A New Strategy Optimized for Metabolite Profiling on Thermo Scientific Orbitrap ID-X Tribrid Mass Spectrometer Platform

A new data-dependent acquisition workflow is developed by using real-time background subtraction on a Thermo Scientific Orbitrap ID-X Tribrid mass spectrometer with AcquireX intelligent data acquisition strategy. This workflow enables collecting a complete dataset of high-resolution full MS and MSn scans of all potential metabolites in automated fashion, which needs neither pre-existing knowledge nor building exclusion and inclusion lists manually. Customized data-mining software is used for in-depth metabolite identification and structural elucidation. The results demonstrate that the Orbitrap ID-X MS with AcquireX data acquisition strategy coupled with advanced data processing software can improve metabolite analysis efficiency and quality.

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