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30b6\u30a4\u30f3\u306e\u6bb5\u968e\u306b\u304a\u3044\u3066\u7c21\u5358\u306a\u4e88\u9632\u7b56\u3092\u8b1b\u3058\u308b\u3053\u3068\u304c\u6700\u826f\u306e\u65b9\u6cd5\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u306e\u7121\u6599\u30c4\u30fc\u30eb\u304c\u3044\u304f\u3064\u304b\u5165\u624b\u53ef\u80fd\u3067\u3059\u3002\u305d\u306e\u3088\u3046\u306a\u30c4\u30fc\u30eb\u306e\u4e00\u4f8b\u304cAutoDimer\uff08\u7c73\u56fd\u56fd\u7acb\u6a19\u6e96\u6280\u8853\u7814\u7a76\u6240\u306eP.M. Vallone \u8457\uff09\u3067\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u306e\u914d\u5217\u3092\u89e3\u6790\u3057\u3001\u7406\u8ad6\u7684\u306b\u4e8c\u91cf\u4f53\u5f62\u6210\u3092\u8d77\u3053\u3057\u6613\u3044\u30d7\u30a2\u30ea\u30de\u30fc\u30da\u30a2\u306b\u8b66\u544a\u3092\u51fa\u3057\u307e\u3059\u3002<\/p>\n<p>\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u3092\u5229\u7528\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306b\u3088\u308a\u3001\u4e8c\u91cf\u4f53\u5f62\u6210\u306e\u53ef\u80fd\u6027\u3092\u4f4e\u6e1b\u3055\u305b\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u304c\u3001\u4e8c\u91cf\u4f53\u5f62\u6210\u3092\u5b9f\u9a13\u7684\u306b\u30e2\u30cb\u30bf\u30fc\u3059\u308b\u3053\u3068\u306f\u3084\u306f\u308a\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<h3>\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u6709\u7121\u306e\u6c7a\u5b9a<\/h3>\n<p>\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u6cd5\u306b\u3088\u308a\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u3092\u76ee\u8996\u3067\u78ba\u8a8d\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306f\u3001\u901a\u5e38100\u5869\u57fa\u5bfe\u3088\u308a\u3082\u77ed\u3044\u30b5\u30a4\u30ba\u306e\u5e83\u304c\u3063\u305f\u30d0\u30f3\u30c9\u3068\u3057\u3066\u78ba\u8a8d\u3067\u304d\u307e\u3059\uff08\u56f31\uff09\u3002PCR\u53cd\u5fdc\u306b\u304a\u3044\u3066\u306f\u3001\u30c0\u30a4\u30de\u30fc\u5f62\u6210\u3068\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u304a\u3088\u3073\u4f38\u9577\u304c\u7af6\u5408\u3057\u307e\u3059\u3002\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304c\u6e1b\u5c11\u3059\u308b\u306b\u3064\u308c\u3066\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u5897\u5e45\u3057\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u6cd5\u3092\u552f\u4e00\u306e\u691c\u8a3c\u65b9\u6cd5\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\u3053\u3068\u306e\u6b20\u70b9\u306f\u3001\u611f\u5ea6\u304c\u30ca\u30ce\u30b0\u30e9\u30e0\u9818\u57df\u3067\u3042\u308b\u305f\u3081\u7d50\u679c\u304c\u4e0d\u78ba\u5b9f\u306b\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3053\u3068\u3067\u3059\u3002\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u6cd5\u306e\u9577\u6240\u306f\u3001\u6e29\u5ea6\u89e3\u96e2\u66f2\u7dda\uff08\u878d\u89e3\u66f2\u7dda\uff09\u30c7\u30fc\u30bf\u304c\u5b58\u5728\u3059\u308b\u5834\u5408\u3001\u53cd\u5fdc\u7523\u7269\u306e\u30b5\u30a4\u30ba\u306e\u628a\u63e1\u304c\u5168\u4f53\u7684\u89e3\u91c8\u3059\u308b\u969b\u306b\u6709\u7528\u3068\u306a\u308b\u3053\u3068\u3067\u3059\u3002<\/p>\n<div id=\"attachment_2158\" style=\"width: 746px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig1.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2158\" class=\"size-full wp-image-2158\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig1.png\" alt=\"qpcr-basic49-fig1\" width=\"736\" height=\"288\" \/><\/a><p id=\"caption-attachment-2158\" class=\"wp-caption-text\">\u56f31 \u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u89e3\u6790\u306b\u3088\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u78ba\u8a8d\u3002DNA\u30b5\u30f3\u30d7\u30eb\u3092\u6bb5\u968e\u5e0c\u91c8\u3057\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30ea\u30f3\u30b0\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u7d42\u4e86\u5f8c\u306e\u53cd\u5fdc\u6db2\u3092\u7b49\u91cf\u305a\u3064\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306b\u6d41\u3057\u307e\u3057\u305f\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306f100bp\u672a\u6e80\u306e\u9818\u57df\u306e\u5e83\u304c\u3063\u305f\u30d0\u30f3\u30c9\u3068\u3057\u3066\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u91cf\u304c\u5c11\u306a\u304f\u306a\u308b\u306b\u3064\u308c\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u91cf\u304c\u5897\u3048\u308b\u3053\u3068\u3082\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u3002<\/p><\/div>\n<p>\u89e3\u96e2\u66f2\u7dda\u306f\u3001\u878d\u89e3\u66f2\u7dda\u3068\u3082\u79f0\u3055\u308c\u3001\u4e8c\u672c\u9396DNA\u7d50\u5408\u8272\u7d20\u3092\u4f7f\u7528\u3059\u308b\u53cd\u5fdc\u306e\u6a19\u6e96\u7684\u306a\u71b1\u7684\u5206\u6790\u624b\u6cd5\u3067\u3059\u3002\u975e\u5e38\u306b\u7279\u7570\u6027\u304c\u9ad8\u304f\u5897\u5e45\u3057\u305f\u5834\u5408\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30d7\u30ec\u30fc\u30c8\u4e0a\u306e\u5404\u30a6\u30a7\u30eb\u306b\u95a2\u3059\u308b\u878d\u89e3\u66f2\u7dda\u306b\u304a\u3044\u30661\u672c\u306e\u5e45\u306e\u72ed\u3044\u30d4\u30fc\u30af\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306f70\u00b0C\u7a0b\u5ea6\u306e\u9818\u57df\u3067\u306e\u878d\u89e3\u3092\u793a\u3059\u3001\u86cd\u5149\u5ea6\u306e\u4f4e\u3044\u5e45\u5e83\u306e\u300c\u6ce2\u300d\u3068\u3057\u3066\u73fe\u308c\u307e\u3059\u3002\u3053\u306e\u30d4\u30fc\u30af\u306e\u5f62\u3068Tm\u5024\u306e\u7279\u5fb4\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u30b5\u30a4\u30ba\u304c\u5c0f\u3055\u304f\u3001\u3057\u304b\u3082\u4e00\u5b9a\u3057\u3066\u3044\u306a\u3044\u3053\u3068\u306b\u8d77\u56e0\u3057\u3066\u3044\u307e\u3059\u3002\u30c7\u30fc\u30bf\u89e3\u6790\u306e\u9805\u3067\u793a\u3057\u305f\u3088\u3046\u306b\u3001\u878d\u89e3\u66f2\u7dda\u306b\u304a\u3044\u3066\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u5b58\u5728\u304c\u7591\u308f\u308c\u308b\u5834\u5408\u306b\u306f\u5e38\u306b\u3001NTC\u30a6\u30a7\u30eb\u3068\u306e\u6bd4\u8f03\u3092\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304c\u5b58\u5728\u3057\u306a\u3044\u5834\u5408\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u6700\u3082\u5f62\u6210\u3055\u308c\u3084\u3059\u3044\u3067\u3059\uff08\u56f32\uff09\u3002<\/p>\n<div id=\"attachment_2159\" style=\"width: 390px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig2.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2159\" class=\"size-full wp-image-2159\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig2.png\" alt=\"qpcr-basic49-fig2\" width=\"380\" height=\"869\" \/><\/a><p id=\"caption-attachment-2159\" class=\"wp-caption-text\">\u56f32 \uff08A\uff09\u7279\u7570\u7684\u5897\u5e45\u304a\u3088\u3073\uff08B\uff09\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u5f71\u97ff\u3092\u793a\u3059\u3001\u5897\u5e45\u30d7\u30ed\u30c3\u30c8\u304a\u3088\u3073\u878d\u89e3\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306f\uff08B\uff09\u306b\u304a\u3044\u3066\u5897\u5e45\u66f2\u7dda\u4e2d\u306eNTC\u306b\u3088\u308a\u751f\u6210\u3055\u308c\u305f\u30b7\u30b0\u30ca\u30eb\u304a\u3088\u3073\u878d\u89e3\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u4e2d\u306e\u8ffd\u52a0\u30d4\u30fc\u30af\u3068\u3057\u3066\u540c\u5b9a\u3055\u308c\u307e\u3059\u3002<\/p><\/div>\n<h3>\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u4f4e\u6e1b\u307e\u305f\u306f\u6392\u9664<\/h3>\n<p>\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u554f\u984c\u3068\u306a\u308b\u5834\u5408\u3001\u53cd\u5fdc\u306b\u304a\u3051\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306e\u751f\u6210\u3092\u4f4e\u6e1b\u307e\u305f\u306f\u6392\u9664\u3059\u308b\u305f\u3081\u306b\u3044\u304f\u3064\u304b\u306e\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<ol>\n<li>\u7b2c\u4e00\u306e\u65b9\u6cd5\u306f\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30eb\u6761\u4ef6\u306e\u6700\u9069\u5316\u3067\u3059\u304c\u3001\u3053\u308c\u306b\u306f\u4e3b\u3068\u3057\u3066\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u306e\u4e0a\u6607\u304c\u884c\u308f\u308c\u307e\u3059\u3002\u307b\u3068\u3093\u3069\u306e\u5834\u5408\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306f60\u00b0C\u3067\u52b9\u7387\u3088\u304f\u30a2\u30cb\u30fc\u30eb\u3059\u308b\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u3053\u308c\u306f2-Step \u306e\u30b5\u30a4\u30af\u30eb\uff0895\u00b0C\u306b\u304a\u3051\u308b\u5909\u6027\u6bb5\u968e\u304b\u3089\u76f4\u63a560\u00b0C\u306b\u304a\u3051\u308b\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u304a\u3088\u3073\u4f38\u9577\u30b9\u30c6\u30c3\u30d7\u306b\u9032\u307f\u307e\u3059\uff09\u306b\u3088\u308a\u5b89\u5b9a\u3057\u305f\u5897\u5e45\u304c\u53ef\u80fd\u3068\u306a\u308b\u305f\u3081\u3067\u3059\u3002<\/li>\n<li>\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u3092\u4e0b\u3052\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002\u3055\u3089\u306b\u3001\u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u6bd4\u3092\u5909\u5316\u3055\u305b\u308b\u3053\u3068\u304c\u6709\u7528\u3068\u306a\u308b\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u3002\u307b\u3068\u3093\u3069\u306e\u5834\u5408\u3001\u5404\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u6700\u7d42\u6fc3\u5ea6\u3068\u3057\u3066200nM\u304c\u7406\u60f3\u7684\u3067\u3059\u304c\u3001\u5fc5\u8981\u3067\u3042\u308c\u307060nM\u7a0b\u5ea6\u306b\u4f4e\u4e0b\u3055\u305b\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002<\/li>\n<li>\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u6fc3\u5ea6\u306f\u7d043mM\u304c\u6700\u9069\u3067\u3059\u3002\u3053\u308c\u4ee5\u4e0a\u306e\u6fc3\u5ea6\u3067\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u751f\u6210\u3057\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002<\/li>\n<li>\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u5f62\u6210\u3055\u308c\u308b\u304b\u3069\u3046\u304b\u8a55\u4fa1\u3055\u308c\u3066\u3044\u306a\u304b\u3063\u305f\u5834\u5408\u306b\u306f\u3001\u8a55\u4fa1\u3092\u884c\u3044\u3001\u5fc5\u8981\u3067\u3042\u308c\u3070\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306e\u5909\u66f4\u3092\u691c\u8a0e\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u901a\u5e38\u3001\u30db\u30c3\u30c8\u30b9\u30bf\u30fc\u30c8DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u4f7f\u7528\u304a\u3088\u3073\u3001\u53cd\u5fdc\u306e\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u3092\u6c37\u4e0a\u3067\u884c\u3046\u3053\u3068\u304c\u671b\u307e\u3057\u3044\u3067\u3059\u3002<\/li>\n<li>\u7406\u60f3\u7684\u306b\u306f\u3001\u540c\u4e00\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u95a2\u3057\u3066\u8907\u6570\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3092\u540c\u6642\u306b\u8a66\u9a13\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u5b9f\u969b\u3053\u306e\u65b9\u6cd5\u306b\u3088\u308a\u76f4\u3061\u306b\u4f7f\u7528\u53ef\u80fd\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u304c\u898b\u3064\u3051\u3089\u308c\u308c\u3070\u3001\u6700\u9069\u5316\u306b\u8cbb\u3084\u3059\u591a\u304f\u306e\u6642\u9593\u3092\u7bc0\u7d04\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3059\u3002<\/li>\n<\/ol>\n<p>1-Step 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id=\"attachment_2160\" style=\"width: 384px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig3.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2160\" class=\"size-full wp-image-2160\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig3.png\" alt=\"qpcr-basic49-fig3\" width=\"374\" height=\"845\" \/><\/a><p id=\"caption-attachment-2160\" class=\"wp-caption-text\">\u56f33 \u4fdd\u5b58\u304c\u4e0d\u9069\u5207\u3060\u3063\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u5f71\u97ff\u3092\u793a\u3059\u3002\u5897\u5e45\u30d7\u30ed\u30c3\u30c8\uff08\u878d\u89e3\u66f2\u7dda\u304a\u3088\u3073\u86cd\u5149\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306b\u3088\u308a\u793a\u3055\u308c\u3066\u3044\u307e\u3059\uff09\uff08A\uff09 vs. 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DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\uff09\u306a\u3069\u304c\u3042\u3052\u3089\u308c\u307e\u3059\u3002\u305d\u306e\u4ed6\u306e\u52b9\u7387\u306e\u4f4e\u4e0b\u3092\u5f15\u304d\u8d77\u3053\u3059\u8981\u56e0\u3068\u3057\u3066\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u9593\u306eTm\u306e\u5dee\u304c5\u00b0C\u4ee5\u4e0a\u3067\u3042\u308b\u3053\u3068\u3001\u304a\u3088\u3073\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30eb\u6761\u4ef6\u304c\u6700\u9069\u5316\u3055\u308c\u3066\u3044\u306a\u3044\u3053\u3068\u306a\u3069\u304c\u3042\u308a\u307e\u3059\u3002\u524d\u8ff0\u3057\u305f\u3088\u3046\u306b\u3001\u30c1\u30e5\u30fc\u30d6\u4e2d\u3067\u306e\u7af6\u5408\u306f\u53cd\u5fdc\u52b9\u7387\u304c\u4f4e\u304f\u306a\u308b\u539f\u56e0\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u52b9\u7387\u304c\u9ad8\u3044\u5834\u5408\u306b\u304a\u3044\u3066\u3082\u3042\u308b\u3044\u306f\u4f4e\u3044\u5834\u5408\u306b\u304a\u3044\u3066\u3082\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306e\u52b9\u7387\u3092\u5408\u81f4\u3055\u305b\u308b\u3053\u3068\u304c\u3001\u30c7\u30fc\u30bf\u306e\u7cbe\u5ea6\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306b\u306f\u975e\u5e38\u306b\u91cd\u8981\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001\u30bf\u30fc\u30b2\u30c3\u30c8A\u306b\u95a2\u3059\u308b\u52b9\u7387\u304c95%\u3067\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306b\u95a2\u3059\u308b\u52b9\u7387\u304c96%\u3067\u3042\u308b\u65b9\u304c\u3001\u30bf\u30fc\u30b2\u30c3\u30c8A\u306b\u95a2\u3059\u308b\u52b9\u7387\u304c99%\u3067\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u306b\u95a2\u3059\u308b\u52b9\u7387\u304c92%\u3067\u3042\u308b\u5834\u5408\u3088\u308a\u3082\u9069\u5207\u3067\u3059\u3002<\/p>\n<h3>\u6b6a\u3093\u3060\u52b9\u7387\u306e\u554f\u984c\u70b9<\/h3>\n<p>90%\uff5e110%\u306e\u7bc4\u56f2\u3092\u8d85\u3048\u308b\u52b9\u7387\u306f\u3001\u7d50\u679c\u306b\u4eba\u70ba\u7684\u306a\u6b6a\u307f\u3092\u751f\u3058\u3001\u8aa4\u3063\u305f\u7d50\u8ad6\u3078\u3068\u5c0e\u304f\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u3053\u308c\u306f\u4e3b\u306b\u6bd4\u8f03\u7528\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u7570\u306a\u308b\u52b9\u7387\u3092\u6709\u3059\u308b\u3053\u3068\u306b\u8d77\u56e0\u3057\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u963b\u5bb3\u304a\u3088\u3073\u52b9\u7387\u306e\u4f4e\u4e0b\u3082\u30a2\u30c3\u30bb\u30a4\u306e\u611f\u5ea6\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u3001\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u304c\u7e2e\u5c0f\u3057\u3001\u6c4e\u7528\u6027\u304c\u4f4e\u4e0b\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\uff08\u56f34\uff09\u3002<\/p>\n<div id=\"attachment_2163\" style=\"width: 553px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig4.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2163\" class=\"size-full wp-image-2163\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig4.png\" alt=\"qpcr-basic49-fig4\" width=\"543\" height=\"346\" \/><\/a><p id=\"caption-attachment-2163\" class=\"wp-caption-text\">\u56f34 \u53cd\u5fdc\u52b9\u7387\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u6bb5\u968e\u5e0c\u91c8\u3002\u65e9\u3044Ct \u5024\u3092\u793a\u3059\u5e0c\u91c8\u7269\u306b\u304a\u3044\u3066\u306fCt\u5024\u304c\u4e92\u3044\u306b\u63a5\u8fd1\u3057\u3066\u304a\u308a\u3001\u66f2\u7dda\u306f\u7570\u5e38\u306a\u5f62\u72b6\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304c\u3088\u308a\u5e0c\u91c8\u3055\u308c\u308b\u306b\u3064\u308c\u3066\u963b\u5bb3\u306e\u5f71\u97ff\u304c\u306a\u304f\u306a\u308a\u3001\u66f2\u7dda\u306f\u7279\u5fb4\u7684\u306a\u6307\u6570\u95a2\u6570\u7684\u5f62\u72b6\u76f8\u306b\u3088\u308a\u8fd1\u304f\u306a\u308a\u307e\u3059\u3002<\/p><\/div>\n<h3>\u52b9\u7387\u306e\u6b6a\u307f\u306e\u540c\u5b9a<\/h3>\n<p>\u524d\u8ff0\u3057\u305f\u3068\u304a\u308a\u3001\u7279\u5b9a\u306e\u30a2\u30c3\u30bb\u30a4\u304c\u975e\u52b9\u7387\u7684\u3067\u3042\u308b\u304b\u3069\u3046\u304b\u3092\u540c\u5b9a\u3059\u308b\u305f\u3081\u306e\u6700\u826f\u306e\u65b9\u6cd5\u306f\u3001\u672a\u77e5\u8a66\u6599\u306b\u304a\u3044\u3066\u671f\u5f85\u3055\u308c\u308b\u6fc3\u5ea6\u7bc4\u56f2\u306b\u304a\u3044\u3066\u5e0c\u91c8\u3055\u308c\u305f\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u6a19\u6e96\u66f2\u7dda\u3092\u4f5c\u6210\u3057\u3001\u305d\u306e\u7bc4\u56f2\u306b\u304a\u3051\u308b\u52b9\u7387\u3092\u89b3\u5bdf\u3059\u308b\u3053\u3068\u3067\u3059\u3002\u7d50\u679c\u306f\u307b\u307c100%\u3067\u3042\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<p>\u878d\u89e3\u66f2\u7dda\u307e\u305f\u306f\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u6cd5\u306b\u304a\u3044\u3066\u8907\u6570\u306e\u30d4\u30fc\u30af\u304c\u89b3\u5bdf\u3055\u308c\u308b\u5834\u5408\u306b\u306f\u3001\u53cd\u5fdc\u4e2d\u3067\u7af6\u5408\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3001\u305d\u306e\u7af6\u5408\u304c\u78ba\u5b9f\u306b\u53cd\u5fdc\u52b9\u7387\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u3066\u3044\u307e\u3059\u3002<\/p>\n<h3>\u52b9\u7387\u306e\u4f4e\u4e0b\u307e\u305f\u306f\u963b\u5bb3\u306e\u89e3\u6c7a<\/h3>\n<p>\u53cd\u5fdc\u304c\u963b\u5bb3\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u3001\u307e\u305f\u306f\u53cd\u5fdc\u306e\u52b9\u7387\u304c\u4f4e\u4e0b\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u5224\u660e\u3057\u305f\u5834\u5408\u306b\u3001\u52b9\u7387\u3092\u671b\u307e\u3057\u3044\u7bc4\u56f2\u306b\u623b\u3059\u3044\u304f\u3064\u304b\u306e\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<ol>\n<li>\u963b\u5bb3\u306b\u95a2\u3057\u3066\u306f\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u6fc3\u5ea6\u304c\u6700\u3082\u9ad8\u3044\u30a6\u30a7\u30eb\u3092\u9664\u3044\u3066\u691c\u91cf\u7dda\u3092\u89e3\u6790\u3057\u76f4\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3082\u3057\u52b9\u7387\u304c110%\u4ee5\u4e0b\u306b\u623b\u308c\u3070\u3001\u30a2\u30c3\u30bb\u30a4\u306b\u554f\u984c\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u4f46\u3057\u3001\u691c\u91cf\u7dda\u304b\u3089\u9664\u3044\u305f\u6fc3\u5ea6\u306f\u3059\u3079\u3066\u5b9f\u969b\u306e\u30a2\u30c3\u30bb\u30a4\u306b\u306f\u4f7f\u7528\u3057\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u3082\u3046\u4e00\u3064\u306e\u89e3\u6c7a\u6cd5\u3068\u3057\u3066\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u518d\u7cbe\u88fd\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u969b\u306b\u306f\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf\u7531\u6765\u306e\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u9664\u53bb\u3059\u308b\u305f\u3081\u306b\u4e7e\u71e5\u6642\u9593\u3092\u9577\u304f\u3059\u308b\u3053\u3068\u3001\u307e\u305f\u306f\u30b7\u30ea\u30ab\u30d9\u30fc\u30b9\u306e\u7cbe\u88fd\u7531\u6765\u306e\u30ab\u30aa\u30c8\u30ed\u30d4\u30c3\u30af\u5869\u3092\u9664\u53bb\u3059\u308b\u305f\u3081\u306b\u30ab\u30e9\u30e0\u4e0a\u3067\u306e\u7cbe\u88fd\u3092\u8ffd\u52a0\u3059\u308b\u3053\u3068\u3092\u5fd8\u308c\u306a\u3044\u3067\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u52b9\u7387\u306e\u4f4e\u4e0b\u306b\u5bfe\u3057\u3066\u306f\u30a2\u30c3\u30bb\u30a4\u306e\u6700\u9069\u5316\u306b\u3088\u3063\u3066\u89e3\u6c7a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u305d\u306e\u904e\u7a0b\u306f\u6bd4\u8f03\u7684\u7c21\u5358\u306a\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u304c\u3001\u5834\u5408\u306b\u3088\u3063\u3066\u306f\u30a2\u30c3\u30bb\u30a4\u306e\u8907\u96d1\u6027\u304c\u5897\u3059\u306b\u3064\u308c\u3066\u6700\u9069\u5316\u304c\u7169\u96d1\u306b\u306a\u308b\u53ef\u80fd\u6027\u3082\u3042\u308a\u307e\u3059\u3002<\/li>\n<li>\u5358\u4e00\u306e\u7523\u7269\u306e\u307f\u304c\u5897\u5e45\u3055\u308c\u308b\u5834\u5408\u306b\u306f\u3001\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u6fc3\u5ea6\u30926mM\u306b\u307e\u3067\u4e0a\u6607\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u308a\u52b9\u7387\u3092\u6539\u5584\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u304c\u3001\u7af6\u5408\u304c\u5b58\u5728\u3059\u308b\u5834\u5408\u306b\u306f\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u6fc3\u5ea6\u3092\u4f4e\u4e0b\u3055\u305b\u308b\u3053\u3068\u304c\u52b9\u679c\u7684\u3067\u3042\u308b\u53ef\u80fd\u6027\u3082\u3042\u308a\u307e\u3059\u3002<\/li>\n<li>\u4e3b\u3068\u3057\u3066\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u53cd\u5fdc\u306b\u304a\u3044\u3066\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306e\u6700\u9069\u5316\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u304c\u5fc5\u8981\u3068\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u3001\u7279\u5b9a\u306e\u30a2\u30c3\u30bb\u30a4\u306b\u95a2\u3059\u308b\u7406\u60f3\u7684\u306a\u6fc3\u5ea6\u306e\u7d44\u307f\u5408\u308f\u305b\u3092\u898b\u3064\u3051\u308b\u305f\u3081\u306b\u3001\u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u69d8\u3005\u306a\u6bd4\u3001\u305d\u3057\u3066\u6642\u306b\u306f\u7570\u306a\u308b\u30d7\u30ed\u30fc\u30d6\u6bd4\u3092\u8a66\u9a13\u3057\u307e\u3059\u3002\u7406\u60f3\u7684\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u306f100nM\uff5e600nM\u306e\u7bc4\u56f2\u3067\u3042\u308a\u3001\u7406\u60f3\u7684\u306a\u30d7\u30ed\u30fc\u30d6\u6fc3\u5ea6\u306f100nM\uff5e400nM\u306e\u9593\u3067\u3059\u3002<\/li>\n<li>\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30ea\u30f3\u30b0\u306e\u6761\u4ef6\uff08\u7279\u306b\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\uff09\u304c\u30d7\u30e9\u30a4\u30de\u30fc\u306eTm\u306b\u95a2\u3057\u3066\u597d\u9069\u3067\u3042\u308a\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u540c\u58eb\u304c\u540c\u69d8\u306aTm\u3092\u6709\u3059\u308b\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ol>\n<p>\u53cd\u5fdc\u306b\u95a2\u9023\u3057\u3066\u3044\u308b\u3088\u3046\u306b\u898b\u3089\u308c\u308b\u554f\u984c\u304c\u5b9f\u969b\u306f\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u95a2\u9023\u306e\u554f\u984c\u3067\u3042\u308b\u3068\u3044\u3046\u5834\u5408\u3082\u5b58\u5728\u3057\u307e\u3059\u3002\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306e\u8a2d\u5b9a\u3092\u691c\u8a3c\u3042\u308b\u3044\u306f\u6700\u9069\u5316\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u671f\u5f85\u306b\u6cbf\u3046\u7d50\u679c\u304c\u5f97\u3089\u308c\u308b\u30b1\u30fc\u30b9\u3082\u3057\u3070\u3057\u3070\u5b58\u5728\u3057\u307e\u3059\u3002<\/p>\n<h2>\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u89e3\u6790\u8a2d\u5b9a<\/h2>\n<p>\u5148\u306b\u8ff0\u3079\u305f\u3088\u3046\u306b\u3001\u89e3\u6790\u306b\u3088\u308a\u826f\u597d\u306a\u30a2\u30c3\u30bb\u30a4\u304c\u8868\u793a\u3055\u308c\u306a\u304f\u306a\u3063\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u5b58\u5728\u3059\u308b\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u3002\u30c7\u30fc\u30bf\u306e\u7cbe\u5ea6\u306b\u6700\u3082\u5927\u304d\u306a\u5f79\u5272\u3092\u679c\u305f\u3059\u89e3\u6790\u8a2d\u5b9a\u306f\u4ee5\u4e0b\u306e\u3068\u304a\u308a\u3067\u3059\u3002<\/p>\n<ol>\n<li>\u5897\u5e45\u66f2\u7dda\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u306e\u76f4\u7dda\u6027<\/li>\n<li>\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u30ec\u30f3\u30b8\u8a2d\u5b9a<\/li>\n<li>Threshold 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\u304c\u975e\u5e38\u306b\u65e9\u3044\u5834\u5408\u306b\u306f\u3001\u3059\u3067\u306b\u5e73\u3089\u3067\u306f\u306a\u3044\u90e8\u5206\u304c\u8aa4\u3063\u3066\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u306b\u542b\u307e\u308c\u3066\u3057\u307e\u3046\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u56f35\u306b\u306f\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u8a2d\u5b9a\u3092\u5909\u5316\u3055\u305b\u305f\u540c\u4e00\u306e\u30d7\u30ed\u30c3\u30c8\u3067\u3059\u3002\u56f35A\u306b\u306f\u3001\u30b5\u30a4\u30af\u30eb1\u304b\u3089\u30b5\u30a4\u30af\u30eb14\u306e\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u306b\u3088\u308b\u30d7\u30ed\u30c3\u30c8\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u86cd\u5149\u306f10\u30b5\u30a4\u30af\u30eb\u76ee\u304b\u3089\u691c\u51fa\u3055\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u3053\u306e\u8a2d\u5b9a\u306f\u5e45\u304c\u5e83\u3059\u304e\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u3068\u3057\u3066\u66f2\u7dda\u304c\u6c88\u307fCt \u304c\u9045\u304f\u306a\u308a\u307e\u3059\u3002\u56f35B\u306f\u3001\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u3092\u30b5\u30a4\u30af\u30eb2\u304b\u3089\u30b5\u30a4\u30af\u30eb8\u306e\u7bc4\u56f2\u306b\u30ea\u30bb\u30c3\u30c8\u3057\u305f\u7d50\u679c\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u66f2\u7dda\u304a\u3088\u3073Ct\u5024\u306f\u6b63\u78ba\u306a\u4f4d\u7f6e\u306b\u623b\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<div id=\"attachment_2164\" style=\"width: 1127px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig5.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2164\" class=\"size-full wp-image-2164\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig5.png\" alt=\"qpcr-basic49-fig5\" width=\"1117\" height=\"382\" \/><\/a><p id=\"caption-attachment-2164\" class=\"wp-caption-text\">\u56f35 \uff08A\uff09\u8a2d\u5b9a\u304c\u4e0d\u9069\u5207\u306a\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u304a\u3088\u3073\uff08B\uff09\u8a2d\u5b9a\u304c\u6b63\u3057\u3044\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u3092\u793a\u3059\u5897\u5e45\u30d7\u30ed\u30c3\u30c8<\/p><\/div>\n<p>\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u30ec\u30f3\u30b8\u306e\u8a2d\u5b9a\u306f\u3001\u3042\u307e\u308a\u983b\u7e41\u306b\u306f\u8003\u616e\u3055\u308c\u307e\u305b\u3093\u304c\u3001\u53cd\u5fdc\u306e\u52b9\u7387\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u56f36\u306b\u793a\u3059\u3088\u3046\u306b\u3001\u88c5\u7f6e\u306e\u521d\u671f\u8a2d\u5b9a\u306b\u3088\u308a\u307b\u3068\u3093\u3069\u306e\u30a2\u30c3\u30bb\u30a4\u3067\u9069\u5207\u306a\u7bc4\u56f2\u306b\u8a2d\u5b9a\u3055\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u624b\u52d5\u3067\u8abf\u6574\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u7d50\u679c\u3092\u3055\u3089\u306b\u6539\u5584\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306a\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<div id=\"attachment_2165\" style=\"width: 1149px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig6-1.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2165\" class=\"size-full wp-image-2165\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig6-1.png\" alt=\"qpcr-basic49-fig6\" width=\"1139\" height=\"484\" \/><\/a><p id=\"caption-attachment-2165\" class=\"wp-caption-text\">\u56f36 \u8a31\u5bb9\u53ef\u80fd\u306a\u53cd\u5fdc\u52b9\u7387\u3092\u7372\u5f97\u3059\u308b\u305f\u3081\u306e\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u8a2d\u5b9a\u6cd5\u306e\u6bd4\u8f03\u3002\uff08A\u304a\u3088\u3073D\uff09\u3053\u308c\u3089\u306e\u30d7\u30ed\u30c3\u30c8\u306b\u304a\u3051\u308b\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u306f\u624b\u52d5\u306b\u3088\u308a\u975e\u5e38\u306b\u5e83\u304f\u8a2d\u5b9a\u3055\u308c\u3066\u304a\u308a\u3001\u691c\u91cf\u7dda\u306e\u50be\u304d\u306f\u20132.81 \u3067\u3001\u597d\u307e\u3057\u3044\u7bc4\u56f2\u3067\u3042\u308b\u20133.58 \u304b\u3089\u20133.10\uff0890%\u304b\u3089110%\u306e\u52b9\u7387\u306b\u76f8\u5f53\uff09\u306e\u7bc4\u56f2\u304b\u3089\u5927\u304d\u304f\u5916\u308c\u3066\u3044\u307e\u3059\u3002\uff08C\u304a\u3088\u3073F\uff09\u3053\u3053\u306b\u306fA\u304a\u3088\u3073D\u3068\u540c\u4e00\u306e\u66f2\u7dda\u304c\u793a\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u306f\u88c5\u7f6e\u306b\u3088\u308a\u81ea\u52d5\u7684\u306b\u9078\u629e\u3055\u308c\u305f\u3082\u306e\u3067\u3059\u3002\u50be\u304d\u306f\u7406\u60f3\u7684\u306a\u7bc4\u56f2\u5185\u306b\u3042\u308a\u3001\u30a2\u30c3\u30bb\u30a4\u306f\u3053\u306e\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u306b\u308f\u305f\u3063\u3066\u691c\u8a3c\u3055\u308c\u3066\u3044\u307e\u3059\u3002\uff08B\u304a\u3088\u3073E\uff09\u3053\u308c\u3089\u306e\u30d7\u30ed\u30c3\u30c8\u306b\u304a\u3044\u3066\u306f\u624b\u52d5\u306b\u3088\u308b\u8abf\u6574\u304c\u884c\u308f\u308c\u3001\u3055\u3089\u306b4\uff5e5\u30b5\u30a4\u30af\u30eb\u304c\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u306b\u542b\u307e\u308c\u3066\u304a\u308a\u3001\u50be\u304d\u306f\u3055\u3089\u306b\u6539\u5584\u3055\u308c\u3001\u52b9\u7387\u304c\u307b\u307c100%\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/p><\/div>\n<p>Threshold Line \u306e\u8a2d\u5b9a\uff08\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3092\u8d85\u3048\u308b\u86cd\u5149\u30ec\u30d9\u30eb\u3067\u3001Ct \u6c7a\u5b9a\u306e\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u308b\u5024\uff09\u306f\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u3088\u308a\u81ea\u52d5\u7684\u306b\u8a2d\u5b9a\u3055\u308c\u308b\u3082\u3046\u4e00\u3064\u306e\u30d1\u30e9\u30e1\u30fc\u30bf\u3067\u3059\u304c\u3001\u3053\u308c\u3082\u624b\u52d5\u3067\u8abf\u6574\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\u540c\u4e00\u306e\u30e9\u30f3\u3067\u8907\u6570\u306e\u30ad\u30c3\u30c8\u307e\u305f\u306f\u30b1\u30df\u30b9\u30c8\u30ea\u3092\u8a55\u4fa1\u3059\u308b\u969b\u306b\u3001\u3057\u3070\u3057\u3070\u624b\u52d5\u3067\u306e\u8abf\u6574\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306f\u81ea\u52d5\u7684\u306b\u3001\u3088\u308a\u9ad8\u3044\u30d7\u30e9\u30c8\u30fc\u3092\u6709\u3059\u308b\u66f2\u7dda\uff08\u56f37\u306e\u9752\u8272\u306e\u30d7\u30ed\u30c3\u30c8\uff09\u306b\u7406\u60f3\u7684\u306aThreshold\u3092\u8a2d\u5b9a\u3057\u307e\u3059\u3002\u3057\u304b\u3057\u8d64\u8272\u306e\u30d7\u30ed\u30c3\u30c8\u306b\u95a2\u3059\u308b\u7406\u60f3\u7684\u306aThreshold\u306f\u3055\u3089\u306b\u4f4e\u3044\u5024\u3067\u3042\u308b\u305f\u3081\u3001\u3053\u306e\u7d50\u679c\u306f\u540c\u4e00\u306e\u30c7\u30fc\u30bf\u30bb\u30c3\u30c8\u4e2d\u306e\u8d64\u8272\u306e\u30d7\u30ed\u30c3\u30c8\u306eCt\u306b\u30d0\u30a4\u30a2\u30b9\u3092\u304b\u3051\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u5404\u30c7\u30fc\u30bf\u30bb\u30c3\u30c8\u3092\u5225\u3005\u306b\u89e3\u6790\u3057\u3001\u5404\u72b6\u6cc1\u306b\u5fdc\u3058\u3066\u7406\u60f3\u7684\u306aThreshold\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<div id=\"attachment_2166\" style=\"width: 558px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig7-1.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2166\" class=\"size-full wp-image-2166\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig7-1.png\" alt=\"qpcr-basic49-fig7\" width=\"548\" height=\"338\" \/><\/a><p id=\"caption-attachment-2166\" class=\"wp-caption-text\">\u56f37 \u5bfe\u6570\u30d7\u30ed\u30c3\u30c8\u306e\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8\u3002\u30d7\u30e9\u30c8\u30fc\u306e\u9ad8\u3055\u306e\u7570\u306a\u308b\u3001\u3059\u306a\u308f\u3061\u7570\u306a\u308b\u6307\u6570\u5bfe\u6570\u7684\u5897\u5e45\u671f\u3092\u6709\u3059\u308b2\u30bb\u30c3\u30c8\u306e\u5897\u5e45\u66f2\u7dda\u306e\u4f8b\u304c\u793a\u3055\u308c\u3066\u3044\u307e\u3059\u3002 \u5897\u5e45\u66f2\u7dda\u306b\u304a\u3044\u3066\u3001Ct \u6c7a\u5b9a\u306e\u305f\u3081\u306b\u6700\u3082\u6b63\u78ba\u306a\u90e8\u5206\u306f\u5bfe\u6570\u30d7\u30ed\u30c3\u30c8\u306b\u304a\u3051\u308b\u6307\u6570\u95a2\u6570\u7684\u5897\u5e45\u671f\u306e\u3061\u3087\u3046\u3069\u771f\u4e2d\u306b\u5f53\u305f\u308a\u307e\u3059\u3002\u7406\u60f3\u7684\u306a\u95be\u5024\u306e\u8a2d\u5b9a\u306f\u5404\u30c7\u30fc\u30bf\u30bb\u30c3\u30c8\u306b\u3088\u3063\u3066\u7570\u306a\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<\/p><\/div>\n<p>\u307e\u305f\u3001\u521d\u671f\u8a2d\u5b9a\u3067\u591a\u304f\u306e\u5834\u5408\u6975\u3081\u3066\u9069\u5207\u306a\u4f4d\u7f6e\u306b\u8a2d\u5b9a\u3055\u308c\u307e\u3059\u304c\u3001\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u3055\u3089\u306b\u7cbe\u5ea6\u3092\u5411\u4e0a\u3055\u305b\u308b\u305f\u3081\u306b\u3001Threshold\u3092\u6307\u6570\u95a2\u6570\u7684\u5897\u5e45\u671f\u306e\u771f\u4e2d\u306b\u624b\u52d5\u3067\u8a2d\u5b9a\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\u691c\u91cf\u7dda\u306e\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u306e\u691c\u8a3c\u306b\u3088\u308a\u3001\u30a2\u30c3\u30bb\u30a4\u6bce\u306b\u8a31\u5bb9\u53ef\u80fd\u306a\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u6fc3\u5ea6\u304c\u6c7a\u5b9a\u3055\u308c\u307e\u3059\u3002\u691c\u91cf\u7dda\u306e\u6700\u9ad8\u307e\u305f\u306f\u6700\u4f4e\u6fc3\u5ea6\u304c\u5b9f\u969b\u306e\u89e3\u6790\u306b\u304a\u3044\u3066\u4f7f\u7528\u3055\u308c\u306a\u3044\u5834\u5408\u306b\u9650\u308a\u3001\u53cd\u5fdc\u52b9\u7387\u3092\u5411\u4e0a\u3055\u305b\u308b\u305f\u3081\u306b\u3053\u308c\u3089\u306e\u6fc3\u5ea6\u3092\u6392\u9664\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\uff08\u56f38\uff09\u3002<\/p>\n<p>\u4e00\u822c\u7684\u306b\u3001\u52b9\u7387\u304c\u4f4e\u4e0b\u3059\u308b\u5834\u5408\u306b\u306f\u3044\u3064\u3067\u3082\u4e21\u7aef\u306e\u30c7\u30fc\u30bf\u30dd\u30a4\u30f3\u30c8\u3092\u6392\u9664\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308b\u305f\u3081\u3001\u975e\u5e38\u306b\u9ad8\u3044\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304b\u3089\u975e\u5e38\u306b\u4f4e\u3044\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u307e\u3067\u691c\u51fa\u9650\u754c\u3092\u5e83\u3052\u3066\u304a\u304f\u306e\u3082\u3088\u3044\u3067\u3057\u3087\u3046\u3002<\/p>\n<div id=\"attachment_2167\" style=\"width: 558px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig8-1.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2167\" class=\"size-full wp-image-2167\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig8-1.png\" alt=\"qpcr-basic49-fig8\" width=\"548\" height=\"326\" \/><\/a><p id=\"caption-attachment-2167\" class=\"wp-caption-text\">\u56f38 \u7570\u5e38\u5024\u3092\u6392\u9664\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u9054\u6210\u3055\u308c\u305f\u691c\u91cf\u7dda\u306e\u50be\u304d\u306e\u6539\u5584\u30025\u6841\u306b\u308f\u305f\u308b\u6bb5\u968e\u5e0c\u91c8\u306b\u95a2\u3057\u3066\u5897\u5e45\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u3053\u306e\u7bc4\u56f2\u306b\u304a\u3044\u3066\u3001\u50be\u304d\u306f\u20132.9\u3067\u3042\u308a\u3001\u597d\u307e\u3057\u3044\u52b9\u7387\u306e\u7bc4\u56f2\u304b\u3089\u5916\u308c\u3066\u3044\u307e\u3059\u3002\u4e0b\u6bb5\u306e\u66f2\u7dda\u3067\u306f\u3001\u691c\u91cf\u7dda\u304b\u3089\u5e0c\u91c8\u5ea6\u306e\u6700\u3082\u9ad8\u3044\u70b9\u3092\u6392\u9664\u3057\u3066\u52b9\u7387\u3092\u518d\u89e3\u6790\u3057\u307e\u3057\u305f\u3002\u50be\u304d\u306f\u20133.1\u306b\u6539\u5584\u3057\u3001\u30a2\u30c3\u30bb\u30a4\u3092\u691c\u8a3c\u3059\u308b\u305f\u3081\u306b\u5341\u5206\u306a\u52b9\u7387\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p><\/div>\n<p>ROX\u304a\u3088\u3073\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306a\u3069\u306e\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306e\u4f7f\u7528\u306f\u3001\u88c5\u7f6e\u304a\u3088\u3073\u30e6\u30fc\u30b6\u30fc\u306b\u8d77\u56e0\u3059\u308b\u30a8\u30e9\u30fc\u306e\u5f71\u97ff\u3092\u6392\u9664\u3059\u308b\u305f\u3081\u306e\u5f37\u529b\u306a\u65b9\u6cd5\u3067\u3059\u3002\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306e\u4f7f\u7528\u306f\u975e\u5e38\u306b\u4e00\u822c\u7684\u306b\u306a\u3063\u3066\u3044\u308b\u305f\u3081\u3001\u3053\u306e\u300c\u821e\u53f0\u88cf\u306e\u300d\u56e0\u5b50\u306f\u3001\u591a\u304f\u306e\u5834\u5408\u5fd8\u308c\u3089\u308c\u305f\u308a\u3001\u53cd\u5fdc\u306b\u8ca0\u306e\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u3053\u3068\u306f\u306a\u3044\u3068\u307f\u306a\u3055\u308c\u305f\u308a\u3057\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u88c5\u7f6e\u3068\u8272\u7d20\u81ea\u4f53\u306e\u95a2\u4fc2\u3092\u7406\u89e3\u3057\u3066\u304a\u304f\u3053\u3068\u306f\u91cd\u8981\u3067\u3059\u3002<\/p>\n<p>\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u3092\u4f7f\u7528\u3059\u308b\u88c5\u7f6e\u306b\u304a\u3044\u3066\u306f\u3001\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u3088\u308a\u4e0d\u6d3b\u6027\u8272\u7d20\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u767a\u3059\u308b\u86cd\u5149\u304b\u3089\u5dee\u3057\u5f15\u304b\u308c\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u4f8b\u3048\u3070ROX\u306e\u30ec\u30d9\u30eb\u304c\u9ad8\u3059\u304e\u308b\u5834\u5408\u306b\u306f\u3001\u5f97\u3089\u308c\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u30b7\u30b0\u30ca\u30eb\u304c\u975e\u5e38\u306b\u4f4e\u304f\u306a\u308a\u3001\u6ce2\u72b6\u3067\u4e00\u8cab\u6027\u306e\u306a\u3044\u5897\u5e45\u30d7\u30ed\u30c3\u30c8\u3092\u4e0e\u3048\u307e\u3059\uff08\u56f39A\uff09\u3002<\/p>\n<p>\u4e00\u898b\u3057\u305f\u3068\u3053\u308d\u3001\u53cd\u5fdc\u304c\u5931\u6557\u3057\u3001\u591a\u304f\u306e\u6700\u9069\u5316\u304c\u5fc5\u8981\u3067\u3042\u308b\u3088\u3046\u306b\u601d\u308f\u308c\u307e\u3059\u304c\u3001\u3044\u3063\u305f\u3044\u3069\u3053\u304b\u3089\u59cb\u3081\u308c\u3070\u3088\u3044\u306e\u3067\u3057\u3087\u3046\u304b\uff1f \u3057\u304b\u3057\u3001\u30ce\u30fc\u30de\u30e9\u30a4\u30b6\u30fc\u3068\u3057\u3066\u306eROX\u30c1\u30e3\u30cd\u30eb\u306e\u30b9\u30a4\u30c3\u30c1\u3092\u5207\u308a\u3001\u30c7\u30fc\u30bf\u3092\u518d\u89e3\u6790\u3059\u308b\u3068\u3001\u5b9f\u969b\u306e\u3068\u3053\u308d\u30c7\u30fc\u30bf\u306f\u9069\u5207\u3067\u3042\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\uff08\u56f39B\uff09\u3002\u3064\u307e\u308a\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306e\u6a19\u6e96\u5316\u306e\u554f\u984c\u3067\u3042\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u3082\u3057ROX\u3092\u542b\u307e\u306a\u3044\u8a66\u85ac\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408\u306f\u3001\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306e\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u30aa\u30d7\u30b7\u30e7\u30f3\u3092\u5fc5\u305a\u201cNone\u201d\u306b\u8a2d\u5b9a\u3057\u3066\u89e3\u6790\u3057\u3066\u3044\u305f\u3060\u304f\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<div id=\"attachment_2168\" style=\"width: 1133px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig9-1.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-2168\" class=\"size-full wp-image-2168\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic49-fig9-1.png\" alt=\"qpcr-basic49-fig9\" width=\"1123\" height=\"352\" \/><\/a><p id=\"caption-attachment-2168\" class=\"wp-caption-text\">\u56f39 \u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306b\u95a2\u3059\u308b\u88dc\u6b63\u3002\uff08A\uff09 ROX\u306a\u3069\u306e\u30d1\u30c3\u30b7\u30d6\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306e\u30ec\u30d9\u30eb\u304c\u9ad8\u3044\u5834\u5408\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u4f4e\u304f\u306a\u308a\u307e\u3059\u3002\uff08B\uff09ROX\u306e\u30b7\u30b0\u30ca\u30eb\u3092\u89e3\u6790\u304b\u3089\u9664\u53bb\u3059\u308b\u3068\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30b7\u30b0\u30ca\u30eb\u306f\u671f\u5f85\u3055\u308c\u308b\u7bc4\u56f2\u306b\u53ce\u307e\u308a\u307e\u3059\u3002<\/p><\/div>\n<p>\u524d\u8ff0\u3057\u305f\u3068\u304a\u308a\u3001\u88c5\u7f6e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306e\u8a2d\u5b9a\u306f\u307b\u3068\u3093\u3069\u306e\u5834\u5408\u9069\u5207\u306b\u884c\u308f\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u3053\u308c\u3089\u306e\u8a2d\u5b9a\u3092\u691c\u8a3c\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u30c7\u30fc\u30bf\u306e\u7cbe\u5ea6\u306e\u4fe1\u983c\u5ea6\u3092\u3055\u3089\u306b\u5411\u4e0a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u3088\u3063\u3066\u9078\u629e\u3055\u308c\u305f\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u304c\u5897\u5e45\u66f2\u7dda\u306e\u5e73\u3089\u306a\u7bc4\u56f2\u306e\u307f\u306b\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3001\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u306e\u30ec\u30f3\u30b8\u3092\u5897\u52a0\u307e\u305f\u306f\u6e1b\u5c11\u3055\u305b\u3066\u304f\u3060\u3055\u3044\u3002\u5897\u5e45\u66f2\u7dda\u3092\u5bfe\u6570\u30d7\u30ed\u30c3\u30c8\u3067\u89b3\u5bdf\u3057\u3001Threshold \u304c\u6307\u6570\u95a2\u6570\u7684\u5897\u5e45\u671f\u306e\u771f\u4e2d\u4ed8\u8fd1\u306b\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002y\u8ef8\u3092\u86cd\u5149\u30d7\u30e9\u30c8\u30fc\u5f37\u5ea6\u306b\u5bfe\u3057\u3066\u9069\u5207\u306b\u30b9\u30b1\u30fc\u30eb\u5316\u3057\u3001\u7570\u5e38\u5024\u3092\u6a19\u6e96\u66f2\u7dda\u304b\u3089\u6392\u9664\u3057\u3066\u52b9\u7387\u304a\u3088\u3073R2 \u5024\u3092\u6539\u5584\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u306b\u7559\u610f\u3057\u3066\u304f\u3060\u3055\u3044\uff08\u3053\u308c\u3089\u306e\u5e0c\u91c8\u304c\u300c\u672a\u77e5\u300d\u8a66\u6599\u306e\u8a55\u4fa1\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u306a\u3044\u5834\u5408\u306b\u9650\u308a\u307e\u3059\uff09\u3002<\/p>\n<p>\u6700\u5f8c\u306b\u3001\u6bd4\u8f03\u3092\u884c\u3046\u30a2\u30c3\u30bb\u30a4\u9593\u3067\u306f\u3001\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u304a\u3088\u3073Threshold \u306e\u8a2d\u5b9a\u304c\u540c\u4e00\u3067\u3042\u308b\u3053\u3068\u304c\u5fc5\u8981\u306a\u70b9\u306b\u7559\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30a2\u30c3\u30bb\u30a4\u306e\u691c\u8a3c\u304a\u3088\u3073\u4f7f\u7528\u306b\u304a\u3044\u3066\u907f\u3051\u308b\u3053\u3068\u306e\u3067\u304d\u306a\u3044\u554f\u984c\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u4ee5\u4e0b\u306e\u3088\u3046\u306b\u91cd\u8981\u70b9\u3092\u6574\u7406\u3057\u3066\u7406\u89e3\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u6bd4\u8f03\u7684\u7c21\u5358\u306a\u30d7\u30ed\u30bb\u30b9\u3068\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<ul>\n<li>\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u30b7\u30b0\u30ca\u30eb\u307e\u305f\u306f\u53cd\u5fdc\u52b9\u7387\u306e\u4f4e\u4e0b\u306b\u5f71\u97ff\u3057\u3066\u3044\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u30d7\u30e9\u30a4\u30de\u30fc\u304a\u3088\u3073\u30d7\u30ed\u30fc\u30d6\u306e\u5b89\u5b9a\u6027\u3092\u7dad\u6301\u3059\u308b\u306e\u306b\u5fc5\u8981\u306a\u5bfe\u7b56\u3092\u8b1b\u3058\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u53cd\u5fdc\u8a55\u4fa1\u306e\u6700\u7d42\u6bb5\u968e\u3068\u3057\u3066\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>90%\u4ee5\u4e0b\u306e\u52b9\u7387\u306f110%\u3092\u8d85\u3048\u308b\u52b9\u7387\u3068\u306f\u975e\u5e38\u306b\u7570\u306a\u308b\u3082\u306e\u3067\u3042\u308b\u3053\u3068\u3092\u7406\u89e3\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u88c5\u7f6e\u306e\u89e3\u6790\u8a2d\u5b9a\u3092\u691c\u8a3c\u3057\u8abf\u6574\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<h3>\u5897\u5e45\u3057\u306a\u3044<\/h3>\n<p>\u30a2\u30c3\u30bb\u30a4\u306b\u3088\u3063\u3066\u306f\u3001\u5168\u304f\u5897\u5e45\u3057\u306a\u3044\u554f\u984c\u304c\u767a\u751f\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u306b\u3054\u8aac\u660e\u3057\u305f\u3059\u3079\u3066\u306e\u30b9\u30c6\u30c3\u30d7\u3092\u884c\u3063\u3066\u3082\u5897\u5e45\u3057\u306a\u3044\u5834\u5408\u3001\u767a\u73fe\u91cf\u304c\u5c11\u306a\u3044\u3001\u9006\u8ee2\u5199\u306e\u554f\u984c\u3001\u30a2\u30c3\u30bb\u30a4\u30c7\u30b6\u30a4\u30f3\u306e\u554f\u984c\u306a\u3069\u3001\u305d\u306e\u4ed6\u306e\u8981\u56e0\u3067\u5897\u5e45\u3057\u3066\u3044\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h4>\u767a\u73fe\u91cf\u304c\u5c11\u306a\u3044<\/h4>\n<p>\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3092\u884c\u3046\u969b\u306b\u306f1-100 ng \u306ecDNA\u3092\u7528\u3044\u308b\u306e\u304c\u4e00\u822c\u7684\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u8abf\u3079\u305f\u3044\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u304c\u4f4e\u3044\u5834\u5408\u306b\u306f\u4f7f\u7528\u91cf\u3092\u3082\u3063\u3068\u5897\u3084\u3059\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u3001\u4f7f\u7528\u3067\u304d\u308b\u4e0a\u9650\u91cf\u307e\u3067\u306e\u7bc4\u56f2\u5185\u3067\u3001cDNA\u4f7f\u7528\u91cf\u3092\u632f\u3063\u3066\u53cd\u5fdc\u3057\u307e\u3059\u3002\u30a2\u30c3\u30bb\u30a4\u304c\u304d\u3061\u3093\u3068\u6a5f\u80fd\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u305f\u3081\u306b\u3001\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u3082\u540c\u6642\u306b\u53cd\u5fdc\u3059\u308b\u306e\u304c\u7406\u60f3\u7684\u3067\u3059\u3002\u3069\u306e\u7a0b\u5ea6\u306e\u767a\u73fe\u91cf\u3067\u3042\u308b\u304b\u4e0d\u660e\u306a\u5834\u5408\u306b\u306f\u3001\u6587\u732e\u3092\u3054\u53c2\u7167\u3044\u305f\u3060\u304f\u304b\u3001NCBI Unigene\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306eEST\u306e\u767a\u73fe\u30c7\u30fc\u30bf\u304b\u3089\u7570\u306a\u308b\u7d44\u7e54\u306e\u767a\u73fe\u91cf\u6bd4\u3092\u3054\u53c2\u7167\u3044\u305f\u3060\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<h4>\u9006\u8ee2\u5199\u306b\u95a2\u3059\u308b\u554f\u984c\u304c\u3042\u308b<\/h4>\n<p>\u4f4e\u767a\u73fe\u306b\u95a2\u9023\u3057\u307e\u3059\u304c\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u76ee\u7684\u907a\u4f1d\u5b50\u304c\u4f4e\u767a\u73fe\u306e\u5834\u5408\u306b\u306f\u3001\u30a2\u30c3\u30bb\u30a4\u306e\u611f\u5ea6\u3092\u4e0a\u3052\u308b\u5fc5\u8981\u304c\u3042\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002qPCR\u53cd\u5fdc\u306b\u5bfe\u3057\u3066cDNA\u306e\u4f7f\u7528\u91cf\u304c\u591a\u3059\u304e\u306a\u3044\u304b\u3069\u3046\u304b\u3092\u30c1\u30a7\u30c3\u30af\u3057\u307e\u3059\uff08\u901a\u5e38\u6700\u5927\u306e\u4f7f\u7528\u91cf\u306fPCR\u53cd\u5fdc\u6db2\u91cf\u306b\u5bfe\u3057\u306620% v\/v\uff09\u3002\u4f7f\u7528\u3059\u308bcDNA\u304c\u591a\u3059\u304e\u308b\u3068\u3001\u53cd\u5fdc\u6db2\u306b\u3088\u308a\u591a\u304f\u306e\u963b\u5bb3\u7269\u8cea\u304c\u6df7\u5165\u3057\u3001\u53cd\u5fdc\u306e\u52b9\u7387\u3092\u4f4e\u4e0b\u3055\u305b\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u4f7f\u7528\u3059\u308b\u9006\u8ee2\u5199\u9175\u7d20\u3068\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30bf\u30a4\u30d7\u3092\u30c1\u30a7\u30c3\u30af\u3059\u308b\u3053\u3068\u3082\u6709\u52b9\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u4e00\u822c\u306b\u9006\u8ee2\u5199\u30d7\u30e9\u30a4\u30de\u30fc\u306foligo\uff08dT\uff09\u3088\u308a\u3082\u30e9\u30f3\u30c0\u30e0\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u4f7f\u7528\u3059\u308b\u65b9\u304c\u53ce\u91cf\u306f\u591a\u304f\u306a\u308a\u307e\u3059\u3002\u307e\u305f\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/reverse-transcription\/reverse-transcriptase-enzymes.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr55_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Invitrogen\u2122 SuperScript\u2122 III Reverse Transcriptase<\/a> 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UTR 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