Invitrogen Antibody Validation*
A commitment to antibody performance
Antibodies are some of the most critical research reagents used in the lab. Poor specificity or application performance can significantly frustrate the ability to obtain good results, which can cause critical delays. Underperforming antibodies result in a lack of reproducibility, wasting time and money. In other words, researchers need antibodies that bind to the right target and work in their applications every time. To help ensure superior antibody results, we've expanded our specificity testing methodology using a 2-part approach for advanced verification.
Invitrogen antibodies are currently undergoing a rigorous 2-part testing approach
Part 1—Target specificity verification
This helps ensure the antibody will bind to the correct target. Our antibodies are being tested using at least 1 of the following methods to ensure proper functionality in researcher’s experiments. Click on each testing method in the table below for detailed testing strategies, workflow examples and data figure legends.
|Genetic modification||Independent antibody verification (IAV)||Biological verification|
IP-mass spectrometry—testing using immunoprecipitation followed by mass spectrometry to identify antibody targets
IAV—measurement of target expression is performed using two differentially raised antibodies recognizing the same protein target
Cell treatment—detecting downstream events following cell treatment
Part 2—Functional application validation
These tests help ensure the antibody works in a particular application(s) of interest, which may include
(but are not limited to):
- Western blotting
- Flow cytometry
- Immunofluorescence imaging
Most antibodies were developed with specific applications in mind. Testing that an antibody generates acceptable results in a specific application is the second part of confirming antibody performance.
Learn more about our 2-part validation testing approach in this easy-to-read, one-page document. It summarizes our comprehensive verification testing process and describes how we provide a new standard of confidence for our customers.
Validation standards for reproducibility
Thermo Fisher Scientific is committed to adopting validation standards for our Invitrogen antibody portfolio that are tested for both target specificity and functional application. By supporting the International Working Group for Antibody Validation (IWGAV), and adopting their recommendations, we’re doing our part to ensure reproducibility and proper functionality in the scientific community.
Resources on antibody verification
Learn more about efforts from the research community to develop and implement standards for antibody validation.
Workshop report and session recordings
For Research Use Only. Not for use in diagnostic procedures.