{"id":969,"date":"2019-01-11T00:00:00","date_gmt":"2019-01-11T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cancer8\/"},"modified":"2025-06-19T00:47:23","modified_gmt":"2025-06-18T15:47:23","slug":"cancer8","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cancer8\/","title":{"rendered":"\u3010\u3044\u307e\u3055\u3089\u805e\u3051\u306a\u3044\u304c\u3093\u306e\u57fa\u790e 8\u3011PI3K\/Akt\/mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u3068\u306f\uff1f"},"content":{"rendered":"\n<p>\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb3-\u30ad\u30ca\u30fc\u30bc\/\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bcB\/\u54fa\u4e73\u985e\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\uff08PI3K\/Akt\/mTOR\uff09\u7d4c\u8def\u306f\u3001\u5e83\u7bc4\u306a\u6b63\u5e38\u7d30\u80de\u6a5f\u80fd\u306b\u5fc5\u8981\u3068\u3055\u308c\u308b\u4e3b\u8981\u306a\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30ce\u30fc\u30c9\u3067\u3059\u3002\u3053\u306e\u7d4c\u8def\u306e\u5909\u5316\u304c\u816b\u760d\u5f62\u6210\u306e\u4e00\u56e0\u3067\u3042\u308b\u3053\u3068\u306f\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u306a\u305c\u306a\u3089\u3001\u3053\u306e\u7d4c\u8def\u306f\u3001\u5927\u90e8\u5206\u306e\u304c\u3093\u306e\u30db\u30fc\u30eb\u30de\u30fc\u30af\uff08\u4ee3\u8b1d\u3001\u6210\u9577\u304a\u3088\u3073\u5897\u6b96\u3001\u306a\u3089\u3073\u306b\u8ee2\u79fb\uff09\u306e\u5236\u5fa1\u306b\u6975\u3081\u3066\u91cd\u8981\u3067\u3001\u816b\u760d\u306e\u8840\u7ba1\u65b0\u751f\u3084\u304c\u3093\u95a2\u9023\u708e\u75c7\u306a\u3069\u306e\u816b\u760d\u4fc3\u9032\u30d7\u30ed\u30bb\u30b9\u306b\u3082\u95a2\u4e0e\u3057\u3066\u3044\u308b\u304b\u3089\u3067\u3059\u3002PI3K\/Akt \/mTOR\u7d4c\u8def\u306f\u3001\u3053\u306e\u7d4c\u8def\u306e\u3044\u304f\u3064\u304b\u306e\u30e1\u30c7\u30a3\u30a8\u30fc\u30bf\u30fc\u306b\u304a\u3044\u3066\u3055\u307e\u3056\u307e\u306a\u767a\u304c\u3093\u6027\u5909\u7570\u304a\u3088\u3073\u816b\u760d\u6291\u5236\u6a5f\u80fd\u55aa\u5931\u306b\u3064\u306a\u304c\u308b\u5909\u7570\u304c\u540c\u5b9a\u3055\u308c\u305f\u3053\u3068\u304b\u3089\u3001\u5fb9\u5e95\u7684\u306b\u63a2\u7a76\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u5b9f\u969b\u3001\u304c\u3093\u30b2\u30ce\u30e0\u7814\u7a76\u3067\u306f\u3001\u30d2\u30c8\u304c\u3093\u3067\u306f\u3053\u306e\u7d4c\u8def\u306b\u304a\u3044\u3066\u6700\u3082\u983b\u7e41\u306b\u5909\u5316\u304c\u751f\u3058\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>PI3K\/Akt \/mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3092\u5236\u5fa1\u3059\u308b\u7d30\u80de\u6a5f\u69cb\u304a\u3088\u3073\u5206\u5b50\u6a5f\u69cb\u306b\u95a2\u3059\u308b\u8c4a\u5bcc\u306a\u60c5\u5831\u306f\u3001\u7d4c\u8def\u306e\u963b\u5bb3\u5264\u306e\u958b\u767a\u3092\u30b5\u30dd\u30fc\u30c8\u3057\u3001\u5206\u5b50\u6a19\u7684\u6297\u304c\u3093\u5264\u306e\u5229\u7528\u53ef\u80fd\u6027\u306e\u5897\u52a0\u306b\u5bc4\u4e0e\u3057\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-pi3k-akt-mtor\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30ce\u30fc\u30c9\">PI3K\/Akt\/mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30ce\u30fc\u30c9<\/h2>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u30d7\u30ed\u30c8\u30bf\u30a4\u30d7\u306e\u751f\u5b58\u7d4c\u8def\">\u30d7\u30ed\u30c8\u30bf\u30a4\u30d7\u306e\u751f\u5b58\u7d4c\u8def<\/h4>\n\n\n\n<p>\u6b63\u5e38\u306a\u30db\u30e1\u30aa\u30b9\u30bf\u30b7\u30b9\u72b6\u614b\u4e0b\u3067\u7d30\u80de\u6a5f\u80fd\u306e\u3055\u307e\u3056\u307e\u306a\u5074\u9762\u3092\u30b5\u30dd\u30fc\u30c8\u3057\u3001\u7d30\u80de\u751f\u5b58\u306b\u5bc4\u4e0e\u3059\u308b\u7d30\u80de\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306e\u5909\u5316\u306f\u3001\u816b\u760d\u5f62\u6210\u306e\u9032\u884c\u306e\u4e00\u56e0\u3068\u3055\u308c\u307e\u3059&nbsp;<sup>*1,2<\/sup>\u3002&nbsp;\u816b\u760d\u7d30\u80de\u751f\u7269\u5b66\u306b\u304a\u3044\u3066\u3001\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb3-\u30ad\u30ca\u30fc\u30bc\/\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bcB\/\u54fa\u4e73\u985e\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\uff08PI3K\/Akt\/mTOR\uff09\u30ab\u30b9\u30b1\u30fc\u30c9\u304c\u4e2d\u5fc3\u7684\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3068\u3044\u3046\u4eee\u5b9a\u306e\u3082\u3068\u3001\u3053\u306e\u57fa\u672c\u7684\u306a\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306b\u3064\u3044\u3066\u660e\u3089\u304b\u306b\u3059\u308b\u305f\u3081\u306b\u6ce8\u529b\u3055\u308c\u3066\u304d\u307e\u3057\u305f\uff08\u56f33.1\uff09\u3002\u591a\u6570\u306e\u7814\u7a76\u306b\u304a\u3044\u3066\u3001PI3K\/Akt\/mTOR\u95a2\u9023\u907a\u4f1d\u5b50\u306b\u751f\u3058\u305f\u4f53\u7d30\u80de\u5909\u7570\u306b\u3088\u3063\u3066\u3055\u307e\u3056\u307e\u306a\u30bf\u30a4\u30d7\u306e\u304c\u3093\u7d4c\u8def\u306e\u6052\u5e38\u7684\u6d3b\u6027\u5316\u304c\u8a98\u5c0e\u3055\u308c\u3001\u816b\u760d\u7d30\u80de\u5897\u6b96\u3001\u6210\u9577\u3001\u5206\u5316\u3001\u4ee3\u8b1d\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3001\u304a\u3088\u3073\u816b\u760d\u7d30\u80de\u751f\u5b58\u3092\u30b5\u30dd\u30fc\u30c8\u3059\u308b\u4ed6\u306e\u6a5f\u80fd\u306e\u8abf\u7bc0\u4e0d\u5168\u304c\u5f15\u304d\u8d77\u3053\u3055\u308c\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u793a\u3055\u308c\u3066\u3044\u307e\u3059<sup>*3<\/sup>\u3002<\/p>\n\n\n\n<p>PI3K\/Akt\/mTOR\u7d4c\u8def\u306b\u5f71\u97ff\u3059\u308b\u751f\u6b96\u7d30\u80de\u7cfb\u5217\u306e\u5909\u7570\u306f\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u6027\u304c\u3093\u306b\u95a2\u4e0e\u3057\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u816b\u760d\u6291\u5236\u56e0\u5b50\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u30c6\u30f3\u30b7\u30f3\u30db\u30e2\u30ed\u30b0\uff08PTEN\uff09\u306e\u55aa\u5931\u306f\u3001\u30ab\u30a6\u30c7\u30f3\u75c7\u5019\u7fa4\uff08\u591a\u767a\u6027\u904e\u8aa4\u816b\u75c7\u5019\u7fa4\uff09\u3092\u767a\u75c7\u3055\u305b\u307e\u3059\u3002\u3053\u306e\u5e38\u67d3\u8272\u4f53\u512a\u6027\u75be\u60a3\u306f\u3001\u76ae\u819a\u304a\u3088\u3073\u7c98\u819c\u306e\u826f\u6027\u75c5\u5909\uff08\u904e\u8aa4\u816b\uff09\u306e\u5b58\u5728\u3092\u7279\u5fb4\u3068\u3057\u3001\u4e73\u623f\u3001\u7532\u72b6\u817a\u3001\u304a\u3088\u3073\u4ed6\u306e\u5668\u5b98\u306e\u304c\u3093\u306e\u767a\u751f\u3092\u5897\u52a0\u3055\u305b\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059<sup>*4<\/sup>\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"316\" height=\"161\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PTEN-Mouse-Monoclonal-Antibody-1B8.jpg\" alt=\"PTEN Mouse Monoclonal Antibody (1B8) \" class=\"wp-image-20989\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PTEN-Mouse-Monoclonal-Antibody-1B8.jpg 316w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PTEN-Mouse-Monoclonal-Antibody-1B8-300x153.jpg 300w\" sizes=\"auto, (max-width: 316px) 100vw, 316px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>PTEN Mouse Monoclonal Antibody (1B8)<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">\u6297PTEN\u6297\u4f53 (\u88fd\u54c1\u756a\u53f7 MA5-15560) (\u7dd1\u8272) \u304a\u3088\u3073DRAQ5\u2122 fluorescent DNA Dye (\u9752\u8272) \u3092\u4f7f\u7528\u3057\u305f\u3001HeLa (\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093) \u7d30\u80de (\u5de6) \u304a\u3088\u3073HepG2 (\u4e0d\u6b7b\u5316\u30d2\u30c8\u809d\u7d30\u80de\u304c\u3093) \u7d30\u80de (\u53f3) \u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306fDyLight\u2122 554-Phalloidin\u3067\u6a19\u8b58 (\u8d64\u8272)\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"282\" height=\"170\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PTEN-Rabbit-Polyclonal-Antibody.jpg\" alt=\"PTEN Rabbit Polyclonal Antibody\" class=\"wp-image-20990\" style=\"width:410px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>PTEN Rabbit Polyclonal Antibody<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">(A) \u6297PTEN\u6297\u4f53 (\u88fd\u54c1\u756a\u53f7 PA5-27304) (\u7dd1\u8272) \u3092\u4f7f\u7528\u3057\u305f\u3001HeLa (\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093) \u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002\u03b1\u30c1\u30e5\u30fc\u30d6\u30ea\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3082\u6a19\u8b58 (\u8d64\u8272)\u3002 (B) \u6297PTEN\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3092\u30d7\u30ed\u30fc\u30d6\u3068\u3057\u3066\u4f7f\u7528\u3057\u305f\u3001NIH\/3T3 (\u4e0d\u6b7b\u5316\u30de\u30a6\u30b9\u80da\u7dda\u7dad\u82bd\u7d30\u80de) \u306e\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"374\" height=\"259\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_Phospho-PTEN-pSer370-Rabbit-Polyclonal-Antibody.jpg\" alt=\"Phospho-PTEN pSer370 Rabbit Polyclonal Antibody\" class=\"wp-image-20991\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_Phospho-PTEN-pSer370-Rabbit-Polyclonal-Antibody.jpg 374w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_Phospho-PTEN-pSer370-Rabbit-Polyclonal-Antibody-300x208.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_Phospho-PTEN-pSer370-Rabbit-Polyclonal-Antibody-96x65.jpg 96w\" sizes=\"auto, (max-width: 374px) 100vw, 374px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>Phospho-PTEN pSer370 Rabbit Polyclonal Antibody<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">(A) 3T3-L1 (\u30de\u30a6\u30b9\u8102\u80aa) \u7d30\u80de\u62bd\u51fa\u7269\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\u306b\u304a\u3051\u308b\u6297\u4f53\u30da\u30d7\u30c1\u30c9\u7af6\u5408\u8a66\u9a13\u304a\u3088\u3073\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u51e6\u7406\u306e\u8a55\u4fa1\u3002\u30e1\u30f3\u30d6\u30ec\u30f3\u306f\u3001\u672a\u51e6\u7406 (\u30ec\u30fc\u30f31-4) \u307e\u305f\u306f\u03bb\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u51e6\u7406 (\u30ec\u30fc\u30f35) \u3092\u884c\u3044\u3001\u6297\u30ea\u30f3\u9178\u5316PTEN pSer370\u6297\u4f53 (\u88fd\u54c1\u756a\u53f7 44-1060G) \u3068\u3068\u3082\u306b\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3002\u672c\u30c7\u30fc\u30bf\u306f\u3001PTEN\u306b\u5bfe\u5fdc\u3059\u308b\u30ea\u30f3\u30da\u30d7\u30c1\u30c9\u306e\u307f\u304c\u6297\u4f53\u30b7\u30b0\u30ca\u30eb\u3092\u30d6\u30ed\u30c3\u30af\u3057\u3001\u6297\u4f53\u306b\u5bfe\u3057\u7279\u7570\u6027\u3092\u793a\u3059\u3053\u3068\u3092\u8868\u793a\u3057\u3066\u3044\u307e\u3059\u3002<br>(B) \u6297\u30ea\u30f3\u9178\u5316PTEN pSer370\u6297\u4f53\u3092\u4f7f\u7528\u3057\u305f\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3057\u305f\u30d2\u30c8\u4e73\u304c\u3093 (\u53f3) \u3068\u4e00\u6b21\u6297\u4f53\u4e0d\u4f7f\u7528\u306e\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb (\u5de6) \u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u306b\u304a\u3051\u308b\u6bd4\u8f03\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb3-\u30ad\u30ca\u30fc\u30bc-pi3k\">\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb3-\u30ad\u30ca\u30fc\u30bc\uff08PI3K\uff09<\/h2>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-pi3k\u306e\u6a5f\u80fd\u304a\u3088\u3073\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\">PI3K\u306e\u6a5f\u80fd\u304a\u3088\u3073\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054<\/h4>\n\n\n\n<p>\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb3-\u30ad\u30ca\u30fc\u30bc\uff08PI3K\uff09\u306f\u3001\u7d30\u80de\u819c\u306e\u69cb\u6210\u8981\u7d20\u3067\u30db\u30b9\u30db\u30a4\u30ce\u30b7\u30c1\u30c9\u306e\u4ee3\u8b1d\u524d\u99c6\u4f53\u3067\u3042\u308b\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb\u306e3\u2019-\u6c34\u9178\u57fa\u3092\u30ea\u30f3\u9178\u5316\u3059\u308b\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3001\u5e83\u7bc4\u306a\u7d30\u80de\u6d3b\u6027\u3092\u8abf\u7bc0\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u7d30\u80de\u751f\u5b58\u304a\u3088\u3073\u7d30\u80de\u6b7b\u3092\u5236\u5fa1\u3059\u308b\u751f\u7269\u6d3b\u6027\u8102\u8cea\u5206\u5b50\u3067\u3059\uff08\u56f33.2\uff09<sup>*5,6<\/sup> \u3002PI3K\u306f\u3001\u7d30\u80de\u6a5f\u80fd\u306e\u5236\u5fa1\u306b\u304a\u3044\u3066\u5e45\u5e83\u3044\u5f79\u5272\u3092\u6301\u3064\u4ed6\u3001\u30ea\u30f3\u30d1\u7403\u306e\u6d3b\u6027\u5316\u304a\u3088\u3073\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306b\u95a2\u4e0e\u3057\u3066\u3044\u307e\u3059<sup>*7,8&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"405\" height=\"194\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/e94842e5a45450159acd3ad3d69e61e4-e1748266609352.jpg\" alt=\"\u56f33.2. \u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb (PI) \u306e\u5316\u5b66\u69cb\u9020\" class=\"wp-image-21007\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/e94842e5a45450159acd3ad3d69e61e4-e1748266609352.jpg 405w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/e94842e5a45450159acd3ad3d69e61e4-e1748266609352-300x144.jpg 300w\" sizes=\"auto, (max-width: 405px) 100vw, 405px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.2. \u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb (PI) \u306e\u5316\u5b66\u69cb\u9020<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">\u30db\u30b9\u30db\u30b0\u30ea\u30bb\u30ea\u30c9\u306e\u4e2d\u5fc3\u306f\u3001\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30f3\u9178\u90e8\u5206\u304b\u3089\u69cb\u6210\u3055\u308c\u3001\u74b0\u5f0f\u30a2\u30eb\u30b3\u30fc\u30eb\u306e\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb\u3068\u7d50\u5408\u3057\u3001\u30ea\u30f3\u9178\u30a8\u30b9\u30c6\u30eb\u7d50\u5408\u3092\u5f62\u6210\u3057\u3066\u3044\u307e\u3059\u3002PI\u306eC1\u304a\u3088\u3073C2\u306e\u6c34\u9178\u57fa\u306f\u3001R1\u304a\u3088\u3073R2\u306e\u4f4d\u7f6e\u30672\u3064\u306e\u8102\u80aa\u9178\u2212\u901a\u5e38\u3001\u30b9\u30c6\u30a2\u30ea\u30f3\u9178\u304a\u3088\u3073\u30a2\u30e9\u30ad\u30c9\u30f3\u9178\u2212\u306e\u30ab\u30eb\u30dc\u30ad\u30b7\u30eb\u57fa\u306b\u7d50\u5408\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p>PI3K\u306f\u3001\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u3001\u30a4\u30f3\u30c6\u30b0\u30ea\u30f3\u3001B\u7d30\u80de\u53d7\u5bb9\u4f53\uff08BCR\uff09\u6d3b\u6027\u5316\u3001\u3042\u308b\u3044\u306fG\u30bf\u30f3\u30d1\u30af\u8cea\u5171\u5f79\u53d7\u5bb9\u4f53\uff08GPCR\uff09\u30ea\u30ac\u30f3\u30c9\u306a\u3069\u306e\u8907\u6570\u306e\u4e0a\u6d41\u30b7\u30b0\u30ca\u30eb\u3092\u4ecb\u3057\u3066\u6d3b\u6027\u5316\u3055\u308c\u307e\u3059\u3002PI3K\u304c\u30ea\u30ac\u30f3\u30c9\u306b\u7d50\u5408\u3059\u308b\u3068\u3001\u53d7\u5bb9\u4f53\u30c1\u30ed\u30b7\u30f3\u30ad\u30ca\u30fc\u30bc\uff08RTK\uff09-\u304a\u3088\u3073\uff08GPCR\uff09-\u8a98\u5c0e\u6027\u306ePI3K\u6d3b\u6027\u5316\u304c\u7d30\u80de\u819c\u3067\u751f\u3058\u307e\u3059\u3002\u6d3b\u6027\u5316\u3055\u308c\u305fPI3K\u306f\u3001\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb-4,5- \u4e8c\u30ea\u30f3\u9178\uff08PtdIns[4,5]P2\u3001\u307e\u305f\u306fPIP2\uff09\u3092\u30ea\u30f3\u9178\u5316\u3057\u3001\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb- 3,4,5-\u4e09\u30ea\u30f3\u9178\uff08PtdIns[3,4,5]P3\u3001\u307e\u305f\u306fPIP3\uff09\u3092\u7523\u751f\u3055\u305b\u307e\u3059\u3002\u7d9a\u3044\u3066\u3001PIP3\u306e\u84c4\u7a4d\u3001\u306a\u3089\u3073\u306bAkt\u304a\u3088\u30733-\u30db\u30b9\u30db\u30a4\u30ce\u30b7\u30c1\u30c9\u4f9d\u5b58\u6027\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc1\uff08PDK1\uff09\u306a\u3069\u306e\u30d7\u30ec\u30af\u30b9\u30c8\u30ea\u30f3\u76f8\u540c\uff08PH\uff09\u30c9\u30e1\u30a4\u30f3\u3092\u6301\u3064\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u52d5\u54e1\u304c\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30ab\u30b9\u30b1\u30fc\u30c9\u3092\u8a98\u767a\u3057\u3001\u7d30\u80de\u6210\u9577\u304a\u3088\u3073\u751f\u5b58\u306a\u3089\u3073\u306b\u30ab\u30eb\u30b7\u30a6\u30e0\u52d5\u54e1\u3001\u7d30\u80de\u904b\u52d5\u6027\u3001\u5c0f\u80de\u8f38\u9001\u304a\u3088\u3073\u7d30\u80de\u5897\u6b96\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3001\u304a\u3088\u3073\u4ed6\u306e\u6a5f\u80fd\u3092\u542b\u3080\u591a\u304f\u306e\u305d\u306e\u4ed6\u306e\u91cd\u8981\u306a\u7d30\u80de\u6a5f\u80fd\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3057\u307e\u3059\uff08\u56f33.3\uff09<sup>*9<\/sup>\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"405\" height=\"258\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/8cc3e05d92150e42f33f9ee65f4f7f8a.jpg\" alt=\"\u30af\u30e9\u30b9I \u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb 3-\u30ad\u30ca\u30fc\u30bc (PI3K) \u306e\u7d30\u80de\u5185\u76f8\u4e92\u4f5c\u7528\" class=\"wp-image-21016\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/8cc3e05d92150e42f33f9ee65f4f7f8a.jpg 405w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/8cc3e05d92150e42f33f9ee65f4f7f8a-300x191.jpg 300w\" sizes=\"auto, (max-width: 405px) 100vw, 405px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.3. \u30af\u30e9\u30b9I \u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb 3-\u30ad\u30ca\u30fc\u30bc (PI3K) \u306e\u7d30\u80de\u5185\u76f8\u4e92\u4f5c\u7528<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">\u30af\u30e9\u30b9IA PI3K\u306f\u3001\u53d7\u5bb9\u4f53\u30c1\u30ed\u30b7\u30f3\u30ad\u30ca\u30fc\u30bc\u3068\u76f8\u4e92\u4f5c\u7528\u3057\u307e\u3059\u3002PI3K\u3092\u6d3b\u6027\u5316\u3059\u308b\u3068\u3001PIP2\u304c\u30ea\u30f3\u9178\u5316\u3055\u308c\u3001PIP3\u304c\u5408\u6210\u3055\u308c\u307e\u3059\u3002\u30af\u30e9\u30b9IA PI3K\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306b\u306f\u3001\u89e6\u5a92\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8:p110\u03b1\u3001p110\u03b2\u304a\u3088\u3073p110\u03b4\u304c\u542b\u307e\u308c\u3001\u305d\u308c\u305e\u308c\u304cPI3K\u306e\u8abf\u7bc0\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8p85\u3092\u542b\u6709\u3057\u307e\u3059\u3002\u30af\u30e9\u30b9IB PI3K\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0PI3K\u03b3\u306fG\u30bf\u30f3\u30d1\u30af\u8cea\u5171\u5f79\u53d7\u5bb9\u4f53\u3068\u76f8\u4e92\u4f5c\u7528\u3057\u3001\u89e6\u5a92\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8p110\u03b3\u304a\u3088\u3073\u8abf\u7bc0\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8p101\/p84\u3092\u542b\u6709\u3057\u307e\u3059\u3002\u3053\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30ab\u30b9\u30b1\u30fc\u30c9\u306e\u4f1d\u64ad\u306b\u3088\u3063\u3066\u3001\u91cd\u8981\u306a\u751f\u7269\u5b66\u7684\u6a5f\u80fd\u3092\u5236\u5fa1\u3059\u308b\u3055\u307e\u3056\u307e\u306a\u4e0b\u6d41\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u8abf\u7bc0\u3055\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u8102\u8cea\u30ad\u30ca\u30fc\u30bc\u306ePI3K\u306b\u306f\u30013\u7a2e\u985e\u306e\u30af\u30e9\u30b9\u304c\u7279\u5b9a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u56f33.3\u306b\u793a\u3057\u305f\u30af\u30e9\u30b9I PI3K\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u304c\u30d2\u30c8\u304c\u3093\u306b\u679c\u305f\u3059\u5f79\u5272\u306f\u3088\u304f\u8a8d\u8b58\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30af\u30e9\u30b9II PI3K\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\uff08\u6570\u7a2e\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u304c\u5b58\u5728\uff09\u306b\u3064\u3044\u3066\u306f\u3042\u307e\u308a\u89e3\u660e\u3055\u308c\u3066\u304a\u3089\u305a\u3001\u30d2\u30c8\u816b\u760d\u9032\u884c\u3068\u306e\u95a2\u9023\u6027\u306f\u5168\u304f\u77e5\u3089\u308c\u3066\u3044\u307e\u305b\u3093\u3002\u30af\u30e9\u30b9III PI3K\u3067\u552f\u4e00\u77e5\u3089\u308c\u3066\u3044\u308b\u306e\u306f\u3001\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u2015\u30ea\u30bd\u30bd\u30fc\u30e0\u5185\u3067\u7d30\u80de\u8cea\u6210\u5206\u3092\u5206\u89e3\u3059\u308b\u30b7\u30b9\u30c6\u30e0\u2015\u306e\u5236\u5fa1\u3067\u3001\u816b\u760d\u5f62\u6210\u306b\u304a\u3051\u308b\u4e2d\u5fc3\u7684\u5f79\u5272\u3092\u62c5\u3063\u3066\u3044\u307e\u3059\u3002PI3K\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u304c\u3093\u306e\u30c9\u30e9\u30a4\u30d0\u30fc\u5909\u7570\u306b\u306f\u3001\u89e6\u5a92\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u6d3b\u6027\u5316\u5909\u7570\u3084PI3K\u30d8\u30c6\u30ed\u4e8c\u91cf\u4f53\u306e\u8abf\u7bc0\u30c9\u30e1\u30a4\u30f3\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u6a5f\u80fd\u55aa\u5931\u578b\u5909\u7570\u304c\u542b\u307e\u308c\u307e\u3059<sup>*3&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<p>\u816b\u760d\u7d30\u80de\u9032\u884c\u306b\u95a2\u4e0e\u3059\u308b\u4e00\u822c\u7684\u306a\u53d7\u5bb9\u4f53\u30c1\u30ed\u30b7\u30f3\u30ad\u30ca\u30fc\u30bc\u306b\u3064\u3044\u3066\u306f\u7b2c2\u7bc0\u3067\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u591a\u304f\u306eG\u30bf\u30f3\u30d1\u30af\u8cea\u5171\u5f79\u53d7\u5bb9\u4f53\u306f\u3001\u5897\u6b96\u3084\u816b\u760d\u5897\u6b96\u306b\u5bc4\u4e0e\u3059\u308b\u4ed6\u306e\u30d7\u30ed\u30bb\u30b9\u306e\u8abf\u7bc0\u4e0d\u5168\u3068\u95a2\u4fc2\u3057\u3066\u3044\u308b\u3053\u3068\u306f\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\uff08\u88683.1\uff09<sup>*10&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<figure>\n<figcaption style=\"margin-bottom: 5px\"><b>\u88683.1.\u816b\u760d\u306b\u3088\u3063\u3066\u767a\u73fe\u3059\u308b\u4ee3\u8868\u7684\u306aGPCR<\/b><\/figcaption>\n<table style=\"border-collapse: collapse;border: 1px solid black;width: 440px;height: 286px\" border=\"1\" cellspacing=\"0\">\n<tbody>\n<tr style=\"background-color: #ff6600;color: white\">\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 150px\"><b>\u304c\u3093\u7a2e<\/b><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 256px\"><b>GPCR\/\u30ea\u30ac\u30f3\u30c9<\/b><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 150px\">\u4e73\u623f<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 256px\">GPER\/\u30a8\u30b9\u30c8\u30ed\u30b2\u30f3<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 150px\">\u7d50\u8178<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 256px\">PAR1\/\u30c8\u30ed\u30f3\u30d3\u30f3<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 150px\">\u975e\u5c0f\u7d30\u80de\u80ba<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 256px\">EP \u53d7\u5bb9\u4f53 \/PGE2<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 150px\">\u5375\u5de3<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 256px\">LPA1-LPA3\/LPA<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 150px\">\u524d\u7acb\u817a<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 256px\">AT1\/\u30a2\u30f3\u30b8\u30aa\u30c6\u30f3\u30b7\u30f3 II<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<figcaption style=\"font-size: small;margin-top: 5px\">AT, \u30a2\u30f3\u30b8\u30aa\u30c6\u30f3\u30b7\u30f3\uff1bEP, \u30d7\u30ed\u30b9\u30bf\u30b0\u30e9\u30f3\u30b8\u30f3 E\uff1bGPER, G\u30bf\u30f3\u30d1\u30af\u8cea\u5171\u5f79\u30a8\u30b9\u30c8\u30ed\u30b2\u30f3\u53d7\u5bb9\u4f53\uff1bLPA, \u30ea\u30be\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30f3\u9178\uff1bPAR, \u30d7\u30e9\u30c0\u30fc\u30fb\u30a6\u30a3\u30ea\u30fc\/\u30a2\u30f3\u30b8\u30a7\u30eb\u30de\u30f3\u9818\u57df\uff1bPGE2, \u30d7\u30ed\u30b9\u30bf\u30b0\u30e9\u30f3\u30b8\u30f3 E2.<\/figcaption>\n<\/figure>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td>\n<figure><img decoding=\"async\" class=\"size-full wp-image-21029\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_GPR30-Rabbit-Polyclonal-Antibofy.jpg\" alt=\"GPR30 Rabbit Polyclonal Antibody\" width=\"300\"><\/figure><div style=\"padding: 20px\"><\/div>\n<\/td><td><b>GPR30 Rabbit Polyclonal Antibody<\/b><br><small>\u6297GPR30\u6297\u4f53 (\u88fd\u54c1\u756a\u53f7 PA5-28647) \u3092\u4f7f\u7528\u3057\u305f\u3001\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3057\u305f\u30d2\u30c8\u5b50\u5bae\u5185\u819c\u7d44\u7e54\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3002<\/small><\/td><\/tr><tr><td>\n<figure><img decoding=\"async\" class=\"size-full wp-image-21029\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_LPA-Mouse-Monoclonal-Antibody-4H1.jpg\" alt=\"LPA Mouse Monoclonal Antibody (4H1)\" width=\"300\"><\/figure><div style=\"padding: 20px\"><\/div>\n<\/td><td><b>LPA Mouse Monoclonal Antibody (4H1)<\/b><br><small>\u6297LPA\u6297\u4f53 (\u7dd1\u8272) (\u88fd\u54c1\u756a\u53f7 MA5-17111) \u3067\u67d3\u8272\u3057\u305f\u3001HepG2 (\u4e0d\u6b7b\u5316\u30d2\u30c8\u809d\u7d30\u80de\u304c\u3093) \u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002\u6838\u306fDRAQ5\u2122 Dye (\u9752\u8272)\u3001\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f\u86cd\u5149\u30d5\u30a1\u30ed\u30a4\u30b8\u30f3\u30b3\u30f3\u30b8\u30e5\u30b2\u30fc\u30c8 (\u8d64\u8272) \u3067\u67d3\u8272\u3002<\/small><\/td><\/tr><tr><td>\n<figure><img decoding=\"async\" class=\"size-full wp-image-21029\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_Thrombin-Recepter-PAR1-Rabbit-Polyclonal-Antibody.jpg\" alt=\"Thrombin Receptor\/PAR1 Rabbit Polyclonal Antibody\" width=\"300\"><\/figure><div style=\"padding: 20px\"><\/div>\n<\/td><td><b>Thrombin Receptor\/PAR1 Rabbit Polyclonal Antibody<\/b><br><small>\u6297PAR1\u6297\u4f53 (\u88fd\u54c1\u756a\u53f7 PA5-34263) \u3092\u4f7f\u7528\u3057\u305f\u3001\u30db\u30eb\u30de\u30ea\u30f3\u56fa\u5b9a\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u30d2\u30c8\u9aa8\u9ac4\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3002<\/small><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>\u5f53\u8a18\u4e8b\u306f\u3001\u304c\u3093\u5897\u6b96\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306e\u63a2\u7d22\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002<br>\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\u306f\u4e0b\u8a18\u304b\u3089\u7121\u6599\u3067\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u9802\u3051\u307e\u3059\u3002<\/p>\n\n\n\n<p><strong><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/antibody-cancer-guidebook-form.html?CID=bid_pca_aup_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7121\u6599\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u304c\u3093\u306b\u304a\u3051\u308bpi3k\u306e\u5f79\u5272\">\u304c\u3093\u306b\u304a\u3051\u308bPI3K\u306e\u5f79\u5272<\/h2>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u30d2\u30c8\u816b\u760d\u306b\u95a2\u9023\u3059\u308bpi3k\u5909\u7570\">\u30d2\u30c8\u816b\u760d\u306b\u95a2\u9023\u3059\u308bPI3K\u5909\u7570<\/h4>\n\n\n\n<p>\u30af\u30e9\u30b9I PI3K\u3068\u304c\u3093\u3068\u306e\u95a2\u9023\u6027\u306b\u3064\u3044\u3066\u306f\u5e83\u304f\u5831\u544a\u3055\u308c\u3066\u304a\u308a\u3001\u3044\u304f\u3064\u304b\u306e\u3055\u307e\u3056\u307e\u306a\u30bf\u30a4\u30d7\u306e\u30d2\u30c8\u56fa\u5f62\u304c\u3093\u306b\u304a\u3051\u308bPI3K\u5909\u7570\u306e\u5f79\u5272\u304c\u5b9f\u8a3c\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3055\u307e\u3056\u307e\u306aPIK3CA\u5909\u7570\u306f\u975e\u30db\u30b8\u30ad\u30f3\u30ea\u30f3\u30d1\u816b\u3068\u3082\u95a2\u9023\u3057\u3066\u3044\u307e\u3059<sup>*11&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-pik3ca-\u89e6\u5a92\u30c9\u30e1\u30a4\u30f3-p110\u03b1-\u5909\u7570\u3092\u6301\u3064\u30d2\u30c8\u816b\u760d\u30bf\u30a4\u30d7\">PIK3CA\uff08\u89e6\u5a92\u30c9\u30e1\u30a4\u30f3 p110\u03b1\uff09\u5909\u7570\u3092\u6301\u3064\u30d2\u30c8\u816b\u760d\u30bf\u30a4\u30d7<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u4e73\u623f<\/li>\n\n\n\n<li>\u5b50\u5bae\u5185\u819c \u5c3f\u8def<\/li>\n\n\n\n<li>\u5b50\u5bae\u9838\u90e8 \u76ae\u819a<\/li>\n\n\n\n<li>\u5375\u5de3<\/li>\n\n\n\n<li>\u80c3<\/li>\n\n\n\n<li>\u80c6\u9053<\/li>\n\n\n\n<li>\u4e0a\u6c17\u9053<\/li>\n\n\n\n<li>\u5c0f\u8178<\/li>\n\n\n\n<li>\u98df\u9053<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-pik3r1-\u8abf\u7bc0\u30c9\u30e1\u30a4\u30f3-\u5909\u7570\u3092\u6301\u3064\u30d2\u30c8\u816b\u760d\u30bf\u30a4\u30d7\">PIK3R1\uff08\u8abf\u7bc0\u30c9\u30e1\u30a4\u30f3\uff09\u5909\u7570\u3092\u6301\u3064\u30d2\u30c8\u816b\u760d\u30bf\u30a4\u30d7<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5b50\u5bae\u5185\u819c \u7d50\u8178\u76f4\u8178 \u5b50\u5bae\u9838\u90e8<\/li>\n\n\n\n<li>\u4e0a\u6c17\u9053<\/li>\n\n\n\n<li>\u4e2d\u67a2\u795e\u7d4c\u7cfb<\/li>\n\n\n\n<li>\u9ac4\u819c<\/li>\n\n\n\n<li>\u4e73\u623f<\/li>\n\n\n\n<li>\u98df\u9053<\/li>\n\n\n\n<li>\u5c3f\u8def<\/li>\n\n\n\n<li>\u80ba<\/li>\n\n\n\n<li>\u5375\u5de3<\/li>\n\n\n\n<li>\u809d\u81d3<\/li>\n<\/ul>\n\n\n\n<p>\u30ea\u30b9\u30c8\u306e\u4e26\u3073\u9806\u306f\u3001PI3K\u5909\u7570\u306e\u691c\u51fa\u983b\u5ea6\u304c\u9ad8\u3044\u304c\u3093\u7a2e\u304b\u3089\u9806\u756a\u306b\u4e0a\u304b\u3089\u4e0b\u3078\u5411\u304b\u3063\u3066\u8868\u793a\u3057\u3066\u3044\u307e\u3059<sup>*3&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td>\n<figure><img decoding=\"async\" class=\"size-full wp-image-21066\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PIK3R1-Mouse-Monoclonal-Antibody-6G10.jpg\" alt=\"PIK3R1 Mouse Monoclonal Antibody (6G10)\" width=\"300\"><\/figure><div style=\"padding: 20px\"><\/div>\n<\/td><td><b>PIK3R1 Mouse Monoclonal Antibody (6G10)<\/b><br><small>\u6297PIK3R1\u6297\u4f53 (\u7dd1\u8272) (\u88fd\u54c1\u756a\u53f7 MA1-74183) \u3067\u67d3\u8272\u3057\u305f\u3001HeLa (\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093) \u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002\u6838\u306fDRAQ5\u2122 Dye (\u9752\u8272)\u3001\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f\u86cd\u5149\u30d5\u30a1\u30ed\u30a4\u30b8\u30f3\u30b3\u30f3\u30b8\u30e5\u30b2\u30fc\u30c8 (\u8d64\u8272) \u3067\u67d3\u8272\u3002<\/small><\/td><\/tr><tr><td>\n<figure><img decoding=\"async\" class=\"size-full wp-image-21067\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PI3-Kinase-p110-alpha-Rabbit-Monoclonal-Antibody-H.843.0.jpg\" alt=\"PI3 Kinase p110-alpha Rabbit Monoclonal Antibody (H.843.0)\" width=\"300\"><\/figure><div style=\"padding: 20px\"><\/div>\n<\/td><td><b>PI3 Kinase p110-alpha Rabbit Monoclonal Antibody (H.843.0)<\/b><br><small>\u6297PI3\u30ad\u30ca\u30fc\u30bcp110\u03b1\u6297\u4f53 (\u88fd\u54c1\u756a\u53f7 MA5-15255) \u3092\u4f7f\u7528\u3057\u305f\u3001\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3057\u305f\u30d2\u30c8\u4e73\u304c\u3093\u7d44\u7e54\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3002<\/small><\/td><\/tr><tr><td>\n<figure><img decoding=\"async\" class=\"size-full wp-image-21068\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PI3-Kinase-p85-Alpha-Rabbit-Polyclonal-Antibody.jpg\" alt=\"PI3 Kinase p85 Alpha Rabbit Polyclonal Antibody\" width=\"300\"><\/figure><div style=\"padding: 20px\"><\/div>\n<\/td><td><b>PI3 Kinase p85 Alpha Rabbit Polyclonal Antibody<\/b><br><small>\u6297PI3\u30ad\u30ca\u30fc\u30bcp85\u03b1\u6297\u4f53 (\u88fd\u54c1\u756a\u53f7 PA1-14343) \u3092\u4f7f\u7528\u3057\u305f\u3001\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3057\u305f\u30d2\u30c8\u4e73\u304c\u3093\u7d44\u7e54\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3002<\/small><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>\u5f53\u793e\u306f\u3001\u3053\u306e\u4e3b\u8981\u306a\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306ePI3K\u304a\u3088\u3073\u30e1\u30c7\u30a3\u30a8\u30fc\u30bf\u30fc\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u5e45\u5e83\u3044\u6297\u4f53\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u8a73\u3057\u3044\u60c5\u5831\u306f<a href=\"http:\/\/www.thermofisher.com\/order\/genome-database\/searchResults?searchMode=keyword&amp;CID=&amp;productTypeSelect=antibody&amp;alternateProductTypeSelect=&amp;originalCount=&amp;alternateTargetTypeSelect=&amp;targetTypeSelect=antibody_primary&amp;species=ltechall&amp;otherSpecies=&amp;selectedInputType=&amp;keyword=PI3K&amp;sequenceInput=&amp;chromStart=&amp;chromStop=&amp;batchText=&amp;CID=fl-PI3K&amp;CID=bid_pca_aup_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3053\u3061\u3089<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><strong>PIK3CA Mouse Monoclonal Antibody (4F3)<\/strong><br>\u88fd\u54c1\u756a\u53f7 MA5-17149<br>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\uff1aFC\u3001IF\u3001WB<br>PI3K\u306f\u3001\u304c\u3093\u907a\u4f1d\u5b50\u3068\u3057\u3066\u6a5f\u80fd\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u7d50\u8178\u3001\u8133\u3001\u4e73\u623f\u3001\u80c3\u3001\u80ba\u3001\u304a\u3088\u3073\u4ed6\u306e\u5668\u5b98\u306e\u304c\u3093\u306b\u304a\u3044\u3066\u3001\u4f53\u7d30\u80de\u5909\u7570\uff08PI3K\u907a\u4f1d\u5b50\u5ea7\u306b\u304a\u3051\u308b\u7279\u7570\u7684\u5897\u5e45\u304a\u3088\u3073\u907a\u4f1d\u5b50\u5185\u306e\u6b20\u5931\uff09\u304c\u3001PI3K\uff08PIK3CA\uff09\u306e\u89e6\u5a92\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8110\u03b1\u306e\u6d3b\u6027\u306e\u4e0a\u6607\u3092\u8a98\u5c0e\u3059\u308b\u3053\u3068\u306f\u591a\u304f\u306e\u7814\u7a76\u3067\u78ba\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059&nbsp;<sup>*12&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<div style=\"text-align: center;margin: 0 auto\">\n\n<img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-21016\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-72-e1748266850407.jpg\" alt=\"PIK3CA\u306b\u5bfe\u3059\u308b\u6297\u4f53\u3092\u4f7f\u7528\u3057\u305f\u3001\uff08A\uff09Jurkat\uff08\u4e0d\u6b7b\u5316\u6025\u6027T\u7d30\u80de\u767d\u8840\u75c5\uff09\u7d30\u80de\uff08\u7dd1\u8272\uff09\u304a\u3088\u3073\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff08\u8d64\u8272\uff09\u306e\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u89e3\u6790\u3001\uff08B\uff09HeLa\uff08\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093\uff09\u7d30\u80de\uff08\u7dd1\u8272\uff09\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3001\uff08C\uff09\u30d2\u30c8PIK3CA\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u691c\u51fa\u3001\u304a\u3088\u3073\uff08D\uff091\uff09HEK293\uff08\u4e0d\u6b7b\u5316\u30d2\u30c8\u814e\u81d3\uff09\u306a\u3089\u3073\u306b 2\uff09PIK3CA\u3092\u5c0e\u5165\u3057\u305fHEK293\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u306b\u304a\u3051\u308bPIK3CA\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\u3002\" width=\"410\" height=\"520\">\n\n<p><small><b>PIK3CA\u306b\u5bfe\u3059\u308b\u6297\u4f53<\/b>\u3092\u4f7f\u7528\u3057\u305f\u3001\uff08A\uff09Jurkat\uff08\u4e0d\u6b7b\u5316\u6025\u6027T\u7d30\u80de\u767d\u8840\u75c5\uff09\u7d30\u80de\uff08\u7dd1\u8272\uff09\u304a\u3088\u3073\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff08\u8d64\u8272\uff09\u306e\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u89e3\u6790\u3001\uff08B\uff09HeLa\uff08\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093\uff09\u7d30\u80de\uff08\u7dd1\u8272\uff09\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3001\uff08C\uff09\u30d2\u30c8PIK3CA\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u691c\u51fa\u3001\u304a\u3088\u3073\uff08D\uff091\uff09HEK293\uff08\u4e0d\u6b7b\u5316\u30d2\u30c8\u814e\u81d3\uff09\u306a\u3089\u3073\u306b 2\uff09PIK3CA\u3092\u5c0e\u5165\u3057\u305fHEK293\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u306b\u304a\u3051\u308bPIK3CA\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\u3002<\/small><\/p>\n<p>&nbsp;<\/p>\n\n\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u30c6\u30f3\u30b7\u30f3\u30db\u30e2\u30ed\u30b0-pten\">\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u30c6\u30f3\u30b7\u30f3\u30db\u30e2\u30ed\u30b0(PTEN)<\/h2>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-pten\u306e\u69cb\u9020-\u6a5f\u80fd\u304a\u3088\u3073\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\">PTEN\u306e\u69cb\u9020\u3001\u6a5f\u80fd\u304a\u3088\u3073\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054<\/h4>\n\n\n\n<p>\u816b\u760d\u6291\u5236\u56e0\u5b50PTEN\u306f\u3001PI3K\u3092\u4ecb\u3059\u308b\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306e\u6d3b\u6027\u3092\u6291\u5236\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001Akt\u3092\u963b\u5bb3\u3057\u307e\u3059\u3002PTEN\u306f\u3001\u8102\u8cea\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u3068\u3057\u3066\u6a5f\u80fd\u3057\u3001PIP3\u306e\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb\u74b0\u306e3\u4f4d\u306e\u6c34\u9178\u57fa\u3092\u8131\u30ea\u30f3\u9178\u5316\u3057\u3001PIP2\u3092\u751f\u6210\u3057\u307e\u3059\u3002PTEN\u306f\u3001PI3K\/Akt\/mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30ab\u30b9\u30b1\u30fc\u30c9\u306e\u8ca0\u306e\u5236\u5fa1\u56e0\u5b50\u3068\u3057\u3066\u3001\u5897\u6b96\u3001\u751f\u5b58\u3001\u304a\u3088\u3073\u30a8\u30cd\u30eb\u30ae\u30fc\u4ee3\u8b1d\u306a\u3069\u306e\u57fa\u672c\u7684\u306a\u7d30\u80de\u6a5f\u80fd\u306e\u5236\u5fa1\u306b\u304a\u3044\u3066\u6975\u3081\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u307e\u3059\uff08\u56f33.4\u304a\u3088\u30733.5\uff09<sup>*11,13<\/sup> \u3002PTEN\u306f\u3001PIP3\u3068\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u4ed6\u306b\u3001PI3K\u304a\u3088\u3073\u63a5\u7740\u6591\u30ad\u30ca\u30fc\u30bc\uff08FAK\uff09\u306a\u3069\u306e\u30c1\u30ed\u30b7\u30f3\u30ea\u30f3\u9178\u57fa\u3092\u6301\u3064\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3082\u8a8d\u8b58\u3057\u3001\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30bf\u30f3\u30d1\u30af\u8ceaShc\u306a\u3069\u306e\u30bb\u30ea\u30f3\u6b8b\u57fa\u304a\u3088\u3073\u30b9\u30ec\u30aa\u30cb\u30f3\u6b8b\u57fa\u3092\u6301\u3064\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5bfe\u3059\u308b\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u3068\u3057\u3066\u3082\u4f5c\u7528\u3057\u307e\u3059<sup>*14&nbsp;&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"407\" height=\"408\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/703bf0d43a8164d25f2f5e482db89596.jpg\" alt=\"\u56f33.4. \u8abf\u7bc0\u7d4c\u8def\u306b\u304a\u3051\u308bPTEN\u306e\u5f79\u5272\uff1a\u816b\u760d\u7d30\u80de\u306b\u3088\u308bPTEN\u8abf\u7bc0\u306e\u55aa\u5931\u306f\u3001\u591a\u304f\u306e\u30bf\u30a4\u30d7\u306e\u56fa\u5f62\u816b\u760d\u304a\u3088\u3073\u8840\u6db2\u7cfb\u816b\u760d\u306b\u304a\u3051\u308bAkt\u6d3b\u6027\u5316\u306e\u5897\u52a0\u306b\u5bc4\u4e0e\u3057\u307e\u3059\u3002\" class=\"wp-image-21166\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/703bf0d43a8164d25f2f5e482db89596.jpg 407w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/703bf0d43a8164d25f2f5e482db89596-300x300.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/703bf0d43a8164d25f2f5e482db89596-150x150.jpg 150w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/703bf0d43a8164d25f2f5e482db89596-80x80.jpg 80w\" sizes=\"auto, (max-width: 407px) 100vw, 407px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.4. \u8abf\u7bc0\u7d4c\u8def\u306b\u304a\u3051\u308bPTEN\u306e\u5f79\u5272<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">\u816b\u760d\u7d30\u80de\u306b\u3088\u308bPTEN\u8abf\u7bc0\u306e\u55aa\u5931\u306f\u3001\u591a\u304f\u306e\u30bf\u30a4\u30d7\u306e\u56fa\u5f62\u816b\u760d\u304a\u3088\u3073\u8840\u6db2\u7cfb\u816b\u760d\u306b\u304a\u3051\u308bAkt\u6d3b\u6027\u5316\u306e\u5897\u52a0\u306b\u5bc4\u4e0e\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"426\" height=\"343\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/9adffdf6c1d26a4bf52afaf9f19bcfc0.jpg\" alt=\"\u56f33.5. PTEN\u306e\u69cb\u9020\uff1a\u3053\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306f403\u30a2\u30df\u30ce\u9178\u304b\u3089\u69cb\u6210\u3055\u308c\u307e\u3059\u3002\u4e3b\u8981\u306a\u6a5f\u80fd\u30c9\u30e1\u30a4\u30f3\u306b\u306f\u3001N\u672b\u7aefPIP2\u7d50\u5408\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u30c9\u30e1\u30a4\u30f3\uff08\u8d64\uff09\u3001C2\u30c9\u30e1\u30a4\u30f3\u3001\u304a\u3088\u3073PTEN\u5236\u5fa1\u306b\u95a2\u4e0e\u3059\u308bC\u672b\u7aef\u30c6\u30fc\u30eb\uff08\u9752\uff09\u304c\u542b\u307e\u308c\u307e\u3059\u3002\u816b\u760d\u691c\u4f53\u306b\u304a\u3044\u3066\u691c\u51fa\u3055\u308c\u308b\u4e3b\u8981\u306aPTEN\u30df\u30b9\u30bb\u30f3\u30b9\u5909\u7570\u306f\u3001\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u30c9\u30e1\u30a4\u30f3\u3092\u6a19\u7684\u3068\u3057\u3001PTEN\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u6d3b\u6027\u306e\u55aa\u5931\u3092\u3082\u305f\u3089\u3057\u307e\u3059\u3002\" class=\"wp-image-21167\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/9adffdf6c1d26a4bf52afaf9f19bcfc0.jpg 426w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/9adffdf6c1d26a4bf52afaf9f19bcfc0-300x242.jpg 300w\" sizes=\"auto, (max-width: 426px) 100vw, 426px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.5. PTEN\u306e\u69cb\u9020<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">\u3053\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306f403\u30a2\u30df\u30ce\u9178\u304b\u3089\u69cb\u6210\u3055\u308c\u307e\u3059\u3002\u4e3b\u8981\u306a\u6a5f\u80fd\u30c9\u30e1\u30a4\u30f3\u306b\u306f\u3001N\u672b\u7aefPIP2\u7d50\u5408\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u30c9\u30e1\u30a4\u30f3\uff08\u8d64\uff09\u3001C2\u30c9\u30e1\u30a4\u30f3\u3001\u304a\u3088\u3073PTEN\u5236\u5fa1\u306b\u95a2\u4e0e\u3059\u308bC\u672b\u7aef\u30c6\u30fc\u30eb\uff08\u9752\uff09\u304c\u542b\u307e\u308c\u307e\u3059\u3002\u816b\u760d\u691c\u4f53\u306b\u304a\u3044\u3066\u691c\u51fa\u3055\u308c\u308b\u4e3b\u8981\u306aPTEN\u30df\u30b9\u30bb\u30f3\u30b9\u5909\u7570\u306f\u3001\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u30c9\u30e1\u30a4\u30f3\u3092\u6a19\u7684\u3068\u3057\u3001PTEN\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u6d3b\u6027\u306e\u55aa\u5931\u3092\u3082\u305f\u3089\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"321\" height=\"166\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PTEN-Mouse-Monoclonal-Antibody-17.A.jpg\" alt=\"PTEN Mouse Monoclonal Antibody (17.A)\uff1a\u6297PTEN\u6297\u4f53\uff08\u88fd\u54c1\u756a\u53f7 MA5-12278\uff09\u3092\u4f7f\u7528\u3057\u305f\u3001MCF-7\uff08\u4e0d\u6b7b\u5316\u30d2\u30c8\u4e73\u817a\u304c\u3093\uff09\u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002(\u53f3) \u7d30\u80de\u8cea\u5185\u306ePTEN\uff08\u7dd1\u8272\uff09\u3068\uff08\u5de6\uff09\u4e00\u6b21\u6297\u4f53\u4e0d\u4f7f\u7528\u306e\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u306e\u6bd4\u8f03\u3002\" class=\"wp-image-21169\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PTEN-Mouse-Monoclonal-Antibody-17.A.jpg 321w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PTEN-Mouse-Monoclonal-Antibody-17.A-300x155.jpg 300w\" sizes=\"auto, (max-width: 321px) 100vw, 321px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>PTEN Mouse Monoclonal Antibody (17.A)<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">\u6297PTEN\u6297\u4f53\uff08\u88fd\u54c1\u756a\u53f7 MA5-12278\uff09\u3092\u4f7f\u7528\u3057\u305f\u3001MCF-7\uff08\u4e0d\u6b7b\u5316\u30d2\u30c8\u4e73\u817a\u304c\u3093\uff09\u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002(\u53f3) \u7d30\u80de\u8cea\u5185\u306ePTEN\uff08\u7dd1\u8272\uff09\u3068\uff08\u5de6\uff09\u4e00\u6b21\u6297\u4f53\u4e0d\u4f7f\u7528\u306e\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u306e\u6bd4\u8f03\u3002<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u304c\u3093\u306b\u304a\u3051\u308bpten\u306e\u5f79\u5272\">\u304c\u3093\u306b\u304a\u3051\u308bPTEN\u306e\u5f79\u5272<\/h4>\n\n\n\n<p>PTEN\u5909\u7570\u3092\u6301\u3064\u30d2\u30c8\u304c\u3093<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5b50\u5bae\u5185\u819c<\/li>\n\n\n\n<li>\u4e2d\u67a2\u795e\u7d4c\u7cfb<\/li>\n\n\n\n<li>\u76ae\u819a<\/li>\n\n\n\n<li>\u7d50\u8178\u76f4\u8178<\/li>\n\n\n\n<li>\u524d\u7acb\u817a<\/li>\n\n\n\n<li>\u80c6\u9053<\/li>\n\n\n\n<li>\u553e\u6db2\u817a<\/li>\n\n\n\n<li>\u773c<\/li>\n\n\n\n<li>\u5b50\u5bae\u9838\u90e8<\/li>\n\n\n\n<li>\u4e73\u623f<\/li>\n\n\n\n<li>\u5375\u5de3<\/li>\n\n\n\n<li>\u80c3<\/li>\n<\/ul>\n\n\n\n<p>PTEN\u8abf\u7bc0\u306e\u55aa\u5931\u306b\u3064\u306a\u304c\u308b\u751f\u6b96\u7d30\u80de\u7cfb\u5217\u5909\u7570\u306f\u3001\u30ab\u30a6\u30c7\u30f3\u75c7\u5019\u7fa4\u3084\u30d0\u30ca\u30e4\u30f3\u30fb\u30e9\u30a4\u30ea\u30fc\u30fb\u30eb\u30d0\u30eb\u30ab\u30d0\u75c7\u5019\u7fa4\u306a\u3069\u306e\u907a\u4f1d\u6027\u75be\u60a3\u306b\u95a2\u4fc2\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u72b6\u614b\u306e\u5f71\u97ff\u3092\u53d7\u3051\u305f\u500b\u4f53\u306f\u3001\u76ae\u819a\u3084\u8eab\u4f53\u306e\u3055\u307e\u3056\u307e\u306a\u5668\u5b98\u306b\u975e\u304c\u3093\u6027\u75c5\u5909\u3092\u767a\u73fe\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u907a\u4f1d\u6027\u75be\u60a3\u306f\u3001\u7279\u5b9a\u306e\u30bf\u30a4\u30d7\u306e\u60aa\u6027\u816b\u760d\u306e\u767a\u73fe\u53ef\u80fd\u6027\u306e\u5897\u52a0\u306b\u3082\u95a2\u4fc2\u3057\u307e\u3059\u3002\u591a\u6570\u306e\u30d2\u30c8\u304c\u3093\u306b\u304a\u3044\u3066\u3001\u30db\u30e2\u6b20\u5931\u3001\u30c8\u30e9\u30f3\u30b1\u30fc\u30b7\u30e7\u30f3\u5909\u7570\u3001\u70b9\u5909\u7570\u3001\u304a\u3088\u3073\u4ed6\u306e\u7570\u5e38\u3092\u542b\u3080\u4f53\u7d30\u80de\u5909\u7570\u304c\u540c\u5b9a\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002PTEN\u306f\u3001\u816b\u760d\u6291\u5236\u56e0\u5b50p53\u3068\u540c\u69d8\u306b\u3001\u6700\u3082\u4e00\u822c\u7684\u306a\u304c\u3093\u95a2\u9023\u907a\u4f1d\u5b50\u306e\u4e00\u3064\u3067\u3059\uff08\u56f33.5\uff09<sup>*3,15&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<p><strong>\u304c\u3093\u306b\u304a\u3051\u308bPTEN\u306e\u5f79\u5272<\/strong><br>\u5f53\u793e\u306f\u3001PTEN\u304a\u3088\u3073\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u306e\u76f4\u63a5\u6a19\u7684\u3001\u306a\u3089\u3073\u306bPI3K\/Akt\/mTOR\u7d4c\u8def\u304c\u95a2\u4fc2\u3059\u308b\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u5e45\u5e83\u3044\u6297\u4f53\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u8a73\u3057\u3044\u60c5\u5831\u306f<strong><a href=\"http:\/\/www.thermofisher.com\/PTEN?CID=bid_pca_aup_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab13_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">\u3053\u3061\u3089<\/a><\/strong>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><strong>SHC1 Rabbit Polyclonal Antibody<\/strong><br>\u88fd\u54c1\u756a\u53f7 PA5-27310<br>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\uff1aWB\u3001IHC (P)\u3001 IF<br>PTEN\u3068PI3K\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u306b\u3064\u3044\u3066\u306f\u5341\u5206\u306b\u89e3\u660e\u3055\u308c\u3066\u3044\u308b\u306e\u306b\u52a0\u3048\u3066\u3001\u30a2\u30c0\u30d7\u30bf\u30fc\u5206\u5b50SH2\u30c9\u30e1\u30a4\u30f3\u30bf\u30f3\u30d1\u30af\u8ceaC1\uff08Shc1\uff09\u306f\u3001PTEN\u306e\u76f4\u63a5\u306e\u57fa\u8cea\u3067\u3059\u30023\u7a2e\u985e\u306e\u4e3b\u8981\u306a\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u304cShc\u907a\u4f1d\u5b50\u306b\u3088\u3063\u3066\u30b3\u30fc\u30c9\u3055\u308c\u3066\u3044\u307e\u3059\u3002p52Shc \u304a\u3088\u3073p46Shc\u306f\u3001\u5897\u6b96\u56e0\u5b50\u53d7\u5bb9\u4f53\u306e\u4e0b\u6d41\u3067\u6a5f\u80fd\u3057\u3001Ras\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u3092\u4ecb\u3059\u308b\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3092\u4fc3\u9032\u3057\u307e\u3059<sup>*16<\/sup> \u3002p66Shc\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306f\u3001Shc\u306b\u3088\u308b\u6d3b\u6027\u9178\u7d20\u7a2e\u306e\u5236\u5fa1\u3092\u4ecb\u3059\u308b\u7d30\u80de\u5bff\u547d\u306e\u5ef6\u9577\u306b\u95a2\u4fc2\u3057\u307e\u3059\u3002\u3044\u304f\u3064\u304b\u306e\u30bf\u30a4\u30d7\u306e\u56fa\u5f62\u304c\u3093\u306b\u304a\u3044\u3066\u3001Shc1\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u304c\u304c\u3093\u306e\u9032\u884c\u306b\u95a2\u4e0e\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u8907\u6570\u306e\u7814\u7a76\u3067\u793a\u3055\u308c\u3066\u3044\u307e\u3059&nbsp;<sup>*17&nbsp;<\/sup>\u3002\u8a73\u7d30\u306a\u6280\u8853\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u304a\u3088\u3073\u30ea\u30bd\u30fc\u30b9\u306f\u4ed8\u9332\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<div class=\"wp-block-media-text is-stacked-on-mobile is-vertically-aligned-center\" style=\"grid-template-columns:30% auto\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"184\" height=\"165\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_PTEN-Rabbit-Monoclonal-Antibody-PN37.jpg\" alt=\"PTEN Rabbit Monoclonal Antibody (PN37)\uff1a\u6297PTEN\u6297\u4f53\uff08\u88fd\u54c1\u756a\u53f7 18-0256\uff09\u3092\u4f7f\u7528\u3057\u305f\u3001\u30d2\u30c8\u524d\u7acb\u817a\u7d44\u7e54\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u67d3\u8272\u3002\" class=\"wp-image-21173 size-full\" \/><\/figure><div class=\"wp-block-media-text__content\">\n<p><strong>PTEN Rabbit Monoclonal Antibody (PN37)<\/strong><\/p>\n\n\n\n<p class=\"has-small-font-size\">\u6297PTEN\u6297\u4f53\uff08\u88fd\u54c1\u756a\u53f7 18-0256\uff09\u3092\u4f7f\u7528\u3057\u305f\u3001\u30d2\u30c8\u524d\u7acb\u817a\u7d44\u7e54\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u67d3\u8272\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"370\" height=\"385\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/3a613c78d880dfc72f3f1373bd7f0664.jpg\" alt=\"SHC1\u306b\u5bfe\u3059\u308b\u6297\u4f53\u3092\u4f7f\u7528\u3057\u305f\u3001\uff08A\uff09A431\uff08\u4e0d\u6b7b\u5316\u30d2\u30c8\u985e\u8868\u76ae\u304c\u3093\uff09\u7d30\u80de\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\u3001\uff08B\uff09\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3057\u305f\u7d50\u8178\u304c\u3093\u5207\u7247\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3001\uff08C\uff09A431\u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3001\u304a\u3088\u3073\uff08D\uff09NIH\/3T3\uff08\u4e0d\u6b7b\u5316\u30de\u30a6\u30b9\u80da\u7dda\u7dad\u82bd\u7d30\u80de\uff09\u7d30\u80de\u7531\u6765SHC1\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u691c\u51fa\" class=\"wp-image-21175\" style=\"width:462px;height:auto\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/3a613c78d880dfc72f3f1373bd7f0664.jpg 370w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/3a613c78d880dfc72f3f1373bd7f0664-288x300.jpg 288w\" sizes=\"auto, (max-width: 370px) 100vw, 370px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\"><strong>SHC1\u306b\u5bfe\u3059\u308b\u6297\u4f53<\/strong>\u3092\u4f7f\u7528\u3057\u305f\u3001\uff08A\uff09A431\uff08\u4e0d\u6b7b\u5316\u30d2\u30c8\u985e\u8868\u76ae\u304c\u3093\uff09\u7d30\u80de\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\u3001\uff08B\uff09\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3057\u305f\u7d50\u8178\u304c\u3093\u5207\u7247\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3001\uff08C\uff09A431\u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3001\u304a\u3088\u3073\uff08D\uff09NIH\/3T3\uff08\u4e0d\u6b7b\u5316\u30de\u30a6\u30b9\u80da\u7dda\u7dad\u82bd\u7d30\u80de\uff09\u7d30\u80de\u7531\u6765SHC1\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u691c\u51fa<\/p>\n<\/div>\n\n\n\n<p>\u5f53\u8a18\u4e8b\u306f\u3001\u304c\u3093\u5897\u6b96\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306e\u63a2\u7d22\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002<br>\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\u306f\u4e0b\u8a18\u304b\u3089\u7121\u6599\u3067\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u9802\u3051\u307e\u3059\u3002<\/p>\n\n\n\n<p><strong><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/antibody-cancer-guidebook-form.html?CID=bid_pca_aup_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7121\u6599\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-akt-\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bcb\">Akt\/\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bcB<\/h2>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-akt\u306e\u69cb\u9020-\u6a5f\u80fd\u304a\u3088\u3073\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\">Akt\u306e\u69cb\u9020\u3001\u6a5f\u80fd\u304a\u3088\u3073\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054<\/h4>\n\n\n\n<p>\u30bb\u30ea\u30f3\/\u30b9\u30ec\u30aa\u30cb\u30f3\u30ad\u30ca\u30fc\u30bcAkt\uff08\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bcB\u3068\u3082\u547c\u3070\u308c\u308b\uff09\u306f\u3001\u5e83\u7bc4\u56f2\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u306e\u4e2d\u5fc3\u7684\u5b58\u5728\uff08\u53ce\u675f\u30ce\u30fc\u30c9\uff09\u3067\u3059\u3002\u672c\u9175\u7d20\u306f\u3001\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u306eAGC\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u30e1\u30f3\u30d0\u30fc\u3067\u300160\u7a2e\u985e\u4ee5\u4e0a\u306e\u30d2\u30c8\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u304c\u542b\u307e\u308c\u307e\u3059\u3002Akt\u6d3b\u6027\u5316\u306f\u3053\u308c\u3089\u306e\u7d30\u80de\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306e\u30de\u30b9\u30bf\u30fc\u30b9\u30a4\u30c3\u30c1\u3068\u3057\u3066\u6a5f\u80fd\u3057\u3001\u3055\u307e\u3056\u307e\u306a\u4e0b\u6d41\u30bf\u30fc\u30b2\u30c3\u30c8\u3084\u76f8\u4e92\u4f5c\u7528\u30d1\u30fc\u30c8\u30ca\u30fc\u3092\u4ecb\u3057\u3066\u6570\u591a\u304f\u306e\u7d30\u80de\u5185\u53cd\u5fdc\u3092\u5f15\u304d\u8d77\u3053\u3057\u307e\u3059\u3002Akt\u306f\u7d30\u80de\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306b\u304a\u3051\u308b\u4e2d\u5fc3\u7684\u5f79\u5272\u3092\u679c\u305f\u3057\u3001\u3053\u308c\u3089\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306e\u7570\u5e38\u306f\u304c\u3093\u304a\u3088\u3073\u7cd6\u5c3f\u75c5\u304b\u3089\u795e\u7d4c\u5909\u6027\u306b\u81f3\u308b\u5e83\u7bc4\u56f2\u306e\u75be\u60a3\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3057\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001Akt\u306f\u3001\u57fa\u790e\u7814\u7a76\u304a\u3088\u3073\u85ac\u5264<br>\u958b\u767a\u306e\u4e21\u5206\u91ce\u306b\u304a\u3044\u3066\u6700\u3082\u7a4d\u6975\u7684\u306b\u7814\u7a76\u3055\u308c\u3066\u3044\u308b\u30ad\u30ca\u30fc\u30bc\u306e\u4e00\u3064\u3067\u3059\uff08\u56f33.6\uff09<sup>*19&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"366\" height=\"360\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-76-e1748269623858.jpg\" alt=\"\u56f33.6. Akt\u306f\u30de\u30b9\u30bf\u30fc\u5236\u5fa1\u56e0\u5b50\uff1aAkt\u7d4c\u8def\u306e\u6d3b\u6027\u5316\u306f\u5e45\u5e83\u3044\u751f\u547d\u7d30\u80de\u6a5f\u80fd\u306b\u5f71\u97ff\u3002\" class=\"wp-image-3980\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-76-e1748269623858.jpg 366w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-76-e1748269623858-300x295.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-76-e1748269623858-80x80.jpg 80w\" sizes=\"auto, (max-width: 366px) 100vw, 366px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.6. Akt\u306f\u30de\u30b9\u30bf\u30fc\u5236\u5fa1\u56e0\u5b50<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">Akt\u7d4c\u8def\u306e\u6d3b\u6027\u5316\u306f\u5e45\u5e83\u3044\u751f\u547d\u7d30\u80de\u6a5f\u80fd\u306b\u5f71\u97ff\u3002<\/p>\n\n\n\n<p>Akt\u306f\u3001Akt1\/PKB\u03b1\u3001Akt2\/PKB\u03b2\u3001\u304a\u3088\u3073Akt3\/PKB\u03b3\u306e3\u7a2e\u985e\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u3068\u3057\u3066\u767a\u73fe\u3057\u307e\u3059\u30023\u7a2e\u985e\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306b\u306f\u5168\u3066\u306b\u3001\u30a2\u30df\u30ce\u672b\u7aef\u30d7\u30ec\u30af\u30b9\u30c8\u30ea\u30f3\u76f8\u540c\uff08PH\uff09\u30c9\u30e1\u30a4\u30f3\u3001\u4e2d\u592e\u306e\u30bb\u30ea\u30f3\/\u30b9\u30ec\u30aa\u30cb\u30f3\u89e6\u5a92\u30c9\u30e1\u30a4\u30f3\u3001\u304a\u3088\u3073\u5c0f\u3055\u306a\u30ab\u30eb\u30dc\u30ad\u30b7\u30eb\u672b\u7aef\u8abf\u7bc0\u30c9\u30e1\u30a4\u30f3\u304c\u5b58\u5728\u3057\u307e\u3059\u3002PH\u30c9\u30e1\u30a4\u30f3\u306f\u3001PI3K\u7523\u7269\u3067\u3042\u308bPIP2\u304a\u3088\u3073PIP3\u306b\u7d50\u5408\u3057\u307e\u3059\uff08\u56f33.7\uff09<sup>*18,19<\/sup>\u3002\u3053\u308c\u3089\u306e\u76f8\u4e92\u4f5c\u7528\u306b\u3088\u308aAkt\u306e\u7d30\u80de\u819c\u3078\u306e\u79fb\u884c\u304c\u8a98\u5c0e\u3055\u308c\u3001\u30db\u30b9\u30db\u30a4\u30ce\u30b7\u30c1\u30c9\u4f9d\u5b58\u6027\u30ad\u30ca\u30fc\u30bc1\uff08PDK1\uff09\u306b\u3088\u3063\u3066\u89e6\u5a92\u30c9\u30e1\u30a4\u30f3\u306e\u6d3b\u6027\u5316\u30eb\u30fc\u30d7\u4e2d\u306eThr308\u304c\u30ea\u30f3\u9178\u5316\u3055\u308c\u307e\u3059&nbsp;<sup>*20&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<p>\u5b8c\u5168\u306a\u6d3b\u6027\u5316\u306b\u306f\u3001\u7b2c\u4e8c\u306e\u90e8\u4f4dSer473\u306e\u30ea\u30f3\u9178\u5316\u53cd\u5fdc\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002\u73fe\u5728\u306e\u7814\u7a76\u3067\u306f\u3001mTOR\u2013Rictor\u8907\u5408\u4f53\uff08mTORC2\uff09\u304c\u7b2c\u4e8c\u306e\u90e8\u4f4d\u3067\u306e\u30ea\u30f3\u9178\u5316\u53cd\u5fdc\u306b\u95a2\u4e0e\u3059\u308b\u4e3b\u8981\u306a\u30ad\u30ca\u30fc\u30bc\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001Akt\u6d3b\u6027\u5316\u306b\u306f\u30a4\u30f3\u30c6\u30b0\u30ea\u30f3\u7d50\u5408\u30ad\u30ca\u30fc\u30bc\uff08Ilk\uff09PDK1\u3001DNA\u4f9d\u5b58\u6027\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\uff08DNA-PK\uff09\u3001\u304a\u3088\u3073\u8840\u7ba1\u62e1\u5f35\u6027\u5931\u8abf\u75c7\u5909\u7570\uff08ATM\uff09\u3068\u3044\u3063\u305f\u4ed6\u306e\u30ad\u30ca\u30fc\u30bc\u3082\u95a2\u4e0e\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059&nbsp;*21&nbsp;\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"293\" height=\"238\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-77-e1748270105940.jpg\" alt=\"\u56f33.7. Akt\u306e\u54fa\u4e73\u985e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\uff1aAkt1\u304a\u3088\u3073Akt2\u306f\u8eab\u4f53\u306e\u5927\u90e8\u5206\u306e\u7d44\u7e54\u306b\u304a\u3044\u3066\u904d\u5728\u7684\u306b\u767a\u73fe\u3059\u308b\u306e\u306b\u5bfe\u3057\u3001Akt3\u306f\u795e\u7d4c\u7d44\u7e54\u304a\u3088\u3073\u7cbe\u5de3\u306b\u304a\u3044\u3066\u9078\u629e\u7684\u306b\u767a\u73fe\u3057\u307e\u3059\u3002Akt1\u304a\u3088\u3073Akt2\u306f\u3055\u307e\u3056\u307e\u306a\u7d44\u7e54\u306b\u767a\u751f\u3059\u308b\u304c\u3093\u306b\u95a2\u9023\u3057\u307e\u3059\u3002\u30e1\u30e9\u30cb\u30f3\u7d30\u80de\u306f\u3001\u795e\u7d4c\u5824\u7d30\u80de\u306b\u7531\u6765\u3057\u3001Akt3\u306f\u30e1\u30e9\u30ce\u30fc\u30de\u3067\u9ad8\u767a\u73fe\u3057\u3066\u3044\u307e\u3059\u3002\" class=\"wp-image-3981\" style=\"width:410px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.7. Akt\u306e\u54fa\u4e73\u985e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0<\/strong><\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">Akt1\u304a\u3088\u3073Akt2\u306f\u8eab\u4f53\u306e\u5927\u90e8\u5206\u306e\u7d44\u7e54\u306b\u304a\u3044\u3066\u904d\u5728\u7684\u306b\u767a\u73fe\u3059\u308b\u306e\u306b\u5bfe\u3057\u3001Akt3\u306f\u795e\u7d4c\u7d44\u7e54\u304a\u3088\u3073\u7cbe\u5de3\u306b\u304a\u3044\u3066\u9078\u629e\u7684\u306b\u767a\u73fe\u3057\u307e\u3059\u3002Akt1\u304a\u3088\u3073Akt2\u306f\u3055\u307e\u3056\u307e\u306a\u7d44\u7e54\u306b\u767a\u751f\u3059\u308b\u304c\u3093\u306b\u95a2\u9023\u3057\u307e\u3059\u3002\u30e1\u30e9\u30cb\u30f3\u7d30\u80de\u306f\u3001\u795e\u7d4c\u5824\u7d30\u80de\u306b\u7531\u6765\u3057\u3001Akt3\u306f\u30e1\u30e9\u30ce\u30fc\u30de\u3067\u9ad8\u767a\u73fe\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/div>\n\n\n\n<p>\u5f53\u793e\u306f\u3001Akt\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u5e45\u5e83\u3044\u6297\u4f53\u304a\u3088\u3073\u8a66\u85ac\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u8a73\u3057\u3044\u60c5\u5831\u306f<a href=\"https:\/\/www.thermofisher.com\/antibody\/primary\/query\/AKT?CID=bid_pca_aup_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3053\u3061\u3089<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002\u8a73\u7d30\u306a\u6280\u8853\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u304a\u3088\u3073\u30ea\u30bd\u30fc\u30b9\u306f\u4ed8\u9332\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#e7e7e7;color:#e7e7e7\" \/>\n\n\n\n<p class=\"has-text-color has-link-color has-medium-font-size wp-elements-454d1e076ee2a13ecfe8c5613c5d09f4\" style=\"color:#ff9400\"><b>AKT1 + AKT2 + AKT3 Rabbit Monoclonal<\/b><br><b>Antibody (E.32.10)<\/b><\/p>\n\n\n\n<p><strong>\u88fd\u54c1\u756a\u53f7 MA5-14999<\/strong><br>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\uff1aWB\u3001IF\u3001IHC (P)\u3001FC\u3001IP\u3001ChIP<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-ad2f72ca wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"384\" height=\"282\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-18-e1748270717156.jpg\" alt=\"AKT1 + AKT2 + AKT3\u306b\u5bfe\u3059\u308b\u6297\u4f53\u3092\u4f7f\u7528\u3057\u3066\u4ee5\u4e0b\u306e\u89e3\u6790\u3092\u5b9f\u65bd\u3002 (A) \u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u89e3\u6790\u306b\u304a\u3051\u308b\u672a\u51e6\u7406Jurkat\u7d30\u80de (\u9752\u8272) \u3068\u975e\u7279\u7570\u7684\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb (\u8d64\u8272) \u306e\u6bd4\u8f03\u3002(B) \u6297pan Akt\u6297\u4f53 (\u7dd1\u8272) \u3092\u4f7f\u7528\u3057\u3066\u7d30\u80de\u67d3\u8272\u3057\u305fHeLa (\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093) \u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f\u8d64\u8272\u86cd\u5149\u30d5\u30a1\u30ed\u30a4\u30b8\u30f3\u3067\u6a19\u8b58\u3002DNA\u306f\u9752\u8272\u86cd\u5149\u8272\u7d20\u3067\u6a19\u8b58\u3002(C) \u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u30d2\u30c8\u30e1\u30e9\u30ce\u30fc\u30de\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3002\" class=\"wp-image-3982\" style=\"width:440px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-18-e1748270717156.jpg 384w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-18-e1748270717156-300x220.jpg 300w\" sizes=\"auto, (max-width: 384px) 100vw, 384px\" \/><\/figure>\n\n\n\n<p class=\"has-small-font-size wp-container-content-1f3f4c55\"><strong>AKT1 + AKT2 + AKT3<\/strong>\u306b\u5bfe\u3059\u308b\u6297\u4f53\u3092\u4f7f\u7528\u3057\u3066\u4ee5\u4e0b\u306e\u89e3\u6790\u3092\u5b9f\u65bd\u3002<br><strong>(A) <\/strong>\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u89e3\u6790\u306b\u304a\u3051\u308b\u672a\u51e6\u7406Jurkat\u7d30\u80de (\u9752\u8272) \u3068\u975e\u7279\u7570\u7684\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb (\u8d64\u8272) \u306e\u6bd4\u8f03\u3002<br><strong>(B) <\/strong> \u6297pan Akt\u6297\u4f53 (\u7dd1\u8272) \u3092\u4f7f\u7528\u3057\u3066\u7d30\u80de\u67d3\u8272\u3057\u305fHeLa (\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093) \u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f\u8d64\u8272\u86cd\u5149\u30d5\u30a1\u30ed\u30a4\u30b8\u30f3\u3067\u6a19\u8b58\u3002DNA\u306f\u9752\u8272\u86cd\u5149\u8272\u7d20\u3067\u6a19\u8b58\u3002(C) \u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u30d2\u30c8\u30e1\u30e9\u30ce\u30fc\u30de\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#e7e7e7;color:#e7e7e7\" \/>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u304c\u3093\u306b\u304a\u3051\u308bakt\u306e\u5f79\u5272\">\u304c\u3093\u306b\u304a\u3051\u308bAkt\u306e\u5f79\u5272<\/h4>\n\n\n\n<p>PTEN\u306e\u6a5f\u80fd\u4e0d\u5168\u306fAkt\u306e\u5236\u5fa1\u4e0d\u80fd\u306a\u6d3b\u6027\u5316\u306b\u5bc6\u63a5\u306b\u95a2\u4fc2\u3057\u3066\u304a\u308a\u3001\u816b\u760d\u7d30\u80de\u5897\u6b96\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002\u6d3b\u6027\u5316\u3055\u308c\u305fAkt\u306f\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3001\u907a\u4f1d\u5b50\u8ee2\u5199\u3001\u7d30\u80de\u5468\u671f\u9032\u884c\u3001\u304a\u3088\u3073\u7d30\u80de\u4ee3\u8b1d\u306e\u5236\u5fa1\u56e0\u5b50\u3092\u542b\u3080\u816b\u760d\u306e\u767a\u751f\u306b\u95a2\u9023\u3059\u308b\u30d7\u30ed\u30bb\u30b9\u3092\u5236\u5fa1\u3059\u308b\u4e0b\u6d41\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u57fa\u8cea\u3092\u6a19\u7684\u3068\u3057\u307e\u3059\u3002Akt\u306e\u57fa\u8cea\u3068\u306a\u308b\u5206\u5b50\u306f50\u7a2e\u985e\u4ee5\u4e0a\u77e5\u3089\u308c\u3066\u304a\u308a\u3001\u7d30\u80de\u5897\u6b96\u306e\u5236\u5fa1\u306b\u95a2\u4e0e\u3059\u308b\u76f4\u63a5\u7684\u306aAkt\u57fa\u8cea\u306b\u306fMDM2\u3001p21\u3001\u304a\u3088\u3073p27\u304c\u542b\u307e\u308c\u307e\u3059\uff08\u56f33.8\uff09<sup>*22&nbsp;<\/sup>\u3002<br>Akt\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306b\u5f71\u97ff\u3059\u308b\u5897\u6b96\u304a\u3088\u3073\u6d3b\u6027\u5316\u5909\u7570\u306f\u3001\u8907\u6570\u306e\u30bf\u30a4\u30d7\u306e\u30d2\u30c8\u304c\u3093\u306b\u304a\u3044\u3066\u540c\u5b9a\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u305d\u306e\u305f\u3081\u3001Akt\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u3001\u306a\u3089\u3073\u306b\u4e0b\u6d41\u7d4c\u8def\u306b\u304a\u3051\u308b\u8abf\u7bc0\u4e0d\u5168\u306f\u591a\u304f\u306e\u304c\u3093\u306e\u30db\u30fc\u30eb\u30de\u30fc\u30af\u306e1\u3064\u3067\u3042\u308a\u3001Akt\u306f\u6297\u304c\u3093\u5264\u304a\u3088\u3073\u6cbb\u7642\u6cd5\u306e\u958b\u767a\u306e\u305f\u3081\u306e\u91cd\u8981\u306a\u6a19\u7684\u3068\u306a\u3063\u3066\u3044\u307e\u3059&nbsp;<sup>*3<\/sup>\u3002<\/p>\n\n\n\n<p>Akt\u5909\u7570\u3092\u6301\u3064\u30d2\u30c8\u304c\u3093\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u9ac4\u819c<\/li>\n\n\n\n<li>\u4e73\u623f<\/li>\n\n\n\n<li>\u5b50\u5bae\u5185\u819c<\/li>\n\n\n\n<li>\u5c3f\u8def<\/li>\n\n\n\n<li>\u7532\u72b6\u817a<\/li>\n\n\n\n<li>\u76ae\u819a<\/li>\n\n\n\n<li>\u80ba<\/li>\n\n\n\n<li>\u5375\u5de3<\/li>\n\n\n\n<li>\u9020\u8840\u5668\u304a\u3088\u3073\u30ea\u30f3\u30d1\u7cfb<\/li>\n\n\n\n<li>\u814e\u81d3<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"378\" height=\"368\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-78-e1750256080814.jpg\" alt=\"\" class=\"wp-image-3983\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-78-e1750256080814.jpg 378w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-78-e1750256080814-300x292.jpg 300w\" sizes=\"auto, (max-width: 378px) 100vw, 378px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.8. Akt\u306e\u5206\u5b50\u6a19\u7684 \u4ee3\u8868\u7684\u306aAkt\u57fa\u8cea\u304a\u3088\u3073\u95a2\u9023\u3059\u308b\u7d30\u80de\u6a5f\u80fd<\/strong><\/p>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#e7e7e7;color:#e7e7e7\" \/>\n\n\n\n<p class=\"has-text-color has-link-color has-medium-font-size wp-elements-454d1e076ee2a13ecfe8c5613c5d09f4\" style=\"color:#ff9400\"><b>AKT1 + AKT2 + AKT3 Rabbit Monoclonal<\/b><br><b>Antibody (E.32.10)<\/b><\/p>\n\n\n\n<p><strong>\u88fd\u54c1\u756a\u53f7 PA5-27237<\/strong><br>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\uff1aIHC (P), IF<\/p>\n\n\n\n<p>E3\u30e6\u30d3\u30ad\u30c1\u30f3\u30bf\u30f3\u30d1\u30af\u8cea\u30ea\u30ac\u30fc\u30bc\u306e\u30de\u30a6\u30b9\u4e8c\u91cd\u5fae\u5c0f\u67d3\u8272\u4f532\u30db\u30e2\u30ed\u30b0 (MDM2) \u306f\u3001MDM2\u907a\u4f1d\u5b50\u306b\u3088\u3063\u3066\u30b3\u30fc\u30c9\u3055\u308c\u3001\u304c\u3093\u907a\u4f1d\u5b50\u3068\u3057\u3066\u6a5f\u80fd\u3057\u307e\u3059\u3002\u3053\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306fAkt\u306e\u76f4\u63a5\u7684\u306a\u57fa\u8cea\u3067\u3001\u7d30\u80de\u5897\u6b96\u3092\u5236\u5fa1\u3057\u307e\u3059\u3002Akt\u306b\u3088\u308bMDM2\u306e\u30ea\u30f3\u9178\u5316\u306f\u3001\u816b\u760d\u6291\u5236\u56e0\u5b50\u907a\u4f1d\u5b50TP53\u306e\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u304a\u3088\u3073\u5206\u89e3\u3092\u8a98\u5c0e\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"380\" height=\"166\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_2-19.jpg\" alt=\"\" class=\"wp-image-21802\" style=\"width:410px;height:auto\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_2-19.jpg 380w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_2-19-300x131.jpg 300w\" sizes=\"auto, (max-width: 380px) 100vw, 380px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\"><strong>MDM2\u306b\u5bfe\u3059\u308b\u6297\u4f53<\/strong>\u3092\u4f7f\u7528\u3057\u305f\u3001<strong>(A) <\/strong>\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3057\u305fA549 (\u30d2\u30c8\u80ba\u304c\u3093) \u7570\u7a2e\u79fb\u690d\u7247\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3002<strong>(B) <\/strong>\u6297MDM2\u6297\u4f53 (\u7dd1\u8272) \u3092\u4f7f\u7528\u3057\u3066\u67d3\u8272\u3057\u305f\u3001\u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u56fa\u5b9aHeLa (\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093) \u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002\u03b1\u30c1\u30e5\u30fc\u30d6\u30ea\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f\u8d64\u8272\u3001\u6838\u306f\u9752\u8272\u3067\u6a19\u8b58\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#e7e7e7;color:#e7e7e7\" \/>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u54fa\u4e73\u985e\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea-mtor\">\u54fa\u4e73\u985e\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\uff08mTOR\uff09<\/h2>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-mtor\u306e\u69cb\u9020-\u6a5f\u80fd\u304a\u3088\u3073\u8abf\u7bc0\">mTOR\u306e\u69cb\u9020\u3001\u6a5f\u80fd\u304a\u3088\u3073\u8abf\u7bc0<\/h4>\n\n\n\n<p>\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u0336\u6297\u771f\u83cc\u6027\u6297\u751f\u7269\u8cea\u3067T\u30ea\u30f3\u30d1\u7403\u6d3b\u6027\u306e\u5f37\u529b\u306a\u963b\u5bb3\u5264\u0336\u306b\u95a2\u3059\u308b\u7814\u7a76\u306f\u3001mTOR\u306e\u767a\u898b\u304a\u3088\u3073\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306e\u89e3\u660e\u306b\u3064\u306a\u304c\u308a\u307e\u3059&nbsp;<sup>*24\u201326<\/sup>\uff08\u56f33.9\u304a\u3088\u3073\u56f33.10\uff09\u3002mTOR\u306f\u3001PI3K\u95a2\u9023\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\uff08PIKK\uff09\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u30e1\u30f3\u30d0\u30fc\u3067\u3001\u8840\u7ba1\u62e1\u5f35\u6027\u5931\u8abf\u75c7\u5909\u7570\uff08ATM\uff09\u3001DNA\u4f9d\u5b58\u6027\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\uff08DNAPK\uff09\u304a\u3088\u3073\u6838\u9178\u306e\u76e3\u8996\u304a\u3088\u3073\u4fee\u5fa9\u30d7\u30ed\u30bb\u30b9\u306b\u95a2\u9023\u3059\u308b\u3044\u304f\u3064\u304b\u306e\u4ed6\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u542b\u307e\u308c\u307e\u3059\u3002mTOR\u306f\u3001\u5897\u6b96\u56e0\u5b50\u7d4c\u8def\u306e\u30b7\u30b0\u30ca\u30eb\u3092\u4f1d\u64ad\u3059\u308b\u6a5f\u80fd\u3092\u6301\u3061\u3001\u305d\u308c\u306b\u3088\u3063\u3066\u7d30\u80de\u306e\u6210\u9577\u3001\u5897\u6b96\u3001\u304a\u3088\u3073\u751f\u5b58\u3092\u30b5\u30dd\u30fc\u30c8\u3057\u307e\u3059\u3002\u3055\u307e\u3056\u307e\u306a\u304c\u3093\u306b\u304a\u3044\u3066\u3001\u4e0a\u65b9\u5236\u5fa1\u3055\u308c\u3066\u3044\u308bmTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u304c\u691c\u51fa\u3055\u308c\u3066\u304a\u308a\u3001mTOR\u3092\u6a19\u7684\u3068\u3059\u308b\u5275\u85ac\u52aa\u529b\u304c\u6210\u529f\u3092\u53ce\u3081\u3066\u3044\u307e\u3059&nbsp;<sup>*27,28<\/sup>\u3002 \u73fe\u5728\u3001\u9032\u884c\u6027\u814e\u7d30\u80de\u304c\u3093\u3001\u306a\u3089\u3073\u306b\u4e73\u623f\u3001\u81b5\u81d3\u3001\u304a\u3088\u3073\u4ed6\u306e\u5668\u5b98\u306e\u7279\u5b9a\u306e\u56fa\u5f62\u304c\u3093\u306b\u5bfe\u3059\u308b\u6cbb\u7642\u85ac\u3068\u3057\u3066\u30012\u3064\u306emTOR\u963b\u5bb3\u5264\u304c\u533b\u85ac\u54c1\u898f\u5236\u6a5f\u95a2\u306b\u627f\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u88683.2\uff09\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"402\" height=\"239\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-79-e1750256813791.jpg\" alt=\"\" class=\"wp-image-3985\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-79-e1750256813791.jpg 402w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-79-e1750256813791-300x178.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-79-e1750256813791-400x239.jpg 400w\" sizes=\"auto, (max-width: 402px) 100vw, 402px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.9. \u30e9\u30d1\u30de\u30a4\u30b7\u30f3 (\u30b7\u30ed\u30ea\u30e0\u30b9) \u306e\u5316\u5b66\u69cb\u9020<\/strong><\/p>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">\u672c\u85ac\u5264\u306f\u3001\u7d30\u83cc\u7a2eStreptomyces hygroscopicus\u304b\u3089\u6700\u521d\u306b\u5358\u96e2\u3055\u308c\u305f\u3001\u30a4\u30f3\u30bf\u30fc\u30ed\u30a4\u30ad\u30f3-2\u304a\u3088\u3073\u514d\u75ab\u53cd\u5fdc\u3092\u5236\u5fa1\u3059\u308b\u4ed6\u306e\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u306e\u5f37\u529b\u306a\u963b\u5bb3\u5264\u3067\u3059\u3002\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u306f\u3001\u79fb\u690d\u7247\u62d2\u7d76\u53cd\u5fdc\u3092\u6291\u5236\u3059\u308b\u305f\u3081\u306b\u983b\u7e41\u306b\u51e6\u65b9\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/div>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"410\" height=\"131\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-20-e1750256946540.jpg\" alt=\"\" class=\"wp-image-3986\" style=\"width:600px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-20-e1750256946540.jpg 410w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-20-e1750256946540-300x96.jpg 300w\" sizes=\"auto, (max-width: 410px) 100vw, 410px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.10. mTOR\u306e\u69cb\u9020<\/strong><\/p>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">\u8907\u6570\u306e\u30c9\u30e1\u30a4\u30f3\u304b\u3089\u306a\u308b\u5de8\u5927\u30bf\u30f3\u30d1\u30af\u8cea (2,549 aa\u3001~250 kD) \u3067\u3001N\u672b\u7aef\u30c9\u30e1\u30a4\u30f3\u306f\u8907\u6570\u306eHEAT\u30ea\u30d4\u30fc\u30c8 (<strong>H<\/strong>untingtin\u3001<strong>e<\/strong>longation factor 3\u3001PP2<strong>A<\/strong>\u3001\u304a\u3088\u3073<strong>T<\/strong>OR1\u306e\u982d\u6587\u5b57\u306b\u7531\u6765) \u304b\u3089\u69cb\u6210\u3055\u308c\u307e\u3059\u3002mTOR\u306f\u3001Raptor (regulatory-associated protein with TOR) \u3084Rictor (rapamycin-insensitive companion of TOR) \u3068\u76f8\u4e92\u4f5c\u7528\u3057\u3001\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u307e\u3059\u3002mTOR\u89e6\u5a92\u6a5f\u80fd\u306e\u6d3b\u6027\u5316\u306b\u306f\u30012\u3064\u306e<strong>F<\/strong>RAP (FKBP12-\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea)\/TOR)\u3001<strong>A<\/strong>TM (\u6bdb\u7d30\u8840\u7ba1\u62e1\u5f35\u5931\u8abf\u75c7\u5909\u7570)\u3001<strong>T<\/strong>RRAP (\u30c8\u30e9\u30f3\u30b9\u6d3b\u6027\/\u5f62\u8cea\u8ee2\u63db\u30c9\u30e1\u30a4\u30f3\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea) (FAT) \u30c9\u30e1\u30a4\u30f3\u304c\u5fc5\u8981\u3067\u3001C\u672b\u7aef\u5074\u306b\u3042\u308bFAT\u30c9\u30e1\u30a4\u30f3\u304cFATC\u3067\u3059\u3002FKBP12-\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u7d50\u5408 (FRB) \u30c9\u30e1\u30a4\u30f3\u306b\u306f\u3001FKBP12-\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u8907\u5408\u4f53\u304c\u7d50\u5408\u3059\u308b\u4ed6\u306b\u3001Raptor\u3092\u542b\u3080mTORC\u30e1\u30f3\u30d0\u30fc\u3082\u76f8\u4e92\u4f5c\u7528\u3057\u307e\u3059\u3002\u30ad\u30ca\u30fc\u30bc (KIN) \u30c9\u30e1\u30a4\u30f3\u304a\u3088\u3073\u95a2\u9023NRD\u9818\u57df\u306b\u306f\u30bb\u30ea\u30f3\/\u30b9\u30ec\u30aa\u30cb\u30f3\u6d3b\u6027\u304c\u5b58\u5728\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"596\" height=\"299\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/e1837f6edeaa584356971995b55ba2f8.jpg\" alt=\"\" class=\"wp-image-21808\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/e1837f6edeaa584356971995b55ba2f8.jpg 596w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/e1837f6edeaa584356971995b55ba2f8-300x151.jpg 300w\" sizes=\"auto, (max-width: 596px) 100vw, 596px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.11. \u30bf\u30f3\u30d1\u30af\u8ceaRaptor\u304a\u3088\u3073Rictor\u306f\u3001\u305d\u308c\u305e\u308c\u8907\u5408\u4f53mTORC1\u304a\u3088\u3073mTORC2\u306e\u30b3\u30a2\u30b3\u30f3\u30dd\u30fc\u30cd\u30f3\u30c8\u3067\u3001mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306b\u4e0d\u53ef\u6b20\u3067\u3059\u3002<\/strong><\/p>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#e7e7e7;color:#e7e7e7\" \/>\n\n\n\n<p>\u5f53\u793e\u306f\u3001Raptor\u3001Rictor \u304a\u3088\u3073mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306e\u30e1\u30c7\u30a3\u30a8\u30fc\u30bf\u30fc\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u4ed6\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u5e45\u5e83\u3044\u6297\u4f53\u304a\u3088\u3073\u8a66\u85ac\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u8a73\u3057\u3044\u60c5\u5831\u306f<strong><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/antibodies.html\" target=\"_blank\" rel=\"noreferrer noopener\">\u3053\u3061\u3089<\/a><\/strong>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"has-text-color has-link-color has-medium-font-size wp-elements-c7ccd573653f385bbf82da130c385cbe\" style=\"color:#ff9400\"><strong>RAPTOR Rabbit Polyclonal Antibody<\/strong><\/p>\n\n\n\n<p><strong>\u88fd\u54c1\u756a\u53f7 PA1-41269<br>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\uff1aIHC\u3001WB<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"350\" height=\"170\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_RAPTOR-Rabbit-Polyclonal-Antibody.jpg\" alt=\"\" class=\"wp-image-21814\" style=\"width:auto;height:170px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_RAPTOR-Rabbit-Polyclonal-Antibody.jpg 350w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_RAPTOR-Rabbit-Polyclonal-Antibody-300x146.jpg 300w\" sizes=\"auto, (max-width: 350px) 100vw, 350px\" \/><\/figure>\n\n\n\n<p class=\"has-small-font-size\"><strong>Raptor\u306b\u5bfe\u3059\u308b\u6297\u4f53<\/strong>\u3092\u4f7f\u7528\u3057\u3001(A) \u30d2\u30c8\u5c0f\u8178\u7d44\u7e54\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u306a\u3089\u3073\u306b (B) \uff08a\uff09HEK293 (\u4e0d\u6b7b\u5316\u30d2\u30c8\u814e\u81d3\u764c) \u304a\u3088\u3073 (b) NIH\/3T3 (\u4e0d\u6b7b\u5316\u30de\u30a6\u30b9\u7dda\u7dad\u82bd\u7d30\u80de) \u306e\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u306b\u304a\u3051\u308bRaptor\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u691c\u51fa\u3002<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"has-text-color has-link-color has-medium-font-size wp-elements-490125d725440f8ad3e3aa17c19b843b\" style=\"color:#ff9400\"><strong>RICTOR Goat Polyclonal Antibody<\/strong><\/p>\n\n\n\n<p><strong>\u88fd\u54c1\u756a\u53f7 PA5-18370<br>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\uff1aIHC<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"214\" height=\"169\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/cancer8_RICTOR-Goat-Polyclonal-Antibody.jpg\" alt=\"\" class=\"wp-image-21815\" style=\"width:auto;height:170px\" \/><\/figure>\n\n\n\n<p class=\"has-small-font-size\"><strong>Rictor\u306b\u5bfe\u3059\u308b\u6297\u4f53<\/strong>\u3092\u4f7f\u7528\u3057\u3001\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3057\u305f\u30d2\u30c8\u814e\u81d3\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3002<\/p>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#e7e7e7;color:#e7e7e7\" \/>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-mtor\u306e\u6a5f\u80fd\u304a\u3088\u3073\u5236\u5fa1\">mTOR\u306e\u6a5f\u80fd\u304a\u3088\u3073\u5236\u5fa1<\/h4>\n\n\n\n<p>PI3K\/Akt\u7d4c\u8def\u306e\u4e0b\u6d41\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u3067\u3042\u308bmTOR\u306f\u3001\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u3001\u8907\u6570\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u3088\u3063\u3066\u6d3b\u6027\u5316\u3055\u308c\u307e\u3059\u3002mTOR\u306f\u3001mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306e\u7a2e\u3005\u306e\u5074\u9762\u3092\u5236\u5fa1\u3059\u308b\u3055\u307e\u3056\u307e\u306a\u8abf\u7bc0\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u3068\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u307e\u3059\uff08\u56f33.9\u304a\u3088\u30733.10\uff09\u3002mTOR\u306f\u30012\u7a2e\u985e\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u8907\u5408\u4f53mTORC1\u304a\u3088\u3073mTORC2\u306e\u30b3\u30a2\u89e6\u5a92\u30e6\u30cb\u30c3\u30c8\u3067\u3059\u3002mTORC1\u8907\u5408\u4f53\u306f\u3001\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u611f\u53d7\u6027\u3067\u3001mTOR\u3001Raptor\u3001\u304a\u3088\u3073mLST8\u304b\u3089\u69cb\u6210\u3055\u308c\u307e\u3059\u3002mTORC1\u306f\u3001\u7ffb\u8a33\u6291\u5236\u56e0\u5b504E-BP1\u304a\u3088\u3073\u30ea\u30dc\u30bd\u30fc\u30e0\u30bf\u30f3\u30d1\u30af\u8ceaS6\u30ad\u30ca\u30fc\u30bc\uff08S6K\uff09\u306e\u4e21\u65b9\u3092\u30ea\u30f3\u9178\u5316\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u306e\u6210\u9577\u304a\u3088\u3073\u5897\u6b96\u3092\u5236\u5fa1\u3057\u307e\u3059\u3002\u7ffb\u8a33\u3001\u30ea\u30dc\u30bd\u30fc\u30e0\u751f\u5408\u6210\u3001\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u3001\u30b0\u30eb\u30b3\u30fc\u30b9\u4ee3\u8b1d\u3001\u4f4e\u9178\u7d20\u306b\u5bfe\u3059\u308b\u7d30\u80de\u53cd\u5fdc\u3001\u304a\u3088\u3073\u8ee2\u79fb\u306a\u3069\u306e\u3055\u307e\u3056\u307e\u306a\u816b\u760d\u7d30\u80de\u7279\u7570\u7684\u306a\u30d7\u30ed\u30bb\u30b9\u3092\u542b\u3080\u305d\u306e\u4ed6\u306e\u751f\u7269\u5b66\u7684\u30d7\u30ed\u30bb\u30b9\u3082mTORC1\u306b\u3088\u3063\u3066\u5236\u5fa1\u3055\u308c\u307e\u3059&nbsp;<sup>*30<\/sup>\u3002<\/p>\n\n\n\n<p>mTORC2\u8907\u5408\u4f53\u306f\u3001\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u62b5\u6297\u6027\u3067\u3001mTOR\u3001Rictor\u3001mLST8\u3001\u304a\u3088\u3073mSin1\u304b\u3089\u69cb\u6210\u3055\u308c\u307e\u3059\u3002mTORC2\u8907\u5408\u4f53\u306f\u3001\u8907\u6570\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u0336S473\u4e0a\u306e\u30bb\u30ea\u30f3\u304a\u3088\u3073\u30b9\u30ec\u30aa\u30cb\u30f3\u30ad\u30ca\u30fc\u30bcAkt\u3001\u8840\u6e05\u30b0\u30eb\u30b3\u30b3\u30eb\u30c1\u30b3\u30a4\u30c9\u8abf\u7bc0\u30ad\u30ca\u30fc\u30bc1\uff08SGK1\uff09\u3001\u306a\u3089\u3073\u306b\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bcC \u03b1\uff08PKC\u03b1\uff09\u0336\u3092\u30ea\u30f3\u9178\u5316\u3057\u307e\u3059\u3002mTORC2\u306f\u3001\u7d30\u80de\u306e\u751f\u5b58\u304a\u3088\u3073\u5897\u6b96\u3092\u5236\u5fa1\u3059\u308b\u305f\u3081\u306b\u6a5f\u80fd\u3057\u307e\u3059\uff08\u56f33.12\uff09<em>27\u3002 \u3053\u308c\u3089\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306b\u3064\u3044\u3066\u8a73\u7d30\u306b\u7406\u89e3\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u816b\u760d\u9032\u884c\u306b\u95a2\u9023\u3059\u308b\u30ad\u30fc\u30e1\u30c7\u30a3\u30a8\u30fc\u30bf\u30fc\u3092\u6a19\u7684\u3068\u3057\u3066\u963b\u5bb3\u3059\u308b\u85ac\u5264\u306e\u5408\u7406\u7684\u306a\u533b\u85ac\u54c1\u958b\u767a\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f<\/em>31 \u3002\u4f8b\u3048\u3070\u3001VEGF\u3001PDGFR\u3001EGF\u3001\u304a\u3088\u3073PI3K\/Akt\/mTOR\u7d4c\u8def\u3092\u6d3b\u6027\u5316\u3059\u308b\u591a\u304f\u306e\u4ed6\u306e\u7d4c\u8def\u3092\u4ecb\u3059\u308b\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3092\u5236\u9650\u3059\u308b\u30c1\u30ed\u30b7\u30f3\u30ad\u30ca\u30fc\u30bc\u53d7\u5bb9\u4f53\u963b\u5bb3\u5264\u304c\u958b\u767a\u3055\u308c\u3066\u304d\u307e\u3057\u305f\uff08\u88682.1\uff09\u3002\u540c\u69d8\u306b\u3001mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3092\u76f4\u63a5\u963b\u5bb3\u3059\u308b\u85ac\u5264\u304c\u814e\u7d30\u80de\u304c\u3093\u304a\u3088\u3073\u4ed6\u306e\u56fa\u5f62\u816b\u760d\u60a3\u8005\u306b\u5bfe\u3059\u308b\u6cbb\u7642\u85ac\u3068\u3057\u3066\u958b\u767a\u3055\u308c\u3001\u627f\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aca252f94bdc&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aca252f94bdc\" class=\"wp-block-image aligncenter size-full is-resized wp-lightbox-container\"><img loading=\"lazy\" decoding=\"async\" width=\"402\" height=\"514\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-81-e1750258260378.jpg\" alt=\"\" class=\"wp-image-3988\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-81-e1750258260378.jpg 402w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-81-e1750258260378-235x300.jpg 235w\" sizes=\"auto, (max-width: 402px) 100vw, 402px\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"Enlarge\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.12. mTOR\u8907\u5408\u4f53\u306emTORC1\u304a\u3088\u3073mTORC2\u306f\u3001\u7d30\u80de\u7dad\u6301\u306e\u5927\u304d\u304f\u7570\u306a\u308b\u5074\u9762\u3092\u8abf\u7bc0<\/strong><\/p>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">\u764c\u5897\u6b96\u56e0\u5b50\u3001PTEN\u55aa\u5931\u3001\u307e\u305f\u306f\u4f53\u7d30\u80de\u5909\u7570\u306b\u3088\u3063\u3066\u6d3b\u6027\u5316\u3057\u305fPI3K\u306f\u3001Akt\u304a\u3088\u3073mTORC\u8907\u5408\u4f53\u306e\u6d3b\u6027\u5316\u306b\u5bc4\u4e0e\u3057\u307e\u3059\u3002<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"319\" height=\"162\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-21-e1750258454223.jpg\" alt=\"\" class=\"wp-image-3989\" style=\"width:410px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-21-e1750258454223.jpg 319w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-21-e1750258454223-300x152.jpg 300w\" sizes=\"auto, (max-width: 319px) 100vw, 319px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>mTOR Rabbit Polyclonal Antibody<\/strong><\/p>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\"><strong>(\u5de6) <\/strong>\u6297\u4f53\u4e0d\u4f7f\u7528\u306eHeLa (\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093) \u7d30\u80de\u306e\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u304a\u3088\u3073<strong> (\u53f3) <\/strong>\u6297mTOR\u6297\u4f53 (\u88fd\u54c1\u756a\u53f7 PA1-518) \u3092\u4f7f\u7528\u3057\u305fHeLa\u7d30\u80de\u306e\u514d\u75ab\u86cd\u5149\u5206\u6790\u3002F-\u30a2\u30af\u30c1\u30f3 (\u8d64\u8272) \u304a\u3088\u3073\u6838 (\u9752\u8272) \u3082\u67d3\u8272\u3002<\/p>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u304c\u3093\u306b\u304a\u3051\u308bmtor\u306e\u5f79\u5272\">\u304c\u3093\u306b\u304a\u3051\u308bmTOR\u306e\u5f79\u5272<\/h2>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-mtor\u306e\u963b\u5bb3\">mTOR\u306e\u963b\u5bb3<\/h4>\n\n\n\n<p>\u5897\u6b96\u56e0\u5b50\u7d4c\u8def\u306fmTOR\u306b\u3088\u3063\u3066\u6d3b\u6027\u5316\u3055\u308c\u308b\u4ed6\u306b\u3001\u4f4e\u9178\u7d20\u72b6\u614b\u304a\u3088\u3073\u30a2\u30df\u30ce\u9178\u306e\u4f4e\u30ec\u30d9\u30eb\u72b6\u614b\u306b\u3088\u3063\u3066\u958b\u59cb\u3055\u308c\u307e\u3059\uff08\u56f33.12\uff09<sup>*32,33<\/sup> \u3002\u4f4e\u9178\u7d20\u72b6\u614b\u306e\u6301\u7d9a\u306f\u751f\u547d\u306b\u95a2\u308f\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u3001\u304c\u3093\u7d30\u80de\u306f\u9178\u7d20\u6b63\u5e38\u72b6\u614b\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306e\u751f\u5b58\u6226\u7565\u306b\u5bfe\u5fdc\u3057\u3066\u3044\u307e\u3059\u3002\u8ee2\u5199\u56e0\u5b50\u306e\u4f4e\u9178\u7d20\u8a98\u5c0e\u56e0\u5b501\u03b1\uff08HIF-1\u03b1)\u306f\u3001\u8840\u7ba1\u65b0\u751f\u306e\u4e3b\u8981\u306a\u5236\u5fa1\u56e0\u5b50\u3067\u3001\u4f4e\u9178\u7d20\u306b\u5bfe\u3059\u308b\u7d30\u80de\u53cd\u5fdc\u306b\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002PI3K\/Akt\/mTOR\u7d4c\u8def\u306f\u3001HIF-1\u907a\u4f1d\u5b50\u767a\u73fe\u30ec\u30d9\u30eb\u3092\u5897\u5927\u3055\u305b\u3001\u4f4e\u9178\u7d20\u6761\u4ef6\u4e0b\u3067\u306e\u816b\u760d\u7d30\u80de\u5897\u6b96\u306b\u5bfe\u3059\u308b\u751f\u5b58\u30b7\u30b0\u30ca\u30eb\u3092\u4f1d\u9054\u3057\u307e\u3059\u3002VHL\u907a\u4f1d\u5b50\u306f\u3001HIF-1\u03b1\u306e\u5206\u89e3\u306b\u5fc5\u8981\u3068\u3055\u308c\u308b\u30d5\u30a9\u30f3\u30d2\u30c3\u30da\u30eb\u30fb\u30ea\u30f3\u30c9\u30a6\u816b\u760d\u6291\u5236\u56e0\u5b50\u3092\u30b3\u30fc\u30c9\u3057\u307e\u3059\u3002\u4f4e\u9178\u7d20\u6761\u4ef6\u4e0b\u306b\u304a\u3044\u3066\u3001HIF-1\u03b1\u306fVEGF\u3001PDGF\u3001\u304a\u3088\u3073\u8840\u7ba1\u65b0\u751f\u3092\u5a92\u4ecb\u3059\u308b\u4ed6\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5236\u5fa1\u3059\u308b\u907a\u4f1d\u5b50\u306e\u8ee2\u5199\u3092\u4fc3\u9032\u3057\u307e\u3059\u3002HIF-1\u03b1\u3001mTOR\u3001\u304a\u3088\u3073\u95a2\u9023\u3059\u308b\u8abf\u7bc0\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306f\u3001\u816b\u760d\u7d30\u80de\u304c\u4f4e\u9178\u7d20\u72b6\u614b\u306b\u5bfe\u5fdc\u3057\u3001\u514b\u670d\u3059\u308b\u3053\u3068\u3092\u53ef\u80fd\u3068\u3057\u307e\u3059&nbsp;<sup>*34<\/sup> \u3002\u4f4e\u9178\u7d20\u306f\u8840\u7ba1\u65b0\u751f\u306e\u5f37\u529b\u306a\u30a4\u30f3\u30c7\u30e5\u30fc\u30b5\u30fc\u3067\u3042\u308b\u305f\u3081\u3001HIF-1\u03b1\u306e\u5236\u5fa1\u56e0\u5b50\u3067\u3042\u308bTOR\u306f\u533b\u85ac\u54c1\u958b\u767a\u306e\u6a19\u7684\u3068\u306a\u308a\u307e\u3059\u3002\u73fe\u5728\u30012\u7a2e\u985e\u306emTOR\u963b\u5bb3\u5264\u0336\u3044\u305a\u308c\u3082apalogs\uff08\u30e9\u30d1\u30de\u30a4\u30b7\u30f3\u306e\u30a2\u30ca\u30ed\u30b0\uff09\u0336\u304c\u56fa\u5f62\u816b\u760d\u306b\u5bfe\u3059\u308b\u6297\u8840\u7ba1\u65b0\u751f\u7642\u6cd5\u3078\u306e\u4f7f\u7528\u306b\u3064\u3044\u3066\u627f\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u88683.2\uff09<sup>*28&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aca252f95893&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aca252f95893\" class=\"wp-block-image aligncenter size-full wp-lightbox-container\"><img loading=\"lazy\" decoding=\"async\" width=\"609\" height=\"460\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-82-e1750258643382.jpg\" alt=\"mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30ab\u30b9\u30b1\u30fc\u30c9\" class=\"wp-image-3990\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-82-e1750258643382.jpg 609w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-82-e1750258643382-300x227.jpg 300w\" sizes=\"auto, (max-width: 609px) 100vw, 609px\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"Enlarge\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.13. mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u30ab\u30b9\u30b1\u30fc\u30c9<\/strong><\/p>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\">PI3K\/Akt\/mTOR\u7d4c\u8def\u306e\u5897\u6b96\u56e0\u5b50\u8a98\u5c0e\u6027\u30a2\u30fc\u30e0\u3092\u4ecb\u3059\u308b\u30b7\u30b0\u30ca\u30eb\u4f1d\u64ad\u306f\u3001\u6700\u7d42\u7684\u306b\u7d30\u80de\u5897\u6b96\u3092\u8a98\u5c0e\u3057\u307e\u3059\u3002\u4f4e\u9178\u7d20\u307e\u305f\u306f\u4f4e\u6804\u990a\u6761\u4ef6\u4e0b\u3067\u3001\u672c\u7d4c\u8def\u306fVHL\u3001HIF-1\u3001\u304a\u3088\u3073\u5e83\u7bc4\u306amTOR\u7d4c\u8def\u30e1\u30c7\u30a3\u30a8\u30fc\u30bf\u30fc\u306e\u5354\u8abf\u7684\u5236\u5fa1\u3092\u4ecb\u3057\u3066\u8840\u7ba1\u65b0\u751f\u30d7\u30ed\u30bb\u30b9\u3092\u6d3b\u6027\u5316\u3057\u307e\u3059\u3002\u8840\u7ba1\u65b0\u751f\u963b\u5bb3\u5264\u306f\u3001mTOR\u3001VEGF\u3001PDGF\u3001\u304a\u3088\u3073\u816b\u760d\u95a2\u9023\u8840\u7ba1\u5f62\u6210\u306b\u95a2\u4e0e\u3059\u308b\u4ed6\u306e\u5897\u6b96\u56e0\u5b50\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u3092\u59a8\u5bb3\u3057\u307e\u3059\u3002mTOR\u963b\u5bb3\u5264\u306f\u3001\u8840\u7ba1\u65b0\u751f\u963b\u5bb3\u52b9\u679c\u306e\u4ed6\u306b\u3001\u816b\u760d\u7d30\u80de\u306b\u304a\u3051\u308b\u76f4\u63a5\u7684\u306a\u6297\u5897\u6b96\u52b9\u679c\u3092\u6301\u3064\u53ef\u80fd\u6027\u304c\u524d\u81e8\u5e8a\u8a66\u9a13\u306b\u304a\u3044\u3066\u793a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/div>\n\n\n\n<p>\u30d5\u30a9\u30f3\u30d2\u30c3\u30da\u30eb\u30fb\u30ea\u30f3\u30c9\u30a6\u75c7\u5019\u7fa4\u306f\u3001VHL\u907a\u4f1d\u5b50\u306b\u304a\u3051\u308b\u751f\u6b96\u7d30\u80de\u7cfb\u5217\u5909\u7570\u306b\u3088\u3063\u3066\u5f15\u304d\u8d77\u3053\u3055\u308c\u308b\u5bb6\u65cf\u6027\u304c\u3093\u75c7\u5019\u7fa4\u3067\u3059\u3002\u672c\u75c7\u5019\u7fa4\u306f\u3001\u7279\u5b9a\u306e\u30bf\u30a4\u30d7\u306e\u60aa\u6027\u816b\u760d\u304a\u3088\u3073\u826f\u6027\u816b\u760d\u3092\u767a\u73fe\u3059\u308b\u30ea\u30b9\u30af\u3092\u5897\u52a0\u3055\u305b\u307e\u3059\u3002VHL\u306e\u5b64\u767a\u6027\u5909\u7570\u306f\u3001\u814e\u81d3\u306e\u3055\u307e\u3056\u307e\u306a\u304c\u3093\u306b\u3082\u95a2\u9023\u3057\u307e\u3059&nbsp;<sup>*37<\/sup> \u3002\u3053\u306e\u907a\u4f1d\u5b50\u306b\u30b3\u30fc\u30c9\u3055\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u30e6\u30d3\u30ad\u30c1\u30f3\u30ea\u30ac\u30fc\u30bcE3\u6d3b\u6027\u3092\u6301\u3061\u3001\u30a8\u30ed\u30f3\u30ae\u30f3B\u3001\u30a8\u30ed\u30f3\u30ae\u30f3C\u3001\u304a\u3088\u3073\u30ab\u30ea\u30f3-2\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u542b\u3080\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u307e\u3059\u3002VHL\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u4f4e\u9178\u7d20\u72b6\u614b\u3067\u751f\u3058\u308b\u7279\u5b9a\u306e\u7d30\u80de\u53cd\u5fdc\u3092\u5236\u5fa1\u3059\u308b\u8ee2\u5199\u56e0\u5b50\u3067\u3042\u308b\u4f4e\u9178\u7d20\u8a98\u5c0e\u56e0\u5b50\uff08HIF\uff09\u306e\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u304a\u3088\u3073\u5206\u89e3\u306b\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059&nbsp;<sup>*38&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-horizontal is-content-justification-left is-nowrap is-layout-flex wp-container-core-group-is-layout-e0598304 wp-block-group-is-layout-flex\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"296\" height=\"167\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/2-22-e1750258801323.jpg\" alt=\"VHL\u30a6\u30b5\u30ae\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\" class=\"wp-image-3991\" style=\"width:416px;height:auto\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<p class=\"has-small-font-size\"><strong>VHL\u30a6\u30b5\u30ae\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53<\/strong><br>\u6297VHL\u6297\u4f53 (\u88fd\u54c1\u756a\u53f7 PA5-27322) \u3092\u4f7f\u7528\u3057\u305f\u3001MOLT4 (\u4e0d\u6b7b\u5316\u30d2\u30c8\u6025\u6027\u30ea\u30f3\u30d1\u82bd\u7403\u6027\u767d\u8840\u75c5) \u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\u3002(\u5de6) \u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3057\u305f\u30d2\u30c8\u7d50\u8178\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5206\u6790\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<p class=\"has-text-align-center\"><strong>\u88683.2. \u60aa\u6027\u816b\u760d\u6cbb\u7642\u85ac\u3068\u3057\u3066\u7c73\u56fdFDA\u3067\u627f\u8a8d\u3055\u308c\u305fmTOP\u963b\u5bb3\u5264<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"401\" height=\"207\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-83-e1750260849508.jpg\" alt=\"\u88683.2\uff0e\u60aa\u6027\u816b\u760d\u6cbb\u7642\u85ac\u3068\u3057\u3066\u7c73\u56fdFDA\u3067\u627f\u8a8d\u3055\u308c\u305fmTOP\u963b\u5bb3\u5264\" class=\"wp-image-3992\" style=\"width:520px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-83-e1750260849508.jpg 401w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-83-e1750260849508-300x155.jpg 300w\" sizes=\"auto, (max-width: 401px) 100vw, 401px\" \/><\/figure>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"407\" height=\"519\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/f9ce1fe778a1d0d62768e718a950e332.jpg\" alt=\"\u56f33.14. \u30c6\u30e0\u30b7\u30ed\u30ea\u30e0\u30b9\u304a\u3088\u3073\u30a8\u30d9\u30ed\u30ea\u30e0\u30b9\u306e\u79d1\u5b66\u69cb\u9020\" class=\"wp-image-21849\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/f9ce1fe778a1d0d62768e718a950e332.jpg 407w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/01\/f9ce1fe778a1d0d62768e718a950e332-235x300.jpg 235w\" sizes=\"auto, (max-width: 407px) 100vw, 407px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33.14. \u30c6\u30e0\u30b7\u30ed\u30ea\u30e0\u30b9\u304a\u3088\u3073\u30a8\u30d9\u30ed\u30ea\u30e0\u30b9\u306e\u79d1\u5b66\u69cb\u9020<\/strong><\/p>\n\n\n\n<p>mTOR\u963b\u5bb3\u5264\u304c\u304c\u3093\u6cbb\u7642\u85ac\u3068\u3057\u3066\u73fe\u5728\u627f\u8a8d\u3055\u308c\u3066\u3044\u308b\u90e8\u4f4d\u306e\u4ed6\u306b\u3082\u3001\u4ed6\u306e\u8eab\u4f53\u9818\u57df\u306e\u7d44\u7e54\u306b\u304a\u3044\u3066mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u5909\u7570\u304c\u691c\u51fa\u3055\u308c\u3066\u3044\u307e\u3059\u00a0<sup>*3\u00a0<\/sup>\u3002<\/p>\n\n\n\n<p>mTOR\u5909\u7570\u3092\u6301\u3064\u30d2\u30c8\u304c\u3093\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5b50\u5bae\u5185\u819c<\/li>\n\n\n\n<li>\u814e\u81d3<\/li>\n\n\n\n<li>\u7d50\u8178\u76f4\u8178<\/li>\n\n\n\n<li>\u80ba<\/li>\n\n\n\n<li>\u76ae\u819a<\/li>\n\n\n\n<li>\u98df\u9053<\/li>\n\n\n\n<li>\u4e0a\u6c17\u9053<\/li>\n\n\n\n<li>\u5c3f\u8def<\/li>\n\n\n\n<li>\u4e73\u623f<\/li>\n\n\n\n<li>\u5375\u5de3<\/li>\n\n\n\n<li>\u9020\u8840\u5668\u304a\u3088\u3073\u30ea\u30f3\u30d1\u7cfb<\/li>\n\n\n\n<li>\u809d\u81d3<\/li>\n\n\n\n<li>\u81b5\u81d3<\/li>\n\n\n\n<li>\u8133<\/li>\n<\/ul>\n\n\n\n<p>PI3K\/Akt\/mTOR\u8abf\u7bc0\u3068\u4ed6\u306e\u7d4c\u8def\u9593\u306e\u30af\u30ed\u30b9\u30c8\u30fc\u30af\u3092\u5236\u5fa1\u3059\u308b\u30d7\u30ed\u30bb\u30b9\u3092\u89e3\u660e\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u533b\u85ac\u54c1\u898f\u5236\u5f53\u5c40\u306b\u627f\u8a8d\u3055\u308c\u308b\u3001\u3055\u307e\u3056\u307e\u306a\u304c\u3093\u3092\u6cbb\u7642\u6a19\u7684\u3068\u3057\u305fmTOR\u963b\u5bb3\u5264\u3092\u5408\u7406\u7684\u306b\u958b\u767a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u7b2c\u4e00\u4e16\u4ee3\u306emTOR\u963b\u5bb3\u5264\u306f\u3001\u7279\u5b9a\u306e\u6761\u4ef6\u4e0b\u3067\u6709\u52b9\u6027\u3092\u793a\u3057\u307e\u3059\u304c\u3001\u4e3b\u3068\u3057\u3066mTORC1\u5a92\u4ecb\u6027\u30d7\u30ed\u30bb\u30b9\u3092\u963b\u5bb3\u3057\u307e\u3059\u3002\u4e21\u65b9\u306emTORC\u8907\u5408\u4f53\u3092\u6a19\u7684\u3068\u3059\u308b\u7b2c\u4e8c\u4e16\u4ee3\u306e\u963b\u5bb3\u5264\u306f\u3001\u958b\u767a\u4e2d\u3067\u3001 \u816b\u760d\u8a98\u5c0e\u6027Akt\/mTOR\u30d7\u30ed\u30bb\u30b9\u3092\u30d6\u30ed\u30c3\u30af\u3059\u308b\u52b9\u679c\u304c\u3088\u308a\u9ad8\u3044\u3068\u8003\u3048\u3089\u308c\u307e\u3059&nbsp;<sup>*39&nbsp;<\/sup>\u3002<\/p>\n\n\n\n<p>\u5f53\u793e\u306f\u3001mTOR\u7d4c\u8def\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u5e45\u5e83\u3044\u6297\u4f53\u304a\u3088\u3073\u8a66\u85ac\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u8a73\u3057\u3044\u60c5\u5831\u306f<strong><a href=\"http:\/\/www.thermofisher.com\/mTOR-Signaling?CID=bid_pca_aup_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab13_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">\u3053\u3061\u3089<\/a><\/strong>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#e7e7e7;color:#e7e7e7\" \/>\n\n\n\n<p class=\"has-text-color has-link-color has-medium-font-size wp-elements-501138a9949c77d22a600cf763ec878a\" style=\"color:#ff9400\"><strong>mTOR Rabbit Monoclonal Antibody (7H13L18) ABfinity Recombinant<\/strong><\/p>\n\n\n\n<p><strong>\u88fd\u54c1\u756a\u53f7 701483<br>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\uff1aWB\u3001ICC\u3001FC<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"727\" height=\"224\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-84-e1750261545761.jpg\" alt=\"mTOR\u306b\u5bfe\u3059\u308b\u6297\u4f53\u3092\u4f7f\u7528\u3057\u305f\u3001(A) HEK293 (\u4e0d\u6b7b\u5316\u30d2\u30c8\u80da\u814e\u81d3) \u7d30\u80de\u62bd\u51fa\u7269\u306b\u304a\u3051\u308bmTOR\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d7\u30ed\u30c3\u30c8\u89e3\u6790\u3001(B) HeLa (\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093) \u7d30\u80de\u306b\u304a\u3051\u308bmTOR\u306e\u514d\u75ab\u7d30\u80de\u5316\u5b66\u5206\u6790\u306b\u304a\u3044\u3066\u3001\u7d30\u80de\u3092\u3055\u3089\u306bAlexa Fluor\u2122 488\u6a19\u8b58\u30e4\u30ae\u6297\u30a6\u30b5\u30ae\u4e8c\u6b21\u6297\u4f53\u3067\u67d3\u8272\u3057\u3066\u691c\u51fa(a)\uff1a\u6838\u3092DAPI\u3067\u67d3\u8272(b)\uff1a\u30a2\u30af\u30c1\u30f3\u3092Alexa Fluor\u2122 594\u30d5\u30a1\u30ed\u30a4\u30b8\u30f3\u3067\u67d3\u8272(c)\uff1a\u7d30\u80de\u8cea\u304a\u3088\u3073\u6838\u5c40\u5728\u3092\u793a\u3059\u91cd\u306d\u5408\u308f\u305b\u753b\u50cf(d)\u3001(C) \u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u89e3\u6790\u306b\u304a\u3051\u308b\u6297mTOR\u6297\u4f53\u6a19\u8b58HeLa\u7d30\u80de (\u5857\u308a\u3064\u3076\u3057) \u3068\u975e\u67d3\u8272\u7d30\u80de (\u9752) \u304a\u3088\u3073\u30a2\u30a4\u30bd\u30bf\u30a4\u30d7\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb (\u9ed2) \u3068\u306e\u6bd4\u8f03\u3002\" class=\"wp-image-3993\" style=\"width:746px;height:auto\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-84-e1750261545761.jpg 727w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/1-84-e1750261545761-300x92.jpg 300w\" sizes=\"auto, (max-width: 727px) 100vw, 727px\" \/><\/figure>\n\n\n\n<p class=\"has-small-font-size\"><strong>mTOR\u306b\u5bfe\u3059\u308b\u6297\u4f53<\/strong>\u3092\u4f7f\u7528\u3057\u305f\u3001<strong>(A) <\/strong>HEK293 (\u4e0d\u6b7b\u5316\u30d2\u30c8\u80da\u814e\u81d3) \u7d30\u80de\u62bd\u51fa\u7269\u306b\u304a\u3051\u308bmTOR\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d7\u30ed\u30c3\u30c8\u89e3\u6790\u3001<strong>(B)<\/strong> HeLa (\u4e0d\u6b7b\u5316\u30d2\u30c8\u5b50\u5bae\u9838\u304c\u3093) \u7d30\u80de\u306b\u304a\u3051\u308bmTOR\u306e\u514d\u75ab\u7d30\u80de\u5316\u5b66\u5206\u6790\u306b\u304a\u3044\u3066\u3001\u7d30\u80de\u3092\u3055\u3089\u306bAlexa Fluor\u2122 488\u6a19\u8b58\u30e4\u30ae\u6297\u30a6\u30b5\u30ae\u4e8c\u6b21\u6297\u4f53\u3067\u67d3\u8272\u3057\u3066\u691c\u51fa(a)\uff1a\u6838\u3092DAPI\u3067\u67d3\u8272(b)\uff1a\u30a2\u30af\u30c1\u30f3\u3092Alexa Fluor\u2122 594\u30d5\u30a1\u30ed\u30a4\u30b8\u30f3\u3067\u67d3\u8272(c)\uff1a\u7d30\u80de\u8cea\u304a\u3088\u3073\u6838\u5c40\u5728\u3092\u793a\u3059\u91cd\u306d\u5408\u308f\u305b\u753b\u50cf(d)\u3001<strong>(C) <\/strong>\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u89e3\u6790\u306b\u304a\u3051\u308b\u6297mTOR\u6297\u4f53\u6a19\u8b58HeLa\u7d30\u80de (\u5857\u308a\u3064\u3076\u3057) \u3068\u975e\u67d3\u8272\u7d30\u80de (\u9752) \u304a\u3088\u3073\u30a2\u30a4\u30bd\u30bf\u30a4\u30d7\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb (\u9ed2) \u3068\u306e\u6bd4\u8f03\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#e7e7e7;color:#e7e7e7\" \/>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9-\u304c\u3093\u5897\u6b96\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306e\u63a2\u7d22\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\">\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u304c\u3093\u5897\u6b96\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306e\u63a2\u7d22\u30ac\u30a4\u30c9\u30d6\u30c3\u30af<\/h2>\n\n\n\n<p>\u5f53\u8a18\u4e8b\u306f\u3001\u304c\u3093\u5897\u6b96\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u306e\u63a2\u7d22\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002\u4e0b\u8a18\u306e\u5185\u5bb9\u3092\u542b\u3080PDF\u306f\u4e0b\u8a18\u304b\u3089\u7121\u6599\u3067\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u9802\u3051\u307e\u3059\u3002<\/p>\n\n\n\n<p>\uff5e\u4e3b\u306a\u5185\u5bb9\uff5e<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u304c\u3093\u6982\u8ad6<\/li>\n\n\n\n<li>\u304c\u3093\u5897\u6b96\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054<\/li>\n\n\n\n<li>PI3K\/Akt\/mTOR\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def<\/li>\n\n\n\n<li>Ras\/Raf\/MEK\/ERK(MAPK)\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def<\/li>\n\n\n\n<li>Wnt\/\u03b2-\u30ab\u30c6\u30cb\u30f3 \u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def<\/li>\n\n\n\n<li>\u6297\u4f53\uff1a\u304c\u3093\u7814\u7a76\u306e\u305f\u3081\u306e\u5354\u529b\u306a\u30c4\u30fc\u30eb<\/li>\n<\/ol>\n\n\n\n<p><strong><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/antibody-cancer-guidebook-form.html?CID=bid_pca_aup_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7121\u6599\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9-\u304c\u3093\u7814\u7a76\u3092\u4fc3\u9032\u3055\u305b\u308b\u907a\u4f1d\u5b50\u89e3\u6790\u6280\u8853\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\">\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u304c\u3093\u7814\u7a76\u3092\u4fc3\u9032\u3055\u305b\u308b\u907a\u4f1d\u5b50\u89e3\u6790\u6280\u8853\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n\n\n\n<p>\u307e\u305f\u3001\u8907\u6570\u306e\u304c\u3093\u7814\u7a76\u9818\u57df\u306b\u304a\u3051\u308b\u8208\u5473\u6df1\u3044\u6700\u65b0\u306e\u767a\u898b\u3092\u53ef\u80fd\u306b\u3059\u308b\u907a\u4f1d\u5b50\u89e3\u6790\u6280\u8853\u3092\u7d39\u4ecb\u3059\u308b\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3082\u914d\u5e03\u3057\u3066\u3044\u307e\u3059\u3002\u4f75\u305b\u3066\u3054\u3089\u3093\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/cancer-genetic-tech-ebook-download.html?CID=bid_pca_aup_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7121\u6599\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n\n\n\n<p><span style=\"font-size: 8pt\">\u53c2\u8003\u6587\u732e\uff1a<br>1. Frumand DA, Rommel C (2014) PI3K and Cancer: lessons, challenges and opportunities. Nat Rev Drug&nbsp;Discov 13(2):140\u2013156.<br>2. LoPiccolo J, Blumenthal GM, Bernstein WB, Dennis PA (2008) Targeting the PI3K\/Akt\/mTOR Pathway:&nbsp;effective combinations and clinical considerations. Drug Resist Updat 11(1\u20132):32\u201350.<br>3. Polivka J Jr, Janku F (2014) Molecular targets for cancer therapy in the PI3K\/AKT\/mTOR Pathway.&nbsp;Pharmacol Ther 142(2):164\u2013175.<br>4. National Library of Medicine (US), Genetics Home Reference website (2012) Cowden syndrome. http:\/\/ghr.nlm.nih.gov\/condition\/cowden-syndrome<br>5. Stryer, L (1995) Biochemistry 4th ed, New York (NY): WH Freeman and Company. pp 265 and 344.<br>6. Rommel C, Camps M, Ji H (2007) PI3K delta and PI3K gamma: partners in crime in inflammation in&nbsp;rheumatoid arthritis and beyond? Nat Rev Immunol 7(3):191\u2013201.<br>7. Grabon A, Khan D, Bankaitis VA (2015) Phosphatidylinositol transfer proteins and instructive regulation of lipid&nbsp;kinase biology. Biochim Biophys Acta 1851(6):724-735.<br>8. Koyasu S (2003) The role of PI3K in immune cells. Nat Immunol 4(4):313\u2013319.<br>9. Vanhaesebroeck B, Stephens L, Hawkins P (2012) PI3K signaling: the path to discovery and understanding.&nbsp;Nat Rev Mol Cell Biol 13(3):195\u2013203.<br>10. Dorsam RT, Gutkind SJ (2007) G-protein-coupled receptors and cancer. Nat Rev Cancer 7(2):79\u201394.<br>11. Jabbour E, Ottmann OG, Deininger M Hochhaus A (2014) Targeting the phosphoinositide 3-kinase pathway&nbsp;in hematologic malignancies. Haematologica 99(1):7\u201318.<br>12. Karakas B, Bachman KE, Park BH (2006) Mutation of the PIK3CA oncogene in human cancers. Br J Cancer 94(4):455\u2013459.<br>13. Song MS, Salmena L, Pandolfi PP (2012) The functions and regulation of the PTEN tumour suppressor. Nat&nbsp;Rev Mol Cell Biol 13(5):283\u2013296.<br>14. Gu J, Tamura M, Pankov R et al. (1999) Shc and FAK differentially regulate cell motility and directionality&nbsp;modulated by PTEN. J Cell Biol 146(2):389\u2013403.<br>15. National Library of Medicine (US), Genetics Home Reference website (2012) Bannayan-Riley-Ruvalcaba&nbsp;syndrome. http:\/\/ghr.nlm.nih.gov\/condition\/bannayan-riley-ruvalcaba-syndrome<br>16. Rozakis-Adcock M, McGlade J, Mbamalu G et al. (1992) Association of the Shc and Grb2\/Sem5&nbsp;SH2-containing proteins is implicated in the activation of the Ras pathway by tyrosine kinases. Nature&nbsp;360(6405):689\u2013692.<br>17. National Library of Medicine (US), National Center for Biotechnology Information website, gene database&nbsp;(2015) SHC1 SHC (Src homology 2 domain containing) transforming protein 1 [Homo sapiens (human)].&nbsp;http:\/\/www.ncbi.nlm.nih.gov\/gene\/6464<br>18. Davies MA (2011) Regulation, role, and targeting of Akt in cancer. J Clin Oncol 29(35):4715\u20134717.<br>19. 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