photo of the Attune NxT Flow Cytometer

Novel technology for precision and speed

Equipped with acoustic-assisted hydrodynamic focusing, the Invitrogen Attune NxT Flow Cytometer is able to achieve sample-throughput rates up to 10 times faster than traditional cytometers. This means that you can process samples more quickly and acquire sufficient events even from very dilute samples. In addition, the Attune NxT Flow Cytometer accommodates no-wash, no-lyse protocols, allowing you to collect data on samples that have been subjected to minimal handling.

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Learn more about the Attune NxT Flow Cytometer

"We have yet to clog the machine with our debris-rich primary tumor samples. Of course, the acoustic technology greatly facilitates the identification of small populations, like cancer stem cells, increasing our capacity to detect and quantify these rare events with high efficiency and reliability."

– Bruno Sainz Jr., PhD, Autonoma University of Madrid, School of Medicine

Acoustic focusing in action

This video demonstrates the event alignment that occurs when acoustic focusing is applied.

Faster sample processing compared to traditional cytometers

Equipped with acoustic-assisted hydrodynamic focusing, the Invitrogen Attune NxT Flow Cytometer processes samples faster than traditional cytometers. This breakthrough technology uses sound waves for more precise cell alignment and higher data quality. The pre-alignment of cells allows the Attune NxT Flow Cytometer to process samples quickly and acquires more statistically significant events even from very dilute samples.

Acoustic focusing offers many benefits including:

  • 10X faster than traditional flow cytometers
  • Broadens experimental capabilities by completing time-consuming experiments such as cell cycle analysis faster and without compromising data quality
  • Accommodates a broader range cell types, from small platelets to large cardiomyocytes
Acoustic flow cytometry
View from of the end of the acoustic-assisted flow cytometer's capillary with cells run at 500 µL/min.

Principles behind acoustic focusing

Flow cytometry data quality depends on collecting information from thousands of individual cells as they pass through the laser interrogation point. The acoustic technology within the Attune NxT Flow Cytometer generates ultrasonic waves that act to transport particles to the center of the sample stream. The pre-focused stream is injected into the sheath stream, which applies hydrodynamic pressure to the sample. The combination of these two forces results in a narrow core stream and an increased probability that cells are passing single file through optical system (Figure 1).

Acoustic-focusing cytometry

acoustic focusing principle

Figure 1. Overview of acoustic-assisted hydrodynamic focusing employed in the Attune NxT Flow Cytometer.

Acoustic technology maximizes quality and time

Traditional hydrodynamic focusing permits faster flow rates by broadening the width of the sample stream. This can be problematic as a wider core may allow more than one cell to pass through the laser at the same time (Figure 2B). Acoustic focusing does not broaden the core width to accommodate faster sample flow rates (Figure 2A). This type of system helps preserve data quality as single-cell interrogation is more likely to be maintained even at increased sample flow rates (Figure 2C).

Maximize quality and time

comparison of acoustic vs hydrodynamic focusing in terms of sample input rate
Figure 2. A wider distribution core typically means a broader distribution of cells. (A) Acoustic focusing technology allows for a narrow sample stream regardless of sample input rate. (B) Traditional hydrodynamic focusing requires widening the sample core for faster sample input rates. (C) Instruments equipped with acoustic-assisted technology can accommodate sample input rates from 12.5 to 1,000 µl/min.

Acoustic technology helps the Attune NxT Flow Cytometer achieve sample-throughput rates up to 10 times faster than traditional hydrodynamic focusing systems (Figures 3 and 4) and data acquisition speeds of 35,000 events/sec. Concentrated samples can be diluted and run quickly on the Attune NxT Flow Cytometer, offering your lab rapid high-quality data acquisition capabilities.

bar graph comparing speed of Attune NxT Cytometer to competitor instruments
Figure 3. Rapid data acquisition.The Attune NxT Flow Cytometer acquires 1,000,000 events in a fraction of the time required compared to three competitor instruments. Data represents protocol time achieved by running the flow cytometers at maximum sample rates for each instrument (1,000 µL/min for the Attune NxT Flow Cytometer).
Minimal data variation at high sample rates
Figure 4. Minimal data variation at high sample rates with the Attune NxT Flow Cytometer. Jurkat cells were fixed and stained with propidium iodide, treated with RNase and analyzed at a concentration of 1 x 106 cells/mL on the Attune NxT Flow Cytometer. Regardless of sample rate, the coefficient of variation (CV) of cells in the G0/G1 and G2/M phases remain consistent, even at the highest sample rate of 1,000 μL/min.

Faster acquisition times for the applications you’re already running

image of a scatter plot generated from the Attune NxT flow cytometer

We've conducted countless experiments using the Attune NxT Flow Cytometer, many of the same ones scientists are performing in their labs every day. Take a look at the data we've obtained for the following applications:

Analyze more cell types and maintain data integrity

Attune NxT Flow Cytometer handles cardiomyocytes and no wash, no lyse blood protocols
cell cycle analysis results from the Attune NxT Flow Cytometer
Rare-event detection data from the Attune NxT Flow Cytometer

Save time and minimize cell handling with faster protocols

The Attune NxT flow cytometer accommodates a no-wash, no-lyse protocol for whole blood, which dramatically reduces the number of steps you have to perform, the amount of sample that is lost during wash steps, and the amount of handling your cells have to endure. See the no wash, no lyse options sample data

Comparison of whole blood immunophenotyping protocols

  Number of steps Total sample prep time
Traditional immunophenotyping protocol
10 ~1–2 hr
No wash, no lyse immunophenotyping protocol 3 ~15–20 min

Download the application notes:

A Journey Through Innovation, The Discovery of Acoustic Focusing and the Attune Flow Cytometer
This mini-webinar discusses how the discovery of sound measurement lead to a series of discoveries culminating in the knowledge needed to use sound in particle focusing.

Principles behind acoustic focusing

Flow cytometry data quality depends on collecting information from thousands of individual cells as they pass through the laser interrogation point. The acoustic technology within the Attune NxT Flow Cytometer generates ultrasonic waves that act to transport particles to the center of the sample stream. The pre-focused stream is injected into the sheath stream, which applies hydrodynamic pressure to the sample. The combination of these two forces results in a narrow core stream and an increased probability that cells are passing single file through optical system (Figure 1).

Acoustic-focusing cytometry

acoustic focusing principle

Figure 1. Overview of acoustic-assisted hydrodynamic focusing employed in the Attune NxT Flow Cytometer.

Acoustic technology maximizes quality and time

Traditional hydrodynamic focusing permits faster flow rates by broadening the width of the sample stream. This can be problematic as a wider core may allow more than one cell to pass through the laser at the same time (Figure 2B). Acoustic focusing does not broaden the core width to accommodate faster sample flow rates (Figure 2A). This type of system helps preserve data quality as single-cell interrogation is more likely to be maintained even at increased sample flow rates (Figure 2C).

Maximize quality and time

comparison of acoustic vs hydrodynamic focusing in terms of sample input rate
Figure 2. A wider distribution core typically means a broader distribution of cells. (A) Acoustic focusing technology allows for a narrow sample stream regardless of sample input rate. (B) Traditional hydrodynamic focusing requires widening the sample core for faster sample input rates. (C) Instruments equipped with acoustic-assisted technology can accommodate sample input rates from 12.5 to 1,000 µl/min.

Acoustic technology helps the Attune NxT Flow Cytometer achieve sample-throughput rates up to 10 times faster than traditional hydrodynamic focusing systems (Figures 3 and 4) and data acquisition speeds of 35,000 events/sec. Concentrated samples can be diluted and run quickly on the Attune NxT Flow Cytometer, offering your lab rapid high-quality data acquisition capabilities.

bar graph comparing speed of Attune NxT Cytometer to competitor instruments
Figure 3. Rapid data acquisition.The Attune NxT Flow Cytometer acquires 1,000,000 events in a fraction of the time required compared to three competitor instruments. Data represents protocol time achieved by running the flow cytometers at maximum sample rates for each instrument (1,000 µL/min for the Attune NxT Flow Cytometer).
Minimal data variation at high sample rates
Figure 4. Minimal data variation at high sample rates with the Attune NxT Flow Cytometer. Jurkat cells were fixed and stained with propidium iodide, treated with RNase and analyzed at a concentration of 1 x 106 cells/mL on the Attune NxT Flow Cytometer. Regardless of sample rate, the coefficient of variation (CV) of cells in the G0/G1 and G2/M phases remain consistent, even at the highest sample rate of 1,000 μL/min.

Faster acquisition times for the applications you’re already running

image of a scatter plot generated from the Attune NxT flow cytometer

We've conducted countless experiments using the Attune NxT Flow Cytometer, many of the same ones scientists are performing in their labs every day. Take a look at the data we've obtained for the following applications:

Analyze more cell types and maintain data integrity

Attune NxT Flow Cytometer handles cardiomyocytes and no wash, no lyse blood protocols
cell cycle analysis results from the Attune NxT Flow Cytometer
Rare-event detection data from the Attune NxT Flow Cytometer

Save time and minimize cell handling with faster protocols

The Attune NxT flow cytometer accommodates a no-wash, no-lyse protocol for whole blood, which dramatically reduces the number of steps you have to perform, the amount of sample that is lost during wash steps, and the amount of handling your cells have to endure. See the no wash, no lyse options sample data

Comparison of whole blood immunophenotyping protocols

  Number of steps Total sample prep time
Traditional immunophenotyping protocol
10 ~1–2 hr
No wash, no lyse immunophenotyping protocol 3 ~15–20 min

Download the application notes:

A Journey Through Innovation, The Discovery of Acoustic Focusing and the Attune Flow Cytometer
This mini-webinar discusses how the discovery of sound measurement lead to a series of discoveries culminating in the knowledge needed to use sound in particle focusing.

Resources

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Flow Cytometry Learning Center
Access flow cytometry application resources for more success as you plan and execute your experiments.

Recommended learning

Fundamentals of Acoustic Cytometry

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