Electropherogram of RNA transcripts of different sizes from two transcription systems:

1 kb, 4 kb, or 9 kb templates were used in the MEGAscript™ Kit or in another manufacturer's in-vitro transcription kit according to protocol. 20 µL reactions were diluted to 100 µL and 1 µL was assessed on the Agilent 2100 bioanalyzer. The single primary peak is indicative of product quality while the area under the peak is related to yield.