{"id":806,"date":"2017-07-04T00:00:00","date_gmt":"2017-07-04T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic48\/"},"modified":"2025-04-16T15:10:02","modified_gmt":"2025-04-16T06:10:02","slug":"qpcr-basic48","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic48\/","title":{"rendered":"\u7d76\u5bfe\u5b9a\u91cf\u3068\u6bd4\u8f03\u5b9a\u91cf\u306e\u9055\u3044\u3068\u306f\uff1f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u4e3b\u306a4\u3064\u306e\u30c7\u30fc\u30bf\u89e3\u6790\u65b9\u6cd5"},"content":{"rendered":"<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u304a\u3051\u308b\u9069\u6b63\u306a\u5b9a\u91cf\u6cd5\u306f\u5b9f\u9a13\u306e\u76ee\u7684\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u7d76\u5bfe\u5b9a\u91cf\u6cd5\u306b\u3088\u3063\u3066\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5b9f\u969b\u306e\u30b3\u30d4\u30fc\u6570\u304c\u6c7a\u5b9a\u3055\u308c\u307e\u3059\u304c\u3001\u7d76\u5bfe\u5b9a\u91cf\u6cd5\u306f\u6700\u3082\u7169\u96d1\u3067\u56f0\u96e3\u306a\u5b9a\u91cf\u65b9\u6cd5\u3067\u3059\u3002\u3053\u306e\u65b9\u6cd5\u306b\u306f\u5468\u5230\u306a\u8a08\u753b\u3068\u9ad8\u5ea6\u306b\u6b63\u78ba\u306a\u691c\u91cf\u7dda\u4f5c\u6210\u304c\u5fc5\u8981\u3067\u3059\u3002\u7d76\u5bfe\u5b9a\u91cf\u306f\u30a6\u30a4\u30eb\u30b9\u4fa1\u306e\u6c7a\u5b9a\u306b\u591a\u304f\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n<li>\u6bd4\u8f03\u5b9a\u91cf\u6cd5\u306b\u304a\u3044\u3066\u3082\u7dbf\u5bc6\u306a\u5b9f\u9a13\u8a08\u753b\u304c\u5fc5\u8981\u3067\u3059\u304c\u3001\u5f97\u3089\u308c\u308b\u30c7\u30fc\u30bf\u306f\u6b63\u78ba\u306a\u30b3\u30d4\u30fc\u6570\u3067\u306f\u306a\u304f\u30b5\u30f3\u30d7\u30eb\u9593\u306e\u76f8\u5bfe\u5024\u306b\u306a\u308a\u307e\u3059\u3002\u907a\u4f1d\u5b50\u767a\u73fe\u7814\u7a76\u306b\u306f\u3053\u306e\u65b9\u6cd5\u304c\u3088\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u6bd4\u8f03\u5b9a\u91cf\u6cd5\u306b\u306f\u3001\u0394\u0394Ct\u6cd5\u3068\u691c\u91cf\u7dda\u6cd5\u306e2\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u3002<\/li>\n<\/ul>\n<p>\u4eca\u56de\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u4e3b\u306a4\u3064\u306e\u30c7\u30fc\u30bf\u89e3\u6790\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\u7d76\u5bfe\u5b9a\u91cf<\/h2>\n<p>\u7d76\u5bfe\u5b9a\u91cf\u6cd5\u306f\u3001\u7814\u7a76\u5bfe\u8c61\u3067\u3042\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5b9f\u969b\u306e\u30b3\u30d4\u30fc\u6570\u3092\u6c7a\u5b9a\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u306a\u7814\u7a76\u8005\u306b\u3068\u3063\u3066\u306f\u6700\u9069\u306a\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u89e3\u6790\u6cd5\u3067\u3059\u3002\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3046\u305f\u3081\u306b\u306f\u3001\u6fc3\u5ea6\u65e2\u77e5\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304b\u3089\u6570\u6841\u306e\u5e0c\u91c8\u7cfb\u5217\u3092\u4f5c\u6210\u3057\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u3088\u3063\u3066\u5897\u5e45\u3055\u305b\u3001\u5404\u30bf\u30fc\u30b2\u30c3\u30c8\u6fc3\u5ea6\u3092PCR\u7d50\u679c\u306eCt \u5024\u306b\u5bfe\u3057\u3066\u30d7\u30ed\u30c3\u30c8\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u672a\u77e5\u8a66\u6599\u306eCt \u5024\u3068\u3053\u306e\u691c\u91cf\u7dda\u304b\u3089\u3001\u30b3\u30d4\u30fc\u6570\u3092\u6c7a\u5b9a\u3057\u307e\u3059\u3002<\/p>\n<h3>\u691c\u91cf\u7dda\u306e\u4f5c\u6210\u306e\u6982\u8981<\/h3>\n<p>\u7d76\u5bfe\u5b9a\u91cf\u3067\u4f7f\u7528\u3059\u308b\u691c\u91cf\u7dda\u3067\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u30b3\u30d4\u30fc\u6570\u304c\u65e2\u77e5\u3067\u3042\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u691c\u91cf\u7dda\u306e\u4f5c\u6210\u306b\u5148\u7acb\u3063\u3066\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304c\u6b63\u78ba\u306b\u5b9a\u91cf\u3055\u308c\u308b\u3053\u3068\u304c\u8981\u6c42\u3055\u308c\u307e\u3059\u304c\u3001\u3053\u308c\u304c\u691c\u91cf\u7dda\u8a2d\u5b9a\u306e\u6700\u3082\u91cd\u8981\u306a\u70b9\u3068\u306a\u308a\u307e\u3059\u3002\u56f31\u3067\u306f\u691c\u91cf\u7dda\u306e\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u306b\u3064\u3044\u3066\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u8a66\u6599\u4e2d\u306b\u6b63\u78ba\u306b2 \u00d7 10<sup>11<\/sup>\u30b3\u30d4\u30fc\u5b58\u5728\u3059\u308b\u3068\u3057\u307e\u3059\u3002\u8a66\u6599\u306f10\u500d\u5e0c\u91c8\u30928\u56de\u6bb5\u968e\u5e0c\u91c8\u3057\u30012 \u00d7 10<sup>3<\/sup>\u30b3\u30d4\u30fc\u307e\u3067\u5e0c\u91c8\u3057\u307e\u3059\u3002\u5404\u5e0c\u91c8\u6db2\u306b\u5bfe\u3057\u3066\u5c11\u306a\u304f\u3068\u30823 \u53cd\u5fa9\u3067\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u5b9f\u884c\u3057\u307e\u3059\u3002\u5f97\u3089\u308c\u308b\u691c\u91cf\u7dda\u306b\u3088\u3063\u3066\u3001\u5404\u30b3\u30d4\u30fc\u6570\u3068\u305d\u308c\u305e\u308c\u306eCt\u5024\u3068\u306e\u76f8\u95a2\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u3053\u306e\u691c\u91cf\u7dda\u3068\u306e\u6bd4\u8f03\u306b\u3088\u308a\u3001\u672a\u77e5\u8a66\u6599\u306e\u30b3\u30d4\u30fc\u6570\u304c\u5c0e\u304d\u51fa\u3055\u308c\u307e\u3059\u3002\u5b9a\u91cf\u306e\u7cbe\u5ea6\u306f\u691c\u91cf\u7dda\u306e\u54c1\u8cea\u306b\u76f4\u63a5\u95a2\u9023\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<div id=\"attachment_2130\" style=\"width: 761px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig1.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2130\" class=\"size-full wp-image-2130\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig1.png\" alt=\"qpcr-basic48-fig1\" width=\"751\" height=\"476\" \/><\/a><p id=\"caption-attachment-2130\" class=\"wp-caption-text\">\u56f31 \u7d76\u5bfe\u5b9a\u91cf\u5411\u3051\u691c\u91cf\u7dda\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u306e\u305f\u3081\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/p><\/div>\n<p>\u7d76\u5bfe\u5b9a\u91cf\u306b\u304a\u3044\u3066\u306f\u4ee5\u4e0b\u306e\u70b9\u306b\u7559\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<ol>\n<li style=\"list-style-type: none\">\n<ol>\n<li>\u691c\u91cf\u7dda\u4f5c\u6210\u7528\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u6574\u3001\u304a\u3088\u3073\u305d\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u91cf\u3092\u6b63\u78ba\u306b\u5b9a\u91cf\u3057\u3066\u304a\u304f\u3053\u3068\u306f\u3001\u5b9f\u9a13\u306e\u57fa\u790e\u3068\u306a\u308a\u307e\u3059\u3002\u307e\u305f\u6bb5\u968e\u5e0c\u91c8\u306b\u304a\u3051\u308b\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u6b63\u78ba\u3055\u306f\u6975\u3081\u3066\u91cd\u8981\u3067\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306f\u975e\u5e38\u306b\u9ad8\u611f\u5ea6\u3067\u3042\u308b\u305f\u3081\u3001\u4e9b\u7d30\u306a\u4eba\u70ba\u7684\u30a8\u30e9\u30fc\u3067\u3082\u7d50\u679c\u306b\u5f71\u97ff\u3057\u3066\u3057\u307e\u3046\u5834\u5408\u3082\u3042\u308b\u306e\u3067\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u30bf\u30fc\u30b2\u30c3\u30c8\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304a\u3088\u3073\u5b9f\u969b\u306e\u8a66\u6599\u306e\u6bb5\u968e\u5e0c\u91c8\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3044\u3066\u3001RT\u52b9\u7387\u53ca\u3073PCR\u52b9\u7387\u304c\u307b\u307c\u4e00\u81f4\u3059\u308b\u3053\u3068\u306f\u975e\u5e38\u306b\u91cd\u8981\u3067\u3059\u3002<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<h3>\u691c\u91cf\u7dda\u4f5c\u6210\u306e\u305f\u3081\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u9078\u629e<\/h3>\n<p>\u5148\u8ff0\u3057\u305f\u3088\u3046\u306b\u3001\u7d76\u5bfe\u5b9a\u91cf\u306e\u305f\u3081\u306e\u691c\u91cf\u7dda\u4f5c\u6210\u306b\u3069\u306e\u3088\u3046\u306a\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3092\u4f7f\u7528\u3059\u308b\u304b\u306b\u3088\u308a\u3001\u30c7\u30fc\u30bf\u306e\u6b63\u78ba\u3055\u304c\u6c7a\u5b9a\u3055\u308c\u307e\u3059\u3002\u691c\u91cf\u7dda\u4f5c\u6210\u7528\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u4e2d\u306e\u30b3\u30d4\u30fc\u6570\u306e\u78ba\u8a8d\u306b\u306f\u3001\u5747\u4e00\u3067\u7d14\u5ea6\u306e\u9ad8\u3044\u72b6\u614b\u306e\u3082\u306e\u3092\u5229\u7528\u3055\u308c\u308b\u3068\u601d\u3044\u307e\u3059\u304c\u3001\u691c\u91cf\u7dda\u4f5c\u6210\u6642\u306b\u306f\u3001\u691c\u67fb\u5bfe\u8c61\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u72b6\u614b\u3068\u3067\u304d\u308b\u3060\u3051\u540c\u30ec\u30d9\u30eb\u306e\u7d14\u5ea6\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3092\u7528\u3044\u308b\u3053\u3068\u304c\u671b\u307e\u3057\u3044\u3067\u3059\u3002\u6838\u9178\u306e\u5358\u96e2\u304a\u3088\u3073\u9006\u8ee2\u5199\u306a\u3069\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u91cf\u306e\u5909\u5316\u304c\u8d77\u304d\u5f97\u308b\u305f\u3081\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306f\u3001\u5b9f\u9a13\u8a66\u6599\u304c\u95a2\u4e0e\u3059\u308b\u306e\u3068\u307b\u307c\u540c\u69d8\u306e\u53cd\u5fdc\u30b9\u30c6\u30c3\u30d7\u306b\u95a2\u4e0e\u3059\u308b\u3053\u3068\u3068\u306a\u308a\u307e\u3059\u3002\u4ee5\u4e0b\u306e\u30bf\u30a4\u30d7\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304c\u7d76\u5bfe\u5b9a\u91cf\u306e\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u3057\u3066\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<ol>\n<li><strong>DNA\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9<\/strong>\uff1a\u8208\u5473\u5bfe\u8c61\u30bf\u30fc\u30b2\u30c3\u30c8\u306ePCR\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u307e\u305f\u306f\u8208\u5473\u5bfe\u8c61\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u542b\u3080\u30d7\u30e9\u30b9\u30df\u30c9\u30af\u30ed\u30fc\u30f3\u3002\n<ul>\n<li>\u5229\u70b9\uff1a\u751f\u6210\u3001\u5b9a\u91cf\u304c\u5bb9\u6613\u3067\u3001\u9069\u5207\u306a\u4fdd\u5b58\u306b\u3088\u308a\u5bb9\u6613\u306b\u5b89\u5b9a\u6027\u3092\u7dad\u6301\u3067\u304d\u308b\u3002<\/li>\n<li>\u6b20\u70b9\uff1a\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306eRT\u306e\u92f3\u578b\u3068\u3057\u3066\u4f7f\u7528\u3067\u304d\u306a\u3044\u305f\u3081\u3001RT\u52b9\u7387\u306e\u5dee\u3092\u8a55\u4fa1\u3067\u304d\u306a\u3044\u3002<\/li>\n<\/ul>\n<\/li>\n<li>RNA \u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\uff1a\u30bf\u30fc\u30b2\u30c3\u30c8\u306ein vitro \u8ee2\u5199RNA\uff08\u56f32\uff09\n<ul>\n<li>\u5229\u70b9\uff1aRT\u52b9\u7387\u3092\u8a55\u4fa1\u3067\u304d\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u6700\u3082\u826f\u3044\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u306a\u308b\u3002<\/li>\n<li>\u6b20\u70b9\uff1a\u751f\u6210\u306b\u6642\u9593\u304c\u304b\u304b\u308a\u3001\u307e\u305f\u4e0d\u5b89\u5b9a\u3067\u3042\u308b\u305f\u3081\u9577\u671f\u4fdd\u5b58\u304c\u56f0\u96e3\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<div id=\"attachment_2131\" style=\"width: 736px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig2.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2131\" class=\"size-full wp-image-2131\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig2.png\" alt=\"qpcr-basic48-fig2\" width=\"726\" height=\"508\" \/><\/a><p id=\"caption-attachment-2131\" class=\"wp-caption-text\">\u56f32 in vitro \u8ee2\u5199\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u56f3\u89e3\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u304b\u3089\u751f\u6210\u3055\u308c\u305fPCR\u7523\u7269\u81ea\u8eab\u304c5\u2032 T7 \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u542b\u3080\u914d\u5217\u304a\u3088\u30733\u2032 poly\uff08T\uff09\u3092\u542b\u3080\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066\u5897\u5e45\u3055\u308c\u307e\u3059\u3002in vitro \u306e\u8ee2\u5199\u53cd\u5fdc\u306b\u3088\u308a\u30dd\u30ea\u30a2\u30c7\u30cb\u30eb\u5316\u3057\u305f\u30bb\u30f3\u30b9mRNA\u304c\u751f\u6210\u3055\u308c\u307e\u3059\u3002\u7cbe\u88fd\u5f8c\u3001\u6b63\u78ba\u306b\u5b9a\u91cf\u3057\u3001\u691c\u91cf\u7dda\u7528\u306b\u5e0c\u91c8\u3057\u307e\u3059\u3002<\/p><\/div>\n<p>\u5404RNA\u304a\u3088\u3073DNA\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306f\u5747\u8cea\u3067\u3042\u308b\u305f\u3081\u3001\u3057\u3070\u3057\u3070\u5b9f\u9a13\u8a66\u6599\u3088\u308a\u3082\u53cd\u5fdc\u52b9\u7387\u304c\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002\u3088\u308a\u5b9f\u9a13\u8a66\u6599\u306b\u8fd1\u3044\u4e0d\u5747\u8cea\u306a\u74b0\u5883\u3092\u5f62\u6210\u3057\u3066\u53cd\u5fdc\u52b9\u7387\u306e\u30d0\u30e9\u30f3\u30b9\u3092\u3068\u308b\u305f\u3081\u306b\u3001\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9RNA\u3068\u3057\u3066\u9175\u6bcdtRNA\u306a\u3069\u3092\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306b\u6dfb\u52a0\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9RNA\u306b\u3088\u308a\u3001cDNA\u5408\u6210\u901f\u5ea6\u309210\u500d\u307e\u3067\u6291\u5236\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<h3>\u691c\u91cf\u7dda\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3 &#8211; cRNA<\/h3>\n<p>\u7d76\u5bfe\u5b9a\u91cf\u306e\u305f\u3081\u306b\u6700\u9069\u306a\u691c\u91cf\u7dda\u306f\u3069\u306e\u3088\u3046\u306b\u4f5c\u6210\u3059\u308c\u3070\u3088\u3044\u304b\u3092\u793a\u3059\u305f\u3081\u3001\u3053\u306e\u30bb\u30af\u30b7\u30e7\u30f3\u3067\u306f\u3001\u7d76\u5bfe\u5b9a\u91cf\u6cd5\u306e\u305f\u3081\u306ecRNA\u691c\u91cf\u7dda\u306e\u4f5c\u6210\u3092\u30b9\u30c6\u30c3\u30d7\u6bce\u306b\u3054\u7d39\u4ecb\u3057\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n<p>T7 RNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u307e\u305f\u306fPCR\u7523\u7269\u3092\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u3057\u3066\u3001\u5bfe\u8c61\u907a\u4f1d\u5b50\u306e\u8ee2\u5199\u7523\u7269\u306e\u30d7\u30fc\u30eb\u3092\u4f5c\u88fd\u3059\u308b\u305f\u3081\u306b\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>cRNA\u306e\u6e2c\u5b9a\u306b\u306f\u3001UV\u5438\u53ce\u6e2c\u5b9a\u3088\u308a\u3082\u691c\u51fa\u9650\u754c\u306e\u5e45\u304c\u5e83\u304f\u7cbe\u5ea6\u304c\u3088\u308a\u9ad8\u3044\u86cd\u5149\u6e2c\u5b9a\u304c\u63a8\u5968\u3055\u308c\u307e\u3059\u3002\u30c7\u30b8\u30bf\u30ebPCR\u3092\u7528\u3044\u308b\u3068\u3001\u9ad8\u7cbe\u5ea6\u3067\u5b9a\u91cf\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u30b3\u30d4\u30fc\u6570\u3092\u7b97\u51fa\u3057\u305f\u5f8c\u3001\u691c\u51fa\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u306a\u3044\u9175\u6bcdtRNA\u3092cRNA\u3068tRNA\u306e\u6bd4\u304c1\uff1a100\u3068\u306a\u308b\u5272\u5408\u3067\u6dfb\u52a0\u3057\u3001\u751f\u4f53\u8a66\u6599\u7531\u6765\u306e\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3092\u518d\u73fe\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u3053\u306e\u6a19\u6e96\u6eb6\u6db2\u3092\u5c11\u306a\u304f\u3068\u30825\uff5e6\u6841\u306b\u308f\u305f\u3063\u3066\u5e0c\u91c8\u3057\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u3088\u308bCt\u5024\u306e\u6c7a\u5b9a\u306b\u4f7f\u7528\u3057\u307e\u3059\u3002<\/p>\n<p>\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u4e0d\u6b63\u78ba\u3055\u306f\u7d76\u5bfe\u5b9a\u91cf\u306e\u30c7\u30fc\u30bf\u306b\u6975\u3081\u3066\u5927\u304d\u306a\u5f71\u97ff\u3092\u53ca\u307c\u3057\u307e\u3059\u3002\u9069\u5207\u306a\u6ce8\u610f\u3092\u6255\u3046\u3053\u3068\u306b\u3088\u308a\u3053\u306e\u5f71\u97ff\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u3053\u3053\u306b\u793a\u3059\u30d7\u30ec\u30fc\u30c8\u30bb\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u306f\u30013\u7a2e\u985e\u306e\u7570\u306a\u308bcRNA\u6bb5\u968e\u5e0c\u91c8\u7cfb\u5217\u3092\u4f5c\u88fd\u3057\u3001\u305d\u308c\u305e\u308c\u306e\u5404\u5e0c\u91c8\u6bb5\u968e\u306b\u3064\u3044\u30662\u53cd\u5fa9\u3067\u5897\u5e45\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/p>\n<p>\u6bb5\u968e\u5e0c\u91c8\u6eb6\u6db2\u306e\u6fc3\u5ea6\u7bc4\u56f2\u306f\u3001\u5b9f\u9a13\u306b\u6e2c\u5b9a\u3059\u308b\u8a66\u6599\u4e2d\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u6fc3\u5ea6\u3092\u5168\u3066\u30ab\u30d0\u30fc\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001\u672a\u77e5\u306e\u8a66\u9a13\u8a66\u6599\u306b10\u30b3\u30d4\u30fc\u306e\u907a\u4f1d\u5b50\u3057\u304b\u542b\u307e\u308c\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308b\u5834\u5408\u3001\u6700\u5c0f\u30dd\u30a4\u30f3\u30c8\u304c100\u30b3\u30d4\u30fc\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u307e\u3067\u306e\u691c\u91cf\u7dda\u3067\u306f\u4e0d\u5341\u5206\u3067\u3059\u3002<\/p>\n<p>\u5404\u5e0c\u91c8\u6bb5\u968e\u306b\u95a2\u3057\u3066\u30016\u500b\u306eCt \u5024\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u6700\u5927\u3068\u6700\u5c0f\u306eCt \u5024\u3092\u9664\u5916\u3057\u3066\u3001\u6b8b\u308a\u306e4\u500b\u306eCt \u5024\u306e\u5e73\u5747\u3092\u7b97\u51fa\u3057\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u3053\u306e\u4f8b\u306710<sup>\u20134 <\/sup>\u306e\u5e0c\u91c8\u6bb5\u968e\u306b\u6ce8\u76ee\u3059\u308b\u3068\uff08\u56f33\uff09\u3001\u540c\u4e00\u8a66\u6599\u306eCt\u5024\u306b2\u30b5\u30a4\u30af\u30eb\u3082\u306e\u3070\u3089\u3064\u304d\u304c\u3042\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u3053\u306e\u3070\u3089\u3064\u304d\u306f\u3001\u7279\u5b9a\u306e\u30b3\u30d4\u30fc\u6570\u306b\u76f8\u5f53\u3059\u308b\u3053\u306e\u5e0c\u91c8\u6bb5\u968e\u306eCt \u5024\u3092\u5e73\u5747\u5024\u306e21.4\u3068\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u6700\u5c0f\u9650\u306b\u6291\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n<div id=\"attachment_2132\" style=\"width: 724px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig3.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2132\" class=\"size-full wp-image-2132\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig3.png\" alt=\"qpcr-basic48-fig3\" width=\"714\" height=\"710\" \/><\/a><p id=\"caption-attachment-2132\" class=\"wp-caption-text\">\u56f33 \u691c\u91cf\u7dda\u4f5c\u6210\u306e\u305f\u3081\u306e\u30d7\u30ec\u30fc\u30c8\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u3002\u30b5\u30a4\u30af\u30eb\u95be\u5024\uff08Ct\uff09\u306e\u5e73\u5747\u5024\u3092\u7b97\u51fa\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u53cd\u5fa9\u53cd\u5fdc\u304b\u3089\u5f97\u3089\u308c\u308b\u3001\u6700\u5927\u5024\u304a\u3088\u3073\u6700\u5c0f\u5024\u306f\u6392\u9664\u3055\u308c\u307e\u3059\u3002<\/p><\/div>\n<h2>\u6bd4\u8f03\u5b9a\u91cf<\/h2>\n<p>\u6bd4\u8f03\u5b9a\u91cf\u3082\u6280\u8853\u7684\u306b\u306f\u56f0\u96e3\u3067\u3059\u304c\u3001\u7d76\u5bfe\u5b9a\u91cf\u307b\u3069\u53b3\u683c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u6bd4\u8f03\u5b9a\u91cf\u306f\u591a\u304f\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u7814\u7a76\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u6280\u8853\u3067\u3042\u308a\u3001\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\uff08\u30ce\u30fc\u30de\u30eb\uff09\u8a66\u6599\u304a\u3088\u30731\u3064\u4ee5\u4e0a\u306e\u5b9f\u9a13\u8a66\u6599\u5b58\u5728\u4e0b\u3067\u3001\u8208\u5473\u5bfe\u8c61\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u306e\u5909\u52d5\u3092\u89e3\u6790\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u65b9\u6cd5\u306b\u304a\u3044\u3066\u306f\u6b63\u78ba\u306a\u30b3\u30d4\u30fc\u6570\u6c7a\u5b9a\u306f\u5fc5\u8981\u3067\u306f\u306a\u304f\u3001\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u8a66\u6599\u3068\u6bd4\u8f03\u3057\u305f\u76f8\u5bfe\u6bd4\u306b\u7126\u70b9\u3092\u5f53\u3066\u307e\u3059\u3002<\/p>\n<p>\u3053\u3053\u3067\u306f\u3001\u6bd4\u8f03\u5b9a\u91cf\u306e\u4e00\u822c\u7684\u306a\u65b9\u6cd5\u306e\u6982\u8981\u3092\u793a\u3057\u3001\u5404\u65b9\u6cd5\u306b\u304a\u3051\u308b\u3070\u3089\u3064\u304d\u3092\u6700\u5c11\u9650\u306b\u6291\u3048\u308b\u65b9\u6cd5\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h3>\u6bd4\u8f03\u5b9a\u91cf\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0 &#8211; \u0394Ct<\/h3>\n<p>\u3053\u308c\u306f\u6700\u3082\u57fa\u672c\u7684\u306a\u6bd4\u8f03\u5b9a\u91cf\u6cd5\u3067\u3059\u3002\u8208\u5473\u5bfe\u8c61\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u306b\u95a2\u3057\u3066\u3001\u8a66\u9a13\u8a66\u6599\u304a\u3088\u3073\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u8a66\u6599\u306e\u4e21\u8005\u304b\u3089Ct\u5024\u304c\u5f97\u3089\u308c\u3001\u305d\u308c\u3089\u306e\u5dee\u304c\u0394Ct\u3068\u306a\u308a\u307e\u3059\u3002\u76f8\u5bfe\u6bd4\u306f\u5358\u306b2\u306e\u0394Ct \u4e57\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>\u76f8\u5bfe\u6bd4 = 2<sup>\u0394Ct<\/sup><\/p>\n<p>\u3053\u306e\u57fa\u672c\u7684\u306a\u65b9\u6cd5\u306f\u3001\u8a66\u6599\u306e\u91cf\u3001\u54c1\u8cea\u3001\u307e\u305f\u306f\u53cd\u5fdc\u52b9\u7387\u306e\u88dc\u6b63\u3092\u884c\u3048\u306a\u3044\u3068\u3044\u3046\u70b9\u3067\u3001\u5b8c\u5168\u306a\u65b9\u6cd5\u3068\u306f\u8a00\u3048\u307e\u305b\u3093\uff08\u56f34\uff09\u3002<\/p>\n<div id=\"attachment_2133\" style=\"width: 733px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig4.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2133\" class=\"size-full wp-image-2133\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig4.png\" alt=\"qpcr-basic48-fig4\" width=\"723\" height=\"560\" \/><\/a><p id=\"caption-attachment-2133\" class=\"wp-caption-text\">\u56f34 \u51e6\u7406\u30b5\u30f3\u30d7\u30eb\u3068\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u306b\u304a\u3051\u308b\u767a\u73fe\u91cf\u306e\u76f8\u5bfe\u5b9a\u91cf\u3002\u51e6\u7406\u30b5\u30f3\u30d7\u30eb\u304a\u3088\u3073\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u30922\u53cd\u5fa9\u3067\u30e9\u30f3\u3057\u307e\u3057\u305f\u3002\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u306eCt \u5024\u306f6\u3067\u3042\u308a\u3001\u51e6\u7406\u30b5\u30f3\u30d7\u30eb\u306eCt \u5024\u306f12\u3067\u3057\u305f\u3002\u0394Ct \u6cd5\u306b\u3088\u308b\u3068\u3001\u51e6\u7406\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u306e\u76f8\u5bfe\u767a\u73fe\u91cf\u306e\u8a08\u7b97\u5024\u306f\u3001\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u3088\u308a64\u500d\u4f4e\u3044\u5024\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3092\u4f7f\u7528\u3057\u3066\u3044\u306a\u3044\u305f\u3081\u3001\u5b9f\u9a13\u306e\u3070\u3089\u3064\u304d\u304c\u3053\u306e\u7d50\u679c\u306b\u53ca\u307c\u3057\u3066\u3044\u308b\u5f71\u97ff\u304c\u672a\u77e5\u3067\u3042\u308a\u3001\u7d50\u679c\u306f\u4fe1\u983c\u6027\u304c\u3042\u308b\u3082\u306e\u3068\u306f\u307f\u306a\u3055\u308c\u307e\u305b\u3093\u3002<\/p><\/div>\n<h3>\u6bd4\u8f03\u5b9a\u91cf\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0 &#8211; \u0394\u0394Ct<\/h3>\n<p>\u0394\u0394Ct \u6cd5\u306f\u975e\u5e38\u306b\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u65b9\u6cd5\u3067\u3001\u5b9f\u9a13\u8a66\u6599\u304b\u3089\u306e\u7d50\u679c\u3092\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\uff08\u4f8b\uff1a\u672a\u51e6\u7406\u307e\u305f\u306f\u91ce\u751f\u578b\u8a66\u6599\uff09\u304a\u3088\u3073\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\uff08\u4f8b\uff1a\u30cf\u30a6\u30b9\u30ad\u30fc\u30d4\u30f3\u30b0\u907a\u4f1d\u5b50\u767a\u73fe\uff09\u306e\u4e21\u8005\u306e\u7d50\u679c\u3092\u6bd4\u8f03\u3057\u307e\u3059\u3002\u3053\u306e\u65b9\u6cd5\u3067\u306f\u3001\u8a66\u9a13\u8a66\u6599\u4e2d\u304a\u3088\u3073\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u8a66\u6599\u4e2d\u306e\u8208\u5473\u5bfe\u8c61\u907a\u4f1d\u5b50\uff08GOI\uff09\u306eCt\u304c\u3001\u540c\u30582\u3064\u306e\u8a66\u6599\u4e2d\u306e\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\uff08norm\uff09\u907a\u4f1d\u5b50\u306eCt\u306b\u95a2\u3057\u3066\u76f8\u5bfe\u6bd4\u8f03\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u5f97\u3089\u308c\u308b\u0394\u0394Ct \u5024\u304c\u767a\u73fe\u76f8\u5bfe\u6bd4\u306e\u6c7a\u5b9a\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002<\/p>\n<p>\u76f8\u5bfe\u6bd4 = 2<sup>\uff0d\u0394\u0394Ct<\/sup><br \/>\n\u0394Ct<sub>\u8a66\u9a13\u8a66\u6599<\/sub> \u2013 \u0394Ct<sub>\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc<\/sub> = \u0394\u0394Ct<br \/>\nCt<sub>GOI<\/sub><sup>S<\/sup> \u2013 Ct<sub>norm<\/sub><sup>S<\/sup> = \u0394Ct<sub>\u8a66\u9a13\u8a66\u6599<\/sub><br \/>\nCt<sub>GOI<\/sub><sup>C<\/sup> \u2013 Ct<sub>norm<\/sub><sup>C<\/sup> = \u0394Ct<sub>\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc<\/sub><\/p>\n<p>\u0394\u0394Ct \u6cd5\u3067\u306f\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u304a\u3088\u3073\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u306e\u53cd\u5fdc\u52b9\u7387\u304c\u540c\u7b49\u3067\u3042\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u3082\u3061\u308d\u3093\u3001\u8aa4\u5dee\u306e\u8a31\u5bb9\u7bc4\u56f2\u304c\u3069\u306e\u7a0b\u5ea6\u3067\u3042\u308b\u304b\u3068\u3044\u3046\u7591\u554f\u304c\u751f\u3058\u308b\u306e\u306f\u5f53\u7136\u3067\u3059\u3002\u305d\u306e\u6c7a\u5b9a\u306b\u306f\u3001\u540c\u4e00\u8a66\u6599\u3092\u4f7f\u7528\u3057\u305f\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u907a\u4f1d\u5b50\u304a\u3088\u3073\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u4e21\u65b9\u306e\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3059\u308b\u65b9\u6cd5\u304c\u7528\u3044\u3089\u308c\u307e\u3059\uff08\u56f35\uff09\u3002\u5404\u5e0c\u91c8\u30b5\u30f3\u30d7\u30eb\u306b\u95a2\u3057\u3066\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3068\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u9593\u306e\u5e73\u5747\u7684\u0394Ct\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u91cd\u8981\u306a\u306e\u306f\u5024\u305d\u306e\u3082\u306e\u3067\u306f\u306a\u304f\u3001\u5404\u5e0c\u91c8\u6bb5\u968e\u306b\u304a\u3051\u308b\u5024\u306e\u4e00\u8cab\u6027\u3067\u3059\u3002<\/p>\n<p>\u7814\u7a76\u306b\u3088\u3063\u3066\u306f\u3001\u52b9\u7387\u306b\u304a\u3051\u308b\u3053\u306e\u308f\u305a\u304b\u306a\u8aa4\u5dee\u304c\u4e0d\u6b63\u78ba\u3055\u306e\u539f\u56e0\u3068\u306a\u308b\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u3002\u8208\u5473\u5bfe\u8c61\u907a\u4f1d\u5b50\u304a\u3088\u3073\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u907a\u4f1d\u5b50\u306e\u4e21\u8005\u306b\u95a2\u3059\u308b\u88dc\u6b63\u3092\u884c\u3046\u3053\u3068\u306b\u3088\u308a\u3001\u5897\u5e45\u52b9\u7387\u306e\u3070\u3089\u3064\u304d\u306b\u3088\u308b\u52b9\u679c\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<div id=\"attachment_2134\" style=\"width: 749px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig5.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2134\" class=\"size-full wp-image-2134\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig5.png\" alt=\"qpcr-basic48-fig5\" width=\"739\" height=\"797\" \/><\/a><p id=\"caption-attachment-2134\" class=\"wp-caption-text\">\u56f35 \u76f8\u5bfe\u52b9\u7387\u30d7\u30ed\u30c3\u30c8\u3002\u0394Ct \u306e\u7bc4\u56f2\u306f2.0 \uff5e 2.5 \u3067\u3059\u3002\u0394Ct\u3092\u5e0c\u91c8\u5ea6\u307e\u305f\u306fRNA\u306e\u30a4\u30f3\u30d7\u30c3\u30c8\u91cf\u306b\u5bfe\u3057\u3066\u30d7\u30ed\u30c3\u30c8\u3057\u3001\u50be\u304d\u3092\u6c42\u3081\u307e\u3059\u3002\u5b8c\u5168\u306b\u5e73\u3089\u306a\u66f2\u7dda\uff08\u50be\u304d = 0\uff09\u306f\u3001\u3059\u3079\u3066\u306e\u30a4\u30f3\u30d7\u30c3\u30c8\u6fc3\u5ea6\u306b\u304a\u3051\u308b\u52b9\u7387\u304c\u540c\u7b49\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u3001\u0394\u0394Ct \u6cd5\u306b\u304a\u3044\u3066\u306f\u30010.1 \u672a\u6e80\u306e\u50be\u304d\u304c\u8a31\u5bb9\u7bc4\u56f2\u3067\u3042\u308b\u3068\u901a\u5e38\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p><\/div>\n<h3>\u6bd4\u8f03\u5b9a\u91cf\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0 &#8211; \u691c\u91cf\u7dda\u6cd5<\/h3>\n<p>\u6bd4\u8f03\u5b9a\u91cf\u306e\u691c\u91cf\u7dda\u6cd5\u306f\u3001\u8a66\u9a13\u8a66\u6599\u4e2d\u304a\u3088\u3073\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u8a66\u6599\u4e2d\u306b\u304a\u3051\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u306eCt\u306e\u5dee\u3092\u4f7f\u7528\u3057\u307e\u3059\u304c\u3001\u3053\u306e\u5024\u306f\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u907a\u4f1d\u5b50CtS\u306b\u5bfe\u3057\u3066\u6a19\u6e96\u5316\u3055\u308c\u3001\u5897\u5e45\u52b9\u7387\u306e\u308f\u305a\u304b\u306a\u3070\u3089\u3064\u304d\u306b\u5bfe\u3057\u3066\u3082\u88dc\u6b63\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u65b9\u6cd5\u306b\u304a\u3044\u3066\u306f\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u304a\u3088\u3073\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u306e\u4e21\u65b9\u306e\u5897\u5e45\u52b9\u7387\u3092\u6c7a\u5b9a\u3059\u308b\u305f\u3081\u306e\u691c\u91cf\u7dda\u304c\u5fc5\u8981\u3067\u3059\u304c\u3001\u524d\u8ff0\u306e\u65b9\u6cd5\u3067\u4f7f\u7528\u3055\u308c\u3066\u3044\u305f\u4eee\u5b9a\u3092\u6392\u9664\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u3053\u306e\u65b9\u6cd5\u306b\u304a\u3044\u3066\u306f\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u907a\u4f1d\u5b50\u304c\u3001\u89e3\u6790\u5bfe\u8c61\u306e\u8a66\u6599\u3059\u3079\u3066\u306b\u304a\u3044\u3066\u540c\u4e00\u3067\u3042\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<p>Fold difference =(E<sub>target<\/sub>)\u0394Ct<sub>target<\/sub>\/(E<sub>normalizer<\/sub>)\u0394Ct<sub>normalizer<\/sub><br \/>\nE = efficiency from standard curve E = 10<sup>[-1\/slope]<\/sup><br \/>\n\u0394Ct<sub>target<\/sub> = Ct<sub>GOI<\/sub><sup>C<\/sup> &#8211; Ct<sub>GOI<\/sub><sup>S<\/sup><br \/>\n\u0394Ct<sub>normalizer<\/sub> = Ct<sub>norm<\/sub><sup>C<\/sup>&#8211; Ct<sub>norm<\/sub><sup>S<\/sup><br \/>\n\uff08M.W. Pfaffl \u8457 A-Z of Quantitative PCR \u304b\u3089\u5f15\u7528\u3057\u305f\u76f8\u5bfe\u6bd4\u306b\u95a2\u3059\u308b\u7b49\u5f0f\u3002\uff09<\/p>\n<p>\u8a08\u7b97\u7528\u306b\u6700\u3082\u6b63\u78ba\u306a\u76f8\u5bfe\u5024\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\u3001\u691c\u91cf\u7dda\u306e\u305f\u3081\u306e\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u8a66\u6599\u3092\u6ce8\u610f\u6df1\u304f\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u306f\u3001\u5b9f\u9a13\u8a66\u6599\u3068\u540c\u3058\u7cbe\u88fd\u904e\u7a0b\u3067\u7cbe\u88fd\u3055\u308c\u3001\u540c\u3058\u53cd\u5fdc\u6761\u4ef6\u3067\u8abf\u6574\u3055\u308c\u305f\u3001\u5b9f\u9a13\u8a66\u6599\u3068\u540c\u7b49\u306e\u8907\u96d1\u6027\u3092\u6709\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u5b8c\u74a7\u306a\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u306f\u5bfe\u8c61\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u542b\u3080\u3001\u4e0d\u5747\u8cea\u306a\u8a66\u6599\u3067\u3042\u308a\u3001\u4f8b\u3048\u3070\u7814\u7a76\u4e2d\u306e\u7d30\u80de\u682a\u307e\u305f\u306f\u7d44\u7e54\u304b\u3089\u306e\u30c8\u30fc\u30bf\u30ebRNA\u306a\u3069\u304c\u542b\u307e\u308c\u307e\u3059\u3002\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u3068\u5b9f\u9a13\u8a66\u6599\u306e\u5dee\u306f\u3059\u3079\u3066\u4e0d\u6b63\u78ba\u306a\u52b9\u7387\u88dc\u6b63\u306b\u3064\u306a\u304c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u3001\u305d\u306e\u7d50\u679c\u3068\u3057\u3066\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u76f8\u5bfe\u6bd4\u306b\u95a2\u3057\u3066\u4e0d\u6b63\u78ba\u306a\u7d50\u679c\u304c\u7b97\u51fa\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3068\u3044\u3046\u3053\u3068\u306b\u7559\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30b3\u30d4\u30fc\u6570\u306f\u5b9f\u6e2c\u5024\u3067\u306f\u306a\u3044\u306e\u3067\u3001\u5e0c\u91c8\u307e\u305f\u306f\u5e0c\u91c8\u5024\u306f\u4efb\u610f\u306a\u5024\u306b\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\u691c\u91cf\u7dda\u6cd5\u306b\u304a\u3044\u3066\u3082\u3001\u0394\u0394Ct 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\u5024\u3092\u8abf\u6574\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u691c\u91cf\u7dda\u304b\u3089\u306e\u5897\u5e45\u52b9\u7387\u5024\uff08\u7406\u60f3\u7684\u306b\u306f\u540c\u4e00\u306e\u30d7\u30ec\u30fc\u30c8\u3067\u30e9\u30f3\u3055\u308c\u305f\u3082\u306e\uff09\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u52b9\u7387\u306f\u4e21\u8005\u306e\u50be\u304d\u304b\u3089\u5c0e\u304d\u51fa\u3055\u308c\u307e\u3059\uff08\u56f36\uff09\u3002<\/p>\n<p>\u4ee5\u4e0a\u3092\u8981\u7d04\u3059\u308b\u3068\u3001\u5b9a\u91cf\u6cd5\u306e\u9078\u629e\u306b\u304a\u3051\u308b\u7b2c\u4e00\u6bb5\u968e\u306f\u3001\u307e\u305a\u7d76\u5bfe\u5b9a\u91cf\u3068\u76f8\u5bfe\u5b9a\u91cf\u306e\u3044\u305a\u308c\u304c\u6700\u9069\u3067\u3042\u308b\u304b\u3092\u5224\u65ad\u3059\u308b\u3053\u3068\u3067\u3059\u3002\u8a66\u6599\u4e2d\u306b\u5b58\u5728\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u306e\u6570\u3092\u77e5\u308b\u5fc5\u8981\u304c\u3042\u308b\u5834\u5408\u306b\u306f\u3001\u65e2\u77e5\u91cf\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3092\u4f7f\u7528\u3057\u3066\u3001\u6b63\u78ba\u306a\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u5927\u90e8\u5206\u306e\u5b9f\u9a13\u306b\u304a\u3044\u3066\u306f\u3001\u76f8\u5bfe\u7684\u5b9a\u91cf\u6cd5\u304c\u9069\u5207\u306a\u65b9\u6cd5\u3068\u306a\u308a\u307e\u3059\u3002\u0394Ct\u6cd5\u3067\u306f\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3092\u4f7f\u7528\u3057\u307e\u305b\u3093\u304c\u3001\u0394\u0394Ct \u6cd5\u3067\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30d7\u30ed\u30bb\u30b9\u306b\u304a\u3051\u308b\u3070\u3089\u3064\u304d\u3092\u88dc\u6b63\u3059\u308b\u305f\u3081\u306b1\u3064\u4ee5\u4e0a\u306e\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u907a\u4f1d\u5b50\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u53cd\u5fdc\u52b9\u7387\u306e\u8abf\u6574\u3092\u542b\u3080\u3001\u3042\u308b\u3044\u306f\u542b\u307e\u306a\u3044\u5dee\u306e\u7b97\u51fa\u3092\u884c\u3046\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<div id=\"attachment_2135\" style=\"width: 737px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig6.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2135\" class=\"size-full wp-image-2135\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig6.png\" alt=\"qpcr-basic48-fig6\" width=\"727\" height=\"474\" \/><\/a><p id=\"caption-attachment-2135\" class=\"wp-caption-text\">\u56f36 \u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3068\u8208\u5473\u5bfe\u8c61\u907a\u4f1d\u5b50\u306eCt \u5024\u3092\u8abf\u6574\u3059\u308b\u305f\u3081\u306b\u3001\u691c\u91cf\u7dda\u304b\u3089\u5897\u5e45\u52b9\u7387\u5024\u304c\u5c0e\u304d\u51fa\u3055\u308c\u307e\u3059\u3002HeLa RNA\u30926\u30aa\u30fc\u30c0\u30fc\u306e\u7bc4\u56f2\u3067\u5e0c\u91c8\u3057\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u884c\u3063\u3066\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u304a\u3088\u3073\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\uff08GOI\uff09\u4e21\u8005\u306b\u95a2\u3059\u308b\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3057\u307e\u3057\u305f\u3002<\/p><\/div>\n<h2>\u9ad8\u5206\u89e3\u80fd\u878d\u89e3\u66f2\u7dda\uff08HRM\uff09\u89e3\u6790<\/h2>\n<p>\u9ad8\u5206\u89e3\u80fd\u878d\u89e3\u66f2\u7dda\uff08HRM\uff09\u89e3\u6790\u306f\u3001SNP\u3001\u65b0\u3057\u3044\u5909\u7570\u304a\u3088\u3073\u30e1\u30c1\u30eb\u5316\u30d1\u30bf\u30fc\u30f3\u3092\u540c\u5b9a\u3059\u308b\u305f\u3081\u306e\u65b0\u3057\u3044\u65b9\u6cd5\u3067\u3059\u3002\u3053\u306e\u65b9\u6cd5\u306f\u540c\u4e00\u6761\u4ef6\u3067PCR\u5f8c\u306e\u5bc6\u5c01\u30c1\u30e5\u30fc\u30d6\u3092\u7528\u3044\u3066\u884c\u3044\u307e\u3059\u3002HRM\u89e3\u6790\u306f\u5f93\u6765\u306e\u878d\u89e3\u66f2\u7dda\u6cd5\u3068\u6bd4\u8f03\u3057\u3066\u3088\u308a\u611f\u5ea6\u306e\u9ad8\u3044\u65b9\u6cd5\u3067\u3001\u4e8c\u672c\u9396\u306eDNA\u304c\u4e00\u672c\u9396\u306eDNA\u306b\u89e3\u96e2\u3059\u308b\u6e29\u5ea6\u3092\u30e2\u30cb\u30bf\u30fc\u3057\u307e\u3059\u3002\u3053\u306e\u6e29\u5ea6\u306f\u3001\u5897\u5e45\u7523\u7269\u306e\u878d\u70b9\uff08Tm\uff09\u3068\u3057\u3066\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u5149\u5b66\u7cfb\u304a\u3088\u3073\u6e29\u5ea6\u5236\u5fa1\u7cfb\u306e\u6a5f\u5668\u6027\u80fd\u306e\u30a2\u30c3\u30d7\u30b0\u30ec\u30fc\u30c9\u3068\u5171\u306b\u3001\u9ad8\u901f\u3067\u306e\u30c7\u30fc\u30bf\u56de\u53ce\u3068\u9ad8\u7cbe\u5ea6\u306e\u6e29\u5ea6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u304a\u3088\u3073\u5747\u4e00\u6027\u3092\u5b9f\u73fe\u3059\u308b\u305f\u3081\u306e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306e\u89e3\u6790\u6a5f\u80fd\u3092\u517c\u306d\u5099\u3048\u305f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u88c5\u7f6e\u304c\u5fc5\u8981\u3067\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/quantstudio-systems\/models\/quantstudio-12-flex.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr54_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">QuantStudio 12K Flex<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/viia-7-real-time-pcr-system.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr54_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">ViiA 7<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/7500-fast-real-time-pcr-system.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr54_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">7500 Fast<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr54_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">StepOnePlus \u304a\u3088\u3073StepOne Real-Time PCR System<\/a>\u306a\u3069\u3092HRM\u89e3\u6790\u3092\u884c\u3046\u3053\u3068\u306e\u53ef\u80fd\u306a\u88c5\u7f6e\u3068\u3057\u3066\u73fe\u5728\u8ca9\u58f2\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>HRM\u89e3\u6790\u306b\u304a\u3044\u3066\u306f\u3001\u7d0480\uff5e250\u5869\u57fa\u5bfe\u306e\u907a\u4f1d\u5b50\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u3092\u9ad8\u6a5f\u80fd\u306a\u4e8c\u672c\u9396DNA\uff08dsDNA\uff09\u7d50\u5408\u8272\u7d20\u3092\u542b\u3080\u53cd\u5fdc\u6db2\u5185\u3067PCR\u306b\u3088\u308a\u5897\u5e45\u3057\u307e\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4415440?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr54_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Applied Biosystems\u2122 MeltDoctor\u2122 HRM Master Mix<\/a> \u3067\u306f\u3001\u4e8c\u672c\u9396DNA\u306e\u5b58\u5728\u4e0b\u3001\u86cd\u5149\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u304c\u4f4e\u304f\u8f1d\u5ea6\u306e\u9ad8\u3044Invitrogen\u2122 SYTO\u2122 9 \u8272\u7d20\u306e\u71b1\u5b89\u5b9a\u578b\u3067\u3042\u308bMeltDoctor HRM Dye\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u300240\u30b5\u30a4\u30af\u30eb\u306e\u30e9\u30f3\u306e\u5f8c\u3001\u5897\u5e45\u7523\u7269\u3092\u30a2\u30cb\u30fc\u30eb\u3055\u305b\u86cd\u5149\u5ea6\u304c\u6700\u3082\u9ad8\u3044\u72b6\u614b\u306b\u30b9\u30bf\u30f3\u30d0\u30a4\u3055\u305b\u307e\u3059\u3002HRM\u89e3\u6790\u3092\u958b\u59cb\u3059\u308b\u3068\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u88c5\u7f6e\u306f\u3086\u3063\u304f\u308a\u3068\u6e29\u5ea6\u3092\u4e0a\u6607\u3055\u305b\u308b\u3068\u540c\u6642\u306b\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u304b\u3089\u306e\u86cd\u5149\u30c7\u30fc\u30bf\u3092\u8a18\u9332\u3057\u307e\u3059\u3002PCR\u7523\u7269\u304c\u5909\u6027\uff08\u307e\u305f\u306f\u878d\u89e3\uff09\u3057\u59cb\u3081\u308b\u3068\u3001\u86cd\u5149\u8272\u7d20\u304c\u653e\u51fa\u3055\u308c\u308b\u306b\u3064\u308c\u3066\u5897\u5e45\u7523\u7269\u306e\u86cd\u5149\u306f\u305d\u306eTm\u306b\u8fd1\u3065\u304f\u307e\u3067\u3086\u3063\u304f\u308a\u3068\u4f4e\u4e0b\u3057\u3066\u3044\u304d\u307e\u3059\u3002Tm\u306b\u6700\u3082\u8fd1\u304f\u306a\u308b\u3068\u3001\u8a66\u6599\u304c\u4e8c\u672c\u9396DNA\u304b\u3089\u4e00\u672c\u9396DNA\u3078\u3068\u5909\u5316\u3059\u308b\u306e\u306b\u3042\u308f\u305b\u3066\u86cd\u5149\u306e\u6025\u6fc0\u306a\u4f4e\u4e0b\u304c\u89b3\u5bdf\u3055\u308c\u307e\u3059\uff08\u56f37\uff09\u3002<\/p>\n<div id=\"attachment_2136\" style=\"width: 722px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig7.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2136\" class=\"size-full wp-image-2136\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic48-fig7.png\" alt=\"qpcr-basic48-fig7\" width=\"712\" height=\"466\" \/><\/a><p id=\"caption-attachment-2136\" class=\"wp-caption-text\">\u56f37 PCR\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306e\u878d\u89e3\u66f2\u7dda\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u306e\u7279\u6027\u3002\u500b\u5225\u306eDNA\u914d\u5217\u6bce\u306b\u7279\u5fb4\u7684\u306a\u878d\u89e3\u66f2\u7dda\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u304c\u5b58\u5728\u3057\u307e\u3059\u3002\u5909\u7570\u3092Tm\u306e\u30b7\u30d5\u30c8\u307e\u305f\u306f\u878d\u89e3\u66f2\u7dda\u306e\u5f62\u306e\u5909\u5316\u3068\u3057\u3066\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u5f93\u6765\u306e\u878d\u89e3\u66f2\u7dda\u89e3\u6790\u3068\u306f\u5bfe\u7167\u7684\u306b\u3001HRM\u3067\u306f\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u9055\u3044\u3092\u6709\u3059\u308b\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u9593\u306e\u8b58\u5225\u304c\u53ef\u80fd\u3067\u3059\u3002HRM\u6cd5\u306fdsDNA\u7d50\u5408\u8272\u7d20\u3092\u7528\u3044\u305f\u591a\u304f\u306e\u65b0\u3057\u3044\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306e\u53ef\u80fd\u6027\u3092\u5e83\u3052\u307e\u3059\u3002<\/p><\/div>\n<h3>HRM\u89e3\u6790\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/h3>\n<p>\u6700\u3082\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u3044\u308bHRM\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306f\u3001\u907a\u4f1d\u5b50\u30b9\u30ad\u30e3\u30cb\u30f3\u30b0\u3067\u3059\u3002\u907a\u4f1d\u5b50\u30b9\u30ad\u30e3\u30cb\u30f3\u30b0\u306f\u3001PCR\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306b\u304a\u3051\u308b\u672a\u77e5\u306e\u5909\u7570\u306e\u691c\u7d22\u3067\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u306b\u5148\u7acb\u3063\u3066\u3001\u3042\u308b\u3044\u306f\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u306e\u4ee3\u308f\u308a\u306b\u884c\u308f\u308c\u307e\u3059\u3002PCR\u7523\u7269\u4e2d\u306e\u5909\u7570\u304cHRM\u306b\u3088\u3063\u3066\u691c\u51fa\u3055\u308c\u308b\u306e\u306f\u3001\u5909\u7570\u304c\u3042\u308b\u3068DNA\u878d\u89e3\u66f2\u7dda\u306e\u5f62\u306b\u5909\u5316\u304c\u73fe\u308c\u308b\u305f\u3081\u3067\u3059\u3002\u30d8\u30c6\u30ed\u4e8c\u91cf\u4f53\u306eDNA\u8a66\u6599\u306f\u3001\u5897\u5e45\u3055\u308c\u878d\u89e3\u3059\u308b\u3068\u3001\u30db\u30e2\u63a5\u5408\u306e\u91ce\u751f\u578b\u307e\u305f\u306f\u5909\u7570\u578b\u8a66\u6599\u3068\u306f\u7570\u306a\u308b\u878d\u89e3\u66f2\u7dda\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n<p>HRM\u89e3\u6790\u306e\u4e00\u822c\u7684\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u306f\u4ee5\u4e0b\u306e\u3082\u306e\u304c\u542b\u307e\u308c\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u907a\u4f1d\u5b50\u30b9\u30ad\u30e3\u30cb\u30f3\u30b0\uff08\u5909\u7570\u691c\u7d22\uff09<\/li>\n<li>\u5909\u7570\u89e3\u6790<\/li>\n<li>1\u5869\u57fa\u591a\u578b\uff08SNP\uff09\u691c\u51fa<\/li>\n<li>\u30d8\u30c6\u30ed\u63a5\u5408\u6027\u7814\u7a76<\/li>\n<li>\u7a2e\u306e\u540c\u5b9a<\/li>\n<li>\u30e1\u30c1\u30eb\u5316\u89e3\u6790<\/li>\n<\/ul>\n<p>\u5f93\u6765\u3053\u308c\u3089\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u306f\u3001\u5404\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u56fa\u6709\u306e\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u304c\u5fc5\u8981\u3067\u3057\u305f\u304c\u3001\u305d\u308c\u3089\u306f\u9ad8\u4fa1\u3067\u3001\u30c7\u30b6\u30a4\u30f3\u306b\u6642\u9593\u304c\u304b\u304b\u308b\u4e0a\u306b\u67d4\u8edf\u6027\u306b\u6b20\u3051\u3066\u3044\u307e\u3057\u305f\u3002dsDNA\u7d50\u5408\u8272\u7d20\u3092\u4f7f\u7528\u3059\u308bHRM\u306f\u3001\u30d7\u30ed\u30fc\u30d6\u30d9\u30fc\u30b9\u306e\u89e3\u6790\u3068\u540c\u69d8\u306e\u6027\u80fd\u3092\u3001\u3088\u308a\u4f4e\u4fa1\u683c\u3067\u67d4\u8edf\u6027\u306e\u3042\u308b\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>HRM\u306b\u306f\u591a\u304f\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u304c\u5b58\u5728\u3057\u307e\u3059\u304c\u3001Tm \u5024\u306e\u308f\u305a\u304b\u306a\u30b7\u30d5\u30c8\u3092\u691c\u51fa\u3059\u308b\u5fc5\u8981\u306e\u3042\u308b\u3001\u5358\u4e00\u5869\u57fa\u30ec\u30d9\u30eb\u3067\u306e\u8b58\u5225\u304c\u6700\u3082\u56f0\u96e3\u3067\u3059\u3002<\/p>\n<h3>HRM\u89e3\u6790\u30b1\u30df\u30b9\u30c8\u30ea<\/h3>\n<p>\u7279\u5225\u306a\u88c5\u7f6e\u304a\u3088\u3073\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u52a0\u3048\u3066\u3001HRM\u89e3\u6790\u306b\u306f\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u9055\u3044\u3092\u6709\u3059\u308b\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306e\u878d\u70b9\u3092\u8b58\u5225\u3059\u308b\u80fd\u529b\u306e\u3042\u308bdsDNA\u7d50\u5408\u8272\u7d20\u304c\u5fc5\u8981\u3067\u3059\u3002HRM\u3067\u306e\u4f7f\u7528\u306b\u6210\u529f\u3057\u3066\u3044\u308b\u8272\u7d20\u306b\u306f\u3001\u4ee5\u4e0b\u306e\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<ul>\n<li>MeltDoctor\u2122 HRM Dye<\/li>\n<li>SYTO\u2122 9\u8272\u7d20<\/li>\n<li>LCGreen\u2122 and LCGreen\u2122 Plus+ reagents\uff08Idaho Technology\uff09<\/li>\n<li>EvaGreen\u2122 dye\uff08 Biotium Inc.\uff09<\/li>\n<\/ul>\n<h3>HRM\u89e3\u6790\u30a2\u30c3\u30bb\u30a4\u6210\u529f\u306e\u9375<\/h3>\n<ul>\n<li>\u6700\u9ad8\u306e\u611f\u5ea6\u3092\u9054\u6210\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u3092\u77ed\u304f\u3057\u307e\u3059\u3002\u9577\u3044\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u3068\u6bd4\u8f03\u3057\u3066\u3001100\u5869\u57fa\u5bfe\u7a0b\u5ea6\u306e\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u3067\u306f\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u9055\u3044\u3092\u6709\u3059\u308b\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306e\u878d\u70b9\u3092\u8b58\u5225\u3059\u308b\u3053\u3068\u304c\u3088\u308a\u5bb9\u6613\u3068\u306a\u308a\u307e\u3059\u3002<\/li>\n<li>PCR\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306e\u7279\u7570\u6027\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002\u30df\u30b9\u30d7\u30e9\u30a4\u30df\u30f3\u30b0\u7523\u7269\u304a\u3088\u3073\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u3042\u308b\u3068\u30c7\u30fc\u30bf\u306e\u89e3\u91c8\u304c\u8907\u96d1\u3068\u306a\u308a\u307e\u3059\u3002\u9ad8\u3044\u7279\u7570\u6027\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\u3001200nM\u672a\u6e80\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u3092\u4f7f\u7528\u3057\u3001MgCl2 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HRM Master Mix\u306a\u3069\u306e\u30df\u30c3\u30af\u30b9\u8a66\u85ac\u3092\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<h2>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u89e3\u6790<\/h2>\n<p>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u306f\u540c\u4e00\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u53cd\u5fdc\u306b\u304a\u3044\u3066\u8907\u6570\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u89e3\u6790\u3059\u308b\u6280\u8853\u3067\u3059\u3002\u5404\u30bf\u30fc\u30b2\u30c3\u30c8\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u7279\u7570\u7684\u306a\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u307e\u305f\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u306b\u7d50\u5408\u3057\u305f\u7279\u5b9a\u306e\u8272\u7d20\u306b\u3088\u308a\u8b58\u5225\u3057\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u306f\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u907a\u4f1d\u5b50\u3068\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u3092\u540c\u4e00\u306e\u53cd\u5fdc\u306b\u304a\u3044\u3066\u5897\u5e45\u3059\u308b\u76ee\u7684\u3067\u4f7f\u7528\u3057\u307e\u3059\u3002<\/p>\n<p>\u7406\u8ad6\u7684\u306b\u306f\u3001\u7279\u5b9a\u306e\u53cd\u5fdc\u4e2d\u306b\u304a\u3044\u3066\u5897\u5e45\u304c\u53ef\u80fd\u306a\u30bf\u30fc\u30b2\u30c3\u30c8\u6570\u306f\u3001\u4f7f\u7528\u3067\u304d\u308b\u5149\u5b66\u7684\u306b\u8b58\u5225\u53ef\u80fd\u306a\u8272\u7d20\u306e\u6570\u3001\u304a\u3088\u3073\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u88c5\u7f6e\u306b\u3088\u3063\u3066\u52b1\u8d77\u304a\u3088\u3073\u691c\u51fa\u304c\u53ef\u80fd\u306a\u8272\u7d20\u306e\u6570\u306b\u3088\u3063\u3066\u5236\u9650\u3055\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u5b9f\u969b\u306b\u306f\u305d\u306e\u4ed6\u306e\u5b9f\u9a13\u7684\u5236\u7d04\u3082\u5b58\u5728\u3057\u307e\u3059\u3002\u4f8b\u3048\u3070\u53cd\u5fdc\u4e2d\u306b\u304a\u3044\u3066\u3001\u7570\u306a\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u304c\u304a\u4e92\u3044\u306b\u53cd\u5fdc\u3057\u306a\u3044\u3053\u3068\u304c\u5fc5\u8981\u3067\u3042\u308a\u3001\u3053\u308c\u3089\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306fdNTP\u304a\u3088\u3073\u71b1\u5b89\u5b9a\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306a\u3069\u306ePCR\u6210\u5206\u3092\u5171\u7528\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u306f\u6700\u9069\u5316\u306b\u6642\u9593\u304c\u304b\u304b\u308a\u307e\u3059\u304c\u3001\u3044\u304f\u3064\u304b\u306e\u512a\u308c\u305f\u5229\u70b9\u304c\u5b58\u5728\u3057\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u5909\u52d5\u304c\u5c11\u306a\u304f\u3001\u4e00\u8cab\u6027\u304c\u9ad8\u3044\u3002\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3068\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u3092\u540c\u4e00\u30c1\u30e5\u30fc\u30d6\u5185\u3067\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u3053\u308c\u30892\u3064\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5225\u3005\u306e\uff08\u96a3\u308a\u5408\u3063\u3066\u3044\u308b\u5834\u5408\u3067\u3082\uff09\u30a6\u30a7\u30eb\u306b\u304a\u3044\u3066\u5897\u5e45\u3059\u308b\u3053\u3068\u306b\u8d77\u56e0\u3059\u308b\u3001\u30a6\u30a7\u30eb\u9593\u306e\u8aa4\u5dee\u304c\u6392\u9664\u3055\u308c\u307e\u3059\u3002<\/li>\n<li>\u4f7f\u7528\u3059\u308b\u8a66\u85ac\u306e\u91cf\u304c\u5c11\u306a\u304f\u3001\u30b3\u30b9\u30c8\u30c0\u30a6\u30f3\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u3067\u306f\u540c\u6570\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u89e3\u6790\u306b\u5fc5\u8981\u306a\u53cd\u5fdc\u304c\u5c11\u306a\u304f\u3066\u6e08\u307f\u307e\u3059\u3002<\/li>\n<li>\u3088\u308a\u9ad8\u3044\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u3002\u5404PCR\u30e9\u30f3\u304a\u3088\u3073\u5404\u8a66\u6599\u306b\u3064\u3044\u3066\u3088\u308a\u591a\u304f\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u89e3\u6790\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3059\u3002<\/li>\n<\/ul>\n<h3>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u6210\u529f\u306e\u9375<\/h3>\n<p>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u3092\u6210\u529f\u3055\u305b\u308b\u305f\u3081\u306b\u306f\u3001\u4ee5\u4e0b\u306b\u793a\u3059\u4e8b\u9805\u3092\u542b\u3081\u305f\u591a\u304f\u306e\u8981\u56e0\u3092\u8003\u616e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306e\u30c7\u30b6\u30a4\u30f3<\/li>\n<li>\u8a66\u85ac\u306e\u6700\u9069\u5316\uff08\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u91cf\u3001\u53cd\u5fdc\u6210\u5206\u304a\u3088\u3073\u86cd\u5149\u8272\u7d20\u5206\u5b50\u3068\u30af\u30a8\u30f3\u30c1\u30e3\u30fc\u306e\u7d44\u307f\u5408\u308f\u305b\u306a\u3069\u3092\u542b\u307f\u307e\u3059\uff09<\/li>\n<li>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u306e\u691c\u8a3c<\/li>\n<\/ul>\n<h3>\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306e\u30c7\u30b6\u30a4\u30f3<\/h3>\n<p>\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306e\u30c7\u30b6\u30a4\u30f3\u306f\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u306b\u304a\u3044\u3066\u6700\u3082\u91cd\u8981\u306a\u56e0\u5b50\u3067\u3059\u3002\u53cd\u5fdc\u306e\u8907\u96d1\u6027\u304c\u5897\u3059\u306b\u3064\u308c\u3066\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u304c\u30c0\u30a4\u30de\u30fc\u3068\u306a\u308b\u53ef\u80fd\u6027\u3001\u3042\u308b\u3044\u306f\u5404\u53cd\u5fdc\u6210\u5206\u306e\u7af6\u5408\u306b\u3088\u308a\u8907\u6570\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5897\u5e45\u304c\u5236\u9650\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u5897\u52a0\u3057\u307e\u3059\u3002\u7af6\u5408\u52b9\u679c\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u3001\u6027\u80fd\u3092\u6700\u5927\u9650\u306b\u6d3b\u304b\u3059\u305f\u3081\u306b\u7279\u306b\u91cd\u8981\u306a\u56e0\u5b50\u3092\u4ee5\u4e0b\u306b\u793a\u3057\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306f\u77ed\u304f\u3057\u307e\u3059\u300250\u5869\u57fa\u5bfe\u304b\u3089150\u5869\u57fa\u5bfe\u306e\u7bc4\u56f2\u306e\u90e8\u5206\u3092\u5897\u5e45\u3059\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u53cd\u5fdc\u52b9\u7387\u304c\u4e0a\u6607\u3057\u307e\u3059\u3002<\/li>\n<li>Tm\u5024\u304c\u304a\u4e92\u3044\u306b2\u00b0C\u4ee5\u5185\u306e\u7bc4\u56f2\u306b\u3042\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u307e\u3059\u3002\u3059\u3079\u3066\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u304c\u540c\u4e00\u6e29\u5ea6\u3067\u30a2\u30cb\u30fc\u30eb\u3059\u308b\u3068\u3044\u3046\u3053\u3068\u306b\u7559\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002Tm\u5024\u306e\u9055\u3044\u306f\u52b9\u7387\u306e\u30d0\u30a4\u30a2\u30b9\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002<\/li>\n<li>\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306e\u30c7\u30b6\u30a4\u30f3\u306b\u306fBLAST\u691c\u7d22\u3092\u884c\u3044\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u5bfe\u3059\u308b\u7279\u7570\u6027\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u4f7f\u7528\u3057\u3066\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u307e\u305f\u306f\u30d7\u30ed\u30fc\u30d6\u306b\u30c0\u30a4\u30de\u30fc\u5f62\u6210\u3092\u8d77\u3053\u3057\u3084\u3059\u3044\u914d\u5217\u304c\u306a\u3044\u304b\u3069\u3046\u304b\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u7121\u6599\u30d7\u30ed\u30b0\u30e9\u30e0\u306e\u4e00\u3064\u3067\u3042\u308b\u3001AutoDimer\uff08\u7c73\u56fd\u56fd\u7acb\u6a19\u6e96\u6280\u8853\u7814\u7a76\u6240\u306eP.M.Vallone\u8457\uff09\u306f\u7279\u5b9a\u306e\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u306b\u304a\u3051\u308b\u3059\u3079\u3066\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306b\u95a2\u3057\u3066\u3001\u3059\u3079\u3066\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u304a\u3051\u308b\u30c0\u30a4\u30de\u30fc\u5f62\u6210\u306e\u50be\u5411\u3092\u89e3\u6790\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u5f0a\u793e\u306e\u30a6\u30a7\u30d6\u30b5\u30a4\u30c8\u3067<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/thermo-scientific-web-tools\/multiple-primer-analyzer.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr54_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Multiple Primer Analyzer<\/a>\u3092\u3054\u5229\u7528\u3044\u305f\u3060\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<\/ul>\n<h3>\u8a66\u85ac\u306e\u6700\u9069\u5316<\/h3>\n<p>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u306b\u304a\u3044\u3066\u6975\u3081\u3066\u91cd\u8981\u306a\u554f\u984c\u306f\u3001\u5897\u5e45\u3055\u308c\u308b\u7570\u306a\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u9593\u3067\u306e\u8a66\u85ac\u306e\u7af6\u5408\u3067\u3059\u3002\u9ad8\u3044\u5897\u5e45\u52b9\u7387\u3092\u78ba\u4fdd\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u91cf\u3092\u6e1b\u3089\u3059\u304b\u3001\u3059\u3079\u3066\u306ePCR\u6210\u5206\u306e\u6fc3\u5ea6\u3092\u4e0a\u6607\u3055\u305b\u308b\u304b\u3001\u3042\u308b\u3044\u306f\u305d\u306e\u4e21\u65b9\u304c\u5fc5\u8981\u3067\u3059\u3002\u4e00\u822c\u7684\u306b\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u306e\u6700\u9069\u5316\u304b\u3089\u521d\u3081\u3066\u3044\u305f\u3060\u304f\u30b1\u30fc\u30b9\u304c\u591a\u3044\u3067\u3059\u3002<\/p>\n<h3>\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u304a\u3088\u3073\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u91cf<\/h3>\n<p>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u306b\u304a\u3044\u3066\u306f\u3059\u3079\u3066\u306e\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u304c\u540c\u6570\u5b58\u5728\u3059\u308b\u308f\u3051\u3067\u306f\u306a\u304f\u3001\u307e\u305f\u540c\u3058\u52b9\u7387\u3067\u5897\u5e45\u3059\u308b\u308f\u3051\u3067\u3082\u3042\u308a\u307e\u305b\u3093\u3002\u3088\u308a\u5897\u5e45\u3057\u3084\u3059\u3044\u3042\u308b\u3044\u306f\u3088\u308a\u5927\u91cf\u306b\u5b58\u5728\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u5bfe\u3059\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u6fc3\u5ea6\u3092\u5236\u9650\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u53cd\u5fdc\u6027\u3092\u540c\u30ec\u30d9\u30eb\u306b\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001\u03b2-\u30a2\u30af\u30c1\u30f3\uff08\u9ad8\u30b3\u30d4\u30fc\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\uff09\u3092\u5b58\u5728\u91cf\u306e\u5c11\u306a\u3044\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u3059\u308b\u3068\u3001\u03b2-\u30a2\u30af\u30c1\u30f3\u304c\u30b5\u30a4\u30af\u30eb\u306e\u521d\u671f\u306e\u6bb5\u968e\u3067\u3001\u5171\u7528\u3059\u308b\u53cd\u5fdc\u6210\u5206\u3092\u67af\u6e07\u3055\u305b\u3066\u3057\u307e\u3046\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u03b2-\u30a2\u30af\u30c1\u30f3\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u91cf\u3092\u6e1b\u5c11\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u5897\u5e45\u901f\u5ea6\u304c\u5236\u9650\u3055\u308c\u3001\u3088\u308a\u5b58\u5728\u91cf\u306e\u5c11\u306a\u3044\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u969c\u5bb3\u306e\u306a\u3044\u72b6\u614b\u3067\u5897\u5e45\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u3001\u3088\u308a\u5b58\u5728\u91cf\u306e\u591a\u3044\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u95a2\u3057\u3066\u306f\u3001Ct 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href=\"https:\/\/www.thermofisher.com\/order\/fluorescence-spectraviewer\/#!\/?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr54_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u86cd\u5149\u30b9\u30da\u30af\u30c8\u30eb\u30d3\u30e5\u30fc\u30a2\u30fc<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<h3>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u306e\u691c\u8a3c<\/h3>\n<p>\u30b7\u30f3\u30b0\u30eb\u30d7\u30ec\u30c3\u30af\u30b9\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u5834\u5408\u3068\u540c\u69d8\u306b\u3001\u30a2\u30c3\u30bb\u30a4\u306e\u30e9\u30f3\u306e\u524d\u306b\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u4e2d\u306e\u3059\u3079\u3066\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u53cd\u5fdc\u52b9\u7387\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u306b\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u691c\u8a3c\u904e\u7a0b\u306f\u5927\u304d\u304f2\u3064\u306e\u6bb5\u968e\u306b\u5206\u3051\u3089\u308c\u307e\u3059\u3002<\/p>\n<ol>\n<li>\u5404\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u3092\u691c\u8a3c\u3057\u3001\u500b\u3005\u306e\u52b9\u7387\u3092\u540c\u5b9a\u3057\u307e\u3059\u3002\u3053\u308c\u304c\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u30c7\u30b6\u30a4\u30f3\u306e\u521d\u671f\u306e\u8a55\u4fa1\u3067\u3059\u3002<\/li>\n<li>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u5168\u4f53\u306e\u6700\u9069\u5316\u3002<\/li>\n<\/ol>\n<p>\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u3068\u3063\u3066\u7406\u60f3\u7684\u306a\u30c7\u30b6\u30a4\u30f3\u304a\u3088\u3073\u6761\u4ef6\u3092\u6c7a\u5b9a\u3059\u308b\u305f\u3081\u306b\u3001\u500b\u3005\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u95a2\u3059\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u30bb\u30c3\u30c8\u3092\u8a55\u4fa1\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u6bb5\u968e\u5e0c\u91c8\u306e\u691c\u91cf\u7dda\u3092\u7528\u3044\u3066\u8a55\u4fa1\u3057\u307e\u3059\u3002100%\u306b\u8fd1\u3044\u52b9\u7387\u304c\u5f97\u3089\u308c\u308b\u3053\u3068\u3092\u76ee\u6a19\u3068\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5404\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306e\u7d44\u307f\u5408\u308f\u305b\u3092\u6a5f\u80fd\u7684\u306b\u8a55\u4fa1\u3057\u305f\u5f8c\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u306e\u6700\u9069\u5316\u306b\u9032\u3093\u3067\u304f\u3060\u3055\u3044\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u95a2\u3059\u308b\u30b7\u30f3\u30b0\u30ec\u30c3\u30af\u30b9\u306e\u6761\u4ef6\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u3067\u3042\u308b\u5834\u5408\u306b\u306f\u6700\u9069\u3068\u306a\u3089\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308b\u3053\u3068\u306b\u7559\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30d1\u30cd\u30eb\u3092\u69cb\u7bc9\u3059\u308b\u969b\u306b\u306f\u3001\u521d\u56de\u306e\u5b9f\u9a13\u306b\u304a\u3044\u3066\u3059\u3079\u3066\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u306e\u3067\u306f\u306a\u304f\u30011\u56de\u306b1\u500b\u305a\u3064\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u6dfb\u52a0\u3057\u3066\u3044\u304f\u3053\u3068\u304c\u8ce2\u660e\u3067\u3059\u3002<\/p>\n<p>\u540c\u69d8\u306e\u691c\u91cf\u7dda\u6cd5\u3092\u4f7f\u7528\u3057\u3066\u3001\u3059\u3079\u3066\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30d7\u30ed\u30fc\u30d6\u3092\u6df7\u5408\u3057\u3001\u5404\u5e0c\u91c8\u6bb5\u968e\u306b\u95a2\u3057\u3066\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u3092\u5b9f\u884c\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5404\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u95a2\u3057\u3066\u5f97\u3089\u308c\u305f\u52b9\u7387\u3092\u3001\u5bfe\u5fdc\u3059\u308b\u30b7\u30f3\u30b0\u30ec\u30c3\u30af\u30b9\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u53cd\u5fdc\u306e\u52b9\u7387\u3068\u6bd4\u8f03\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u7406\u60f3\u7684\u306b\u306f\u3001\u30b7\u30f3\u30b0\u30eb\u53cd\u5fdc\u3068\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u306e\u9593\u306b\u306f\u307b\u3068\u3093\u3069\u5909\u5316\u304c\u306a\u3044\u306f\u305a\u3067\u3059\u3002\u3082\u3057\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u53cd\u5fdc\u52b9\u7387\u306e\u5909\u5316\u304c5%\u3092\u8d85\u3048\u3066\u3044\u308b\u304b\u3042\u308b\u3044\u306f\u597d\u307e\u3057\u3044\u7bc4\u56f2\u3067\u3042\u308b90%\uff5e110%\u306b\u53ce\u307e\u3089\u306a\u3044\u5834\u5408\u306b\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u307e\u305f\u306f\u30d7\u30ed\u30fc\u30d6\u306e\u6fc3\u5ea6\u307e\u305f\u306f\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30a2\u30c3\u30bb\u30a4\u4e2d\u306e\u4ed6\u306e\u6210\u5206\u306e\u6fc3\u5ea6\u3092\u6700\u9069\u5316\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u611f\u5ea6\u306e\u4f4e\u4e0b\u3092\u9632\u3050\u30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href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/real-time-pcr-handbook-form.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr54_Social_LAB\" target=\"_blank\" 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