{"id":8806,"date":"2021-02-12T17:03:21","date_gmt":"2021-02-12T08:03:21","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=8806"},"modified":"2023-12-27T15:26:32","modified_gmt":"2023-12-27T06:26:32","slug":"covid_ngs_gsd_ts_7","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/covid_ngs_gsd_ts_7\/","title":{"rendered":"\u8178\u5185\u7d30\u83cc\u53e2\u3068COVID-19\u306e\u91cd\u75c7\u5ea6\u306e\u95a2\u9023"},"content":{"rendered":"<p>SARS-CoV-2\u306b\u3088\u308b\u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u75c7\uff08COVID-19\uff09\u306f\u308f\u304c\u56fd\u3067\u3082\u5927\u304d\u306a\u8105\u5a01\u3068\u306a\u3063\u3066\u304a\u308a\u3001\u5404\u5730\u3067\u533b\u7642\u4f53\u5236\u306b\u751a\u5927\u306a\u5f71\u97ff\u3092\u53ca\u307c\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u72b6\u6cc1\u306e\u4e2d\u3001\u5e74\u9f62\u3084\u57fa\u790e\u75be\u60a3\u306a\u3069\u500b\u4eba\u304c\u6301\u3064\u4f53\u5185\u306e\u7279\u5fb4\u306b\u3088\u308a\u3001COVID-19\u306e\u91cd\u75c7\u5ea6\u304c\u7570\u306a\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u3066\u304d\u307e\u3057\u305f\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"wp-image-8807 aligncenter\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/02\/20210210_1.jpg\" alt=\"\" width=\"255\" height=\"260\" \/><\/p>\n<p>\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u8178\u5185\u7d30\u83cc\u53e2\u3068COVID-19\u306e\u91cd\u75c7\u5ea6\u306e\u95a2\u9023\u306b\u3064\u3044\u3066\u3001\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<br \/>\n\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<br \/>\n\u30fb\u611f\u67d3\u75c7\u7814\u7a76\u8005<br \/>\n\u30fb\u8178\u5185\u7d30\u83cc\u53e2\u7814\u7a76\u8005<br \/>\n\u30fb\u514d\u75ab\u7814\u7a76\u8005<\/p>\n<p>\u6628\u4eca\u306eSARS-CoV-2\u306b\u3088\u308b\u5371\u6a5f\u306f\u3001\u4e16\u754c\u4e2d\u306e\u4f55\u5341\u5104\u3082\u306e\u4eba\u3005\u306b\u591a\u5927\u306a\u5f71\u97ff\u3092\u4e0e\u3048\u3066\u304a\u308a\u3001\u4e16\u754c\u4e2d\u3067\u52b9\u679c\u7684\u306a\u30ef\u30af\u30c1\u30f3\u306e\u958b\u767a\u304c\u6c42\u3081\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u72b6\u6cc1\u306b\u304a\u3044\u3066\u3001COVID-19\u306e\u91cd\u75c7\u5ea6\u306f\u4eba\u306b\u3088\u3063\u3066\u683c\u5dee\u304c\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u3066\u304d\u3066\u304a\u308a\u3001\u5b89\u5168\u306a\u6cbb\u7642\u6cd5\u306e\u8a55\u4fa1\u3084\u78ba\u7acb\u306e\u305f\u3081\u306b\u3001\u3053\u306e\u9055\u3044\u304c\u4f55\u306b\u8d77\u56e0\u3057\u3066\u3044\u308b\u306e\u304b\u5e83\u304f\u8abf\u3079\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u3053\u306e\u91cd\u75c7\u5ea6\u306e\u9055\u3044\u306b\u95a2\u3057\u3066\u3001\u4e2d\u56fd\u306e\u7814\u7a76\u8005\u304b\u3089COVID-19\u306e\u60a3\u8005\u306e\u514d\u75ab\u72b6\u614b\u306b\u3001\u8178\u5185\u7d30\u83cc\u304c\u91cd\u5927\u306a\u5f71\u97ff\u3092\u4e0e\u3048\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\u3002\u3053\u306e\u5831\u544a\u3067\u306f\u3001COVID-19\u306e\u60a3\u8005\u306b\u304a\u3044\u3066\u3001\u30e9\u30af\u30c8\u30d0\u30c1\u30eb\u30b9\u3084\u4e73\u9178\u83cc\u306a\u3069\u306e\u30d7\u30ed\u30d0\u30a4\u30aa\u30c6\u30a3\u30af\u30b9\u306e\u6e1b\u5c11\u304c\u767a\u751f\u3057\u3001\u8178\u6a5f\u80fd\u306e\u4f4e\u4e0b\u3092\u793a\u3059\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u3001SARS-CoV-2\u306e\u611f\u67d3\u3068\u6804\u990a\u3001\u80c3\u8178\u6a5f\u80fd\u306e\u8a55\u4fa1\u304c\u5fc5\u8981\u3067\u3042\u308b\u3068\u8ff0\u3079\u3066\u3044\u307e\u3059<sup>1<\/sup>\u3002<\/p>\n<p>\u307e\u305f\u4ed6\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u3067\u306f\u3001\u30d2\u30c8\u306e\u8178\u5185\u7d30\u83cc\u53e2\u306e\u7d44\u6210\u3092\u89e3\u6790\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001COVID-19\u306e\u91cd\u75c7\u5ea6\u3092\u4e88\u6e2c\u3067\u304d\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059<sup>2<\/sup>\u3002\u3053\u306e\u7814\u7a76\u3067\u306f\u3001SARS-CoV-2\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u8005\u306e60\uff05\u4ee5\u4e0a\u304c\u4e0b\u75e2\u3001\u60aa\u5fc3\u3001\u5614\u5410\u306a\u3069\u306e\u80c3\u8178\u75c7\u72b6\u3092\u793a\u3057\u3001\u3053\u306e\u30b0\u30eb\u30fc\u30d7\u306e\u60a3\u8005\u304c\u91cd\u75c7\u5ea6\u306e\u9ad8\u3044\u50be\u5411\u306b\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306bSARS-CoV-2\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u800531\u4eba\u306e\u8840\u6db2\u3092\u8abf\u3079\u305f\u3068\u3053\u308d\u3001\u5168\u8eab\u6027\u708e\u75c7\u6642\u306b\u4e0a\u6607\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u514d\u75ab\u56e0\u5b50\u3092\u542b\u3080\u3001\u75be\u60a3\u306e\u91cd\u75c7\u5ea6\u306b\u95a2\u9023\u3059\u308b20\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e0a\u6607\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002 \u3053\u308c\u3089\u306e\u7d50\u679c\u306b\u57fa\u3065\u3044\u3066\u3001\u7814\u7a76\u8005\u3089\u306f\u3001\u75be\u60a3\u306e\u91cd\u75c7\u5ea6\u3092\u4e88\u6e2c\u3067\u304d\u308b\u300c\u30d7\u30ed\u30c6\u30aa\u30df\u30af\u30b9\u30ea\u30b9\u30af\u30b9\u30b3\u30a2\u300d\uff08PRS\uff09\u3092\u4f5c\u6210\u3057\u307e\u3057\u305f\u3002\u6a5f\u68b0\u5b66\u7fd2\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u3092\u4f7f\u7528\u3057\u3066PRS\u3092\u8178\u5185\u7d30\u83cc\u53e2\u306e\u7d44\u6210\u3068\u76f8\u95a2\u3055\u305b\u3001\u8178\u5185\u7d30\u83cc\u53e2\u3068COVID-19\u75be\u60a3\u306e\u91cd\u75c7\u5ea6\u3068\u306e\u95a2\u9023\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u7d50\u679c\u3001Ruminococcus gnavus\u3084Clostridia\u306a\u3069\u3001\u708e\u75c7\u3068\u6b63\u307e\u305f\u306f\u8ca0\u306e\u76f8\u95a2\u95a2\u4fc2\u306b\u3042\u308b\u7279\u5b9a\u306e\u7d30\u83cc\u7a2e\u304c\u691c\u51fa\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p>\u3053\u308c\u3089\u306e\u7814\u7a76\u304b\u3089\u3001COVID-19\u306e\u91cd\u75c7\u5ea6\u3068\u8178\u5185\u7d30\u83cc\u53e2\u306e\u6f5c\u5728\u7684\u306a\u95a2\u9023\u304c\u793a\u5506\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u5225\u306e\u30b0\u30eb\u30fc\u30d7\u306f\u3001COVID-19\u306e\u60a3\u8005\u306b\u5bfe\u3057\u3066\u3001\u30d7\u30ed\u30d0\u30a4\u30aa\u30c6\u30a3\u30af\u30b9\u3092\u30b5\u30d7\u30ea\u30e1\u30f3\u30c8\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\u306b\u306fSARS-CoV-2\u306b\u3064\u3044\u3066\u306e\u3088\u308a\u591a\u304f\u306e\u30c7\u30fc\u30bf\u304c\u5fc5\u8981\u3067\u3042\u308a\u3001\u8178\u5185\u7d30\u83cc\u53e2\u306e\u8abf\u7bc0\u306b\u3088\u308a\u7684\u3092\u7d5e\u3063\u305f\u6cbb\u7642\u30a2\u30d7\u30ed\u30fc\u30c1\u304c\u5fc5\u8981\u306b\u306a\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059<sup>3<\/sup>\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u3053\u308c\u3089\u306e\u95a2\u9023\u3092\u3088\u308a\u8a73\u7d30\u306b\u7814\u7a76\u3057\u3066\u3044\u304f\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>Ion Torrent\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/dna-sequencing\/microbial-sequencing\/microbial-identification-ion-torrent-next-generation-sequencing\/ion-ampliseq-microbiome-health-research-kit.html?CID=csd_cdx_sbu_r04_jp_cp1445_pjt7332_csd00000_0so_blg_op_awa_oc_s00_ngs34_Social_LAB\">Ion AmpliSeq\u2122 Microbiome Health Research Kit<\/a>\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8NGS\u30d9\u30fc\u30b9\u306e\u30a2\u30c3\u30bb\u30a4\u3067\u8178\u5185\u7d30\u83cc\u53e2\u306e\u7814\u7a76\u306b\u6700\u9069\u306a\u30d1\u30cd\u30eb\u306b\u3088\u308a\u3001\u8178\u5185\u7d30\u83cc\u53e2\u306e\u5305\u62ec\u7684\u3067\u30b3\u30b9\u30c8\u30e1\u30ea\u30c3\u30c8\u306e\u9ad8\u3044\u89e3\u6790\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002\u7d30\u83cc\u53e2\u89e3\u6790\u306b\u3088\u304f\u7528\u3044\u3089\u308c\u308b16S rRNA\u907a\u4f1d\u5b50\u306e9\u3064\u306e\u8d85\u53ef\u5909\u9818\u57df\u306e\u3046\u30618\u3064\u3092\u30ab\u30d0\u30fc\u3059\u308b\u30d1\u30cd\u30eb\u3068\u6297\u30a6\u30a4\u30eb\u30b9\u7279\u6027\u3092\u6301\u3064\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u591a\u6570\u306e\u7d30\u83cc\u7a2e\u3092\u306f\u3058\u3081\u3001\u514d\u75ab\u8abf\u7bc0\u306b\u95a2\u9023\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u4e3b\u8981\u306a\u8178\u5185\u5fae\u751f\u7269\u3092\u7a2e\u30ec\u30d9\u30eb\u3067\u540c\u5b9a\u3067\u304d\u308b\u30d1\u30cd\u30eb\u3067\u69cb\u6210\u3055\u308c\u3066\u304a\u308a\u3001\u30b7\u30fc\u30b1\u30f3\u30b5\u4ed8\u5c5e\u306e\u89e3\u6790\u30b5\u30fc\u30d0\u30fc\u3067\u30c7\u30fc\u30bf\u89e3\u6790\u3082\u7c21\u5358\u306b\u5b9f\u65bd\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>Ion Torrent\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/dna-sequencing\/microbial-sequencing\/microbial-identification-ion-torrent-next-generation-sequencing\/viral-typing\/coronavirus-research.html?CID=csd_cdx_sbu_r04_jp_cp1445_pjt7332_csd00000_0so_blg_op_awa_oc_s00_ngs34_Social_LAB\">Ion AmpliSeq\u2122 SARS-CoV-2 Research\u30d1\u30cd\u30eb<\/a>\u306f\u3001SARS-CoV-2\u30a6\u30a4\u30eb\u30b9\u30b2\u30ce\u30e0\u309299%\u4ee5\u4e0a\u30ab\u30d0\u30fc\u3059\u308b\u7279\u7570\u7684\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30b7\u30fc\u30b1\u30f3\u30b5\u4ed8\u5c5e\u306e\u89e3\u6790\u30b5\u30fc\u30d0\u30fc\u3067\u30a6\u30a4\u30eb\u30b9\u30b2\u30ce\u30e0\u306b\u6f5c\u3080\u907a\u4f1d\u5b50\u5909\u7570\u3092\u8fc5\u901f\u306b\u89e3\u6790\u53ef\u80fd\u3067\u3059\u3002\u6700\u65b0\u6a5f\u7a2e\u306eIon Torrent\u2122 Genexus\u2122 Integrated Sequencer\u3092\u4f7f\u7528\u3059\u308c\u3070\u3001\u62bd\u51fa\u3057\u305f\u6838\u9178\u304b\u3089\u30c7\u30fc\u30bf\u89e3\u6790\u307e\u3067\u306e\u30cf\u30f3\u30ba\u30aa\u30f3\u30bf\u30a4\u30e0\u306f\u305f\u3063\u305f10\u5206\u30012\u65e5\u9593\u3067\u30c7\u30fc\u30bf\u304c\u5f97\u3089\u308c\u307e\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30fbCOVID-19\u306e\u91cd\u75c7\u5ea6\u306b\u306f\u8178\u5185\u7d30\u83cc\u53e2\u304c\u95a2\u4fc2\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b<br \/>\n\u30fbSARS-CoV-2\u30a6\u30a4\u30eb\u30b9\u306e\u6a5f\u80fd\u3068\u8178\u5185\u7d30\u83cc\u53e2\u306e\u95a2\u9023\u306f\u3055\u3089\u306b\u7814\u7a76\u304c\u5fc5\u8981\u3067\u3042\u308b<\/p>\n<p>\u30d2\u30c8\u306e\u8178\u5185\u7d30\u83cc\u53e2\u3068\u514d\u75ab\u7cfb\u30fb\u6d88\u5316\u5668\u75be\u60a3\u3084\u304c\u3093\u3068\u306e\u95a2\u9023\u306b\u3054\u8208\u5473\u306e\u3042\u308b\u65b9\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/dna-sequencing\/microbial-sequencing\/microbial-identification-ion-torrent-next-generation-sequencing\/ion-ampliseq-microbiome-health-research-kit.html?CID=csd_cdx_sbu_r04_jp_cp1445_pjt7332_csd00000_0so_blg_op_awa_oc_s00_ngs34_Social_LAB\" target=\"_blank\" rel=\"noopener\">Ion AmpliSeq Microbiome Health Research Kit<\/a>\u3092\u3054\u691c\u8a0e\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>SARS-CoV-2\u30a6\u30a4\u30eb\u30b9\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3084\u682a\u540c\u5b9a\u306b\u3054\u8208\u5473\u306e\u3042\u308b\u65b9\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/dna-sequencing\/microbial-sequencing\/microbial-identification-ion-torrent-next-generation-sequencing\/viral-typing\/coronavirus-research.html?CID=csd_cdx_sbu_r04_jp_cp1445_pjt7332_csd00000_0so_blg_op_awa_oc_s00_ngs34_Social_LAB\" target=\"_blank\" rel=\"noopener\">Ion AmpliSeq SARS-CoV-2 Research\u30d1\u30cd\u30eb<\/a>\u3092\u3054\u691c\u8a0e\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b9 \u30c7\u30fc\u30bf\u89e3\u6790\u30aa\u30f3\u30e9\u30a4\u30f3\u500b\u5225\u76f8\u8ac7<\/h2>\n<p>IonTorrent\u30e6\u30fc\u30b6\u3067\u30c7\u30fc\u30bf\u89e3\u6790\u306b\u95a2\u3059\u308b\u5177\u4f53\u7684\u306a\u8ab2\u984c\u3092\u304a\u6301\u3061\u306e\u65b9\u306b\u306f\u3001\u7121\u6599\u306e\u500b\u5225\u76f8\u8ac7\u306e\u6642\u9593\u3092\u958b\u8a2d\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/seminars\/ngs-data-analysis-online-consultation.html?CID=csd_cdx_sbu_r04_jp_cp1445_pjt7332_csd00000_0so_blg_op_awa_oc_s00_ngs34_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u8a73\u7d30\u3092\u898b\u308b<\/a><\/p>\n<h2>\u53c2\u8003\u6587\u732e<\/h2>\n<p>1. Xu K, Cai H, Shen Y, Ni Q, Chen Y, Hu S, Li J, Wang H, Yu L, Huang H, Qiu Y, Wei G, Fang Q, Zhou J, Sheng J, Liang T, Li L. (2020) Management of corona virus disease-19 (COVID-19): the Zhejiang experience. Zhejiang Da Xue Xue Bao Yi Xue Ban. 49(1):147-157.<\/p>\n<p>2. https:\/\/www.medrxiv.org\/content\/10.1101\/2020.04.22.20076091v1<\/p>\n<p>3. Mak JWY, Chan FKL, Ng SC. (2020) Probiotics and COVID-19: one size does not fit all. Lancet Gastroenterol Hepatol. 5(7):644-645.<\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>SARS-CoV-2\u306b\u3088\u308b\u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u75c7\uff08COVID-19\uff09\u306f\u308f\u304c\u56fd\u3067\u3082\u5927\u304d\u306a\u8105\u5a01\u3068\u306a\u3063\u3066\u304a\u308a\u3001\u5404\u5730\u3067\u533b\u7642\u4f53\u5236\u306b\u751a\u5927\u306a\u5f71\u97ff\u3092\u53ca\u307c\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u72b6\u6cc1\u306e\u4e2d\u3001\u5e74\u9f62\u3084\u57fa\u790e\u75be\u60a3\u306a\u3069\u500b\u4eba\u304c\u6301\u3064\u4f53\u5185\u306e\u7279\u5fb4\u306b\u3088\u308a\u3001CO<\/p>\n","protected":false},"author":975,"featured_media":8807,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[167,165],"division":[],"class_list":{"0":"post-8806","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-covid-19","9":"tag-ngs","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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