u5408\u56f0\u96e3\u3067\u3059\u3002\u3055\u3089\u306b\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u306b\u304a\u3044\u3066\u306f\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u304c\u7814\u7a76\u5bfe\u8c61\u30bf\u30fc\u30b2\u30c3\u30c8\u306ePCR\u52b9\u7387\u3092\u4f4e\u4e0b\u3055\u305b\u306a\u3044\u3088\u3046\u306b\u300118S\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u6fc3\u5ea6\u3092\u5236\u9650\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002<\/li>\n<\/ul>\n<p>\u5358\u4e00\u306e\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u907a\u4f1d\u5b50\u306e\u7cbe\u5ea6\u306b\u4f9d\u5b58\u305b\u305a\u3001\u8907\u6570\u306e\u691c\u8a3c\u6e08\u307f\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u5e7e\u4f55\u5b66\u7684\u5e73\u5747\u5024\u3092\u5229\u7528\u3059\u308b\u4ee3\u66ff\u6cd5\u3082\u5b58\u5728\u3057\u307e\u3059\u3002\u8907\u6570\u306e\u5b89\u5b9a\u3057\u305f\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3092\u4f7f\u7528\u3059\u308b\u3053\u306e\u65b9\u6cd5\u306f\u3001\u3042\u3089\u3086\u308b\u5358\u4e00\u907a\u4f1d\u5b50\u306eCt \u5909\u52d5\u3092\u3088\u308a\u52b9\u679c\u7684\u306b\u548c\u3089\u3052\u3001\u5229\u7528\u3067\u304d\u308b\u30a2\u30c3\u30bb\u30a4\u304a\u3088\u3073\u8a66\u6599\u30bf\u30a4\u30d7\u306e\u7bc4\u56f2\u3092\u5e83\u3052\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h3>\u5916\u6765\u6027\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc<\/h3>\n<p>\u5916\u6765\u6027\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u4f7f\u7528\u306f\u3042\u307e\u308a\u4e00\u822c\u7684\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001<strong>\u7279\u5b9a\u306e\u8a66\u6599\u30bb\u30c3\u30c8\u306b\u5bfe\u3057\u3066\u4e00\u8cab\u6027\u306e\u9ad8\u3044\u5185\u5728\u6027\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u304c\u898b\u3064\u3051\u3089\u308c\u306a\u3044\u5834\u5408\u306b\u4f7f\u7528\u53ef\u80fd\u306a\u4ee3\u66ff\u6cd5\u3067\u3059<\/strong>\u3002\u5916\u6765\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u907a\u4f1d\u5b50\u306f\u3001\u5b9f\u9a13\u8a66\u6599\u4e2d\u306b\u542b\u307e\u308c\u306a\u3044\u914d\u5217\u3092\u6709\u3059\u308b\u3001\u5408\u6210RNA\u307e\u305f\u306fin vitro\u8ee2\u5199RNA\u3067\u3059\u3002\u5916\u6765\u6027\u3067\u3042\u308b\u305f\u3081\u3001\u7570\u306a\u308b\u6761\u4ef6\u307e\u305f\u306f\u51e6\u7406\u306b\u304a\u3044\u3066\u7d30\u80de\u5185\u306b\u751f\u3058\u308b\u53ef\u80fd\u6027\u306e\u3042\u308b\u751f\u7269\u5b66\u7684\u5909\u52d5\u306e\u5f71\u97ff\u306f\u53d7\u3051\u307e\u305b\u3093\u3002\u5916\u6765\u6027\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u3001\u5b9f\u9a13\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306e\u3088\u308a\u65e9\u3044\u6bb5\u968e\u306b\u304a\u3044\u3066\u6dfb\u52a0\u3059\u308b\u3068\u3001\u3088\u308a\u591a\u304f\u306e\u30b9\u30c6\u30c3\u30d7\u306b\u304a\u3051\u308b\u3070\u3089\u3064\u304d\u3092\u6a19\u6e96\u5316\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001\u5916\u6765\u6027\u306e\u8ee2\u5199\u7269\u3092\u7d30\u80de\u6eb6\u89e3\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6dfb\u52a0\u3059\u308b\u3068\u3001\u7d30\u80de\u6eb6\u89e3\u3001RNA\u7cbe\u88fd\u304a\u3088\u3073\u305d\u308c\u306b\u7d9a\u304fRT\u304a\u3088\u3073PCR\u53cd\u5fdc\u7528\u306e\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3068\u3057\u3066\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u5916\u6765\u6027\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u4f8b\u3068\u3057\u3066\u306f\u3001<strong>\u5149\u5408\u6210\u907a\u4f1d\u5b50\u306a\u3069\u690d\u7269\u6a5f\u80fd\u306b\u7279\u7570\u7684\u907a\u4f1d\u5b50\u306e\u3001in 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