{"id":8320,"date":"2022-01-28T17:25:15","date_gmt":"2022-01-28T08:25:15","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=8320"},"modified":"2022-02-15T09:17:13","modified_gmt":"2022-02-15T00:17:13","slug":"hids_quantification_kit_ce_gsd_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/hids_quantification_kit_ce_gsd_ts_1\/","title":{"rendered":"\u9069\u5207\u306a\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u306e\u7d50\u679c\u3092\u5f97\u308b\u305f\u3081\u306e\u65b0\u3057\u3044\u5b9a\u91cf\u30ad\u30c3\u30c8"},"content":{"rendered":"

DNA\u578b\u3092\u5f97\u308b\u524d\u306bDNA\u30b5\u30f3\u30d7\u30eb\u306e\u8cea\u3092\u8a55\u4fa1\u3059\u308b\u65b9\u6cd5\u304c\u3042\u3063\u305f\u3068\u3057\u305f\u3089\u3068\u3066\u3082\u6709\u7528\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\uff1f\u72af\u7f6a\u73fe\u5834\u3088\u308a\u56de\u53ce\u3055\u308c\u308b\u30b5\u30f3\u30d7\u30eb\u306b\u306f\u3001\u8840\u75d5\u3001\u553e\u6db2\u3001\u76ae\u819a\u306e\u7d30\u80de\u306a\u3069\u3055\u307e\u3056\u307e\u306a\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u3002\u5f97\u3089\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u306eDNA\u6fc3\u5ea6\u3092\u6b63\u78ba\u306b\u898b\u7a4d\u3082\u308b\u3053\u3068\u304c\u3067\u304d\u308c\u3070\u3001\u3053\u306eDNA\u6fc3\u5ea6\u3092\u57fa\u306b\u4fe1\u983c\u6027\u306e\u9ad8\u3044DNA\u578b\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u4e00\u65b9\u3067DNA\u6fc3\u5ea6\u4ee5\u5916\u306e\u5f71\u97ff\u306b\u3088\u308a\u3001\u9069\u5207\u306aDNA\u578b\u304c\u5f97\u3089\u308c\u306a\u304b\u3063\u305f\u3053\u3068\u3082\u3042\u308b\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u3053\u306e\u8a18\u4e8b\u3067\u306f\u3001\u5b9a\u91cf\u306e\u904e\u7a0b\u3067\u6fc3\u5ea6\u4ee5\u5916\u306b\u3082DNA\u306e\u8cea\u306e\u60c5\u5831\u304c\u5f97\u3089\u308c\u308bApplied Biosystems\u2122 Quantifiler Trio Quantification Kit\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\u3053\u306e\u30ad\u30c3\u30c8\u306fDNA\u6fc3\u5ea6\u306b\u52a0\u3048\u3066\u3001DNA\u306e\u5206\u89e3\u3001\u30b5\u30f3\u30d7\u30eb\u5185\u306b\u5b58\u5728\u3059\u308b\u963b\u5bb3\u7269\u8cea\u3001\u7537\u6027\u907a\u4f1d\u5b50\u306e\u5b58\u5728\u3092\u8a55\u4fa1\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01
\n\u30fb\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u3092\u5b9f\u65bd\u3057\u3066\u3044\u308b
\n\u30fb\u5b89\u5b9a\u3057\u305fDNA\u578b\u3092\u5f97\u305f\u3044<\/p>\n

DNA\u30b5\u30f3\u30d7\u30eb\u306e\u5206\u89e3\u304cDNA\u578b\u30c7\u30fc\u30bf\u306b\u4e0e\u3048\u308b\u5f71\u97ff<\/h2>\n

DNA\u306e\u5206\u89e3\u306f\u5149\u3001\u71b1\u3084\u6e7f\u5ea6\u306a\u3069\u306e\u30b5\u30f3\u30d7\u30eb\u304c\u5b58\u5728\u3059\u308b\u74b0\u5883\u306e\u5f71\u97ff\u306b\u3088\u308a\u751f\u3058\u307e\u3059\u3002\u4ee5\u4e0b\u306e\u56f3\u306fDNA\u304c\u5206\u89e3\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3067\u898b\u3089\u308c\u308b\u30d1\u30bf\u30fc\u30f3\u3067\u3059\u3002\u5927\u304d\u3044\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u307b\u3069DNA\u5206\u89e3\u306e\u5f71\u97ff\u3092\u53d7\u3051\u3066\u5897\u5e45\u3067\u304d\u306a\u304f\u306a\u308b\u305f\u3081\u3053\u306e\u3088\u3046\u306a\u30d1\u30bf\u30fc\u30f3\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

\"\"<\/p>\n

Quantifiler Trio Quantification Kit\u3092\u7528\u3044\u305f\u8a55\u4fa1\u3067\u306f\u3001\u5206\u89e3\u3092\u8a55\u4fa1\u3059\u308b\u6307\u6a19\u306b\u5206\u89e3\u6307\u6570Degradation Index\uff08DI\uff09\u3092\u7b97\u51fa\u3057\u3066\u3044\u307e\u3059\u3002\u5206\u89e3\u6307\u6570\u3068\u306f\u4ee5\u4e0b\u306e\u5f0f\u306e\u69d8\u306bQuantifiler Trio kit\u3067\u5b9a\u91cf\u3055\u308c\u308b\u5c0f\u3055\u3044\u5e38\u67d3\u8272\u4f53\u4e0a\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u5927\u304d\u3044\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u6fc3\u5ea6\u6bd4\u304b\u3089\u81ea\u52d5\u3067\u7b97\u51fa\u3055\u308c\u307e\u3059\u3002
\n\"\"
\n\u3053\u306e\u6bd4\u304c\u22641\u3067\u3042\u308c\u3070\u5206\u89e3\u3055\u308c\u3066\u3044\u306a\u3044DNA\u3068\u3057\u3066\u8a55\u4fa1\u3067\u304d\u307e\u3059\u3002\u4e00\u65b91\u3092\u8d85\u3048\u305f\u5834\u5408\u306fDNA\u304c\u5206\u89e3\u3057\u3066\u3044\u308b\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u3066\u3001\u6b21\u306eSTR\u5897\u5e45\u306b\u4f7f\u7528\u3059\u308bDNA\u91cf\u3092\u6c7a\u5b9a\u3059\u308b\u5224\u65ad\u6750\u6599\u3068\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

DNA\u30b5\u30f3\u30d7\u30eb\u306e\u963b\u5bb3\u7269\u8cea\u304cDNA\u578b\u30c7\u30fc\u30bf\u306b\u4e0e\u3048\u308b\u5f71\u97ff<\/h2>\n

\u6b21\u306bDNA\u5897\u5e45\u6642\u306e\u963b\u5bb3\u7269\u8cea\u306b\u3064\u3044\u3066\u8003\u3048\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u30b5\u30f3\u30d7\u30eb\u62bd\u51fa\u6642\u306b\u6df7\u5165\u3057\u3066\u3057\u307e\u3063\u305f\u963b\u5bb3\u7269\u8cea\u306f\u305d\u306e\u5f8c\u306eDNA\u578b\u306b\u3082\u5f71\u97ff\u3092\u4e0e\u3048\u307e\u3059\u3002\u4ee5\u4e0b\u306e\u56f3\u306e\u3088\u3046\u306b\u5206\u89e3DNA\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u3066\u4f7f\u7528\u3057\u305f\u3068\u304d\u3068\u540c\u69d8\u306b\u5927\u304d\u3044\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306b\u5f71\u97ff\u304c\u307f\u3089\u308c\u307e\u3059\u3002
\n\"\"
\n\u540c\u3058\u3088\u3046\u306a\u30c7\u30fc\u30bf\u304c\u5f97\u3089\u308c\u305f\u304b\u3089\u3068\u3044\u3063\u3066\u5206\u89e3\u3068\u963b\u5bb3\u3092\u540c\u69d8\u306e\u3082\u306e\u3068\u8003\u3048\u3066\u306f\u3044\u3051\u307e\u305b\u3093\u3002\u305f\u3068\u3048\u3070\u5206\u89e3\u304c\u751f\u3058\u305f\u30b5\u30f3\u30d7\u30eb\u306fDNA\u91cf\u3092\u5897\u3084\u3059\u3053\u3068\u306b\u3088\u308a\u30d4\u30fc\u30af\u306e\u30b7\u30b0\u30ca\u30eb\u306e\u5897\u52a0\u304c\u307f\u3089\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u963b\u5bb3\u304c\u307f\u3089\u308c\u308b\u30b5\u30f3\u30d7\u30eb\u306fDNA\u91cf\u3092\u5897\u3084\u3059\u3053\u3068\u306b\u3088\u308a\u3001\u9006\u306b\u30b5\u30f3\u30d7\u30eb\u5185\u306e\u963b\u5bb3\u7269\u8cea\u306e\u6301\u3061\u8fbc\u307f\u304c\u591a\u304f\u306a\u308b\u305f\u3081\u5f71\u97ff\u304c\u5927\u304d\u304f\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002Quantifiler Trio kit\u306f\u30a4\u30f3\u30bf\u30fc\u30ca\u30ebPCR\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u3066\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u3068TaqMan\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u3092\u30ad\u30c3\u30c8\u6eb6\u6db2\u5185\u306b\u52a0\u3048\u3066\u30b5\u30f3\u30d7\u30eb\u5185\u306e\u963b\u5bb3\u3092\u8a55\u4fa1\u3057\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306b\u963b\u5bb3\u7269\u8cea\u304c\u542b\u307e\u308c\u308b\u5834\u5408\u306f\u30a4\u30f3\u30bf\u30fc\u30ca\u30ebPCR\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u5897\u5e45\u306b\u5f71\u97ff\u304c\u751f\u3058\u3001Ct\u5024\u304c\u5927\u304d\u304f\u306a\u308b\u305f\u3081\u3001\u963b\u5bb3\u3092\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u30b5\u30f3\u30d7\u30ebDNA\u6fc3\u5ea6\u304c\u6bd4\u8f03\u7684\u9ad8\u3044\u5834\u5408\uff085 ng\/\u03bcL\u4ee5\u4e0a\uff09\u306b\u304a\u3044\u3066\u3082Ct\u5024\u304c\u308f\u305a\u304b\u306b\u5927\u304d\u304f\u306a\u308b\u50be\u5411\u306b\u3042\u308a\u307e\u3059\u3002\u306a\u305c\u306a\u3089\u9ad8\u3044DNA\u6fc3\u5ea6\u306b\u3088\u308a\u53cd\u5fdc\u304c\u98fd\u548c\u3057\u3066\u3057\u307e\u3063\u3066\u3044\u308b\u305f\u3081\u3067\u3059\u3002\u3053\u306e\u305f\u3081\u30a4\u30f3\u30bf\u30fc\u30ca\u30ebPCR\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306eCt\u5024\u3068DNA\u6fc3\u5ea6\u306f\u540c\u6642\u306b\u8a55\u4fa1\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\u305f\u3068\u3048\u307050 ng\/\u03bcL\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u963b\u5bb3\u304c\u307f\u3089\u308c\u308b\u5834\u5408\u306f\u3001\u4ed6\u306e\u9ad8\u6fc3\u5ea6DNA\u306e\u30b5\u30f3\u30d7\u30eb\u3084\u540c\u6fc3\u5ea6\u306e\u30b5\u30a4\u30ba\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306e\u30a4\u30f3\u30bf\u30fc\u30ca\u30ebPCR\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306eCt\u5024\u3068\u306e\u6bd4\u8f03\u304c\u91cd\u8981\u3067\u3059\u3002
\n\"\"
\n\u524d\u8ff0\u3057\u305f\u3088\u3046\u306b\u3001\u5206\u89e3\u3082\u963b\u5bb3\u3082\u5171\u306b\u5927\u304d\u3044DNA\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u5206\u89e3\u306a\u306e\u304b\u963b\u5bb3\u306a\u306e\u304b\u3092\u8a55\u4fa1\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u305f\u3068\u3048\u3070\u3001\u5206\u89e3\u6307\u6570\u304c\u9ad8\u304f\u3001\u30a4\u30f3\u30bf\u30fc\u30ca\u30ebPCR\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306eCt\u5024\u304c\u7406\u60f3\u7684\u3067\u3042\u308c\u3070\u3001\u5206\u89e3\u306e\u307f\u304c\u751f\u3058\u3066\u3044\u308b\u3068\u8a55\u4fa1\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u5206\u89e3\u6307\u6570\u3001\u30a4\u30f3\u30bf\u30fc\u30ca\u30ebPCR\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306eCt\u5024\u304c\u3068\u3082\u306b\u9ad8\u3044\u5834\u5408\u306f\u3001\u5206\u89e3\u3068\u963b\u5bb3\u306e\u4e21\u65b9\u306e\u53ef\u80fd\u6027\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3072\u3069\u3044\u963b\u5bb3\u304c\u307f\u3089\u308c\u308b\u30b5\u30f3\u30d7\u30eb\u306b\u3064\u3044\u3066\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u5e0c\u91c8\u3084\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u306a\u3069\u306e\u51e6\u7406\u3092\u3057\u3066\u3001\u963b\u5bb3\u3092\u9664\u304f\u64cd\u4f5c\u3092\u5b9f\u65bd\u3057\u305f\u5f8c\u306b\u518d\u5ea6\u5b9a\u91cf\u3092\u3057\u3001\u5206\u89e3\u304c\u751f\u3058\u3066\u3044\u308b\u304b\u3092\u8a55\u4fa1\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002
\n\"\"<\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u30fbQuantifiler Trio Quantification Kit\u306fDNA\u6fc3\u5ea6\u306b\u52a0\u3048\u3001\u5206\u89e3\u3001\u963b\u5bb3\u306e\u60c5\u5831\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u308b\uff01
\n\u30fb\u3053\u308c\u3089\u306e\u60c5\u5831\u3092\u8003\u616e\u3057\u3066\u6b21\u306eSTR\u5897\u5e45\u306e\u30b9\u30c6\u30c3\u30d7\u3078\u6700\u9069\u306a\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3092\u9078\u629e\u3067\u304d\u308b\uff01<\/p>\n

\u305d\u306e\u4ed6\u6cd5\u533b\u5b66\u95a2\u9023\u306e\u60c5\u5831\u306b\u3064\u3044\u3066\u306f\u305c\u3072\u3053\u3061\u3089\u306e\u30b5\u30a4\u30c8\u3082\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002
\nwho work with bone, and use real-time PCR analysis\u00bb<\/a><\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u76ee\u7684\u304a\u3088\u3073\u305d\u306e\u624b\u7d9a\u304d\u4e0a\u3067\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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