TaqMan® Sample-to-SNP™ Kit & GTXpress™ Master Mix
The TaqMan® Sample-to-SNP™ Kit provides a streamlined protocol for performing TaqMan® Assay-based genotyping analysis from virtually any sample without purifying DNA. These kits include GTXpress™ Master Mix, which is optimized for robust SNP genotyping results with crude sample lysates.
The TaqMan® Sample-to-SNP™ Kit takes you from biological sample to results in less than an hour, without isolating DNA. The kit is comprised of two parts: the DNA Extract All Lysis Reagents and the TaqMan® GTXpress™ Master Mix. The DNA Extract All Lysis Reagents reduce prolonged procedures for the release of real-time PCR-ready DNA to a 5 minute protocol. They are compatible with a wide variety of samples ranging from blood, to buccal swabs, to animal and plant tissues.
Lysates processed using the The TaqMan® GTXpress™ Master Mix enables robust PCR amplification in less than 50 minutes.
Many master mix products available today can perform reasonably well with highly purified DNA. However, even purified DNA from inhibitory samples such as blood or FFPE tissues can still pose challenges for these mixes. The TaqMan® GTXpress™ Master Mix has been formulated to handle a broad spectrum of inhibitors contained in samples as varied as blood and cotton.
- Fast—raw biological samples to SNP genotyping results in less than one hour
- Simple—a brief protocol with few pipetting steps
- Robust—highly accurate results for virtually any sample
- Flexible—validated with many types of TaqMan® SNP Genotyping Assays
TaqMan® Sample-to-SNP™ Kit Workflow
Figure 1: The DNA Extract All Lysis Reagents require only 5 minutes to release DNA into a lysate solution that is compatible with the TaqMan® GTXpress™ Master Mix. The TaqMan® GTXpress™ Master Mix enables robust PCR amplification in less than 50 min.
Clear Results From Multiple Sample Types
Figure 2: Samples of Buccal Swab (A), mouse tail (B), Corn leaf (C) and FFPE sample (D) were processed with the TaqMan® Sample-to-SNP™ Kit and the genotype results were obtained following the recommended user protocol. All amplifications and allelic discriminations were performed on an Applied Biosystems® 7900HT Fast Real-Time PCR System with a 384-well block.
Equivalent Genotyping Results Using Purified DNA & Lysate Prepared With the TaqMan® Sample-to-SNP™ Kit
Figure 3: Concordance of Genotyping Cluster Plots Using Purified DNA and Cell Lysate. The TaqMan® Sample-to-SNP™ Kit streamlines the SNP genotyping workflow by eliminating genomic DNA isolation. The kit was tested extensively for allele-call concordance between DNA-containing lysate and purified DNA. As shown here, TaqMan® Sample-to-SNP™ Kit lysate provides excellent cluster separation when compared to purified DNA. While the actual clustering is assay-dependent, a broad study comparing the genotyping call concordance between purified DNA and lysate across 46 EDTA-treated blood samples and two 'no template' controls showed excellent agreement. These 46 samples were genotyped with a 400 assay panel including 200 predesigned TaqMan® SNP Genotyping Assays, 100 TaqMan® Drug Metabolism Genotyping Assays and 100 Custom TaqMan® SNP Genotyping Assays.