Detergent Removal from Peptides

The Thermo Scientific Pierce Detergent Removal Resins are provided in convenient spin-column or plate formats that quickly and efficiently remove ionic, nonionic, and/or zwitterionic detergents from protein or peptide samples to improve compatibility with downstream applications. Two formulations are available that are optimized to remove detergents from peptide samples with different concentration ranges. The Thermo Scientific HiPPR (high protein and peptide recovery) products are recommended for peptide samples ≤100 μg/mL. The standard Pierce Detergent Removal Resin products are ideal for peptide samples >100 μg/mL.

  • High performance—removes detergent with >90% recovery and no sample dilution
  • Versatile—effectively removes a wide variety of detergents from peptide or protein samples
  • Optimized—separate formulations for samples with peptide concentrations ≤ or >100 μg/mL
  • Flexible—available in various formats, including spin columns, 96-well spin plates, and loose resin
  • Convenient—simple method that helps to improve MS peptide coverage

Choose the right peptide detergent removal product for your application

  HiPPR Detergent Removal Spin Columns HiPPR Detergent Removal Spin Kit HiPPR Detergent Removal Spin Plates Detergent Removal Spin Columns (4 sizes) Detergent Removal Resin Detergent Removal Spin Plates
  HiPPR detergent removal column HiPPR detergent removal spin kit HiPPR detergent removal spin plates Detergent Removal Spin Columns Detergent Removal Resin Detergent Removal Spin Plates
Sample volume range(s) 100 µL 25– 200 µL 100 µL
  • 10– 25 µL
  • 25– 100 µL
  • 150– 500 µL
  • 500– 1,000 µL
10– 2,500 µL 20– 100 μL
Recommended peptide sample concentration range 1– 100 µg/mL 1–100 µg/mL 1–100 µg/mL >100 µg >100 µg >100 µg
Format Pre-filled spin columns 5 mL resin + 0.8 mL empty spin columns 96-well pre-filled spin plate Pre-filled spin columns 10 mL resin 96-well pre-filled spin plate
Resin bed volume(s) 0.1 mL 0.025–0.2 mL 0.1 mL
  • 0.125 mL
  • 0.5 mL
  • 2 mL
  • 4 mL
0.125 mL– 10 mL 0.55 mL
Cat. No. 88306 88305 88307 87777 87780 88304

Table 1. Results using standard Thermo Scientific Pierce Detergent Removal Spin Column, 0.5 mL.

Detergent removal efficiency and protein recovery. BSA sample (25–200 µL) + detergent in 0.15 M NaCl, 0.05% sodium azide was mixed with equal volume of detergent removal resin (2x volume for CHAPS removal).

Detergent Sample volume (µL) Protein quantity (µg) Detergent removal (%) Protein recovery (%)
SDS (1%) 25 0.375 >99 98
50 0.75 >99 97
100 1.5 >99 100
200 3.0 >99 100
Triton X-100 (1%) 25 0.375 >95 82
50 0.75 >95 86
100 1.5 >95 86
200 3.0 >95 93
NP-40 (0.75%) 25 0.375 95 90
50 0.75 96 94
100 1.5 97 91
200 3.0 97 97
CHAPS (1%) 25 0.375 95 64
50 0.75 97 70
100 1.5 98 78
200 3.0 98 75

Table 2. Results using Thermo Scientific HiPPR Detergent Removal Resin.

Each column and plate well contained ~550 μL of detergent-removal resin slurry and 0.1 mL of sample.

Process format Detergent Detergent concentration (%) Detergent removal (%) BSA recovery (%)
0.5 mL Spin Column Sodium deoxycholate 5 99 100
Octyl glucoside 5 99 90
Octyl thioglucoside 5 99 95
Lauryl maltoside 1 98 99
Triton X-114 2 95 100
Brij-35 1 99 97
Tween 20 0.25 99 87
96-well Spin Plate SDS 5 99 89
Triton X-100 4 99 100
NP-40 1 95 100
CHAPS 5 99 100

 

Table 1. Results using standard Thermo Scientific Pierce Detergent Removal Spin Column, 0.5 mL.

Detergent removal efficiency and protein recovery. BSA sample (25–200 µL) + detergent in 0.15 M NaCl, 0.05% sodium azide was mixed with equal volume of detergent removal resin (2x volume for CHAPS removal).

Detergent Sample volume (µL) Protein quantity (µg) Detergent removal (%) Protein recovery (%)
SDS (1%) 25 0.375 >99 98
50 0.75 >99 97
100 1.5 >99 100
200 3.0 >99 100
Triton X-100 (1%) 25 0.375 >95 82
50 0.75 >95 86
100 1.5 >95 86
200 3.0 >95 93
NP-40 (0.75%) 25 0.375 95 90
50 0.75 96 94
100 1.5 97 91
200 3.0 97 97
CHAPS (1%) 25 0.375 95 64
50 0.75 97 70
100 1.5 98 78
200 3.0 98 75

Table 2. Results using Thermo Scientific HiPPR Detergent Removal Resin.

Each column and plate well contained ~550 μL of detergent-removal resin slurry and 0.1 mL of sample.

Process format Detergent Detergent concentration (%) Detergent removal (%) BSA recovery (%)
0.5 mL Spin Column Sodium deoxycholate 5 99 100
Octyl glucoside 5 99 90
Octyl thioglucoside 5 99 95
Lauryl maltoside 1 98 99
Triton X-114 2 95 100
Brij-35 1 99 97
Tween 20 0.25 99 87
96-well Spin Plate SDS 5 99 89
Triton X-100 4 99 100
NP-40 1 95 100
CHAPS 5 99 100