{"id":9806,"date":"2021-08-13T17:07:29","date_gmt":"2021-08-13T08:07:29","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=9806"},"modified":"2021-08-26T17:03:20","modified_gmt":"2021-08-26T08:03:20","slug":"wtpicoreagentkit_protocol_ma_gsd_mkt_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/","title":{"rendered":"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb"},"content":{"rendered":"<p>\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u4f7f\u7528\u3057\u305f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306f\u73fe\u5728\u3082\u3063\u3068\u3082\u30dd\u30d4\u30e5\u30e9\u30fc\u306a\u65b9\u6cd5\u3067\u3001\u767a\u73fe\u6bd4\u8f03\u7814\u7a76\u3084\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u63a2\u7d22\u306b\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u5b9f\u7e3e\u304c\u3042\u308a\u307e\u3059\u3002\u8fd1\u5e74\u3082\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u3084IncRNA\u89e3\u6790\u3092\u4e2d\u5fc3\u306b\u30a2\u30ec\u30a4\u306f\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304a\u308a\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306e\u4e2d\u5fc3\u30d7\u30ec\u30fc\u30e4\u30fc\u3067\u3042\u308b\u3068\u3044\u3048\u307e\u3059\u3002<br \/>\n\u4eca\u56de\u306e\u30d6\u30ed\u30b0\u3067\u306f\u3001GeneChip\u2122 WT Pico Kit\u306e\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306b\u95a2\u3059\u308b\u624b\u5f15\u304d\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002GeneChip\u2122 WT Pico Kit\u306f\u30d5\u30ec\u30ad\u30b7\u30d6\u30eb\u3067\u9ad8\u611f\u5ea6\u306a\u30bf\u30fc\u30b2\u30c3\u30c8\u8abf\u88fd\u6cd5\u3067\u3001\u308f\u305a\u304b10\u7d30\u80de\u306b\u3088\u308b\u5168\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30c8\u30fc\u30e0\uff08WT\uff09\u30a2\u30ec\u30a4\u3092\u7528\u3044\u305f\u6b63\u78ba\u304b\u3064\u7cbe\u5bc6\u306a\u89e3\u6790\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002\u3053\u306e\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u53c2\u8003\u306b\u3001\u82e5\u3044\u7814\u7a76\u8005\u3055\u307e\u65b9\u306b\u3082\u6539\u3081\u3066\u3001GeneChip WT\u30a2\u30ec\u30a4\u306e\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306b\u95a2\u3059\u308b\u3042\u308c\u3053\u308c\u3092\u5e83\u304f\u77e5\u3063\u3066\u3044\u305f\u3060\u3051\u308c\u3070\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<p>\u5bfe\u8c61\u88fd\u54c1\uff1aApplied Biosystems\u2122 GeneChip\u2122 WT Pico Reagent Kit<\/p>\n<p>\u5bfe\u8c61\u88fd\u54c1\u756a\u53f7\uff1a902622\uff0812\u53cd\u5fdc\u5206\uff09\u3001902623\uff0830\u53cd\u5fdc\u5206\uff09<\/p>\n<p><a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/MAN0017893_703262_GeneChipWT_Pico_UG.pdf?cid=gsd_mic_get_r04_jp_cp1424_pjt7219_gsd00000_0so_blg_op_awa_og_s00_ma14_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u516c\u5f0f\u30e6\u30fc\u30b6\u30fc\u30ac\u30a4\u30c9<\/a>\u3068\u4f75\u305b\u3066\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u203bWT Pico eagent Kit\u306b\u306f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306f\u4ed8\u5c5e\u3057\u307e\u305b\u3093\u3002WT Pico Reagent Kit\u3068\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u30bb\u30c3\u30c8\u54c1\u3001\u3082\u3057\u304f\u306fWT Pico Reagent Kit\u306b\u5bfe\u5fdc\u3059\u308b\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u3054\u8cfc\u5165\u304f\u3060\u3055\u3044\u3002\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3068\u8a66\u85ac\u306e\u9078\u629e\u306b\u3064\u3044\u3066\u306f<a href=\"https:\/\/www.thermofisher.com\/document-connect\/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fbrochures%2FEMI06269-2_-Flyer_Expression_Reagent_Guide_HYPERLINK_Final.pdf&amp;title=QnJvY2h1cmU6IEV4cHJlc3Npb24gTWljcm9hcnJheSBSZWFnZW50IEd1aWRl?cid=gsd_mic_get_r04_jp_cp1424_pjt7219_gsd00000_0so_blg_op_awa_og_s00_ma14_Social_LAB\" target=\"_blank\" rel=\"noopener\">Expression Microarray Reagent Guide<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>\u3053\u306e\u30ad\u30c3\u30c8\u306e\u307b\u304b\u306b\u3001\u3042\u3089\u304b\u3058\u3081\u3054\u7528\u610f\u3044\u305f\u3060\u304f\u8a66\u85ac<\/h2>\n<p>\u30fb100%\u30a8\u30bf\u30ce\u30fc\u30eb<br \/>\n\u30fbApplide Biosystems\u2122 GeneChip\u2122 Hybridizatoin, Wash, and Stain kit \uff08\u88fd\u54c1\u756a\u53f7\u3000900720\uff0830\u53cd\u5fdc\u5206\uff09\uff09<\/p>\n<h2>\u30a2\u30c3\u30bb\u30a4\u306b\u4f7f\u7528\u3059\u308bRNA\u91cf<\/h2>\n<p><strong>IMPORTANT<\/strong><strong>\uff1a<br \/>\n<\/strong>Total RNA\u5bb9\u91cf\u306f5 \u03bcL\u4ee5\u4e0b\u306b\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\uff08poly-A RNA controls\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f3 \u03bcL\u4ee5\u4e0b\uff09\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9807\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t1-1024x267.png\" alt=\"\" width=\"760\" height=\"198\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t1-1024x267.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t1-300x78.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t1-768x200.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t1.png 1435w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9808\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t2-1024x770.png\" alt=\"\" width=\"760\" height=\"571\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t2-1024x770.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t2-300x226.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t2-768x578.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t2-1536x1155.png 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t2.png 1538w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9810\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t3-1024x359.png\" alt=\"\" width=\"760\" height=\"266\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t3-1024x359.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t3-300x105.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t3-768x269.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t3.png 1441w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<p><strong>TIP\uff1a<\/strong>PCR\u306e\u30b5\u30a4\u30af\u30eb\u6570\u306b\u95a2\u3057\u3066<strong><br \/>\n<\/strong>Total RNA\u30b5\u30f3\u30d7\u30eb\u306e\u54c1\u8cea\u304c\u4f4e\u304f\u3001\u3053\u306e\u30ad\u30c3\u30c8\u3092\u7528\u3044\u3066cRNA\u306e\u53ce\u91cf\u304c\u5341\u5206\u306b\u5f97\u3089\u308c\u306a\u304b\u3063\u305f\u5834\u5408\u3001Table C\u306ePCR\u30b5\u30a4\u30af\u30eb\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u306e\u30b5\u30a4\u30af\u30eb\u6570\u30921\uff5e2\u56de\u5897\u3084\u3059\u3068cRNA\u306e\u53ce\u91cf\u304c\u6539\u5584\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p><strong>TIP\uff1a<\/strong>\u8a66\u85ac\u8abf\u88fd\u306b\u95a2\u3057\u3066<strong><br \/>\n<\/strong>\u30fb\u30ad\u30c3\u30c8\u306e\u51cd\u7d50\u878d\u89e3\u306f3\u56de\u4ee5\u4e0b\u3067\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30fb\u9175\u7d20\u985e\u306f\u7a4f\u3084\u304b\u306b\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306b\u304b\u3051\u3066\u6df7\u5408\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30d0\u30c3\u30d5\u30a1\u30fc\u985e\u306f\u6c88\u6bbf\u7269\u304c\u306a\u304f\u306a\u308b\u307e\u3067\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306b\u304b\u3051\u3066\u3088\u304f\u6df7\u5408\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n<strong>\u30fb<\/strong>\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3084\u30b5\u30f3\u30d7\u30eb\u306f\u7a4f\u3084\u304b\u306b\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306b\u304b\u3051\u3066\u3001\u3088\u304f\u6df7\u5408\u3057\u305f\u5f8c\u306b\u7d20\u65e9\u304f\u30b9\u30d4\u30f3\u30c0\u30a6\u30f3\u3057\u3066\u3001\u6eb6\u6db2\u3092\u96c6\u3081\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30fb\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u3088\u308b\u30ed\u30b9\u306b\u5099\u3048\u308b\u305f\u3081\u306b10%\u7a0b\u5ea6\u904e\u5270\u306b\u8abf\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30fb\u53cd\u5fdc\u5f8c\u306f\u30b5\u30f3\u30d7\u30eb\u30c1\u30e5\u30fc\u30d6\u3092\u7c21\u6f54\u306b\u30b9\u30d4\u30f3\u30c0\u30a6\u30f3\u3057\u3001\u6b21\u306e\u30b9\u30c6\u30c3\u30d7\u306b\u9032\u3080\u524d\u306b\u306f\u6c37\u4e0a\u306b\u7f6e\u3044\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30fb\u9175\u7d20\u3092\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306b\u52a0\u3048\u308b\u306e\u306f\u53cd\u5fdc\u3092\u884c\u3046\u76f4\u524d\u306b\u884c\u3046\u3088\u3046\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>Section 1\uff1aPoly-A RNA Controls\u3001Total RNA\u306e\u6e96\u5099<\/h2>\n<p>1. \u4f7f\u7528\u3059\u308bTotal RNA\u91cf\u306b\u5fdc\u3058\u3066\u3001Poly-A RNA Controls\u3092\u5e0c\u91c8\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9811\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t4-1024x460.png\" alt=\"\" width=\"760\" height=\"341\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t4-1024x460.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t4-300x135.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t4-768x345.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t4.png 1439w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<p><strong>TIP\uff1a<\/strong>\u5e0c\u91c8\u3059\u308b\u6642\u306f\u6b63\u78ba\u6027\u3084\u4e00\u8cab\u6027\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306b2 uL\u672a\u6e80\u306e\u5bb9\u91cf\u3067\u306e\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u306f\u907f\u3051\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9812\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t5-1024x289.png\" alt=\"\" width=\"760\" height=\"214\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t5-1024x289.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t5-300x85.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t5-768x216.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t5.png 1437w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<h2>Section 2\uff1aFirst-Strand cDNA\u5408\u6210<\/h2>\n<p>1. \u6c37\u4e0a\u3067\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9813\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t6-1024x226.png\" alt=\"\" width=\"760\" height=\"168\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t6-1024x226.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t6-300x66.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t6-768x170.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t6.png 1372w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><br \/>\n2. \u6c37\u4e0a\u3067\u53cd\u5fdc\u306b\u4f7f\u7528\u3059\u308b\u30c1\u30e5\u30fc\u30d6\u3078\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b95 uL\u3092\u5206\u6ce8\u3057\u3001Table 2\u3067\u4f5c\u6210\u3057\u305fTotal RNA\/Poly-A Control Mixture\u3092\u52a0\u3048\u307e\u3059\u3002<br \/>\n3. First-Strand cDNA Synthesis program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306625\u2103\u30675\u5206\u300142\u2103\u306760\u5206\u30014\u2103\u30672\u5206\u4ee5\u4e0a\u306e\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002<br \/>\n4. \u53cd\u5fdc\u7d42\u4e86\u5f8c\u306f\u901f\u3084\u304b\u306bCleanup\u306e\u884c\u7a0b\u3078\u9032\u307f\u307e\u3059\u3002<br \/>\n5. \u6c37\u4e0a\u3067\u5404cDNA\u30b5\u30f3\u30d7\u30eb10 uL\u306bWT Pico Clean up Reagent 2 uL\u3092\u52a0\u3048\u307e\u3059\u3002\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u30672\u56de\u6ce8\u610f\u6df1\u304f\u6df7\u305c\u305f\u5f8c\u3001\u6ce1\u304c\u7acb\u305f\u306a\u3044\u3088\u3046\u306b\u6df7\u305c\u3001\u30b9\u30d4\u30f3\u30c0\u30a6\u30f3\u3092\u884c\u3044\u307e\u3059\u3002<br \/>\n6. Cleanup program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306637\u2103\u306730\u5206\u300180\u2103\u306710\u5206\u30014\u2103\u30672\u5206\u4ee5\u4e0a\u306e\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n<h2>Section 3\uff1a3&#8242; Adaptor cDNA\u5408\u6210<\/h2>\n<p>1. \u6c37\u4e0a\u3067\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002<br \/>\n<strong>NOTE\uff1a<\/strong>\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u3066\u3044\u308b\u9593\u306b\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u30d6\u30ed\u30c3\u30af\u6e29\u5ea6\u309215\u2103\u306b\u3042\u3089\u304b\u3058\u3081\u51b7\u5374\u3057\u3066\u304a\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u3075\u305f\u306b\u3064\u3044\u3066\u306f\u3001\u52a0\u71b1\u3092\u6b62\u3081\u308b\u304b\u3001\u3075\u305f\u306e\u6e29\u5ea6\u5236\u5fa1\u304c\u3067\u304d\u306a\u3044\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u5834\u5408\u306f\u3075\u305f\u3092\u958b\u3051\u305f\u307e\u307e\u3067\u53cd\u5fdc\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9815\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t7-1024x223.png\" alt=\"\" width=\"760\" height=\"166\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t7-1024x223.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t7-300x65.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t7-768x167.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t7.png 1368w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><br \/>\n2. \u6c37\u4e0a\u3067\u53cd\u5fdc\u306b\u4f7f\u7528\u3059\u308bFirst Strand cDNA\u30b5\u30f3\u30d7\u30eb12 \u03bcL\u306b\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b98 \u03bcL\u3092\u52a0\u3048\u307e\u3059\uff08Total volume 20 \u03bcL\uff09\u3002<br \/>\n3. 3\u2019 Adaptor cDNA Synthesis program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306615\u2103\u306715\u5206\u300135\u2103\u306715\u5206\u300170\u2103\u306710\u5206\u30014\u2103\u30672\u5206\u4ee5\u4e0a\u306e\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n<h2>Section 4\uff1aDouble-Strand cDNA\u5408\u6210<\/h2>\n<p>1. \u6c37\u4e0a\u3067\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9816\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t8-1024x224.png\" alt=\"\" width=\"760\" height=\"166\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t8-1024x224.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t8-300x66.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t8-768x168.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t8.png 1369w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><br \/>\n2. \u6c37\u4e0a\u3067\u54043\u2019 Adaptor cDNA\u30b5\u30f3\u30d7\u30eb20 \u03bcL\u306b\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b930 \u03bcL\u3092\u52a0\u3048\u307e\u3059\uff08Total volume 50 \u03bcL\uff09\u3002<br \/>\n3. Pre-IVT Amplification program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306695\u2103\u30672\u5206\u30016 or 9 or 12 cycles\u6570\u306b\u306694\u2103\u306730\u79d2\u306870\u2103\u30675\u5206\u306e\u53cd\u5fdc\u3092\u884c\u3044\u3001\u6700\u5f8c\u306f4\u2103\u30672\u5206\u4ee5\u4e0a\u7f6e\u304d\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u30bf\u30a4\u30d7\u3068Total RNA\u30a4\u30f3\u30d7\u30c3\u30c8\u91cf\u306b\u57fa\u3065\u304fPCR\u30b5\u30a4\u30af\u30eb\u306e\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u306b\u3064\u3044\u3066\u306f\u3001Table C\u3092\u53c2\u7167\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n4. \u53cd\u5fdc\u7d42\u4e86\u5f8c\u306f\u901f\u3084\u304b\u306bIn Vitro Transcription\u53cd\u5fdc\u306b\u9032\u307f\u307e\u3059\u3002<\/p>\n<h2>Section 5\uff1aIn Vitro Transcription\u306b\u3088\u308bcRNA\u5408\u6210<\/h2>\n<p>1. \u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u8abf\u88fd\u6642\u3001Double-Strand cDNA\u30b5\u30f3\u30d7\u30eb\u306f\u5ba4\u6e29\u306b5\u5206\u4ee5\u4e0a\u7f6e\u304d\u307e\u3059\u3002<\/p>\n<p><strong>NOTE<\/strong><strong>\uff1a<\/strong>IVT Buffer\u309210\u5206\u4ee5\u4e0a\u5ba4\u6e29\u306b\u7f6e\u3044\u3066\u304b\u3089\u3001\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9818\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t9-1024x222.png\" alt=\"\" width=\"760\" height=\"165\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t9-1024x222.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t9-300x65.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t9-768x166.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t9.png 1375w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<p>2. \u5ba4\u6e29\u3067\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002<br \/>\n3. \u5ba4\u6e29\u3067\u5404Double-Strand cDNA\u30b5\u30f3\u30d7\u30eb50 \u03bcL\u306b\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b930 \u03bcL\u3092\u52a0\u3048\u307e\u3059\uff08Total volume 80 \u03bcL\uff09\u3002<br \/>\n4. In Vitro Transcription cRNA program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306640\u2103\u306714\u6642\u9593\u306e\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002<br \/>\n5. \u53cd\u5fdc\u7d42\u4e86\u5f8c\u306fcRNA\u7cbe\u88fd\u306b\u9032\u3080\u304b\u3001\u901f\u3084\u304b\u306b\u51b7\u51cd\u4fdd\u5b58\u3057\u307e\u3059\u3002<\/p>\n<p><strong>TIP<\/strong><strong>\uff1a<\/strong>In Vitro Transcription\u306e14\u6642\u9593\u306e\u53cd\u5fdc\u306f\u300116\u6642\u9593\u307e\u3067\u5ef6\u9577\u304c\u53ef\u80fd\u3067\u3059\u3002<br \/>\n<strong>TIP<\/strong><strong>\uff1a<\/strong>STOPPING POINT\uff5ecRNA\u30b5\u30f3\u30d7\u30eb\u306f-20\u2103\u3067\u4e00\u6669\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002<\/p>\n<h2>Section 6\uff1acRNA\u7cbe\u88fd<\/h2>\n<p><strong>cRNA<\/strong><strong>\u7cbe\u88fd\u3092\u59cb\u3081\u308b\u524d\u306b\uff1a<br \/>\n<\/strong>\u30fb\u5c11\u306a\u304f\u3068\u308210\u5206\u9593\u306fNuclease-free Water\u309265\u2103\u306b\u3042\u3089\u304b\u3058\u3081\u52a0\u6e29\u3057\u3066\u304a\u304d\u307e\u3059\u3002<br \/>\n\u30fb\u7cbe\u88fd\u30d3\u30fc\u30ba\u306f\u4f7f\u7528\u524d\u306b\u3088\u304f\u6df7\u5408\u3057\u3066\u304a\u304d\u307e\u3059\u3002\u9069\u5f53\u91cf\u3092\u5206\u5272\u3057\u3066\u5ba4\u6e29\u3067\u4fdd\u6301\u3057\u307e\u3059\u3002\u5404\u53cd\u5fdc\u30b5\u30f3\u30d7\u30eb\u306b\u5bfe\u3057\u3066<br \/>\n140 \u03bcL+10%\u904e\u5270\u91cf\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<br \/>\n\u30fb80%\u30a8\u30bf\u30ce\u30fc\u30eb\u6d17\u6d44\u6db2\u306f\u5fc5\u305a\u7528\u6642\u8abf\u88fd\u3057\u307e\u3059\u3002\u5404\u53cd\u5fdc\u30b5\u30f3\u30d7\u30eb\u5bfe\u3057\u3066600 \u03bcL+10%\u904e\u5270\u91cf\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p><strong>NOTE<\/strong><strong>\uff1a<\/strong>\u3059\u3079\u3066\u306e\u884c\u7a0b\u3092\u5ba4\u6e29\u3067\u884c\u3044\u307e\u3059\u3002<\/p>\n<p>\u7cbe\u88fd\u30d3\u30fc\u30ba\u3078\u306ecRNA\u7d50\u5408<\/p>\n<ol>\n<li>\u7cbe\u88fd\u30d3\u30fc\u30ba\u3092\u518d\u61f8\u6fc1\u3059\u308b\u305f\u3081\u306b\u3088\u304f\u6df7\u5408\u3057\u307e\u3059\u3002\u7cbe\u88fd\u30d3\u30fc\u30ba140 \u03bcL\u3092\u4e38\u5e95\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u306e\u672a\u4f7f\u7528\u30a6\u30a7\u30eb\u306b\u79fb\u3057\u307e\u3059\u3002\u7d9a\u3044\u3066\u30d3\u30fc\u30ba\u306e\u5165\u3063\u305f\u30a6\u30a7\u30eb\u306b\u5404cRNA\u30b5\u30f3\u30d7\u30eb80 \u03bcL\u3092\u52a0\u3048\u3066\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u3067\u6df7\u5408\u3057\u307e\u3059\u3002<\/li>\n<li>\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u309210\u56de\u884c\u3063\u3066\u3088\u304f\u6df7\u5408\u3057\u305f\u5f8c\u300110\u5206\u9593\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u96c6\u3081\u308b\u305f\u3081\u306b\u30d7\u30ec\u30fc\u30c8\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u306b\u79fb\u52d5\u3055\u305b\u3066\u30015\u5206\u9593\u7a0b\u5ea6\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u5206\u6563\u3055\u305b\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u3066\u4e0a\u6e05\u3092\u56de\u53ce\u3057\u3066\u5ec3\u68c4\u3057\u307e\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u306f\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u306b\u9759\u7f6e\u3057\u3066\u304a\u304d\u307e\u3059\u3002<\/li>\n<\/ol>\n<p>\u7cbe\u88fd\u30d3\u30fc\u30ba\u306e\u6d17\u6d44<\/p>\n<ol>\n<li>\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u3067\u5404\u30a6\u30a7\u30eb\u306b80%\u30a8\u30bf\u30ce\u30fc\u30eb\u6d17\u6d44\u6db2200 \u03bcL\u3092\u52a0\u3048\u306630\u79d2\u9593\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u5206\u6563\u3055\u305b\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u3066\u3086\u3063\u304f\u308a\u306880%\u30a8\u30bf\u30ce\u30fc\u30eb\u6d17\u6d44\u6db2\u3092\u56de\u53ce\u3057\u3066\u5ec3\u68c4\u3057\u307e\u3059\u3002<\/li>\n<li>\u30b9\u30c6\u30c3\u30d7A\u3068\u30b9\u30c6\u30c3\u30d7B\u3092\u7e70\u308a\u8fd4\u3057\u3001\u8a083\u56de\u306e\u6d17\u6d44\u3092\u884c\u3044\u307e\u3059\u3002\u6700\u7d42\u6d17\u6d44\u6db2\u306f\u5b8c\u5168\u306b\u9664\u53bb\u3057\u307e\u3059\u3002<\/li>\n<li>\u6db2\u4f53\u304c\u898b\u3048\u306a\u304f\u306a\u308b\u307e\u30675\u5206\u9593\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u3067\u98a8\u4e7e\u3055\u305b\u307e\u3059\u3002\u72b6\u6cc1\u306b\u3088\u3063\u3066\u306f\u3055\u3089\u306b\u6642\u9593\u3092\u8981\u3059\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u30d3\u30fc\u30ba\u3092\u4e7e\u304b\u3057\u3059\u304e\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u307e\u3059\u3002<\/li>\n<\/ol>\n<p>cRNA\u306e\u6eb6\u96e2<\/p>\n<ol>\n<li>\u30d7\u30ec\u30fc\u30c8\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u304b\u3089\u4e0b\u308d\u3057\u307e\u3059\u3002\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u3001\u3042\u3089\u304b\u3058\u308165\u2103\u306b\u52a0\u6e29\u3057\u3066\u304a\u3044\u305fNuclease-free Water 27 \u03bcL\u3092\u52a0\u3048\u30661\u5206\u9593\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u309210\u56de\u884c\u3063\u3066\u3001\u3088\u304f\u6df7\u5408\u3057\u307e\u3059\u3002<\/li>\n<li>\u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u96c6\u3081\u308b\u305f\u3081\u306b\u30d7\u30ec\u30fc\u30c8\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u306b\u79fb\u52d5\u3055\u305b\u3066\u30015\u5206\u9593\u7a0b\u5ea6\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u6eb6\u96e2\u3055\u308c\u305fcRNA\u304c\u542b\u3093\u3060\u4e0a\u6e05\u3092nuclease-free\u306e\u30c1\u30e5\u30fc\u30d6\u306b\u79fb\u3057\u307e\u3059\u3002<\/li>\n<li>\u7cbe\u88fd\u3055\u308c\u305fcRNA\u3092\u6c37\u4e0a\u306b\u7f6e\u304d\u3001\u5b9a\u91cf\u306b\u9032\u307f\u307e\u3059\u3002<\/li>\n<\/ol>\n<p><strong>TIP<\/strong><strong>\uff1a<\/strong>STOPPING POINT\uff5e\u7cbe\u88fd\u3055\u308c\u305fcRNA\u30b5\u30f3\u30d7\u30eb\u306f-20\u2103\u3067\u4e00\u6669\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002\u9577\u671f\u4fdd\u5b58\u3059\u308b\u5834\u5408\u306f\u3001-80\u2103\u3067\u4fdd\u5b58\u3057\u307e\u3059\u3002<\/p>\n<h2>Section 7\uff1acRNA\u53ce\u91cf\u306e\u78ba\u8a8d<\/h2>\n<p>Thermo Scientific\u2122 NanoDrop\u2122\u00a0Spectrophotometer\u306a\u3069\u306e\u6e2c\u5b9a\u6a5f\u5668\u3067260 nm\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u3092\u884c\u3063\u3066\u3001cRNA\u30b5\u30f3\u30d7\u30eb\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u6b21\u306e\u5de5\u7a0b\u306b\u9032\u3080\u305f\u3081\u306b\u306f\u300120 ug\u4ee5\u4e0a\uff08833 ng\/\u03bcL\u4ee5\u4e0a\uff09\u306ecRNA\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<h2>Section 8\uff1a2nd-Cycle ss-cDNA\u5408\u6210<\/h2>\n<p>1. \u6c37\u4e0a\u3067Nuclease-free Water\u3092\u4f7f\u3063\u3066cRNA 20 ug\u309224 \u03bcL\u5bb9\u91cf\u306b\u8abf\u6574\u3057\u307e\u3059\u3002<br \/>\n2. \u6c37\u4e0a\u3067\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9819\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t10-1024x270.png\" alt=\"\" width=\"760\" height=\"200\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t10-1024x270.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t10-300x79.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t10-768x203.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t10.png 1375w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><br \/>\n3. \u6c37\u4e0a\u3067\u5404cRNA\u30b5\u30f3\u30d7\u30eb24 \u03bcL\u306b\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b916 \u03bcL\u3092\u52a0\u3048\u307e\u3059\uff08Total volume 40 \u03bcL\uff09\u3002<br \/>\n4. 2nd-Cycle ss-cDNA Synthesis program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306625\u2103\u306710\u5206\u300142\u2103\u306790\u5206\u300170\u2103\u306710\u5206\u306e\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002<br \/>\n5. \u901f\u3084\u304b\u306bRNA Hydrolysis\u306b\u9032\u307f\u307e\u3059\u3002<\/p>\n<h2>Section 9\uff1aRNase H\u3092\u7528\u3044\u305fRNA Hydrolysis<\/h2>\n<p>1. \u6c37\u4e0a\u3067\u54042nd-Cycle ss-cDNA\u30b5\u30f3\u30d7\u30eb40 uL\u306bRNase H 4 \u03bcL\u3092\u52a0\u3048\u307e\u3059\u3002<br \/>\n2. \u3088\u304f\u6df7\u5408\u3057\u305f\u5f8c\u3001\u30b9\u30d4\u30f3\u30c0\u30a6\u30f3\u3057\u307e\u3059\u3002<br \/>\n3. RNA Hydrolysis program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306637\u2103\u306745\u5206\u300195\u2103\u30675\u5206\u306e\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002<br \/>\n4. Hydrolyzed 2nd-Cycle ss-cDNA\u30b5\u30f3\u30d7\u30eb\u3092\u6c37\u4e0a\u306b\u7f6e\u304d\u307e\u3059\u3002<br \/>\n5. \u6c37\u4e0a\u3067\u5404Hydrolyzed 2nd-Cycle ss-cDNA\u30b5\u30f3\u30d7\u30eb44 \u03bcL\u306bNuclease-free Water 11 \u03bcL\u3092\u52a0\u3048\u307e\u3059\uff08Total volume 55 \u03bcL\uff09\u3002<br \/>\n6. \u3088\u304f\u6df7\u5408\u3057\u305f\u5f8c\u30012nd-Cycle ss-cDNA\u7cbe\u88fd\u306b\u9032\u3080\u304b\u3001\u901f\u3084\u304b\u306b\u51b7\u51cd\u4fdd\u5b58\u3057\u307e\u3059\u3002<\/p>\n<p><strong>TIP\uff1a<\/strong>STOPPING POINT\uff5eHydrolyzed 2nd-Cycles ss-cDNA\u30b5\u30f3\u30d7\u30eb\u306f-20\u2103\u3067\u4e00\u6669\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002<\/p>\n<h2>Section 10\uff1a2nd-Cycle ss-cDNA\u7cbe\u88fd<\/h2>\n<p><strong>ss-cDNA\u7cbe\u88fd\u3092\u59cb\u3081\u308b\u524d\u306b\uff1a<\/strong><strong><br \/>\n<\/strong>\u30fbNuclease-free Water\u3092\u3042\u3089\u304b\u3058\u308165\u2103\u3067\u5c11\u306a\u304f\u3068\u308210\u5206\u9593\u52a0\u6e29\u3057\u3066\u304a\u304d\u307e\u3059\u3002<br \/>\n\u30fb\u7cbe\u88fd\u30d3\u30fc\u30ba\u306f\u4f7f\u7528\u524d\u306b\u3088\u304f\u6df7\u5408\u3057\u3066\u304a\u304d\u307e\u3059\u3002\u9069\u5f53\u91cf\u3092\u5206\u5272\u3057\u3066\u5ba4\u6e29\u3067\u4fdd\u6301\u3057\u307e\u3059\u3002\u5404\u53cd\u5fdc\u30b5\u30f3\u30d7\u30eb\u306b\u5bfe\u3057\u3066100 \u03bcL+10%\u904e\u5270\u91cf\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<br \/>\n\u30fb80%\u30a8\u30bf\u30ce\u30fc\u30eb\u6d17\u6d44\u6db2\u306f\u5fc5\u305a\u7528\u6642\u8abf\u88fd\u3057\u307e\u3059\u3002\u5404\u53cd\u5fdc\u30b5\u30f3\u30d7\u30eb\u5bfe\u3057\u3066600 \u03bcL+10%\u904e\u5270\u91cf\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p><strong>NOTE<\/strong><strong>\uff1a<\/strong>\u3059\u3079\u3066\u306e\u884c\u7a0b\u3092\u5ba4\u6e29\u3067\u884c\u3044\u307e\u3059\u3002<\/p>\n<p>\u7cbe\u88fd\u30d3\u30fc\u30ba\u3078\u306ess-cDNA\u7d50\u5408<\/p>\n<ol>\n<li>\u7cbe\u88fd\u30d3\u30fc\u30ba\u3092\u518d\u61f8\u6fc1\u3059\u308b\u305f\u3081\u306b\u3088\u304f\u6df7\u5408\u3057\u305f\u5f8c\u3001100 mL\u3092\u4e38\u5e95\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u306e\u672a\u4f7f\u7528\u30a6\u30a7\u30eb\u306b\u79fb\u3057\u307e\u3059\u3002\u7d9a\u3044\u3066\u30d3\u30fc\u30ba\u306e\u5165\u3063\u305f\u30a6\u30a7\u30eb\u306b\u5404ss-cDNA\u30b5\u30f3\u30d7\u30eb55 \u03bcL\u3092\u52a0\u3048\u3066\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u3067\u6df7\u5408\u3057\u307e\u3059\u3002<\/li>\n<li>\u5404\u30b5\u30f3\u30d7\u30eb\u306b100%\u30a8\u30bf\u30ce\u30fc\u30eb150 mL\u3092\u52a0\u3048\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u309210\u56de\u884c\u3063\u3066\u3001\u3088\u304f\u6df7\u5408\u3057\u307e\u3059\u300220\u5206\u9593\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u96c6\u3081\u308b\u305f\u3081\u306b\u30d7\u30ec\u30fc\u30c8\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u306b\u79fb\u52d5\u3055\u305b\u3066\u30015\u5206\u9593\u7a0b\u5ea6\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u5206\u6563\u3055\u305b\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u3066\u4e0a\u6e05\u3092\u56de\u53ce\u3057\u3066\u5ec3\u68c4\u3057\u307e\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u306f\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u306b\u9759\u7f6e\u3057\u3066\u304a\u304d\u307e\u3059\u3002<\/li>\n<\/ol>\n<p>\u7cbe\u88fd\u30d3\u30fc\u30ba\u306e\u6d17\u6d44<\/p>\n<ol>\n<li>\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u3067\u5404\u30a6\u30a7\u30eb\u306b80%\u30a8\u30bf\u30ce\u30fc\u30eb\u6d17\u6d44\u6db2200 \u03bcL\u3092\u52a0\u3048\u306630\u79d2\u9593\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u5206\u6563\u3055\u305b\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u3066\u3086\u3063\u304f\u308a\u306880%\u30a8\u30bf\u30ce\u30fc\u30eb\u6d17\u6d44\u6db2\u3092\u56de\u53ce\u3057\u3066\u5ec3\u68c4\u3057\u307e\u3059\u3002<\/li>\n<li>\u30b9\u30c6\u30c3\u30d7A\u3068\u30b9\u30c6\u30c3\u30d7B\u3092\u7e70\u308a\u8fd4\u3057\u3001\u8a083\u56de\u306e\u6d17\u6d44\u3092\u884c\u3044\u307e\u3059\u3002\u6700\u7d42\u6d17\u6d44\u6db2\u306f\u5b8c\u5168\u306b\u9664\u53bb\u3057\u307e\u3059\u3002<\/li>\n<li>\u6db2\u4f53\u304c\u898b\u3048\u306a\u304f\u306a\u308b\u307e\u30675\u5206\u9593\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u3067\u98a8\u4e7e\u3055\u305b\u307e\u3059\u3002\u72b6\u6cc1\u306b\u3088\u3063\u3066\u306f\u3055\u3089\u306b\u6642\u9593\u3092\u8981\u3059\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u30d3\u30fc\u30ba\u3092\u4e7e\u304b\u3057\u3059\u304e\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u307e\u3059\u3002<\/li>\n<\/ol>\n<p>ss-cDNA\u306e\u6eb6\u96e2<\/p>\n<ol>\n<li>\u30d7\u30ec\u30fc\u30c8\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u304b\u3089\u4e0b\u308d\u3057\u307e\u3059\u3002\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u3001\u3042\u3089\u304b\u3058\u308165\u2103\u306b\u52a0\u6e29\u3057\u3066\u304a\u3044\u305fNuclease-free Water 30 \u03bcL\u3092\u52a0\u3048\u30661\u5206\u9593\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u309210\u56de\u884c\u3063\u3066\u3001\u3088\u304f\u6df7\u5408\u3057\u307e\u3059\u3002<\/li>\n<li>\u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u96c6\u3081\u308b\u305f\u3081\u306b\u30d7\u30ec\u30fc\u30c8\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u306b\u79fb\u52d5\u3055\u305b\u3066\u30015\u5206\u9593\u7a0b\u5ea6\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n<li>\u6eb6\u96e2\u3055\u308c\u305fss-cDNA\u304c\u542b\u3093\u3060\u4e0a\u6e05\u3092nuclease-free\u306e\u30c1\u30e5\u30fc\u30d6\u306b\u79fb\u3057\u307e\u3059\u3002<\/li>\n<li>\u7cbe\u88fd\u3055\u308c\u305fss-cDNA\u3092\u6c37\u4e0a\u306b\u7f6e\u304d\u3001\u5b9a\u91cf\u306b\u9032\u307f\u307e\u3059\u3002<\/li>\n<\/ol>\n<p><strong>TIP<\/strong><strong>\uff1a<\/strong>STOPPING POINT\uff5e\u7cbe\u88fd\u3055\u308c\u305fss-cDNA\u30b5\u30f3\u30d7\u30eb\u306f-20\u2103\u3067\u4e00\u6669\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002<\/p>\n<h2>Section 11\uff1ass-cDNA\u53ce\u91cf\u306e\u78ba\u8a8d<\/h2>\n<p>Thermo Scientific\u2122 NanoDrop\u2122\u00a0Spectrophotometer\u306a\u3069\u306e\u6e2c\u5b9a\u6a5f\u5668\u3067260 nm\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u3092\u884c\u3063\u3066\u3001ss-cDNA\u30b5\u30f3\u30d7\u30eb\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u6b21\u306e\u5de5\u7a0b\u306b\u9032\u3080\u305f\u3081\u306b\u306f\u30015.5 ug\u4ee5\u4e0a\u306ess-cDNA\u304c\u5fc5\u8981\u3067\u3059\u3002<br \/>\n\u203b\u30d3\u30fc\u30ba\u7cbe\u88fd\u5f8c\u306e\u6fc3\u5ea6\u6e2c\u5b9a\u306b\u3088\u308b\u6db2\u91cf\u30ed\u30b9\u306a\u3069\u3092\u8003\u616e\u3059\u308b\u3068\u30015.5 \u03bcg\u4ee5\u4e0a\u3092\u78ba\u4fdd\u3059\u308b\u305f\u3081\u306b\u3001\u5438\u5149\u5ea6\u6e2c\u5b9a\u6642\u306ess-cDNA\u6fc3\u5ea6\u306f\u76ee\u5b89\u3068\u3057\u3066212 ng\/\u03bcL\u4ee5\u4e0a\u5fc5\u8981\u3067\u3059\u3002\u304e\u308a\u304e\u308a\u306e\u5834\u5408\u306f\u5fc5\u8981\u91cf\u306b\u8db3\u308a\u308b\u304b\u8a08\u7b97\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>Section 12\uff1ass-cDNA\u306e\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u5316\u3068\u30e9\u30d9\u30eb\u5316<\/h2>\n<p>1. \u6c37\u4e0a\u3067Nuclease-free Water\u3092\u4f7f\u3063\u3066ss-cDNA 5.5 ug\u3092 46 \u03bcL\u5bb9\u91cf\u306b\u8abf\u6574\u3057\u307e\u3059\u3002<br \/>\n2. \u6c37\u4e0a\u3067\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9823\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t11-1024x222.png\" alt=\"\" width=\"760\" height=\"165\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t11-1024x222.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t11-300x65.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t11-768x167.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t11.png 1372w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><br \/>\n3. ss-cDNA\u30b5\u30f3\u30d7\u30eb5.5 \u03bcL\u306b\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b914 \u03bcL\u3092\u52a0\u3048\u307e\u3059\uff08Total volume 60 \u03bcL\uff09\u3002<br \/>\n4. Fragmentation and Labeling program\uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306637\u2103\u306760\u5206\u300193\u2103\u30672\u5206\u306e\u53cd\u5fdc\u3092\u884c\u3044\u3001\u53cd\u5fdc\u7d42\u4e86\u5f8c\u306f\u30b5\u30f3\u30d7\u30eb\u3092\u6c37\u4e0a\u306b\u7f6e\u304d\u307e\u3059\u3002<br \/>\n5. \u6c37\u4e0a\u306b\u30b5\u30f3\u30d7\u30eb\u3092\u7f6e\u3044\u3066Hybridization\u306b\u9032\u3080\u304b\u3001\u901f\u3084\u304b\u306b\u51b7\u51cd\u4fdd\u5b58\u3057\u307e\u3059\u3002<\/p>\n<p><strong>TIP<\/strong><strong>\uff1a<\/strong>STOPPING POINT\uff5eFragmented and labeled ss-cDNA\u30b5\u30f3\u30d7\u30eb\u306f-20\u2103\u3067\u4e00\u6669\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002\u9577\u671f\u4fdd\u5b58\u3059\u308b\u5834\u5408\u306f\u3001\u7cbe\u88fd\u3055\u308c\u305fss-cDNA\u3067\u4fdd\u5b58\u3059\u308b\u3053\u3068\u3092\u63a8\u5968\u3057\u307e\u3059\u3002<\/p>\n<h2>Section 13\uff1a\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3<\/h2>\n<p><strong>NOTE<\/strong><strong>\uff1a<br \/>\n<\/strong>\u30fb\u8a66\u85ac\u304c\u4f7f\u7528\u524d\u306b\u306f\u5b8c\u5168\u306b\u878d\u89e3\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002DMSO\u306f2\uff5e8\u2103\u3067\u4fdd\u5b58\u3059\u308b\u3068\u56fa\u307e\u308b\u306e\u3067\u3001\u4f7f\u7528\u5f8c\u306f\u5ba4\u6e29\u3067\u4fdd\u5b58\u3057\u305f\u65b9\u304c\u4f7f\u3044\u3084\u3059\u3044\u3067\u3059\u3002<br \/>\n\u30fb\u3053\u3053\u306e\u5de5\u7a0b\u3067\u306f\u5225\u9014\u8cfc\u5165\u3057\u3066\u3044\u305f\u3060\u3044\u305fGeneChip Hybridization, Wash and Stain Kit\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002<\/p>\n<p>1. 20\u00d7Hybridization Controls\u3092Hybridization Control program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306665\u2103\u30675\u5206\u52a0\u71b1\u3057\u307e\u3059\u3002<br \/>\n<strong>TIP<\/strong><strong>\uff1a<\/strong>\u30ad\u30c3\u30c8\u3054\u8cfc\u5165\u5f8c\u3001\u6700\u521d\u306b65\u2103\u30675\u5206\u52a0\u71b1\u3057\u305f\u5f8c\u306f1.5 mL\u30c1\u30e5\u30fc\u30d6\u306b150 \u03bcL\u305a\u3064\u5c0f\u5206\u3051\u306b\u3057\u3066\u4fdd\u5b58\u3057\u3066\u304a\u304f\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002\u51cd\u7d50\u878d\u89e3\u30923\u56de\u4ee5\u5185\u306b\u6291\u3048\u308b\u305f\u3081\u3067\u3059\u3002<br \/>\n2. \u5ba4\u6e29\u3067Hybridization Master Mix\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002\u4f7f\u7528\u3059\u308b\u30a2\u30ec\u30a4\u306b\u3088\u3063\u3066\u91cf\u304c\u7570\u306a\u308b\u305f\u3081\u3001Table 9\u306b\u5f93\u3063\u3066\u8abf\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9824\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t12-1024x425.png\" alt=\"\" width=\"760\" height=\"315\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t12-1024x425.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t12-300x125.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t12-768x319.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t12-1536x638.png 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t12.png 1543w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><br \/>\n3. \u5ba4\u6e29\u3067\u3001\u4f7f\u7528\u3059\u308bArray\u306e\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306b\u5bfe\u5fdc\u3057\u305f\u91cf\u306e\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u3055\u308c\u305fss-cDNA\u30b5\u30f3\u30d7\u30eb\u304c\u5165\u3063\u305f\u30c1\u30e5\u30fc\u30d6\u306bHybridization Master Mix\u3092\u6dfb\u52a0\u3057\u3066\u3001Hybridization Cocktail\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9825\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t13-1024x306.png\" alt=\"\" width=\"760\" height=\"227\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t13-1024x306.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t13-300x90.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t13-768x230.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t13.png 1381w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><br \/>\n<strong>TIP<\/strong><strong>\uff1a<\/strong>STOPPING POINT\uff5eHybridization Cocktail\u306f-20\u2103\u30671\u30ab\u6708\u7a0b\u5ea6\u3001-80\u2103\u30671\u5e74\u7a0b\u5ea6\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002<br \/>\n4. \u7a4f\u3084\u304b\u306b\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306b\u304b\u3051\u3066\u3001\u3088\u304f\u6df7\u5408\u3057\u307e\u3059\u3002<br \/>\n5. Hybridization Cocktail\u3092Hybridization Cocktail program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u3066\u300199\u2103\uff08\u30c1\u30e5\u30fc\u30d6\u306e\u5834\u5408\uff09\u307e\u305f\u306f95\u2103\uff08\u30d7\u30ec\u30fc\u30c8\u306e\u5834\u5408\uff09\u30675\u5206\u300145\u2103\u30675\u5206\u52a0\u71b1\u3057\u307e\u3059\u3002<br \/>\n6. \u52a0\u71b1\u5f8c\u3001\u77ed\u6642\u9593\u306e\u9060\u5fc3\u3092\u884c\u3044\u307e\u3059\u3002<br \/>\n7.\u5404\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306b\u5bfe\u5fdc\u3057\u305f\u91cf\u3092Array\u306b\u6ce8\u5165\u3057\u307e\u3059\u3002\u6ce8\u5165\u5f8c\u306f\u6ce8\u5165\u53e3\u306eSepta\u306b\u30bf\u30d5\u30b9\u30dd\u30c3\u30c8\u3092\u8cbc\u308a\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9826\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t14-1024x148.png\" alt=\"\" width=\"760\" height=\"110\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t14-1024x148.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t14-300x43.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t14-768x111.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t14.png 1312w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><strong>TIP<\/strong><strong>\uff1a<\/strong>169 or 400-Format\u306e\u5834\u5408\u3001\u82f1\u6587\u30d7\u30ed\u30c8\u30b3\u30eb\u3067\u306f\u6ce8\u5165\u91cf\u304c80 \u03bcL\u3068\u306a\u3063\u3066\u304a\u308a\u307e\u3059\u304c\u300190 \u03bcL\u306e\u6ce8\u5165\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u4e2d\u306e\u84b8\u767a\u306b\u3088\u308b\u6790\u51fa\u3092\u9632\u304e\u3084\u3059\u304f\u3059\u308b\u305f\u3081\u3067\u3059\u3002<br \/>\n8. Hybridization Oven\u306745\u2103\u300160 rpm\u306716\u6642\u9593\u306eHybridization\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n<h2>Section 14\uff1a\u6d17\u6d44\u3068\u67d3\u8272\u3001\u30b9\u30ad\u30e3\u30f3<\/h2>\n<p>\u30fb\u30b5\u30f3\u30d7\u30eb\u60c5\u5831\u767b\u9332\u65b9\u6cd5\u3084Applide Biosystems\u2122 GeneChip Fluidics Station\u3001Scanner\u306e\u64cd\u4f5c\u65b9\u6cd5\u306b\u3064\u3044\u3066\u306f\u3001GeneChip<span style=\"font-size: 10.8333px\">\u2122<\/span>\u00a0Command Console \uff08GCC\u307e\u305f\u306fAGCC\uff09 \u30af\u30a4\u30c3\u30af\u30b9\u30bf\u30fc\u30c8\u30ac\u30a4\u30c9\u3067\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30fb\u3053\u3053\u3067\u306f\u6d17\u6d44\u3068\u67d3\u8272\u4f5c\u696d\u3067\u4f7f\u7528\u3059\u308b\u8a66\u85ac\u985e\u306e\u5206\u6ce8\u4f5c\u696d\u306a\u3069\u3092\u8a18\u8f09\u3057\u307e\u3059\u3002<\/p>\n<p>1.\u00a0 Fluidics Station\u306e\u96fb\u6e90\u3092\u5165\u308c\u3001Wash A\u3001Wash B\u3001\u8d85\u7d14\u6c34\u306e\u5165\u3063\u305f\u30dc\u30c8\u30eb\u3092\u30bb\u30c3\u30c8\u3057\u3066\u3001GCC Fluidics Control<sup>\u203b<\/sup>\u3092\u8d77\u52d5\u3057\u3001Prime\u3092\u958b\u59cb\u3057\u307e\u3059\u3002<br \/>\n\u203bAGCC Fluidics Control\u3068\u3082\u547c\u3073\u307e\u3059\u3002<br \/>\n2. Hybridization\u3092\u884c\u3063\u3066\u3044\u308bArray\u306e\u679a\u6570\u306b\u5408\u308f\u305b\u3066\u3001Stain Cocktail 1\u3001Stain Cocktail 2\u3001Array Holding Buffer\u3092\u305d\u308c\u305e\u308c\u5206\u6ce8\u3057\u305fvial\u3092\u6e96\u5099\u3057\u307e\u3059\u3002<br \/>\n\u30fbStain Cocktail 1\u3000600 \u03bcL \uff08\u906e\u5149\u30c1\u30e5\u30fc\u30d6\uff09<br \/>\n\u30fbStain Cocktail 2\u3000600 \u03bcL \uff08\u900f\u660e\u30c1\u30e5\u30fc\u30d6\uff09<br \/>\n\u30fbArray Holding Buffer 800 \u03bcL \uff08\u900f\u660e\u30c1\u30e5\u30fc\u30d6\uff09<br \/>\n<strong>TIP<\/strong><strong>\uff1a<\/strong>Stain Cocktail 1\u7528\u306b\u906e\u5149\u30c1\u30e5\u30fc\u30d6\u306e\u3054\u7528\u610f\u304c\u96e3\u3057\u3044\u5834\u5408\u306f\u3001\u900f\u660e\u30c1\u30e5\u30fc\u30d6\u306b\u5165\u308c\u3001\u4f7f\u7528\u76f4\u524d\u307e\u3067\u906e\u5149\u306e\u74b0\u5883\u4e0b\u306b\u7f6e\u3044\u3066\u304a\u3044\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n3. Hybridization\u304c\u7d42\u4e86\u3057\u305fArray\u3092Hybridization Oven\u304b\u3089\u53d6\u308a\u51fa\u3057\u3066\u30bf\u30d5\u30b9\u30dd\u30c3\u30c8\u3092\u306f\u304c\u3057\u3001Hybridization Cocktail\u3092\u56de\u53ce\u3057\u307e\u3059\uff08\u30c7\u30fc\u30bf\u304c\u78ba\u5b9f\u306b\u53d6\u308c\u305f\u3053\u3068\u3092\u78ba\u8a8d\u3067\u304d\u308b\u307e\u3067\u306f-20\u2103\u3067\u4fdd\u5b58\uff09\u3002<br \/>\n4. Hybridization Cocktail\u3092\u56de\u53ce\u3057\u305f\u5f8c\u306f\u3001\u5404\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306b\u5bfe\u5fdc\u3057\u305f\u91cf\u306eWash A\u3092Array\u5185\u306b\u6ce8\u5165\u3057\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9827\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t15-1024x149.png\" alt=\"\" width=\"760\" height=\"111\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t15-1024x149.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t15-300x44.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t15-768x112.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t15.png 1307w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/>5. GCC Fluidics Control\u3067Wash and Stain\u3092\u958b\u59cb\u3057\u307e\u3059\u3002<br \/>\nArray\u306eFormat\u306b\u3088\u3063\u3066\u4f7f\u7528\u3059\u308b\u30d7\u30ed\u30c8\u30b3\u30eb\u304c\u7570\u306a\u308a\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-9828\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t16-1024x143.png\" alt=\"\" width=\"760\" height=\"106\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t16-1024x143.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t16-300x42.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t16-768x107.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/t16.png 1376w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><br \/>\n\u307e\u305f\u3001\u3053\u306e\u30bf\u30a4\u30df\u30f3\u30b0\u3067Scanner\u306e\u96fb\u6e90\u3092\u5165\u308c\u3066\u304a\u304d\u307e\u3059\u3002<br \/>\n6. Fluidics Station\u306e\u6db2\u6676\u753b\u9762\u306bEJECT &amp; INSPECT CARTRIDGE\u304c\u8868\u793a\u3055\u308c\u305f\u3089Array\u3092\u53d6\u308a\u51fa\u3057\u3001\u30ac\u30e9\u30b9\u9762\u306b\u6c17\u6ce1\u304c\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3001Septa\u306b\u30bf\u30d5\u30b9\u30dd\u30c3\u30c8\u3092\u8cbc\u308a\u307e\u3059\u3002\u3082\u3057\u3001\u6c17\u6ce1\u304c\u8a8d\u3081\u3089\u308c\u305f\u3089\u3001\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u3092\u4f7f\u3063\u3066Array Holding Buffer\u3092\u6c17\u6ce1\u304c\u5165\u3089\u306a\u3044\u3088\u3046\u306b\u5165\u308c\u76f4\u3057\u3066\u304b\u3089\u30bf\u30d5\u30b9\u30dd\u30c3\u30c8\u3092\u8cbc\u308a\u307e\u3059\u3002<br \/>\n7.GCC Scan Control<sup>\u203b<\/sup>\u3092\u8d77\u52d5\u3057\u3001Array\u306eScan\u3092\u958b\u59cb\u3057\u307e\u3059\u3002<br \/>\n\u203bAGCC Scan Control\u3068\u3082\u547c\u3073\u307e\u3059\u3002<br \/>\n8. Fluidics Station\u3092\u7d9a\u3051\u3066\u4f7f\u7528\u3059\u308b\u4e88\u5b9a\u304c\u306a\u3051\u308c\u3070\u3001Wash A\u3001Wash B\u306e\u30dc\u30c8\u30eb\u3092\u8d85\u7d14\u6c34\u306e\u5165\u3063\u305f\u30dc\u30c8\u30eb\u306b\u4ea4\u63db\u3057\u3001GCC Fluidics Control\u3067Shut Down\u3092\u884c\u3044\u307e\u3059\u3002Shut Down\u7d42\u4e86\u5f8c\u3001GCC Fluidics Control\u3092\u9589\u3058\u3001Fluidics Station\u306e\u96fb\u6e90\u3092\u5207\u308a\u307e\u3059\u3002<br \/>\n9. Array\u306eScan\u304c\u7d42\u4e86\u3057\u305f\u3089GCC Scan Control\u3092\u9589\u3058\u3001Scanner\u306e\u96fb\u6e90\u3092\u5207\u308a\u307e\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u95a2\u3059\u308b\u3054\u4e0d\u660e\u70b9\u3084\u6c17\u306b\u306a\u308b\u70b9\u306f<a href=\"mailto:jptech@thermofisher.com\">\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8<\/a>\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u4f7f\u7528\u3057\u305f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306f\u73fe\u5728\u3082\u3063\u3068\u3082\u30dd\u30d4\u30e5\u30e9\u30fc\u306a\u65b9\u6cd5\u3067\u3001\u767a\u73fe\u6bd4\u8f03\u7814\u7a76\u3084\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u63a2\u7d22\u306b\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u5b9f\u7e3e\u304c\u3042\u308a\u307e\u3059\u3002\u8fd1\u5e74\u3082\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u3084IncRNA\u89e3\u6790\u3092\u4e2d\u5fc3\u306b\u30a2\u30ec\u30a4\u306f\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304a\u308a<\/p>\n","protected":false},"author":975,"featured_media":9832,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[71],"division":[],"class_list":{"0":"post-9806","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-microarray","9":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb\" \/>\n<meta property=\"og:description\" content=\"\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u4f7f\u7528\u3057\u305f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306f\u73fe\u5728\u3082\u3063\u3068\u3082\u30dd\u30d4\u30e5\u30e9\u30fc\u306a\u65b9\u6cd5\u3067\u3001\u767a\u73fe\u6bd4\u8f03\u7814\u7a76\u3084\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u63a2\u7d22\u306b\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u5b9f\u7e3e\u304c\u3042\u308a\u307e\u3059\u3002\u8fd1\u5e74\u3082\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u3084IncRNA\u89e3\u6790\u3092\u4e2d\u5fc3\u306b\u30a2\u30ec\u30a4\u306f\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304a\u308a\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/\" \/>\n<meta property=\"og:site_name\" content=\"Learning at the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/ThermoFisherJapan\" \/>\n<meta property=\"article:published_time\" content=\"2021-08-13T08:07:29+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-08-26T08:03:20+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/shutterstock_1740294425-scaled.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"2560\" \/>\n\t<meta property=\"og:image:height\" content=\"1707\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"LatB Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:site\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"LatB Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"3\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/\"},\"author\":{\"name\":\"LatB Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/bdbe319a2001f279ddce6ab8eaad1253\"},\"headline\":\"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb\",\"datePublished\":\"2021-08-13T08:07:29+00:00\",\"dateModified\":\"2021-08-26T08:03:20+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/\"},\"wordCount\":525,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2021\\\/08\\\/shutterstock_1740294425-scaled.jpg\",\"keywords\":[\"\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\"],\"articleSection\":[\"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023\"],\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/\",\"name\":\"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb - Learning at the Bench\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2021\\\/08\\\/shutterstock_1740294425-scaled.jpg\",\"datePublished\":\"2021-08-13T08:07:29+00:00\",\"dateModified\":\"2021-08-26T08:03:20+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/bdbe319a2001f279ddce6ab8eaad1253\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/#breadcrumb\"},\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2021\\\/08\\\/shutterstock_1740294425-scaled.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2021\\\/08\\\/shutterstock_1740294425-scaled.jpg\",\"width\":2560,\"height\":1707},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\",\"name\":\"Learning at the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"ja\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/bdbe319a2001f279ddce6ab8eaad1253\",\"name\":\"LatB Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g\",\"caption\":\"LatB Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/lgbadmin\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb - Learning at the Bench","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/","og_locale":"ja_JP","og_type":"article","og_title":"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb","og_description":"\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u4f7f\u7528\u3057\u305f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306f\u73fe\u5728\u3082\u3063\u3068\u3082\u30dd\u30d4\u30e5\u30e9\u30fc\u306a\u65b9\u6cd5\u3067\u3001\u767a\u73fe\u6bd4\u8f03\u7814\u7a76\u3084\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u63a2\u7d22\u306b\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u5b9f\u7e3e\u304c\u3042\u308a\u307e\u3059\u3002\u8fd1\u5e74\u3082\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u3084IncRNA\u89e3\u6790\u3092\u4e2d\u5fc3\u306b\u30a2\u30ec\u30a4\u306f\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304a\u308a","og_url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/","og_site_name":"Learning at the Bench","article_publisher":"https:\/\/www.facebook.com\/ThermoFisherJapan","article_published_time":"2021-08-13T08:07:29+00:00","article_modified_time":"2021-08-26T08:03:20+00:00","og_image":[{"width":2560,"height":1707,"url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/shutterstock_1740294425-scaled.jpg","type":"image\/jpeg"}],"author":"LatB Staff","twitter_card":"summary_large_image","twitter_creator":"@ThermoFisherJP","twitter_site":"@ThermoFisherJP","twitter_misc":{"\u57f7\u7b46\u8005":"LatB Staff","\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593":"3\u5206"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/"},"author":{"name":"LatB Staff","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/bdbe319a2001f279ddce6ab8eaad1253"},"headline":"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb","datePublished":"2021-08-13T08:07:29+00:00","dateModified":"2021-08-26T08:03:20+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/"},"wordCount":525,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/shutterstock_1740294425-scaled.jpg","keywords":["\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4"],"articleSection":["\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"],"inLanguage":"ja","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/","url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/","name":"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb - Learning at the Bench","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/shutterstock_1740294425-scaled.jpg","datePublished":"2021-08-13T08:07:29+00:00","dateModified":"2021-08-26T08:03:20+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/bdbe319a2001f279ddce6ab8eaad1253"},"breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/#breadcrumb"},"inLanguage":"ja","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/"]}]},{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/shutterstock_1740294425-scaled.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/shutterstock_1740294425-scaled.jpg","width":2560,"height":1707},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wtpicoreagentkit_protocol_ma_gsd_mkt_1\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/"},{"@type":"ListItem","position":2,"name":"WT Pico Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/","name":"Learning at the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"ja"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/bdbe319a2001f279ddce6ab8eaad1253","name":"LatB Staff","image":{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/secure.gravatar.com\/avatar\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g","caption":"LatB Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/lgbadmin\/"}]}},"taxonomy_info":{"category":[{"value":156,"label":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"}],"post_tag":[{"value":71,"label":"\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/shutterstock_1740294425-1024x683.jpg",760,507,true],"author_info":{"display_name":"LatB Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/lgbadmin\/"},"comment_info":0,"category_info":[{"term_id":156,"name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","slug":"mol-bio","term_group":0,"term_taxonomy_id":156,"taxonomy":"category","description":"","parent":0,"count":538,"filter":"raw","meta":[],"cat_ID":156,"category_count":538,"category_description":"","cat_name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","category_nicename":"mol-bio","category_parent":0}],"tag_info":[{"term_id":71,"name":"\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4","slug":"microarray","term_group":0,"term_taxonomy_id":71,"taxonomy":"post_tag","description":"","parent":0,"count":28,"filter":"raw","meta":[]}],"jetpack-related-posts":[{"id":20152,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-nucleic-acid-purification-ts-r-25017\/","url_meta":{"origin":9806,"position":0},"title":"\u3010\u7b2c3\u56de\u3011\u201cRNA cargo\u201d\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790","author":"LatB Staff","date":"2026\u5e745\u670813\u65e5","format":false,"excerpt":"\u7b2c2\u56de\u3067\u306f\u3001\u57f9\u990a\u4e0a\u6e05\u3084\u3055\u307e\u3056\u307e\u306a\u4f53\u6db2\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u306e\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\u306e\u6fc3\u7e2e\u30fb\u56de\u53ce\u65b9\u6cd5\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u307e\u3057\u305f\u304c\u2026","rel":"","context":"\u305d\u306e\u4ed6","block_context":{"text":"\u305d\u306e\u4ed6","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/else\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/f17931341a23d82235760ed66ded9c41.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/f17931341a23d82235760ed66ded9c41.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/f17931341a23d82235760ed66ded9c41.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/f17931341a23d82235760ed66ded9c41.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/f17931341a23d82235760ed66ded9c41.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/f17931341a23d82235760ed66ded9c41.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":25325,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-gene-expression-ts-r-25019\/","url_meta":{"origin":9806,"position":1},"title":"\u3010\u7b2c5\u56de\u3011 &#8220;RNA cargo&#8221;\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\u300d\uff5emRNA\u306e\u5b9a\u91cf\u89e3\u6790\uff5e","author":"LatB Staff","date":"2026\u5e746\u670811\u65e5","format":false,"excerpt":"\u4eca\u56de\u306f\u3001\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\uff08\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09\u4e2d\u306emRNA\u306e\u5b9a\u91cf\u89e3\u6790\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002\u7b2c3\u56de\u3067\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\u304b\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":25247,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-gene-expression-ts-r-25018\/","url_meta":{"origin":9806,"position":2},"title":"\u3010\u7b2c4\u56de\u3011&#8221;RNA cargo&#8221;\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\uff5emiRNA\u306e\u5b9a\u91cf\u89e3\u6790\uff5e","author":"LatB Staff","date":"2026\u5e745\u670829\u65e5","format":false,"excerpt":"\u524d\u56de\uff08\u3010\u7b2c3\u56de\u3011\u201cRNA cargo\u201d\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\uff09\u3067\u306f\u3001\u30a8\u30af\u30bd\u30bd\u30fc\u30e0 \uff08\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09 \u304b\u3089\u306e\u2026","rel":"","context":"\u5206\u6790\u30fb\u6e2c\u5b9a\u95a2\u9023","block_context":{"text":"\u5206\u6790\u30fb\u6e2c\u5b9a\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/analysis\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":24187,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ngs-gene-expression-csd-ts-ion-25073\/","url_meta":{"origin":9806,"position":3},"title":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u5fae\u91cf\u30fb\u5206\u89e3RNA\u306b\u3088\u308b\u767a\u73fe\u89e3\u6790\u306e\u6700\u9069\u89e3\u306f\uff1fRNA-Seq\u3068Ion AmpliSeq Transcriptome\u3092\u6bd4\u3079\u3066\u307f\u305f","author":"LatB Staff","date":"2025\u5e7411\u670828\u65e5","format":false,"excerpt":"\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u306e\u767a\u73fe\u89e3\u6790\u306b\u53d6\u308a\u7d44\u3080\u969b\u3001RNA\u30b5\u30f3\u30d7\u30eb\u304c\u300c\u5c11\u306a\u3059\u304e\u308b\u300d\u300c\u5206\u89e3\u3057\u3066\u3044\u308b\u300d\u3068\u3044\u3046\u72b6\u6cc1\u306f\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/11\/ngs-gene-expression-csd-ts-ion-25073_eyecatch-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":24953,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ngs-gene-expression-csd-ts-ion-26011\/","url_meta":{"origin":9806,"position":4},"title":"\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u306e\u81ea\u52d5\u5316\u3067NGS\u306e\u53ef\u80fd\u6027\u3092\u5e83\u3052\u308b\u2015Ion Chef\u30b7\u30b9\u30c6\u30e0\u304c\u5909\u3048\u308b\u7814\u7a76\u5ba4\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc","author":"LatB Staff","date":"2026\u5e744\u670810\u65e5","format":false,"excerpt":"\u524d\u51e6\u7406\u306e\u81ea\u52d5\u5316\u304c\u3001NGS\u306e\u65b0\u3057\u3044\u6a19\u6e96\u3092\u3064\u304f\u308b\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\uff08NGS\uff09\u304c\u65e5\u5e38\u7684\u306a\u7814\u7a76\u30c4\u30fc\u30eb\u3068\u306a\u3063\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/02\/ngs-gene-expression-csd-ts-ion-26011_eyecatch.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/02\/ngs-gene-expression-csd-ts-ion-26011_eyecatch.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/02\/ngs-gene-expression-csd-ts-ion-26011_eyecatch.jpeg?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":25565,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-antibody-ts-r-25068_\/","url_meta":{"origin":9806,"position":5},"title":"\u3010\u7b2c8\u56de\u3011\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\u30b5\u30d6\u30bf\u30a4\u30d7\u306e\u5206\u753b\u6cd5 \uff1a \u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\uff0fqRT-PCR\uff0f\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306b\u9069\u3057\u305f\u5206\u753b\u65b9\u6cd5","author":"LatB Staff","date":"2026\u5e749\u670829\u65e5","format":false,"excerpt":"\u672c\u7a3f\u3067\u306f\u3001\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\u819c\u4e0a\u306e\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6a19\u7684\u3068\u3057\u305f\u514d\u75ab\u5438\u7740\u6cd5\u306b\u57fa\u3065\u304f\u5206\u753b\u624b\u6cd5\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002\u30a8\u30af\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25019_eyecatch.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"amp_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2021\/08\/shutterstock_1740294425-scaled.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/9806","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/users\/975"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/comments?post=9806"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/9806\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media\/9832"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media?parent=9806"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/categories?post=9806"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/tags?post=9806"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/division?post=9806"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}