{"id":856,"date":"2018-07-10T00:00:00","date_gmt":"2018-07-10T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ph-gradient\/"},"modified":"2023-12-28T13:57:41","modified_gmt":"2023-12-28T04:57:41","slug":"ph-gradient","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ph-gradient\/","title":{"rendered":"\u30a4\u30f3\u30bf\u30af\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u96e2\u306b\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3068pH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u306e\u3069\u3061\u3089\u3092\u9078\u629e\u3057\u307e\u3059\u304b\uff1f"},"content":{"rendered":"<p>\u904e\u53bb20\u5e74\u3067\u3001\u4f4e\u5206\u5b50\u533b\u85ac\u54c1\u306e\u958b\u767a\u304b\u3089\u9ad8\u5206\u5b50\u533b\u85ac\u54c1\u306e\u958b\u767a\u306b\u79fb\u884c\u304c\u9032\u3093\u3067\u3044\u307e\u3059\u3002\u73fe\u5728\u3001\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306f\u533b\u85ac\u54c1\u5e02\u5834\u306e\u4e0a\u4f4d\u534a\u5206\u4ee5\u4e0a\u3092\u5360\u3081\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u4e2d\u3001\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u958b\u767a\u304b\u3089\u54c1\u8cea\u7ba1\u7406\u306b\u304a\u3044\u3066\u3001\u30a4\u30aa\u30f3\u4ea4\u63db\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3092\u5229\u7528\u3057\u305f\u30a4\u30f3\u30bf\u30af\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u96e2\u304c\u5e83\u304f\u884c\u308f\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u306a\u304b\u3067\u3082\u3001<u>\u30c1\u30e3\u30fc\u30b8\u30d0\u30ea\u30a2\u30f3\u30c8\u306e\u5206\u6790\u306f\u3001\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u5b89\u5168\u6027\u3084\u52b9\u80fd\u3092\u78ba\u4fdd\u3059\u308b\u305f\u3081\u306b\u975e\u5e38\u306b\u91cd\u8981\u306a\u5206\u6790\u306e\u4e00\u3064\u3067\u3059\u3002<\/u><\/p>\n<p>\u4e00\u822c\u7684\u306b\u30c1\u30e3\u30fc\u30b8\u30d0\u30ea\u30a2\u30f3\u30c8\u306e\u5206\u6790\u306f\u3001\u30a4\u30aa\u30f3\u4ea4\u63db\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3057\u305f\u4ee5\u4e0b\u4e8c\u3064\u306e\u79fb\u52d5\u76f8\u3092\u5229\u7528\u3057\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8<\/li>\n<li>pH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8<\/li>\n<\/ul>\n<p>\u3053\u3053\u3067\u306f\u3001\u30c1\u30e3\u30fc\u30b8\u30d0\u30ea\u30a2\u30f3\u30c8\u306e\u5206\u6790\u3092\u884c\u3046\u969b\u306b\u91cd\u8981\u306a<strong>\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3068<\/strong><strong>pH<\/strong><strong>\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u306e\u9055\u3044<\/strong>\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<h4><span style=\"font-size: 12pt\"><strong>\u25a0\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8<\/strong><\/span><\/h4>\n<p>\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u5834\u5408\u306e\u30a4\u30f3\u30bf\u30af\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u3001\u4e00\u822c\u7684\u306b\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\uff08mAb\uff09\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3067\u306f\u3001\u3053\u306emAb\u3068\u30ca\u30c8\u30ea\u30a6\u30a4\u30aa\u30f3\uff08Na<sup>+<\/sup>\uff09\u304cIEX\uff08\u30a4\u30aa\u30f3\u4ea4\u63db\uff09\u56fa\u5b9a\u76f8\u306e\u30a4\u30aa\u30f3\u4ea4\u63db\u57fa\u3092\u596a\u3044\u5408\u3044\u307e\u3059\u3002\u5869\u6fc3\u5ea6\u304c\u9ad8\u304f\u306a\u308b\u306b\u3064\u308c\u3001\u30a4\u30aa\u30f3\u4ea4\u63db\u90e8\u4f4d\u306e\u4e89\u596a\u3067\u30ca\u30c8\u30ea\u30a6\u30e0\u30a4\u30aa\u30f3\u304cmAb\u306b\u52dd\u3063\u3066\u3044\u304f\u305f\u3081\u3001mAb\u306f\u6eb6\u51fa\u3057\u59cb\u3081\u307e\u3059\u3002\u5869\u30b0\u30e9\u30b8\u30a7\u30f3\u30c8\u3067\u306f\u3001\u3053\u306emAb\u306f\u30ab\u30e9\u30e0\u5168\u4f53\u306e\u9023\u7d9a\u7684\u306a\u76f8\u4e92\u4f5c\u7528\u3092\u901a\u3057\u3066\u6eb6\u51fa\u3055\u308c\u308b\u305f\u3081\u3001\u30ab\u30e9\u30e0\u306e\u9577\u3055\u304c\u9577\u3044\u307b\u3069\u5206\u96e2\u304c\u826f\u597d\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<h4><span style=\"font-size: 12pt\"><strong>\u25a0pH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8<\/strong><\/span><\/h4>\n<p>pH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u3001mAb\u306f\u56fa\u5b9a\u76f8\u306e\u30a4\u30aa\u30f3\u4ea4\u63db\u57fa\u306b\u7d50\u5408\u3057\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001pH\u3092\u6b21\u7b2c\u306b\u4e0a\u6607\u3055\u305b\u3066\u3044\u304d\u307e\u3059\u3002mAb\u306e\u96fb\u8377\u304c\u7b49\u96fb\u70b9\uff08pI\uff09\u306b\u304a\u3044\u3066\u5168\u304f\u30c1\u30e3\u30fc\u30b8\u3092\u3082\u305f\u306a\u3044\u72b6\u614b\uff08\u30cb\u30e5\u30fc\u30c8\u30e9\u30eb\uff09\u306b\u306a\u308b\u3068\u3001mAb\u306f\u904a\u96e2\u3057\u3001\u6eb6\u51fa\u3057\u307e\u3059\u3002<strong>\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3068\u306f\u7570\u306a\u308a\u3001\u3053\u308c\u4ee5\u964d\u306f\u56fa\u5b9a\u76f8\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u304c\u767a\u751f\u3057\u306a\u3044\u305f\u3081\u3001\u30ab\u30e9\u30e0\u306e\u9577\u3055\u306f\u5206\u96e2\u306b\u307b\u3068\u3093\u3069\u5f71\u97ff\u3057\u307e\u305b\u3093\u3002<\/strong><\/p>\n<p>&nbsp;<\/p>\n<h2><strong>\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3001\u305d\u308c\u3068\u3082<\/strong><strong>pH<\/strong><strong>\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\uff1f<\/strong><\/h2>\n<p><strong>\u00a0<\/strong><\/p>\n<p>\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u306f\u4e00\u822c\u7684\u3067\u3001\u591a\u304f\u306e\u4eba\u304c\u4f7f\u3044\u6163\u308c\u3066\u3044\u3066\u3001\u7279\u306b\u554f\u984c\u306a\u304f\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u4eca\u306e\u3084\u308a\u65b9\u306b\u56fa\u57f7\u3059\u308b\u3053\u3068\u304c\u6700\u826f\u306a\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n<p>\u305d\u3053\u3067\u3001\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3068pH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u306e\u8003\u3048\u3089\u308c\u308b\u6b20\u70b9\u3092\u307e\u3068\u3081\u3066\u307f\u307e\u3057\u305f\uff08\u4e0b\u8a18\u53c2\u7167\uff09\u3002<\/p>\n<p>&nbsp;<\/p>\n<h4>\u25bc\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u306e\u6b20\u70b9<\/h4>\n<ul>\n<li>\u5206\u6790\u6642\u9593\u304c\u9577\u304f\u306a\u308b\uff06\u30d0\u30c3\u30af\u30d7\u30ec\u30c3\u30b7\u30e3\u30fc\uff08\u80cc\u5727\uff09\u304c\u9ad8\u307e\u308b\n<ul>\n<li>\u30ab\u30e9\u30e0\u306e\u4ed5\u69d8\u3067\u5b9a\u3081\u3066\u3044\u308b\u5727\u529b\u7bc4\u56f2\u3092\u8d85\u3048\u3001\u30ab\u30e9\u30e0\u306e\u5145\u586b\u5264\u3092\u7834\u58ca\u3059\u308b\u304a\u305d\u308c\u304c\u3042\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u6700\u9069\u306a\u5206\u96e2\u6761\u4ef6\u3092\u691c\u8a0e\u3059\u308b\u305f\u3081\u306e\u624b\u9593\u3068\u6642\u9593\u304c\u304b\u304b\u308b<\/li>\n<li>\u9ad8\u5869\u6fc3\u5ea6\u30d0\u30c3\u30d5\u30a1\u30fc\u4e2d\u306b\u30b5\u30f3\u30d7\u30eb\u304c\u3042\u308b\u5834\u5408\u3001\u30ab\u30e9\u30e0\u30d8\u30c3\u30c9\u3067\u6eb6\u96e2\u6db2\u3068\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u304c\u8d77\u3053\u308b\u304a\u305d\u308c\u304c\u3042\u308b\n<ul>\n<li>\u79fb\u52d5\u76f8\u3068\u30b5\u30f3\u30d7\u30eb\u306e\u5869\u6fc3\u5ea6\u304c\u5927\u304d\u304f\u7570\u306a\u308b\u305f\u3081\u3001\u5206\u96e2\u304c\u4f4e\u4e0b\u3059\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h4>\u25bcpH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u306e\u6b20\u70b9<\/h4>\n<ul>\n<li>\u518d\u73fe\u6027\u306e\u3042\u308b\u76f4\u7dda\u306epH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3092\u751f\u6210\u3059\u308b\u3053\u3068\u304c\u96e3\u3057\u3044\u3002\n<ul>\n<li>\u30d7\u30ed\u30b0\u30e9\u30e0\u3055\u308c\u305f\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u306epH\u3092\u5b9f\u6e2c\u3059\u308b\u3068\u3001\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u304c\u66f2\u7dda\u306b\u306a\u3063\u305f\u308a\u3001\u518d\u73fe\u6027\u304c\u60aa\u304f\u306a\u3063\u305f\u308a\u3059\u308b\u5834\u5408\u304c\u3042\u308b\u3002\u305d\u306e\u305f\u3081\u3001\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3092\u5f62\u6210\u3059\u308b\u305f\u3081\u306bpH\u30d0\u30c3\u30d5\u30a1\u30fc\u539f\u6db2\u3092\u8abf\u88fd\u3059\u308b\u3053\u3068\u306f\u6c7a\u3057\u3066\u7c21\u5358\u3067\u306f\u306a\u3044\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>\u307e\u305f\u3001<span style=\"font-size: 18pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=_zsYUjHqiQw\">\u52d5\u753b\u3067\u5206\u304b\u308bpH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u306b\u3088\u308b\u30c1\u30e3\u30fc\u30b8\u30d0\u30ea\u30a2\u30f3\u30c8\u5206\u6790<\/a><\/span>\u3082\u5408\u308f\u305b\u3066\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\uff01<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>mAb\u306e\u30c1\u30e3\u30fc\u30b8\u30d0\u30ea\u30a2\u30f3\u30c8\u5206\u6790\u306b\u304a\u3051\u308b\u30a4\u30aa\u30f3\u4ea4\u63db\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306f\u3001\u5869\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u307e\u305f\u306fpH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3067\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3044\u305a\u308c\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u306b\u3082\u5229\u70b9\u3068\u6b20\u70b9\u304c\u3042\u308a\u307e\u3059\u304c\u3001<strong>\u7279\u306b\u8fd1\u5e74\u306e<\/strong><strong>pH<\/strong><strong>\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u6539\u5584\u306b\u3088\u308a\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u8abf\u88fd\u306e\u554f\u984c\u306f\u307b\u3068\u3093\u3069\u89e3\u6c7a\u3057\u3001\u9ad8\u5206\u96e2\u304b\u3064\u9ad8\u3044\u518d\u73fe\u6027\u3067\u8fc5\u901f\u306b\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u3068\u5206\u96e2\u3092\u884c\u306a\u3048\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002<\/strong>\u305d\u306e\u305f\u3081pH\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u306b\u3088\u308b\u30a2\u30d7\u30ed\u30fc\u30c1\u306f\u5927\u5909\u9b45\u529b\u7684\u306a\u3082\u306e\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u4ee5\u4e0b\u306b\u95a2\u9023\u60c5\u5831\u3092\u63b2\u8f09\u3057\u3066\u304a\u308a\u307e\u3059\u306e\u3067\u3001\u305c\u3072\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h2>\u95a2\u9023\u60c5\u5831<\/h2>\n<p>\u2714<a 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href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/industrial\/pharma-biopharma\/biopharmaceutical-analytical-testing\/intact-protein-analysis-workflows\/charge-variant-analysis.html?CID=Social\">\u30c1\u30e3\u30fc\u30b8\u30d0\u30ea\u30a2\u30f3\u30c8\u5206\u6790\u306b\u3064\u3044\u3066<\/a><\/p>\n<p>\u2714<a href=\"http:\/\/apps.thermoscientific.com\/media\/cmd\/Biopharma_Compendium\/#6?CID=Social\">\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u7279\u6027\u89e3\u6790\u306b\u95a2\u3059\u308b\u60c5\u5831\uff1a\u300eBiopharmaceutical Characterization Application 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