Biotinylation

Biotinylation reagents and kits

We offer a wide range of biotin and desthiobiotin labeling reagents for targeting specific functional groups or residues, including primary amines and sulfhydryls on proteins, peptides, antibodies and other biomolecules. The strong biotin-avidin interaction is exploited to detect and/or purify proteins because of its high specificity.

Use our Biotinylation Reagent Selection Tool to find the optimal labeling reagent for your application, or access our helpful Bioconjugation Technical Handbook to help improve your biotin labeling results. Explore our Biotin Quantitation Reagents and HABA Calculator Tool to measure the amount of biotin attached biomolecule of interest.


Choose the right biotinylation reagent for your experiment based on reactive chemistry

Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-Link NHS-Biotin 13.5 No No Yes
EZ-Link NHS-Desthiobiotin 9.7 No No Yes
EZ-Link Sulfo-NHS-Biotin 13.5 No Yes No
EZ-Link NHS-LC-Biotin 22.4 No No Yes
EZ-Link Sulfo-NHS-LC-Desthiobiotin, No-Weigh Format 17.3 No Yes No
EZ-Link Sulfo-NHS-LC-Biotin 22.4 No Yes No
EZ-Link NHS-LC-LC-Biotin 30.5 No No Yes
EZ-Link Sulfo-NHS-LC-LC-Biotin 30.5 No Yes No
EZ-Link NHS-SS-Biotin 24.3 Yes No Yes
EZ-Link Sulfo-NHS-SS-Biotin 24.3 Yes Yes No
EZ-Link NHS-PEG4-Biotin 29 No Yes No
EZ-Link NHS-SS-PEG4-Biotin 37.9 Yes Yes Yes
EZ-Link PFP-Biotin 9.5 No No Yes
Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-Link BMCC-Biotin 32.6 No No Yes
EZ-Link HPDP-Biotin  29.2 No Yes Yes
EZ-Link Iodoacetyl-LC-Biotin 27.1 No No Yes
EZ-Link Iodoacetyl-PEG2-Biotin 24.7 Yes No No
EZ-Link Maleimide-PEG2-Biotin, No-Weigh Format 29.1 Yes No No
EZ-Link Maleimide PEG11-Biotin 59.1 Yes No No
EZ-Link TFP-PEG3-Biotin 32.6 Yes No No
Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-Link Amine-PEG2-Biotin 20.4 Yes No No
EZ-Link Amine-PEG3-Biotin 22.9 Yes No No
EZ-Link Amine-PEG11-Biotin 53.2 Yes No No
EZ-Link Biocytin 20.1 Yes No No
EZ-Link Pentylamine-Biotin 18.9  Yes No  No 
EZ-Link Biocytin 20.1 No Yes No
Oder products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-LinkTM Alkoxyamine-PEG12-Biotin 55.4 Yes No No
EZ-LinkTM Alkoxyamine-PEG4-Biotin 31.3  Yes No  No 
EZ-LinkTM Alkoxyamine-PEG4-SS-PEG4-Biotin 56.6 Yes Yes No
EZ-LinkTM Hydrazide-Biocytin 19.7 No No No
EZ-LinkTM Hydrazide-Biotin 15.7 No No Yes 
EZ-LinkTM Biotin-LC-Hydrazide 24.7 No No Yes
EZ-LinkTM Hydrazide-PEG4-Biotin 31.3 Yes No No
Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-Link Psoralen-PEG3-Biotin 36.9 Yes  No  No
EZ-Link TFPA-PEG3-Biotin 33.4 No No  Yes
Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-Link NHS-Biotin 13.5 No No Yes
EZ-Link NHS-Desthiobiotin 9.7 No No Yes
EZ-Link Sulfo-NHS-Biotin 13.5 No Yes No
EZ-Link NHS-LC-Biotin 22.4 No No Yes
EZ-Link Sulfo-NHS-LC-Desthiobiotin, No-Weigh Format 17.3 No Yes No
EZ-Link Sulfo-NHS-LC-Biotin 22.4 No Yes No
EZ-Link NHS-LC-LC-Biotin 30.5 No No Yes
EZ-Link Sulfo-NHS-LC-LC-Biotin 30.5 No Yes No
EZ-Link NHS-SS-Biotin 24.3 Yes No Yes
EZ-Link Sulfo-NHS-SS-Biotin 24.3 Yes Yes No
EZ-Link NHS-PEG4-Biotin 29 No Yes No
EZ-Link NHS-SS-PEG4-Biotin 37.9 Yes Yes Yes
EZ-Link PFP-Biotin 9.5 No No Yes
Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-Link BMCC-Biotin 32.6 No No Yes
EZ-Link HPDP-Biotin  29.2 No Yes Yes
EZ-Link Iodoacetyl-LC-Biotin 27.1 No No Yes
EZ-Link Iodoacetyl-PEG2-Biotin 24.7 Yes No No
EZ-Link Maleimide-PEG2-Biotin, No-Weigh Format 29.1 Yes No No
EZ-Link Maleimide PEG11-Biotin 59.1 Yes No No
EZ-Link TFP-PEG3-Biotin 32.6 Yes No No
Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-Link Amine-PEG2-Biotin 20.4 Yes No No
EZ-Link Amine-PEG3-Biotin 22.9 Yes No No
EZ-Link Amine-PEG11-Biotin 53.2 Yes No No
EZ-Link Biocytin 20.1 Yes No No
EZ-Link Pentylamine-Biotin 18.9  Yes No  No 
EZ-Link Biocytin 20.1 No Yes No
Oder products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-LinkTM Alkoxyamine-PEG12-Biotin 55.4 Yes No No
EZ-LinkTM Alkoxyamine-PEG4-Biotin 31.3  Yes No  No 
EZ-LinkTM Alkoxyamine-PEG4-SS-PEG4-Biotin 56.6 Yes Yes No
EZ-LinkTM Hydrazide-Biocytin 19.7 No No No
EZ-LinkTM Hydrazide-Biotin 15.7 No No Yes 
EZ-LinkTM Biotin-LC-Hydrazide 24.7 No No Yes
EZ-LinkTM Hydrazide-PEG4-Biotin 31.3 Yes No No
Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane Permeable?
EZ-Link Psoralen-PEG3-Biotin 36.9 Yes  No  No
EZ-Link TFPA-PEG3-Biotin 33.4 No No  Yes

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