{"id":859,"date":"2018-07-13T00:00:00","date_gmt":"2018-07-13T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/asktaqmanvideo\/"},"modified":"2025-10-15T16:31:23","modified_gmt":"2025-10-15T07:31:23","slug":"asktaqmanvideo","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/asktaqmanvideo\/","title":{"rendered":"Ask TaqMan – \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u3064\u3044\u3066\u306e\u8cea\u554f\u306f\u3042\u308a\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u4f7f\u3044\u59cb\u3081\u306e\u65b9\u3082\u3001\u9577\u5e74\u4f7f\u3063\u3066\u3044\u308b\u65b9\u3082\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3084\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3064\u3044\u3066\u8cea\u554f\u304c\u3042\u308b\u3067\u3057\u3087\u3046\u3002<\/p>\n\n\n\n

\u305d\u3093\u306a\u3042\u306a\u305f\u306e\u8cea\u554f\u3092Ask TaqMan\u30d3\u30c7\u30aa\u3067\u6211\u3005\u30b9\u30bf\u30c3\u30d5\u304c\u7b54\u3048\u307e\u3059\u3002\u7686\u69d8\u3082\u7591\u554f\u306b\u601d\u3046\u3053\u3068\u304c\u3042\u308c\u3070\u3001Asq Taqman\u3067\u8cea\u554f\u3092\u6295\u7a3f\u3057\u3066\u307f\u3066\u306f\u3044\u304b\u304c\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n\n\n\n

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\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u30b9\u30ec\u30c3\u30b7\u30e5\u30db\u30fc\u30eb\u30c9\u3063\u3066\u3069\u3053\u306b\u5f15\u304f\u306e\uff1f<\/strong><\/h2>\n\n\n\n

\uff1c\u30d3\u30c7\u30aa\u306e\u5185\u5bb9\uff1e<\/h4>\n\n\n\n

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\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0<\/strong>PCR<\/strong>\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u3063\u3066\u4f55\uff1f<\/strong><\/h2>\n\n\n\n

\uff1c\u30d3\u30c7\u30aa\u306e\u5185\u5bb9\uff1e<\/h4>\n\n\n\n

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\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u64cd\u4f5c\u3092\u884c\u3046\u524d\u306e\u30c7\u30fc\u30bf\u3067\u306f\u3001\u5404\u30b5\u30f3\u30d7\u30eb\u306e\u51fa\u767a\u70b9\u304c\u3059\u3079\u3066y\u8ef8\u4e0a\u3067\u308f\u305a\u304b\u306b\u7570\u306a\u308b\u305f\u3081\u3001\u30ea\u30cb\u30a2\u30b9\u30b1\u30fc\u30eb\u3067\u306e\u3053\u306e\u66f2\u7dda\u90e8\u5206\u306e\u898b\u6804\u3048\u304c\u3088\u304f\u3042\u308a\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u30ce\u30a4\u30ba\u3092\u5dee\u3057\u5f15\u304f\u3068\u3001\u3059\u3079\u3066\u306e\u30b5\u30f3\u30d7\u30eb\u304c\u30bc\u30ed\u70b9\u304b\u3089\u51fa\u767a\u3057\u3001\u305d\u306e\u7d50\u679c\u7dba\u9e97\u306a\u30c7\u30fc\u30bf\u304c\u5f97\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n

\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306b\u95a2\u3057\u3066\u898f\u6e96\u5316\u3057\u305f\u5f8c\u306e\u5024\u3092\u0394Rn\u3068\u547c\u3073\u307e\u3059\u3002\u5bfe\u6570\u30b9\u30b1\u30fc\u30eb\u306e\u5897\u5e45\u66f2\u7dda\u3092\u3088\u304f\u898b\u308b\u3068\u3001\u898b\u6163\u308c\u305f\u5897\u5e45\u66f2\u7dda\u306ey\u8ef8\u4e0a\u306b\u30d7\u30ed\u30c3\u30c8\u3055\u308c\u3066\u3044\u308b\u306e\u304c\u0394Rn\u3067\u3042\u308b\u3053\u3068\u306b\u6c17\u3065\u304d\u307e\u3059\u3002<\/p>\n\n\n\n

Applied Biosystems\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR<\/a>\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u306f\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u8a2d\u5b9a\u306e\u305f\u3081\u306e\u65b9\u6cd5\u304c2\u7a2e\u985e\u3042\u308a\u307e\u3059\u3002\u30de\u30cb\u30e5\u30a2\u30eb\u30e2\u30fc\u30c9\u3068\u30aa\u30fc\u30c8\u30e2\u30fc\u30c9\u3067\u3059\u3002\u30de\u30cb\u30e5\u30a2\u30eb\u30e2\u30fc\u30c9\u3067\u884c\u3046\u5834\u5408\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u9818\u57df\u306e\u8a2d\u5b9a\u306f\u3001Analysis settings\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u884c\u3044\u307e\u3059\u30021\u3064\u306e\u30a2\u30c3\u30bb\u30a4\u306b\u8a2d\u5b9a\u3057\u305f\u5834\u5408\u306b\u306f\u3001\u305d\u306e\u30a2\u30c3\u30bb\u30a4\u306e\u5168\u3066\u306e\u30a6\u30a7\u30eb\u306b\u95a2\u3057\u3066\u540c\u3058\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u64cd\u4f5c\u304c\u884c\u308f\u308c\u307e\u3059\u3002Advanced settings\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u5404\u30a6\u30a7\u30eb\u306b\u5225\u3005\u306e\u64cd\u4f5c\u3092\u884c\u3046\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002<\/p>\n\n\n\n

\u3055\u3089\u306b\u4fbf\u5229\u306a\u30c7\u30d5\u30a9\u30eb\u30c8\u3067Auto baseline\u8a2d\u5b9a\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u3001\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u304c\u5dee\u3057\u5f15\u304f\u3079\u304d\u30ce\u30a4\u30ba\u3092\u5404\u30a6\u30a7\u30eb\u306b\u95a2\u3057\u3066\u500b\u5225\u306b\u6c7a\u5b9a\u3057\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u3063\u3066\u751f\u6210\u3055\u308c\u308b\u306e\u304c\u3001\u4e00\u822c\u7684\u306b\u306f\u6700\u826f\u306e\u7d50\u679c\u3067\u3059\u3002<\/p>\n\n\n\n

\u3067\u306f\u3001\u306a\u305c\u30de\u30cb\u30e5\u30a2\u30eb\u30e2\u30fc\u30c9\u304c\u5fc5\u8981\u306a\u306e\u3067\u3057\u3087\u3046\uff1f\u5b9f\u306f\u3001\u30aa\u30fc\u30c8\u30e2\u30fc\u30c9\u3067\u306f\u4e0a\u624b\u304f\u3044\u304b\u306a\u3044\u5834\u5408\u304c\u3042\u308b\u306e\u3067\u3059\u3002\u7279\u306bSYBR\u30a2\u30c3\u30bb\u30a4\u3084\u6a19\u6e96\u7684\u3067\u306a\u3044\u5b9f\u9a13\u7cfb\u306e\u5834\u5408\u3067\u3059\u3002\u30aa\u30fc\u30c8\u30e2\u30fc\u30c9\u3067\u826f\u597d\u306a\u7d50\u679c\u304c\u5f97\u3089\u308c\u306a\u3044\u30b1\u30fc\u30b9\u306f\u3001\u5897\u5e45\u66f2\u7dda\u306e\u5f62\u3092\u898b\u308c\u3070\u5206\u304b\u308a\u307e\u3059\u3002\u3082\u3057\u5897\u5e45\u66f2\u7dda\u304c\u901a\u5e38\u3088\u308a\u3082S\u5b57\u578b\u306b\u306a\u3063\u3066\u3044\u308b\u5834\u5408\u3001\u539f\u56e0\u3068\u3057\u3066\u3001\u30aa\u30fc\u30c8\u30e2\u30fc\u30c9\u306b\u3088\u308a\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u304c\u6b63\u3057\u304f\u8a2d\u5b9a\u3055\u308c\u3066\u304a\u3089\u305a\u3001\u30a8\u30f3\u30c9\u30b5\u30a4\u30af\u30eb\u304c\u4f4e\u304f\u306a\u3063\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u3001\u5341\u5206\u306a\u30ce\u30a4\u30ba\u304c\u5dee\u3057\u5f15\u304b\u308c\u305a\u3001\u5897\u5e45\u66f2\u7dda\u306e\u5f62\u304c\u7570\u5e38\u306b\u306a\u308a\u307e\u3059\u3002\u554f\u984c\u3092\u89e3\u6c7a\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u30de\u30cb\u30e5\u30a2\u30eb\u30e2\u30fc\u30c9\u306b\u5207\u308a\u66ff\u3048\u3066\u30b5\u30a4\u30af\u30eb\u6570\u3092\u4e0a\u6607\u3055\u305b\u3001\u5897\u5e45\u66f2\u7dda\u3092\u6b63\u5e38\u306a\u5f62\u306b\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0<\/strong>PCR<\/strong>\u30a2\u30c3\u30bb\u30a4\u30c7\u30b6\u30a4\u30f3\u306e\u30d2\u30f3\u30c8<\/strong><\/h2>\n\n\n\n

\uff1c\u30d3\u30c7\u30aa\u306e\u5185\u5bb9\uff1e<\/h4>\n\n\n\n

Q\uff1a\u3053\u308c\u307e\u3067PCR\u3092\u884c\u3063\u3066\u304d\u305f\u65b9\u304c\u521d\u3081\u3066\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u884c\u3046\u3068\u304d\u4f55\u304b\u7279\u5225\u306a\u3053\u3068\u3092\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/strong><\/p>\n\n\n\n

A\uff1a\u6ce8\u610f\u3059\u308b\u3079\u304d1\u756a\u76ee\u306e\u30dd\u30a4\u30f3\u30c8\u306f\u3001PCR\u7523\u7269\u306e\u9577\u3055\u3067\u3059\u3002PCR\u7523\u7269\u9396\u9577\u304c\u9577\u3059\u304e\u308b\u3068PCR\u52b9\u7387\u304c\u60aa\u304f\u306a\u308b\u305f\u3081\u3001\u53ef\u80fd\u306a\u304b\u304e\u308a\u3001PCR\u7523\u7269\u9396\u9577\u306f150-150bp\u306e\u7bc4\u56f2\u5185\u3067\u8a2d\u8a08\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

2\u756a\u76ee\u306e\u30dd\u30a4\u30f3\u30c8\u306f\u30df\u30b9\u30de\u30c3\u30c1\u3084\u65e2\u77e5\u306eSNP\u3092\u907f\u3051\u308b\u3053\u3068\u3067\u3059\u3002\u5897\u5e45\u306e\u30bf\u30a4\u30df\u30f3\u30b0\u304c\u9045\u308c\u307e\u3059\u3002\u5b9a\u91cf\u5b9f\u9a13\u3067\u306f\u7279\u306b\u91cd\u8981\u3067\u3059\u3002\u3053\u306e\u305f\u3081\u914d\u5217\u306e\u6b63\u78ba\u6027\u304c\u3068\u3066\u3082\u5927\u5207\u3067\u3059\u3002<\/p>\n\n\n\n

3\u756a\u76ee\u306e\u30dd\u30a4\u30f3\u30c8\u306f\u3001\u914d\u5217\u306e\u7279\u7570\u6027\u3067\u3059\u3002\u7279\u306b\u975e\u5e38\u306b\u76f8\u540c\u6027\u304c\u9ad8\u3044\u914d\u5217\u304c\u3042\u308b\u3068\u3001\u975e\u7279\u7570\u7684\u306a\u5897\u5e45\u306e\u5371\u967a\u6027\u304c\u9ad8\u307e\u308b\u306e\u3067\u3001\u5c11\u306a\u304f\u3068\u30821\u672c\u306e\u30aa\u30ea\u30b4\u306b\u306f\u7279\u7570\u6027\u306e\u9ad8\u3044\u9818\u57df\u3092\u542b\u3081\u3066\u8a2d\u8a08\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

\u6700\u5f8c\u306e\u30dd\u30a4\u30f3\u30c8\u306f\u3001\u7e70\u308a\u8fd4\u3057\u914d\u5217\u306a\u3069\u306e\u3001\u914d\u5217\u306e\u7279\u7570\u6027\u304c\u4f4e\u304f\u306a\u308b\u9818\u57df\u3092\u907f\u3051\u308b\u3053\u3068\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u6761\u4ef6\u3092\u3059\u3079\u3066\u6e80\u305f\u3059\u305f\u3081\u306b\u3001\u3054\u81ea\u8eab\u3067\u914d\u5217\u60c5\u5831\u3092\u7cbe\u67fb\u3057\u305f\u5f8c\u3001Primer Express\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3067\u8a2d\u8a08\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3042\u3068\u306f\u30aa\u30ea\u30b4\u5408\u6210\u3059\u308b\u3060\u3051\u3067\u3059\u3002<\/p>\n\n\n\n

Primer Express\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3067\u8a2d\u8a08\u3059\u308b\u3068\u3001\u5171\u901a\u306e\u6761\u4ef6\u3067\u5b9f\u9a13\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u6700\u9069\u5316\u306f\u4e0d\u8981\u3067\u3059\u3002\u307e\u305f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u3067\u306f\u3001\u30c7\u30b6\u30a4\u30f3\u6e08\u307f\u3001\u304a\u3088\u3073\u30ab\u30b9\u30bf\u30e0\u30c7\u30b6\u30a4\u30f3\u306b\u5bfe\u5fdc\u3057\u305f\u3001\u9ad8\u54c1\u8cea\u306eTaqMan\u30a2\u30c3\u30bb\u30a4\u3092\u3054\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u7cbe\u67fb\u6e08\u307f\u306e\u914d\u5217\u3001\u5171\u901a\u306e\u5b9f\u9a13\u6761\u4ef6\u3001100%\u306e\u5897\u5e45\u52b9\u7387 \u3053\u308c\u3089\u5168\u3066\u304c\u304a\u5ba2\u69d8\u306b\u9ad8\u3044\u3054\u6e80\u8db3\u3092\u304a\u5c4a\u3051\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u69d8\u3005\u306a\u751f\u7269\u7a2e\u306b\u5bfe\u5fdc\u3059\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u7528\u306e\u8a2d\u8a08\u6e08\u307f TaqMan\u30a2\u30c3\u30bb\u30a4\u3084\u3001SNP\u30bf\u30a4\u30d4\u30f3\u30b0\u3001microRNA\u3084\u30b3\u30d4\u30fc\u6570\u591a\u578b\u691c\u51fa\u7528\u306e\u30a2\u30c3\u30bb\u30a4\u3082\u3054\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u3082\u3057\u7121\u304f\u3066\u3082\u3054\u5b89\u5fc3\u304f\u3060\u3055\u3044\u3001\u3042\u306a\u305f\u306e\u305f\u3081\u306e\u30a2\u30c3\u30bb\u30a4\u3092\u3054\u7528\u610f\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

Taqman Assay\u306e\u691c\u7d22\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n\n\n\n

\u2191\u4e0a\u8a18\u691c\u7d22\u30c4\u30fc\u30eb\u306e\u30de\u30cb\u30e5\u30a2\u30eb\u306f\u3053\u3061\u3089<\/a><\/strong><\/p>\n\n\n\n

\n

\u521d\u3081\u3066\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30bb\u30df\u30ca\u30fc<\/h2>\n\n\n\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30bb\u30df\u30ca\u30fc\u521d\u5fc3\u8005\u306b\u30d4\u30c3\u30bf\u30ea\u306a\u3001\u521d\u3081\u3066\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30bb\u30df\u30ca\u30fc\u3092\u958b\u50ac\u3057\u307e\u3059\u3002
4\u62e0\u70b9\u3067\u8907\u6570\u56de\u5b9f\u65bd\u3055\u308c\u308b\u7121\u511f\u30bb\u30df\u30ca\u30fc\u3067\u3059\u306e\u3067\u3001\u305c\u3072\u4e0b\u8a18\u30dc\u30bf\u30f3\u3092\u30af\u30ea\u30c3\u30af\u3057\u3001\u304a\u6c17\u8efd\u306b\u3054\u5fdc\u52df\u304f\u3060\u3055\u3044\uff01<\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

\u7121\u511f\u30bb\u30df\u30ca\u30fc\u306e\u8a73\u7d30\u3092\u898b\u308b<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n
\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30cf\u30f3\u30c9\u30d6\u30c3\u30af \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n\n\n\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7406\u8ad6\u3084\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u306e\u8a2d\u8a08\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u77e5\u8b58\u304c\u63b2\u8f09\u3055\u308c\u305f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u59cb\u3081\u305f\u3070\u304b\u308a\u306e\u65b9\u3084\u3053\u308c\u304b\u3089\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n
\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n\n\n\n

\u7570\u5e38\u306aS\u5b57\u72b6\u306e\u5897\u5e45\u66f2\u7dda\u3001NTC\u3067\u306e\u5897\u5e45\u691c\u51fa\u3001\u5897\u5e45\u304c\u898b\u3089\u308c\u306a\u3044\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u5b9f\u9a13\u4e2d\u306b\u56f0\u3063\u305f\u969b\u306e\u624b\u52a9\u3051\u306b\u306a\u308b\u60c5\u5831\u3092\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n
\n

TaqMan Gene Expression\u30a2\u30c3\u30bb\u30a4\u5c0e\u5165\u30ac\u30a4\u30c9 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n\n\n\n

TaqMan\u30a2\u30c3\u30bb\u30a4\u3092\u3053\u308c\u304b\u3089\u4f7f\u3046\u65b9\u3001\u4f7f\u3063\u3066\u3044\u308b\u3051\u3069\u3082\u3063\u3068\u3088\u304f\u77e5\u308a\u305f\u3044\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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TaqMan\u30d3\u30c7\u30aa\u3067\u6211\u3005\u30b9\u30bf\u30c3\u30d5\u304c\u7b54\u3048\u307e\u3059\u3002\u7686\u69d8\u3082\u7591\u554f\u306b\u601d\u3046\u3053\u3068\u304c\u3042\u308c\u3070<\/p>\n","protected":false},"author":988,"featured_media":3632,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[7],"division":[],"class_list":{"0":"post-859","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-qpcr","9":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"\nAsk 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