Figure 1. dsDNA specificity of PicoGreen dsDNA Reagent. Fluorescence enhancement of the Quant-iT PicoGreen dsDNA Reagent upon binding dsDNA, ssDNA, and RNA, demonstrating the strong selectivity of this reagent for dsDNA.
Quant-iT Reagents and Kits for >2,000 Samples
Best for reading more than 2,000 samples
These Quant-iT products provide the maximum amount of dye in packaging that is amenable to highly automated quantitation environments. These are the best choice for quantitating more than 2,000 samples at a time.
These Quant-iT products are available in two forms:
- Concentrated Quant-iT dyes
- Quant-iT kits with buffer and a standard included
For quantitation of 20–2,000 samples at a time, see our Quant-it Kits for Microplates.
Figure 2. Nucleic acid quantitation using Quant-iT Reagents. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation from 20 ng/ml to 1 μg/ml RNA using a 400-fold dilution of the dye; the inset shows the results for 1.0 ng/ml to 50 ng/ml RNA obtained using a 4,000-fold dilution of the dye. (Right) Quantitation of a synthetic 24-mer using the Quant-iT OliGreen ssDNA Reagent is linear over at least four orders of magnitude; the inset shows the results obtained using 0–2.0 ng/ml of oligo.
Qubit assays for use with the Qubit fluorometers
For everyday needs when quantitating 1–20 samples, use Invitrogen Qubit Assays for use with the Qubit fluorometers. Ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots and protein activity assays. See how easy it is to use the latest version of the Qubit - the Qubit 4 fluorometer.
- Learn more about Qubit assays
For Research Use Only. Not for use in diagnostic procedures.