{"id":9622,"date":"2021-06-18T15:28:44","date_gmt":"2021-06-18T06:28:44","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=9622"},"modified":"2021-06-22T14:15:03","modified_gmt":"2021-06-22T05:15:03","slug":"incubation_workshop_bid_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/incubation_workshop_bid_ts_1\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u524d\u306e\u8a66\u85ac\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u6642\u9593\u30010\uff5e24\u6642\u9593\u307e\u3067\u3075\u3063\u3066\u307f\u305f"},"content":{"rendered":"<p>2021\u5e741\u6708\uff5e3\u6708\u306b\u958b\u50ac\u3057\u305f\u300c\u3084\u3063\u3066\u307f\u305f\u30b7\u30ea\u30fc\u30ba\u3000\u30a2\u30a4\u30c7\u30a2\u52df\u96c6\u30a4\u30d9\u30f3\u30c8\u300d\u306b\u305f\u304f\u3055\u3093\u306e\u30a2\u30a4\u30c7\u30a2\u3092\u3054\u5fdc\u52df\u3044\u305f\u3060\u304d\u3042\u308a\u304c\u3068\u3046\u3054\u3056\u3044\u307e\u3057\u305f\uff01<br \/>\n\u7686\u3055\u307e\u304b\u3089\u9802\u3044\u305f\u30a2\u30a4\u30c7\u30a2\u306b\u3064\u3044\u3066\u3001\u793e\u5185\u3067\u691c\u8a0e\u3057Blog\u306e\u8a18\u4e8b\u3068\u3057\u3066\u516c\u958b\u3059\u308b\u30c6\u30fc\u30de\u3092\u6c7a\u5b9a\u3057\u307e\u3057\u305f\uff08<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/result_workshop_lsg_ts_1\/?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u300c\u3084\u3063\u3066\u307f\u305f\u300d\u30a2\u30a4\u30c7\u30a2\u767a\u8868\uff01\u5f53\u793e\u30c6\u30af\u30cb\u30ab\u30eb\u30b9\u30bf\u30c3\u30d5\u304c\u3042\u306a\u305f\u306e\u7591\u554f\u3092\u89e3\u6d88\u3057\u307e\u3059<\/a>\uff09\u3002<\/p>\n<p>\u4eca\u56de\u306f1\u3064\u76ee\u306e\u30c6\u30fc\u30de\u306b\u3064\u3044\u3066\u300c\u3084\u3063\u3066\u307f\u305f\u5b9f\u9a13\u300d\u304c\u5b8c\u4e86\u3057\u305f\u306e\u3067\u3001\u305d\u306e\u7d50\u679c\u3092\u516c\u958b\u3057\u307e\u3059\uff01<\/p>\n<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>\u3053\u306e\u4f01\u753b\u3067\u6700\u521d\u306b\u304a\u5fdc\u3048\u3059\u308b\u3053\u3068\u306b\u306a\u3063\u305f\u306e\u306f\u3001\u30cf\u30f3\u30c9\u30eb\u30cd\u30fc\u30e0\u201c\u305b\u3063\u304b\u3061\u3060\u3051\u3069\u5fd8\u308c\u3093\u574a\u3084\u201d\u3055\u3093\u304b\u3089\u3054\u5fdc\u52df\u3044\u305f\u3060\u3044\u305f\u30a2\u30a4\u30c7\u30a2\u3067\u3059\u3002<\/p>\n<p>\u300cInvitrogen\u2122 Lipofectamine\u2122 2000\u3042\u308b\u3044\u306f3000\u3092\u4f7f\u3063\u3066\u306e\u907a\u4f1d\u5b50\u5c0e\u5165\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3002\u88fd\u54c1\u8aac\u660e\u66f8\u3067\u306fDNA\u3068\u8a66\u85ac\u3092\u6df7\u5408\u3057\u306610\uff5e15\u5206\u307b\u3069\u9759\u7f6e\u3059\u308b\u304c\u3001\u6df7\u5408\u3057\u3066\u3059\u3050\u306b\u7d30\u80de\u6dfb\u52a0\u3057\u305f\u5834\u5408\u30681\uff5e2\u6642\u9593\u307b\u3069\u3057\u3066\u7d30\u80de\u306b\u6dfb\u52a0\u3057\u3066\u3082\u767a\u73fe\u306b\u306f\u554f\u984c\u306a\u3044\u306e\u304b\u3092\u8abf\u3079\u3066\u307b\u3057\u3044\u3002\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u6642\u9593\u304c\u3069\u3046\u3057\u3066\u3082\u5f85\u3066\u306a\u3044\u5834\u5408\u3068\u305b\u3063\u304b\u304f\u6df7\u5408\u3057\u3066\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u6642\u9593\u3092\u5f85\u3063\u3066\u3044\u305f\u306e\u306b\u5225\u4f5c\u696d\u306b\u96c6\u4e2d\u3057\u3066\u3059\u3063\u304b\u308a\u5fd8\u308c\u3066\u3057\u307e\u3063\u305f\u5834\u5408\u3092\u60f3\u5b9a\u3057\u3066\u3044\u307e\u3059\u3002\u300d<\/p>\n<p>\u3053\u306e\u30a2\u30a4\u30c7\u30a2\u3001\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8\u30b9\u30bf\u30c3\u30d5\u306e\u9593\u3067\u3082\u300c\u78ba\u304b\u306b\u3069\u3046\u306a\u308b\u306e\u3060\u308d\u3046\uff1f\u88fd\u54c1\u8aac\u660e\u66f8\u306e\u6307\u5b9a\u6642\u9593\u304b\u3089\u305a\u308c\u308b\u3068\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u306f\u4f4e\u4e0b\u3059\u308b\u3060\u308d\u3046\u3051\u3069\u3001\u3069\u306e\u7a0b\u5ea6\u306a\u306e\u3060\u308d\u3046\uff1f\u300d\u3068\u8b70\u8ad6\u304c\u76db\u308a\u4e0a\u304c\u308a\u3001\u5b9f\u969b\u306b\u5b9f\u9a13\u3057\u3066\u307f\u308b\u3053\u3068\u306b\u3057\u307e\u3057\u305f\uff01<\/p>\n<h2>\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n<p>\u6750\u6599\uff1a<br \/>\nHEK293\uff08\u30d2\u30c8\u80ce\u5150\u814e\u81d3\u7531\u6765\u7d30\u80de\u682a\uff09<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/L3000001?SID=srch-hj-L3000001#\/L3000001?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">Invitrogen\u2122 Lipofectamine\u2122 3000 Transfection Reagent<\/a><br \/>\npcDNA 6.2\/EmGFP expression vector\uff081 \u00b5g\/\u00b5L, \u975e\u58f2\u54c1\uff09<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/31985062#\/31985062?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">Gibco\u2122 Opti-MEM\u00ae I Reduced Serum Media<\/a><\/p>\n<p>\u65b9\u6cd5\uff1a<br \/>\n\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u524d\u65e5\u306b24 well\u30d7\u30ec\u30fc\u30c8\u306b1.5 x 10<sup>5<\/sup> cells\/well\u306eHEK293\u3092\u64ad\u7a2e\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u65b9\u6cd5\u306f<a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/lipofectamine3000_protocol.pdf?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">Lipofectamine 3000 \u306e\u88fd\u54c1\u8aac\u660e\u66f8<\/a>\u306b\u5f93\u3063\u3066\u5b9f\u65bd\u3057\u307e\u3057\u305f\uff08Lipofectamine 3000\u306f1.5 \u00b5L\/well\u3067\u4f7f\u7528\uff09\u3002<\/p>\n<p>\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac\u3068DNA\u306e\u6df7\u5408\u306f\u4e00\u5ea6\u306b\u307e\u3068\u3081\u3066\u5b9f\u65bd\u3057\u30010 min \u30fb 5 min \u30fb 10 min \u30fb 15 min \u30fb 30 min \u30fb 1 hr \u30fb 2 hrs \u30fb 4 hrs \u30fb 6 hrs \u30fb 24 hrs\u3001\u305d\u308c\u305e\u308c\u306e\u9577\u3055\u3067\u5ba4\u6e29\u306b\u3066\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3057\u305f\u5f8c\u3001HEK293\u3092\u57f9\u990a\u3057\u3066\u3044\u308b\u30a6\u30a7\u30eb\u306b\u6ef4\u4e0b\u3057\u3066\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\uff08n=2\uff09\u3002\u6e29\u5ea6\u306e\u5f71\u97ff\u3092\u8abf\u3079\u308b\u305f\u3081\u30016 hrs \u306b\u3064\u3044\u3066\u306e\u307f\u8a66\u85ac\u6df7\u5408\u5f8c\u306b4\u2103\u3067\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3057\u305f\u3082\u306e\u3067\u3082\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002\u4eca\u56de\u306e\u5b9f\u9a13\u3067\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac\u3068DNA\u306e\u6df7\u5408\u5f8c\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u6642\u9593\u306e\u3053\u3068\u3092\u3001\u8a66\u85ac\u653e\u7f6e\u6642\u9593\u3068\u547c\u3073\u307e\u3059\u3002<\/p>\n<p>\u203b\u88fd\u54c1\u8aac\u660e\u66f8\u306b\u8a18\u8f09\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u6761\u4ef6\u306f\u3001\u5ba4\u6e29\u306710\uff5e15 min\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9647\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic0_big-2-1024x409.png\" alt=\"\" width=\"760\" height=\"304\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic0_big-2-1024x409.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic0_big-2-300x120.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic0_big-2-768x306.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic0_big-2-1536x613.png 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic0_big-2.png 1669w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<p>\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u306e\u8a55\u4fa1\u65b9\u6cd5\uff1a<br \/>\n\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5f8c\u306e\u57f9\u990a\u6642\u9593\u306f\u3059\u3079\u3066\u306e\u51e6\u7406\u306724 hrs\u306b\u7d71\u4e00\u3057\u3001\u3053\u308c\u3092\u7d4c\u904e\u3057\u305f\u3082\u306e\u304b\u3089<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-analysis\/flow-cytometry\/flow-cytometers\/attune-nxt-flow-cytometer.html?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">Invitrogen\u2122 Attune\u2122 NxT Flow Cytometer<\/a> \u3092\u7528\u3044\u3066GFP\u767a\u73fe\u7d30\u80de\u306e\u5272\u5408\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u3067\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3092\u7b97\u51fa\u3057\u307e\u3057\u305f\u3002<\/p>\n<h2>\u7d50\u679c\u3068\u8003\u5bdf<\/h2>\n<p>\u5b9f\u9a13\u7d50\u679c1\uff1a\u8a66\u85ac\u653e\u7f6e\u6642\u9593\u3054\u3068\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9648\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic1_big-1-1024x762.png\" alt=\"\" width=\"760\" height=\"566\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic1_big-1-1024x762.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic1_big-1-300x223.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic1_big-1-768x571.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic1_big-1.png 1039w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-9649 size-full\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/table1_big-1-e1624333014962.png\" alt=\"\" width=\"600\" height=\"533\" \/><\/p>\n<p>\u307e\u305a\u3001\u8a66\u85ac\u653e\u7f6e\u6642\u9593\u306b\u3064\u3044\u3066\u691c\u8a0e\u3057\u307e\u3057\u305f\u3002\u88fd\u54c1\u8aac\u660e\u66f8\u306b\u5f93\u3063\u305f\u8a66\u85ac\u653e\u7f6e\u6642\u9593\u3067\u3042\u308b10 min\u304a\u3088\u307315 min\u3067\u306f\u3001\u3069\u3061\u3089\u30822\u53cd\u5fa9\u306e\u5e73\u5747\u306770%\u4ee5\u4e0a\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\uff08\u56f31\u3001\u88681\uff09\u3002\u8a66\u85ac\u3068DNA\u3092\u6df7\u5408\u3057\u3066\u3059\u3050\u306b\u7d30\u80de\u306b\u6dfb\u52a0\u3057\u305f0 min\u3067\u306f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c45.6%\u3068\u4f4e\u304f\u3001\u6df7\u5408\u5f8c\u306b5 min\u8a66\u85ac\u653e\u7f6e\u3059\u308b\u3068\u88fd\u54c1\u8aac\u660e\u66f8\u306b\u5f93\u3063\u305f\u5834\u5408\u3068\u307b\u307c\u540c\u7b49\u306b\u307e\u3067\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u9ad8\u304f\u306a\u308a\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u300130 min \u30fb 1 hr \u30fb 2 hrs \u30fb 4 hrs \u30fb 6 hrs 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class=\"aligncenter size-full wp-image-9650\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic2_big.png\" alt=\"\" width=\"604\" height=\"788\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic2_big.png 604w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/pic2_big-230x300.png 230w\" sizes=\"auto, (max-width: 604px) 100vw, 604px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-9651\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/table2_big.png\" alt=\"\" width=\"806\" height=\"317\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/table2_big.png 806w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/table2_big-300x118.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/06\/table2_big-768x302.png 768w\" sizes=\"auto, (max-width: 806px) 100vw, 806px\" \/><\/p>\n<p>\u6b21\u306b\u3001\u8a66\u85ac\u653e\u7f6e\u6e29\u5ea6\u306e\u5f71\u97ff\u3092\u8abf\u3079\u307e\u3057\u305f\u30026 hrs\u653e\u7f6e\u3059\u308b\u6761\u4ef6\u306e\u5834\u5408\u306e\u307f\u3067\u3001\u5ba4\u6e29\u653e\u7f6e\u30684\u2103\u653e\u7f6e\u3092\u6bd4\u8f03\u3057\u305f\u3068\u3053\u308d\u30014\u2103\u306b\u653e\u7f6e\u3057\u305f\u65b9\u304c\u3084\u3084\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u9ad8\u304f\u306a\u308a\u307e\u3057\u305f\uff08\u56f32\u3001\u88682\uff09\u3002<\/p>\n<p>\u8003\u5bdf<\/p>\n<p>\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306e\u539f\u7406\u306f\u6b21\u306e\u3088\u3046\u306b\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>Lipofectamine\u3092\u542b\u3080\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u30d9\u30fc\u30b9\u306e\u8a66\u85ac\u3067\u306f\u3001\u8ca0\u306b\u8377\u96fb\u3057\u305f\u6838\u9178\u3068\u6b63\u306b\u8377\u96fb\u3057\u305f\u5408\u6210\u8102\u8cea\u304c\u9759\u96fb\u76f8\u4e92\u4f5c\u7528\u306b\u3088\u3063\u3066\u81ea\u767a\u7684\u306b\u7e2e\u5408\u3057\u3001\u6838\u9178\u3068\u5408\u6210\u8102\u8cea\u306e\u8907\u5408\u4f53\u304c\u5f62\u6210\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u8907\u5408\u4f53\u304c\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u3092\u4ecb\u3057\u3066\u7d30\u80de\u306b\u53d6\u308a\u8fbc\u307e\u308c\u3001\u5c0e\u5165DNA\u304c\u6838\u306b\u79fb\u884c\u3059\u308b\u3053\u3068\u3067\u907a\u4f1d\u5b50\u767a\u73fe\u304c\u8a98\u5c0e\u3055\u308c\u307e\u3059\u3002\u8a66\u85ac\u653e\u7f6e\u6642\u95930 min\u3067\u3082GFP\u306e\u767a\u73fe\u304c\u8a98\u5c0e\u3055\u308c\u305f\u306e\u306f\u3001\u8a66\u85ac\u3092\u6df7\u5408\u3057\u305f\u77ac\u9593\u304b\u3089\u3042\u308b\u7a0b\u5ea6\u306f\u6838\u9178\u3068\u5408\u6210\u8102\u8cea\u306e\u8907\u5408\u4f53\u304c\u5f62\u6210\u3055\u308c\u305f\u304b\u3089\u3060\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u4eca\u56de\u306e\u5b9f\u9a13\u3067\u306f\u653e\u7f6e\u6642\u959310 min \u3067\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u3082\u3063\u3068\u3082\u9ad8\u304f\u306a\u308a\u3001\u8a66\u85ac\u653e\u7f6e\u6642\u959330 min \u304f\u3089\u3044\u307e\u3067\u306f\u52b9\u7387\u306f\u9855\u8457\u306b\u306f\u4f4e\u4e0b\u3057\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u4eca\u56de\u3054\u5fdc\u52df\u3044\u305f\u3060\u3044\u305f\u201c\u305b\u3063\u304b\u3061\u3060\u3051\u3069\u5fd8\u308c\u3093\u574a\u3084\u201d\u3055\u3093\u306b\u304a\u5fdc\u3048\u3059\u308b\u306a\u3089\u3070\u3001\u3069\u3046\u3057\u3066\u3082\u8a66\u85ac\u653e\u7f6e\u6642\u9593\u3092\u5f85\u3061\u304d\u308c\u306a\u3044\u5834\u5408\u306f\u3067\u304d\u308b\u3060\u305110 min\u306b\u8fd1\u3065\u304f\u3088\u3046\u306b\u9577\u304f\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3057\u3066\u3044\u305f\u3060\u304d\u3001\u653e\u7f6e\u3057\u3066\u3057\u307e\u3063\u305f\u5834\u5408\u306f30 min \u304f\u3089\u3044\u307e\u3067\u306a\u3089\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3078\u306e\u5f71\u97ff\u306f\u5927\u304d\u304f\u306a\u3044\u3001\u3068\u8a00\u3048\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u305f\u3060\u3057\u3001\u4eca\u56de\u8a66\u3057\u305f\u5b9f\u9a13\u7cfb\u3068\u7570\u306a\u308b\u5b9f\u9a13\u7cfb\u3067\u3082\u540c\u69d8\u306e\u7d50\u679c\u304c\u5f97\u3089\u308c\u308b\u304b\u3069\u3046\u304b\u306f\u4e0d\u660e\u3067\u3059\u306e\u3067\u3001\u305d\u306e\u70b9\u306b\u306f\u6ce8\u610f\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<p>\u307e\u305f\u3001\u653e\u7f6e\u6e29\u5ea6\u306b\u3064\u3044\u3066\u306f6 hrs\u3068\u3044\u3046\u9577\u6642\u9593\u306e\u653e\u7f6e\u3067\u306f4\u2103\u306e\u65b9\u304c\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u9ad8\u304f\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u3053\u3068\u306f\u30014\u2103\u306e\u65b9\u304c\u6838\u9178\u3068\u5408\u6210\u8102\u8cea\u306e\u8907\u5408\u4f53\u306e\u5b89\u5b9a\u6027\u304c\u3084\u3084\u9ad8\u304f\u306a\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u88fd\u54c1\u8aac\u660e\u66f8\u306b\u5f93\u3063\u305f10\uff5e15 min \u306e\u8a66\u85ac\u653e\u7f6e\u6642\u9593\u306e\u5834\u5408\u3067\u306f\u3001\u6e29\u5ea6\u304c\u4f4e\u3044\u3068\u8907\u5408\u4f53\u5f62\u6210\u306e\u52b9\u7387\u3092\u4f4e\u4e0b\u3055\u305b\u3066\u3057\u307e\u3046\u3088\u3046\u306a\u3053\u3068\u3082\u3042\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u3084\u306f\u308a\u3001\u88fd\u54c1\u8aac\u660e\u66f8\u306b\u5f93\u3044\u3001\u8a66\u85ac\u3068DNA\u3092\u6df7\u5408\u3057\u305f\u5f8c\u306f\u3001\u5ba4\u6e29\u306710\uff5e15 min \u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3059\u308b\u306e\u304c\u4e00\u756a\u306e\u304a\u3059\u3059\u3081\u3067\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u3044\u304b\u304c\u3067\u3057\u305f\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n<p>\u4eca\u56de\u306e\u5b9f\u9a13\u3067\u306f\u3001\u52b9\u7387\u306f\u4f4e\u3044\u3067\u3059\u304c\u8a66\u85ac\u653e\u7f6e\u6642\u95930 min\u3067\u3082\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3067\u304d\u308b\u3053\u3068\u306b\u9a5a\u304d\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u63a8\u5968\u306e\u8a66\u85ac\u653e\u7f6e\u6642\u9593\uff0810\uff5e15 min\uff09\u3088\u308a\u5c11\u3057\u9577\u304f\u306a\u308b\u304f\u3089\u3044\u3067\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u306b\u5927\u304d\u306a\u5f71\u97ff\u306f\u306a\u3044\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u306f\u3001\u8a66\u85ac\u3068DNA\u306e\u6df7\u5408\u5f8c\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u6642\u9593\u3060\u3051\u3067\u306a\u304f\u3001\u7d30\u80de\u30bf\u30a4\u30d7\u3084\u305d\u306e\u72b6\u614b\u3001\u4f7f\u7528\u57f9\u5730\u3084\u5c0e\u5165\u3059\u308b\u5206\u5b50\u30bf\u30a4\u30d7\u306a\u3069\u3055\u307e\u3056\u307e\u306a\u8981\u56e0\u306b\u5f71\u97ff\u3055\u308c\u307e\u3059\uff08\u8a73\u7d30\u306f\uff1a<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/factors-influencing-transfection-efficiency\/?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u6210\u529f\u306e\u79d8\u8a23\u3092\u516c\u958b\uff01\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u52b9\u7387\u306b\u5f71\u97ff\u3059\u308b8\u3064\u306e\u8981\u7d20<\/a>\uff09\u3002\u4eca\u56de\u306e\u7d50\u679c\u306f\u3001\u3042\u304f\u307e\u3067\u4eca\u56de\u306e\u6761\u4ef6\u306b\u9650\u5b9a\u3055\u308c\u308b\u3082\u306e\u3067\u3001\u7686\u3055\u307e\u306e\u5b9f\u9a13\u7cfb\u3067\u306f\u5fc5\u305a\u3057\u3082\u7d50\u679c\u304c\u5408\u81f4\u3057\u306a\u3044\u304b\u3082\u3057\u308c\u306a\u3044\u3053\u3068\u3092\u3054\u627f\u77e5\u304a\u304d\u304f\u3060\u3055\u3044\u3002\u305c\u3072\u3001\u88fd\u54c1\u8aac\u660e\u66f8\u306b\u5f93\u3063\u3066\u5b9f\u9a13\u3092\u5b9f\u65bd\u3057\u3066\u3044\u305f\u3060\u3051\u308c\u3070\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<p>\u4eca\u56de\u306e\u5b9f\u9a13\u306f\u30012021\u5e741\u6708\uff5e3\u6708\u306b\u958b\u50ac\u3057\u305f\u300c\u3084\u3063\u3066\u307f\u305f\u30b7\u30ea\u30fc\u30ba\u3000\u30a2\u30a4\u30c7\u30a2\u52df\u96c6\u30a4\u30d9\u30f3\u30c8\u300d\u306b\u3054\u5fdc\u52df\u3044\u305f\u3060\u3044\u305f\u30a2\u30a4\u30c7\u30a2\u306b\u304a\u5fdc\u3048\u3059\u308b\u304b\u305f\u3061\u3067\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002<br \/>\n\u201c\u305b\u3063\u304b\u3061\u3060\u3051\u3069\u5fd8\u308c\u3093\u574a\u3084\u201d\u3055\u3093\u3001\u30a2\u30a4\u30c7\u30a2\u3092\u3054\u5fdc\u52df\u3044\u305f\u3060\u304d\u3042\u308a\u304c\u3068\u3046\u3054\u3056\u3044\u307e\u3057\u305f\uff01<\/p>\n<p>\u73fe\u5728\u3001Blog \u8a18\u4e8b\u3068\u3057\u3066\u516c\u958b\u304c\u6c7a\u5b9a\u3057\u3066\u3044\u308b2\u3064\u76ee\u306e\u30c6\u30fc\u30de\u306b\u3064\u3044\u3066\uff08\u300c<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/result_workshop_lsg_ts_1\/?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3084\u3063\u3066\u307f\u305f\u300d\u30a2\u30a4\u30c7\u30a2\u767a\u8868\u8a18\u4e8b\u3078<\/a>\uff09\u3001\u300c\u3084\u3063\u3066\u307f\u305f\u5b9f\u9a13\u300d\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3059\u306e\u3067\u3001\u6b21\u306e\u516c\u958b\u3092\u697d\u3057\u307f\u306b\u3057\u3066\u3044\u3066\u304f\u3060\u3055\u3044\uff01<\/p>\n<h3>\u5404\u7a2e\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u3092\u958b\u50ac\u4e2d\u3067\u3059<\/h3>\n<p>RNA\u62bd\u51fa\u3084\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3001\u4ed6\u306b\u3082\u7d30\u80de\u57f9\u990a\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306a\u3069\u3001\u5b9f\u969b\u306b\u5b9f\u9a13\uff08\u5b9f\u7fd2\uff09\u3092\u884c\u3044\u3064\u3064\u5b66\u3093\u3067\u3044\u305f\u3060\u3051\u307e\u3059\u3002\u305d\u306e\u4e2d\u3067\u4eca\u56de\u306e\u3088\u3046\u306a\u5b9f\u9a13\u7d50\u679c\u3082\u3054\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u65b0\u3057\u3044\u5b9f\u9a13\u3092\u59cb\u3081\u3089\u308c\u308b\u65b9\u3001\u3088\u308a\u7406\u89e3\u3092\u6df1\u3081\u305f\u3044\u65b9\u306f\u305c\u3072\u3054\u53c2\u52a0\u304f\u3060\u3055\u3044\uff01<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/lifescience-training.html?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u8a73\u7d30\u3092\u898b\u308b<\/a><\/p>\n<p>\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u95a2\u9023\u3059\u308b\u3001\u904e\u53bb\u306e Blog<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-of-transfection\/?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u5b89\u5b9a\uff1f\u305d\u308c\u3068\u3082\u4e00\u904e\u6027\uff1f \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u306a\u3089\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u57fa\u790e\u77e5\u8b58<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-delivery-technologies\/?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u5fb9\u5e95\u7db2\u7f85\uff01\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u304a\u3051\u308b\u5316\u5b66\u7684\u624b\u6cd5\u3001\u751f\u7269\u5b66\u7684\u624b\u6cd5\u3001\u7269\u7406\u7684\u624b\u6cd5\u307e\u3068\u3081<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/factors-influencing-transfection-efficiency\/?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u6210\u529f\u306e\u79d8\u8a23\u3092\u516c\u958b\uff01\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u52b9\u7387\u306b\u5f71\u97ff\u3059\u308b8\u3064\u306e\u8981\u7d20<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/guidelines-and-optimizing-for-plasmid-dna-transfection\/?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30d7\u30e9\u30b9\u30df\u30c9DNA\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u305f\u3081\u306e\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u3068\u6700\u9069\u5316\u65b9\u6cd5<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/guidelines-and-optimizing-for-rna-transfection\/?cid=bid_clb_tfx_r04_jp_cp1433_pjt7389_bid00000_0so_blg_op_awa_og_s00_nap5_Social_LAB\" target=\"_blank\" rel=\"noopener\">RNA\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u305f\u3081\u306e\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u3068\u6700\u9069\u5316\u65b9\u6cd5<\/a><\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>2021\u5e741\u6708\uff5e3\u6708\u306b\u958b\u50ac\u3057\u305f\u300c\u3084\u3063\u3066\u307f\u305f\u30b7\u30ea\u30fc\u30ba\u3000\u30a2\u30a4\u30c7\u30a2\u52df\u96c6\u30a4\u30d9\u30f3\u30c8\u300d\u306b\u305f\u304f\u3055\u3093\u306e\u30a2\u30a4\u30c7\u30a2\u3092\u3054\u5fdc\u52df\u3044\u305f\u3060\u304d\u3042\u308a\u304c\u3068\u3046\u3054\u3056\u3044\u307e\u3057\u305f\uff01 \u7686\u3055\u307e\u304b\u3089\u9802\u3044\u305f\u30a2\u30a4\u30c7\u30a2\u306b\u3064\u3044\u3066\u3001\u793e\u5185\u3067\u691c\u8a0e\u3057Blog\u306e\u8a18\u4e8b\u3068\u3057\u3066\u516c\u958b\u3059\u308b\u30c6\u30fc\u30de\u3092\u6c7a<\/p>\n","protected":false},"author":975,"featured_media":9634,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[67,28],"division":[],"class_list":{"0":"post-9622","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-transfection","9":"tag-experiment","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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