Optimize mAb Production with DynaSpin Single-Use Centrifuge

In the competitive field of bioprocessing, optimizing the efficiency and yield of mAb production is crucial. Monoclonal antibodies are vital in treating various diseases, making their efficient production a significant focus in the biopharmaceutical industry. This application note explores the optimization of the Thermo Scientific DynaSpin Single-Use Centrifuge for cell culture harvests, highlighting how adjusting operational parameters such as rotor speed and flow rate can significantly impact separation efficiency, product yield, and overall process costs.

Learn how to maximize efficiency and yield with this app note

Key findings

The study investigated the impact of varying flow rates and rotor speeds on the performance of the DynaSpin Centrifuge. The primary objectives were to:

• Optimize separation efficiency
• Maximize product yield
• Minimize operational costs

Optimization of operational parameters

Adjusting the flow rate and rotor speed demonstrated a significant effect on the centrifuge’s performance. Lower flow rates were found to maximize separation efficiency but increase processing time. Conversely, higher flow rates reduced processing time but led to increased cell lysis and turbidity in the centrate.

Impact on cell lysis and clarification

Higher flow rates resulted in greater cell lysis, which negatively impacted the quality of the harvested product. This finding underscores the importance of balancing flow rate adjustments to maintain product integrity.

Economic and sustainability considerations

The study also highlighted the economic trade-offs involved in the optimization process. By fine-tuning operational parameters, the number of filters required post-centrifugation was reduced, leading to lower material and labor costs. Additionally, optimizing these parameters contributed to sustainability efforts by decreasing water and plastic waste.