{"id":7987,"date":"2021-05-21T13:51:28","date_gmt":"2021-05-21T04:51:28","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=7987"},"modified":"2024-04-17T12:04:08","modified_gmt":"2024-04-17T03:04:08","slug":"frozen_sample_workshop_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/frozen_sample_workshop_ts_1\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u51cd\u7d50\u30b5\u30f3\u30d7\u30eb\u3001\u878d\u89e3\u5f8c\u306b\u3057\u3063\u304b\u308a\u6df7\u305c\u306a\u3044\u3068\u3053\u3093\u306a\u306b\u5dee\u304c\u51fa\u308b\uff01"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>DNA\u3084RNA\u306e\u30b5\u30f3\u30d7\u30eb\u306f\u51cd\u7d50\u4fdd\u5b58\u304c\u63a8\u5968\u3055\u308c\u307e\u3059\u304c\u3001\u4f7f\u7528\u3059\u308b\u3068\u304d\u306b\u306f\u878d\u304b\u3059\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u5f53\u793e\u3067\u958b\u50ac\u3057\u3066\u3044\u308b<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/lifescience-training.html?CID=gsd_pcr_sbu_r04_jp_cp1447_pjt7323_gsd00000_0so_blg_op_awa_og_s00_ws6_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0<\/a>\u3067\u3082\u3001DNA\u3084RNA\u6eb6\u6db2\u3092\u878d\u304b\u3057\u305f\u3068\u304d\u306b\u306f\u3057\u3063\u304b\u308a\u3068\u6df7\u5408\u3059\u308b\u3088\u3046\u306b\u304a\u4f1d\u3048\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u305f\u3060\u878d\u304b\u3057\u305f\u3060\u3051\u3067\u306f\u6eb6\u6db2\u4e2d\u306b\u6fc3\u5ea6\u306e\u3080\u3089\u304c\u751f\u3058\u308b\u3068\u8003\u3048\u3089\u308c\u3001\u7cbe\u5ea6\u306e\u9ad8\u3044\u30c7\u30fc\u30bf\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u306a\u304f\u306a\u308b\u305f\u3081\u3067\u3059\u3002<br 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Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays\/taqman-gene-expression.html?CID=gsd_pcr_sbu_r04_jp_cp1447_pjt7323_gsd00000_0so_blg_op_awa_og_s00_ws6_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">TaqMan Gene Expression Assays<\/a>(GAPDH\uff09<br \/>\n\u30fbApplied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/quantstudio-3-5-real-time-pcr-system\/quantstudio-5.html?CID=gsd_pcr_sbu_r04_jp_cp1447_pjt7323_gsd00000_0so_blg_op_awa_og_s00_ws6_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">QuantStudio\u2122 5 \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0<\/a><\/p>\n<p>\u65b9\u6cd5\uff1a<br \/>\n\u30fb\u30d5\u30ea\u30fc\u30b6\u30fc\u3067\u5341\u5206\u306b\u51cd\u7d50\u3057\u305fcDNA\u30b5\u30f3\u30d7\u30eb30 \u00b5L\uff08\u30c1\u30e5\u30fc\u30d62\u672c\uff09\u3092\u3001\u5ba4\u6e29\u3067\u9759\u7f6e\u3057\u3066\u89e3\u51cd\u3002<br \/>\n\u30fb1\u672c\u306e\u30c1\u30e5\u30fc\u30d6\u306f\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u30672\u79d2\u9593\u3057\u3063\u304b\u308a\u3068\u6df7\u5408\u3057\u3066Sample 1\u3068\u3057\u305f\u3002<br \/>\n\u30fb\u3082\u30461\u672c\u306e\u30c1\u30e5\u30fc\u30d6\u306f\u3001\u9759\u7f6e\u3057\u305f\u307e\u307e\u8868\u5c64\u90e8\uff08Sample 2\uff09\u3001\u4e2d\u5c64\u90e8\uff08Sample 3\uff09\u3001\u5e95\u5c64\u90e8\uff08Sample 4\uff09\u304b\u30896.6 \u00b5L\u305a\u3064\u5206\u53d6\u3057\u3066\u5404\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u305f\uff08\u56f31\uff09\u3002<br \/>\n\u30fb\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u53cd\u5fdc\u6db2\u306f\u5b9a\u6cd5\u306b\u5f93\u3044\u3001TaqMan Fast Advanced Master Mix\u3068TaqMan Gene Expression Assays\uff08GAPDH\uff09\u3092\u7528\u3044\u3066cDNA\u306f2 ng\/well\uff082 \u00b5L\/well\uff09\u3001\u53cd\u5fdc\u6db2\u91cf\u306f20 \u00b5L\/well\u3068\u306a\u308b\u3088\u3046\u306b\u8abf\u88fd\u3002<br \/>\n\u30fbQuantStudio 5 \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0\u3092\u4f7f\u7528\u3057\u3001n=3\u3067\u5b9f\u65bd\u3002<br \/>\n\u30fb\u5f97\u3089\u308c\u305fC<sub>T<\/sub>\u5024\u306e\u30c7\u30fc\u30bf\u304b\u3089\u5404\u30b5\u30f3\u30d7\u30eb\u306e\u5b9a\u91cf\u5024\u3092\u6982\u7b97\u3057\u305f\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-7988\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/10\/795316b92fc766b0181f6fef074f03fa.jpg\" alt=\"\" width=\"352\" height=\"294\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/10\/795316b92fc766b0181f6fef074f03fa.jpg 352w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/10\/795316b92fc766b0181f6fef074f03fa-300x251.jpg 300w\" sizes=\"auto, (max-width: 352px) 100vw, 352px\" \/><\/p>\n<h2>\u7d50\u679c<\/h2>\n<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u5897\u5e45\u66f2\u7dda\u3092\u898b\u308b\u3068\u3001\u3042\u304d\u3089\u304b\u306b\u5404\u30b5\u30f3\u30d7\u30eb\u9593\u3067\u305a\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\uff08\u56f32\uff09\u3002C<sub>T<\/sub>\u5024\u3092\u898b\u3066\u3082\u3001\u6700\u5927\u3067\u304a\u3088\u305d2\u7a0b\u5ea6\u306e\u5dee\u304c\u3042\u308a\u307e\u3057\u305f\uff08\u8868\uff09\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-7990\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/10\/2b530e80c7d0de90885e285c5d798063.jpg\" alt=\"\" width=\"638\" height=\"455\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/10\/2b530e80c7d0de90885e285c5d798063.jpg 638w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/10\/2b530e80c7d0de90885e285c5d798063-300x214.jpg 300w\" sizes=\"auto, (max-width: 638px) 100vw, 638px\" \/><br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-9491 size-full\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/05\/Table1-e1621572365240.jpg\" alt=\"\" width=\"600\" height=\"247\" \/><\/p>\n<p>\u5404\u30b5\u30f3\u30d7\u30eb\u306e\u5b9a\u91cf\u5024\u306e\u7b97\u51fa\u306f\u3001\u3057\u3063\u304b\u308a\u3068\u6df7\u5408\u3057\u305fSample 1\u3092\u57fa\u6e96\u50241\u3068\u3057\u3066\u3001C<sub>T<\/sub>\u50241\u306e\u5dee\u304c2\u500d\u91cf\u3067\u3042\u308b\u3068\u3057\u3066\u8a08\u7b97\u3057\u307e\u3057\u305f\uff08\u56f33\uff09\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-7992\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/10\/c8856789ec11ab8b1013037cef6929f9-1.jpg\" alt=\"\" width=\"486\" height=\"514\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/10\/c8856789ec11ab8b1013037cef6929f9-1.jpg 486w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2020\/10\/c8856789ec11ab8b1013037cef6929f9-1-284x300.jpg 284w\" sizes=\"auto, (max-width: 486px) 100vw, 486px\" \/><br \/>\n\u7d50\u679c\u3068\u3057\u3066\u3001\u3088\u304f\u6df7\u5408\u3057\u306a\u3044\u30681\u672c\u306e\u30c1\u30e5\u30fc\u30d6\u5185\u3067\u3082\u5927\u304d\u304f\u6fc3\u5ea6\u5dee\u304c\u751f\u3058\u3001\u8868\u5c64\u90e8\u306f\u8584\u304f\u3001\u5e95\u90e8\u304c\u9ad8\u6fc3\u5ea6\u306b\u306a\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u5b9a\u91cf\u5024\u306b\u63db\u7b97\u3059\u308b\u3068\u3001\u6700\u5927\u30674.1\u500d\uff08Sample 2\uff1a\u8868\u5c64\u3068Sample 4\uff1a\u5e95\u5c64\uff09\u3082\u306e\u5dee\u304c\u751f\u3058\u3066\u3057\u307e\u3044\u307e\u3057\u305f\u3002\u3057\u3063\u304b\u308a\u3068\u6df7\u5408\u3057\u305fSample 1\u3068\u6bd4\u8f03\u3057\u3066\u3082\u30011.4\uff08Sample 4\uff1a\u5e95\u5c64\uff09\uff5e2.9\u500d\uff08Sample 2\uff1a\u8868\u5c64\uff09\u306e\u5dee\u304c\u898b\u3089\u308c\u307e\u3057\u305f\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u3044\u304b\u304c\u3067\u3057\u305f\u3067\u3057\u3087\u3046\u304b\u3002<br \/>\nDNA\u3084RNA\u3001\u3082\u3057\u304f\u306f\u305d\u306e\u4ed6\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u3082\u3001\u51cd\u7d50\u3059\u308b\u3053\u3068\u306f\u9577\u671f\u4fdd\u5b58\u3059\u308b\u305f\u3081\u306b\u975e\u5e38\u306b\u6709\u52b9\u306a\u65b9\u6cd5\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u305f\u3060\u878d\u304b\u3059\u3060\u3051\u3067\u306f\u6fc3\u5ea6\u306b\u3080\u3089\u304c\u3067\u304d\u3066\u3057\u307e\u3044\u3001\u305b\u3063\u304b\u304f\u306e\u5b9f\u9a13\u7d50\u679c\u304c\u6b63\u3057\u304f\u5f97\u3089\u308c\u306a\u3044\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u307e\u305f\u30b5\u30f3\u30d7\u30eb\u3060\u3051\u306a\u304f\u3001\u51cd\u7d50\u4fdd\u5b58\u3057\u3066\u3044\u308b\u53cd\u5fdc\u8a66\u85ac\u3067\u3082\u540c\u69d8\u306b\u51cd\u7d50\u878d\u89e3\u306f\u6fc3\u5ea6\u306e\u3080\u3089\u304c\u3067\u304d\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u878d\u89e3\u3057\u305f\u6eb6\u6db2\u306f\u3001\u3057\u3063\u304b\u308a\u3068\u6df7\u5408\u3057\u3066\u304b\u3089\u304a\u4f7f\u3044\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u5f53\u793e\u3067\u306fRNA\u62bd\u51fa\u3084\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3001\u7d30\u80de\u57f9\u990a\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306a\u3069\u3001\u5b9f\u969b\u306b\u5b9f\u9a13\uff08\u5b9f\u7fd2\uff09\u3092\u884c\u3044\u3064\u3064\u5b66\u3079\u308b<a 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