Celleste Image Analysis Software

Intuitive cell analysis at your fingertips

Get more data and insights from your captured images with powerful, easy-to-use software. Easily count, segment, classify and analyze complex images to create reports that include the parameters and data you need. Utilize automation tools to eliminate repetitive steps and minimize chances of errors or inconsistencies.

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Cell counting

Cell counting has traditionally been known to be laborious and inaccurate. With Celleste Image Analysis Software, cells can be counted in bright-field, colorimetric, or fluorescence mode. With the ability to capture cell counts over time, you can easily measure proliferation rates.

Count objects and create data tables with multiple parameter options
Figure 1. Count objects and create data tables with multiple parameter options.

Cell viability

Following cell viability over time can assist in evaluating the potency of cytotoxic compounds as part of cancer drug reasearch or just as part of good cell maintenance protocols.

Classification

Classification

Figure 2. Specify specific colors and names to classify and organize your measurements.

Cytoskeletal disruption

Time-lapse fluorescence microscopy on the EVOS FL Auto 2 Imaging System enables reliable and straightforward visualization of the cytoskeleton, in both fixed and live-cell systems.

Apoptosis

Membrane permeability is one of the first steps in the programmed cell-death pathway. It’s part of both normal and pathological processes. With the EVOS FL Auto 2 Imaging System and EVOS Onstage Incubator, induction of apoptosis and death can be measured over time by combining nuclear staining of membrane impermeable DNA stains and a caspase sensor.

Cell cycle

Quantitating cell numbers at various cell cycle checkpoints are integral for cancer research. Researchers looking for cell cycle developmental changes or modulators of the cell cycle can use Celleste Image Analysis Software to monitor for changes in intensity and color as cells go through the different cell cycle phases.

Wound healing

Tissue formation during embryonic development, wound healing, and immune responses all require the orchestrated movement of cells in particular directions to specific locations. Cells often migrate in response to specific external signals, including chemical signals and mechanical signals. An understanding of the mechanism by which cells migrate may lead to the development of novel therapeutic strategies for controlling, for example, invasive tumor cells. Combining the EVOS FL Auto 2 time-lapse live cell capabilities with the wound-healing measurement on Celleste software, allows generation of wound-healing data, over time, with the touch of a button.

Adipogenesis

The EVOS FL Auto 2 allows for the investigation of multiple factors that affect adipogenesis. For example, time-lapse images and Celleste software functionality can be combined to show increased adiposome number and size over time, as they are differentiated into adipocytes with differentiation media.

Colocalization

Colocalization

Figure 3. Easily export your measurements and data graphs for further analysis.

Q: Where can I find full specifications for Celleste software?
A: See the specifications on the Celleste Image Analysis Software product page.

Q: Is Celleste software compatible with images taken on any EVOS microscope?
A: Yes, Celleste software is compatible with all images taken from any EVOS system. For images taken on systems other than the EVOS FL Auto 2 instrument, spatial calibration is required for spatial data.

Q: Can Celleste software be installed on any computer?
A: Yes, as long as the computer meets specification requirements. Additionally, the activation dongle will need to be plugged in to the USB port of the computer in order to run Celleste software.

Q: How should I save my images on the EVOS FL Auto 2 system in order to perform quantitative analysis in Celleste software?
A: ​Be sure when saving images, they are saved as RAW images. We also recommend that you disable the High Brightness option in Settings to utilize the full bit depth of the system.

Q: ​​Can user accounts be set up in Celleste software so everyone may have their own settings?
A: Yes, go to Options and select Enable Application Configuration through User Logon at startup. This will allow you to create multiple user accounts when you click on the Administer Application Identifies button.

Q: Will Celleste software run on MAC or Linux operating systems?
A: ​No, Celleste software can only be run on Windows operating systems.

Learn how to set up batch processing to quickly analyze a set of images using Celleste Image Analysis software.

Learn how to write macros to set up automated analysis in Celleste Image Analysis software.


Learn how to count objects and display data for your images in Celleste Image Analysis software.

Learn how to set up the data collector tool in Celleste Image Analysis software to accumulate data on a series of images.


Learn how to calibrate your image metadata to measure distances on images in Celleste Image Analysis software.

Learn about the Learning Classification tool to classify objects on your images using a series of parameters in Celleste Image Analysis software.


Learn about how to use the Parent-Child app within Celleste Image Analysis software to study objects within an object.

Learn how to set up regions of interest on your images for analysis within Celleste Image Analysis software.


Learn how to use the Smart Segmentation tool within Celleste Image Analysis software to easily segment and count objects within your images.

Learn how to use the Tile Normalization app to correct the background of images within an area scan.

Cell counting

Cell counting has traditionally been known to be laborious and inaccurate. With Celleste Image Analysis Software, cells can be counted in bright-field, colorimetric, or fluorescence mode. With the ability to capture cell counts over time, you can easily measure proliferation rates.

Count objects and create data tables with multiple parameter options
Figure 1. Count objects and create data tables with multiple parameter options.

Cell viability

Following cell viability over time can assist in evaluating the potency of cytotoxic compounds as part of cancer drug reasearch or just as part of good cell maintenance protocols.

Classification

Classification

Figure 2. Specify specific colors and names to classify and organize your measurements.

Cytoskeletal disruption

Time-lapse fluorescence microscopy on the EVOS FL Auto 2 Imaging System enables reliable and straightforward visualization of the cytoskeleton, in both fixed and live-cell systems.

Apoptosis

Membrane permeability is one of the first steps in the programmed cell-death pathway. It’s part of both normal and pathological processes. With the EVOS FL Auto 2 Imaging System and EVOS Onstage Incubator, induction of apoptosis and death can be measured over time by combining nuclear staining of membrane impermeable DNA stains and a caspase sensor.

Cell cycle

Quantitating cell numbers at various cell cycle checkpoints are integral for cancer research. Researchers looking for cell cycle developmental changes or modulators of the cell cycle can use Celleste Image Analysis Software to monitor for changes in intensity and color as cells go through the different cell cycle phases.

Wound healing

Tissue formation during embryonic development, wound healing, and immune responses all require the orchestrated movement of cells in particular directions to specific locations. Cells often migrate in response to specific external signals, including chemical signals and mechanical signals. An understanding of the mechanism by which cells migrate may lead to the development of novel therapeutic strategies for controlling, for example, invasive tumor cells. Combining the EVOS FL Auto 2 time-lapse live cell capabilities with the wound-healing measurement on Celleste software, allows generation of wound-healing data, over time, with the touch of a button.

Adipogenesis

The EVOS FL Auto 2 allows for the investigation of multiple factors that affect adipogenesis. For example, time-lapse images and Celleste software functionality can be combined to show increased adiposome number and size over time, as they are differentiated into adipocytes with differentiation media.

Colocalization

Colocalization

Figure 3. Easily export your measurements and data graphs for further analysis.

Q: Where can I find full specifications for Celleste software?
A: See the specifications on the Celleste Image Analysis Software product page.

Q: Is Celleste software compatible with images taken on any EVOS microscope?
A: Yes, Celleste software is compatible with all images taken from any EVOS system. For images taken on systems other than the EVOS FL Auto 2 instrument, spatial calibration is required for spatial data.

Q: Can Celleste software be installed on any computer?
A: Yes, as long as the computer meets specification requirements. Additionally, the activation dongle will need to be plugged in to the USB port of the computer in order to run Celleste software.

Q: How should I save my images on the EVOS FL Auto 2 system in order to perform quantitative analysis in Celleste software?
A: ​Be sure when saving images, they are saved as RAW images. We also recommend that you disable the High Brightness option in Settings to utilize the full bit depth of the system.

Q: ​​Can user accounts be set up in Celleste software so everyone may have their own settings?
A: Yes, go to Options and select Enable Application Configuration through User Logon at startup. This will allow you to create multiple user accounts when you click on the Administer Application Identifies button.

Q: Will Celleste software run on MAC or Linux operating systems?
A: ​No, Celleste software can only be run on Windows operating systems.

Learn how to set up batch processing to quickly analyze a set of images using Celleste Image Analysis software.

Learn how to write macros to set up automated analysis in Celleste Image Analysis software.


Learn how to count objects and display data for your images in Celleste Image Analysis software.

Learn how to set up the data collector tool in Celleste Image Analysis software to accumulate data on a series of images.


Learn how to calibrate your image metadata to measure distances on images in Celleste Image Analysis software.

Learn about the Learning Classification tool to classify objects on your images using a series of parameters in Celleste Image Analysis software.


Learn about how to use the Parent-Child app within Celleste Image Analysis software to study objects within an object.

Learn how to set up regions of interest on your images for analysis within Celleste Image Analysis software.


Learn how to use the Smart Segmentation tool within Celleste Image Analysis software to easily segment and count objects within your images.

Learn how to use the Tile Normalization app to correct the background of images within an area scan.

Featured Product: EVOS FL Auto 2 Cell Imaging System

Thee EVOS FL Auto 2 Imaging System was designed with advanced capabilities to simplify demanding cell-based imaging applications—including live-cell imaging, image tiling, and Z-stacking—so researchers can focus on their data rather than instrument operation.

Learn more about the EVOS FL Auto 2 system

EVOS FL Auto 2