Cell cycle analysis with FxCycle Violet Ready Flow Reagent

Jurkat cells, a cycling human T cell leukemia cell line, were fixed in 4% paraformaldehyde and subsequently stained by adding 2 drops/mL of FxCycle™ Violet Ready Flow™ Reagent. The cells were then incubated for 30 minutes at 25°C. Data was acquired on an Attune™ NxT Flow Cytometer using a 405 nm laser. Emission was collected using a 440/50 nm filter.