Cell culture forms the backbone of many scientific discoveries and advancements in various fields, from genetics to drug discovery. The ability to accurately monitor and control the carbon dioxide (CO2) levels in a cell culture incubator is a critical factor in maintaining optimal culture conditions. With the advent of innovative sensor technologies, the ability to mimic mammalian physiological pH has become increasingly sophisticated and efficient.
Traditional infrared (IR) CO2 sensors are widely used in the industry. However, they come with their set of challenges. The incandescent light sources that are commonly used in these sensors tend to burn out over time, leading to inaccurate readings. Furthermore, dust and dirt can accumulate on the sensor surfaces, causing interference and apparent changes in CO2 concentration. Most of these sensors are positioned outside the incubation chamber, which can result in a delayed response to changes within the chamber.
In response to these challenges, advancements in sensor technology have led to the development of more robust and durable CO2 sensors, such as the precision sensors found in various Thermo Scientific CO2 Incubators. These next-generation sensors utilize silicon micro-electrical mechanical systems (MEMS) emitters, which are not only more robust but also have a significantly longer lifespan than their incandescent counterparts. This translates into substantial cost savings due to reduced calibration and replacement needs.
These advanced sensors are positioned in the incubation chamber to react to the same conditions that the cells experience. This positioning leads to more accurate and immediate measurements. Moreover, the design of these sensors allows them to withstand multiple high-temperature sterilization cycles, significantly reducing the risk of contamination.
In terms of operation, these sensors employ a single beam with dual wavelengths and a micromachined Fabry-Perot Interferometer (FPI) filter in front of a single detector. This unique design compensates for changes in the IR light intensity and the accumulation of dirt and dust, without the need for a second detector.
In tests, these advanced CO2 sensors have shown exceptional stability over time and under different CO2 concentrations. They remain well within prescribed specifications, even after withstanding numerous high-temperature sterilization cycles.
In conclusion, the advancements in infrared CO2 sensor technology offer a highly accurate, stable, and durable solution for CO2 monitoring in cell culture incubators. To dive deeper into the world of IR sensors, read our application note, “Evaluating Infrared Carbon Dioxide Sensors for 21st Century Cell Culture: Introducing the Thermo Scientific IR180Si Infrared CO2 Sensor,” and learn how IR sensors are transforming the cell culture landscape.
Visit our CO2 incubator selection guide to filter by CO2 sensor and determine which model is the best fit for your lab needs.
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