{"id":8486,"date":"2021-07-09T11:23:57","date_gmt":"2021-07-09T02:23:57","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=8486"},"modified":"2025-06-12T19:52:59","modified_gmt":"2025-06-12T10:52:59","slug":"fragment_analysis_ce_gsd_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/fragment_analysis_ce_gsd_ts_1\/","title":{"rendered":"\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u306e\u539f\u7406\u306b\u3064\u3044\u3066\u2013 Seq It Out\u2122\uff033"},"content":{"rendered":"<p>\u30ad\u30e3\u30d4\u30e9\u30ea\u96fb\u6c17\u6cf3\u52d5\u30b7\u30b9\u30c6\u30e0\u3092\u4f7f\u7528\u3057\u3066\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3092\u884c\u3046\u4e0a\u3067\u3001\u3069\u306e\u3088\u3046\u306b\u3057\u3066\u30c7\u30fc\u30bf\u3092\u691c\u51fa\u3057\u3066\u3044\u308b\u306e\u304b\u8003\u3048\u305f\u3053\u3068\u306f\u3042\u308a\u307e\u305b\u3093\u304b\u3002\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u306e\u539f\u7406\u306b\u3064\u3044\u3066\u5206\u304b\u308a\u3084\u3059\u304f\u8aac\u660e\u3057\u305f\u30d3\u30c7\u30aa\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u306e\u3067\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<br \/>\n\u30fb\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u89e3\u6790\u3092\u884c\u3063\u3066\u3044\u308b\u3051\u3069\u3001\u691c\u51fa\u539f\u7406\u306f\u8003\u3048\u305f\u3053\u3068\u304c\u306a\u304b\u3063\u305f<br \/>\n\u30fbSNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u306f\u3001\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3067\u3067\u304d\u308b\u306e\u304b\u77e5\u308a\u305f\u3044<\/p>\n<p><iframe loading=\"lazy\" title=\"How does Fragment Analysis work? \u2013 Seq It Out #3\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/-RzKFR-n5zs?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/p>\n<p>\u30d3\u30c7\u30aa\u306e\u5185\u5bb9\u306b\u3064\u3044\u3066\u4e0b\u8a18\u306b\u7c21\u5358\u306b\u307e\u3068\u3081\u3066\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u305c\u3072\u3054\u53c2\u8003\u306b\u306a\u3055\u3063\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>\u6614\u306f\u7d50\u69cb\u5927\u5909\u306a\u6280\u8853\u3060\u3063\u305f\uff1f<\/h2>\n<p>\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3068\u306f\u3001\u7a81\u7136\u5909\u7570\u691c\u51fa\u3001SNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u3001DNA\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u3001\u907a\u4f1d\u5b50\u30de\u30c3\u30d4\u30f3\u30b0\u3001\u9023\u9396\u89e3\u6790\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u4f7f\u7528\u3055\u308c\u308b\u907a\u4f1d\u5b50\u89e3\u6790\u624b\u6cd5\u5168\u822c\u306e\u3053\u3068\u3092\u6307\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u65b9\u6cd5\u306b\u3088\u308a\u3001\u3055\u307e\u3056\u307e\u306a\u75be\u60a3\u3084\u67d3\u8272\u4f53\u7570\u5e38\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<br \/>\n\u5f93\u6765\u306e\u65b9\u6cd5\u3067\u306f\u3001DNA\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306f\u30a2\u30ac\u30ed\u30fc\u30b9\u3084\u30dd\u30ea\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30b2\u30eb\u306a\u3069\u3092\u4f7f\u7528\u3057\u3066\u30b5\u30a4\u30ba\u3054\u3068\u306b\u5206\u96e2\u3057\u3001\u30b5\u30a4\u30ba\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u6bd4\u8f03\u3059\u308b\u3053\u3068\u3067\u6c7a\u5b9a\u3057\u3066\u3044\u307e\u3057\u305f\u3002\u30a8\u30c1\u30b8\u30a6\u30e0\u30d6\u30ed\u30de\u30a4\u30c9\u3084\u653e\u5c04\u6027\u540c\u4f4d\u5143\u7d20\u3092\u4f7f\u7528\u3057\u3001\u30b9\u30e9\u30d6\u30b2\u30eb\u3067\u306e\u96fb\u6c17\u6cf3\u52d5\u4e2d\u307e\u305f\u306f\u96fb\u6c17\u6cf3\u52d5\u5f8c\u306bDNA\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u3092\u6a19\u8b58\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u53ef\u8996\u5316\u3057\u691c\u51fa\u3057\u3066\u3044\u307e\u3057\u305f\u3002\u73fe\u5728\u3067\u306f\u3001\u5b89\u5168\u306a\u86cd\u5149\u8272\u7d20\u3092\u4f7f\u7528\u3057\u3001\u3088\u308a\u9ad8\u3044\u89e3\u50cf\u5ea6\u3068\u7cbe\u5ea6\u3067\u5206\u96e2\u304c\u53ef\u80fd\u306a\u30ad\u30e3\u30d4\u30e9\u30ea\u96fb\u6c17\u6cf3\u52d5\u30b7\u30b9\u30c6\u30e0\u3092\u4f7f\u7528\u3059\u308b\u624b\u6cd5\u304c\u4e3b\u6d41\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2>\u86cd\u5149\u691c\u51fa\u306e\u4ed5\u7d44\u307f<\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/sequencing\/sanger-sequencing.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7502_gsd00000_0so_blg_op_awa_og_s00_dne10_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30ad\u30e3\u30d4\u30e9\u30ea\u96fb\u6c17\u6cf3\u52d5\u30b7\u30b9\u30c6\u30e0<\/a>\u3067\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3092\u5b9f\u884c\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u76ee\u7684\u9818\u57df\u3092\u631f\u307f\u8fbc\u3080\u3088\u3046\u306b2\u672c\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u86cd\u5149\u8272\u7d20\u306fForward\u307e\u305f\u306fReverse\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u3069\u3061\u3089\u304b\u4e00\u65b9\u306b\u4ed8\u52a0\u3057\u307e\u3059\u3002<\/p>\n<p>PCR\u306b\u3088\u3063\u3066\u5897\u5e45\u3055\u308c\u305f\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306f\u30b5\u30a4\u30ba\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u6df7\u5408\u3057\u307e\u3059\u306e\u3067\u3001\u3053\u306e\u6642\u30b5\u30a4\u30ba\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u3067\u3042\u308b\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306e\u86cd\u5149\u8272\u7d20\u3068\u306f\u7570\u306a\u308b\u8272\u3067\u6a19\u8b58\u3055\u308c\u3066\u3044\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u86cd\u5149\u6a19\u8b58\u3055\u308c\u305fPCR\u7523\u7269\u3068\u30b5\u30a4\u30ba\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306f\u3001\u52d5\u96fb\u5b66\u7684\u306b\u30ad\u30e3\u30d4\u30e9\u30ea\u306b\u53d6\u308a\u8fbc\u307e\u308c\u307e\u3059\u3002\u96fb\u6975\u9593\u306b\u9ad8\u96fb\u5727\u304c\u5370\u52a0\u3055\u308c\u308b\u3068\u3001\u8ca0\u306b\u5e2f\u96fb\u3057\u305fDNA\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306f\u3001\u30dd\u30ea\u30de\u30fc\u3067\u6e80\u305f\u3055\u308c\u305f\u30ad\u30e3\u30d4\u30e9\u30ea\u5185\u90e8\u3092\u901a\u3063\u3066\u3001\u9670\u6975\u5074\u304b\u3089\u967d\u6975\u5074\u306b\u5411\u304b\u3063\u3066\u79fb\u52d5\u3057\u307e\u3059\u3002\u30dd\u30ea\u30de\u30fc\u306f\u7de9\u3044\u7db2\u76ee\u69cb\u9020\u3092\u3068\u3063\u3066\u304a\u308a\u3001DNA\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u304c\u30dd\u30ea\u30de\u30fc\u306e\u4e2d\u3092\u79fb\u52d5\u3059\u308b\u6642\u3001\u5c0f\u3055\u3044\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306f\u901f\u304f\u79fb\u52d5\u3057\u3001\u5927\u304d\u3044\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u307b\u3069\u9045\u304f\u306a\u308b\u305f\u3081\u3001\u30b5\u30a4\u30ba\u306b\u3088\u3063\u3066\u305d\u308c\u305e\u308c\u3092\u5206\u96e2\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u307e\u305f\u3001\u967d\u6975\u306b\u5230\u9054\u3059\u308b\u524d\u306b\u3001\u86cd\u5149\u6a19\u8b58\u3055\u308c\u305fDNA\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306f\u3001\u30ec\u30fc\u30b6\u30fc\u30d3\u30fc\u30e0\u3092\u901a\u904e\u3057\u307e\u3059\u3002\u30ec\u30fc\u30b6\u30fc\u30d3\u30fc\u30e0\u306b\u3088\u308a\u3001\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u4e0a\u306e\u8272\u7d20\u304c\u86cd\u5149\u3092\u767a\u3057\u3001CCD\u30ab\u30e1\u30e9\u304c\u86cd\u5149\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u3067\u3001\u96fb\u6c17\u6cf3\u52d5\u56f3\u306b\u30d4\u30fc\u30af\u3068\u3057\u3066\u8868\u793a\u3067\u304d\u307e\u3059\u3002<\/p>\n<h2>SNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u3082\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3067\u5bfe\u5fdc\u3067\u304d\u307e\u3059<\/h2>\n<p>\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3067\u4f7f\u7528\u3067\u304d\u308b\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306f\u305f\u304f\u3055\u3093\u3042\u308a\u307e\u3059\u304c\u30011\u3064\u7279\u6b8a\u306a\u7cfb\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\u305d\u308c\u306f\u3001\u4e00\u5869\u57fa\u591a\u578b\u307e\u305f\u306fSNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u3067\u3059\u3002<br \/>\nApplied Biosystems\u2122 SNaPshot\u2122 Multiplex\u30ad\u30c3\u30c8\u306f\u3001\u7570\u306a\u308b\u9577\u3055\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u4f7f\u7528\u3057\u3066\u3001\u6700\u592710\u500b\u306eSNP\u30de\u30fc\u30ab\u30fc\u3092\u540c\u6642\u306b\u89e3\u6790\u3067\u304d\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u300110\u500b\u306e\u7570\u306a\u308bSNP\u306b\u96a3\u63a5\u3059\u308b\u914d\u5217\u306b\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u3059\u308b\u3088\u3046\u306b\u8a2d\u8a08\u3057\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u3059\u308b\u3068\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u304b\u30891\u5869\u57fa\u3060\u3051\u306e\u4f38\u9577\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002\u3053\u306e\u6642\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u306e\u3059\u3050\u96a3\u306e\u92f3\u578b\u306b\u76f8\u88dc\u7684\u306a\u86cd\u5149\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\uff08ddNTP\uff09\u3092\u4ed8\u52a0\u3059\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u30024\u3064\u306eddNTP\uff08ddGTP\u3001ddATP\u3001ddTTP\u3001ddCTP\uff09\u306f\u305d\u308c\u305e\u308c\u3001\u7570\u306a\u308b\u8272\u7d20\u3067\u86cd\u5149\u6a19\u8b58\u3055\u308c\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u3059\u3079\u3066\u540c\u3058\u9577\u3055\u3067\u691c\u51fa\u3055\u308c\u3066\u3082\u53d6\u308a\u8fbc\u307e\u308c\u305fddNTP\u306e\u8272\u306b\u3088\u3063\u3066\u914d\u5217\u3092\u77e5\u308b\u3053\u3068\u304c\u53ef\u80fd\u306b\u306a\u308b\u306e\u3067\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30fb\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3082\u3001\u4eca\u3067\u306f\u5b89\u5168\u306a\u86cd\u5149\u8272\u7d20\u3092\u4f7f\u7528\u3059\u308b\u65b9\u6cd5\u304c\u30e1\u30a4\u30f3\u306b\u306a\u308a\u307e\u3057\u305f\u3002<br \/>\n\u30fbDNA\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306f\u3001\u30dd\u30ea\u30de\u30fc\u5185\u3092\u79fb\u52d5\u3059\u308b\u9593\u306b\u30b5\u30a4\u30ba\u5206\u96e2\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u30fbSNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u3082\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3067\u30ab\u30d0\u30fc\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u3055\u3089\u306b\u57fa\u790e\u7684\u306a\u30d3\u30c7\u30aa\u3092\u304a\u63a2\u3057\u306e\u65b9\u306f\u3001\u4ed6\u306e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/products-and-services\/promotions\/ask.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7502_gsd00000_0so_blg_op_awa_og_s00_dne10_Social_LAB\" target=\"_blank\" rel=\"noopener\">Seq It Out\u2122<\/a>\u3082\u305c\u3072\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ac\u30a4\u30c9\uff08\u65e5\u672c\u8a9e\u7248\uff09<\/h2>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/fragment-analysis-guide-download.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7502_gsd00000_0so_blg_op_awa_og_s00_dne10_Social_LAB\" target=\"_blank\" rel=\"noopener 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