{"id":8443,"date":"2021-01-29T11:25:39","date_gmt":"2021-01-29T02:25:39","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=8443"},"modified":"2024-07-04T13:13:02","modified_gmt":"2024-07-04T04:13:02","slug":"protein_expression_mb_bid_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein_expression_mb_bid_ts_1\/","title":{"rendered":"\u3042\u306a\u305f\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3068\u3063\u3066\u6700\u9069\u306a\u767a\u73fe\u7cfb\u306f\uff1f"},"content":{"rendered":"

\u306f\u3058\u3081\u306b<\/h2>\n

\u7d44\u307f\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306f\u3001\u591a\u304f\u306e\u7814\u7a76\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u306b\u304a\u3044\u3066\u6b20\u304b\u305b\u306a\u3044\u6280\u8853\u306e\u4e00\u3064\u3067\u3059\u3002\u54fa\u4e73\u985e\u7d30\u80de\u3001\u6606\u866b\u7d30\u80de\u3001\u9175\u6bcd\u3001\u5927\u8178\u83cc\u3092\u7528\u3044\u305f\u7cfb\u3001\u304a\u3088\u3073\u7121\u7d30\u80de\u767a\u73fe\u7cfb\u306a\u3069\u3055\u307e\u3056\u307e\u306a\u767a\u73fe\u7cfb\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u3059\u3079\u3066\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u6700\u9069\u306a\u767a\u73fe\u7cfb\u306f\u7121\u304f\u3001\u4f7f\u7528\u7528\u9014\u3001\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5408\u308f\u305b\u3066\u4f7f\u3044\u5206\u3051\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u4ee5\u4e0b\u306e\u8868\u306f\u5404\u767a\u73fe\u7cfb\u306e\u7279\u9577\u3001\u8ab2\u984c\u3092\u307e\u3068\u3081\u305f\u3082\u306e\u3067\u3059\u3002\u5b9f\u969b\u306b\u8a66\u3057\u3066\u307f\u306a\u3044\u3068\u308f\u304b\u3089\u306a\u3044\u3053\u3068\u3082\u3044\u307e\u3060\u591a\u304f\u3042\u308a\u307e\u3059\u304c\u3001\u6700\u9069\u306a\u767a\u73fe\u7cfb\u3092\u7d5e\u308a\u8fbc\u3080\u76ee\u5b89\u306b\u306a\u308b\u306f\u305a\u3067\u3059\u3002\u307e\u305f\u3001\u8868\u306e\u5f8c\u306b\u767a\u73fe\u7cfb\u3054\u3068\u306e\u73fe\u5728\u304a\u52e7\u3081\u306e\u88fd\u54c1\u3001\u304a\u3088\u3073\u95a2\u9023\u3059\u308b\u6280\u8853\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u306e\u3067\u4f75\u305b\u3066\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u5404\u767a\u73fe\u7cfb\u306b\u3064\u3044\u3066\uff5e\u304a\u52e7\u3081\u306e\u88fd\u54c1\u60c5\u5831<\/h2>\n

\u54fa\u4e73\u985e\u7d30\u80de\u767a\u73fe\u7cfb<\/h3>\n

\u7279\u306b\u54fa\u4e73\u985e\u7531\u6765\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3067\u306f\u6d3b\u6027\u3092\u6301\u3064\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5f97\u3089\u308c\u308b\u53ef\u80fd\u6027\u304c\u3082\u3063\u3068\u3082\u9ad8\u3044\u3068\u8a00\u3048\u307e\u3059\u304c\u3001\u4e00\u822c\u7684\u306b\u30b3\u30b9\u30c8\u304c\u9ad8\u304f\u3001\u767a\u73fe\u30ec\u30d9\u30eb\u304c\u4f4e\u3044\u3053\u3068\u304c\u8ab2\u984c\u3067\u3059\u3002\u305f\u3060\u3057\u3001\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u3042\u305f\u308a\u306e\u767a\u73fe\u91cf\u304c\u5c11\u306a\u3044\u305f\u3081\u7cbe\u88fd\u306b\u624b\u9593\u304c\u304b\u304b\u308a\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u3044\u304b\u306b\u767a\u73fe\u30ec\u30d9\u30eb\u3092\u4e0a\u3052\u308b\u304b\u304c\u30dd\u30a4\u30f3\u30c8\u3068\u306a\u308a\u307e\u3059\u3002\u307e\u305a\u306f\u901a\u5e38\u9ad8\u767a\u73fe\u3092\u671b\u3081\u308b\u30d9\u30af\u30bf\u30fc\u306e\u9078\u629e\u304c\u809d\u8981\u3067\u3059\u3002\u73fe\u5728\u3001\u5f53\u793e\u3067\u306f\u4ee5\u4e0b\u306e\u30d9\u30af\u30bf\u30fc\u304c\u9ad8\u767a\u73fe\u3092\u671f\u5f85\u3067\u304d\u307e\u3059\u3002<\/p>\n

pcDNA\u21223.4 TOPO\u2122 TA Cloning Kit<\/a><\/p>\n

\u307e\u305f\u3001\u6d6e\u904a\u57f9\u990a\u7cfb\u3067\u6bd4\u8f03\u7684\u9ad8\u767a\u73fe\u306a\u30b7\u30b9\u30c6\u30e0\u304c\u51fa\u59cb\u3081\u3066\u3044\u307e\u3059\uff08\u6297\u4f53\u3092g\/L\u30b9\u30b1\u30fc\u30eb\uff09\u3002\u6d6e\u904a\u7d30\u80de\u7528\u306e\u8a2d\u5099\u3092\u6e96\u5099\u3057\u3066\u3044\u305f\u3060\u304f\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u305c\u3072\u4e00\u5ea6\u304a\u8a66\u3057\u304f\u3060\u3055\u3044\u3002<\/p>\n

Gibco\u2122 Expi293\u2122Expression System<\/a>
\nGibco\u2122 ExpiCHO\u2122 Expression System<\/a><\/p>\n

\u307e\u305f\u3001\u54fa\u4e73\u985e\u7d30\u80de\u767a\u73fe\u7cfb\u306f\u7cd6\u9396\u4ed8\u52a0\u304c\u8d77\u3053\u308b\u3053\u3068\u304c\u30e1\u30ea\u30c3\u30c8\u306e\u4e00\u3064\u3067\u3059\u304c\u3001\u69cb\u9020\u89e3\u6790\u3092\u884c\u3046\u5834\u5408\u306b\u90aa\u9b54\u306b\u306a\u308b\u3053\u3068\u3082\u3042\u308b\u3068\u601d\u3044\u307e\u3059\u3002\u305d\u3093\u306a\u5834\u5408\u306fGibco\u2122 Expi293F\u2122 GnTI- Cells<\/a>\u3092\u3054\u691c\u8a0e\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u6606\u866b\u7d30\u80de\u767a\u73fe\u7cfb<\/h3>\n

\u54fa\u4e73\u985e\u7d30\u80de\u767a\u73fe\u7cfb\u306b\u6b21\u3044\u3067\u9ad8\u6d3b\u6027\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5f97\u3089\u308c\u3001\u304b\u3064\u3001\u3046\u307e\u304f\u3044\u3051\u3070\u767a\u73fe\u30ec\u30d9\u30eb\u304c\u9ad8\u3044\u3068\u3044\u3046\u5229\u70b9\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u901a\u5e38\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9\u3092\u4f7f\u7528\u3059\u308b\u305f\u3081\u3001\u305d\u306e\u624b\u9593\u3068\u6642\u9593\u304c\u554f\u984c\u3068\u8003\u3048\u3089\u308c\u308b\u65b9\u304c\u591a\u3044\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u4ee5\u4e0b\u306e\u88fd\u54c1\u3082\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u5404\u30b9\u30c6\u30c3\u30d7\u306e\u52b9\u7387\u3092\u4e0a\u3052\u308b\u3053\u3068\u306b\u3088\u308a\u30a6\u30a4\u30eb\u30b9\u5897\u5e45\u30b9\u30c6\u30c3\u30d7\u3092\u7701\u304d\u3001\u6700\u77ed1\u9031\u9593\u7a0b\u5ea6\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

Gibco\u2122 ExpiSf9\u2122 Baculovirus Expression System<\/a>
\n\u203b\u5404\u793e\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9\u306e\u30b2\u30ce\u30e0DNA\u3067\u3042\u308bBacmid\u4f5c\u6210\u65b9\u6cd5\u3092\u5de5\u592b\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u4e0a\u8a18\u30b7\u30b9\u30c6\u30e0\u3067\u306f\u5c02\u7528\u306e\u5927\u8178\u83cc\u5185\u3067Bacmid\u4f5c\u6210\u3092\u884c\u3046\u3001Bac-to-Bac\u2122\u30b7\u30b9\u30c6\u30e0\u3092\u63a1\u7528\u3057\u3066\u3044\u307e\u3059\u3002
\n\u3061\u306a\u307f\u306b\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9\u3092\u4f7f\u7528\u3057\u306a\u3044\u7cfb\u3082\u3042\u308a\u307e\u3059\u3002\u767a\u73fe\u30ec\u30d9\u30eb\u306f\u4e00\u822c\u7684\u306b\u30d0\u30ad\u30e5\u30ed\u30a6\u30a4\u30eb\u30b9\u3092\u4f7f\u7528\u3057\u305f\u6642\u3088\u308a\u3082\u4f4e\u304f\u306a\u308a\u307e\u3059\u304c\u3001\u54fa\u4e73\u985e\u7d30\u80de\u767a\u73fe\u7cfb\u3068\u540c\u3058\u3088\u3046\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u3088\u308a\u4e00\u904e\u6027\u3001\u5b89\u5b9a\u767a\u73fe\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n

Invitrogen\u2122 InsectSelect\u2122\u30b7\u30b9\u30c6\u30e0<\/a>\uff08Sf9\u7d30\u80de\u306a\u3069\u4f7f\u7528\uff09<\/p>\n

\u9175\u6bcd\u767a\u73fe\u7cfb<\/h3>\n

\u5358\u7d30\u80de\u751f\u7269\u3067\u3059\u304c\u771f\u6838\u751f\u7269\u3067\u3059\u306e\u3067\u3001\u5c0f\u80de\u4f53\u3092\u6301\u3064\u305f\u3081\u5206\u6ccc\u767a\u73fe\u304c\u884c\u3048\u307e\u3059\u3002\u4e3b\u306bSaccharomyces cerevisiae\u3092\u4f7f\u3063\u305f\u7cfb\u3068Pichia pastoris\u3092\u4f7f\u3063\u305f\u7cfb\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5927\u91cf\u767a\u73fe\u306b\u306f\u4e3b\u306b\u5f8c\u8005\uff08Pichia\uff09\u304c\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002<\/strong>\u5927\u8178\u83cc\u3088\u308a\u306f\u5c11\u3057\u624b\u9593\u304c\u304b\u304b\u308a\u307e\u3059\u304c\u3001Pichia\u3060\u3068\u304b\u306a\u308a\u9ad8\u767a\u73fe\u3082\u671f\u5f85\u3067\u304d\u307e\u3059\u3002\u9175\u6bcd\u306e\u5834\u5408\u3001\u7d44\u307f\u63db\u3048\u4f53\u306e\u9078\u5225\u306b\u6804\u990a\u8981\u6c42\u6027\u3092\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u591a\u304f\u305d\u306e\u5834\u5408\u6700\u5c0f\u57f9\u5730\u3092\u4f7f\u7528\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u5927\u8178\u83cc\u3068\u540c\u69d8\u306b\u6297\u751f\u7269\u8cea\u8010\u6027\u3067\u9078\u5225\u3059\u308b\u30b7\u30b9\u30c6\u30e0\u3082\u6e96\u5099\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

Invitrogen\u2122 EasySelect\u2122 Pichia Expression Kit<\/a><\/p>\n

\u5927\u8178\u83cc\u767a\u73fe\u7cfb<\/h3>\n

\u3068\u306b\u304b\u304f\u5b89\u4fa1\u3067\u9ad8\u767a\u73fe\u3067\u304d\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u306e\u3067\u3001\u307e\u305a\u306f\u4e00\u5ea6\u8a66\u3057\u3066\u307f\u308b\u4fa1\u5024\u304c\u3042\u308b\u3068\u601d\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u4e0d\u6eb6\u5316\u3057\u3066\u3082\u3044\u3044\u5834\u5408\u306a\u3069\u306f\u6700\u9069\u3060\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u305f\u3060\u3057\u3001\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u308a\u5c01\u5165\u4f53\u3092\u53ef\u6eb6\u5316\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd\u306a\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n

Thermo Scientific\u2122 Pierce\u2122 Protein Refolding Kit<\/a><\/p>\n

\u306a\u304a\u3001\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u3088\u3046\u306b\u3082\u3068\u3082\u3068\u4e0d\u6eb6\u6027\u306e\u3082\u306e\u3001\u304a\u3088\u3073\u5206\u6ccc\u30bf\u30f3\u30d1\u30af\u8cea\u306a\u3069\u306b\u306f\u901a\u5e38\u4e0d\u5411\u304d\u3067\u3059\uff08\u4e00\u90e8\u30d9\u30af\u30bf\u30fc\u3067\u306f\u53ef\u80fd\u306a\u5834\u5408\u3082\u3042\u308a\u307e\u3059\uff09\u304c\u3001\u7d30\u80de\u8cea\u5c40\u5728\u306e\u53ef\u6eb6\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5834\u5408\u3001\u8a66\u3057\u3066\u307f\u308b\u4fa1\u5024\u304c\u5341\u5206\u3042\u308b\u3068\u601d\u3044\u307e\u3059\u3002
\n\u3055\u307e\u3056\u307e\u306a\u30e1\u30fc\u30ab\u30fc\u304b\u3089\u540c\u3058\u3088\u3046\u306a\u30b7\u30b9\u30c6\u30e0\u304c\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001IPTG\u8a98\u5c0e\u3092\u304b\u3051\u308b\u524d\u304b\u3089\u767a\u73fe\u3057\u3066\u3057\u307e\u3046\u554f\u984c\u3067\u3001\u767a\u73fe\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e0d\u6eb6\u5316\u3001\u6bd2\u6027\u306b\u3088\u308b\u53ce\u91cf\u4f4e\u4e0b\u306e\u539f\u56e0\u306b\u306a\u308b\u8a98\u5c0e\u6f0f\u308c\u306e\u554f\u984c\u3092\u89e3\u6d88\u3067\u304d\u308bBL21-AI\u2122\u682a\u306b\u3064\u3044\u3066\u306f\u4e00\u8003\u306e\u4fa1\u5024\u304c\u3042\u308b\u3068\u601d\u3044\u307e\u3059\uff08\u30a2\u30e9\u30d3\u30ce\u30fc\u30b9\u8a98\u5c0e\u7cfb\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u4e00\u822c\u7684\u306apET\u7cfb\u30d9\u30af\u30bf\u30fc\u3092\u4f7f\u7528\u53ef\u80fd\u3067\u3059\uff09\u3002<\/p>\n

Invitrogen\u2122 BL21-AI\u2122 One Shot\u2122 Chemically Competent E. coli<\/a><\/p>\n

\u306a\u304a\u3001\u4e00\u822c\u7684\u306b\u306f\u30bf\u30b0\u7121\u3057\u3088\u308a\u306f\u3001\u77ed\u3044\u3082\u306e\u3067\u3082N\u672b\u7aef\u5074\u306b\u4ed8\u3051\u305f\u65b9\u304c\u767a\u73fe\u6210\u529f\u7387\u306f\u9ad8\u3044\u3088\u3046\u3067\u3059\u3002\u5f53\u793eGateway\u30b7\u30b9\u30c6\u30e0\u3092\u4f7f\u3048\u3070\u5404\u7a2e\u30bf\u30b0\u3068\u306e\u76f8\u6027\u3092\u8abf\u3079\u308b\u306e\u306b\u4fbf\u5229\u3067\u3059\uff08\u305d\u306e\u4ed6\u306e\u95a2\u9023\u6280\u8853\u53c2\u7167\uff09\u3002\u307e\u305f\u3001\u4eba\u5de5\u907a\u4f1d\u5b50\u5408\u6210\u3092\u5229\u7528\u3057\u3001\u30b3\u30c9\u30f3\u306e\u6700\u9069\u5316\u3092\u884c\u3046\u306e\u3082\u9ad8\u53ce\u91cf\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\u6709\u52b9\u306a\u5834\u5408\u304c\u591a\u3044\u3088\u3046\u3067\u3059\uff08\u305d\u306e\u4ed6\u306e\u95a2\u9023\u6280\u8853\u53c2\u7167\uff09\u3002<\/p>\n

\u85fb\u985e\u767a\u73fe\u7cfb<\/h3>\n

\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5927\u91cf\u306b\u4f5c\u308a\u305f\u3044\u5834\u5408\u306b\u4f7f\u7528\u3055\u308c\u308b\u30b1\u30fc\u30b9\u306f\u5c11\u306a\u304f\u3001\u5149\u5408\u6210\u306a\u3069\u3001\u690d\u7269\u7279\u6709\u306e\u6a5f\u80fd\u306b\u95a2\u3059\u308b\u7814\u7a76\u306b\u30e2\u30c7\u30eb\u751f\u7269\u3068\u3057\u3066\u4f7f\u7528\u3055\u308c\u308b\u306e\u304c\u307b\u3068\u3093\u3069\u3060\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u305d\u306e\u4ed6\u30d0\u30a4\u30aa\u71c3\u6599\u958b\u767a\u306e\u30d1\u30a4\u30ed\u30c3\u30c8\u5b9f\u9a13\u306b\u4f7f\u7528\u3055\u308c\u308b\u3053\u3068\u3082\u3042\u308a\u305d\u3046\u3067\u3059\u3002
\n\u5f53\u793e\u306f\u3082\u3063\u3068\u3082\u3088\u304f\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u682a\u306e\u4e00\u3064\u3067\u3042\u308b\u3001\u30e9\u30f3\u85fbSynechococcus elongatus\u304a\u3088\u3073\u30af\u30e9\u30df\u30c9\u30e2\u30ca\u30b9Chlamydomonas reinhardtii 137c\u3067\u4f7f\u7528\u53ef\u80fd\u306a\u57f9\u5730\u3068\u30d9\u30af\u30bf\u30fc<\/a>\u306e\u8ca9\u58f2\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u306a\u304a\u3001\u54fa\u4e73\u985e\u7531\u6765\u306e\u907a\u4f1d\u5b50\u3092\u767a\u73fe\u3055\u305b\u308b\u5834\u5408\u306f\u3001\u5869\u57fa\u914d\u5217\u306e\u6700\u9069\u5316\u304c\u6709\u52b9\u306a\u5834\u5408\u304c\u591a\u3044\u3088\u3046\u3067\u3059\u3002\u4eba\u5de5\u907a\u4f1d\u5b50\u5408\u6210\u3092\u5229\u7528\u3057\u3001\u30b3\u30c9\u30f3\u306e\u6700\u9069\u5316\u3092\u884c\u3046\u306e\u3082\u9ad8\u53ce\u91cf\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\u6709\u52b9\u306a\u5834\u5408\u304c\u591a\u3044\u3088\u3046\u3067\u3059\uff08\u305d\u306e\u4ed6\u306e\u95a2\u9023\u6280\u8853\u53c2\u7167\uff09\u3002<\/p>\n

\u7121\u7d30\u80de\u767a\u73fe\u7cfb<\/h3>\n

\u30cf\u30a4\u30b3\u30b9\u30c8\u3067\u3042\u308b\u3053\u3068\u306f\u5909\u308f\u308a\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u6bd4\u8f03\u7684\u9ad8\u767a\u73fe\u304c\u671f\u5f85\u3067\u304d\u308b\u30b7\u30b9\u30c6\u30e0\u3068\u3057\u3066\u3001\u5927\u8178\u83cc\u3001\u5c0f\u9ea6\u80da\u82bd\u3001\u54fa\u4e73\u985e\u7d30\u80de\u3001\u6606\u866b\u7d30\u80de\u3092\u4f7f\u3063\u305f\u7cfb\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u7279\u306b\u69cb\u9020\u89e3\u6790\u7528\u306b\u5b89\u5b9a\u540c\u4f4d\u4f53\u306a\u3069\u306e\u30e9\u30d9\u30eb\u3092\u5165\u308c\u3089\u308c\u305f\u3044\u5834\u5408\u306b\u306f\u691c\u8a0e\u3055\u308c\u308b\u4fa1\u5024\u304c\u3042\u308b\u3068\u601d\u3044\u307e\u3059\u3002\u307e\u305f\u300196 well\u30d7\u30ec\u30fc\u30c8\u306a\u3069\u3067\u3082\u884c\u3048\u3066\u3001\u57fa\u672c\u7684\u306b\u77ed\u6642\u9593\u3067\u5b8c\u4e86\u3059\u308b\u305f\u3081\u3001\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u30d5\u30ec\u30f3\u30c9\u30ea\u30fc\u306a\u767a\u73fe\u7cfb\u3060\u3068\u8a00\u3048\u307e\u3059\u3002\u307e\u305f\u3001\u767a\u73fe\u81ea\u4f53\u306f\u7d44\u307f\u63db\u3048\u5b9f\u9a13\u306b\u306f\u3042\u305f\u308a\u307e\u305b\u3093\u3002\u5de5\u592b\u3059\u308c\u3070\u5168\u884c\u7a0b\u3092\u975e\u7d44\u307f\u63db\u3048\u5b9f\u9a13\u3068\u3057\u3066\u884c\u3046\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002
\n\u5f53\u793e\u3067\u306f\u5927\u8178\u83cc\u304a\u3088\u3073\u54fa\u4e73\u985e\u7d30\u80de\u7531\u6765\u306e\u62bd\u51fa\u6db2\u3092\u4f7f\u7528\u3057\u305f\u7cfb\u3092\u8ca9\u58f2\u3057\u3066\u3044\u307e\u3059\u3002\u3084\u306f\u308a\u54fa\u4e73\u985e\u7531\u6765\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306b\u306f\u54fa\u4e73\u985e\u7531\u6765\u306e\u7cfb\u306e\u65b9\u304c\u76f8\u6027\u306f\u3044\u3044\u3088\u3046\u3067\u3059\u3002<\/p>\n

\u5927\u8178\u83cc\u7121\u7d30\u80de\u767a\u73fe\u7cfb\uff1aInvitrogen\u2122 Expressway\u2122 Cell-Free Expression System<\/a>
\n\u54fa\u4e73\u985e\u7121\u7d30\u80de\u767a\u73fe\u7cfb\uff1aThermo Scientific\u2122 1-Step Human Coupled IVT Kit \u2013 DNA<\/a><\/p>\n

<\/h2>\n

\u305d\u306e\u4ed6\u306e\u95a2\u9023\u6280\u8853<\/h2>\n

\u5b9f\u9a13\u3092\u59cb\u3081\u308b\u969b\u3001\u307e\u305a\u306f\u3069\u306e\u3088\u3046\u306a\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3092\u4f7f\u7528\u3059\u308b\u304b\u3092\u8003\u3048\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u969b\u3001\u4ee5\u4e0b\u306e\u691c\u7d22\u30b5\u30a4\u30c8\u304c\u4fbf\u5229\u3067\u3059\u3002<\/p>\n

Vector Selection Tool<\/a><\/p>\n

\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u6e96\u5099\u306b\u306f\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u6280\u8853\u304c\u5fc5\u8981\u3067\u3059\u3002\u3053\u308c\u304b\u3089\u59cb\u3081\u3089\u308c\u308b\u65b9\u306b\u306f\u4ee5\u4e0b\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u304c\u304a\u52e7\u3081\u3067\u3059\u3002<\/p>\n

\u521d\u3081\u3066\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0<\/a><\/p>\n

\u307e\u305f\u3001\u4e00\u304b\u3089\u8a66\u85ac\u306a\u3069\u3092\u305d\u308d\u3048\u308b\u5fc5\u8981\u304c\u3042\u308b\u5834\u5408\u306a\u3069\u3001\u4eba\u5de5\u907a\u4f1d\u5b50\u5408\u6210\u3067\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3092\u4f5c\u3063\u3066\u3057\u307e\u3046\u306e\u3082\u4e00\u624b\u3067\u3059\u3002\u5f53\u793e\u306e\u591a\u304f\u306e\u30d9\u30af\u30bf\u30fc\u306f\u672c\u30b5\u30fc\u30d3\u30b9\u3067\u306f\u7121\u511f\u3067\uff08\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u4ee3\u306f\u5fc5\u8981\uff09\u3054\u5229\u7528\u3044\u305f\u3060\u3051\u307e\u3059\u306e\u3067\u4fbf\u5229\u3067\u3059\u3002\u307e\u305f\u3001\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8<\/a>\u3078\u304a\u554f\u3044\u5408\u308f\u305b\u3044\u305f\u3060\u3051\u308c\u3070\u9069\u5207\u306a\u30d9\u30af\u30bf\u30fc\u306e\u9078\u3073\u65b9\u306a\u3069\u3054\u6848\u5185\u53ef\u80fd\u3067\u3059\u3002<\/p>\n

GeneArt\u907a\u4f1d\u5b50\u5408\u6210\u30b5\u30fc\u30d3\u30b9<\/a><\/p>\n

\u3055\u3089\u306b\u3001\u7279\u306b\u5019\u88dc\u907a\u4f1d\u5b50\u306e\u7531\u6765\u751f\u7269\u3068\u767a\u73fe\u7528\u306e\u30db\u30b9\u30c8\u751f\u7269\u304c\u9032\u5316\u7684\u306b\u96e2\u308c\u3066\u3044\u308b\u5834\u5408\u306a\u3069\u3001\u6700\u9069\u5316\u304c\u975e\u5e38\u306b\u6709\u52b9\u306a\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u30d2\u30c8\u306e\u907a\u4f1d\u5b50\u3092\u5927\u8178\u83cc\u3084\u85fb\u985e\u306a\u3069\u3067\u767a\u73fe\u3055\u305b\u308b\u5834\u5408\u306f\u6700\u9069\u5316\u3092\u884c\u3046\u305f\u3081\u306b\u907a\u4f1d\u5b50\u3092\u4eba\u5de5\u5408\u6210\u3059\u308b\u3068\u3044\u3046\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u610f\u5916\u3068\u30d2\u30c8\u306e\u907a\u4f1d\u5b50\u3092\u30d2\u30c8\u306e\u7d30\u80de\u3067\u767a\u73fe\u3055\u305b\u308b\u5834\u5408\u3067\u3082\u6700\u9069\u5316\u306b\u3088\u308a\u767a\u73fe\u91cf\u304c\u5897\u3048\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

GeneArt\u2122 GeneOptimizer\u2122<\/a><\/p>\n

\u3084\u306f\u308a\u3069\u306e\u767a\u73fe\u7cfb\u304c\u6700\u9069\u5316\u306f\u8a66\u3057\u3066\u307f\u306a\u3044\u3068\u308f\u304b\u3089\u306a\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u5927\u8178\u83cc\u767a\u73fe\u7cfb\u3067\u306f\u4e0d\u6eb6\u5316\u3057\u3066\u3057\u307e\u3044\u3001\u30bf\u30b0\u3092\u8907\u6570\u7a2e\u985e\u8a66\u3059\u5fc5\u8981\u304c\u3042\u308b\u3001\u3042\u308b\u3044\u306f\u5927\u8178\u83cc\u3092\u3042\u304d\u3089\u3081\u3066\u54fa\u4e73\u985e\u7d30\u80de\u767a\u73fe\u7cfb\u3092\u8a66\u3059\u306a\u3069\u3002Gateway\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3092\u5229\u7528\u3059\u308c\u3070\u3001\u5bfe\u8c61\u907a\u4f1d\u5b50\u306e\u4ed6\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3078\u306e\u4e57\u305b\u63db\u3048\u3092\u7c21\u4fbf\u306b\u884c\u3048\u307e\u3059\u3002<\/p>\n

Gateway\u30c6\u30af\u30ce\u30ed\u30b8\u30fc<\/a><\/p>\n

\u6700\u5f8c\u306b\u3001\u5b9f\u9a13\u306b\u4f7f\u7528\u3059\u308b\u7d44\u307f\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u767a\u73fe\u3059\u308b\u30b5\u30fc\u30d3\u30b9\u3082\u3054\u3056\u3044\u307e\u3059\u3002<\/p>\n

\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u53d7\u8a17\u30b5\u30fc\u30d3\u30b9<\/a><\/p>\n

\u305d\u306e\u4ed6\u3054\u4e0d\u660e\u70b9\u306a\u70b9\u306f\u304a\u6c17\u8efd\u306b\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8<\/a>\u307e\u3067\u3054\u9023\u7d61\u304f\u3060\u3055\u3044\u3002
\nTel: 0120-477-392\uff08\u30c0\u30a4\u30ec\u30af\u30c8\u756a\u53f76\uff09<\/p>\n

\u3010\u7121\u6599\u516c\u958b\u4e2d\u3011Protein Expression\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n

\u30bf\u30f3\u30d1\u30af\u767a\u73fe\u306e\u5b9f\u9a13\u3092\u691c\u8a0e\u3057\u3066\u3044\u308b\u65b9\u3084\u3001\u8a73\u7d30\u60c5\u5831\u3092\u78ba\u8a8d\u3057\u305f\u3044\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u306f\u3058\u3081\u306b 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