{"id":7657,"date":"2016-02-19T07:01:12","date_gmt":"2016-02-19T12:01:12","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/?p=7657"},"modified":"2016-04-29T16:52:39","modified_gmt":"2016-04-29T16:52:39","slug":"hyperlopit-for-the-mouse-stem-cell-proteome","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/","title":{"rendered":"HyperLOPIT for the Mouse Stem Cell Proteome"},"content":{"rendered":"

\"MouseWhen researchers want to know more about <\/span><\/span>pluripotency<\/span><\/span> and the push towards stem cell differentiation, they need to know more than just which genes are firing up<\/span>. <\/span><\/span>Christoforou<\/span><\/span> et al. (2016) suggest that they also need to know what proteins are on the go, and furthermore, where they are <\/span><\/span>spatially<\/span><\/span> <\/span><\/span>located<\/span><\/span> within the cell.<\/span><\/span>1<\/span><\/span><\/sup> <\/span><\/span> <\/span><\/p>\n

The researchers propose an upgrade of a technique used to determine spatial proteomics: <\/span><\/span>hyperLOPIT<\/span><\/span>, which stands for a high-throughput <\/span><\/span>mass<\/span><\/span> spectrometric analysis of the products of localization of organelle proteins by isotope tagging (LOPIT). Using a combination of density ultracentrifugation, sample multiplexing and a liquid chromatography–mass spectrometry (LC-MS) proteomics approach, the new workflow can identify proteins and pinpoint location with organelles or sub-organelle fractions.<\/span><\/span> <\/span><\/p>\n

The research team used the pluripotent mouse embryonic stem cell line E14TG2A for the experiments. First<\/span><\/span>,<\/span><\/span> they lysed the cells using a detergent-free system that would preserve organelle structure with minimal disruption to contents. They then subjected the lysate to extensive fractionation, using density gradient ultracentrifugation. <\/span><\/span>Since each membrane and organelle group within a cell displays distinct enrichment patterns, t<\/span><\/span>his step separated <\/span><\/span>them<\/span><\/span> into <\/span><\/span>fractions that could be prepared individually for subsequent MS evaluation.<\/span><\/span> <\/span><\/p>\n

Following fractionation, the researchers chose 10 fractions for MS analysis. First, they digested the fractionated lysates using trypsin and then labeled the proteolytic digests using isobaric <\/span><\/span>tandem <\/span><\/span>mass<\/span><\/span> tagging (TMT)<\/span><\/span><\/span><\/a> techniques (<\/span><\/span>Thermo<\/span><\/span> Scientific). Using the m<\/span><\/span>ost recent<\/span><\/span> TMT technology available, this meant that the digests <\/span><\/span>could be multiplexed<\/span><\/span> to a 10-plex analytical preparation, thus maximizing data forthcoming from the cell studies.<\/span><\/span> <\/span><\/p>\n

Christoforou<\/span><\/span> et al. initially focused on optimizing <\/span><\/span>the LC-MS analytical workflow, using a <\/span><\/span>Proxeon<\/span><\/span> Easy-<\/span><\/span>nLC<\/span><\/span><\/a> 1000<\/a> system in combination with an <\/span><\/span>Orbitrap<\/span><\/span> Fusion <\/span><\/span>Tribrid<\/span><\/span><\/a> mass spectrometer (both <\/span><\/span>Thermo<\/span><\/span> Scientific). In order to collect as many peptide identities as possible, the team looked to synchronous precursor selection (SPS) with MS3 instrument operation. They found that using an SPS approach<\/span><\/span> <\/span><\/span>balance<\/span><\/span>d<\/span><\/span> the gains in quantitation brought by MS3 operation with the sensitivity required for peptide identification.<\/span><\/span> Furthermore, by increasing the frequency notches in the isolation waveforms, they could enhance signals from TMT reporter ions and thus improve organelle resolution in addition to boosting the accuracy of the quantitation data gathered. Although the number of peptide spectral matches <\/span><\/span>decreased from 137,912 to 6<\/span><\/span>1,090<\/span><\/span> with SPS-MS3, the team considered that the approach brought benefits over MS2 data acquisition, since they achieved an acceptable number of quantifiable protein groups (7,114 for MS2 vs. 5,489 for SPS-MS3).<\/span><\/span> <\/span><\/p>\n

Once the team optimized the workflow and r<\/span><\/span>a<\/span><\/span>n the experimental replicates,<\/span><\/span> they analyzed the data <\/span><\/span>u<\/span><\/span>s<\/span><\/span>ing Proteome Discover<\/a> software <\/span>v1.4 for analysis (<\/span><\/span>Thermo<\/span><\/span> Scientific). They explored <\/span><\/span>manual localization of protein identities against a constructed software algorithm for determining spatial identity. From the spectral <\/span><\/span>data<\/span><\/span> they found that fewer than 5% of proteins were assigned to contradictory locations within the cell. Overall, the workflow and subsequent analysis located 2,855 proteins with 14 organelles and sub-organelles, giving new spatial data for around 350 proteins. This represented more than 50% of the proteins identified during the SPS-MS3 run. Of the remainder, <\/span><\/span>Christoforou<\/span><\/span> et al. found that these located to cytoskeletal elements or multiple compartments, or were in transit<\/span><\/span>.<\/span><\/span> <\/span><\/p>\n

In conclu<\/span><\/span>sion<\/span><\/span>, the authors believe strongly that the workflow and methodology presented are valuable tools for investigating organelle structure, protein complexes and functional networks within cells. Furthermore, they suggest that <\/span><\/span>hyperLOPIT<\/span><\/span> is also useful for determining protein isoform localization and looking at other post-transcriptional events important as regulators of differentiation in pluripotent stem cells.<\/span><\/span> <\/span><\/p>\n

<\/span><\/span> <\/span><\/p>\n

The software behind <\/span><\/span>hyperLOPIT<\/span><\/span> data analysis is available as an <\/span>Open Source resource<\/a> for visualizing and annotating spatial proteomics<\/span><\/span> <\/span><\/span>data.<\/span><\/span> <\/span><\/span> <\/span><\/span><\/p>\n

<\/span><\/span> <\/span><\/p>\n

Reference<\/span><\/span><\/strong> <\/span><\/p>\n

1. Christoforou<\/span>, A., et al. (2016) “<\/span>A draft map of the mouse pluripotent stem cell spatial proteome<\/a>,<\/span><\/span>” <\/span><\/span>Nature Communications, 7(8992), <\/span><\/span>doi: 10.1038\/ncomms9992.<\/span><\/span> <\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

When researchers want to know more about pluripotency and the push towards stem cell differentiation, they need to know more than just which genes are firing up. Christoforou et al. (2016) suggest that they also need to know what proteins are on the go, and furthermore, where they are spatially located within the cell.1   The researchers propose an upgrade of a technique<\/p>\n","protected":false},"author":21,"featured_media":7733,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[12],"tags":[824,825,598],"division":[],"class_list":{"0":"post-7657","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-methods","8":"tag-hyperlopit","9":"tag-spatial-proteome","10":"tag-stem-cells","11":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\nHyperLOPIT for the Mouse Stemcell Proteome<\/title>\n<meta name=\"description\" content=\"Using HyperLOPIT for spatial proteomics determines a draft mouse stem cell proteome that shows the regulation of differentiation from pluripotency.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"HyperLOPIT for the Mouse Stem Cell Proteome\" \/>\n<meta property=\"og:description\" content=\"Using HyperLOPIT for spatial proteomics determines a draft mouse stem cell proteome that shows the regulation of differentiation from pluripotency.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/\" \/>\n<meta property=\"og:site_name\" content=\"Accelerating Proteomics\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2016-02-19T12:01:12+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2016-04-29T16:52:39+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/02\/mouse_embryonic_stem_cells1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"507\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Amanda Maxwell\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Amanda Maxwell\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/\"},\"author\":{\"name\":\"Amanda Maxwell\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#\\\/schema\\\/person\\\/f572b16973d4ff71460b75a0a41a3632\"},\"headline\":\"HyperLOPIT for the Mouse Stem Cell Proteome\",\"datePublished\":\"2016-02-19T12:01:12+00:00\",\"dateModified\":\"2016-04-29T16:52:39+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/\"},\"wordCount\":691,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/wp-content\\\/uploads\\\/sites\\\/2\\\/2016\\\/02\\\/mouse_embryonic_stem_cells1.jpg\",\"keywords\":[\"hyperLOPIT\",\"spatial proteome\",\"stem cells\"],\"articleSection\":[\"Methods\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/\",\"name\":\"HyperLOPIT for the Mouse Stemcell Proteome\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/wp-content\\\/uploads\\\/sites\\\/2\\\/2016\\\/02\\\/mouse_embryonic_stem_cells1.jpg\",\"datePublished\":\"2016-02-19T12:01:12+00:00\",\"dateModified\":\"2016-04-29T16:52:39+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#\\\/schema\\\/person\\\/f572b16973d4ff71460b75a0a41a3632\"},\"description\":\"Using HyperLOPIT for spatial proteomics determines a draft mouse stem cell proteome that shows the regulation of differentiation from pluripotency.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/wp-content\\\/uploads\\\/sites\\\/2\\\/2016\\\/02\\\/mouse_embryonic_stem_cells1.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/wp-content\\\/uploads\\\/sites\\\/2\\\/2016\\\/02\\\/mouse_embryonic_stem_cells1.jpg\",\"width\":600,\"height\":507,\"caption\":\"Mouse embryonic stem cells. Image: Public Domain, https:\\\/\\\/commons.wikimedia.org\\\/w\\\/index.php?curid=456457\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/hyperlopit-for-the-mouse-stem-cell-proteome\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"HyperLOPIT for the Mouse Stem Cell Proteome\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/\",\"name\":\"Accelerating Proteomics\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#\\\/schema\\\/person\\\/f572b16973d4ff71460b75a0a41a3632\",\"name\":\"Amanda Maxwell\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/80b1d135aff6be035b6cc16276a4d7f56aa67c117044edb6d87067d0ffa40ae5?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/80b1d135aff6be035b6cc16276a4d7f56aa67c117044edb6d87067d0ffa40ae5?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/80b1d135aff6be035b6cc16276a4d7f56aa67c117044edb6d87067d0ffa40ae5?s=96&d=mm&r=g\",\"caption\":\"Amanda Maxwell\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/amandamaxwell\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"HyperLOPIT for the Mouse Stemcell Proteome","description":"Using HyperLOPIT for spatial proteomics determines a draft mouse stem cell proteome that shows the regulation of differentiation from pluripotency.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/","og_locale":"en_US","og_type":"article","og_title":"HyperLOPIT for the Mouse Stem Cell Proteome","og_description":"Using HyperLOPIT for spatial proteomics determines a draft mouse stem cell proteome that shows the regulation of differentiation from pluripotency.","og_url":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/","og_site_name":"Accelerating Proteomics","article_publisher":"https:\/\/www.facebook.com\/thermofisher","article_published_time":"2016-02-19T12:01:12+00:00","article_modified_time":"2016-04-29T16:52:39+00:00","og_image":[{"width":600,"height":507,"url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/02\/mouse_embryonic_stem_cells1.jpg","type":"image\/jpeg"}],"author":"Amanda Maxwell","twitter_card":"summary_large_image","twitter_creator":"@thermofisher","twitter_site":"@thermofisher","twitter_misc":{"Written by":"Amanda Maxwell","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/"},"author":{"name":"Amanda Maxwell","@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#\/schema\/person\/f572b16973d4ff71460b75a0a41a3632"},"headline":"HyperLOPIT for the Mouse Stem Cell Proteome","datePublished":"2016-02-19T12:01:12+00:00","dateModified":"2016-04-29T16:52:39+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/"},"wordCount":691,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/02\/mouse_embryonic_stem_cells1.jpg","keywords":["hyperLOPIT","spatial proteome","stem cells"],"articleSection":["Methods"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/","url":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/","name":"HyperLOPIT for the Mouse Stemcell Proteome","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/02\/mouse_embryonic_stem_cells1.jpg","datePublished":"2016-02-19T12:01:12+00:00","dateModified":"2016-04-29T16:52:39+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#\/schema\/person\/f572b16973d4ff71460b75a0a41a3632"},"description":"Using HyperLOPIT for spatial proteomics determines a draft mouse stem cell proteome that shows the regulation of differentiation from pluripotency.","breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/02\/mouse_embryonic_stem_cells1.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/02\/mouse_embryonic_stem_cells1.jpg","width":600,"height":507,"caption":"Mouse embryonic stem cells. Image: Public Domain, https:\/\/commons.wikimedia.org\/w\/index.php?curid=456457"},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/hyperlopit-for-the-mouse-stem-cell-proteome\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/proteomics\/"},{"@type":"ListItem","position":2,"name":"HyperLOPIT for the Mouse Stem Cell Proteome"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#website","url":"https:\/\/admin.acceleratingscience.com\/proteomics\/","name":"Accelerating Proteomics","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/proteomics\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#\/schema\/person\/f572b16973d4ff71460b75a0a41a3632","name":"Amanda Maxwell","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/80b1d135aff6be035b6cc16276a4d7f56aa67c117044edb6d87067d0ffa40ae5?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/80b1d135aff6be035b6cc16276a4d7f56aa67c117044edb6d87067d0ffa40ae5?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/80b1d135aff6be035b6cc16276a4d7f56aa67c117044edb6d87067d0ffa40ae5?s=96&d=mm&r=g","caption":"Amanda Maxwell"},"url":"https:\/\/admin.acceleratingscience.com\/author\/amandamaxwell\/"}]}},"jetpack-related-posts":[],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/02\/mouse_embryonic_stem_cells1.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/posts\/7657","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/users\/21"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/comments?post=7657"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/posts\/7657\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/media\/7733"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/media?parent=7657"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/categories?post=7657"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/tags?post=7657"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/division?post=7657"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}