Myc Tag Antibodies
The Myc tag, derived from the c-Myc protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. The Myc tag may be fused to either the N-terminus or C-terminus of a protein. The well-characterized Myc tag provides a reliable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
Invitrogen Myc tag antibodies are designed to dependably detect recombinant Myc-tagged proteins, and each antibody is validated for use in many applications.
Featured product data
Western blot analysis of c-Myc was performed by loading 25 µg of 293t cells transfected with c-Myc -MYCBP (lane 1), transfected with c-Myc -FLI1 (lane 2) and transfected with 12Tag (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a c-Myc Epitope Tag polyclonal antibody (Cat. No. PA1-981) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hour at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Cat. No. 32132). The image shows the target bands on c-Myc –MYCBP (19kDa), c-Myc -FLI1 (54kDa) and 12Tag (45kDa), which are consistent with predicted molecular weights for each target.
Flow cytometric analysis of c-Myc (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37°C. Cells were incubated with a c-Myc monoclonal antibody (Cat. No. MA1-980) or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500 µL of FACS buffer containing 10 µL of 4% paraformaldehyde, and analyzed on a flow cytometer.
Immunofluorescent analysis of c-Myc (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton™ X-100 for 15 minutes at room temperature. Cells were probed with a c-Myc monoclonal antibody (Cat. No. MA1-980) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.
Annotated product references
Cat. No. MA1-980-1MG was used in western blot to study the activation of the PRC-dependent stress program by apoptosis and senescence and its role in the response to cellular dysfunction. Gleyzer N, Scarpulla RC (2013) Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence. J Biol Chem 288:8004– 8015.
Cat. No. MA1-21316 was used in immunohistochemistry and western blot to study the cellular pathology of a CHMP2B missense mutation associated with neurodegeneration. Han JH, Ryu HH, Jun MH et al. (2013) The functional analysis of the CHMP2B missense mutation associated with neurodegenerative diseases in the endo-lysosomal pathway. Biochem Biophys Res Commun 421:544–549.
Cat. No. MA1-21316 was used in immunoprecipitation to study the interaction of the viral trans-frame P3N-PIPO protein with host PCaP1 and its role in potyvirus cell-to-cell movement. Vijayapalani P, Maeshima M, Nagasaki-Takekuchi N et al. (2012) Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement. PLoS Pathog 8:e1002639.
Cell analysis resources
- Cell Imaging Reagent Guide & Protocol App
- Fluorescence and Flow Cytometry Tutorials
- Antibodies for Flow Cytometry
- Antibodies for Western Blotting
- The Molecular Probes Handbook
- Cell Analysis Protocols
- The Cell Culture Basics Handbook
- Cell Analysis Technical Resources
- Labeling Chemistry Selection Tool
- Newsletters & Journals
For Research Use Only. Not for use in diagnostic procedures.