{"id":8870,"date":"2016-10-10T22:44:29","date_gmt":"2016-10-10T22:44:29","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/proteomics\/?p=8870"},"modified":"2016-10-10T22:44:29","modified_gmt":"2016-10-10T22:44:29","slug":"digging-deep-single-cell-proteomics-of-tomato-root-tips","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/","title":{"rendered":"Digging Deep: Single-Cell Proteomics of Tomato Root Tips"},"content":{"rendered":"<p><span><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.thermofisher.com\/blog\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/10\/shutterstock_214525762-1.jpg\" style=\"float: left;margin: 5px\" alt=\"Bunch of red tomato with rain drops on a tree. Image: lobsters\/Shutterstock.com.\" width=\"330\" height=\"220\" \/>Single-cell-type proteomics is helpful for studying plant roots, which can contain several populations of unique cells. These cell populations provide information regarding root development and plasticity under various conditions. Researchers Zhu et al. report on a new application of single-cell proteomics using laser capture microdissection (LCM) in tomato seedlings grown in hydroponic tanks.<sup>1 <\/sup><\/span><\/p>\n<p>The team treated half of the samples with 14.5 &mu;M Al<sup>3+<\/sup> solution to simulate the effects of aluminum&nbsp;toxicity in soil. They harvested the seedlings 10 days after seed germination, at the point when the two cotyledons&nbsp;<span class=\"thread\">enlarged <\/span>and lateral roots began to grow on some of the Al-treated plants. They&nbsp;<span class=\"thread\">then fixed<\/span>&nbsp;the roots and embedded them in optimal cutting temperature (OCT) compound. Using a cryostat, the team collected thin sections, approximately 10-&mu;m thick, with 10 sections per root tip. They mounted the sections onto <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/LCM0522\" target=\"_blank\"><span>polyethylene naphthalate (PEN) membrane glass slides<\/span><\/a> (Thermo Scientific) at &minus;20&deg;C.<\/p>\n<p><span>Using the LCM microscope, the team harvested epidermal and cortical cells (5,000&ndash;7,000 cells per tissue type). The LCM cut the single-layer tissues, <\/span><span>lifted them from the slides and collected the sample into a capture tube. <\/span><span>They then isolated proteins and separated them using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by an in-gel tryptic digestion and <\/span><span>nano liquid chromatography&ndash;tandem mass spectrometry (LC-MS\/MS) <\/span><span>analysis.<\/span><\/p>\n<p><span>Zhu and colleagues used an <\/span><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/IQLAAEGAAPFADBMAZQ\" target=\"_blank\"><span>Orbitrap Elite mass spectrometer<\/span><\/a><span> (Thermo Scientific) to serially analyze&nbsp;10 or 11 individually digested gel fractions.They also used an <\/span><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/IQLAAEGAAPFADBMBCX?ICID=search-product\" target=\"_blank\"><span>Orbitrap Fusion Tribrid mass spectrometer<\/span><\/a><span> (Thermo Scientific) to analyze epidermal samples pooled from five separately digested gel fractions, to avoid the need for pre-fractionation while also allowing for a more rapid scanning rate. Both of the mass spectrometers <span class=\"thread\">were coupled<\/span> with an <\/span><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/ULTIM3000RSLCNANO\" target=\"_blank\"><span>UltiMate 3000 RSLCnano<\/span><\/a><span> (Thermo Scientific), using the same LC protocol. After generating MS spectra, the team used <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/IQLAAEGABSFAKJMAUH\" target=\"_blank\"><span class=\"thread\">Proteome Discoverer software<\/span><\/a>, revision 1.4, (Thermo Scientific) to process raw data and then matched the spectra against the ITAG2.4 tomato genome database to identify proteins contained in each single-cell-type sample. <\/span><\/p>\n<p><span>The researchers identified 1,313 proteins in the Al-treated epidermal cell population and 744 proteins in the Al-treated cortical cell population. They identified 365 proteins in the non-Al-treated epidermal cell population<span class=\"thread\"> and 745 proteins <\/span>in the non-Al-treated cortical cell population. Between the Al-treated epidermal and cortical proteomes, 543 proteins overlapped in these two layers of tissues. Between the non-treated epidermal and cortical proteomes, 189 proteins overlapped in these two types of tissues. <span class=\"thread\">In both cases,<span> they found a significant portion of the proteome&nbsp;<span class=\"thread\">contained&nbsp;<\/span>proteins unique to the different layers and cell type.<\/span> <\/span>Another unique finding was the identification of several important proteins&nbsp;<span class=\"thread\">related<\/span> to Al-induced morphological characteristics of roots. These proteins include the ROOT GROWTH DEFECTIVE 3; the ROOT HAIRLESS 1, required for root hair initiation in <\/span><span>Arabidopsis;<\/span><span> and the DEFORMED ROOTS AND LEAVES 1 for short root phenotypes, in the epidermal proteins.<\/span><\/p>\n<p><span>These results demonstrate the cell-type-specific proteome is relevant for tissue-specific functions in tomato roots. The authors also note that this technique is still in its infancy with plenty of room for improvement. <\/span><\/p>\n<p><span><strong>Reference<\/strong><br \/>1. <span>Zhu, Y., et al. (2016) &ldquo;<\/span><a href=\"http:\/\/www.nature.com\/articles\/hortres201626\" target=\"_blank\"><span>Development of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots<\/span><\/a><span>,&rdquo; Horticulture Research, 3, doi:10.1038\/hortres.2016.26.<\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Single-cell-type proteomics is helpful for studying plant roots, which can contain several populations of unique cells. These cell populations provide information regarding root development and plasticity under various conditions. Researchers Zhu et al. report on a new application of single-cell proteomics using laser capture microdissection (LCM) in tomato seedlings grown in hydroponic tanks.1 The team<\/p>\n","protected":false},"author":13,"featured_media":8881,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[12,24],"tags":[176,214],"division":[],"class_list":{"0":"post-8870","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-methods","8":"category-non-human-proteomics","9":"tag-lc-msms","10":"tag-protein-identification","11":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Digging Deep: Single-Cell Proteomics of Tomato Root Tips<\/title>\n<meta name=\"description\" content=\"Researchers Zhu et al. report on a new application of single-cell proteomics using laser capture microdissection (LCM) in tomato seedlings grown in hydroponic tanks.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Digging Deep: Single-Cell Proteomics of Tomato Root Tips\" \/>\n<meta property=\"og:description\" content=\"Researchers Zhu et al. report on a new application of single-cell proteomics using laser capture microdissection (LCM) in tomato seedlings grown in hydroponic tanks.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/\" \/>\n<meta property=\"og:site_name\" content=\"Accelerating Proteomics\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2016-10-10T22:44:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/10\/shutterstock_214525762-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"500\" \/>\n\t<meta property=\"og:image:height\" content=\"334\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Emily Humphreys\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Emily Humphreys\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/\"},\"author\":{\"name\":\"Emily Humphreys\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#\\\/schema\\\/person\\\/d44674a977ab12b9f2506bec3843973b\"},\"headline\":\"Digging Deep: Single-Cell Proteomics of Tomato Root Tips\",\"datePublished\":\"2016-10-10T22:44:29+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/\"},\"wordCount\":540,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/wp-content\\\/uploads\\\/sites\\\/2\\\/2016\\\/10\\\/shutterstock_214525762-1.jpg\",\"keywords\":[\"LC-MS\\\/MS\",\"protein identification\"],\"articleSection\":[\"Methods\",\"Non-Human Proteomics\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/\",\"name\":\"Digging Deep: Single-Cell Proteomics of Tomato Root Tips\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/wp-content\\\/uploads\\\/sites\\\/2\\\/2016\\\/10\\\/shutterstock_214525762-1.jpg\",\"datePublished\":\"2016-10-10T22:44:29+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#\\\/schema\\\/person\\\/d44674a977ab12b9f2506bec3843973b\"},\"description\":\"Researchers Zhu et al. report on a new application of single-cell proteomics using laser capture microdissection (LCM) in tomato seedlings grown in hydroponic tanks.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/wp-content\\\/uploads\\\/sites\\\/2\\\/2016\\\/10\\\/shutterstock_214525762-1.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/wp-content\\\/uploads\\\/sites\\\/2\\\/2016\\\/10\\\/shutterstock_214525762-1.jpg\",\"width\":500,\"height\":334,\"caption\":\"Bunch of red tomato with rain drops on a tree. Image: lobsters\\\/Shutterstock.com.\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/proteomics\\\/digging-deep-single-cell-proteomics-of-tomato-root-tips\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Digging Deep: Single-Cell Proteomics of Tomato Root Tips\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/\",\"name\":\"Accelerating Proteomics\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/proteomics\\\/#\\\/schema\\\/person\\\/d44674a977ab12b9f2506bec3843973b\",\"name\":\"Emily Humphreys\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/878cbec1206c605e58941f79404bd506da2c188f011046a339d0b069c1c97d0d?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/878cbec1206c605e58941f79404bd506da2c188f011046a339d0b069c1c97d0d?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/878cbec1206c605e58941f79404bd506da2c188f011046a339d0b069c1c97d0d?s=96&d=mm&r=g\",\"caption\":\"Emily Humphreys\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/emilyhumphreys\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Digging Deep: Single-Cell Proteomics of Tomato Root Tips","description":"Researchers Zhu et al. report on a new application of single-cell proteomics using laser capture microdissection (LCM) in tomato seedlings grown in hydroponic tanks.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/","og_locale":"en_US","og_type":"article","og_title":"Digging Deep: Single-Cell Proteomics of Tomato Root Tips","og_description":"Researchers Zhu et al. report on a new application of single-cell proteomics using laser capture microdissection (LCM) in tomato seedlings grown in hydroponic tanks.","og_url":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/","og_site_name":"Accelerating Proteomics","article_publisher":"https:\/\/www.facebook.com\/thermofisher","article_published_time":"2016-10-10T22:44:29+00:00","og_image":[{"width":500,"height":334,"url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/10\/shutterstock_214525762-1.jpg","type":"image\/jpeg"}],"author":"Emily Humphreys","twitter_card":"summary_large_image","twitter_creator":"@thermofisher","twitter_site":"@thermofisher","twitter_misc":{"Written by":"Emily Humphreys","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/"},"author":{"name":"Emily Humphreys","@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#\/schema\/person\/d44674a977ab12b9f2506bec3843973b"},"headline":"Digging Deep: Single-Cell Proteomics of Tomato Root Tips","datePublished":"2016-10-10T22:44:29+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/"},"wordCount":540,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/10\/shutterstock_214525762-1.jpg","keywords":["LC-MS\/MS","protein identification"],"articleSection":["Methods","Non-Human Proteomics"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/","url":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/","name":"Digging Deep: Single-Cell Proteomics of Tomato Root Tips","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/10\/shutterstock_214525762-1.jpg","datePublished":"2016-10-10T22:44:29+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#\/schema\/person\/d44674a977ab12b9f2506bec3843973b"},"description":"Researchers Zhu et al. report on a new application of single-cell proteomics using laser capture microdissection (LCM) in tomato seedlings grown in hydroponic tanks.","breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/10\/shutterstock_214525762-1.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/10\/shutterstock_214525762-1.jpg","width":500,"height":334,"caption":"Bunch of red tomato with rain drops on a tree. Image: lobsters\/Shutterstock.com."},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/digging-deep-single-cell-proteomics-of-tomato-root-tips\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/proteomics\/"},{"@type":"ListItem","position":2,"name":"Digging Deep: Single-Cell Proteomics of Tomato Root Tips"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#website","url":"https:\/\/admin.acceleratingscience.com\/proteomics\/","name":"Accelerating Proteomics","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/proteomics\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/proteomics\/#\/schema\/person\/d44674a977ab12b9f2506bec3843973b","name":"Emily Humphreys","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/878cbec1206c605e58941f79404bd506da2c188f011046a339d0b069c1c97d0d?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/878cbec1206c605e58941f79404bd506da2c188f011046a339d0b069c1c97d0d?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/878cbec1206c605e58941f79404bd506da2c188f011046a339d0b069c1c97d0d?s=96&d=mm&r=g","caption":"Emily Humphreys"},"url":"https:\/\/admin.acceleratingscience.com\/author\/emilyhumphreys\/"}]}},"jetpack-related-posts":[],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/sites\/2\/2016\/10\/shutterstock_214525762-1.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/posts\/8870","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/comments?post=8870"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/posts\/8870\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/media\/8881"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/media?parent=8870"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/categories?post=8870"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/tags?post=8870"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/proteomics\/wp-json\/wp\/v2\/division?post=8870"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}