Obtain superior knockdown in multiple cell types

For your siRNA gene knockdown expieriments, you're getting:

  • A proprietary RNAi-specific cationic lipid formulation
  • Highest transfection efficiencies on the widest variety of cell types

Here we demonstrate strong mRNA knockdown in several lines using Silencer® Select siRNA in combination with Lipofectamine® RNAiMAX as the transfection agent.

Transfection of GAPDH with Lipofectamine® RNAiMAX into various cell types.

  % Knockdown relative to neg control
HASMC (human aortic smooth muscle) 98.1
HEKa (human epidermal keratinocytes, adult) 98.7
HRE (human renal epithelium) 98.5
NTERA-2 (cells-embryonal carcinoma) 98.7
HUVEC (human umbilical vein endothelium) 98.3
ADSC (adipose-derived stem cells) 97.6
HeLa 98.5
BJ (normal human fibroblast) 96.0
U2OS (osteosarcoma) 98.7
HEK 293 98.0
Control 0

Figure 1. Silencer® Select siRNAs delivered by reverse transfection using Lipofectamine® RNAiMax reagent induce strong GAPDH knockdown in multiple cell types.

Silencer® Select siRNAs for GAPDH were transfected at 30 nM using 0.15–0.3 µL of Lipofectamine® RNAiMAX reagent per well in 96-well plates, following reverse transfection for the various cell types. More than 95% knockdown was obtained for all cell types.

Order your sample; then, let us know which method has the highest TKO.